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Journal articles on the topic 'Acid fraction'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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1

Takai, A., M. Troschka, G. Mieskes, and A. V. Somlyo. "Protein phosphatase composition in the smooth muscle of guinea-pig ileum studied with okadaic acid and inhibitor 2." Biochemical Journal 262, no. 2 (1989): 617–23. http://dx.doi.org/10.1042/bj2620617.

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Using okadaic acid, a potent inhibitor of type 2A and type 1 protein phosphatases, and inhibitor 2, an intrinsic inhibitory factor of type 1 phosphatase, we characterized the phosphorylated myosin light-chain (PMLC) phosphatase activity in the smooth-muscle extracts of guinea-pig ileum. In the intact fibres the control activity was 254 +/- 13 nmol of Pi/min per g wet wt. (n = 15) against 32P-labelled PMLC (4 microM) from chicken gizzard. The following phosphatase fractions were identified: an inhibitor-2-sensitive (type 1) fraction (fractional activity = 35%), a Mg2+-dependent and okadaic acid

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2

Vogt, J. A., A. J. Fischman, M. Kempf, Y. M. Yu, R. G. Tompkins, and J. F. Burke. "A general model for analysis of the tricarboxylic acid cycle with use of [13C]glutamate isotopomer measurements." American Journal of Physiology-Endocrinology and Metabolism 266, no. 6 (1994): E1012—E1022. http://dx.doi.org/10.1152/ajpendo.1994.266.6.e1012.

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A generalized steady-state model was developed for determining tricarboxylic acid cycle fractional fluxes from 13C nuclear magnetic resonance (NMR) data. The model relates the measured mole fractions of [13C]glutamate isotopomers to the fractional fluxes and predicted mole fractions of isotopomers of oxaloacetate (OAA) and acetyl-CoA. This model includes cycling between OAA and fumarate. Fractional fluxes are determined by fitting the model equations to NMR parameters by use of nonlinear least squares. Although only fractional fluxes can be determined from 13C-NMR data, when they are combined

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Pawar, Reshma, and A. Balasubramaniam. "Densitometric HPTLC analysis of the Acacia catechu wild fractions for phenolics." International Journal of Experimental Research and Review 32 (August 30, 2023): 260–69. http://dx.doi.org/10.52756/ijerr.2023.v32.022.

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Traditional "Ayurvedic" medicine from India has traditionally used Acacia catechu. The herbal extract is the primary component, although there have been no attempts to standardize it as an active agent or marker. A chromatographic fingerprint represents the chemical components of herbal remedies that are therapeutically advantageous. This study suggests utilizing an HPTLC approach to assess phenols like protocatechuic acid and gallic acid in Acacia catechu extract fractions made of water, ethyl acetate, and butanol. According to the study, protocatechuic acid (11.85%) in the water fraction of

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Suina, Irina Olegovna, Inna Ivanovna Terninko, Yuliya Eduardovna Generalova, Yelena Vladimirovna Burtseva, and Yelizaveta Sergeyevna Bazanova. "STUDY OF SEPARATE FRACTIONS OF ARISTOLOCHIA CLEMATITIS L. HERB FOR THE PRESENCE OF DIFFER-ENT GROUPS OF BAS." chemistry of plant raw material, no. 2 (June 10, 2020): 197–207. http://dx.doi.org/10.14258/jcprm.2020026462.

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The objective of the work was generation of Aristolochia clematitis L. herb fractions and their further study for the presence of different groups of biologically active substances (including aristolochic acids), which was achieved by addressing the following tasks: obtaining fractions from A. clematitis L. herb, preliminary TLC test, HPLC fraction analysis. Fractionation scheme for A. clematitis L. herb extracts was proposed. 4 fractions with different distribution of biologically active substances (chloroform, butanol, ethylacetate and water) were obtained. Using the TLC method and HPLC anal

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Górski, Piotr M., Marian Jurzysta, Stanisław Burda, Wiesław A. Oleszek, and Michał Płoszyński. "Studies on Medicago lupulina saponins. 2. Isolation, chemical characterization and biological activity of saponins from M. lupulina tops." Acta Societatis Botanicorum Poloniae 53, no. 4 (2014): 527–33. http://dx.doi.org/10.5586/asbp.1984.048.

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Two pure saponin fractions from the tops of <em>M. lupulina</em> were isolated and characterized. The fractions named Ma and Ss varied in composition and biological activity. It was shown by TLC that fraction Ma contained 5 components. In the acid hydrolysates of that fraction only one aglycone (medicagenic acid) and 4 sugars: rhamnose, xylose, arabinose, glucuronic acid were found. The Ss fraction contained 11 components. In its acid hydrolysates, soyasapogenols B, C, D, E, F and two new aglycones N and An were found. The same sugars as above and additionally glucose and galactose

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6

Yakubu, Ojochenemi E., Olawale Otitoju, Janya Danjuma, Dulrashid Dauda Maianguwa, Isaac John Umaru, and Gloria Omonefe Oladele. "The Comparative Suitability of Different Solvents Used in the Fractionation of Methanol Leaf Extract of Persea americana Mill: An in Vitro Studies." African Journal of Biochemistry and Molecular Biology Research 1, no. 1 (2024): 547–68. https://doi.org/10.58578/ajbmbr.v1i1.3664.

