Academic literature on the topic 'Agalactiae'

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Journal articles on the topic "Agalactiae"

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Babazadeh, M., S. A. Pourbakhsh, Z. Noormohammadi, M. Esmaelizad, and H. Goudarzi. "Novel Mycoplasma Agalactiae with new P30 Protein Pattern by Major Change in 17 Amino Acids." Journal of Molecular Biology Research 8, no. 1 (January 1, 2018): 8. http://dx.doi.org/10.5539/jmbr.v8n1p8.

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M. agalactiae (Mycoplasma agalactiae) is the main agent of CA (contagious agalactia) that is primarily disease of dairy sheep and goats. In Iran, a three-valent Agalactia inactivated vaccine were prepared based on three M. agalactiae isolated from milk secretion of sheep and goat of Taleghan, Shiraz and Lorestan and preventive policies against CA disease founded in Razi Institute since 1966. The P30 is a specific and stable lipoprotein of M. agalactiae. In this study, complete coding sequence of P30 gene of three Iranian vaccine strains and ten recently field isolates were analyzed in nucleotide and protein levels by bioinformatics tools. Interestingly, results observed a novel protein pattern K106VLKTKEIRLSQERKLS122 in P30 protein of vaccine strains compared to field isolates and the other available sequences in GenBank. Our findings demonstrated different B and T cell epitope patterns in Iranian vaccine strains. We suggested that the major change in P30 protein pattern may be occurred due to mutation during adaptation process in PPLO (Pleuropneumonia-Like Organisms) broth media.
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Yu-Ping, Jia, Zhou Dong-Shun, Zhao Hong-Kun, Wan Ren-Zhong, Liu Wen-Qiang, and Hu Jing-Dong. "A nested PCR-based method for detection ofStreptococcus agalactiae16S rRNA in milk and its application." Chinese Journal of Agricultural Biotechnology 3, no. 2 (August 2006): 115–18. http://dx.doi.org/10.1079/cjb200694.

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AbstractBovine mastitis caused byStreptococcus agalactiaeis mainly subclinical and therefore can be diagnosed only in the laboratory. A nested polymerase chain reaction (PCR)-based method for specific, sensitive and rapid detection ofS. agalactiaein raw milk was developed. The general streptococci primers, which anneal to conserved areas within the 16S rRNA subunit gene, were used as positive controls. The specificity ofS. agalactiaeprimers is based on various areas within conserved areas of the 16S rRNA genes ofS. agalactiae. Results have indicated that the method enables the detection of 1 CFU/ml ofS. agalactiaein raw milk after enrichment, followed by DNA extraction using a rapid and simple procedure developed for this purpose, and specific PCR reaction. The method developed can be used efficiently in the early infectious status investigation ofS. agalactiaein the dairy herd and in prevention and control ofS. agalactiaespread in a herd.
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Khezri, M., and SA Pourbakhsh. "A Survey of Mycoplasma Agalactiae in Small Ruminants with Contagious Agalactiae Syndrome in Iran." Bangladesh Journal of Veterinary Medicine 12, no. 1 (September 16, 2014): 67–72. http://dx.doi.org/10.3329/bjvm.v12i1.20466.

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Contagious agalactia (CA) is one of the major animal health problems in small ruminants. It has economic effect and is caused by Mycoplasma agalactiae, the ‘classic’ etiological agent in sheep and goats. The significance of the different Mycoplasma spp causing CA varies depending on the geographic area. This study conducted between 2012 and 2013 on 189 small ruminants with CA signs in the west of Iran, an area where CA is endemic. All samples (milk, synovial fluid, ear swabs, conjunctival swabs and nasal swabs) were examined by PCR method. Mycoplasma spp was detected in 76.2% and M. agalactiae isolated from 16% of positive samples. M. agalactiae were isolated from 7 conjunctival swabs, 15 milk samples and one synovial fluid sample. Results showed that M. agalactiae was found to be the non-main cause of CA in small ruminants in Iran.DOI: http://dx.doi.org/10.3329/bjvm.v12i1.20466 Bangl. J. Vet. Med. (2014). 12 (1): 67-72
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Migliore, Sergio, Roberto Puleio, Robin A. J. Nicholas, and Guido R. Loria. "Mycoplasma agalactiae: The Sole Cause of Classical Contagious Agalactia?" Animals 11, no. 6 (June 15, 2021): 1782. http://dx.doi.org/10.3390/ani11061782.

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Contagious agalactia (CA) is suspected when small ruminants show all or several of the following clinical signs: mastitis, arthritis, keratoconjunctivitis and occasionally abortion. It is confirmed following mycoplasma isolation or detection. The historical and major cause is Mycoplasma agalactiae which was first isolated from sheep in 1923. Over the last thirty years, three other mycoplasmas (Mycoplasma mycoides subsp. capri, Mycoplasma capricolum subsp. capricolum and Mycoplasma putrefaciens) have been added to the etiology of CA because they can occasionally cause clinically similar outcomes though nearly always in goats. However, only M. agalactiae is subject to animal disease regulations nationally and internationally. Consequently, it makes little sense to list mycoplasmas other than M. agalactiae as causes of the OIE-listed CA when they are not officially reported by the veterinary authorities and unlikely to be so in the future. Indeed, encouraging countries just to report M. agalactiae may bring about a better understanding of the importance of CA. In conclusion, we recommend that CA should only be diagnosed and confirmed when M. agalactiae is detected either by isolation or molecular methods, and that the other three mycoplasmas be removed from the OIE Manual of Diagnostic Tests and Vaccines in Terrestrial Animals and associated sources.
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Kumar, Amit, Anu Rahal, Sandip Chakraborty, Amit Kumar Verma, and Kuldeep Dhama. "Mycoplasma agalactiae, an Etiological Agent of Contagious Agalactia in Small Ruminants: A Review." Veterinary Medicine International 2014 (2014): 1–13. http://dx.doi.org/10.1155/2014/286752.

