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Journal articles on the topic 'Agar diffusion method'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Ahuja, Chitrangana, Harpreet Kaur, and Rajeev Sharma. "Antibacterial Activity of Terminalia Chebula Fruit by Agar well Diffusion Method." Journal of Chemistry, Environmental Sciences and its Applications 1, no. 2 (2015): 67–72. http://dx.doi.org/10.15415/jce.2015.12006.

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Nurhayati, Lilih Siti, Nadhira Yahdiyani, and Akhmad Hidayatulloh. "PERBANDINGAN PENGUJIAN AKTIVITAS ANTIBAKTERI STARTER YOGURT DENGAN METODE DIFUSI SUMURAN DAN METODE DIFUSI CAKRAM." Jurnal Teknologi Hasil Peternakan 1, no. 2 (2020): 41. http://dx.doi.org/10.24198/jthp.v1i2.27537.

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Antibacterial activity testing can be done using the agar diffusion method, including agar well difussion and disk diffusion agar methods. This study aims to compare two antibacterial testing methods to analyze the anti-bacterial activity of a yogurt starter against the bacteria Eschericia coli and Staphilococcus aureus. The study was conducted experimentally with 5 concentrations of yogurt starter, namely 2%, 4%, 6%, 8%, and 10%. Testing antibacterial activity using two methods ,disk diffusion agar and well difussion agar methods. The research showed that agar well diffusion method obtained a

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3

Mariya, George, Pavithra P, N. Sangeetha V, S. Murugan S, N. Sathya T, and S. Kumaravel T. "Comparative Cytotoxicity Assessment of Cobalt-Chromium-Molybdenum Casts Using Elution, Agar Diffusion, and Filter Diffusion Methods." Indian Journal of Science and Technology 18, no. 12 (2025): 993–99. https://doi.org/10.17485/IJST/v18i12.503.

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Abstract <strong>Background/Objectives:</strong> Cobalt-Chromium-Molybdenum (CCM) alloys are widely used in dental abutments due to their mechanical strength, corrosion resistance, and durability in the harsh oral environment. However, despite these advantages, concerns remain regarding their potential cytotoxic effects. Therefore, we investigated the cytotoxic potential of CCM using three different cytotoxicity tests. <strong>Methods:</strong> In the current study, three different methods – Elution Method, Agar Diffusion Method, and Filter Diffusion Method - were used to

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Bazarov, Adham Khayrullayevich, and Ozodbek Olimjon Ogli Sobirov. "Diagnosing Disease Of Cow Mastitis And Method Of Using Diffusion-Precipitation Reaction In Agar Gel." American Journal of Veterinary Sciences and Wildlife Discovery 03, no. 02 (2021): 5–8. http://dx.doi.org/10.37547/tajvswd/volume03issue01-02.

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The microbial factor is essential in the etiology of mastitis. In this regard, bacteriological diagnostics is one of the decisive moments in the recognition and differentiation of pathological conditions of the mammary gland. However, it takes a lot of time to make a diagnosis using general methods, special culture media and reagents.

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TSAI, CHIN-EN, and FUSAO KONDO. "Improved Agar Diffusion Method for Detecting Residual Antimicrobial Agents." Journal of Food Protection 64, no. 3 (2001): 361–66. http://dx.doi.org/10.4315/0362-028x-64.3.361.

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The improved agar diffusion method for determination of residual antimicrobial agents was investigated, and the sensitivities of various combinations of test organisms and assay media were determined using 7 organisms, 5 media, and 31 antimicrobial agents. Bacillus stearothermophilus and synthetic assay medium (SAM) showed the greatest sensitivity for screening penicillins (penicillin G and ampicillin). The combination of Bacillus subtilis and minimum medium (MM) was the most sensitive for tetracyclines (oxytetracycline and chlortetracycline), B. stearothermophilus and SAM or Micrococcus luteu

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Solano, Ana Gabriela Reis, Larissa de Melo Campos Sousa Pereira, Míriam de Fátima Vianna Leonel, and Elzíria de Aguiar Nunan. "Development of agar diffusion method for dosage of gramicidin." Brazilian Journal of Pharmaceutical Sciences 47, no. 3 (2011): 564–72. http://dx.doi.org/10.1590/s1984-82502011000300014.

