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1

Sharma, S. (Satyan). "Computational Studies on Prostatic Acid Phosphatase." Doctoral thesis, University of Oulu, 2008. http://urn.fi/urn:isbn:9789514289743.

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Abstract Histidine acid phosphatases are characterized by the presence of a conserved RHGXRXP motif. One medically important acid phosphatase is the Prostatic Acid Phosphatase (PAP). PAP has been associated with prostate cancer for a long time and has been used as a marker to stage prostate carcinoma. Yet, there is no clear understanding on the functioning of the enzyme in vivo. This thesis work focuses on the characterization of putative ligands and elucidation of the reaction mechanism of PAP using computational methods. The ligand-enzyme complexes were generated by docking and molecular d
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2

Gellatly, Kevin Scott. "Biochemical and molecular characterization of potato tuber acid phosphatase and other members of the plant acid phosphatase family." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ31926.pdf.

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3

Kolba, Clifford Andrew. "Intracellular and extracellular acid phosphatase activity in axenically cultured phosphate-deprived achlorophyllous euglena gracilis /." Access Digital Full Text version, 1994. http://pocketknowledge.tc.columbia.edu/home.php/bybib/11714372.

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Thesis (Ed.D.)--Teachers College, Columbia University, 1994.<br>Typescript; issued also on microfilm. Sponsor: O. Roger Anderson. Dissertation Committee: Patricia L. Dudley. Includes tables. Includes bibliographical references (leaves 119-129).
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4

Hunter, Roland. "The secretion of acid phosphatase by proteaceae as an adaptation to limited phosphate availability." Master's thesis, University of Cape Town, 2010. http://hdl.handle.net/11427/12173.

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Includes abstract.<br>Includes bibliographical references (leaves 59-67).<br>The Proteaceae are a distinctive component of South Africa’s Fynbos and Australia’s Kwongan vegetation and display a number of adaptations to the soils of these nutrientpoor regions, where availability of phosphate (P) is particularly limited. The Proteaceae are notable for the virtually family-wide ability to form cluster roots.
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5

Ljusberg-Sjölander, Jenny. "Regulation of tartrate-resistant purple acid phosphatase by proteolytic processing in rat /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-208-X/.

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6

Johansson, Eva. "Structural studies of saccharopine reductase and purple acid phosphatase /." Stockholm, 2000. http://diss.kib.ki.se/2000/91-628-4284-6/.

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7

Nakagi, Vanessa de Souza. "Caracterização da atividade da fosfatase ácida de Penicillium implicatum /." Jaboticabal : [s.n.], 2007. http://hdl.handle.net/11449/94888.

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Orientador: João Martins Pizauro Júnior<br>Banca: Maria Inês Tiraboschi Ferro<br>Banca: Pietro Ciancaglini<br>Resumo: A produção de fosfatase ácida extracelular pelo fungo Penicillium implicatum foi estudada em diferentes concentrações de fosfato no meio de cultivo e na presença e ausência de agitação. A produção de fosfatase ácida extracelular repressível foi cerca de 15 vezes maior em meio agitado e na presença de 50 ìM de KH2PO4 quando comparada ao tratamento controle. A enzima foi purificada 19 vezes em Fenil Sepharose CL-4B, e apresenta uma atividade específica de 27,3U/mg. A enzima é um
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8

Rombola, Tiago Henrique [UNESP]. "Indução da expressão e caracterização de uma fosfatase ácida ligada à membrana produzida por Burkholderia sp." Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/94890.

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Made available in DSpace on 2014-06-11T19:27:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-08-11Bitstream added on 2014-06-13T20:56:20Z : No. of bitstreams: 1 rombola_th_me_jabo.pdf: 290913 bytes, checksum: 08f244b9cd35a229582ac7563c4b4ff9 (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>Espécies de Burkholderia filogenéticamente distantes do complexo B. cepacia, são versáteis solubilizadores de minerais insolúveis, através da produção de ácidos, viabilizando nutrientes para as plantas. Descrevemos a purificação e caracterização da fosfatase ácid
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9

Yeung, Sin-lui, and 楊倩蕾. "A study of purple acid phosphatase from Burkholderia cenocepacia." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B41508373.

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10

Yeung, Sin-lui. "A study of purple acid phosphatase from Burkholderia cenocepacia." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B41508373.

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11

Orkwis, Brianne. "Novel acid phosphatase in Candida glabrata suggests dynamic selective pressures on the phosphate starvation response." Click here for download, 2009. http://proquest.umi.com.ps2.villanova.edu/pqdweb?did=1934097791&sid=1&Fmt=2&clientId=3260&RQT=309&VName=PQD.