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Mycoplasma agalactiaeis one of the causal agents of classical contagious agalactia (CA), a serious, economically important but neglected enzootic disease of small ruminants. It occurs in many parts of the world and most notably in the Mediterranean Basin. Following the infection common complications are septicaemia, mastitis, arthritis, pleurisy, pneumonia, and keratoconjunctivitis. Primary or tentative diagnosis of the organism is based upon clinical signs. Various serological tests, namely, growth precipitation, immunofluorescence, complement fixation test, haemagglutination inhibition, agglutination, immunodiffusion, enzyme immunoassays, immunoelectrophoresis, blotting techniques, and others, are available. Molecular tools seem to be much more sensitive, specific, and faster and help to differentiate various strains. The real-time PCR, multiplex PCR, quantitative PCR, PCR-RFLP, MLST, and gene probes, complementary to segments of chromosomal DNA or 16S ribosomal RNA (rRNA), have strengthened the diagnosis ofM. agalactiae. Both live attenuated and adjuvant (alum precipitated or saponified) inactivated vaccines are available with greater use of inactivated ones due to lack of side effects. The present review discusses the etiology, epidemiology, pathogenesis, and clinical signs of contagious agalactia in small ruminants along with trends and advances in its diagnosis, treatment, vaccination, prevention, and control strategies that will help in countering this disease.
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Hardi, Esti Handayani, Sukenda Sukenda, Enang Harris, and Angela Mariana Lusiastuti. "Toksisitas Produk Ekstrasellular (ECP) Streptococcus agalactiae pada Ikan Nila (Oreochromis niloticus)." Jurnal Natur Indonesia 13, no. 3 (October 21, 2012): 187. http://dx.doi.org/10.31258/jnat.13.3.187-199.

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This research aimed to know the toxicity of extracellular products (ECP) of Streptococcus agalactiae was tastedin cultured Nile tilapia (Oreochromis niloticus). Streptococcus agalactiae had two haemolytic types: β-haemolyticand non-haemolytic type. Toxicity test of ECP to know the virulancy factor of S. agalactiae was still limited. It wasfound that after tested on 15 fish weighing 15 g through intraperitoneal injection 0,1 ml/fish, both bacteria causedchanges in swimming pattern, palatability, external and internal anatomy macroscopically and microscopically.Extracellular products of S. agalactiae non-haemolytic type (BHIA and BHI 24 h) and β-haemolytic type (BHI 72 h)caused mortality 12 hours after injection and the mortality continued till day 7 th of culture. Whirling happened 96hours after injection with ECP S. agalactiae β-haemolytic type (BHIA 72 h incubation) whereas injection with ECP(BHI 24 h) on 72 h after injection and continued untill day 7 th. Behavior disease signs caused by S. agalactiaeoccured on eyes. There were opacity, purulens, eye shrink, lateral and bilateral exopthalmia and haemorrhage oninfected-fish. Silver staining of sodium dodecyl sulphate-polyacrylamide gels to S. agalactiae revealed thatpredominant 51.8-69.6 kDa bands were present in BHIA ECP fraction. The 69.6 kDa was absent from the BHI ECP.Total protein on non-haemolytic S. agalactiae ECP are 28.18 ppm on BHIA medium and 13.64 ppm on BHI medium.Whereas β-haemolytic S. agalactiae ECP are 2.73 ppm on BHIA medium and 8.18 ppm on BHI medium. Concentrationof protein in ECP was one of factor that caused non-haemolytic S. agalactiae more virulent than β-haemolytic type.The conclusion from the research that ECP was virulent factor on β-haemolytic and non-haemolytic S. agalactiaein fish which caused changes in behavior disease signs.
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Matos, Rodrigo A. T., Sandra B. Santos, Renato V. Alves, Ednaldo J. Silva, Melânia L. Marinho, José Wilton P. Júnior, Rinaldo A. Mota, and Felicio Garino Júnior. "Ocurrence and risk factors associated with Mycoplasma agalactiae infection in dairy goat herds of Paraíba State, Brazil." Pesquisa Veterinária Brasileira 39, no. 2 (February 2019): 93–98. http://dx.doi.org/10.1590/1678-5150-pvb-5538.

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ABSTRACT: Mycoplasmosis is a disease that may cause severe economical losses in goat and sheep herds, and it is associated with mastitis, polyarthritis, agalactia, conjunctivitis, pneumonia and reproductive failure. The objective of this study was to determine the occurrence of Mycoplasma agalactiae in milk samples and investigate the main risk factors associated with infection in goats from farms of the state of Paraíba, Brazil. For Mycoplasma agalactiae diagnosis, 251 milk samples were submitted to DNA extraction using a commercially available kit, following the manufacturer’s instructions and Polymerase Chain Reaction (PCR) was performed. In addition, questionnaires were applied to identify the main risk factors associated with contagious agalactia. Out of the two hundred fifty-one samples analyzed, 50 (19.9%, I.C. 15.1-25.4%) were PCR positive for M. agalactiae. In the risk factors analysis, some associations were observed for the following variables: size of the herd (P<0.001, OR=7.1, I.C. 2.4-20.6), replacement of farm animals (P<0.001, OR=4.7, I.C. 1.8-12.2) and participation of animals in fairs and exhibitions (P=0.029, OR=2.0, I.C.1.0-3.9). The results allowed confirming the occurrence of Mycoplasma agalactiae in milk samples of goats from Paraíba. Therefore, it is strictly necessary to monitor dairy goat flocks and to raise the awareness of farmers about the economic importance of the disease, since it causes severe economic losses for producers of the state. Identification of risk factors is essential for adoption of control measures and for the correction of the management factors in farms where there are animals with positive diagnosis, avoiding, so, pathogen dissemination.
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Zendulková, D., A. Madanat, P. Lány, K. Rosenbergová, and Z. Pospíšil. "Detection of Mycoplasma agalactiae by Polymerase Chain Reaction in Jordanian Sheep and Goat Herds." Acta Veterinaria Brno 76, no. 1 (2007): 71–77. http://dx.doi.org/10.2754/avb200776010071.

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The aim of the study was to ascertain whether sheep and goats from selected Jordanian herds were infected with Mycoplasma agalactiae, the most common aetiological agent of contagious agalactia of sheep and goats. All examined animals showed clinical signs of disease at the time of sample collection. The group included 35 animals, 15 sheep and 20 goats. For microbiological examination, a total of 107 swabs were taken from conjunctival, nasal, vaginal or preputial mucosae and from the external auditory canal. Identification of the species isolated was carried out by a polymerase chain reaction. Of the 35 animals, 21 (4 sheep and 17 goats) tested positive for Mycoplasma agalactiae. These results confirmed our assumption that this mycoplasma species is present in Jordanian herds and, for the first time, provided evidence that contagious agalactia of sheep and goats occurs in Jordan.
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Fusco, Marisa, Lucia Corona, Toniangelo Onni, Elisabetta Marras, Carla Longheu, Graziano Idini, and Sebastiana Tola. "Development of a Sensitive and Specific Enzyme-Linked Immunosorbent Assay Based on Recombinant Antigens for Rapid Detection of Antibodies against Mycoplasma agalactiae in Sheep." Clinical and Vaccine Immunology 14, no. 4 (February 7, 2007): 420–25. http://dx.doi.org/10.1128/cvi.00439-06.

