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Academic literature on the topic '624.153 37'

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Published: 4 June 2021
Last updated: 16 February 2022

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Journal articles on the topic "624.153 37"

1

Wang, Andong, Xiaocong Li, Xianwen Luo, M. Santosh, Yurong Cui, Quanzhong Li, Dongrong Lai, Jianjun Wan, and Xuefen Zhang. "Crustal growth as revealed by integrated U–Pb and Lu–Hf isotope analyses of detrital zircons from the Ganjiang River, southeastern China." Geological Magazine 157, no. 4 (November 14, 2019): 666–76. http://dx.doi.org/10.1017/s001675681900116x.

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AbstractThe Ganjiang River, one of eight major tributaries of the Yangtze River, located in the western hinterland of the Cathaysia Block, SE China, has a length of 823 km and a drainage area of 82 809 km2, whose detrital zircons provide a valuable means to trace sediment provenances of the river and explore the crustal growth and evolution of the Cathaysia Block. In the current study, 389 concordia zircon U–Pb age spots and rare earth element (REE) contents, in combination with 201 Lu–Hf isotope analyses, have been determined. Oscillatory zoning, high Th/U ratios and REE distribution patterns indicate that most detrital zircon grains are of magmatic origin. The age can be further divided into seven groups: 130–185 Ma with a peak at 153 Ma (7 %); 217–379 Ma with a peak at 224 Ma (16 %); 390–494 Ma with a peak at 424 Ma (37 %); 500–698 Ma with a peak at 624 Ma (5 %); 716–897 Ma with a peak at 812 Ma (10 %); 902–1191 Ma with a peak at 976 Ma (13 %); and 2232–2614 Ma with a peak at 2471 Ma (5 %). The sources of almost all the zircon age groups can be found from the exposed rocks. In particular, Yanshanian, Hercynian to Indosinian, Pan-African, Grenvillian and Palaeoproterozoic–Archaean zircons can be mainly sourced from the northern Guangdong – southern Jiangxi – western Fujian region, while Caledonian zircons come from southern and central Jiangxi, and Jinningian zircons are from central and northern Jiangxi. Most determined zircon grains exhibit negative εHf(t) values and TDM2 ages of 797 to 4016 Ma with a wide peak at 1500–2100 Ma and a keen peak at 1824 Ma, suggesting that most zircons are sourced from the reworked ancient crustal materials or crust–mantle mixing. The zircon Hf model age cumulative probability diagram shows that rapid crustal growth took place at the Palaeo- to Mesoproterozoic and that about 90 % of the crust of the Cathaysia Block was formed before 1.5 Ga.
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Tsertsvadze, Tengiz, Amiran Gamkrelidze, Nikoloz Chkhartishvili, Akaki Abutidze, Lali Sharvadze, Vakhtang Kerashvili, Maia Butsashvili, et al. "Three Years of Progress Toward Achieving Hepatitis C Elimination in the Country of Georgia, April 2015–March 2018." Clinical Infectious Diseases 71, no. 5 (September 29, 2019): 1263–68. http://dx.doi.org/10.1093/cid/ciz956.

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Abstract Background In April 2015, in collaboration with the US Centers for Disease Control and Prevention and Gilead Sciences, the country of Georgia embarked on the world’s first hepatitis C elimination program. We aimed to assess progress toward elimination targets 3 years after the start of the elimination program. Methods We constructed a hepatitis C virus (HCV) care cascade for adults in Georgia, based on the estimated 150 000 persons aged ≥18 years with active HCV infection. All patients who were screened or entered the treatment program during April 2015–March 2018 were included in the analysis. Data on the number of persons screened for HCV were extracted from the national HCV screening database. For the treatment component, we utilized data from the Georgia National HCV treatment program database. Available treatment options included sofosbuvir and ledipasvir/sofosbuvir–based regimens. Results Since April 2015, a cumulative 974 817 adults were screened for HCV antibodies; 86 624 persons tested positive, of whom 61 925 underwent HCV confirmatory testing. Among the estimated 150 000 adults living with chronic hepatitis C in Georgia, 52 856 (35.1%) were diagnosed, 45 334 (30.2%) initiated treatment with direct-acting antivirals, and 29 090 (19.4%) achieved a sustained virologic response (SVR). Overall, 37 256 persons were eligible for SVR assessment; of these, only 29 620 (79.5%) returned for evaluation. The SVR rate was 98.2% (29 090/29 620) in the per-protocol analysis and 78.1% (29 090/37 256) in the intent-to-treat analysis. Conclusions Georgia has made substantial progress in the path toward eliminating hepatitis C. Scaling up of testing and diagnosis, along with effective linkage to treatment services, is needed to achieve the goal of elimination.
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Nadezhdina, Elena Y., Zhanna E. Belaya, Ludmila Y. Rozhinskaya, Vilen N. Azyzjan, Oksana V. Ivashenko, Aleksander V. Ilyin, Galina S. Kolesnikova, Irina V. Stanoevich, Anastasia M. Lapshina, and Andrey Y. Grigoriev. "The role of intrа and postoperative ACTH and cortisol levels measurement in patients with Cushing’s disease as an early predictors of remission." Endocrine Surgery 11, no. 1 (July 7, 2017): 28–37. http://dx.doi.org/10.14341/serg2017128-37.