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This research investigated the suitability of various solvents used in the fractionation of methanol leaf extract of P. americana to ascertain the flavonoid, vitamin and amino acid profile of the fractions. It has been demonstrated that a number of Persea americana plant components contain different phytochemicals associated with important biological function. Fresh leaves of P. americana were harvested from Baissa in Taraba State. The plant materials were air dried under shade at ambient temperature. The dried plant materials were pulverized to fine powder using mortar and pestle. The pulveri

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7

Ru, Pang, Gan, Liu, and Bao. "Phenolic Compounds and Antioxidant Activities of Potato Cultivars with White, Yellow, Red and Purple Flesh." Antioxidants 8, no. 10 (2019): 419. http://dx.doi.org/10.3390/antiox8100419.

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The contents of total phenolics (TPC), individual phenolic acid and antioxidant activities in the free and bound fractions of potato with different flesh colors were systematically investigated. The TPC and antioxidant capacity in the bound fraction was significantly lower than that in the free fraction. Chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, caffeic acid, p-coumaric acid and ferulic acid were detected in the free fraction with chlorogenic acid being the most predominant, accounting for 35.21–81.78% of the total content. Caffeic acid, p-coumaric acid and ferulic acid we

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Wiyono, Bambang, Sanro Tachibana, and Tinambunan Djaban. "CHEMICAL COMPOSITIONS OF PINE RESIN, ROSIN AND TURPENTINE OIL FROM WEST JAVA." JOURNAL OF FORESTRY RESEARCH 3, no. 1 (2006): 7–17. https://doi.org/10.20886/ijfr.2006.3.1.7-17.

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  This study was conducted to identify chemical composition of merkus pine resin, rosin and turpentine oil. Initially, pine resin was separated into neutral and acidic fractions with an aqueous 4% sodium hydroxide solution. After methylation, the fraction containing turpentine oil and rosin were analyzed by gas chromatography (GC), and gas chromatograph mass spectrometry (GC-MS), respectively. The neutral fraction of pine resin and turpentine oil mainly consisted of a-pinene, D-3-carene and b-pinene. Based on mass spectral comparison, the major constituents of the acidic fraction and rosi

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9

Karamać, M., E. Sendrejová, A. Kosińska, and D. Urminská. "Presence of ferulic acid in wheat glutenin fraction and its enzymatic hydrolysates – a short report." Czech Journal of Food Sciences 25, No. 6 (2008): 359–64. http://dx.doi.org/10.17221/751-cjfs.

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Three protein fractions: albumin-globulin, gliadin, and glutenin were extracted from wheat grains. Enzymatic hydrolysates were obtained from each fraction using trypsin, protease from <I>Bacillus subtilis</I> and Subtilisin Carlsberg. Ferulic acid was detected neither in albumin-globulin and gliadin fractions nor in their hydrolysates. The RP-HPLC and SE-HPLC chromatograms of the glutenin fraction and its hydrolysates revealed the presence of peaks originated from ferulic acid and ferulic acid derivative/complex. The content of ferulic acid in the glutenin fraction was 1.12 mg/g. D

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Nakasone, S., T. Ohshita та T. Iwamasa. "Heterogeneity of pig lysosomal acid α-glucosidase. Affinity to Sephacryl S-200 gel and tissue distribution". Biochemical Journal 279, № 3 (1991): 719–26. http://dx.doi.org/10.1042/bj2790719.

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Acid alpha-glucosidase purified from pig liver showed heterogeneity in its affinity to Sephacryl S-200 gel. Acid alpha-glucosidase was separated into two fractions (S1 and S2) by Sephacryl S-200 affinity chromatography. Each fraction contained components at apparent 76 kDa and 67 kDa on SDS/PAGE. The amount of S1 fraction was about 1.3 times that of the S2 fraction. In the kidney the ratio of S1 to S2 fraction was similar to that in the liver. However, the heart contained 1.3 times as much S2 fraction as S1 fraction. The spleen acid alpha-glucosidase consisted mainly of S1 fraction, containing

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Qamar, Kehkashan Arshad, Ahsana Dar Farooq, Bina S. Siddiqui, Nurul Kabir, and Sabira Begum. "Antiproliferative Effects of Ocimum basilicum Methanolic Extract and Fractions, Oleanolic Acid and 3-epi-Ursolic Acid." Current Traditional Medicine 6, no. 2 (2020): 134–46. http://dx.doi.org/10.2174/2215083805666191010152439.

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Aims: The aim of the current study was to identify active compound(s) responsible for the antiproliferative effects of O. basilicum and explore their underlying mechanism/s. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in

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12

Mustarichie, Resmi. "THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)." Asian Journal of Pharmaceutical and Clinical Research 10, no. 2 (2017): 343. http://dx.doi.org/10.22159/ajpcr.2017.v10i2.15724.

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Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spe

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13

Ghosh, S. K., S. Majumder, N. K. Mukhopadhyay, and S. K. Bose. "Role of ATP and enzyme-bound nascent peptides in the control of elongation for mycobacillin synthesis." Biochemical Journal 240, no. 1 (1986): 265–68. http://dx.doi.org/10.1042/bj2400265.

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The enzyme fraction A, a constituent of the three-fraction (A, B and C) enzyme complex mycobacillin synthetase, elongated tri- and tetra-peptides, under enzyme-bound conditions, to tetra- and penta-peptides respectively in the presence of the ‘next’ amino acid (in the mycobacillin sequence). The enzyme fraction B synthesized hexapeptide from free pentapeptide and the next amino acid, but synthesized heptapeptide from hexapeptide only under enzyme-bound conditions in the presence of the next amino acid. Similarly, the enzyme fraction C synthesized decapeptide from free nonapeptide in the presen

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14

Sahib, Lasna, K. Ally, K. S. Ajith, Biju Chacko, G. Radhika, and K. S. Anil. "Evaluation of chemical composition, fibre fractions and carbohydrate and protein partitioning of paddy straw, Guinea grass, and hybrid Napier (CO5)." Journal of Veterinary and Animal Sciences 56, no. 2 (2025): 268–73. https://doi.org/10.51966/jvas.2025.56.2.268-273.