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ABSTRACT We developed a new recombinant enzyme-linked immunosorbent assay (rELISA) for serodiagnosis of contagious agalactia (CA), a disease caused by Mycoplasma agalactiae in sheep and goats. The assay is based on two M. agalactiae surface proteins, namely, P80 and P55. Identification of these immunodominant and common antigens was accomplished by examining the antibody response elicited in sheep during experimental infection and comparing it to the protein expression profiles of 75 M. agalactiae field strains. Our rELISA was tested with 343 sera, collected from sheep with a laboratory-confirmed diagnosis of CA (n = 223) and from healthy animals (n = 120). All sera had previously been tested by Western blotting (WB) for reactivity against M. agalactiae. In addition, our rELISA was compared with a commercial routine ELISA based on inactivated antigens (CHEKiT). Among the 223 samples that were WB positive for M. agalactiae, 209 (93.7%) tested positive for rP80-P55 with our ELISA, whereas only 164 (73.8%) tested positive with the CHEKiT ELISA. Among the 120 samples tested that were WB negative for M. agalactiae, 96.7% were confirmed as negative with our rELISA, while only 75.8% were confirmed as negative with the CHEKiT ELISA. A comparison of the results with receiver operating characteristic curves indicated that the differences observed between our rELISA and the CHEKiT ELISA are statistically significant. The use of recombinant peptides instead of inactivated antigens could significantly improve the discrimination of positive and negative animals, bringing significant advantages in controlling the import/export of live animals and helping in eradication of this economically detrimental disease.
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Febrianti, Rita, Taukhid Taukhid, and Angela Mariana Lusiastuti. "KERENTANAN IKAN NILA SULTANA, RED NIFI, SRIKANDI DAN AUREUS TERHADAP INFEKSI BAKTERI Streptococcus agalactiae." Jurnal Riset Akuakultur 10, no. 2 (June 30, 2015): 221. http://dx.doi.org/10.15578/jra.10.2.2015.221-230.

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Penelitian ini bertujuan untuk menguji kerentanan empat strain ikan nila, yaitu: Sultana, Red NIFI, Srikandi, dan Aureus terhadap infeksi bakteri Streptococcus agalactiae. Ikan uji berukuran 15-20 g/ekor dan berasal dari populasi, serta batch umur yang sama. Infeksi bakteri S. agalactiae dilakukan secara buatan melalui penyuntikan intra peritoneal (IP) pada dosis 104 cfu/mL, sedangkan kelompok kontrol diinjeksi dengan larutan Phosphate Buffered Saline (PBS). Pengamatan dilakukan terhadap gejala klinis dan mortalitas ikan uji yang berlangsung selama 14 hari. Hasil penelitian menunjukkan bahwa seluruh strain ikan nila mengalami respons yang sama terhadap infeksi bakteri S. agalactie yang ditandai dengan munculnya gejala klinis seperti: warna gelap/menghitam, sirip geripis, nekrosa pada mulut, mata menonjol, opaque, ulcer, dan dropsy. Kerentanan tertinggi terhadap infeksi bakteri S. agalactiae yang dimanifestasikan dengan rataan persentase mortalitas ikan uji diperoleh pada ikan nila Aureus sebesar 72%, Sultana 50%, Srikandi 36%, Red NIFI sebesar 24%, dan kontrol tidak ada mortalitas. Setelah diuji tantang, kadar limfosit mengalami kenaikan, netrofil dan monosit mengalami penurunan.
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Dissertations / Theses on the topic "Agalactiae"

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Carvalho, Vítor Emanuel Duarte. "Clínica médico-cirurgica e reprodução de espécies pecuárias." Master's thesis, Universidade de Évora, 2015. http://hdl.handle.net/10174/17610.

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O presente relatório de estágio visa descrever as atividades desenvolvidas durante o estágio curricular efetuado no âmbito do Mestrado Integrado em Medicina Veterinária da Universidade de Évora, assim como a apresentação de um caso clínico de agaláxia contagiosa numa exploração e correspondente revisão bibliográfica sobre a doença. A primeira parte consiste na apresentação da casuística observada durante o estágio por áreas de intervenção e uma breve abordagem teórica sobre as doenças mais frequentes ou relevantes. A segunda parte do relatório corresponde à revisão bibliográfica acerca da agaláxia contagiosa em pequenos ruminantes, provocada por bactérias do género Mycoplasma spp, maioritariamente, Mycoplasma agalactiae. A doença está distribuída mundialmente, mas com particular incidência nos países da bacia do mediterrâneo. A terceira parte comporta a descrição de um caso clínico acompanhado durante a realização do estágio, onde uma exploração leiteira de ovinos surge com sinais sugestivos de agaláxia contagiosa, sendo efetuado o diagnóstico, o tratamento e a profilaxia desta doença; Abstract: Medical-surgical clinics and breeding livestock species This report describes the activities developed during the traineeship undertaken within the frame of the Master Degree in Veterinary Medicine of the University of Évora, as well as the presentation of a clinical case of contagious agalactia in a farm and the corresponding bibliographic review of the disease. The first part consists in presenting the clinical cases observed during the internship, organizing them by intervention areas, with a brief theoretical review of the most common or relevant pathologies. The second part of the report corresponds to the literature review of contagious agalactia in small ruminants, which is caused by bacteria of the genus Mycoplasma spp, mostly Mycoplasma agalactiae. The disease has a worldwide distribution, but with a particular incidence in the Mediterranean basin countries. The third part includes the description of clinical cases observed while attending the internship, where a dairy sheep farm presented clinical signs suggestive of contagious agalactia, where the diagnosis, treatment and prophylaxis were subsequently made.
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Barbosa, Maysa Santos. "Clonagem, purificação e caracterização de proteínas antigênicas recombinantes obtidas de Mycoplasma agalactiae." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-20022017-144035/.

