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1

Henzi, Maria X., David L. McNeil, Mary C. Christey, and Ross E. Lill. "A tomato antisense 1-aminocyclopropane-1-carboxylic acid oxidase gene causes reduced ethylene production in transgenic broccoli." Functional Plant Biology 26, no. 2 (1999): 179. http://dx.doi.org/10.1071/pp98083.

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In this paper 11 transgenic broccoli (Brassica oleracea L. var. italica) lines containing a tomato antisense 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene from pTOM13 were evaluated. Changes in respiration, ethylene production and ACC oxidase activity were studied in mature flowers. Averaged across all ACC oxidase transgenic lines, there was an initial increase followed by a substantial decrease in ethylene production compared with the controls. Of the 11 transgenic lines, 10 lines showed a significant reduction in fethylene production relative to the controls from 50 h after harves
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2

Perkins-Veazie, P., J. K. Collins, V. Russo, and B. Cartwright. "Aphids Stimulate Peroxidase Activity but Not ACC Oxidase Activity in Watermelon Plants Inoculated with Anthracnose." HortScience 31, no. 4 (1996): 677d—677. http://dx.doi.org/10.21273/hortsci.31.4.677d.

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Individually, green melon aphids (Aphis gossypi) and anthracnose (Colletotricum lagenarium) can cause serious economic damage to watermelons by reducing stands and marketable yields. Greenhouse-grown watermelon seedlings at the third true leaf stage were infected with anthracnose (106 spores/mL) and/or infested with 30 aphids per plant. At the 5th leaf stage (about 7 days after inoculation/infestation), leaf disks were harvested from plants and indicators of stress measured. Peroxidase activity increased from 0.03 to 0.28 absorbance units/mg protein-minute in leaves with anthracnose. When plan
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3

Emery, R. J. N., A. Kathiresan, D. M. Reid, and C. C. Chinnappa. "Ecotypic differences in rhythmicity of ethylene production in Stellaria longipes: the possible roles of ACC, MACC, and ACC oxidase." Canadian Journal of Botany 75, no. 7 (1997): 1027–33. http://dx.doi.org/10.1139/b97-114.

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The alpine tundra ecotype of Stellaria longipes is characterized by a dwarf phenotype, whereas the prairie ecotype can be semidwarf or highly elongated depending on its environment. Related to their ability to elongate, these ecotypes also show divergent abilities to produce and respond to ethylene. The prairie ecotype produces a strong daily rhythm of ethylene, which is maintained even following stress events such as wind. The alpine ecotype exhibits a much less pronounced rhythm but greatly increases ethylene production in response to stress. We investigated what differences in ethylene synt
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4

Bender, Renar João, Eduardo Seibert, and Jeffrey K. Brecht. "Heat treatment effects on ACC oxidase activity of 'Keitt' mangoes." Brazilian Journal of Plant Physiology 15, no. 3 (2003): 145–48. http://dx.doi.org/10.1590/s1677-04202003000300003.

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With the use of ethylene dibromide for mango disinfestation being ruled out, vapor heat or hot water treatments are the only alternatives for quarantine treatments of mangoes. Physical treatments such as heat treatments have been implicated in higher incidence of physiological disorders and enhancement of ripening processes. Therefore, the objective of the present work was to determine the effects of hot water treatments on ethylene production and on the in vitro activity of ACC oxidase. Cv. Keitt mangoes were immersed for 3 min in hot water at 53 °C or 90 min in water at 46 °C. Immediately af
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5

Gorny, James R., and Adel A. Kader. "Elevated CO2 and/or Low O2 Atmospheres Influence ACC Synthase and ACC Oxidase during Long-term Storage of `Golden Delicious' Apple Fruit." HortScience 30, no. 4 (1995): 782C—782. http://dx.doi.org/10.21273/hortsci.30.4.782c.

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The objective of this study was to compare and contrast the mode of action by which elevated carbon dioxide and/or reduced oxygen atmospheres inhibit ethylene biosynthesis. `Golden Delicious' apple fruit were placed at 0C in one of the following four atmospheres: 1) air; 2) air + 5% CO2; 3) 2% O2 + 98% N2; or 4) 2% O2 + 5% CO2 + 93% N2 and then sampled monthly for 4 months. Ethylene biosynthesis rates and in vitro ACC synthase activities were closely correlated in all treatments. In vitro ACC synthase activity and ethylene biosynthesis rates were lowest in fruit treated with 5% CO2 + 2% O2, wh
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6

Gorny, James R., and Adel A. Kader. "699 PB 309 THE MODE OF CO2 ACTION ON ACC OXIDASE AND ITS ROLE IN INHIBITION OF ETHYLENE BIOSYNTHESIS." HortScience 29, no. 5 (1994): 533b—533. http://dx.doi.org/10.21273/hortsci.29.5.533b.