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Цель работы. Изучение динамического изменения интра- и ранних послеоперационных гормональных показателей (адренокортикотропного гормона (АКТГ) и кортизола) в качестве предикторов ремиссии гиперкортицизма. Материал и методы. Проведен сравнительный анализ результатов эндоскопической аденомэктомии у 50 пациентов с болезнью Иценко–Кушинга. Пациенты были разделены на три группы в зависимости от исхода операции. Первая группа – пациенты с вторичной надпочечниковой недостаточностью, подтвержденной клинической картиной и уровнем кортизола меньше 50 нмоль/л; вторая группа – с нормализацией уровней АКТГ и кортизола; третья – с персистенцией болезни Иценко–Кушинга. Оценена динамика снижения уровней АКТГ и кортизола во время операции и в раннем послеоперационном периоде. Группу составили 38 женщин и 12 мужчин в возрасте от 15 до 66 лет. Заборы крови для оценки уровней АКТГ и кортизола производились из периферической вены: первый – на этапе разреза твердой мозговой оболочки, второй – сразу после удаления опухоли и заключительный – через 20 мин после удаления аденомы. Затем исследование вышеуказанных гормонов проводилось всем пациентам через сутки после операции. Уровни АКТГ и кортизола определялись путем иммунохемилюминесцентного анализа на автоматизированной системе Сobas 6000 (Roche, Франция). Референсные интервалы АКТГ – 0–30 нг/мл, кортизола – 123–626 нмоль/л. Результаты. Не получено четкой зависимости между изменениями уровня исследуемых гормонов в интра- и раннем послеоперационном периоде (20 мин после удаления опухоли) и вероятностью наступления ремиссии заболевания (p > 0,0125). Результаты гормонального исследования через сутки коррелировали с частотой послеоперационной ремиссии (p < 0,0125). В послеоперационном периоде из 50 пациентов у 41 (82%) развилась надпочечниковая недостаточность, у 5 (10%) пациентов показатели нормализовались и у 4 (8%) пациентов наблюдалась персистенция гиперкортицизма. Заключение. Интраоперационное определение уровней АКТГ и кортизола не является целесообразным для оценки радикальности удаления опухоли.
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Nielsen, Chloe, Kelly Zafman, Nathan Fox, and Emily Miller. "Optimal Timing of Delivery in Women with Higher Order Cesareans: A Cohort Study." American Journal of Perinatology 35, no. 12 (April 16, 2018): 1154–58. http://dx.doi.org/10.1055/s-0038-1641587.