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A agalaxia contagiosa é uma doença de notificação obrigatória que causa severas perdas econômicas para produção de ovinos e caprinos mundialmente. Apesar de seu impacto na produção animal, pouco se sabe sobre os fatores de virulência e patogenicidade do M. agalactiae (principal agente etiológico). Desta maneira, o presente estudo possuiu como objetivo a identificação, purificação e caracterização de proteínas antigênicas de M. agalactiae. Para tanto, quatro proteínas de superfície com potencial antigênico (WP_011949419.1, WP_011949418.1 (P40), WP_011949336.1, WP_011949770.1) foram selecionadas. Essas proteínas foram expressas em Escherichia coli e purificadas em coluna de níquel. As proteínas purificadas foram avaliadas quanto a antigenicidade em Western blotting utilizando soros de caprinos naturalmente infectados com M. agalactiae. Todas as proteínas expressas foram imunorreativas aos soros de caprinos naturalmente infectados, demonstrando que as proteínas utilizadas nesse estudo são possivelmente antigênicas e possuem epítopos acessíveis.
The Contagious agalactia is a notifiable disease that causes severe economic losses to sheep and goats worldwide. Despite its impact on animal production, little is known about the virulence factors and pathogenicity of M. agalactiae (main etiological agent). Thus, the present study identified, purified and characterized antigenic proteins of M. agalactiae. Therefore, four surface proteins with antigenic potential (WP_011949419.1, WP_011949418.1 (P40), WP_011949336.1, WP_011949770.1) were selected. These proteins were expressed in Escherichia coli and purified on nickel column. The purified proteins were assayed for antigenicity by Western blotting using goat sera naturally infected with M. agalactiae. All expressed proteins were immunoreactive with sera from naturally infected goats, demonstrating that the proteins used in this study are possibly antigenics and it have acessible epitopes.
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Wennekamp, Julia [Verfasser], and Philipp [Akademischer Betreuer] Henneke. "Modulation of phagocyte apoptosis by Streptococcus agalactiae = Modulation von Phagozytenapoptose durch Streptococcus agalactiae." Freiburg : Universität, 2011. http://d-nb.info/1123462895/34.

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Salloum, Mazen. "Les infections à "Streptococus agalactiae" chez l'adulte : emergence et impact de la lysogénie." Thesis, Tours, 2010. http://www.theses.fr/2010TOUR3144/document.

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Streptococcus Agalactiae est depuis les années 1990, responsable d'infections invasives émergentes chez l'adulte. Nous montrons queles souches responsables de ces infections appartiennent majoritairement aux sérotypes V et Ia et aux deux clones phylogénétiquement éloignés, CC1 et CC23. L'étude du contenu prophagique montre une lysogénie fréquente suggérant l'importance de la lysogénie dans la spécialisation de ces souches particulièrement aptes à infecter l'adulte. Dans un deuxième temps, nous avons isolé sept phages tempérés de souches associées à des infections cutanées et ostéo-articulaires. Ces phages appartiennent à la famille des SIPHOVIRIDAE. L’analyse par restriction enzymatique de l’ADN phagique et l’amplification par PCR de fragments d’ADN prophagique a montré la diversité de ces phages et leurdifférence des phages isolés de souches associées aux infections materno-foetales. Les phagesisolés de souches lysogènes de CC1 ont présenté un spectre lytique étendu aux souches de tous les clones intra-species
Streptococcus agalactiae has emerged since 1990 in infections in nonpregnant adults, We showed that the strains isolated from adult infections were mainly of serotypes V and Ia., and mainly belonged to the two phylogenetically distant clones, CC1 and CC23. The prophagic content study showed a frequent lysogeny, suggesting a role of lysegeny in the specialization of these strains able to infect adult. Also, we isolated seven phages from strains associated with cutaneous and osteoarticular infections in adult. Ces phages classified among SIPHOVIRIDAE. Restriction analysis of phagic DNA and PCR for prophagic DNA showed genetiacally diverse phages, distinct from the phages isolated from strains responsible for materno-foetal infections. Phages isolated from lysogenic strains of CC1 had a wide lytic spectrum and were able to lyse strains belonging to all clones intra-species
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Ng, Yi-ting, and 吳依婷. "Multilocus sequence typing for streptococcus agalactiae." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46699569.

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Delannoy, Christian M. J. "Host adaptation of aquatic Streptococcus agalactiae." Thesis, University of Stirling, 2013. http://hdl.handle.net/1893/17259.