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Ethylene biosynthesis of Golden Delicious apple fruit at 20°C is rapidly inhibited by a controlled atmosphere of air + 20% CO2. However, in vitro ACC oxidase activity and ACC content were not significantly different between air and air + 20% CO2 treated fruit, To determine the in vivo effects of CO2 treatment, both in vivo and in vitro enzyme activity essays were performed in en atmosphere of air or air + 20% CO2. Western blots were also performed to quantify the amount of ACC oxidase protein present in the air and air + 20% CO2 treated fruit. We believe that in vivo cytosolic pH changes, indu
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7

Suh, Jung Nam, Kiyoshi Ohkawa, and Beyoung Hwa Kwack. "Senescence Symptoms after Emasculation Vary among Cymbidium Cultivars." HortScience 33, no. 4 (1998): 734–35. http://dx.doi.org/10.21273/hortsci.33.4.734.

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Response of cut Cymbidium flowers to emasculation was investigated. All Cymbidium cultivars that we examined produced ethylene during senescence. There were marked cultivar differences in the changes of fresh weight, ethylene production, and lip coloration after emasculation. In the case of `Venus', flower emasculation did not alter fresh weight, ethylene production, or lip coloration. There were differences in ACC oxidase in vivo activity among the three cultivars before and after emasculation. There was a correlation between the initial ACC oxidase activity and time to lip coloration in the
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8

Darvish, Mohammad, Habib Shirzad, Mohammadreza Asghari, et al. "24-Epibrasinolide Modulates the Vase Life of Lisianthus Cut Flowers by Modulating ACC Oxidase Enzyme Activity and Physiological Responses." Plants 10, no. 5 (2021): 995. http://dx.doi.org/10.3390/plants10050995.

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Ethylene is the most important factor playing roles in senescence and deterioration of harvested crops including cut flowers. Brassinosteroids (BRs), as natural phytohormones, have been reported to differently modulate ethylene production and related senescence processes in different crops. This study was carried out to determine the effects of different levels of 24-epibrassinolide (EBL) on ACC oxidase enzyme activity, the final enzyme in ethylene biosynthesis pathway, vase life, and senescence rate in lisianthus cut flowers. Harvested flowers were treated with EBL (at 0, 3, 6, and 9 µmol/L)
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9

Perkins-Veazie, P., J. K. Collins, and B. Cartwright. "Ethylene Production in Watermelon Fruit Varies with Cultivar and Fruit Tissue." HortScience 30, no. 4 (1995): 825G—826. http://dx.doi.org/10.21273/hortsci.30.4.825g.

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To determine whether ethylene production was related to locular tissue breakdown in watermelon, plugs from ripe `Jubilee', `Black Diamond', `Tiger Baby', `Mirage', and `King of Hearts' were taken from the skin (epidermis and hypoderm), rind (hypoderm and mesocarp), and placenta (locular and heart) tissues. ACC oxidase activity was <0.05 nmol·g–1·h–1 in locule and heart locations for all cultivars. Skin tissue had the highest activity, ranging from 0. 1 8 for `Jubilee' to 0.5 to 0.62 nmol·g–1·h–1 for the other four cultivars. ACC (1-aminocyclopropane-1-carboxylic acid) and ACC oxidase activi
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10

Perkins-Veazie, P., J. K. Collins, and B. Cartwright. "Ethylene Production in Watermelon Fruit Varies with Cultivar and Fruit Tissue." HortScience 30, no. 4 (1995): 825G—826. http://dx.doi.org/10.21273/hortsci.30.4.825.

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To determine whether ethylene production was related to locular tissue breakdown in watermelon, plugs from ripe `Jubilee', `Black Diamond', `Tiger Baby', `Mirage', and `King of Hearts' were taken from the skin (epidermis and hypoderm), rind (hypoderm and mesocarp), and placenta (locular and heart) tissues. ACC oxidase activity was <0.05 nmol·g–1·h–1 in locule and heart locations for all cultivars. Skin tissue had the highest activity, ranging from 0. 1 8 for `Jubilee' to 0.5 to 0.62 nmol·g–1·h–1 for the other four cultivars. ACC (1-aminocyclopropane-1-carboxylic acid) and ACC oxidase activi
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11

Pretel, M. T., M. Serrano, A. Amoros, F. Riquelme, and F. Romojaro. "Non-involvement of ACC and ACC oxidase activity in pepper fruit ripening." Postharvest Biology and Technology 5, no. 4 (1995): 295–302. http://dx.doi.org/10.1016/0925-5214(94)00025-n.

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12

Zhang, Z., C. J. Schofield, J. E. Baldwin, P. Thomas, and P. John. "Expression, purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from tomato in Escherichia coli." Biochemical Journal 307, no. 1 (1995): 77–85. http://dx.doi.org/10.1042/bj3070077.