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The nutritional evaluation of fodder resources is essential for optimising ruminant feeding strategies. This study assessed the chemical composition, fibre fractions and CNCPS (Cornell Net Carbohydrate and Protein System) carbohydrate and protein partitioning of paddy straw, Guinea grass and Hybrid Napier (CO5). Four samples of each fodder were analysed for dry matter, crude protein, ether extract, ash, neutral detergent fibre, acid detergent fibre, lignin, starch, buffer soluble protein, non-protein nitrogen, neutral detergent insoluble protein, and acid detergent insoluble protein. Carbohydr

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15

Abdujalolovna, Siddiqova Gulchehra, Musulmonov Noryigit Hasanovich, Siddiqov Abdujalol Siddiqovich, and Ikromov Abduvahob Ikromovich. "Obtaining Ethyl Acetate Based On Ether-Aldehyde Fraction And Acetic Acid." American Journal of Interdisciplinary Innovations and Research 02, no. 11 (2020): 47–53. http://dx.doi.org/10.37547/tajiir/volume02issue11-09.

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The article conducts systematic research on the processing of the ether-aldehyde fraction of the biochemical enterprises of the republic and the use of the purified ether-aldehyde fraction for the production of ethyl acetate. A detailed critical analysis of scientific, technical and patent literature in the field of production and use of esters and, in particular, ethyl acetate was carried out. Investigated the chemical composition of the ether-aldehyde fraction - the waste of the distilleries of the Republic. The optimal technological parameters for the purification of the ether-aldehyde frac

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16

Lau, Cher Haan, and Lee Suan Chua. "Solvation Free Energy Simulation for Rosmarinic Acid Extraction from Orthosiphon stamineus." Methods and Protocols 2, no. 3 (2019): 64. http://dx.doi.org/10.3390/mps2030064.

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This study was aimed to extract rosmarinic acid from Orthosiphon stamineus Benth. (Lamiaceae) in high yield. The mixture of chloroform–ethyl acetate (70:30) was chosen as the solvent system because rosmarinic acid gave the lowest solvation free energy in that solvent system based on the computational solubility prediction. The crude extract of the plant was fractionated by C18 reversed phase absorbent to recover rosmarinic acid. The content of rosmarinic acid was increased from 4.0% w/w to 6.7% w/w after fractionation. The radical scavenging activity of rosmarinic acid rich fraction (IC50 = 38

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Udie, Linus Ugbong, Effanga Offiong Offiong, Marvelous Christopher Etuk, and Ayodele Ebenezer Anjorin. "The Impact of Heavy Metals on the Soil in Calabar Metropolis Lemna Area." International Journal of Innovative Science and Research Technology 8, no. 1 (2023): 248–53. https://doi.org/10.5281/zenodo.7557972.

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Soil samples were collected from the dumpsite soil located at Lemna Road, Calabar Municipality, Cross River State, Nigeria to investigate the speciation and bioavailability of some trace metals namely: iron, cadmium, copper, zinc and lead. Extract from the soil were obtained by sequential extraction. This involved Acid extractable fraction, Oxidisable fraction, Reducible fraction and Residual fraction. These fraction were then analyzed for Pb, Cd, Cu, Fe and Zn using acetylene flame atomic absorption spectrophometer. The results obtained, showed that the metals occurred with the following perc

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Salih, Enass Y. A., Riitta Julkunen-Tiitto, Olavi Luukkanen, Marketta Sipi, Mustafa K. M. Fahmi, and Pia Johanna Fyhrquist. "Potential Anti-Tuberculosis Activity of the Extracts and Their Active Components of Anogeissus leiocarpa (DC.) Guill. and Perr. with Special Emphasis on Polyphenols." Antibiotics 9, no. 7 (2020): 364. http://dx.doi.org/10.3390/antibiotics9070364.

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In Sudanese traditional medicine, decoctions of the stem bark of Anogeissus leiocarpa are used for the treatment of tuberculosis (TB). However, this plant has not been investigated before for its antimycobacterial effects. Our screening results show, for the first time, that many extracts of various parts of A. leiocarpa exhibit growth inhibitory activity against Mycobacterium smegmatis. Minimum inhibitory concentration (MIC) values ranged between 625 and 5000 µg/mL, with an ethyl acetate extract of the root showing the lowest MIC value. The good antimycobacterial effects of the root part coul

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Alim, MA, JH Lee, CR Lee, CC Akoh, and KT Lee. "Characterization of Solvent-Fractionated Mustard Oil by High Performance Liquid Chromatography." Progressive Agriculture 19, no. 1 (2013): 135–41. http://dx.doi.org/10.3329/pa.v19i1.17364.

