Relevant bibliographies by topics / Adsorption column chromatography

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Adsorption column chromatography'

Author: Grafiati
Published: 4 June 2021
Last updated: 13 February 2022

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Journal articles on the topic "Adsorption column chromatography"

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Firestone, David. "Determination of Dioxins and Furans in Foods and Biological Tissues: Review and Update." Journal of AOAC INTERNATIONAL 74, no. 2 (1991): 375–84. http://dx.doi.org/10.1093/jaoac/74.2.375.

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Abstract Determination of trace residues of polychlorlnated dlbenzo- p-dloxlns and dibenzofurans (PCDDs and PCDFs) In various matrixes Is carried out by a limited number of laboratories In the United States, Canada, and other countries. Current methods for analysis of foods and biological tissues Include a combination of preparation, extraction, cleanup, isolation, determination, and Identity confirmation procedures. Soxhlet, liquid/liquid, solid-phase, and column extraction procedures are used as well as treatment with acid or base before solvent extraction. Cleanup and isolation steps Includ
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Eltekov, Anton Yu. "Effect of temperature on adsorption and chromatography of polystyrene macromolecules." Сорбционные и хроматографические процессы 18, no. 6 (2018): 810–15. http://dx.doi.org/10.17308/sorpchrom.2018.18/608.

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Study of the effect of temperature on the parameters of adsorption and liquid chromatography of
 molecules and macromolecules constantly attracts the attention of specialists. The potential benefits of ele- vated temperature column, particularly enhanced kinetic and transport properties, which are based on the reduction of mobile phase viscosity and increase the diffusion of analyte at high temperature, began to be actively used for the rapid analysis of molecules and macromolecules by chromatographic methods in recent years. It is now recognized that temperature is an important tool to o
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Jandera, Pavel, and Tomáš Hájek. "A New Definition of the Stationary Phase Volume in Mixed-Mode Chromatographic Columns in Hydrophilic Liquid Chromatography." Molecules 26, no. 16 (2021): 4819. http://dx.doi.org/10.3390/molecules26164819.

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Polar columns used in the HILIC (Hydrophilic Interaction Liquid Chromatography) systems take up water from the mixed aqueous–organic mobile phases in excess of the water concentration in the bulk mobile phase. The adsorbed water forms a diffuse layer, which becomes a part of the HILIC stationary phase and plays dominant role in the retention of polar compounds. It is difficult to fix the exact boundary between the diffuse stationary and the bulk mobile phase, hence determining the column hold-up volume is subject to errors. Adopting a convention that presumes that the volume of the adsorbed wa
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Nasuto, R. "Relationship between Stationary and Mobile Phase Composition and its Influence on the Retention Time of Some Analytes in Gas Chromatography." Adsorption Science & Technology 18, no. 4 (2000): 323–31. http://dx.doi.org/10.1260/0263617001493468.

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A binary methanol vapour/hydrogen gas mixture has been used as the mobile phase in gas chromatography. Through the use of modified frontal analysis (undertaken just before measurements of the retention of the tested analytes), it has been possible to determine the adsorption isotherm of the mobile phase modifier (methanol) under typical conditions for a chromatographic process. It was found that adsorption of the mobile phase modifier on the column packing surface caused a decrease in the retention times of all the analytes tested. Furthermore, as a result of such adsorption, an increase in th
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Krupčík, Ján, Ivan Skačáni, Eva Benická, and Pat Sandra. "Dependence of Gas Chromatographic Retention Data of Hydrocarbons on the Film Thickness of the Polydimethylsiloxane Stationary Phase." Collection of Czechoslovak Chemical Communications 59, no. 11 (1994): 2390–96. http://dx.doi.org/10.1135/cccc19942390.

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Kovats indices of cyclic and aromatic hydrocarbons, separated by capillary gas liquid chromatography on polydimethylsiloxane capillary columns, were found to increase with increasing stationary phase film thickness. This effect is explained in terms of adsorption of the stationary phase on the active sites of the inner surface of the capillary column. Since the number of active sites is limited, the overall polarity of the polydimethylsiloxane stationary phase is better defined in columns with thick stationary phase films. Interlaboratory reproducibility of retention indices of cyclic and arom
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Larin, A. V. "Criterion for the Quantitative Assessment of Ideal Conditions in Chromatography." Adsorption Science & Technology 6, no. 4 (1989): 212–18. http://dx.doi.org/10.1177/026361748900600404.