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1-Aminocyclopropane-1-carboxylate (ACC) oxidase catalyses the final step in the biosynthesis of the plant hormone ethylene. The successful overexpression and characterization of active ACC oxidase from tomato has been achieved. PCR was used to insert the corrected cDNA coding for the tomato ACC oxidase into the pET-11a expression vector. Cloning of the resultant construct in Escherichia coli BL21(DE3)pLysE gave transformants which expressed ACC oxidase at levels greater than 30% of soluble protein under optimized conditions. When induced by addition of isopropyl-beta-D-thiogalactopyranoside (I
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13

Bhowmik, Pankaj Kumar, and Toshiyuki Matsui. "Changes in the Activity and Expression of 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase, ACC Oxidase, and Phenylalanine Ammonia-lyase in Asparagus Spears in Response to Wound-induced Ethylene Synthesis." HortScience 39, no. 5 (2004): 1074–78. http://dx.doi.org/10.21273/hortsci.39.5.1074.

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The molecular and enzymatic changes that follow harvesting of asparagus are important aspects for postharvest deterioration. To define the factors contributing to postharvest deterioration, the early changes in ethylene production and the activities and expression of 1-aminocyclopropane-1-carboxylate (ACC) synthase, ACC oxidase, and phenylalanine ammonia-lyase (PAL) were studied in whole spears and in excised top and bottom portions. As a result of wounding, ethylene production was found higher in both top and bottom portions compared with whole spears but followed the same trend reaching the
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14

Li, Yujie, Shaokang Liu, Jianxiong Hao, Huan Rao, Dandan Zhao, and Xueqiang Liu. "Antioxidant Benefits and Potential Mechanisms of Slightly Acidic Electrolyzed Water Germination in Sesame." Foods 12, no. 22 (2023): 4104. http://dx.doi.org/10.3390/foods12224104.

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Slightly acidic electrolytic water (SAEW) treatment for seed germination is a promising technique for sustainable agriculture. This study investigated the antioxidant activity of germinated sesame seeds treated with SAEW for the first time. Specifically, the impact and correlation of SAEW on the activities of total phenols, total flavonoids, and antioxidant oxidase in sesame seeds were examined. The results showed that SAEW with low ACC inhibited sesame germination, SAEW with high ACC promoted sesame germination, and sesame buds treated with SAEW with 30 mg/L and 50 mg/L ACC showed lower antio
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15

Muñoz, M. T., P. Aguado, N. Ortega, M. I. Escribano, and C. Merodio. "Regulation of ethylene and polyamine synthesis by elevated carbon dioxide in cherimoya fruit stored at ripening and chilling temperatures." Functional Plant Biology 26, no. 3 (1999): 201. http://dx.doi.org/10.1071/pp98115.

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In this study we focused on the effect of high CO2 level (20%) on ethylene and polyamine biosynthesis in cherimoya (Annona cherimola Mill.) fruits stored at ripening (20˚C) and chilling (6˚C) temperatures. At ripening temperature, CO2 inhibited ethylene production, but 1-aminocyclopropane-1- carboxylate (ACC) oxidase activity was similar to that in ripe control fruits. CO2 treatment led to a decline in putrescine (Put) and a major accumulation of spermidine (Spd) and spermine (Spm) without any effect on arginine decarboxylase (ADC) activity. These results confirm the preferential transformatio
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16

Gerasopoulos, D., and D. G. Richardson. "Storage-temperature-dependent Time Separation of Softening and Chlorophyll Loss from the Autocatalytic Ethylene Pathway and Other Ripening Events of `Anjou' Pears." Journal of the American Society for Horticultural Science 122, no. 5 (1997): 680–85. http://dx.doi.org/10.21273/jashs.122.5.680.

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Mature `Anjou' pears (Pyrus communis L.) continuously stored at 20 °C or -1 °C before transfer to 20 °C exhibited differences in the sequence of ripening events up to 100 days. Pears continuously held at 20 °C showed little change in ripening characteristics (chlorophyll, firmness, titratable acidity) for 14 to 28 days, then these characteristics decreased at a daily rate of 1.4% thereafter. A 40% increase in soluble polyuronides paralleled the firmness loss, while ACC did not exceed 0.5 nmol·g-1 until the 84th day, and internal ethylene did not exceed 0.2 μL·L-1 until after 90 days, whereas A
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17

Tian, M. S., C. G. Downs, R. E. Lill, and G. A. King. "A Role for Ethylene in the Yellowing of Broccoli after Harvest." Journal of the American Society for Horticultural Science 119, no. 2 (1994): 276–81. http://dx.doi.org/10.21273/jashs.119.2.276.

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Ethylene production from florets of `Shogun' harvested broccoli (Brassica oleracea L.var. italica) held at 20C in darkness increased as the sepal tissues yellowed. The pattern of respiration rate and ethylene production from branchlets or entire heads was similar, although the magnitude of ethylene and carbon dioxide production appeared to be diluted by the other fleshy stem tissues. The reproductive structures, stamens and pistil, may have a role in determining the rate of sepal degreening, since removing them from florets reduced the yellowing rate. The pistil and stamens also had 7-fold hig
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18

Rothan, C., and J. Nicolas. "Changes in Acidic and Basic Peroxidase Activities during Tomato Fruit Ripening." HortScience 24, no. 2 (1989): 340–42. http://dx.doi.org/10.21273/hortsci.24.2.340.