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Mustard oil (MO) was fractionated at low temperature with acetone. The liquid fraction obtained at -24°C, showed higher levels of oleic (24.7%) and linoleic (22.0%) acids, and lower level of erucic acid (24.5%) compared to other fractions. The lowest temperature (-24°C) was most effective for lowering the level of erucic acid through crystallization of triacylglycerol (TAG) molecules. One purpose of this study was to obtain erucic acid reduced fractions because erucic acid has undesirable effect on human health. The result suggested that erucic acid could be separates as solid fraction thereby

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Calderon-Chiu, Carolina, Montserrat Calderón-Santoyo, and Juan Arturo Ragazzo-Sánchez. "Protein Fractions of Jackfruit Leaf Flour and Protein Concentrate: Amino Acid Profile, Functional Properties and Thermal Analysis." Applied Sciences 14, no. 20 (2024): 9155. http://dx.doi.org/10.3390/app14209155.

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This study aims to obtain protein fractions, such as albumin, globulin, prolamin, and glutelin, from jackfruit leaf flour and protein concentrate (LPC). The protein fractions were characterised based on their protein content, amino acid profile, hydrosolubility, emulsifying, foaming, and thermal properties. The flour and LPC are mainly composed of prolamin and glutelin, respectively. The glutelin fractions presented a higher protein content and amino acid profile, featuring elevated leucine, valine, and glutamic acid levels. The glutelin fraction of LPC exhibited the highest hydrosolubility (2

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Rohadi, Rohadi, Umar Santoso, Sri Raharjo, and Iip Izul Falah. "Determination of Antioxidant Activity and Phenolic Compounds of Methanolic Extract of Java Plum (Syzygium cumini Linn. (Skeel) Seed." Indonesian Food and Nutrition Progress 14, no. 1 (2017): 9. http://dx.doi.org/10.22146/ifnp.24279.

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Methanolic extract of Java Plum (Syzygium cumini L. (Skeel) seed (MEJS) is potential source of natural antioxidant. As indicated by several in vitro measurements, the extract had strong DPPH (1,1 diphenyl, 2–picryl hydrazyl) and ABTS (2,2-azinobis, 3-ethylbenzothiazoline-6-sulphonate) radical scavenging activity, strong Ferric Reducing Antioxidant Power (FRAP) and moderate inhibition activity of linoleic acid oxidation. This study aimed to determine antioxidant activity and phenolic compound of Java Plum seed (Syzygium cumini L. (Skeel) methanolic extract fractions. Phenolics compound identifi

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Baker, R. Roy, and H. Y. Chang. "The acylation of 1-acyl-sn-glycero-3-phosphate by neuronal nuclei and microsomal fractions of immature rabbit cerebral cortex." Biochemistry and Cell Biology 68, no. 3 (1990): 641–47. http://dx.doi.org/10.1139/o90-091.

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The acylation of 1-acyl-sn-glycero-3-phosphate to form phosphatidic acid was studied using a neuronal nuclear fraction N1 and microsomal fractions P3, R (rough), S (smooth), and P (neuronal microsomes from nerve cell bodies) isolated from cerebral cortices of 15-day-old rabbits. The assays contained this lysophospholipid, ATP, CoA, MgCl2, NaF, dithiothreitol, and radioactive palmitate, oleate, or arachidonate. Of the subfractions, N1 and R had the highest specific activities (expressed per micromole phospholipid in the fraction). The rates with oleate were two to four times the values seen for

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Felipe González, Alejandro, Ivones Hernández Balmaseda, Yamilet I. Gutiérrez Gaitén, et al. "Phytochemical study and antioxidant capacity of three fractions from the stem of Caesalpinia bahamensis Lam." Journal of Pharmacy & Pharmacognosy Research 7, no. 1 (2019): 12–20. http://dx.doi.org/10.56499/jppres18.421_7.1.12.

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Context: Caesalpinia bahamensis Lam. is a medicinal plant used by the Cuban population to treat renal and hepatic diseases. However, this species lacks scientific studies that support its biological applications. Aims: To evaluate the chemical composition and the antioxidant capacity of fractions obtained from the stem of Caesalpinia bahamensis Lam. Methods: A continuous extraction of the stem was made by maceration using a battery of solvents of increasing polarity: chloroform, ethyl acetate and methanol. All fractions were analyzed by TLC and phytochemical screening. The compounds of the chl

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LOU, ZAIXIANG, CHENG LI, XINGRAN KOU, et al. "Antibacterial, Antibiofilm Effect of Burdock (Arctium lappa L.) Leaf Fraction and Its Efficiency in Meat Preservation." Journal of Food Protection 79, no. 8 (2016): 1404–9. http://dx.doi.org/10.4315/0362-028x.jfp-15-576.

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ABSTRACT First, the antibacterial, antibiofilm effect and chemical composition of burdock (Arctium lappa L.) leaf fractions were studied. Then, the efficiency of burdock leaf fractions in pork preservation was evaluated. The results showed that burdock leaf fraction significantly inhibited the growth and biofilm development of Escherichia coli and Salmonella Typhimurium. MICs of burdock leaf fractions on E. coli and Salmonella Typhimurium were both 2 mg/ml. At a concentration of 2.0 mg/ml, the inhibition rates of the fraction on growth and development of E. coli and Salmonella Typhimurium biof

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Tikhonov, Sergey L., and Natalya V. Tikhonova. "Study of protein fractions of secondary food raw materials." Far Eastern Agrarian Herald 18, no. 4 (2024): 113–19. https://doi.org/10.22450/1999-6837-2024-18-4-113-119.

