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Journal articles on the topic 'Affinity sensor'

Author: Grafiati

Published: 3 June 2023

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1

Qian, Xiang, Xiaowei Niu, and Karl L. Magleby. "Intra- and Intersubunit Cooperativity in Activation of BK Channels by Ca2+." Journal of General Physiology 128, no. 4 (2006): 389–404. http://dx.doi.org/10.1085/jgp.200609486.

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The activation of BK channels by Ca2+ is highly cooperative, with small changes in intracellular Ca2+ concentration having large effects on open probability (Po). Here we examine the mechanism of cooperative activation of BK channels by Ca2+. Each of the four subunits of BK channels has a large intracellular COOH terminus with two different high-affinity Ca2+ sensors: an RCK1 sensor (D362/D367) located on the RCK1 (regulator of conductance of K+) domain and a Ca-bowl sensor located on or after the RCK2 domain. To determine interactions among these Ca2+ sensors, we examine channels with eight d

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2

Tlili, Chaker, Sushmee Badhulika, Thien-Toan Tran, Ilkeun Lee, and Ashok Mulchandani. "Affinity chemiresistor sensor for sugars." Talanta 128 (October 2014): 473–79. http://dx.doi.org/10.1016/j.talanta.2014.05.055.

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3

Glad, Cristina, Karin Sjödin, and Bo Mattiasson. "Streaming potential—a general affinity sensor." Biosensors 2, no. 2 (1986): 89–100. http://dx.doi.org/10.1016/0265-928x(86)80012-8.

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4

Huang, Xian, Charles Leduc, Yann Ravussin, et al. "A differential dielectric affinity glucose sensor." Lab Chip 14, no. 2 (2014): 294–301. http://dx.doi.org/10.1039/c3lc51026c.

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Labouesse, Marie A., Reto B. Cola, and Tommaso Patriarchi. "GPCR-Based Dopamine Sensors—A Detailed Guide to Inform Sensor Choice for In Vivo Imaging." International Journal of Molecular Sciences 21, no. 21 (2020): 8048. http://dx.doi.org/10.3390/ijms21218048.

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Understanding how dopamine (DA) encodes behavior depends on technologies that can reliably monitor DA release in freely-behaving animals. Recently, red and green genetically encoded sensors for DA (dLight, GRAB-DA) were developed and now provide the ability to track release dynamics at a subsecond resolution, with submicromolar affinity and high molecular specificity. Combined with rapid developments in in vivo imaging, these sensors have the potential to transform the field of DA sensing and DA-based drug discovery. When implementing these tools in the laboratory, it is important to consider

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6

Efremenko, Yulia, and Vladimir M. Mirsky. "Electrical Control of the Receptor Affinity." Engineering Proceedings 6, no. 1 (2021): 3. http://dx.doi.org/10.3390/i3s2021dresden-10084.

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A concept of virtual sensor array based on an electrically controlled variation of affinity properties of the receptor layer was realized on the base of integrated electrochemical chemotransistor containing conducting polymer as the receptor layer. Electrical control of the redox-state of the polymer (polyaniline) was performed in a five-electrode configuration with four electrodes for conductivity measurements and Ag/AgCl reference electrode integrated on the same glass chip. An ionic liquid provided an electrical connection between the reference electrode and chemosensitive material. Conduct

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Yin, Ruixue, Jizhong Xin, Dasheng Yang, et al. "High-Linearity Hydrogel-Based Capacitive Sensor Based on Con A–Sugar Affinity and Low-Melting-Point Metal." Polymers 14, no. 20 (2022): 4302. http://dx.doi.org/10.3390/polym14204302.

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Continuous glucose monitoring (CGM) plays an important role in the treatment of diabetes. Affinity sensing based on the principle of reversible binding to glucose does not produce intermediates, and the specificity of concanavalin A (Con A) to glucose molecules helps to improve the anti-interference performance and long-term stability of CGM sensors. However, these affinity glucose sensors have some limitations in their linearity with a large detection range, and stable attachment of hydrogels to sensor electrodes is also challenging. In this study, a capacitive glucose sensor with high linear

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Ramanavicius, Simonas, Arunas Jagminas, and Arunas Ramanavicius. "Advances in Molecularly Imprinted Polymers Based Affinity Sensors (Review)." Polymers 13, no. 6 (2021): 974. http://dx.doi.org/10.3390/polym13060974.