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Abstract Activities of the acidic and basic peroxidases from tomato fruit (Lycopersicon esculentum Mill. cv. Flora Dade) were determined at six ripening stages, from green to red-ripe fruits. Both the acidic and basic peroxidases reached a maximum during the climacteric, at the pink stage, but the relative increase in basic peroxidase activity was much more pronounced. Changes in the peroxidase, IAA oxidase, and ACC oxidase activities of the basic peroxidases paralleled the changes in ethylene production. However, in the presence of calcium, the degree of activation of peroxidase was constant
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19

López-Gómez, Antonio, Alejandra Navarro-Martínez, Alberto Garre, Asunción Iguaz, and Ginés Benito Martínez-Hernández. "The Potential of Essential Oils from Active Packaging to Reduce Ethylene Biosynthesis in Plant Products. Part 2: Fruits (Blueberries and Blackberries)." Plants 12, no. 19 (2023): 3418. http://dx.doi.org/10.3390/plants12193418.

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Plant essential oils (EOs) have an important ability to inhibit ethylene biosynthesis. Nevertheless, the effects of EOs on the key components of ethylene biosynthesis (l-aminocyclopropane-1-carboxylic (ACC) oxidase activity, ACC synthase activity, and ACC content) have not yet been thoroughly studied. Accordingly, this study focused on the effects of emitted EOs from active packaging (EO doses from 100 to 1000 mg m−2) on the key components of ethylene biosynthesis of blueberries and blackberries under several storage temperatures. Anise EO and lemon EO active packaging induced the greatest inh
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20

Di, Qinghua, Yansu Li, Shuzhen Li, et al. "Photosynthesis Mediated by RBOH-Dependent Signaling Is Essential for Cold Stress Memory." Antioxidants 11, no. 5 (2022): 969. http://dx.doi.org/10.3390/antiox11050969.

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Cold tolerance is improved by cold stress acclimation (CS-ACC), and the cold tolerance level is ‘remembered’ by plants. However, the underlying signaling mechanisms remain largely unknown. Here, the CS memory mechanism was studied by bioinformation, plant physiological and photosynthetic parameters, and gene expression. We found that CS-ACC induced the acquisition of CS memory and enhanced the maintenance of acquired cold tolerance (MACT) in cucumber seedlings. The H2O2 content and NADPH oxidase activity encoded by CsRBOH was maintained at higher levels during recovery after CS-ACC and inhibit
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21

López-Gómez, Antonio, Alejandra Navarro-Martínez, Alberto Garre, Francisco Artés-Hernández, Pedro Villalba, and Ginés Benito Martínez-Hernández. "The Potential of Essential Oils from Active Packaging to Reduce Ethylene Biosynthesis in Plant Products. Part 1: Vegetables (Broccoli and Tomato)." Plants 12, no. 19 (2023): 3404. http://dx.doi.org/10.3390/plants12193404.

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Essential oils (EOs) extracted from plants have a high potential to reduce ethylene biosynthesis, although their effects have not been deeply studied yet on the key components of the ethylene biosynthesis pathway: l-aminocyclopropane-1-carboxylic (ACC) oxidase activity, ACC synthase activity, and ACC content. Hence, the present study aimed to elucidate the effects of released EOs from active packaging (with different EO doses ranging from 100 to 1000 mg m−2) on the ethylene biosynthesis key components of broccoli and tomato under different storage temperature scenarios. The largest ethylene in
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22

Gorny, James R., and Adel A. Kader. "Low Oxygen and Elevated Carbon Dioxide Atmospheres Inhibit Ethylene Biosynthesis in Preclimacteric and Climacteric Apple Fruit." Journal of the American Society for Horticultural Science 122, no. 4 (1997): 542–46. http://dx.doi.org/10.21273/jashs.122.4.542.

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Autocatalytic C2H4 biosynthesis in preclimacteric apple fruit (Malus domestica Borkh. `Golden Delicious') was prevented by storage in atmospheres of 20% CO2-enriched air (17% O2 + 63% N2) or 0.25% O2 (balance N2). In preclimacteric fruit, both treatments inhibited C2H2 biosynthesis by suppressing expression of ACC synthase (ACC-S) at the mRNA level. ACC oxidase (ACC-O) mRNA abundance and in vitro enzyme activity also were impaired by these treatments. However, the conversion of ACC to C2H4 never became the rate limiting step in C2H4 biosynthesis. C2H4 biosynthesis also was effectively inhibite
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23

Li, Ling, Hai Xue Liu, Yong Bo Peng, Shi Li, and Tie Ling Liu. "Effects of Exogenous Ethylene on AC and Rin Tomato Fruit." Advanced Materials Research 1033-1034 (October 2014): 677–80. http://dx.doi.org/10.4028/www.scientific.net/amr.1033-1034.677.