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Objective To evaluate whether a planned early term delivery or a planned 39-week delivery is associated with differences in perinatal outcomes in women undergoing a higher order cesarean (HOC). Study Design This cohort study included women with singleton gestations with a history of three or more prior cesareans who delivered at one of two urban tertiary care hospitals. One center routinely delivered HOC at 39 weeks' gestation and the other at 37 weeks. Maternal and neonatal morbidities were compared using bivariable and multivariable analyses. Results The policy of 37-week delivery was associated with a decrease in unscheduled deliveries (15.3 vs. 41.1%; p < 0.001). Planned delivery at 37 weeks was associated with a decreased incidence of composite maternal morbidity (1.6 vs. 7.9%; p = 0.002) and 5-minute Apgar score less than 7 (0.4 vs. 6.4%; p < 0.001), but these differences were not significant after controlling for potential confounders (adjusted odds ratio [aOR]: 0.30, 95% confidence interval [CI]: 0.08–1.17; aOR: 0.13, 95% CI: 0.01–1.30, respectively). There were no other differences in perinatal outcomes. Conclusion Compared with planned delivery at 39 weeks, a policy of planned delivery at 37 weeks was associated with a reduction in unscheduled deliveries, but there were no measured differences in perinatal outcomes.
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Fizazi, Karim, Philippe Beuzeboc, Jean Lumbroso, Vincent Haddad, Christophe Massard, Marine Gross-Goupil, Mario Di Palma, et al. "Phase II Trial of Consolidation Docetaxel and Samarium-153 in Patients With Bone Metastases From Castration-Resistant Prostate Cancer." Journal of Clinical Oncology 27, no. 15 (May 20, 2009): 2429–35. http://dx.doi.org/10.1200/jco.2008.18.9811.

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PurposeTo assess docetaxel combined with samarium-153–ethylene diamine tetramethylene phosphonic acid (EDTMP), a radiopharmaceutical with a high affinity for bone, in patients with castration-resistant prostate cancer (CRPC).Patients and MethodsPatients with bone metastases from CRPC who achieved a response or stabilization after four cycles of docetaxel and estramustine were given consolidation docetaxel 20 mg/m2/wk for 6 weeks and samarium-153-EDTMP (37 MBq/kg) during week 1. Prostate-specific antigen (PSA) response was assessed by using consensus criteria, and pain was assessed by using a visual analog scale (VAS). This study used a Simon two-step design with PSA–progression-free survival (PFS) as the primary end point.ResultsForty-three patients were included in the trial. A PSA response was obtained in 77% (95% CI, 61% to 82%). The pain response rate was 69% (95% CI, 49% to 85%). At least five of the six planned weekly injections of docetaxel were administered to 34 patients (81%). The consolidation docetaxel–samarium-153–EDTMP regimen was well tolerated; there was no febrile neutropenia, and only two episodes (5%) of rapidly reversible grade 3 thrombocytopenia occurred. Although a serum PSA relapse eventually occurred in all patient cases, this regimen resulted in pain control in the long-term. The median PSA-PFS was 6.4 months (95% CI, 6 to 7 months). The median survival was 29 months (95% CI, 22 to 31); the 1-year survival rate was 77% (62% to 87%); and the 2-year survival rate was 56% (41% to 70%).ConclusionCombining docetaxel and samarium-153–EDTMP in patients with bone metastases from CRPC is well tolerated, and it yields major pain relief that persists long after treatment. Overall survival compares favorably with that expected in this population of patients, most of whom exhibit symptoms.
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Tigabu, Abiye, Moges Tiruneh, and Feleke Mekonnen. "Nasal Carriage Rate, Antimicrobial Susceptibility Pattern, and Associated Factors of Staphylococcus aureus with Special Emphasis on MRSA among Urban and Rural Elementary School Children in Gondar, Northwest Ethiopia: A Comparative Cross-Sectional Study." Advances in Preventive Medicine 2018 (December 11, 2018): 1–11. http://dx.doi.org/10.1155/2018/9364757.