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Gramicidin, an antimicrobial peptide active against Gram positive bacteria, is commonly used in pharmaceutical preparations for topical use. Considering that only the turbidimetric method has been described in the literature, the present study sought to develop and validate an agar diffusion method for the dosage of gramicidin. The method was developed and validated using the Kocuria rhizophila ATCC 9341 as a test microorganism. Two designs were used: a 3x3 parallel-line model, and a 5x1 standard curve. The validation demonstrated that the method follows the linear model (r²= 0.994), presentin

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7

Zhou, Ying, Meijuan Zhang, and Hui Liu. "Total Antioxidant Capacity of Serum Determined Using the Potassium Permanganate Agar Method Based on Serum Diffusion in Agar." Bioinorganic Chemistry and Applications 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/406071.

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Objectives. To develop a new method for determining total antioxidants in serum and to evaluate the total antioxidant capacity of organisms.Design and Methods. Sodium hyposulfite (Na2S2O3) and serum were used to evaluate the linearity and precision of the potassium permanganate agar method. The area of serum diffusion in samples from 30 intensive care unit (ICU) patients compared with 44 healthy subjects was determined by the potassium permanganate agar method.Results. The linearity (R2in the linear experiment of Na2S2O3was 0.994;R2in the linear experiment of serum was 0.987) and precision (co

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Chiu, Chien-Tung, Chung-Hsu Lai, Yi-Han Huang, Chih-Hui Yang, and Jiun-Nong Lin. "Comparative Analysis of Gradient Diffusion and Disk Diffusion with Agar Dilution for Susceptibility Testing of Elizabethkingia anophelis." Antibiotics 10, no. 4 (2021): 450. http://dx.doi.org/10.3390/antibiotics10040450.

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Elizabethkingia anophelis has recently emerged as a cause of life-threatening infections. This study compared the results of antimicrobial susceptibility testing (AST) conducted for E. anophelis through different methods. E. anophelis isolates collected between January 2005 and June 2019 were examined for their susceptibility to 14 antimicrobial agents by using disk diffusion, gradient diffusion (Etest; bioMérieux S.A., Marcy l’Etoile, France), and agar dilution methods. The agar dilution method was the reference assay. According to the agar dilution method, the isolates exhibited the highest

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9

Patil, Nilima R. "PERFORMANCE OF CHROM AGAR MEDIUM AND CONVENTIONAL METHODS FOR DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS." Asian Journal of Pharmaceutical and Clinical Research 9, no. 6 (2016): 136. http://dx.doi.org/10.22159/ajpcr.2016.v9i6.13916.

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Background:- Methicillin resistant Staphylococcus aureus (MRSA) are responsible for hospital and community acquired infections. There are many laboratory methods for detection of MRSA. Chromogenic media have been used for the last few years for the quick detection of MRSA. Objective:- Aim of this study was to compare the performance of conventional methods and chromogenic media for the detection of MRSA in a tertiary care hospital. Material and method: - 200 consecutive isolates of S. aureus confirmed by conventional methods, collected in a tertiary care hospital were used for this study. Cefo

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Amandita, Fitri Yola, Efadeswarni Efadeswarni, Nabillah Aini, and Lania Yuchanitz Fatma. "Resistance Test of Potential Mercury-Reducing Bacteria Using Agar Plate Method." Journal of Experimental Life Sciences 14, no. 2 (2024): 79–86. http://dx.doi.org/10.21776/ub.jels.2024.014.02.05.

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The restoration of mercury-contaminated sites is a global concern, and countless studies have been conducted to formulate the best remediation practice. Mercury pollution in Indonesia has been a national issue. Thus, the government has enforced the reduction and elimination of this toxic and persistent chemical element and its compounds since 2019. As claimed to be the most environmentally friendly and cost-efficient technique, bioremediation using indigenous bacteria offers excellent potential to be implemented in developing countries such as Indonesia. This study is a preliminary part of fou

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Hemalatha E, Bhuvaneshwari G, and Kalyani M. "Comparative analysis of novel suspension testing and agar cup diffusion methods in establishing the susceptibility of Pseudomonas aeruginosa against ethanol and chlorhexidine gluconate." International Journal of Research in Pharmaceutical Sciences 11, SPL2 (2020): 271–75. http://dx.doi.org/10.26452/ijrps.v11ispl2.2693.