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Peptides isolated from fish protein hydrolysates can be used in food systems as functional ingredients of specialized food products. A study was conducted to determine the fractional composition and amino acid sequence of the protein of the pink salmon innards. The crushed innards of chilled pink salmon were used as an object of research. Extraction methods based on the use of ammonium sulfate were used to isolate proteins. The amino acid sequence in a peptide was determined by mass spectrometric method. Two protein fractions were isolated in the chilled pink salmon innards. The amino acid seq

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Haryadi, Winarto, and Sugeng Triono. "FRACTIONATION OF FATTY ACID OMEGA 3, 6 AND 9 FROM SNAIL (Achatina fulica) USING COLOUM CHROMATOGRAPHY." Indonesian Journal of Chemistry 6, no. 3 (2010): 316–21. http://dx.doi.org/10.22146/ijc.21739.

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The extraction of fat from snail has been carried out by Soxhlet extractor with petroleum ether solvent. Fatty product from extraction was transesterificated in BF3/methanol solvent for an hour by reflux procedure, then extracted by n-hexane to produce methyl ester fatty acid. Free water fatty acid methyl ester was analyzed by Gas Chromatography-Mass spectrometer (GC-MS). Fatty acid ester was separated from its fractions use column chromatography in n-hexane, n-hexane:dietil eter (2:1 v/v), dietil eter, aseton, ethanol and methanol. This fractions wer also analized by GC-MS. From GC-MS data sh

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Liu, Borui, Qing Huang, Yuefeng Su, Mengyuan Wang, Ryan M. Kelly, and Liuye Sun. "Speciation of nickel and enzyme activities in fluvo-aquic soil under organic amendments treatment." Soil Research 56, no. 5 (2018): 456. http://dx.doi.org/10.1071/sr17330.

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To investigate the efficacy of humic acid and cow dung for nickel (Ni) immobilisation and detoxification in fluvo-aquic soil, the Ni-polluted soil was mixed with amendments of humic acid and cow dung. The Ni speciation (modified BCR sequential extraction method) and enzyme activities were scrutinised. As the humic acid concentration increased (0 to 12 g kg–1), the amount of Ni in the acetic acid-soluble fraction (the bioavailable fraction with high environmental risk) first decreased and then increased. In contrast, the amount of Ni in this fraction continued to decrease as the cow dung concen

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Yeh, Chun-Lang. "Numerical study of the burner parameters on the thermal field in a sulfur recovery unit thermal reactor." MATEC Web of Conferences 169 (2018): 01013. http://dx.doi.org/10.1051/matecconf/201816901013.

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A sulfur recovery unit (SRU) thermal reactor is the most important equipment in a sulfur plant and is negatively affected by high temperature operations. In this paper, the effect of burner parameters, including the clearance of the acid gas tip and the inlet air swirler angle, on the thermal field in a SRU thermal reactor are investigated numerically, with the aim to reduce the high temperature inside the thermal reactor and to ensure an acceptable sulfur recovery. The simulation results show that the burner with a smaller clearance of the acid gas tip produces a lower temperature, a lower ex

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Smith, C. E., J. Paiement, and J. J. Bergeron. "Subcellular distribution of acid NADPase activity within the parenchymal cells of rat liver." Journal of Histochemistry & Cytochemistry 34, no. 5 (1986): 649–58. http://dx.doi.org/10.1177/34.5.3009603.

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We examined the distribution of acid NADPase activity in rat hepatocytes by cytochemistry and subcellular fractionation. Cytochemical studies revealed strong NADPase activity in many lysosomes and Golgi saccules situated between the medial and trans aspect of the stack. Light, spotty deposits of reaction product also were observed frequently within elements of the Golgi-associated endoplasmic reticulum lysosome (GERL) system. Biochemical studies with liver homogenates indicated that NADPase activity was distributed across various subcellular fractions in a pattern resembling typical nonspecifi

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Darmawan, Akhmad, Maulidiyah Maulidiyah, Megawati Megawati, et al. "Cytotoxic of Usnic Acid Isolated from Ramalina sp." Journal of Tropical Life Science 14, no. 02 (2024): 349–56. http://dx.doi.org/10.11594/jtls.14.02.14.

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Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purifi

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Silva, Taina Kleinubing da, Ester Hadassa Neves, Andrey Felipe Kummer, et al. "Pfaffia glomerata: Gallic acid and phenolic compounds determination." Research, Society and Development 11, no. 17 (2022): e37111738706. http://dx.doi.org/10.33448/rsd-v11i17.38706.

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Pfaffia glomerata, known as ginseng-brasileiro, is a root widely used in folk medicine, being effective against inflammation, rheumatism, and fatigue, in addition to present aphrodisiacs and antidiabetics properties. Among the classes of substances reported for the specie, the phenolic acids, in special gallic acid, has shown positive activity to learning and memory, having a considerable effect against neurotoxic and pro-oxidant substances. Therefore, the article’s objective was to quantify gallic acid in the chloroform and ethyl acetate fractions from P. glomerata. Thus, the aerial parts of

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Manilal, Aseer, Sugathan Sujith, Balu Sabarathnam, et al. "Biological activity of the red alga Laurencia brandenii." Acta Botanica Croatica 70, no. 1 (2011): 81–90. http://dx.doi.org/10.2478/v10184-010-0001-x.

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Biological activity of the red algaLaurencia brandeniiThe marine red algaLaurencia brandeniicollected from the southwest coast of India (Indian Ocean) was extracted and fractioned using column chromatography. The individual fractions were evaluatedin vitrovia antimicrobial activity against six species of Microbial Type Culture Collection and three species of clinical human pathogens, antipest activity onSitophilus oryzae, maggoticidal activity against 2ndinstar larvae ofSarcophagasp. and termiticidal activity againstMicrotermes obesi.It was found that the fraction eluted using petroleum ether:

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Knepp, T. N., T. L. Renkens, and P. B. Shepson. "Gas phase acetic acid and its qualitative effects on snow crystal morphology and the quasi-liquid layer." Atmospheric Chemistry and Physics 9, no. 20 (2009): 7679–90. http://dx.doi.org/10.5194/acp-9-7679-2009.