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The flesh firmness of AC andrinmutant tomato fruits picked freshly were the largest. Respiration rate and ethylene production were very low at this time. With ethylene production increase, fruit firmness began to decline. 100μL/L ethephon significantly increased AC tomato fruit ethylene release, respiration rate, ACS activity and ACO activity, and decreased flesh firmness. However, there were no significant differences inrinmutant between control and ethephon treatment. It was shown RIN transcription factor regulated ethylene biosynthesis by ACC synthase and ACC oxidase.
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24

Bender, Renar João, Jeffrey Karl Brecht, and Steven Alonzo Sargent. "Reduced ethylene synthesis of mangoes under high CO2 atmosphere storage." Acta Scientiarum. Agronomy 43 (March 18, 2021): e51540. http://dx.doi.org/10.4025/actasciagron.v43i1.51540.

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. High CO2 atmospheres have been reported to be accountable for slower ripening processes of many fruit species. In modified or controlled atmosphere storage of mangoes (Mangifera indica L.) delayed ripening is attributed to the effects of CO2 on ethylene biosynthesis, which is reduced under CO2 concentrations beyond 10%. In the present work the objective was to determine if those elevated CO2 atmospheres on ethylene synthesis could be attributed to the action of CO2 upon ACC oxidase. Mature green or tree ripe `Tommy Atkins` mangoes were, in four experiments, held in a flow through system of e
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25

Pech, Jean-Claude. "Postharvest Behavior of Transgenic Cantaloupe Melons with Suppressed ACC Oxidase Activity." HortScience 31, no. 4 (1996): 571d—571. http://dx.doi.org/10.21273/hortsci.31.4.571d.

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We have generated transgenic Cantaloupe Charentais melons expressing an ACC oxidase antisense gene in which ethylene production was reduced to less than 1% as compared to control untransformed fruits. As a consequence, some aspects of the ripening process were strongly inhibited (aroma volatiles production, chlorophyll and cell wall degradation, pigmentation of the rind, activation of peduncular abscission zone) while others remained unchanged (coloration of the flesh), allowing us to distinguish between ethylene-dependent and ethylene-independent pathways. Some postharvest characteristics of
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26

Vioque, B., and J. M. Castellano. "'BLANQUILLA' PEAR ACC OXIDASE. EFFECT OF CO2 ON IN VIVO ACTIVITY." Acta Horticulturae, no. 800 (October 2008): 1069–76. http://dx.doi.org/10.17660/actahortic.2008.800.146.

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27

Terai, Hirofumi, Hironobu Tsuchida, Masashi Mizuno, and Noriyoshi Matsui. "Influence of Short-term Treatment with High CO2 and N2 on Ethylene Biosynthesis in Tomato Fruit." HortScience 33, no. 1 (1998): 103–4. http://dx.doi.org/10.21273/hortsci.33.1.103.

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Tomato fruit were given a short-term (24 h) high CO2 (80%) or N2 (100%) treatment and then transferred to air storage at 20 °C. The CO2 treatment stimulated ACC oxidase activity and ethylene production, whereas the N2 treatment increased ACC content but did not increase ethylene production. Both CO2, and N2 treatments delayed ripening for one day, but fruit ripened normally. Although short-term 80% CO2, had a stimulating effect, and 100 % N2 had no effect on ethylene production, ripening was delayed slightly by both treatments. Chemical name used: 1-aminocyclopropane-1-carboxylic acid (ACC).
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28

Terai, Hirofumi, Hironobu Tsuchida, Masashi Mizuno, and Noriyoshi Matsui. "Influence of Short-term Treatment with High CO2 and N2 on Ethylene Biosynthesis in Tomato Fruit." HortScience 33, no. 1 (1998): 103–4. http://dx.doi.org/10.21273/hortsci.33.1.0103.

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Tomato fruit were given a short-term (24 h) high CO2 (80%) or N2 (100%) treatment and then transferred to air storage at 20 °C. The CO2 treatment stimulated ACC oxidase activity and ethylene production, whereas the N2 treatment increased ACC content but did not increase ethylene production. Both CO2, and N2 treatments delayed ripening for one day, but fruit ripened normally. Although short-term 80% CO2, had a stimulating effect, and 100 % N2 had no effect on ethylene production, ripening was delayed slightly by both treatments. Chemical name used: 1-aminocyclopropane-1-carboxylic acid (ACC).
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29

Czapski, Janusz, Artur Miszczak, and Marian Saniewski. "The Effect of MethyI Jasmonate on Ethylene Production, ACC Oxidase Activity and Carbon Dioxide Evolution in the Yellowish-Tangerine Tomato Fruits (Lycopersicon esculentum Mill.)." Acta Agrobotanica 49, no. 1-2 (2013): 67–72. http://dx.doi.org/10.5586/aa.1996.006.

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The yellowish-tangerine tomato (cv. Bursztyn) in the green, light yellow and yellow stages of ripening were treated with 0.1% and 1.0% of methyl jasmonate (JA-Me) in lanolin paste and kept for several days and then they were evaluated for production of ethylene, ACC oxidase activity and CO<sub>2</sub> evolution. Production of endogenous ethylene in mature green fruits was low and increased during ripening. JA-Me stimulated ethylene production and ACC oxidase activity in all investigated stages of fruit ripening. Slices excised from mature green fruits produced highest amount of car
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30

Larrigaudiere, C., I. Recasens, J. Graell, and M. Vendrell. "Harvest maturity related changes in the cold-induced activation of 1-aminocyclopropane-1-carboxylic acid metabolism in Granny Smith apples / Efecto del estado de madurez sobre la activación por frío del metabolismo del ácido 1-aminociclopropano-1-carboxílico en manzanas Granny Smith." Food Science and Technology International 5, no. 3 (1999): 223–28. http://dx.doi.org/10.1177/108201329900500304.