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Pseudomonas aeruginosa is one among the leading nosocomial pathogens worldwide. It is therefore necessary to decrease and to prevent a rebound of growth. Comparison of novel suspension testing method and agar cup diffusion method results in determination of the sensitive method to identify effectiveness of disinfectants against microbial activity. This study was carried out to determine the effectiveness among novel suspension testing and agar cup diffusion method to determine disinfectant susceptibility and also to identify the efficacy of ethanol and chlorhexidine gluconate at manufacturer’s

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Boyanova, Lyudmila, Galina Gergova, Rossen Nikolov, et al. "Activity of Bulgarian propolis against 94 Helicobacter pylori strains in vitro by agar-well diffusion, agar dilution and disc diffusion methods." Journal of Medical Microbiology 54, no. 5 (2005): 481–83. http://dx.doi.org/10.1099/jmm.0.45880-0.

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Propolis exhibits antimicrobial, anti-inflammatory and other biological effects. The aim of this study was to evaluate the activity of 30 % ethanolic extract of Bulgarian propolis against 94 Helicobacter pylori strains by three methods. By the agar-well diffusion method, only 13.8 % of the strains exhibited no inhibition by 30 μl propolis extract (containing 9 mg propolis) and all isolates were inhibited to some extent by 90 μl of the extract (27 mg propolis) per well. The mean diameters of growth inhibition by 30, 60 or 90 μl propolis extract or 30 μl 96 % ethanol per well were 16.8, 19.2, 27

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ÖZYÜREK, Taha, and Ebru ÖZSEZER DEMİRYÜREK. "Evaluation of Antimicrobial Efficacy of Different Root Canal Medicaments by Agar Diffusion Method." Turkiye Klinikleri Journal of Dental Sciences 22, no. 2 (2016): 92–96. http://dx.doi.org/10.5336/dentalsci.2015-48173.

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Tarek, A. El-bashiti. "Production of Cephalosporin C from Acremonium chrysogenum, and its Antimicrobial Activity against Some Pathogenic Bacteria." Pharmaceutical and Chemical Journal 4, no. 2 (2017): 19–23. https://doi.org/10.5281/zenodo.13762620.

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The Production of cephalosporin C by <em>Acremonium chrysogenum</em>in flask contain fermentation medium, after that its extraction, purification and estimation was performed by Spectrophotometric Method. The antimicrobial activities of both the crude and purified cephalosporin C were performed against the test organisms by Agar Well Diffusion Method. Agar well diffusion method and the inhibition zones were recorded and evaluated the effectiveness of the antibiotic against gram positive bacteria (<em>Staphylococcus aereus</em> and <em>Bacillus subtilis</em>) and no effectiveness against gram n

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15

Díez-Aguilar, María, María-Isabel Morosini, Rosa del Campo, María García-Castillo, Javier Zamora, and Rafael Cantón. "In VitroActivity of Fosfomycin against a Collection of Clinical Pseudomonas aeruginosa Isolates from 16 Spanish Hospitals: Establishing the Validity of Standard Broth Microdilution as Susceptibility Testing Method." Antimicrobial Agents and Chemotherapy 57, no. 11 (2013): 5701–3. http://dx.doi.org/10.1128/aac.00589-13.

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ABSTRACTThe broth microdilution method for fosfomycin andPseudomonas aeruginosawas assessed and compared with the approved agar dilution method in 206 genetically unrelatedP. aeruginosaclinical isolates. Essential agreement between the two methods was 84%, and categorical agreement was 89.3%. Additionally, Etest and disk diffusion assays were performed. Results validate broth microdilution as a reliable susceptibility testing method for fosfomycin againstP. aeruginosa. Conversely, unacceptable concordance was established between Etest and disk diffusion results with agar dilution results.

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Fujihara, M., S. Nishiyama, and S. Hasegawa. "Effects of agars on determination of potency of polymyxin B sulfate by the agar plate diffusion method." Antimicrobial Agents and Chemotherapy 38, no. 11 (1994): 2665–67. http://dx.doi.org/10.1128/aac.38.11.2665.

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Matar, Madonna J., Luis Ostrosky-Zeichner, Victor L. Paetznick, Jose R. Rodriguez, Enuo Chen, and John H. Rex. "Correlation between E-Test, Disk Diffusion, and Microdilution Methods for Antifungal Susceptibility Testing of Fluconazole and Voriconazole." Antimicrobial Agents and Chemotherapy 47, no. 5 (2003): 1647–51. http://dx.doi.org/10.1128/aac.47.5.1647-1651.2003.