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Recent challenges in biomedical diagnostics show that the development of rapid affinity sensors is very important issue. Therefore, in this review we are aiming to outline the most important directions of affinity sensors where polymer-based semiconducting materials are applied. Progress in formation and development of such materials is overviewed and discussed. Some applicability aspects of conducting polymers in the design of affinity sensors are presented. The main attention is focused on bioanalytical application of conducting polymers such as polypyrrole, polyaniline, polythiophene and po

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9

Tuccitto, Nunzio, Luca Spitaleri, Giovanni Li Destri, Andrea Pappalardo, Antonino Gulino, and Giuseppe Trusso Sfrazzetto. "Supramolecular Sensing of a Chemical Warfare Agents Simulant by Functionalized Carbon Nanoparticles." Molecules 25, no. 23 (2020): 5731. http://dx.doi.org/10.3390/molecules25235731.

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Real-time sensing of chemical warfare agents by optical sensors is today a crucial target to prevent terroristic attacks by chemical weapons. Here the synthesis, characterization and detection properties of a new sensor, based on covalently functionalized carbon nanoparticles, are reported. This nanosensor exploits noncovalent interactions, in particular hydrogen bonds, to detect DMMP, a simulant of nerve agents. The nanostructure of the sensor combined with the supramolecular sensing approach leads to high binding constant affinity, high selectivity and the possibility to reuse the sensor.

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10

Brown, Victoria, Jessica A. Sexton, and Mark Johnston. "A Glucose Sensor in Candida albicans." Eukaryotic Cell 5, no. 10 (2006): 1726–37. http://dx.doi.org/10.1128/ec.00186-06.

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ABSTRACT The Hgt4 protein of Candida albicans (orf19.5962) is orthologous to the Snf3 and Rgt2 glucose sensors of Saccharomyces cerevisiae that govern sugar acquisition by regulating the expression of genes encoding hexose transporters. We found that HGT4 is required for glucose induction of the expression of HGT12, HXT10, and HGT7, which encode apparent hexose transporters in C. albicans. An hgt4Δ mutant is defective for growth on fermentable sugars, which is consistent with the idea that Hgt4 is a sensor of glucose and similar sugars. Hgt4 appears to be sensitive to glucose levels similar to

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11

Shahrezaei, Vahid, and Kerry R. Delaney. "Brevity of the Ca2+ Microdomain and Active Zone Geometry Prevent Ca2+-Sensor Saturation for Neurotransmitter Release." Journal of Neurophysiology 94, no. 3 (2005): 1912–19. http://dx.doi.org/10.1152/jn.00256.2005.

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The brief time course of the calcium (Ca2+) channel opening combined with the molecular-level colocalization of Ca2+ channels and synaptic vesicles in presynaptic terminals predict sub-millisecond calcium concentration ([Ca2+]) transients of ≥100 μM in the immediate vicinity of the vesicle. This [Ca2+] is much higher than some of the recent estimates for the equilibrium dissociation constant of the Ca2+ sensor(s) that control neurotransmitter release, suggesting release should be close to saturation, yet it is well known that release is highly sensitive to changes in Ca2+ influx. We show that

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12

Trull, Keelan J., Piper Miller, Kiet Tat, S. Ashley Varney, Jason M. Conley, and Mathew Tantama. "Detection of Osmotic Shock-Induced Extracellular Nucleotide Release with a Genetically Encoded Fluorescent Sensor of ADP and ATP." Sensors 19, no. 15 (2019): 3253. http://dx.doi.org/10.3390/s19153253.

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Purinergic signals, such as extracellular adenosine triphosphate (ATP) and adenosine diphosphate (ADP), mediate intercellular communication and stress responses throughout mammalian tissues, but the dynamics of their release and clearance are still not well understood. Although physiochemical methods provide important insight into physiology, genetically encoded optical sensors have proven particularly powerful in the quantification of signaling in live specimens. Indeed, genetically encoded luminescent and fluorescent sensors provide new insights into ATP-mediated purinergic signaling. Howeve

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13

Schuderer, J., A. Akkoyun, A. Brandenburg, U. Bilitewski, and E. Wagner. "Development of a Multichannel Fluorescence Affinity Sensor System." Analytical Chemistry 72, no. 16 (2000): 3942–48. http://dx.doi.org/10.1021/ac000222f.