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Abstract. A chamber was constructed within which snow crystals were grown on a string at various temperatures, relative humidities, and acetic acid gas phase mole fractions. The temperature, relative humidity, and acid mole fraction were measured for the first time at the point of crystal growth. Snow crystal morphological transition temperature shifts were recorded as a function of acid mole fraction, and interpreted according to the calculated acid concentration in the crystal's quasi-liquid layer, which is believed to have increased in thickness as a function of acid mole fraction, thereby

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Sławińska, Natalia, Magdalena Kluska, Barbara Moniuszko-Szajwaj, Anna Stochmal, Katarzyna Woźniak, and Beata Olas. "New Aspect of Composition and Biological Properties of Glechoma hederacea L. Herb: Detailed Phytochemical Analysis and Evaluation of Antioxidant, Anticoagulant Activity and Toxicity in Selected Human Cells and Plasma In Vitro." Nutrients 15, no. 7 (2023): 1671. http://dx.doi.org/10.3390/nu15071671.

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It is known that phenolic compounds can alleviate the negative impact of oxidative stress and modulate hemostasis. However, the effect of extracts and phenolics from Glechoma hederacea L. on the biomarkers of these processes is not well documented. The aim of our study was to investigate the in vitro protective effects of one extract and three fractions (20, 60, and 85% fraction) from G. hederacea L. on oxidative stress and hemostasis. Phytochemical analysis showed that aerial parts of G. hederacea L. are rich in both phenolic acids (such as rosmarinic acid, neochlorogenic acid, and chlorogeni

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Xiong, Shuang-li, Zheng-yu Jin, and An-lin Li. "Bioactive Composition of Pig Laryngeal Cartilage Extracts and Their Free Radical-scavenging Activity." Food Science and Technology International 12, no. 5 (2006): 371–77. http://dx.doi.org/10.1177/1082013206070410.

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This work focused on amino acid and mineral element analysis of pig laryngeal cartilage. Papain and DEAE-Sepharose fast flow ion exchange chromatography were used to hydrolyase cartilage and purify hydrolysate. Further study was performed to compare the free radical-scavenging activity of various fractions. Cartilage is abundant in bioactive amino acids such as glycine, glutamic acid and arginine and minerals such as calcium, magnesium and iron. Four polysaccahrides attached peptides and one peptide were isolated from hydrolysate. Weak 2,2-diphenyl-1-picryhydrazyl free radical scavenging activ

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Sumi Lestari, Fajar, Ricson Pemimpin Hutagaol, and Srikandi Srikandi. "Crystal guava (Psidium guajava L. “Crystal”) treated with gibberelic acid (GA3) : Evaluation of in vitro antioxidant capacities." Jurnal Pendidikan Kimia 15, no. 2 (2023): 82–90. http://dx.doi.org/10.24114/jpkim.v15i2.41780.

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Free radicals can cause many diseases, such as cancer. Antioxidant is a compound that could scavenge free radicals. One of the natural source antioxidants is guava. The application of gibberellic acid (GA3) is known to affect the physicochemical properties and antioxidant content of the fruit. The goals of this research were to investigate the antioxidant activity of skin, flesh and fruit of crystal guava were given additional GA3 treatment with concentration 0; 60; 120; 180; 240; and 300 ppm by determining the value of the Antioxidant Activity Index (AAI) using DPPH; evaluate the total phenol

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Ramasamy, P., and S. Lekha Panicker. "Studies on the chemical nature of the cyst wall of Microphallus madrasensis." Journal of Helminthology 65, no. 2 (1991): 111–19. http://dx.doi.org/10.1017/s0022149x00010555.

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ABSTRACTThe cyst wall of Microphallus madrasensis resisted 6 N and 11·3 N hydrochloric acids (HCL) 36 N concentrated sulphuric acid (H2S04) and 0·35 M sodium hypochlorite. It was partially dissolved in 0·6 M sodium azide and completely dissolved in 16 N nitric acid (HNO3). An HCl hydrolysate of the cyst wall contained 10 amino acids viz. alanine, leucine, aspartic acid, glutamic acid, lysine, histidine. phenylalanine. tyrosine. cystine and proline. Polyacrylamide gel electrophoresis revealed the presence of a fast moving fraction which stained with periodic acid-Schiffs (PAS) reagent and tolui

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Sugita, Purwantiningsih, Dina Anggraini, Gustini Syahbirin, Dyah Utami Cahayaning Rahayu, and Auliya Ilmiawati. "Secondary Metabolites from Indonesian Kigelia africana (Bignoniaceae)." Journal of the Indonesian Chemical Society 2, no. 2 (2019): 114. http://dx.doi.org/10.34311/jics.2019.02.2.114.