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12

Ek-Rylander, Barbro. "Studies on the structure and function of the purple acid phosphatase of rat osteoclasts." Stockholm : Dept. of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, 1995. http://catalog.hathitrust.org/api/volumes/oclc/33858972.html.

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13

Felts, Richard Levi. "Structural studies of acid phosphatases from pathogenic bacteria." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4857.

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Thesis (Ph.D.)--University of Missouri-Columbia, 2007.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on March 23, 2009) Vita. Includes bibliographical references.
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14

Nakagi, Vanessa de Souza [UNESP]. "Caracterização da atividade da fosfatase ácida de Penicillium implicatum." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/94888.

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Made available in DSpace on 2014-06-11T19:27:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-03-27Bitstream added on 2014-06-13T19:55:55Z : No. of bitstreams: 1 nakagi_vs_me_jabo.pdf: 402591 bytes, checksum: bde6a86608bbb9a9d68eb164ee5cc8ca (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>A produção de fosfatase ácida extracelular pelo fungo Penicillium implicatum foi estudada em diferentes concentrações de fosfato no meio de cultivo e na presença e ausência de agitação. A produção de fosfatase ácida extracelular repressível foi cerca de 15 vezes mai
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15

Rombola, Tiago Henrique. "Indução da expressão e caracterização de uma fosfatase ácida ligada à membrana produzida por Burkholderia sp. /." Jaboticabal : [s.n.], 2008. http://hdl.handle.net/11449/94890.

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Orientador: João Martins Pizauro Junior<br>Banca: Maria Inês Tiraboschi Ferro<br>Banca: Pietro Ciancaglini<br>Resumo: Espécies de Burkholderia filogenéticamente distantes do complexo B. cepacia, são versáteis solubilizadores de minerais insolúveis, através da produção de ácidos, viabilizando nutrientes para as plantas. Descrevemos a purificação e caracterização da fosfatase ácida ligada à membrana, produzida pela bactéria do gênero Burkholderia, isolada de solo agrícola em Ponta grossa-PR-BRASIL, e identificada através da análise do 16s rDNA. A expressão da enzima se mostrou estritamente depen
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16

Walsh, Nicole Cherie. "The regulation and function of murine tartrate resistant acid phosphatase /." St. Lucia, Qld, 2003. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe17572.pdf.

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17

Humphries, A. C. "The total synthesis of the protein phosphatase inhibitor okadaic acid." Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604785.

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This thesis is divided into seven chapters. The first is a brief discussion of the structure, regulation and rôle of the protein phosphatases in biological systems, with particular emphasis on the important contribution made by the protein phosphatase inhibitor okadaic acid. The second chapter is a review of published synthetic work towards okadaic acid by other research groups. Chapter three begins with a brief review of some synthetic strategies towards the spiroketal unit. The general approach adopted within these laboratories for the total synthesis of okadaic acid is then described, with
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18

Kuang, Ruibin, and 鄺瑞彬. "A study of a purple acid phosphatase in Arabidopsis thaliana." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B41896907.

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19

Sun, Feng, and 孙峰. "Functional role of a purple acid phosphatase in Arabidopsis thaliana." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46477019.

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20

Kuang, Ruibin. "A study of a purple acid phosphatase in Arabidopsis thaliana." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B41896907.

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21

Valizadeh, Mohsen. "Mechanistic and functional studies on purple acid phosphatases /." St. Lucia, Qld, 2003. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe17458.pdf.

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22

Chaves, Neto Antonio Hernandes [UNESP]. "Estudo das fosfatases ácidas e fosfatase alcalina na saliva e no soro de crianças." Universidade Estadual Paulista (UNESP), 2005. http://hdl.handle.net/11449/95460.

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Made available in DSpace on 2014-06-11T19:27:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-12-16Bitstream added on 2014-06-13T20:56:53Z : No. of bitstreams: 1 chavesneto_ah_me_araca.pdf: 216462 bytes, checksum: a077ffffb43a7f52121fc844bcf5ba50 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>A saliva é coletada através de métodos simples e não invasivos, além de ser facilmente armazenada. A coleta não-traumática é atrativa especialmente para crianças e quando repetidas coletas são requeridas. A desvantagem da saliva como uma ferramenta de diagnóstic
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23

Lång, Pernilla. "Tartrate resistant acid phosphatase in the immune and nervous system : distribution and pathophysiological implications /." Stockholm : Karolinska institutet, 2007. http://diss.kib.ki.se/2007/978-91-7357-402-0/.