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14

Falter, J., R. Medina, and H. L. Schmidt. "Concepts of artifical affinity systems for sensor development." Sensors and Actuators B: Chemical 19, no. 1-3 (1994): 694–97. http://dx.doi.org/10.1016/0925-4005(93)01132-n.

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15

Xia, Haiyang, Song Zha, Jijun Huang, and Jibin Liu. "Radio environment map construction by adaptive ordinary Kriging algorithm based on affinity propagation clustering." International Journal of Distributed Sensor Networks 16, no. 5 (2020): 155014772092248. http://dx.doi.org/10.1177/1550147720922484.

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In the era of 5G mobile communication, radio environment maps are increasingly viewed as a powerful weapon for the optimization of spectrum resources, especially in the field of autonomous vehicles. However, due to the constraint of limited resources when it comes to sensor networks, it is crucial to select a suitable scale of sensor measurements for radio environment map construction. This article proposes an adaptive ordinary Kriging algorithm based on affinity propagation clustering as a novel spatial interpolation method for the construction of the radio environment map, which can provide

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16

Bradberry, Mazdak M., Huan Bao, Xiaochu Lou та Edwin R. Chapman. "Phosphatidylinositol 4,5-bisphosphate drives Ca2+-independent membrane penetration by the tandem C2 domain proteins synaptotagmin-1 and Doc2β". Journal of Biological Chemistry 294, № 28 (2019): 10942–53. http://dx.doi.org/10.1074/jbc.ra119.007929.

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Exocytosis mediates the release of neurotransmitters and hormones from neurons and neuroendocrine cells. Tandem C2 domain proteins in the synaptotagmin (syt) and double C2 domain (Doc2) families regulate exocytotic membrane fusion via direct interactions with Ca2+ and phospholipid bilayers. Syt1 is a fast-acting, low-affinity Ca2+ sensor that penetrates membranes upon binding Ca2+ to trigger synchronous vesicle fusion. The closely related Doc2β is a slow-acting, high-affinity Ca2+ sensor that triggers spontaneous and asynchronous vesicle fusion, but whether it also penetrates membranes is unkn

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17

Tay, Li-Lin, Shawn Poirier, Ali Ghaemi, and John Hulse. "Inkjet-printed paper-based surface enhanced Raman scattering (SERS) sensors for the detection of narcotics." MRS Advances 7, no. 9 (2022): 190–96. http://dx.doi.org/10.1557/s43580-022-00257-8.

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AbstractRecent advances in inkjet-printing of advanced materials have provided a versatile platform for the rapid development and prototyping of sensor devices. We have recently demonstrated inkjet-printed surface enhanced Raman scattering (SERS) sensors on flexible substrates for the detection of variety of small molecules [Tay et al. in Front Chem 9:680556 (2021); Tay et al. in J Raman Spectrosc 52:563 (2020)]. These flexible SERS sensors have many advantages for performing point-of-sampling testing, among them liquid or aerosol filtration and swabbing capabilities. These simple sampling and

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18

Yang, Wen, Jing Yu, Xiangtai Xi, et al. "Preparation of Graphene/ITO Nanorod Metamaterial/U-Bent-Annealing Fiber Sensor and DNA Biomolecule Detection." Nanomaterials 9, no. 8 (2019): 1154. http://dx.doi.org/10.3390/nano9081154.

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In this paper, a graphene/ITO nanorod metamaterial/U-bent-annealing (Gr/ITO-NM/U-bent-A)-based U-bent optical fiber local surface plasmon resonance (LSPR) sensor is presented and demonstrated for DNA detection. The proposed sensor, compared with other conventional sensors, exhibits higher sensitivity, lower cost, as well as better biological affinity and oxidize resistance. Besides, it has a structure of an original Indium Tin Oxides (ITO) nanocolumn array coated with graphene, allowing the sensor to exert significant bulk plasmon resonance effect. Moreover, for its discontinuous structure, a

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19

Thompson, Channing C., and Rebecca Y. Lai. "Threonine Phosphorylation of an Electrochemical Peptide-Based Sensor to Achieve Improved Uranyl Ion Binding Affinity." Biosensors 12, no. 11 (2022): 961. http://dx.doi.org/10.3390/bios12110961.