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Streptococcus agalactiae is a pathogen of multiple hosts. The bacterium, an aetiological agent of septicaemia and meningo-encephalitis in freshwater and saltwater fish species, is considered a major threat to the aquaculture industry, particularly for tilapia. Cattle and humans are however the main known reservoirs for S. agalactiae. In humans, the bacterium (commonly referred to as Group B Streptococcus or GBS) is a member of the commensal microflora of the intestinal and genito-urinary tracts, but it is also a major cause of neonatal invasive disease and an emerging pathogen in adults. In cattle, S. agalactiae is a well-recognized causative agent of mastitis. Numerous studies focusing on S. agalactiae from human and bovine origins have provided insight into the population structure of the bacterium, as well as the genome content and pathogenic mechanisms through identification of virulence determinants. Concerning S. agalactiae from aquatic origins, scientific information mainly focused on case reporting and/or experimental challenges, with a limited or absence of information in terms of pathogenesis, virulence determinants and genotypes of the strains involved. The objective of this study was to enhance our understanding of the molecular epidemiology, host-adaptation and pathogenicity of S. agalactiae in aquatic species, with particular emphasis on tilapia. Firstly, a collection of 33 piscine, amphibian and sea mammal isolates originating from several countries and continents was assembled, with the aim of exploring the population structure and potential host specificity of aquatic S. agalactiae. Isolates were characterised using pulsed-field gel electrophoresis (PFGE), multi-locus sequence typing (MLST), and a standardised 3-set genotyping system comprising molecular serotypes, surface protein gene profiles and mobile genetic element profiles. Two major subpopulations were identified in fish. The first subpopulation consisted of non-haemolytic isolates that belonged to sequence type (ST) 260 or 261, which are STs that have been reported only from teleosts. These isolates exhibited a low level of genetic diversity by PFGE and clustered with other STs that have been reported only in fish. Another common feature was the absence of all surface protein genes or mobile genetic elements targeted as part of the 3-set genotyping and that are usually found in human or bovine isolates. The second subpopulation consisted of β-haemolytic isolates recovered from fish, frogs and sea mammals, and that exhibited medium to high genetic diversity by PFGE. STs identified among these isolates have previously been identified from strains associated with asymptomatic carriage and invasive disease in humans. The human pathogenic strain ST7 serotype Ia was detected in fish from Asia. Moreover, ST283 serotype III-4 and its novel single locus variant ST491 detected in fish from Southeast Asia shared a 3-set genotype identical to that of an emerging ST283 clone associated with invasive disease of adult humans in Asia. These observations suggested that some strains of aquatic S. agalactiae may present a zoonotic or anthroponotic hazard. STs found among the seal isolates (ST23) have also been reported from humans and numerous other host species, but never from teleosts. This work provided an excellent basis for exploration of the virulence of selected strains in experimental challenges. The virulence of two strains of S. agalactiae was experimentally investigated by intra-peritoneal infection of Nile tilapia (Oreochromis niloticus), using an isolate originally recovered from fish and belonging to ST260, and an isolate originating from a grey seal and belonging to ST23. The clinical signs, the in vivo distribution of viable bacteria and bacterial antigens, and the gross and histopathological lesions that developed during the time course of the infection were investigated. The ST260 strain was highly virulent, whereas no major clinical sign or mortalities occurred in the fish challenged with the ST23 strain. After injection, both strains however gained access to the bloodstream and viable bacteria were recovered from all organs under investigation. During the early stages of infection, bacteria were mostly found within the reticulo-endothelial system of the spleen and kidney. Thereafter, the ST260 demonstrated a particular tropism for the brain and the heart, but granulomatous inflammation and associated necrotic lesions were observed in all organs. ST23 was responsible for a mixed inflammatory response associated with the presence of bacteria in the choroid rete and in the pancreatic tissue only. After 7 days post-challenge and for both strain, the formation or containment of bacteria within granulomata or other encapsulated structures appeared to be a major component of the fish response. However, the load of viable bacteria remained high within organs of fish infected with ST260, suggesting that, unlike ST23, this strain is able to survive within macrophages and/or to evade the immune system of the fish. This work demonstrates that the lack of report of ST23 strains in fish is possibly not due to a lack of exposure but to a lack of virulence in this host. The two strains, which differ in prevalence and virulence in fish, provide an excellent basis to investigate genomic differences underlying the host-association of distinct S. agalactiae subpopulations. The genome of the ST260 strain used in challenge studies was sequenced. We therefore provided the first description for the genome sequence of a non-haemolytic S. agalactiae isolated from tilapia (strain STIR-CD-17) and that belongs by multi-locus sequence typing (MLST) to clonal complex (CC) 552, which corresponds to a presumptive fish-adapted subgroup of S. agalactiae. The genome was compared to 13 S. agalactiae genomes of human (n=7), bovine (n=2), fish (n=3) and unknown (n=1) origins. Phylogenetic analysis based on the core genome identified isolates of CC552 as the most diverged of all S. agalactiae studied. Conversely, genomes from β-haemolytic isolates of CC7 recovered from fish were found to cluster with human isolates of CC7, further supporting the possibility that some strains may represent a zoonotic or anthroponotic hazard. Comparative analysis of the accessory genome enabled the identification of a cluster of genes uniquely shared between CC7 and CC552, which encode proteins that may provide enhanced fitness in specific niches. Other genes identified were specific to STIR-CD-17 or to CC552 based on genomic comparisons; however the extension of this analysis through the PCR screening of a larger population of S. agalactiae suggested that some of these genes may occasionally be present in isolates belonging to CC7. Some of these genes, occurring in clusters, exhibited typical signatures of mobile genetic elements, suggesting their acquisition through horizontal gene transfer. It is not possible to date to determine whether these genes were acquired through intraspecies transfer or through interspecies transfer from the aquatic environment. Finally, general features of STIR-CD-17 highlighted a distinctive genome characterised by an absence of well conserved insertion sequences, an abundance of pseudogenes, a smaller genomic size than normally observed among human or bovine S. agalactiae, and an apparent loss of metabolic functions considered conserved within the bacterial species, indicating that the fish-adapted subgroup of isolates (CC552) has undergone niche restriction. Finally, genes encoding recognised virulence factors in human S. agalactiae were selected and their presence and structural conservation was evaluated within the genome of STIR-CD-17.
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Khosa, Sakshi [Verfasser]. "Nisin resistance in Streptococcus agalactiae / Sakshi Khosa." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2016. http://d-nb.info/1081767618/34.

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Borralheiro, Ricardo Miguel Antunes. "Streptococcus Agalactiae como Agente de Mastites Contagiosas." Dissertação, Instituto de Ciências Biomédicas Abel Salazar, 2008. http://hdl.handle.net/10216/19374.

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Zorgani, Mohamed Amine. "Caractérisation des ARN régulateurs chez Streptococcus agalactiae." Thesis, Tours, 2016. http://www.theses.fr/2016TOUR3309.

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Streptococcus agalactiae, appelé aussi Group B Streptococcus (GBS), est une bactérie commensale du tractus digestif et génital de diverses espèces animales dont l’espèce humaine. Elle représente la première cause d’infections néonatales et est aussi un pathogène émergent chez l’adulte immunodéprimé. L’objectif de ma thèse est la caractérisation fonctionnelle et mécanistique des ARNrég. J’ai étudié plus particulièrement l’ARNrég CetR (pour «cell-envelope-targeting RNA»). Il module la résistance au peptide antimicrobiens (PAM) et la virulence à travers la régulation post-transcriptionnelle de l’ARNm dltD codant une protéine de biosynthèse de l'acide D-alanyl-lipotéichoïque. La délétion de cetR induit des changements dans la morphologie cellulaire, une diminution de la formation du biofilm et de la résistance aux PAM. Une zone d’interaction, CetRdltD, de 27 nucléotides a été prédite in silico. Des mutations compensatoires chez GBS montrent que CetR interagit directement avec l’ARNm dltD et que la perturbation de la zone d’appariement est suffisante pour observer les phénotypes associés à CetR. La quantification des niveaux d’ARNm et de la protéine DltD nous a permis de montrer que CetR active la traduction de dltD et que la perturbation du duplex CetR-dltD induit une diminution spectaculaire de la protéine DltD. De plus, en utilisant un modèle murin d’infection et en quantifiant la survie des bactéries dans les macrophages, nous avons montré que CetR et DltD sont cruciaux pour la virulence de GBS. Enfin, une approche protéomique globale nous a permis de montrer que CetR joue un rôle important dans l’expression des protéines dites « moonlighting » et de certains facteurs de virulence potentiels. Cet ARNrég peut jouer un rôle important dans la capacité de S. agalactiae à s'établir dans son biotope et à exprimer ses facteurs de virulence. Enfin, les résultats de ces recherches sont des prérequis au développement de stratégies permettant de réduire le risque des infections néonatales dues à S. agalactiae
The opportunistic pathogen group B Streptococcus (GBS) is the leading cause of neonatal infections. The aim of this work is the characterization of a 680 nt-long regulatory RNA, CetR (cell-envelope-targeting RNA). It modulates antimicrobial peptides (AMPs) resistance and virulence through posttranscriptional regulation of dltD mRNA which encodes a D-alanyl-lipoteichoic acid biosynthesis protein. Deletion of cetR leads to cell morphology changes, reduced biofilm formation and AMPs resistance. A 27 nt-long CetR-dltD interacting region is predicted in silico. Compensatory base pair exchanges in GBS demonstrate that CetR interacts directly with dltD mRNA and that disruption of this RNA pairing is sufficient to observe the CetR-associated phenotypes. By quantifying both mRNA and protein, we demonstrate that CetR enhances dltD translation and disruption of the CetR/dltD mRNA interaction results in a dramatic decrease in DltD protein. Moreover, using an infection murine model and quantifying bacterial survival in macrophages, we observe that both CetR and DltD are crucial for GBS virulence. Finally, we highlight CetR pleiotropic role in the expression of several moonlighting proteins and potential virulence factors. This regulatory RNA may play an important role in the ability of GBS to settle in its biotope and express its virulence factors
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Torres, Sofia Isabel Ferreira. "Streptococcus agalactiae, avaliação da resistência a macrólidos." Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/10260.