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24

Balci, Ayse Tulay. "The use of milk enzymes as indices of high pressure treatment." Thesis, University of Reading, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365873.

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25

Sato, Vanessa Sayuri [UNESP]. "Indução da expressão in vivo e caracterização cinética da fosfatase ácida de Enterobacter sp. isolada de raízes de orquidáceas." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/94903.

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Made available in DSpace on 2014-06-11T19:27:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-03-29Bitstream added on 2014-06-13T19:35:22Z : No. of bitstreams: 1 sato_vs_me_jabo.pdf: 522350 bytes, checksum: 34c42a9a6f174fa4f2002e3d48b07c50 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>A capacidade de bactérias endofíticas em solubilizar fosfato inorgânico é alvo de grande interesse por parte dos microbiologistas, uma vez que as fosfatases são responsáveis por hidrolisar compostos orgânicos produzindo fósforo solúvel. Dessa forma, a fosfatase ácida
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26

Efstratiades, Theodore. "Human bone-type acid phosphatase : its distribution, properties and use in diagnosis." Thesis, Imperial College London, 1985. http://hdl.handle.net/10044/1/37682.

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27

Mennan, Claire. "Bacterial acid phosphatase and its application to waste remediation and metal recovery." Thesis, University of Birmingham, 2011. http://etheses.bham.ac.uk//id/eprint/1017/.

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PhoN-type acid phosphatase from an atypical Serratia sp. was applied to the removal of radionuclides from aqueous flows. In the presence of metal ions the phosphatase produces metal phosphate at the cell surface via the liberation of inorganic phosphate from a phosphate donor (glycerol-2-phosphate). Previous studies using biogenic hydrogen uranyl phosphate (HUP) on Serratia sp. removed \(^{60}\)Co, \(^{137}\)Cs and \(^�\)Sr via intercalative ion exchange into the HUP crystal lattice. Due to their non toxic and non radioactive nature, zirconium phosphates offer an alternative to HUP for the dec
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28

Monier, Antoine. "Rôle des phosphatases acides dans le métabolisme et le stockage des sucres dans la vacuole chez Arabidopsis thaliana : approches in vitro et in silico." Thesis, Bordeaux 1, 2012. http://www.theses.fr/2012BOR14709/document.

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Les « Purple Acid Phosphatases » (PAPs) sont des phosphatases acides présentes dans le règne végétal et animal. Elles sont caractérisées à la fois par la présence de sept acides aminés conservés impliqués dans la structure d’un centre bimétallique et par leur couleur rose/violète lorsqu’elles se trouvent en solution. Chez les plantes, certaines PAPs présenteraient à la fois une activité phosphatase sur des métabolites (PEP, G6P…) et sur des phospho-peptides (glycosidases…), d’une part, et une activité peroxydase, de l’autre. Ces PAPs pourraient donc, non seulement être impliquées dans le métab
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29

Conrad, Robyn. "The evaluation of the specificity of the Acid Phosphatase test to identify semen." Master's thesis, Faculty of Health Sciences, 2018. http://hdl.handle.net/11427/30096.

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The burden of sexual assault cases in South Africa is exceptionally high, and analysis of biological material in these cases may provide evidence towards a criminal investigation. These analyses include the identification of biological material to: (i) presumptively identify if semen may be present in order to guide downstream DNA profiling analysis to identify the alleged assailant; and (ii) confirm if semen was indeed present to provide evidence of ejaculation. The presumptive test used to detect the possible presence of semen is the Brentamine Fast Blue (FB) test, which detects the presence
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30

Ou, Zhonghui. "Structure of an antigen-binding fragment bound to stem-loop DNA and crystallization of recombinant haemophilus influenzae e(P4) acid phosphatase." Diss., Columbia, Mo. : University of Missouri-Columbia, 2006. http://hdl.handle.net/10355/4621.

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Thesis (M.S.)--University of Missouri-Columbia, 2006.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on April 17, 2009) Includes bibliographical references.
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31

Violet, Pierre-Christian. "Rôle des lipides-phosphate phosphatases dans la modulation des voies de signalisation impliquées dans les léiomyomes utérins." Phd thesis, Université Paris Sud - Paris XI, 2012. http://tel.archives-ouvertes.fr/tel-00788479.