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We have successfully designed a uranyl ion (U(VI)-specific peptide and used it in the fabrication of an electrochemical sensor. The 12-amino acid peptide sequence, (n) DKDGDGYIpTAAE (c), originates from calmodulin, a Ca(II)-binding protein, and contains a phosphothreonine that enhances the sequence’s affinity for U(VI) over Ca(II). The sensing mechanism of this U(VI) sensor is similar to other electrochemical peptide-based sensors, which relies on the change in the flexibility of the peptide probe upon interacting with the target. The sensor was systematically characterized using alternating c

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20

Hilton, John P., Thai Huu Nguyen, Renjun Pei, Milan Stojanovic, and Qiao Lin. "A microfluidic affinity sensor for the detection of cocaine." Sensors and Actuators A: Physical 166, no. 2 (2011): 241–46. http://dx.doi.org/10.1016/j.sna.2009.12.006.

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21

Wannapob, Rodtichoti, Proespichaya Kanatharana, Warakorn Limbut, et al. "Affinity sensor using 3-aminophenylboronic acid for bacteria detection." Biosensors and Bioelectronics 26, no. 2 (2010): 357–64. http://dx.doi.org/10.1016/j.bios.2010.08.005.

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22

Schlatter, D., R. Barner, Ch Fattinger, et al. "The difference interferometer: application as a direct affinity sensor." Biosensors and Bioelectronics 8, no. 2 (1993): 109–16. http://dx.doi.org/10.1016/0956-5663(93)80059-x.

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23

Liebscher, Thilo, Franziska Glös, Andrea Böhme, et al. "Affinity Viscosimetry Sensor for Enzyme Free Detection of Glucose in a Micro-Bioreaction Chamber." Materials Science Forum 879 (November 2016): 1135–40. http://dx.doi.org/10.4028/www.scientific.net/msf.879.1135.

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With the growing demand of miniaturization of cell cultivation a new approach towards measuring and sensing bio-analytes needs to be made due to the problem of small volumes (less than 150μl) containing small amounts of analytes. Most of the available glucose sensors monitor the glucose concentration with the help of enzymes, which become very inaccurate in terms of long time measurement and uses (i.e. consumes) glucose during the measurement becoming not available anymore for the cells. Therefore, we focused on applying an enzyme-free glucose sensor based on a microelectromechanical system (M

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24

LIEBERZEIT, PETER A., ABDUL REHMAN, SADAF YAQUB, and FRANZ L. DICKERT. "NANOSTRUCTURED PARTICLES AND LAYERS FOR SENSING CONTAMINANTS IN AIR AND WATER." Nano 03, no. 04 (2008): 205–8. http://dx.doi.org/10.1142/s1793292008001015.

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Chemical sensor layers for environmental applications require optimal selectivity, sensitivity, and long term stability, which can be achieved in artificial matrices. For detecting thiols in air, reversible affinity interactions can be optimized by varying the stoichiometry of molybdenum disulphide nanoparticles to achieve sulphur deficiencies. Generating MoS1.9 increases the quartz crystal microbalance (QCM) sensor responses towards butane thiol by a factor of three. Artificial recognition sites are accessible by molecular imprinting: acrylate copolymers can be tuned in polarity to interact s

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25

Chang, Albert, Hsin-Yi Li, I.-Nan Chang, and Yen-Ho Chu. "Affinity Ionic Liquids for Chemoselective Gas Sensing." Molecules 23, no. 9 (2018): 2380. http://dx.doi.org/10.3390/molecules23092380.