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Mestrado em Microbiologia
Streptococcus ß – hemolítico do grupo B ou S. agalactiae é uma das principais causas de infeções neonatais graves. A maioria das infeções neonatais podem ser prevenidas usando a profilaxia antibiótica intraparto em mulheres colonizadas por esta bactéria e que têm um risco aumentado de transmiti-la aos seus bebés. No entanto, apesar dos ensaios clínicos que demonstram a efetividade da profilaxia antibiótica intraparto, as estratégias de prevenção não foram amplamente e consistentemente implementadas, e a incidência da doença neonatal por este agente não diminui. Para promover uma coordenada aplicação das medidas preventivas, o CDC em conjunto com representantes de associações profissionais, publicou em 1996, recomendações para a prevenção da doença neonatal por Streptococcus agalactiae. Estas recomendações foram revistas em 2002 e mais recentemente em 2010. O objetivo deste estudo foi verificar a ocorrência de colonização por Streptococcus agalactiae em gestantes saudáveis, avaliar a susceptibilidade dos isolados aos antibióticos e determinar os fenótipos de resistência à eritromicina. Realizou-se um estudo envolvendo 212 gestantes que realizaram rastreio para S. agalactiae no Laboratório de Patologia Clínica Hilário de Lima, em Braga, no período de 1 de Julho de 2012 a 31 de Agosto de 2012. Foram colhidas amostras vaginal e retal para rastrear a presença de colonização por S.agalactiae. O procedimento laboratorial para a deteção da colonização por S. agalactiae foi o recomendado pelo CDC. Os rastreios positivos (34) foram avaliados quanto ao perfil de suscetibilidade aos antibióticos de eleição para a profilaxia (penicilina, ampicilina, eritromicina e clindamicina). Os isolados que apresentaram resistência a eritromicina (17.6%) foram estudados para determinar o fenótipo de resistência à eritromicina, sendo que 100% desses isolados apresentaram fenótipo MLSB constitutivo. Neste estudo foram também incluídos 39 isolados de S. agalactiae, obtidos de gestantes que apresentaram rastreio positivo para este agente, sendo este rastreio realizado no Centro Hospitalar do Porto- Hospital de Santo António. Estas amostras foram estudadas quanto ao perfil de resistência à eritromicina e clindamicina apresentando uma taxa de resistência, respetivamente, de 33.4% e 15.4%, sendo que 69.2% dos isolados resistentes à eritromicina apresentavam fenótipo MLSB constitutivo e 30.8% apresentavam o fenótipo M (resistência apenas a macrólidos).
Group B streptococcus is a leading cause of serious neonatal infections. Most neonatal infections can be prevented using the intrapartum antibiotic prophylaxis in women colonized by the bacteria and have an increased risk to transmit it to their babies. However, despite clinical trials that demonstrate the effectiveness of intrapartum antibiotic prophylaxis, prevention strategies were not implemented widely and consistently, and the incidence of neonatal GBS disease has not declined. To promote a coordinated application of preventive measures, the CDC together with representatives of professional associations, published in 1996, recommendations for the prevention of neonatal disease by Streptococcus agalactiae. These recommendations were revised in 2002 and most recently in 2010. The aim of this study was to determine the occurrence of colonization by Streptococcus agalactiae in healthy pregnant women, to evaluate the susceptibility of isolates to antibiotics and determine the phenotypes of erythromycin resistance. We conducted a study involving 212 pregnant women who were screened for S. agalactiae in the Laboratory of Clinical Pathology Hilary of Lima, in Braga, in the period 1 July 2012 to 31 August 2012. Samples were collected vaginal and rectal to track the presence of colonization by S. agalactiae. The testing procedure for detection of colonization by S. agalactiae was recommended by the CDC. The positive screenings (34) were evaluated for susceptibility to antibiotics of choice for prophylaxis (penicillin, ampicillin, erythromycin and clindamycin). The isolates that were resistant to erythromycin (17.6%) were studied to determine the phenotype of resistance to erythromycin, and 100% of these isolates showed constitutive MLSB phenotype. In this study we also included 39 isolates of S. agalactiae obtained from women who had screened positive for this agent, and this screening held at the Centro Hospitalar do Porto – Hospital de Santo António. These samples were studied as to their resistance to erythromycin and clindamycin resistance showing a rate of, respectively, 33.4% and 15.4%, and 69.2% of isolates were resistant to erythromycin constitutive MLSB phenotype and 30.8% had the M phenotype (resistance only macrolides).
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Books on the topic "Agalactiae"

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Verani, Jennifer R. Prevention of perinatal group B streptococcal disease: Revised guidelines from CDC, 2010. Atlanta, GA: Dept. of Health and Human Services, Centers for Disease Control and Prevention, 2010.

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Le Doare, Kirsty, Christine E. Jones, and Paul T. Heath. Group B Streptococcus (Streptococcus agalactiae). Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780190604813.003.0019.

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Group B Streptococcus (GBS) is a leading cause of early neonatal infection and neonatal mortality, with long-term adverse neurodevelopmental outcomes in up to 50% of survivors of GBS meningitis. GBS has a likely underappreciated role in causing preterm birth and stillbirth. GBS colonizes the vagina and gastrointestinal tract of the pregnant woman, and transmission to the infant occurs during or just before delivery. Although the majority of these infants do not develop invasive disease, maternal colonization is a prerequisite for early onset disease (0–6 days of life, most commonly associated with sepsis and respiratory distress) and a significant risk factor for late onset disease (7–89 days of life, most commonly associated with sepsis and meningitis). The introduction of intrapartum antibiotic prophylaxis has resulted in significant declines in the incidence of early onset disease but provides no protection against late onset disease.
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(Foreword), David Heymann, and I. Edward Alcamo (Editor), eds. Streptococcus (Group B) (Deadly Diseases and Epidemics). Chelsea House Publications, 2007.