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Le léiomyome utérin est la pathologie utérine la plus fréquente chez les femmes en âges de procréer. Des résultats précédent obtenus avec les cellules ELT3, une lignée de cellules de léiomyomes de rat, ont montré que l'acide lysophosphatidique (LPA) activait les MAP kinases ERK1/2 via le récepteur LPA1 couplé à la protéine Gi et l'activation de Raf, Ras et de MEK. Durant ce travail, nous avons caractérisé l'activité phosphatase responsable de la dégradation du LPA dans cette lignée de cellules ELT3. Nous avons montré que le LPA était dégradé exclusivement par la lipide-phosphate phosphatase 1
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32

Lamouroux, Lucille. "Essais de production de protéines phosphatases sensibles aux microcystines et à l'acide okadaïque pour la conception de biocapteurs." Toulouse 3, 2006. http://www.theses.fr/2006TOU30037.

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Les microcystines et l'acide okadaïque sont des toxines produites par les cyanobactéries et les dinoflagellés. Ces toxines posent un réel problème de santé publique. Elles sont fréquemment retrouvées à travers le monde, que ce soit les réservoirs d'eau, les lacs, les rivières, les mers. Leur ingestion provoque des troubles gastro-intestinaux et hépatiques pouvant conduire dans les cas les plus graves à la mort de l'individu. Leur détection est un enjeu majeur. Dans le cas des eaux de boisson, la norme de détection fixée par l'OMS est de 1µg/L. Une des méthodes permettant de détecter ces toxine
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33

Yu, Changhua. "Phospholipase D/phosphatidic acid phosphatase signal transduction pathway in post-infarction congestive heart failure." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq23565.pdf.

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34

Weber, Roland Wolfram Sixtus. "Biochemical, physiological and histochemical aspects of acid phosphatase secretion by Botrytis cinerea Pers.: Fr." Thesis, University of Exeter, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307294.

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35

Kreienbühl, Peter Lukas. "Protein phosphatase inhibitor okadaic acid alters cell shape and F-action distribution and inhibits /." Bern, 1992. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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36

Montero, Jimenez Maria Dorelis. "Neuroprotective effects of magnesium sulphate evaluated by fluoride resistant acid phosphatase, inorganic phosphate, lactate dehydrogenase and nitric oxide in rats with ischemia." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-423794.

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Brain ischemia events are among the first three mortalities causes in the world associated with high treatments costs and in most cases some degree of permanent functional disability, thus it is necessary with new low cost and effective treatments. In the present study the potential neuroprotective effect of magnesium sulphate was evaluated by quantification of ischemic biomarkers: fluoride resistant acid phosphatase (FRAP), inorganic phosphate (Pi), lactate dehydrogenase (LDH) and nitric oxide (NO) in rats with global ischemia. Adult rats were anaesthetized (sodium thiopental 60mg.kg-1) and m
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37

Cha, So Young. "Identification and characterisation of a secreted acid phosphatase, Aph1 in the fungal pathogen Cryptococcus neoformans." Thesis, The University of Sydney, 2015. http://hdl.handle.net/2123/13693.

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Human fungal pathogen Cryptococcus neoformans is a major cause of mortality among immunocompromised individuals. Extracellular acid phosphatase activity has been detected in >95% of the C. neoformans strains isolated from patients with AIDS, including the highly virulent serotype A strain H99. However the contribution of this activity to virulence of C. neoformans has never been determined. As part of this study, four putative acid phosphatases were identified in C. neoformans H99 genome: CNAG 02944 (Aph1), CNAG_06967 (Aph2), CNAG_02681 (Aph3) and CNAG_06115 (Aph4), of which only Aph1 is predi
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38

Patrikainen, L. (Lila). "Prostatic gene expression: probasin, human prostatic acid phosphatase and macrophage inhibitory cytokine-1 as model genes." Doctoral thesis, University of Oulu, 2004. http://urn.fi/urn:isbn:9514272730.

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Abstract Gene products that are only expressed in one tissue or cell type are useful models for investigating the biochemical and molecular mechanisms of tissue/cell-specific gene regulation. The regulatory regions of such genes are also practical tools in gene therapy. In this work, prostate-specific and androgen-dependent gene regulation was investigated by using human prostatic acid phosphatase (hPAP) and rat probasin (rPB) as models. In DNase I footprinting, a protected 12 bp region was found in the PB gene between the nucleotides -251 and -240 only with nuclear extracts of prostatic orig
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Issa, Amalia Mary. "Effects of the protein phosphatase inhibitors calyculin A and okadaic acid at the cholinergic nerve terminal." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0016/NQ44461.pdf.