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Changes in 1-aminocyclopropane-1-carboxylic acid metabolism in apples ( Malus domestica Borkh cv Granny Smith) were studied in relation to cold storage. Emphasis was given to the differential re sponsiveness of fruits to cold treatment as a function of stage of maturity at harvest. Fruits were held at 1 or 20 °C for 30 days, respectively, or exposed to 1 °C for 10 days and then storaged at 20 °C for up to 30 days. Fruits at 20 °C showed typical climacteric behavior. Differences at 1 °C between maturity stages in ethylene production and ACC oxidase activity were abolished, which showed that col
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31

Koslanund, Rumphan, Douglas D. Archbold, and Kirk W. Pomper. "Pawpaw [Asimina triloba (L.) Dunal] Fruit Ripening. I. Ethylene Biosynthesis and Production." Journal of the American Society for Horticultural Science 130, no. 4 (2005): 638–42. http://dx.doi.org/10.21273/jashs.130.4.638.

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Pawpaw fruit ethylene production, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO) activities, and tissue content of the ethylene precursor ACC and conjugate malonyl-ACC (MACC) were measured during postharvest ripening. Fruit were harvested near the advent of the ripening process and were ripened at room temperature. The fruit displayed increases in ethylene production and respiration rate during ripening with maxima for both 3 days after harvest. Mean ethylene maxima on a fresh weight basis were 4.7 and 7.6 μg·kg-1·h-1 and mean respiratory (CO2 production) maxi
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32

Ribeiro, Dimas M., Ana M. Mapeli, Marcelo A. G. Carnelossi, Carla A. Delatorre, and Raimundo S. Barros. "Dormancy breakage of Stylosanthes humilis seeds by aluminium." Seed Science Research 20, no. 3 (2010): 145–52. http://dx.doi.org/10.1017/s0960258510000164.

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AbstractPhysiological dormancy of scarified seeds of Townsville stylo (Stylosanthes humilis HBK) was released by acidic aluminium (Al3+) solution. Antiethylenic substances inhibited germination of low-pH-stimulated dormant seeds, with a correspondingly low ethylene production and low activity of 1-aminocyclopropane-1-carboxylate (ACC) oxidase in seeds. On the other hand, antiethylenic substances did not decrease the germination of Al3+-stimulated seeds, but ACC oxidase activity and ethylene production by the seeds was decreased to a large extent. These data provide evidence that dormancy break
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33

Tovar, Beatrı́z, Hugo S. Garcı́a, and Miguel Mata. "Physiology of pre-cut mango. I. ACC and ACC oxidase activity of slices subjected to osmotic dehydration." Food Research International 34, no. 2-3 (2001): 207–15. http://dx.doi.org/10.1016/s0963-9969(00)00154-x.

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34

Ghattas, Wadih, Michel Giorgi, Christian Gaudin, Antal Rockenbauer, Marius Réglier, and A. Jalila Simaan. "Characterization of Cu(II)-ACC Complexes and Conversion of the Bound ACC into Ethylene in the Presence of Hydrogen Peroxide. Detection of a Brown Intermediate at Low Temperature." Bioinorganic Chemistry and Applications 2007 (2007): 1–9. http://dx.doi.org/10.1155/2007/43424.

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Two copper(II)-ACC complexes were prepared and characterized: [Cu(bpy)(ACC)(H2O)]⋅CO4(1) and[Cu(ACC)2]3⋅4H2O(2). Their crystallographic structures are described and analyzed. Spectroscopic characterizations (UV-visible and EPR) confirm that the structure is maintained in solution. These complexes are able to produce ethylene in the presence of hydrogen peroxide in an “ACC Oxidase-like” reaction in water and in methanol. The conversion of ACC into ethylene depends on the amount of base, and, in methanol, 3 equivalents of NaOH are needed for optimum activity. The base is proposed to play a role
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35

Chojnowski, Mariusz, and Anna Skorupińska. "In vivo and in vitro Activity of 1-aminocyclopropane-1-carboxylic Acid Oxidase in Germinating Seeds of China Aster (Callistephus chinensis Nees)." Journal of Horticultural Research 28, no. 2 (2020): 11–20. http://dx.doi.org/10.2478/johr-2020-0021.