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The quantitative relationship between the plate theory variant model of equilibrium adsorption in which time is the only independent variable and the theory of ideal chromatogaphy has been evaluated. It has been shown that an approach to ideal conditions depends directly on an increase in the relative (or absolute) length of the column. Practical recommendations on the choice and assessment of conditions to verify the measurement of adsorption isotherms and on the calculation of chromatographic processes in accordance with the theory of ideal chromatography are given.
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Contreras-Larios, José L., Antonia Infantes-Molina, Luís A. Negrete-Melo, et al. "Separation of N–C5H12–C9H20 Paraffins Using Boehmite by Inverse Gas Chromatography." Applied Sciences 9, no. 9 (2019): 1810. http://dx.doi.org/10.3390/app9091810.

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The separation of a mixture of C5–C9 n-paraffins was achieved by Inverse Gas Chromatography (IGC) by using boehmite; AlO(OH), in a packed column with short exposure times and temperatures; from 45 °C to 52 °C. The boehmite was characterized by XRD; ATG; SEM; IR spectroscopy and N2 adsorption. The material exhibited a low crystalline boehmite (AlOOH) structure and presented high hydration (pseudoboehmite). The reverse gas chromatography measurements showed that the elution temperatures of the C5–C9 n-paraffins were low compared with those obtained for other adsorbents. The differential heat of
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Li, Xia, Ai Ling Du, Ai Qin Du, and Yan Wang. "Isolation of Oxymatrine from Alkaloids of Sophora Flavescens Ait. by H103 Macroporous Resin." Advanced Materials Research 634-638 (January 2013): 403–7. http://dx.doi.org/10.4028/www.scientific.net/amr.634-638.403.

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An efficient and environment-friendly macroporous resin column chromatographic separation method has been developed for preparative isolation of oxymatrine from alkaloids of Sophora flavescens Ait. in the present study. The static adsorption ratios of nine macroporous resins were evaluated. The results demonstrate that H103 macroporous resin, showing high adsorption and desorption capacity, is suitable for separation of oxymatrine. The optimal parameters are obtained: the H103 resins are packed with ethanol, height-diameter ratio (H/D in short) of H103 resin-packed chromatogram column is 33:1,
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ZHAI, Xinlei, Jinguang XU, Xiufeng XU, et al. "Preparation of Supported Nano-gold Catalyst by Adsorption Column Chromatography." CHINESE JOURNAL OF CATALYSIS (CHINESE VERSION) 32, no. 2 (2011): 374–78. http://dx.doi.org/10.3724/sp.j.1088.2011.01004.

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YAMAMOTO, Yojiro. "Compositional analysis of heavy oils by adsorption column chromatography-gravimetry." Bunseki kagaku 35, no. 1 (1986): 18–22. http://dx.doi.org/10.2116/bunsekikagaku.35.18.

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Dissertations / Theses on the topic "Adsorption column chromatography"

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Samuelsson, Jörgen. "Development of Methods for Phase System Characterization in Liquid Chromatography." Doctoral thesis, Uppsala universitet, Ytbioteknik, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8597.

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The aim of this thesis is first and foremost to improve the fundamental knowledge of nonlinear and preparative separation theory by focusing on some of the remaining “white spots” on the theoretical chromatographic map. Secondly, the acquired knowledge is used to develop, validate and execute new methods for phase characterization in liquid chromatography. The methodology used in this thesis is a combination of experiments, fundamental nonlinear theory and systematic computer simulations. A fundamental knowledge of the molecular interactions between the compounds to be separated and the separa
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Soukupová, Alena. "Stanovení musk sloučenin v biotických matricích." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2011. http://www.nusl.cz/ntk/nusl-216690.