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ABSTRACT The activities of fluconazole and voriconazole against isolates of Candida spp. (n = 400) were tested by the E-test, disk diffusion, and the National Committee for Clinical Laboratory Standards (NCCLS) M27-A2 broth microdilution-based reference methods. More than 96% of isolates found to be susceptible to fluconazole by the reference method were identified as susceptible by the agar-based methods. Lesser degrees of correlation with the reference method were seen for isolates identified as resistant by the agar-based methods. Interpretive categories are not available for voriconazole,

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Sharma, Sheetal, Preeti Srivastava, Anjali Kulshrestha, and Ameer Abbas. "Evaluation of different phenotypic methods for the detection of methicillin resistant Staphylococcus aureus and antimicrobial susceptibility pattern of MRSA." International Journal Of Community Medicine And Public Health 4, no. 9 (2017): 3297. http://dx.doi.org/10.18203/2394-6040.ijcmph20173832.

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Background: Rapid and accurate detection of methicillin resistant Staphylococcus aureus (MRSA) is an important role of clinical microbiology laboratories to avoid treatment failure. The aim of this study was to compare conventional methods against the cefoxitin disc diffusion method to determine the best phenotypic method. Methods: Study was carried out in the Department of Microbiology, National Institute of Medical Sciences & Research, Jaipur (India), between July 2016 – December 2016. The methods included were Oxacillin E-test MIC, Oxacillin screen agar, Oxacillin disk diffusion, Cefoxi

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K., Mohana Priya, and Kouser Ali Shabana. "Antibacterial Activity of Aqueous Extract of Sea Weed Ulva Fasciata : An In Vitro Study." International Journal of Current Pharmaceutical Review and Research 2, no. 2 (2011): 102–5. https://doi.org/10.5281/zenodo.12698744.

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Ulva fasciata is a common sea weed and known for various medicinal properties. The aim ofthe present study was to screen the antimicrobial activity of Ulva fasciata against clinicalisolates of bacteria. The aqueous extract of the U. fasciata was studied for its antagonisticactivity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, andKlebsiella pneumoniae. In vitro antimicrobial activity was performed by Agar well diffusionmethod in Muller Hinton agar. The extract showed significant effect on the tested organisms.The extract showed maximum zone of inhibition against E. c

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Bamazi, Bitang, Agnassim Banito, Lankondjoa Kolani, Tchein Nimblika, Koffi Koba, and Komla Sanda. "In vitro evaluation of plant-extracts activity on two tomato pathogenic fungi in Togo." Net Journal of Agricultural Science 10, no. 4 (2022): 90–94. http://dx.doi.org/10.30918/njas.104.22.023.

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The effects of plant-based formulation on two common fungi of tomato were carried out in vitro. A total of five concentrations of the formulation: 0.2, 0.4, 0.6, 0.8 and 1% were tested using agar dilution and agar diffusion methods. Significant antifungal effects were observed. The plant-based extract formulation was more effective with the agar dilution method in comparison to the diffusion one and also depend on the higher concentrations. The antifungal effects decreased with the incubation time and a high effect was recorded after three days of incubation with mycelial growth inhibition fro

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Vulcani, Valcinir Aloisio Scalla, Maria Trindade Marques Bizarria, Marcos Akira d’Ávila, Lucia Helena Innocentini Mei, Cláudia Bernal, and Janice Rodrigues Perussi. "Cytotoxicity tests for nanostructured chitosan/PEO membranes using the agar diffusion method." Materials Research 15, no. 2 (2012): 213–17. http://dx.doi.org/10.1590/s1516-14392012005000013.

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22

Bonev, B., J. Hooper, and J. Parisot. "Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method." Journal of Antimicrobial Chemotherapy 61, no. 6 (2008): 1295–301. http://dx.doi.org/10.1093/jac/dkn090.

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Drugeon, H. B., M. E. Juvin, J. Caillon, and A. L. Courtieu. "Assessment of formulas for calculating critical concentration by the agar diffusion method." Antimicrobial Agents and Chemotherapy 31, no. 6 (1987): 870–75. http://dx.doi.org/10.1128/aac.31.6.870.