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Abstract The activity of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO; EC 1.4.3.3) in germinating seeds of Callistephus chinensis was studied. For maximum recovery of ACO activity in vitro, the presence of 10% (w/v) insoluble polyvinylpolypyrrolidone (PVPP) and 30% of glycerol in the extraction medium was necessary. The optimum pH for this activity was 7.0. Ethylene production by whole achenes or enzymatic extract increased due to increasing 1-aminocyclopropane-1-carboxylic acid (ACC) concentrations. Saturation level of ACC for in vivo ACO activity was 10−1 M and Vmax was 10.89 nL C2H4·m
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36

Chojnowski, Mariusz, and Anna Skorupińska. "In vivo and in vitro Activity of 1-aminocyclopropane-1-carboxylic Acid Oxidase in Germinating Seeds of China Aster (Callistephus chinensis Nees)." Journal of Horticultural Research 28, no. 2 (2020): 11–20. http://dx.doi.org/10.2478/johr-2020-0021.

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AbstractThe activity of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO; EC 1.4.3.3) in germinating seeds of Callistephus chinensis was studied. For maximum recovery of ACO activity in vitro, the presence of 10% (w/v) insoluble polyvinylpolypyrrolidone (PVPP) and 30% of glycerol in the extraction medium was necessary. The optimum pH for this activity was 7.0. Ethylene production by whole achenes or enzymatic extract increased due to increasing 1-aminocyclopropane-1-carboxylic acid (ACC) concentrations. Saturation level of ACC for in vivo ACO activity was 10−1 M and Vmax was 10.89 nL C2H4·mg
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37

Finlayson, Scott A., David M. Reid, and Page W. Morgan. "Root and leaf specific ACC oxidase activity in corn and sunflower seedlings." Phytochemistry 45, no. 5 (1997): 869–77. http://dx.doi.org/10.1016/s0031-9422(97)00080-0.

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38

Hong, Ji Heun, A. Keith Cowan, and Seung Koo Lee. "Glucose Inhibits ACC Oxidase Activity and Ethylene Biosynthesis in Ripening Tomato Fruit." Plant Growth Regulation 43, no. 1 (2004): 81–87. http://dx.doi.org/10.1023/b:grow.0000038248.54232.6a.

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39

Gorny, James R., and Adel A. Kader. "Controlled-atmosphere Suppression of ACC Synthase and ACC Oxidase in `Golden Delicious' Apples during Long-term Cold Storage." Journal of the American Society for Horticultural Science 121, no. 4 (1996): 751–55. http://dx.doi.org/10.21273/jashs.121.4.751.

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Preclimacteric `Golden Delicious' apples (Malus domestica Borkh.) were stored at 0 °C in: air; air + 5% CO2; 2% O2 + 98% N2; or 2% O2 + 5% CO2 + 93% N2, and sampled monthly for 4 months to investigate the mechanism(s) by which reduced O2 and/or elevated CO2 atmospheres inhibit C2H4 biosynthesis. Ethylene biosynthesis rates and in vitro ACS activity were closely correlated in all treatments, while in vitro ACO activity significantly increased over time regardless of the treatment. Only a small amount of C2H4 biosynthesis inhibition by lowered O2 and/or elevated CO2 atmospheres could be accounte
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40

Wawrzyńczak, A., Z. B. Jóźwiak, and K. P. Rutkowski. "FRUIT QUALITY AND ACC OXIDASE ACTIVITY IN 'RUBINSTAR' APPLES TREATED WITH 1-MCP." Acta Horticulturae, no. 796 (August 2008): 147–53. http://dx.doi.org/10.17660/actahortic.2008.796.17.

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41

Gallardo, M., M. del Carmen Gómez-Jiménez, and A. Matilla. "Involvement of calcium in ACC-oxidase activity from Cicer arietinum seed embryonic axes." Phytochemistry 50, no. 3 (1999): 373–76. http://dx.doi.org/10.1016/s0031-9422(98)00591-3.

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42

Zia Ul Haq, Muhammad, Zheng Zhang, Jiajia Wei, and Sheng Qiang. "Ethylene Biosynthesis Inhibition Combined with Cyanide Degradation Confer Resistance to Quinclorac in Echinochloa crus-galli var. mitis." International Journal of Molecular Sciences 21, no. 5 (2020): 1573. http://dx.doi.org/10.3390/ijms21051573.

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Echinochloa crus-galli var. mitis has rarely been reported for herbicide resistance, and no case of quinclorac resistance has been reported so far. Synthetic auxin-type herbicide quinclorac is used extensively to control rice weeds worldwide. A long history of using quinclorac in Chinese rice fields escalated the resistance in E. crus-galli var. mitis against this herbicide. Bioassays in Petri plates and pots exhibited four biotypes that evolved into resistance to quinclorac ranking as JS01-R > AH01-R > JS02-R > JX01-R from three provinces of China. Ethylene production in these biotyp
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43

Yu, Hui, Lei Qin, Hai Hu, and Zhanli Wang. "Alteration of the Gut Microbiota and Its Effect on AMPK/NADPH Oxidase Signaling Pathway in 2K1C Rats." BioMed Research International 2019 (May 22, 2019): 1–8. http://dx.doi.org/10.1155/2019/8250619.