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This diploma thesis deals with actual issues of the contamination in the environment with synthetic fragrances. Musk compounds are infiltrated to many environmental components (especially an aquatic ecosystem) because of their biological persistence and the ability of accumulation. This diploma thesis is focused on the selection and the optimization of method for the determination of musk compounds in real biotic matrices (fish). The isolation of analytes was realized by PSE method and the purification of extract was realized by the method of the adsorption column chromatography. Identificatio
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Monaco, Enzo. "pH Transients in Hydroxyapatite chromatography columns." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2018.

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Ceramic Hydroxyapatite (CHT), with empirical formula (Ca5(PO4)3OH)2, is a material used as ion exchange resin in chromatographic applications. The material, having both positive and negative charged sites, can be used in many different contexts encountered in the protein purification processes. Nevertheless, the resin shows an intrinsic limitation for this kind of applications: even if the material solubility in water is very low at pH values higher than 6.5, it sharply increases in more acidic environments, reducing the life of the material and increasing the operative costs of the process, m
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Urio, Ricardo de Prá. "Cromatografia a líquido por injeção sequencial para a determinação de herbicidas triazínicos e metabólitos da atrazina explorando o uso de cela de longo caminho óptico e monitoramento on-line em estudos de adsorção." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-31082011-143740/.

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Estudou-se o emprego da Cromatografia a Líquido por Injeção Seqüencial (SIC) explorando o uso de uma cela de longo caminho óptico com guia de onda (LCW) de 100 cm para a melhora dos limites de detecção (LOD) e quantificação (LOQ) na determinação de atrazina (ATR), propazina (PRO) e simazina (SIM). Para isto, utilizou-se uma fase móvel com composição de 44:56 (v v-1) metanol : tampão acetato de amônio 1,25 mM, pH 4,7, coluna monolítica e a detecção espectrofotométrica em 238 nm. Obtiveram-se valores de LOD e LOQ, respectivamente, de 1,76 e 5,86 µg L-1 para ATR, 4,51 e 15 µg L-1 para P
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Zhang, Runtong. "Measurement of effective diffusivity : chromatographic method (pellets & monoliths)." Thesis, University of Bath, 2013. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608352.

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This thesis aims to find out the effective diffusivity (Deff) of a porous material – γ-alumina, using an unsteady state method with two inert gases at ambient condition with no reactions. For porous materials, Deff is important because it determines the amount of reactants that transfers to the surface of pores. When Deff is known, the apparent tortuosity factor of γ-alumina is calculated using the parallel pore model. The apparent tortuosity factor is important because: (a) it can be used to back-calculate Deff at reacting conditions; (b) once Deff with reactions is known, the Thiele modulus
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Garcia, Ausberta Jesús Cabezas. "Metodologia analítica para determinação de triclosan e clorofenois por cromatografia a líquido de alta eficiência (HPLC) e cromatografia por injeção seqüencial (SIC) com uso de coluna monolítica e empacotada." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-28032012-112652/.

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Foram desenvolvidas metodologias de cromatografia a líquido de fase reversa baseadas em injeção sequencial (SIC) e em cromatografia a líquido de alta eficiência (HPLC) para determinação de triclosan em amostras de produtos de higiene pessoal e em estudos de adsorção em argilominerais naturais e modificados, visando determinar parâmetros de adsorção de triclosan frente a alguns de seus metabólitos. A determinação de triclosan em enxaguadores bucais foi realizada por SIC com eluição isocrática usando fase móvel constituída por acetonitrila: tampão fosfato de trietilamina 70 mM pH 3,5 na proporçã
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Chang, Hui-Lan, and 張慧蘭. "The Study of Protein Purification in Adsorption Column Chromatography by Back Flush." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/45524028481665858296.