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Chang, Woo-Jin, Yoon-Hee Moon, and Yoon-Mo Koo. "Mathematical modeling for the estimation of lipase activity by agar diffusion method." Korean Journal of Chemical Engineering 14, no. 3 (1997): 179–83. http://dx.doi.org/10.1007/bf02706092.

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Pazur, John H. "Coupled gel electrophoresis-agar diffusion method for the detection of tumor antigens." Journal of Chromatography B: Biomedical Sciences and Applications 663, no. 1 (1995): 51–57. http://dx.doi.org/10.1016/0378-4347(94)00421-z.

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Muhammad Touqeer Hanif, Farhan Rasheed, Zainab Yousaf, Dania Niaz, Sara Arif, and Afshan Zia. "Diagnostic accuracy of MRSA chrom agar comparing it with cefoxitin disc diffusion method." Professional Medical Journal 32, no. 06 (2025): 664–69. https://doi.org/10.29309/tpmj/2025.32.06.9188.

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Objective: To evaluate the sensitivity and specificity of MeReSa Chrom agar to detect methicillin-resistant Staphylococcus aureus in clinical specimens. Study Design: Cross sectional. Setting: Department of Microbiology, Allama Iqbal Medical College, Jinnah Hospital Lahore. Period: January 2024 to January 2025. Methods: The 525 iolates of Staphylococcus aureus was isolated from various clinical samples. Cefoxitin disc diffusion method was used to isolate MRSA. All isolates were inoculated on Chrom agar, and growth was noted after 24 and 48 hours of incubation. All isolates were later analyzed

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Muravyeva, Victoria B., Natalya I. Soboleva, Oleg A. Makhlis, Vladimir O. Bondarenko, and Vasily I. Dorozhkin. "COMPARATIVE CHARACTERISTICS OF METHODS FOR DETERMINING TYLOSIN BY DIFFUSION IN AGAR." Problems of veterinary sanitation, hygiene and ecology 1, no. 41 (2022): 93–98. http://dx.doi.org/10.36871/vet.san.hyg.ecol.202201011.

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The article presents the features of conducting experiments on different microbiological media using microbial cultures. Optimal experimental conditions were selected for determining the activity of tylosin in antimicrobial drugs by diffusion into agar. The test microorganism, solvents, buffer solution, nutrient medium and test conditions for achieving a given goal are determined. Determination of the antimicrobial activity of tylosin by the microbiological method is not available in domestic sources. Tylosin is widely used in animal husbandry. As a result of the conducted studies, it was foun

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Hsieh, Bo-Young, Wei-Hsun Chao, Yi-Jing Xue, and Jyh-Mirn Lai. "A Ketoconazole Susceptibility Test for Malassezia pachydermatis Using Modified Leeming–Notman Agar." Journal of Fungi 4, no. 4 (2018): 126. http://dx.doi.org/10.3390/jof4040126.

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The aim of this study was to establish a ketoconazole susceptibility test for Malassezia pachydermatis using modified Leeming–Notman agar (mLNA). The susceptibilities of 33 M. pachydermatis isolates obtained by modified CLSI M27-A3 method were compared with the results by disk diffusion method, which used different concentrations of ketoconazole on 6 mm diameter paper disks. Results showed that 93.9% (31/33) of the minimum inhibitory concentration (MIC) values obtained from both methods were similar (consistent with two methods within 2 dilutions). M. pachydermatis BCRC 21676 and Candida parap

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Srinivasan, R., D. Natarajan, and M. S. Shivakumar. "Antimicrobial and GC-MS Analysis of Memecylon edule Leaf Extracts." International Journal of Current Pharmaceutical Review and Research 5, no. 1 (2014): 1–13. https://doi.org/10.5281/zenodo.12689427.

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Memecylon edule (L.) is belongs to Melastomataceae family used in many folkloremedicines. The present study was focused on different solvent (methanol, acetone, ethylacetate, chloroform and hexane) leaf extracts of M. edule were evaluated for theirantimicrobial potential and bioactive components (ethyl acetate extract) were analyzed byGC-MS. The antimicrobial activity was done by well in agar and disc diffusion methodsagainst four fungal pathogens and 17 bacterial pathogens include 5 reference (MTCC) strains. `The results show all these extracts exhibited different degree of antimicrobial acti

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Kosilova, I. S., L. V. Domotenko, N. K. Fursova, S. V. Dentovskaya, M. G. Ershova, and A. P. Shepelin. "TRIALS OF THE DOMESTICALLY PRODUCED NUTRIENT MEDIUM «AGAR MULLER-HINTON II - OBOLENSK»." Russian Clinical Laboratory Diagnostics 64, no. 6 (2019): 360–67. http://dx.doi.org/10.18821/0869-2084-2019-64-6-360-367.