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From the fruit of Kigelia africana three fractions of secondary metabolites were isolated. Fraction 1 (CD-1) was characterized by using UV-Vis, FTIR, 1D and 2D NMR and MS, meanwhile fraction 2 (CD-2) and 3 (B-1) were characterized by LCMS and compared with literature. All these fractions were screened for anticancer activity by using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method to calculate IC50 toward Michigan Cancer Foundation-7 MCF-7 breast cancer and menogaril-resistant mouse leukemia P388 cells. Based on spectroscopic data, CD-1 fraction was identified as me

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Zhang, Li Hua, and Cheng Song Zheng. "Copper Distribution in Shaxi River Sediments." Advanced Materials Research 356-360 (October 2011): 1440–44. http://dx.doi.org/10.4028/www.scientific.net/amr.356-360.1440.

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For the surficial sediments sampled from the Shaxi River in Sanming, Fujian Province, China, the distribution characteristic of heavy metal copper in surficial sediments were investigated. The microwave digestion result showed that the concentration of Cu in surficial sediments ranged from 26.95 mg/kg to 74.76 mg/kg. The results of modified BCR sequential extraction procedure showed that heavy metal copper in the surficial sediment was mainly associated with residual and reducible fractions. The fractions of copper sampled near the wastewater discharge were in the order of residual > reduci

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Pambayun, Lintang Panjali Siwi, Benito Heru Purwanto, and Sri Nuryani Hidayah Utami. "Carbon Stock, Carbon Fraction and Nitrogen Fraction of Soil Under Bamboo (Dendrocalamus asper Back.) and Non-Bamboo Vegetation." Caraka Tani: Journal of Sustainable Agriculture 38, no. 2 (2023): 404. http://dx.doi.org/10.20961/carakatani.v38i2.75881.

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<span lang="EN-US">The type of vegetation and soil organic matter affect the carbon fraction, nitrogen fraction and soil carbon stocks that contribute to the global carbon cycle. Therefore, the calculation of the composition of the fractions in different land covers is very important as a potential indicator of the effect of land management practices on soil organic carbon dynamics and supports the reduction of carbon dioxide (CO<sub>2</sub>) and soil carbon storage. This research aimed to determine the composition of the carbon fraction, nitrogen fraction and soil carbon sto

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Karimi, Ali, Mohammad-Taghi Moradi, Mohammad Rabiei, and Somayeh Alidadi. "In vitro anti-adenoviral activities of ethanol extract, fractions, and main phenolic compounds of pomegranate (Punica granatum L.) peel." Antiviral Chemistry and Chemotherapy 28 (January 2020): 204020662091657. http://dx.doi.org/10.1177/2040206620916571.

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Background Adenovirus causes a number of diseases in human, and can cause serious infection in severely immunosuppressed individuals. Despite the seriousness of adenovirus infection, there is no definitely approved anti-adenoviral therapy. Many studies have shown that compounds derived from medicinal plants have antiviral activity. Therefore, this study evaluated in vitro anti-adenoviral activity of ethanol extract, fractions, and main phenolic compounds of pomegranate peel. Methods The ethanol extract of pomegranate peel was prepared with maceration method and fractionated by consecutive liqu

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Gazali, Mohamad, Odi Jolanda, Amir Husni та ін. "In Vitro α-Amylase and α-Glucosidase Inhibitory Activity of Green Seaweed Halimeda tuna Extract from the Coast of Lhok Bubon, Aceh". Plants 12, № 2 (2023): 393. http://dx.doi.org/10.3390/plants12020393.

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Seaweed belongs to marine biota and contains nutrients and secondary metabolites beneficial for health. This study aimed to determine the antidiabetic activity of extracts and fractions of green seaweed Halimeda tuna. The H. tuna sample was extracted with the maceration method using methanol and then partitioned using ethyl acetate and water to obtain ethyl acetate and water fractions. The methanol extract, ethyl acetate fraction, and water fraction of H. tuna were tested for their inhibitory activity against α-amilase and α-glucosidase. The methanol extract and the fractions with the highest

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Permatasari, Ni Made Ayu Dinda, Handa Muliasari, Nisa Isneni Hanifa, et al. "Anti-Radical Activity Test of Fractions from Ashitaba Herbs (Angelica keiskei)." Jurnal Biologi Tropis 24, no. 4 (2024): 492–500. https://doi.org/10.29303/jbt.v24i4.7638.

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Acne vulgaris is a chronic inflammation of the skin’s pilosebaceous unit, caused by oxidative stress due to reactive oxygen species (ROS) release. Oxidative stress conditions can be treated using natural antioxidants, namely ashitaba (Angelica keiskei). In contrast to previous studies that tested column chromatography fraction of the macerated ethanol extract, this study examined the liquid-liquid partition fraction of 80% methanol sonicated extract of Ashitaba herb. This study aims to determine the antiradical activity of various fractions of Ashitaba herb extract against 1,1-diphenyl-2-picry

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Dalli, Mohammed, Nour Elhouda Daoudi, Salah-eddine Azizi, Hind Benouda, Mohamed Bnouham та Nadia Gseyra. "Chemical Composition Analysis Using HPLC-UV/GC-MS and Inhibitory Activity of Different Nigella sativa Fractions on Pancreatic α-Amylase and Intestinal Glucose Absorption". BioMed Research International 2021 (26 червня 2021): 1–13. http://dx.doi.org/10.1155/2021/9979419.