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碩士<br>淡江大學<br>化學學系碩士班<br>100<br>Plasmids carrying YNR029Cp or ALD4p gene were transformed into E.coli BL21(DE3), and the individual protein was then expressed and purified. Q-Sepharose Fast Flow, Hydroxylapatite (for purified YNR029Cp), Phenyl Sepharose 6 Fast Flow, Blue Sepharose 6 Fast Flow (for over-expressed ALD4p) were employed in order to reversely adsorb the purified protein sample, followed by forward elution of the protein. With the different pH and flow rate, we tried to identify and test the relationship to the order of elution and the separation conditions.
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Sridhar, P. "Analysis Of Protein Purification By Affinity Chromatography." Thesis, 1997. http://etd.iisc.ernet.in/handle/2005/1781.

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Book chapters on the topic "Adsorption column chromatography"

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Bruce, L. J., S. Ghose, and H. A. Chase. "The effect of column verticality on separation efficiency in expanded bed adsorption." In Expanded Bed Chromatography. Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-017-1519-5_6.

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Mattiasson, Bo, and M. P. Nandakumar. "Binding assays in heterogeneous media using a flow injection system with an expanded micro-bed adsorption column." In Expanded Bed Chromatography. Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-017-1519-5_26.

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Barker, P. E., and G. Ganetsos. "Biochemical Reaction and Separation in Chromatographic Columns." In Adsorption: Science and Technology. Springer Netherlands, 1989. http://dx.doi.org/10.1007/978-94-009-2263-1_25.

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Cowan, G. H. "Development of Physical and Mathematical Modelling Methods for Scale-Up of Batch Stirred Tank and Packed-Bed Column Adsorption and Chromatographic Units." In Adsorption: Science and Technology. Springer Netherlands, 1989. http://dx.doi.org/10.1007/978-94-009-2263-1_27.

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Hayes, T. K., A. A. Strey, and K. W. Beyenbach. "Adsorption Chromatography of Small C-Terminal Peptide Amides on Dihydroxyalkyl Bonded Silica High Performance Liquid Chromatography Columns and Application to Purification of Insect Neuropeptides." In Chromatography and Isolation of Insect Hormones and Pheromones. Springer US, 1990. http://dx.doi.org/10.1007/978-1-4684-8062-7_20.

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"Column Chromatography (adsorption chromatography)." In Encyclopedia of Genetics, Genomics, Proteomics and Informatics. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6754-9_3367.

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Harrison, Roger G., Paul W. Todd, Scott R. Rudge, and Demetri P. Petrides. "Liquid Chromatography and Adsorption." In Bioseparations Science and Engineering. Oxford University Press, 2015. http://dx.doi.org/10.1093/oso/9780195391817.003.0010.

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Liquid chromatography and adsorption processes are based on the differential affinity of various soluble molecules for specific types of solids. In these processes, equilibrium is approached between a solid phase, often called the resin, or stationary phase, and the soluble molecules in a liquid phase. The solid phase is “stationary” because it is often packed in a fixed column. Since the liquid phase is often flowing past the solid phase, it is referred to as the mobile phase. Chromatography and adsorption are related unit operations. In chromatography, typically multiple solutes are separated from each other, with the target product solute being one of many that might be recovered at the end of the process step. In adsorption, there are typically only three groups of solutes: those that do not adsorb to the stationary phase (sometimes called “flow through”); secondly, those that adsorb and then are subsequently recovered by an elution step; and thirdly, those solutes that are nearly irreversibly bound and can only be removed from the adsor­bent by regenerating the adsorbent, which usually results in the chemical destruction of these solutes. The word “adsorption” is used both to describe the physical adherence of a solute to a stationary phase, and as the name of the unit operation described above. Adsorption is a subset of the “sorption” phenomena, absorption (transfer of a solute from one phase into another) and ion exchange (exchange of a counter-ion between two opposing co-ions) being the other two sorption phenomena. The unit operations chromatography and adsorption can rely on any of these three sorption processes individually or in combination. In chromatography and adsorption, a mixture of solutes in a feed solution is introduced at the inlet of a column containing the stationary phase and separated into zones of individual solutes over the length of the column. The solutes are carried by the convective action of an elution solvent that is continuously fed to the column after the feed solution has been introduced.
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Chimowitz, Eldred H. "Supercritical Adsorption." In Introduction to Critical Phenomena in Fluids. Oxford University Press, 2005. http://dx.doi.org/10.1093/oso/9780195119305.003.0008.