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Selective gas sensing is of great importance for applications in health, safety, military, industry and environment. Many man-made and naturally occurring volatile organic compounds (VOCs) can harmfully affect human health or cause impairment to the environment. Gas analysis based on different principles has been developed to convert gaseous analytes into readable output signals. However, gas sensors such as metal-oxide semiconductors suffer from high operating temperatures that are impractical and therefore have limited its applications. The cost-effective quartz crystal microbalance (QCM) de

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Bian, Zhancun, Guiqian Fang, Ran Wang, Dongxue Zhan, Qingqiang Yao, and Zhongyu Wu. "A water-soluble boronic acid sensor for caffeic acid based on double sites recognition." RSC Advances 10, no. 47 (2020): 28148–56. http://dx.doi.org/10.1039/d0ra00980f.

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Herein, the specific recognition of caffeic acid by the double sites boronic acid sensor 5c is reported. The synergistic effect of the two recognition sites greatly improves the binding affinity and selectivity of the sensor.

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27

Bajaj, Aabha, Jakob Trimpert, Ibrahim Abdulhalim, and Zeynep Altintas. "Synthesis of Molecularly Imprinted Polymer Nanoparticles for SARS-CoV-2 Virus Detection Using Surface Plasmon Resonance." Chemosensors 10, no. 11 (2022): 459. http://dx.doi.org/10.3390/chemosensors10110459.

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COVID-19 caused by a SARS-CoV-2 infection was first reported from Wuhan, China, and later recognized as a pandemic on March 11, 2020, by the World Health Organization (WHO). Gold standard nucleic acid and molecular-based testing have largely satisfied the requirements of early diagnosis and management of this infectious disease; however, these techniques are expensive and not readily available for point-of-care (POC) applications. The COVID-19 pandemic of the 21st century has emphasized that medicine is in dire need of advanced, rapid, and cheap diagnostic tools. Herein, we report on molecular

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28

Chen, Ren-Shiang, Yanyan Geng, and Karl L. Magleby. "Mg2+ binding to open and closed states can activate BK channels provided that the voltage sensors are elevated." Journal of General Physiology 138, no. 6 (2011): 593–607. http://dx.doi.org/10.1085/jgp.201110707.

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BK channels are activated by intracellular Ca2+ and Mg2+ as well as by depolarization. Such activation is possible because each of the four subunits has two high-affinity Ca2+ sites, one low-affinity Mg2+ site, and a voltage sensor. This study further investigates the mechanism of Mg2+ activation by using single-channel recording to determine separately the action of Mg2+ on the open and closed states of the channel. To limit Mg2+ action to the Mg2+ sites, the two high-affinity Ca2+ sites are disabled by mutation. When the voltage is stepped from negative holding potentials to +100 mV, we find

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29

Zaree, Pouya, Ilhan Tomris, Sander D. de Vos, et al. "Facile electrochemical affinity measurements of small and large molecules." RSC Advances 13, no. 14 (2023): 9756–60. http://dx.doi.org/10.1039/d3ra01029e.

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A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins and used for determining their binding affinities for small molecules and proteins.

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Di Bartolo, Ary Lautaro, and Diego Masone. "Synaptotagmin-1 C2B domains cooperatively stabilize the fusion stalk via a master-servant mechanism." Chemical Science 13, no. 12 (2022): 3437–46. http://dx.doi.org/10.1039/d1sc06711g.

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31

Qin, Yanru, Jingfan Xie, Shuting Li, et al. "A boronate affinity MIP-based resonance light scattering sensor for sensitive detection of glycoproteins." Analytical Methods 10, no. 42 (2018): 5112–17. http://dx.doi.org/10.1039/c8ay01053f.

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32

Liu, Chin-Wei, Chi-Chang Lin, Li-Chia Chen, Shih-Kang Fan, and Hsien-Chang Chang. "AN AFFINITY SENSOR IMPROVED BY EWOD ACTUATOR-BASED MICROFLUIDIC CHIP." Biomedical Engineering: Applications, Basis and Communications 21, no. 06 (2009): 461–65. http://dx.doi.org/10.4015/s1016237209001659.