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Introduction. Staphylococcus aureus is a Gram-positive, catalase-positive, and coagulase-positive bacterial species commonly found on the skin and in the nose of most healthy individuals. The anterior nares of nose are the most frequent carriage sites for S. aureus in both adults and children. Methicillin resistance among S. aureus isolates has steadily increased worldwide. Objective. The main objective of this study was to determine nasal carriage rate, antimicrobial susceptibility pattern, and associated risk factors of Staphylococcus aureus with special emphasis on MRSA among urban and rural elementary school children in Gondar, Northwest Ethiopia. Method. A community based comparative cross-sectional study was conducted on 622 urban and rural elementary school children in Gondar from January 1st to March 30th, 2018. Data was collected using a questionnaire and nasal swab samples were collected by sterile cotton tip swab moistened with sterile normal saline. Collected samples were inoculated on mannitol salt agar and incubated aerobically at 37°C for 24 hrs. S. aureus was confirmed by observing colony characteristics and biochemical tests. MRSA was detected using cefoxitin disc by Modified Kirby-Bauer disk diffusion technique. Finally data was entered, cleared, and checked using Epi-info version 7 and exported to SPSS version 20 for analysis. Odds ratio and logistic regression were used for statistical association. P-value ≤ 0.05 at 95% CI was considered for statistical association. Result. Of the 622 school children, the overall prevalence of S. aureus was 143/622 (23%). Of them, 14/143 (9.79%) were MRSA. The carriage rate in urban schools was 83/622 (13.3%) whereas it was 60/622 (9.6%) in rural schools. The prevalence of MRSA among urban schools, 9.1%, was higher than their urban counterparts, 0.7%. Gentamycin, clindamycin, and ciprofloxacin were the most effective whereas penicillin and tetracycline were resistant. Children’s fathers’ educational status and number of children in class room were significantly associated with S. aureus but only living in urban of children significantly associated with MRSA. Conclusion. This study showed high prevalence of S. aureus and MRSA, 143/622 (23%) and 14/143 (9.79%), respectively. So, decolonization of nasal carriers of MRSA and reducing the number of students per classroom should be addressed. Moreover, regular large scale survey should be conducted to assess the burden and intervene accordingly.
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Goyal, Rakesh K., Liane Fairfull, Robert B. Gerbing, Todd A. Alonzo4, Soheil Meshinchi4, Beverly J. Lange4, William J. Woods4, and Robert E. Ferrell. "Calcitonin A Genotype Is Associated with Risk of Acute Graft-Versus-Host Disease Following Allogeneic Bone Marrow Transplantation for Children with Acute Myeloid Leukemia in First Remission." Blood 106, no. 11 (November 16, 2005): 1813. http://dx.doi.org/10.1182/blood.v106.11.1813.1813.

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Abstract Background: Procalcitonin (PCT) is a 13 kD 116-amino acid prohormone. Elevated serum levels of procalcitonin have been noted in patients with bacterial infections such as septicemia, pneumonia and immune-mediated conditions like graft-versus-host disease (GvHD) and organ rejection. In this study, we sought to examine if a single base pair polymorphism (a T to C transition) at position −624 of the calcitonin A gene (CALCA) was associated with risk of acute GvHD. Subjects: 113 children (median age 10 y; range 0.38 – 19.0 y) with de novo acute myeloid leukemia (AML) treated on recent Children’s Oncology Group protocols 2891, 2941 or 2961. All of these patients received matched sibling bone marrow transplant (BMT) in first clinical remission. Ablative conditioning consisted of busulfan and cyclophosphamide. All patients received methotrexate until day 100 for GvHD prophylaxis. Methods: All clinical DNA specimens were subjected to whole genome amplification. Genotyping was performed using the 5′-nuclease (TaqMan) assay on the ABI 7700 DNA analyzer (Applied Biosystems). Results: Observed CALCA -624 T&gt;C genotypes in 113 patients were: TT = 52 (46%), TC = 43 (38%) and CC = 18 (16%); T allele = 73 (65%) and C allele = 40 (35%). There were no significant differences in patient characteristics (age, gender, race, study 2891/2941/2961, white blood count at diagnosis, FAB type, or cytogenetics) between the TT vs. combined TC or CC groups. Overall, acute GvHD grade I–IV (Seattle criteria) was observed in 55/113 patients (49%). Nineteen out of 52 (37%) with TT, while 21 out of 43 (49%) with TC, and 15 out of 18 (83%) with CC CALCA genotypes developed acute GvHD. Alternatively, acute GvHD was observed in 37% patients with common TT genotype and in 59% patients with variant TC or CC genotype (p=0.023). The odds ratio for developing acute GvHD (grade I – IV) for children with TC or CC genotypes was 2.5 (CI 1.17, 5.33) compared with those with TT genotype. The difference in observed acute GvHD remained significant when the analyses were limited to more uniform subgroups: 54 patients on single trial, 2961 only (p=0.028) and 42 whites only on 2961 trial (p=0.028). Severe acute GvHD (grade III – IV) was seen in 6/52 (12%) patients with TT vs. 6/61 (10%) in those with TC or CC genotypes; this difference was not statistically significant (p =0.771). There were no significant differences in day +100 transplant related deaths, engraftment, relapse rate or overall survival. Plans: Association between CALCA genotypes and infectious complications will be also analyzed when clinical data abstraction is completed. CALCA −1752 C&gt;G and 3740 A&gt;G genotyping is in progress and clinical correlative analyses will be repeated at genotype and haplotype levels.
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Susilowati, Dewi, Niken Subekti, and Siti Harnina Bintari. "The Potential of Microbial Symbionts Macrotermes gilvus Hagen Termite Gut as Degrading Agents of Cellulose in Bioethanol Production." Biosaintifika: Journal of Biology & Biology Education 10, no. 2 (August 29, 2018): 396–401. http://dx.doi.org/10.15294/biosaintifika.v10i2.14965.