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Nigella sativa (NS) is a well-known plant for its various benefits and multiuse in traditional medicine. This study is aimed at investigating the chemical composition of the different NS fractions by using GC-MS for the esterified fatty acids or HPLC-UV for organic fraction and at evaluating the inhibitory effect on pancreatic α-amylase (in vitro, in vivo) and intestinal glucose absorption. Among all the investigated fractions, it was shown that they are rich with different molecules of great interest. The n-hexane fraction was characterized by the presence of linoleic acid (44.65%), palmitic

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Zhouyong, Dong, Tian Gang, Xu Zhaogang, Li Mingyue, Xu Min, and Ren Yajun Zhou and Hui. "Antioxidant activities of peptide fractions derived from freshwater mussel protein using ultrasound-assisted enzymatic hydrolysis." Czech Journal of Food Sciences 35, No. 4 (2017): 328–38. http://dx.doi.org/10.17221/421/2016-cjfs.

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The freshwater mussel protein was hydrolysed using ultrasound-assisted enzymolysis. Ultrasound-assisted freshwater mussel protein hydrolysates (UPH) were divided into four fractions (&gt; 10, 6–10, 3–6, and &lt; 3 kDa) using ultrafiltration, and the fraction with the highest antioxidant activity was further subdivided into four fractions (F<sub>1</sub>–F<sub>4</sub>) using gel chromatography. The amino acid compositions and antioxidant activities (DPPH, hydroxyl and superoxide radical scavenging activities, reducing power, ferrous ion chelating activity, and inh

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Kim, Yi Kyeoung, Jae-Min Lim, Young Jae Kim, and Wook Kim. "Alterations in pH of Coffee Bean Extract and Properties of Chlorogenic Acid Based on the Roasting Degree." Foods 13, no. 11 (2024): 1757. http://dx.doi.org/10.3390/foods13111757.

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Factors influencing the sour taste of coffee and the properties of chlorogenic acid are not yet fully understood. This study aimed to evaluate the impact of roasting degree on pH-associated changes in coffee bean extract and the thermal stability of chlorogenic acid. Coffee bean extract pH decreased up to a chromaticity value of 75 but increased with higher chromaticity values. Ultraviolet–visible spectrophotometry and structural analysis attributed this effect to chlorogenic and caffeic acids. Moreover, liquid chromatography-mass spectrometry analysis identified four chlorogenic acid types in

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Suleiman, Mikailu, and Joy Nkemkanma Iwedi. "Phytochemical and Cytotoxic Evaluation of the Fruits of Rauwolfia vomitoria Afzel. (Apocynaceae)." Journal of Cancer and Tumor International 15, no. 1 (2025): 60–66. https://doi.org/10.9734/jcti/2025/v15i1282.

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Rauwolfia vomitoria fruits has been claimed to be used traditionally in treating cancer related diseases. However, this activity has not yet been scientifically proven. This study focused on investigating the cytotoxicity activity of this plant on Tadpoles (Raniceps raninus). The powdered fruits was extracted with methanol and partitioned with n-hexane, dichloromethane and ethyl acetate. The extract and fractions obtained were evaluated for cytotoxic potential in tadpoles. Bioactive fractions were analyzed on Gas chromatography-Mass spectrometry. The n-hexane fraction showed significant cytoto

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Shoeb, Mohammad, Md Nazrul Islam, and Nilufar Nahar. "Biological activity studies of the aerial parts of Phyllanthus niruri L." Current Research on Biosciences and Biotechnology 4, no. 1 (2022): 251–55. http://dx.doi.org/10.5614/crbb.2022.4.1/u7gmg56e.

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Phyllanthus niruri L. is a small annual plant grows up to 30-40 cm tall. It is an herbaceous plant that is widely distributed in the tropical and sub-tropical regions. The aerial parts of P. niruri growing in Bangladesh were investigated for moisture content and ash content, total phenolic content, total flavonoid content, total antioxidant capacity (TAC), cytotoxicity, relative fatty acid compositions and antibacterial activity. The moisture and ash content of the dried leaves were 5 and 7%, respectively. The extracts of P. niruri contained a good amount of phenolic and flavonoid content and

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Indariani, Susi, Aisyah Hidayat, Latifah K. Darusman, and Irmanida Batubara. "ANTIBACTERIAL ACTIVITY OF FLAVONOID FROM KEPEL (STELECHOCARPUS BURAHOL) LEAVES AGAINST STAPHYLOCOCCUS EPIDERMIDIS." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 10 (2017): 292. http://dx.doi.org/10.22159/ijpps.2017v9i10.19071.

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Objective: The objective of this research was to separate flavonoids of kepel (Stelechocarpus burahol) leaves from methanol extract as antibacterial agent against Staphylococcus epidermidis that have a role in body odor.Methods: The methanol extracts of kepel was fractionated in methanol: water (7:3), n-hexane and chloroform consecutively. Methanol: water (7:3) extract with the highest flavonoids content, fractioned by silica gel column chromatography (isocratic elution, with n-buthanol: methanol: acetic acid (1:8:1) as eluent) to produce 7 fractions. All fractions were tested for antibacteria

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Hasan, Hayder T., and Enas J Kadhim. "PHYTOCHEMICAL INVESTIGATION OF LEAVES AND SEEDS OF CORCHORUS OLITORIUS L. CULTIVATED IN IRAQ." Asian Journal of Pharmaceutical and Clinical Research 11, no. 11 (2018): 408. http://dx.doi.org/10.22159/ajpcr.2018.v11i11.28408.

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Objective: The aim of this study was to investigate chemical constituents of leaves and seeds of Corchorus olitorius since no phytochemical investigation had been done previously in Iraq.Methods: Leaves and seeds of C. olitorius were defatted by maceration in hexane for 24 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous methanol 85% as a solvent extraction for 24 h, and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The ethyl acetate, n-butanol, and n-butanol after hydrolysis fractions for each part (leaves and seeds) were analyze

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