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In this chapter, we discuss adsorption phenomena in supercritical systems, a situation that occurs in many application areas in chemical-process and materials engineering. An example of a commercial application in this area, which has achieved wide acceptance as a tool in analytical chemistry, is supercritical fluid chromatography (SFC). Not only is SFC a powerful technique for chemical analysis, but it also is a useful method for measuring transportive and thermodynamic properties in the near-critical systems. In the next section, we analyze adsorption-column dynamics using simple dynamic models, and describe how data from a chromatographic column can be used to estimate various thermodynamic and transport properties.We then proceed to discuss the effects of proximity to the critical point on adsorption behavior in these systems. The closer the system is to its critical point, the more interesting is its behavior. For very dilute solute systems, like those considered here, the energy balance is often ignored to a first approximation; this leads to a simple set of mass-balance equations defining transport for each species. These equations can be developed to various levels of complexity, depending upon the treatment of the adsorbent (stationary phase). The conceptual view of these phases can span a wide range of possibilities ranging from completely nonporous solids (fused structures) to porous materials with complicated ill-defined pore structures. Given these considerations, it is customary to make the following assumptions in the development of a simple model of adsorber-bed dynamics: . . .1. The stationary and mobile phases are continuous in the direction of the flow, with the fluid phase possessing a flat velocity profile (“plug” flow).. . . . . . 2. The porosity of the stationary phase is considered constant irrespective of pressure and temperature conditions (i.e., it is incompressible). . . . . . .3. The column is considered to be radially homogeneous, leading to a set of equations with one spatially independent variable, representing distance along the column axis. . . . . . . 4. The dispersion term in the model equation represents the combined effects of molecular diffusion and dispersion due to convective stirring in the bed. These effects are combined into an effective phenomenological dispersion coefficient, considered to be constant throughout the column. . . .
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Cutler, P. "Chromatography on the basis of size." In Protein Purification Techniques. Oxford University Press, 2001. http://dx.doi.org/10.1093/oso/9780199636747.003.0012.

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Chromatography has been employed for the separation of proteins and other biological macromolecules on the basis of molecular size since the mid 1950s when Lathe and Rutheven employed modified starch as a media for separation. Porath and Flodin developed the technique further using cross-linked dextran and coined the term gel filtration. Some confusion over nomenclature has been created by the term gel permeation, used to describe separation by the same principle in organic mobile phases using synthetic matrices. It is now generally agreed that the terms gel filtration and gel permeation do not accurately reflect the nature of the separation. Size exclusion Chromatography (SEC) has been widely accepted as a universal description of the technique and in line with the IUPAC nomenclature this term will be adopted. The historical development of SEC for protein separation has been reviewed. SEC is a commonly used technique due to the diversity of the molecular sizes of proteins in biological tissues and extracts. In addition to isolating proteins from crude mixtures, SEC has been employed for many roles including buffer exchange (desalting), removal of non-protein contaminants (DNA, viruses), protein aggregate removal, the study of biological interactions, and protein folding. The principle of size exclusion is based on a solid phase matrix consisting of beads of defined porosity which are packed into a column through which a mobile liquid phase flows. The mobile phase has access to both the volume inside the pores and the volume external to the beads. Unlike many other chromatographic procedures size exclusion is not an adsorption technique. Separation can be visualized as reversible partitioning into the two liquid volumes. The elution time is dependent upon an individual protein’s ability to access the pores of the matrix. Large molecules remain in the volume external to the beads as they are unable to enter the pores. The resulting shorter flow path means that they pass through the column relatively rapidly, emerging early. Proteins that are excluded from the pores completely, elute in the void volume, V0. This is often determined experimentally by the use of a high molecular weight component such as blue dextran or calf thymus DNA.
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Harris, E. L. V. "Concentration of the extract." In Protein Purification Techniques. Oxford University Press, 2001. http://dx.doi.org/10.1093/oso/9780199636747.003.0010.