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Background. The purpose of this study was to evaluate the alteration of the gut microbiota and its effect on adenosine monophosphate-activated protein kinase (AMPK)/nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase) signaling pathway in two-kidney one-clip (2K1C) rats. Methods. The 2K1C rat models were established. The rats were randomly divided into the following 2 groups: 2K1C group and sham group. Alterations of the gut microbiota were analyzed based on the high throughput sequencing method. Plasma concentrations of short chain fatty acids (SCFAs) were measured by chromatog
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44

Zafari, Somaieh, Greg C. Vanlerberghe, and Abir U. Igamberdiev. "The Role of Alternative Oxidase in the Interplay between Nitric Oxide, Reactive Oxygen Species, and Ethylene in Tobacco (Nicotiana tabacum L.) Plants Incubated under Normoxic and Hypoxic Conditions." International Journal of Molecular Sciences 23, no. 13 (2022): 7153. http://dx.doi.org/10.3390/ijms23137153.

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The transgenic tobacco (Nicotiana tabacum L.) plants with the modified levels of alternative oxidase (AOX) were used to evaluate the physiological roles of AOX in regulating nitro-oxidative stress and metabolic changes after exposing plants to hypoxia for 6 h. Under normoxia, AOX expression resulted in the decrease of nitric oxide (NO) levels and of the rate of protein S-nitrosylation, while under hypoxia, AOX overexpressors exhibited higher NO and S-nitrosylation levels than knockdowns. AOX expression was essential in avoiding hypoxia-induced superoxide and H2O2 levels, and this was achieved
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45

Zhernakov, Alexander I., Viktor E. Tsyganov, Aleksey U. Borisov, and Igor A. Tikhonovich. "THE PEA GENE Crt CONTROLING THE MORPHOGENETIC RESPONSE OF THE ROOT IS INVOLVED IN REGULATION OF ACC-OXIDASE ACTIVITY." Ecological genetics 10, no. 1 (2012): 62–73. http://dx.doi.org/10.17816/ecogen10162-73.

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The ethylene status of the pea mutant SGEcrt with altered morphology of the root system, whose development is dramatically dependent on the mechanical conditions of the environment, was studied. The role of ethylene in phenotypic manifestation of mutant root system was confirmed. It was shown that the mutant is characterized by increased production of and increased sensitivity to ethylene and by changes in regulation of ethylene biosynthesis leading to increased activity of the ethylene-producing enzyme ACC-oxidase.
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46

Finlayson, Scott A., and David M. Reid. "Influence of CO2 on ACC oxidase activity from roots of sunflower (Helianthus annuus) seedlings." Phytochemistry 35, no. 4 (1994): 847–51. http://dx.doi.org/10.1016/s0031-9422(00)90624-1.

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47

Guillén, P., A. Domènech, C. Larrigaudère, and M. Vendrell. "Ethylene-induced rise of abscisic acid levels and ACC oxidase activity in immature melons." Journal of Horticultural Science and Biotechnology 73, no. 3 (1998): 313–16. http://dx.doi.org/10.1080/14620316.1998.11510979.

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48

Wang, Nan, Huixin Fang, Qingxi Yang, Zhiyong Liu, Hui Feng, and Shujuan Ji. "Exogenous Melatonin Alleviated Leaf Yellowing via Inhibiting Respiration and Ethylene Biosynthesis during Shelf Life in Pakchoi." Plants 11, no. 16 (2022): 2102. http://dx.doi.org/10.3390/plants11162102.

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Postharvest yellowing of leafy plant is a manifestation of senescence, and melatonin (MT) is known to delay leaf senescence in some higher plants. Herein, we investigated the effect of exogenous MT treatment on postharvest pakchoi by monitoring the ethylene biosynthesis and respiratory metabolism. Results showed that exogenous MT effectively extended the shelf life, delayed leaf yellowing, minimized the alteration in Fv/Fm ratio and maintained higher integrity of chloroplast in pakchoi. There was a significant correlation between yellowing index, respiration rate and ethylene production. MT tr
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49

Chojnowski, M., F. Corbineau, and D. Côme. "Physiological and biochemical changes induced in sunflower seeds by osmopriming and subsequent drying, storage and aging." Seed Science Research 7, no. 4 (1997): 323–32. http://dx.doi.org/10.1017/s096025850000372x.

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AbstractSunflower (Helianthus annuus L.) seeds show more germination at high temperatures (25–30°C) than at temperatures below 20°C. Osmopriming with polyethylene glycol-6000 for 3–5 days at 15°C strongly increases germination at suboptimal temperatures. This stimulatory effect of priming persists after seed redrying and during subsequent storage at 20°C (55% RH) for at least 14 weeks. However, primed seeds deteriorate faster than untreated seeds during accelerated aging (45°C, 100% RH). The longer the priming treatment, the higher is the amount of germination but at the same time the higher i
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50

Lagunes, L., B. Tovar, M. Mata, J. C. Vinay-Vadillo, J. De La Cruz, and H. S. Garcia. "Effect of Exogenous Ethylene on ACC Content and ACC Oxidase Activity During Ripening of Manila Mangoes Subjected to Hot Water Treatment." Plant Foods for Human Nutrition 62, no. 4 (2007): 157–63. http://dx.doi.org/10.1007/s11130-007-0057-5.

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