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Water hyacinth is a potential feedstock for bioethanol production because of their high cellulose. The microbial symbionts of the Macrotermes gilvus termite’s gut have a high endoglucanase enzyme activity. This research was aimed to analyze the pH, temperature and agitation effects towards cell density, endoglucanase enzyme activity and reducing sugar, and to determine the effective optimum condition that can produce maximum reducing sugar. This research used central composite design (CCD) with the total number of run was . The independent variables were including pH (5.9, 6.4, 7.0, 7.6, 8.0), temperature (30 0C, 33 0C, 37 0C, 41 0C, 44 0C) and agitation (90 rpm,114 rpm, 150 rpm,185 rpm, 210 rpm), with six replications at central points. Parameters measured were cell density, endoglukanase enzyme activity and reducing sugar, thus analyzed by the statistical software package MINITAB 18.0. The Student’s t-test result showed the primary sequence influencing cell density as pH ˃ agitation ˃ temperature and towards endoglucanase enzyme activity and reducing sugar as pH ˃ temperature ˃ agitation, P ˂ 0.05. The maximum reducing sugar (60.13 ± 3.16 mmolL-1) was obtained at pH 6.95, temperature 37 0C and agitation 150 rpm. The results of this research can be used to explore the more potential microbial symbionts of the Macrotermes gilvus Hagen termite’s gut.
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Das, Subhas, and Dileep Kumar Singh. "Purification and characterization of phosphotriesterases from Pseudomonas aeruginosa F10B and Clavibacter michiganense subsp. insidiosum SBL11." Canadian Journal of Microbiology 52, no. 2 (February 1, 2006): 157–68. http://dx.doi.org/10.1139/w05-113.

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A microbial biodegradation of monocrotophos was studied in the present investigation. The monocrotophos-degrading enzyme was purified and characterized from two soil bacterial strains. The cells were disrupted and the membrane-bound fractions were studied for purification and characterization. Solubilization of the membrane-bound fractions released nearly 80% of the bound protein. Phase separation further enriched the enzyme fraction 34–41 times. The enzyme phosphotriesterase (PTE) from both the strains was purified to more than 1000-fold with 13%–16% yield. Purified PTE from Clavibacter michiganense subsp. insidiosum SBL11 is a monomeric enzyme with a molecular mass of 43.5 kDa (pI of 7.5), while PTE from Pseudomonas aeruginosa F10B is a heterodimeric enzyme with a molecular mass of 43 and 41 kDa (pI of 7.9 and 7.35). Both purified enzymes are stable enzymes with peak activity at pH 9.0. The enzyme from strain F10B was more thermostable (half-life = 7.3 h) than that from SBL11 (half-life = 6.4 h at 50 °C), while both showed the same temperature optimum of 37 °C. Inhibitors like dithiothreitol and EDTA inhibited the purified enzyme, while p-chloromercuribenzoic acid and indoleacetic acid had a very little effect.Key words: biodegradation, monocrotophos, phosphotriesterase, Pseudomonas aeruginosa F10B, Clavibacter michiganense subsp. insidiosum SBL11.
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Schadewaldt, Peter, Annette Bodner-Leidecker, Hans-Werner Hammen, and Udo Wendel. "Significance of l-Alloisoleucine in Plasma for Diagnosis of Maple Syrup Urine Disease." Clinical Chemistry 45, no. 10 (October 1, 1999): 1734–40. http://dx.doi.org/10.1093/clinchem/45.10.1734.