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Risk profile - Group B Streptococcus (GBS) –​ Streptococcus agalactiae sequence type (ST) 283 in freshwater fish. FAO, 2021. http://dx.doi.org/10.4060/cb5067en.

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Jones, G. E. Contagious Agalactia and Other Mycoplasmal Diseases of Small Ruminants (Agriculture). European Communities, 1987.

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Contagious agalactia and other mycoplasmal diseases of small ruminants: Proceedings of a workshop held in Nice, France, on 19 and 20 September 1985. Luxembourg: Commission of the European Communities, Directorate-General Telecommunications, Information Industries and Innovation, 1987.

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Book chapters on the topic "Agalactiae"

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Nolte, Oliver. "Streptococcus agalactiae." In Lexikon der Infektionskrankheiten des Menschen, 774–79. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-39026-8_1050.

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Raabe, Vanessa N., and Andi L. Shane. "Group B Streptococcus (Streptococcus agalactiae)." In Gram-Positive Pathogens, 228–38. Washington, DC, USA: ASM Press, 2019. http://dx.doi.org/10.1128/9781683670131.ch14.

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Schulz, Eva-Cathrin. "Was wissen Sie zu Streptococcus agalactiae?" In Mikrobiologie für die mündliche Prüfung, 13. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-80349-9_14.

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Young, Hugh, and Marie Ogilvie. "Streptococcus beta-haemolytic group B (Streptococcus agalactiae)." In Genitourinary Infections, 280–87. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-017-5080-6_11.

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Kvam, Augusta I., Lars Bevanger, and Kirsti Løseth. "An Apparently New Strain-Variable Streptococcus agalactiae Protein." In Streptococci and the Host, 355–57. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_85.

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Lars, Bevanger, Odd G. Brakstad, Johan A. Maeland, Augusta I. Kvam, Grethe Iversen, and Randi Valsoe Lyng. "Aberrations in Expression of the β Antigen of Streptococcus agalactiae." In Streptococci and the Host, 999–1001. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_235.

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Johansson, Monika, Amaya Albalat, Aileen Stirling, and William Mullen. "Results of proteomic mapping into milk infected by Streptococus agalactiae." In Farm animal proteomics, 91–93. Wageningen: Wageningen Academic Publishers, 2012. http://dx.doi.org/10.3920/978-90-8686-751-6_21.

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Mullen, William, Amaya Albalat, Aileen Stirling, Justyna Siwy, and Monika Johansson. "Methodology of proteomic mapping into milk infected by Streptococus agalactiae." In Farm animal proteomics, 116–19. Wageningen: Wageningen Academic Publishers, 2012. http://dx.doi.org/10.3920/978-90-8686-751-6_27.

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Rossetti, Roberto, Bruno Maranini, and Piero Soldi. "Vaginal Colonization by Streptococcus agalactiae in Two Neighbouring Populations in Tuscany." In Streptococci and the Host, 249–50. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_60.

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Mat Zin, Ain Auzureen, Ruhil Hayati Hamdan, Mohd Hafiz Jamaludin, Jasni Sabri, and Li Peng Tan. "Histopathology Alteration of Red Hybrid Tilapia (Oreochromis Spp.) Caused by Streptococcus Agalactiae Infection." In Charting the Sustainable Future of ASEAN in Science and Technology, 215–25. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-3434-8_19.

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Conference papers on the topic "Agalactiae"

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Dinu, Cerasela Z., Constantin Grigoriu, Maria Dinescu, Florentina Pascale, Adrian Popovici, Lavinia Gheorghescu, Ana Cismileanu, and Eugenia Avram. "Laser radiation effects on Mycoplasma agalactiae." In International Conference on: Advanced Laser Technologies (ALT'01), edited by Dan C. Dumitras, Maria Dinescu, and Vitali I. Konov. SPIE, 2002. http://dx.doi.org/10.1117/12.478657.

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Giummarra, V., M. C. Scuderi, G. Tempera, L. S. Roccasalva, and P. M. Furneri. "Relationships between hydrophobicity and biofilm formation in Streptococcus agalactiae strains." In Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009). WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814322119_0043.

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"Pathologicl Findings of Experimental Streptococcus Agalactiae Infection in Red Hyprid Tilapia (Oreochromis sp.)." In International Conference on Chemical, Agricultural and Medical Sciences. International Institute of Chemical, Biological & Environmental Engineering, 2013. http://dx.doi.org/10.15242/iicbe.c1213075.

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Hooven, Thomas A., Tara M. Randis, and Hervé Tettelin. "The Stringent Response and Arginine Metabolism Play Key Roles in Streptococcus Agalactiae Toxin Expression." In Selection of Abstracts From NCE 2015. American Academy of Pediatrics, 2017. http://dx.doi.org/10.1542/peds.140.1_meetingabstract.79.

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Marques, Aldilane Lays Xavier, Liliane Patricia Gonçalves, Souza Tenorio, Rayane Martins Botelho, Ana Lucia Mendes da Silva, Julie Bergeron, Guillaume Sébire, et al. "BIOPHYSICAL AND MORPHOLOGICAL OUTCOMES FROM UVAOL TREATMENT IN TROPHOBLAST CELLS INFECTED BY STREPTOCOCCUS AGALACTIAE." In Encontro Anual da biofisica 2019. São Paulo: Editora Blucher, 2019. http://dx.doi.org/10.5151/biofisica2019-73.

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Trinugraha, A. C., T. Handayani, D. Priyoatmojo, and B. J. Tuasikal. "Profil Darah dan Penambahan Bobot Badan Kambing Peranakan Ettawah Setelah Pemberian Vaksin Iradiasi Streptococcus agalactiae." In Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Pusat Penelitian dan Pengembangan Peternakan, 2017. http://dx.doi.org/10.14334/pros.semnas.tpv-2017-p.373-378.

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Ilmiya, Istifadatul, Hening Ryan Aryani, Nur Rohmah Prihatanti, Dwi Yuni Nur Hidayati, and Noorhamdani Noorhamdani. "Identification of hemagglutinin protein from Streptococcus agalactiae pili in mice erythrocytes as a vaccine candidate." In INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020). AIP Publishing, 2021. http://dx.doi.org/10.1063/5.0052701.