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We propose an improved immunoassay method with EWOD-based microfluidic biochip. Electro-wetting on dielectric (EWOD) is based on contact angle-changed phenomenon of droplet on the channel surface. The reactant-containing droplet can be moved from reservoir to a modified electrode area, which a stir function can be provided by controlling AC electro-osmosis flow (ACEOF). The immunochemistry reaction takes place on our modified area via electro-wetting actuation, and the signal from fluorescent or electrochemical impedance spectroscopy (EIS) was used to confirm the performance of designed elemen

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33

Zhang, Ke, Guang Zhang, Xiuwu Yu, Shaohua Hu, and Moxiao Li. "Clustering the sensor networks based on energy-aware affinity propagation." Computer Networks 207 (April 2022): 108853. http://dx.doi.org/10.1016/j.comnet.2022.108853.

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34

Schultz, Jerome S. "Thirty-Fifth Anniversary of the Optical Affinity Sensor for Glucose." Journal of Diabetes Science and Technology 9, no. 1 (2014): 153–55. http://dx.doi.org/10.1177/1932296814552477.

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35

Minunni, Maria, Petr Skládal, and Marco Mascini. "A Piezoelectric Quartz Crystal Biosensor as a Direct Affinity Sensor." Analytical Letters 27, no. 8 (1994): 1475–87. http://dx.doi.org/10.1080/00032719408006383.

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36

Marvin, Jonathan S., Benjamin Scholl, Daniel E. Wilson, et al. "Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR." Nature Methods 15, no. 11 (2018): 936–39. http://dx.doi.org/10.1038/s41592-018-0171-3.

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37

Caldwell, Stuart T., Andrew G. Cairns, Marnie Olson, et al. "Synthesis of an azido-tagged low affinity ratiometric calcium sensor." Tetrahedron 71, no. 51 (2015): 9571–78. http://dx.doi.org/10.1016/j.tet.2015.10.052.

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38

Betty, C. A., R. Lal, D. K. Sharma, J. V. Yakhmi, and J. P. Mittal. "Macroporous silicon based capacitive affinity sensor—fabrication and electrochemical studies." Sensors and Actuators B: Chemical 97, no. 2-3 (2004): 334–43. http://dx.doi.org/10.1016/j.snb.2003.09.008.

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Xian Huang, Siqi Li, J. Schultz, Qian Wang, and Qiao Lin. "A Capacitive MEMS Viscometric Sensor for Affinity Detection of Glucose." Journal of Microelectromechanical Systems 18, no. 6 (2009): 1246–54. http://dx.doi.org/10.1109/jmems.2009.2034869.

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40

Yan, Xinhao, Jin Ju Kim, Hye Sun Jeong, et al. "Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts." Inorganic Chemistry 56, no. 8 (2017): 4332–46. http://dx.doi.org/10.1021/acs.inorgchem.6b02786.

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41

Diem, Peter, Lucas Kalt, Ulrich Haueter, et al. "Clinical Performance of a Continuous Viscometric Affinity Sensor for Glucose." Diabetes Technology & Therapeutics 6, no. 6 (2004): 790–99. http://dx.doi.org/10.1089/dia.2004.6.790.

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42

Beyer, U., D. Schäfer, A. Thomas, et al. "Recording of subcutaneous glucose dynamics by a viscometric affinity sensor." Diabetologia 44, no. 4 (2001): 416–23. http://dx.doi.org/10.1007/s001250051637.

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43

Lenain, Pieterjan, Sarah De Saeger, Bo Mattiasson, and Martin Hedström. "Affinity sensor based on immobilized molecular imprinted synthetic recognition elements." Biosensors and Bioelectronics 69 (July 2015): 34–39. http://dx.doi.org/10.1016/j.bios.2015.02.016.

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Chen, Shiyu, Xiuxiao Yuan, Wei Yuan, Jiqiang Niu, Feng Xu, and Yong Zhang. "Matching Multi-Sensor Remote Sensing Images via an Affinity Tensor." Remote Sensing 10, no. 7 (2018): 1104. http://dx.doi.org/10.3390/rs10071104.

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Lin, Hai, Zhihong Chen, and June Li. "Affinity propagation-based interference-free clustering for wireless sensor networks." International Journal of Communication Systems 33, no. 5 (2019): e4273. http://dx.doi.org/10.1002/dac.4273.