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Abstract Background: The significance of plasma l-alloisoleucine, which is derived from l-isoleucine in vivo, for diagnosis of maple syrup urine disease (MSUD) was examined. Methods: Branched-chain l-amino acids were measured by automatic amino acid analysis. Results: Alloisoleucine reference values in plasma were established in healthy adults [1.9 ± 0.6 μmol/L (mean ± SD); n = 35], children 3–11 years (1.6 ± 0.4 μmol/L; n = 17), and infants &lt;3 years (1.3 ± 0.5 μmol/L; n = 37). The effect of dietary isoleucine was assessed in oral loading tests. In controls receiving 38 μmol (n = 6; low dose) and 1527 μmol (n = 3; high dose) of l-isoleucine per kilogram of body weight, peak increases of plasma isoleucine were 78 ± 24 and 1763 ± 133 μmol/L, respectively; the peak increase of alloisoleucine, however, was negligible for low-dose (&lt;0.3 μmol/L) and minor for high-dose (5.5 ± 2.1 μmol/L) load. In patients with diabetes mellitus, ketotic hypoglycemia, phenylketonuria, and obligate heterozygous parents of MSUD patients, alloisoleucine was not significantly different from healthy subjects. Therefore, a plasma concentration of 5 μmol/L was used as a cutoff value. In patients with classical MSUD (n = 7), alloisoleucine was beyond the cutoff value in 2451 of 2453 unselected samples. In patients with variant MSUD (n = 9), alloisoleucine was &gt;5 μmol/L in all samples taken for establishment of diagnosis and in 94% of the samples taken for treatment control (n = 624). With the other branched-chain amino acids, the frequency of diagnostically significant increases was &lt;45%. Conclusions: The present findings indicate that plasma l-alloisoleucine above the cutoff value of 5 μmol/L is the most specific and most sensitive diagnostic marker for all forms of MSUD.
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Book chapters on the topic "624.153 37"

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"Fuller, L, ‘Consideration and form’ (1941) 41 Columbia LR 799. Fuller, L and Perdue, W, ‘The reliance interest in contract damages’ (1936–37) 46 Yale LJ 52. Furmston, M, ‘Return to Dunlop v Selfridge?’ (1960) 23 MLR 373. Gardner, S, ‘Trashing with Trollope: a deconstruction of postal rules in contract’ (1992) 2 OJLS 170. Goff, R, and Jones, G, The Law of Restitution, 4th edn, 1993, London: Sweet & Maxwell. Gower, L, ‘Exemption clauses – contractual and tortious liability’ (1954) 17 MLR 155. Hamson, C, ‘The reform of consideration’ (1938) 54 LQR 233. Harris, D, Ogus, A and Phillips, J, ‘Contact remedies and the consumer surplus’ (1979) 95 LQR 581. Harrison, Good Faith in Sales, 1997, London: Sweet & Maxwell. Howarth, W, ‘The meaning of objectivity in contract’ (1984) 100 LQR 265. Jacobs, J, ‘The battle of the forms: standard term contracts in comparative perspective’ (1985) 34 ICLQ 297. Kessler, ‘Contracts of adhesion: some thoughts about freedom of contract’ (1943) Columbia LR 629. Law Commission, First Report on Exemption Clauses in Contracts, Law Com No 24, 1969, London: HMSO. Law Commission, Firm Offers, 1975, Working Paper No 60, London: HMSO. Law Commission, Second Report on Exemption Clauses in Contracts, Law Com No 69, 1975, London: HMSO. Law Commission, Law of Contract: The Parol Evidence Rule, Law Com No 154, Cmnd 9700, 1986, London: HMSO. Law Commission, Privity of Contract: Contracts For The Benefit of Third Parties, Law Com No 242, Cm 3329, 1996, London: HMSO." In Sourcebook on Contract Law, 809. Routledge-Cavendish, 1995. http://dx.doi.org/10.4324/9781843141518-323.

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Conference papers on the topic "624.153 37"

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Lewis, J. C., M. J. Greene, R. G. Taylor, and R. R. Hantgan. "THROMBASTHENIC-LIKE DEFECT IN PLATELETS OF THE AFRICAN GREEN MONKEY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644557.