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Patrikainen, Lila. "Prostatic gene expression : probasin, human prostatic acid phosphatase and macrophage inhibitory cytokine-1 as model genes /." Oulu : Oulun yliopisto, 2004. http://herkules.oulu.fi/isbn9514272730/.

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41

Dal, Prá Ketelin Juliane [UNESP]. "Efeitos da aguardente de cana em glândulas submandibulares de ratos: avaliação da atividade das fosfatases, níveis de mucina e histomorfometria." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/148573.

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Submitted by KETELIN JULIANE DAL PRÁ null (k.dalpra@hotmail.com) on 2017-01-20T02:22:12Z No. of bitstreams: 1 Dissertação de Mestrado Ketelin J. Dal Prá - FOA UNESP.pdf: 1380407 bytes, checksum: aa6eb3e50dc3c4e8a803bd7b396157fa (MD5)<br>Approved for entry into archive by LUIZA DE MENEZES ROMANETTO (luizamenezes@reitoria.unesp.br) on 2017-01-24T12:22:26Z (GMT) No. of bitstreams: 1 dalpra_kj_me_araca.pdf: 1380407 bytes, checksum: aa6eb3e50dc3c4e8a803bd7b396157fa (MD5)<br>Made available in DSpace on 2017-01-24T12:22:26Z (GMT). No. of bitstreams: 1 dalpra_kj_me_araca.pdf: 1380407 bytes, checks
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Chaves, Neto Antonio Hernandes. "Estudo das fosfatases ácidas e fosfatase alcalina na saliva e no soro de crianças /." Araçatuba : [s.n.], 2005. http://hdl.handle.net/11449/95460.

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Orientador: Ana Cláudia de Melo Stevanato Nakamune<br>Banca: José Mauro Granjeiro<br>Banca: Célio Percinoto<br>Resumo: A saliva é coletada através de métodos simples e não invasivos, além de ser facilmente armazenada. A coleta não-traumática é atrativa especialmente para crianças e quando repetidas coletas são requeridas. A desvantagem da saliva como uma ferramenta de diagnóstico é a variabilidade, que ocorre em decorrência dos fatores fisiológicos e do modo de coleta. Numa primeira etapa o trabalho teve por objetivo estudar as atividades das enzimas fosfatase alcalina (FAlc), fosfatase ácida
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43

Chou, Yu-Wei, Fen-Fen Lin, Sakthivel Muniyan, et al. "Cellular prostatic acid phosphatase (cPAcP) serves as a useful biomarker of histone deacetylase (HDAC) inhibitors in prostate cancer cell growth suppression." BioMed Central, 2015. http://hdl.handle.net/10150/610307.

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BACKGROUND: Prostate cancer (PCa) is the most commonly diagnosed solid tumor and the second leading cancer death in the United States, and also one of the major cancer-related deaths in Chinese. Androgen deprivation therapy (ADT) is the first line treatment for metastatic PCa. PCa ultimately relapses with subsequent ADT treatment failure and becomes castrate-resistant (CR). It is important to develop effective therapies with a surrogate marker towards CR PCa. METHOD: Histone deacetylase (HDAC) inhibitors were examined to determine their effects in androgen receptor (AR)/ cellular prostatic aci
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44

Stürzel, Christina [Verfasser]. "Fibril formation of naturally occurring prostatic acid phosphatase fragments and their effect on viral infection and transduction / Christina Stürzel." Ulm : Universität Ulm. Medizinische Fakultät, 2014. http://d-nb.info/1053706022/34.

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45

Sato, Vanessa Sayuri. "Indução da expressão in vivo e caracterização cinética da fosfatase ácida de Enterobacter sp. isolada de raízes de orquidáceas /." Jaboticabal : [s.n.], 2011. http://hdl.handle.net/11449/94903.

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Resumo: A capacidade de bactérias endofíticas em solubilizar fosfato inorgânico é alvo de grande interesse por parte dos microbiologistas, uma vez que as fosfatases são responsáveis por hidrolisar compostos orgânicos produzindo fósforo solúvel. Dessa forma, a fosfatase ácida ligada à membrana (MBAP) foi obtida a partir de Enterobacter sp. isolada de raízes de Cattleya walkeriana (Orchidaceae) e identificada pelo seqüenciamento do gene 16S rRNA. A expressão da enzima mostrou-se estritamente regulada pelo fósforo (expressão ideal em 7 mm). O pH ótimo aparente (3,5) não foi afetado pela concentra
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46

Bartonjo, Jane Jeruto. "Heterologous Expression of Arabidopsis Thaliana Purple Acid Phosphatase Gene (Atpap15) in Crops for Phytoremediation of Sites Contaminated with Excess Phosphorus." TopSCHOLAR®, 2015. http://digitalcommons.wku.edu/theses/1505.