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Ramos, Nicolle, Beatriz Silva, Maximiano Teixeira, Nicea Silva, Marco Henrique, and Ivano Filippis. "Molecular characterization of Streptococcus agalactiae group B (SGB) isolated from pregnant women in Rio de Janeiro." In International Symposium on Immunobiological. Instituto de Tecnologia em Imunobiológicos, 2021. http://dx.doi.org/10.35259/isi.2021_46635.

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Santos, Joana Darc Silva, and Ana Karla Araújo Montenegro. "ESTREPTOCOCOS B COMO CAUSA DE INFECÇÃO EM MULHERES GRÁVIDAS: UMA REVISÃO BIBLIOGRÁFICA." In I Congresso Nacional de Microbiologia Clínica On-Line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1158.

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Introdução: Os estreptococos do grupo B (EGB) ou Streptococcus agalactiae, são bactérias gram-positivas, catalase-negativas, anaeróbias facultativas, que apresentam forma esférica ou ovóide, agrupando-se em cadeia, sendo um comensal comum do trato genital feminino; estando ainda, relacionada a infecções invasivas em recém-nascidos (como sepse, meningite e pneumonia). A incidência de colonização no trato genital de gestantes, relacionada às complicações da evolução da gravidez, varia de 10% a 30% e a transmissão vertical ocorre em 30 a 70% dos recém-nascidos. Objetivos: o presente estudo objetivou analisar através da literatura específica, as infecções neonatais por EGB, a fim de apresentar as possíveis consequências da colonização e a relevância do diagnóstico para a detecção pré-parto da bactéria, inspirando estratégias para a redução significativa da incidência. Material e métodos: as informações para este estudo foram levantadas a partir de artigos em revistas científicas, publicados em português, espanhol e inglês, num recorte temporal de 2001 a 2021, nas bases de dados PubMed, SciELO e MEDLINE. Resultados: foram encontrados 1.476 artigos utilizando as palavras chave, gravidez e Streptococcus agalactiae. Entretanto, visto a inespecificidade dos artigos, utilizamos os que apresentavam os descritores do título, totalizando para abordagem neste estudo 79 artigos. Após a leitura dos resumos, 23 estudos foram avaliados por completo, obtendo um número final de 9 artigos que demonstraram a importância para o tema proposto no estudo. Embora a abrangência dos exames pré-natais tenha aumentado, observa-se que ainda hoje a cultura do EGB, não é realizada de forma rotineira durante o pré-natal, o que explica a alta prevalência de colonização materna e, consequentemente, de doenças neonatais. Conclusão: diante da escassez de informações específicas sobre a doença, fica evidente a necessidade de novos estudos no Brasil a fim de subsidiar o desenvolvimento de políticas públicas de saúde, no intuito de fortalecer a atenção dada pelos órgãos responsáveis ao rastreamento do patógeno e a profilaxia durante o acompanhamento pré-natal.
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Pinto, Vanessa Arantes, Gabriela Santos Alencar, Adriano Favero, Hugo Franciscon, and Fagner Luiz Da Costa Freitas. "POTENCIAL MICROBIOLÓGICO DO SYZYGIUM AROMATICUM (L). SOBRE MICRORGANISMOS DE IMPORTÂNCIA NA MEDICINA VETERINÁRIA." In I Congresso On-line Nacional de Clínica Veterinária de Pequenos Animais. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1920.

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Introdução: O fenômeno de resistência a antimicrobianos está se mostrando um grande problema na saúde, ameaçando tanto a população humana como animal. Devido a isso, a descoberta de novos compostos com capacidade antimicrobiana torna-se imprescindível, e os fitoterápicos têm se mostrado uma alternativa promissora na terapia complementar. O óleo essencial (OE) de Syzygium aromaticum (Cravo da Índia), apresenta potencial capacidade antibacteriana e antifúngica devido às altas concentrações de Eugenol. Objetivos: Avaliar o potencial antimicrobiano do óleo essencial de Syzygium aromaticum (L). contra microrganismos de importância na Medicina veterinária. Materiais e métodos: Foram testadas as bactérias gram positivas: S. aureus LB 25923, S. aureus B24, S. aureus NP 38, Streptococcus uberis, S. agalactiae, Enterococcus faecalis e S. epidermidis; as gram negativas: Pseudomonas aeruginosa ATCC 27853 e E. coli ATCC 25922; e os fungos: C. tropicalis e C. gattii 179. Utilizou-se a técnica de disco difusão (Kirby-bauer) aplicando 10µL de OE, e posteriormente, para os patógenos que apresentaram o halo inibitório, determinou-se, aplicando 15µL de OE, a concentração inibitória mínima (CIM), concentração bactericida mínima (CBM) e concentração fungicida mínima (CFM) através da microdiluição seriada utilizando leitor de ELISA e corante resazurina. Resultados: Todos os microrganismos apresentaram halo inibitório. Com relação aos valores de CIM e CBM para as bactérias, obteve-se, respectivamente: S. aureus LB25923 (18,75% e 18,75%), S. aureus B 24 (4,94% e 4,94%) S. aureus NP 38 (3,64% e 3,64%) Streptococcus uberis (2,60% e 3,125%) S. agalactiae (5,20 %, 5,20%), Enterococcus faecalis (5,20% e 6,25%), S. epidermidis (3,38% e 4,42%), Pseudomonas aeruginosa ATCC 27853 (2,09% e 2,09%), E. coli ATCC 25922 (10,41% e 10,41%). Os resultados de CIM e CFM obtidos para os fungos foram respectivamente: C. tropicalis (1,82% e 1,82%) e C. gattii 179 (5,27% e 5,27%). Conclusão: O óleo essencial de Syzygium aromaticum possui considerável potencial antimicrobiano “in vitro”, e dessa forma, pode ser considerado uma possível alternativa para as terapias sintéticas de tratamento das doenças infecciosas bacterianas e fúngicas encontradas na rotina clínica.
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Reports on the topic "Agalactiae"

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Pertuz-Mesa, Yolima, Ingrid Johana Palmera-Castelblanco, Cindy Yamilitza Pimienta-Mejia, Andrea Stefanys Rodríguez-Bolívar, and María Alejandra Rodríguez-Martínez. Infección por Streptococcus agalactiae en mujeres embarazadas entre las semanas 35 y 37 de gestación. Una revisión. Universidad Cooperativa de Colombia, 2017. http://dx.doi.org/10.16925/greylit.2014.

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