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Akgönüllü, Semra, Seçkin Kılıç, Cem Esen, and Adil Denizli. "Molecularly Imprinted Polymer-Based Sensors for Protein Detection." Polymers 15, no. 3 (2023): 629. http://dx.doi.org/10.3390/polym15030629.

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The accurate detection of biological substances such as proteins has always been a hot topic in scientific research. Biomimetic sensors seek to imitate sensitive and selective mechanisms of biological systems and integrate these traits into applicable sensing platforms. Molecular imprinting technology has been extensively practiced in many domains, where it can produce various molecular recognition materials with specific recognition capabilities. Molecularly imprinted polymers (MIPs), dubbed plastic antibodies, are artificial receptors with high-affinity binding sites for a particular molecul

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Yang, Shaoming, Chaopeng Bai, Yu Teng, et al. "Study of horseradish peroxidase and hydrogen peroxide bi-analyte sensor with boronate affinity-based molecularly imprinted film." Canadian Journal of Chemistry 97, no. 12 (2019): 833–39. http://dx.doi.org/10.1139/cjc-2019-0134.

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A novel electrochemical horseradish peroxidase (HRP) sensor was developed based on boronate affinity-based electropolymerized polythionine (PTh) molecularly imprinted polymer (MIP) as specific recognition element for HRP on gold nanoparticles (AuNPs) modified glassy carbon electrode, in which PTh acted as the electrochemical probe for the sensor. The sensor was characterized by scanning electron microscopy and electron dispersive spectroscopy. Electrochemical impedance spectroscopy, cyclic voltammetry, and differential pulse voltammetry were exploited for the study of the properties of the MIP

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Cohen-Armon, Malka. "Are Voltage Sensors Really Embedded in Muscarinic Receptors?" International Journal of Molecular Sciences 24, no. 8 (2023): 7538. http://dx.doi.org/10.3390/ijms24087538.

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Unexpectedly, the affinity of the seven-transmembrane muscarinic acetylcholine receptors for their agonists is modulated by membrane depolarization. Recent reports attribute this characteristic to an embedded charge movement in the muscarinic receptor, acting as a voltage sensor. However, this explanation is inconsistent with the results of experiments measuring acetylcholine binding to muscarinic receptors in brain synaptoneurosomes. According to these results, the gating of the voltage-dependent sodium channel (VDSC) acts as the voltage sensor, generating activation of Go-proteins in respons

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Puiu, Mihaela, Lucian-Gabriel Zamfir, Valentin Buiculescu, Angela Baracu, Cristina Mitrea, and Camelia Bala. "Significance Testing and Multivariate Analysis of Datasets from Surface Plasmon Resonance and Surface Acoustic Wave Biosensors: Prediction and Assay Validation for Surface Binding of Large Analytes." Sensors 18, no. 10 (2018): 3541. http://dx.doi.org/10.3390/s18103541.

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Abstract:

In this study, we performed uni- and multivariate data analysis on the extended binding curves of several affinity pairs: immobilized acetylcholinesterase (AChE)/bioconjugates of aflatoxin B1(AFB1) and immobilized anti-AFB1 monoclonal antibody/AFB1-protein carriers. The binding curves were recorded on three mass sensitive cells operating in batch configurations: one commercial surface plasmon resonance (SPR) sensor and two custom-made Love wave surface-acoustic wave (LW-SAW) sensors. We obtained 3D plots depicting the time-evolution of the sensor response as a function of analyte concentration

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Pesavento, Maria, Simone Marchetti, Letizia De Maria, Luigi Zeni, and Nunzio Cennamo. "Sensing by Molecularly Imprinted Polymer: Evaluation of the Binding Properties with Different Techniques." Sensors 19, no. 6 (2019): 1344. http://dx.doi.org/10.3390/s19061344.

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Abstract:

The possibility of investigating the binding properties of the same molecularly imprinted polymer (MIP), most probably heterogeneous, at various concentration levels by different methods such as batch equilibration and sensing, is examined, considering two kinds of sensors, based respectively on electrochemical and surface plasmon resonance (SPR) transduction. As a proof of principle, the considered MIP was obtained by non-covalent molecular imprinting of 2-furaldehyde (2-FAL). It has been found that different concentration ranges of 2-FAL in aqueous matrices can be measured by the two sensing

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