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A concentration step is frequently required after a clarified solution of the protein has been obtained, in order to aid subsequent purification steps. This is particularly important when the protein is obtained in culture medium from cells (e.g. bacteria or tissue culture cells). Concentration of the protein solution results in a decreased volume, as well as a higher protein concentration. Clearly a smaller volume of solution is easier to handle in subsequent steps, such as precipitation or loading onto a chromatography column. Higher protein concentration minimize protein losses by non-specific adsorption to container walls or column matrices. In addition many subsequent purification steps require a minimum protein concentration to be effective, for example, precipitation is more efficient at concentrations above 100 μg/ml, whilst for adsorption chromatography (e.g. ion exchange or affinity) the concentration of protein must be greater than the dissociation constant. Concentration is achieved by removal of water and other small molecules: (a) By addition of a dry matrix polymer with pores that are too small to allow entry of the large protein molecules (Section 2). (b) By removal of the small molecules through a semi-permeable membrane which will not allow the large molecules through (i.e. ultrafiltration, Section 3). (c) By removal of water in vacua (i.e. lyophilization, Section 4). Precipitation can also be used to concentrate proteins if the pellet is redissolved in a smaller volume, and in addition often results in some degree of purification of the protein of interest. However, as mentioned above precipitation is more effective if the total protein concentration is above 100 μg/ml (see Section 6). Two-phase aqueous extraction can also be used to concentrate the protein, with an associated degree of purification (see Section 7). This is one of the simplest and quickest methods of concentrating solutions of proteins, requiring minimal apparatus. A dry matrix polymer, such as Sephadex, is added to the protein solution and allowed to absorb the water and other small molecules; the pores within the matrix are too small to allow the protein to be absorbed.
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Conference papers on the topic "Adsorption column chromatography"

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Li-ling, Yang, Yang Hong-guang, Liu Zhen-xing, and Xia Ti-rui. "Analysis and Application of Trace H2 and CH4 in Cycling Process Gas of Fusion Reactors by Gas Chromatography." In 2017 25th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/icone25-67522.

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It’s necessary to establish a high-precision analytical method of Gas Chromatography (GC) for achieving the rapid detection of trace hydrogen and methane in cycling process gas of fusion reactors. The analysis of H2, CH4 with concentrations from 0.5ppm to 5000ppm were detected through the application of Discharge Ionization Detector (DID) in high purity helium and the column of molecular sieve, whose parameters was 15m×0.53mm×50μm. The studies have shown that the retention time of H2, CH4 were 36s and 71s, respectively, their relatively standard deviation (RSD) of area responses were severally
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Riethorst, W., M. W. P. M. te Booy, T. Beugeling, A. Bantjes, J. Over, and W. G. van Aken. "THE ISOLATION OF COAGULATION FACTOR VIII FROM HUMAN BLOOD PLASMA BY AFFINITY CHROMATOGRAPHY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644059.

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The need for high quality concentrates of coagulation factor VIII (FVIII:C) for treatment of haemophilia A is increasing. As the purity of FVIII:C obtained with existing large scale methods is poor and yields are low, another method for the isolation of FVIII is being developed primarily to avoid losses incurred during cryoprecipitation.Affinity gels were prepared by derivatizing Sepharose CL 4B with different positively charged ligand-spacer combinations. The adsorption of FVIII as well as the von Willebrand factor (VWF) from human blood plasma onto these gels was measured by a one-stage assa
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Takeya, H., S. Kawabata, T. Miyata, T. Morita, and S. Iwanaga. "A MODIFIED METHOD FOR PURIFICATION OF BOVINE FACTOR VII AND ITS PRIMARY STRUCTURE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643785.

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A modified method for purification of factor VII from bovine plasma was developed. The isolation procedures consisted of four steps with the ordinary barium citrate adsorption (1), DEAE-Sepharose chromatography (2), twice chromatographies on a benzamidine-Sepharose column (3) and affinity chromatography on Affigel-10 coupled with staphylocoagulase produced by Staphylococcus aureus, strain 213 (4). This final step was very effective for the removal of prothrombin contained in the pooled fraction of factor VII. Thus, these procedures allowed higher recovery of factor VII than the earlier methods
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