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AtPAP15 is one of the purple acid phosphatases expressed by Arabidopsis thaliana that has been extensively studied. Purified AtPAP15 has been shown to exhibit both phytase and phosphomonoesterase activities in acidic pH with maximal activity at pH 4.5. AtPAP15 is a phosphorus starvation inducible (PSI) gene that is expressed highly during phosphorus deficient conditions. In the current study, AtPAP15 was overexpressed in Nicotiana tabaccum under cauliflower mosaic virus (CaMV35S) constitutive promoter. After PCR confirmation of the gene, plants were transferred to the greenhouse and allowed to
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47

Cupit, Pauline M. "Cloning and expression of a human type V acid phosphatase single chain antibody fragment in Escherichia coli and Pichia pastoris." Thesis, University of Aberdeen, 1996. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU078319.

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A recombinant single chain antibody (scAb) fragment specific for the isoenzyme human type V acid phosphatase has been expressed and secreted from Escherichia coli at a level of 0.02 mg/litre culture volume. Purification of the fragment using antibody immunoaffinity and metal chelate affinity chromatography yielded a mixture of dimer and monomer. The binding sensitivity of the dimeric scAb was ten fold lower than that of the parent monoclonal antibody as determined by BIAcore and ELISA. The methylotrophic yeast Pichia pastoris was used for high level expression of the scAb fragment. Using the P
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SOUZA, Amanda Araujo. "Produção e caracterização bioquímica de uma fosfatase ácida de Trichoderma harzianum (ALL42)." Universidade Federal de Goiás, 2011. http://repositorio.bc.ufg.br/tede/handle/tde/1267.

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Made available in DSpace on 2014-07-29T15:16:32Z (GMT). No. of bitstreams: 1 dissertacao de mestrado completa Amanda A Souza.pdf: 994299 bytes, checksum: 8cf098dd70299abaf08a216436f38888 (MD5) Previous issue date: 2011-06-29<br>Trichoderma harzianum is a saprophytic fungus, known for its potential as a biological control agent of different phytopathogens that causes losses in crops. Its action is based on different mechanisms like volatile and non-volatile antibiotics production, competition for nutrient and space, production of hydrolytic enzymes and mycoparasitism. This fungus also plays
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Leelasart, Bundit. "Étude sur l'excrétion de la phosphatase acide extracytoplasmique de levures : Rhodotorula glutinis 3044 et saccharomyces uvarum 0006." Nancy 1, 1987. http://www.theses.fr/1987NAN10202.

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Le PH du milieu module l'excrétion de la phosphatase acide de rhodotorula glutinis. Deux formes de phosphatase acide (liée et libérée) ont un PH optimal compris entre 2 et 5,5. Ce sont des phosphomonoestérases ayant une spécificité identique pour divers substrats. La phosphatase acide extracytoplasmique a été purifiée, c'est une glycoprotéine de masse moléculaire 300 KDA, formées de sous-unité de PM=97 KDA, et la partie glucidique est essentiellement constituée de mannose, et la partie protéique riche en résidu aspériagine, threonine, serine, glutamine, alunine et leucine
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Marchesano, Luiz Henrique [UNESP]. "Comportamento de marcadores séricos de formação e reabsorção óssea após enxerto autógeno em fissura alveolar congênita: sem e com plasma rico em plaquetas." Universidade Estadual Paulista (UNESP), 2005. http://hdl.handle.net/11449/100132.

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Made available in DSpace on 2014-06-11T19:30:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-12-06Bitstream added on 2014-06-13T18:40:39Z : No. of bitstreams: 1 marchesano_lh_dr_arafcf.pdf: 336354 bytes, checksum: 54de5ce8ee681faefedf0567be191430 (MD5)<br>Universidade Estadual Paulista (UNESP)<br>O tratamento cirúrgico da fissura congênita do processo alveolar superior compreende o enxerto ósseo, um procedimento bem aceito e de grande importância na restauração da forma e da função perdidas. Associado ao enxerto ósseo tem-se utilizado um produto atóxico, não imunoreativo e de fácil
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