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African green monkeys, an animal model widely used in cardiovascular research, have platelets which ultrastructurally and functionally are similar to man. Through clinical screening of animals in a controlled breeding colony a congenital defect has been identified in platelets from an adolescent AG monkey, AJ403. The defect was characterized as abortive primary aggregation with ADP in the range 1.25-20.0 μM and PAF at concentrations to 5 × 10-6 M. Ultrastructurally, platelets within the aggregates had pseudopods, centralized granules and evidence of degranulation. Granule content release was verified using the luciferin-luciferase reaction in the Lumi-aggregometer. Platelet-platelet binding and close apposition, typically found with full primary and secondary aggregation, were not observed. Using a standardized in vitro assay at 37°C for 15-45 minutes, adhesion to formvar coated srufaces with AJ403 was 10-20% of control. Adherent cells had few pseudopods, minimal elaboration of the hyalomere, and limited cytoskeletal polymerization. Since the aggregation and adhesion patterns paralleled those described for Glanzmann's thrombasthenia, fibrinogen binding was quantitated using 125I fibrinogen and was ultrastructurally evaluated using fibrinogen-gold conjugates. Following ADP stimulation in the presence of fibrinogen at 0.08, 0.16 and 0.24 mg/ml, normal platelets respectively bound 64, 81 and 106 × 103 molecules fibrinogen/cell whereas platelets from AJ403 bound 28, 43 and 52 × 103 molecules/cell. This correlated with the number of gold/fibrinogen conjugates bound to the platelet surface which with AJ403 were less than 40% of control. Our observations suggest a parallel of AJ403 with Type II Thrombasthenia in man.
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Bernaciková, Martina, Jakub Mazúr, Martin Sebera, and Petr Hedbávný. "Monitoring Heart Rate Variability As A Biomarker Of Fatigue In Young Athletes." In 12th International Conference on Kinanthropology. Brno: Masaryk University Press, 2020. http://dx.doi.org/10.5817/cz.muni.p210-9631-2020-21.

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Purpose: Many high performance and especially top athletes are still at risk or suffer from total fatigue. Therefore, sports science seeks to develop an objective, sensitive and reliable method of early diagnosis of this fatigue (e.g. heart rate variability – HRV as a modern ob-jective method). The aim of the study was to evaluate whether the HRV monitoring could be a complementary diagnostic tool for overreaching / overtraining in young athletes. Already introduced “classical” indicators of HRV, such as spectral performance and its density in the established frequency ranges, are a part of athlete monitoring in the scope of overreaching prevention We were monitoring the heart rate variability parameters at three different phases of the year-long training cycle and to find out whether in one of these phases we could find athletes showing symptoms of overreaching. Methods: 48 young athletes (33 boys 14.8 ± 1.5 years, 15 girls 14.9 ± 1.7 years) were involved in the study, consisting of 38 boys and 10 girls. There were 15 swimmers (with training volume 9x 1.5‒2 hours a week), 12 artistic gymnasts (with training volume 9x 2‒2.5 hours a week) and 21 badminton players (with training volume 4x weekly 1.5‒2 hours a week). Monitoring was carried out in athletes in three training periods: at the end of the transition period, at the end of the prepared period, at the end of the competition period. Measurements were carried out in the morning. The DiANS PF8 system was used to measure the heart rate variability, the measurements were performed at five-minute intervals: lying-standing-lying. Time and spectral parameters of HRV were monitored. Results: Results of HRV in three periods (HR + rMSSD in lying). Boys: HR (61 ± 8, 64 ± 7, 64 ± 8), rMSSD (85 ± 64; 80 ± 54; 88 ± 59), TS (-0.56 ± 1.53; -0.87 ± 1.4; -0.42 ± 1.44). Girls: HR (65 ± 8; 64 ± 7; 65 ± 8), rMSSD (74 ± 37; 79 ± 35; 83 ± 43), TS (-0.58 ± 1.57; -0.72 ± 1.35); -0.18 ± 0.18). Statistically significant differences (at the significance level = 0.05) among sports were found in Kruskal-Walls ANOVAby Ranks: boys in LF-standing, HF standing, FV, SVB and TS; girls in HF-lying, HF-standing, rMSSD, TP-lying, TP-standing, FV, VA and TS. Conclusion: Monitoring of heart rate variability seems to be a practical tool for prevention of overtraining even in young age. To monitor heart rate variability, we recommend monitoring these parameters: RR, rMSSD, VA, SVB, TS.
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