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The results of the comparative tests of the «Agar Muller-Hinton II - Obolensk» nutrient medium developed in SRCAMB, Obolensk, and the control nutrient medium imported «Mueller Hinton II Agar» are presented in the study. The susceptibility of bacterial clinical strains to antimicrobial agents (AMP) was determined by the disc diffusion method and the method of gradient diffusion (E-test). The carbapenemase activity of the strains carrying the carbapenemase genes was determined by CIM-test. Total 173 characterized bacterial strains of species Klebsiella pneumoniae, Pseudomonas aeruginosa, Acineto

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Ali, Zainab Aziz, Thamer M. Jasim, and Widad M. K. Alani. "Antibacterial Activity of Chloroform Extract from Tagetes Erecta L. Flowers." Al Mustansiriyah Journal of Pharmaceutical Sciences 19, no. 4 (2019): 7–15. http://dx.doi.org/10.32947/ajps.v19i4.627.

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Antibacterial activity of chloroform of T. erecta flower extract was performed on six-gram negative bacteria species (Serratia marcescens, Acinobacter Spp., Escherichia coli, Salmonella Spp., Morganella morganii, Pseudomonas aeruginosa) and two-gram positive Bac-  teria (Streptococcus pneumonia and Staphylococcus aureus). The antibacterial study was performed by ager well diffusion and disk diffusion methods. The extract shows good results against examined bacteria and the results obtained by agar well diffusion method was better than those obtained from disk diffusion method.

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HARRIS, P. L., S. L. CUPPETT, and L. B. BULLERMAN. "A Technique Comparison of Isolation of Lipolytic Bacteria." Journal of Food Protection 53, no. 2 (1990): 176–77. http://dx.doi.org/10.4315/0362-028x-53.2.176.

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Three different agar diffusion screening methods, employing two growth media, standard plate count agar (SPCA) and 1% peptone enriched trypticase soy agar (TSA), were used to isolate psychrotrophic bacteria from raw milk which produce lipase. Clarified butterfat and nile blue sulfate served as the substrate and dye, respectively, in all methods. Although a double-overlay method yielded slightly greater numbers of lipolytic bacteria than a single-layered method with comparable media, better visual clarity was achieved with the single-layered method. Peptone enriched TSA medium supported better

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Lo-Ten-Foe, Jerome R., Anne Marie G. A. de Smet, Bram M. W. Diederen, Jan A. J. W. Kluytmans, and Peter H. J. van Keulen. "Comparative Evaluation of the VITEK 2, Disk Diffusion, Etest, Broth Microdilution, and Agar Dilution Susceptibility Testing Methods for Colistin in Clinical Isolates, Including Heteroresistant Enterobacter cloacae and Acinetobacter baumannii Strains." Antimicrobial Agents and Chemotherapy 51, no. 10 (2007): 3726–30. http://dx.doi.org/10.1128/aac.01406-06.

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ABSTRACT Increasing antibiotic resistance in gram-negative bacteria has recently renewed interest in colistin as a therapeutic option. The increasing use of colistin necessitates the availability of rapid and reliable methods for colistin susceptibility testing. We compared seven methods of colistin susceptibility testing (disk diffusion, agar dilution on Mueller-Hinton [MH] and Isosensitest agar, Etest on MH and Isosensitest agar, broth microdilution, and VITEK 2) on 102 clinical isolates collected from patient materials during a selective digestive decontamination or selective oral decontami

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Fagheei Aghmiyuni, Zeinab, Horieh Saderi, Parviz Owlia, and Navid Saidi. "Evaluation of the Effect of Lactobacillus acidophilus ATCC 4356 Bacteriocin against Staphylococcus aureus." BioMed Research International 2024 (March 22, 2024): 1–5. http://dx.doi.org/10.1155/2024/4119960.

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Background. Lactobacillus acidophilus is lactic acid bacteria that produce bacteriocins. Bacteriocins are antimicrobial peptides or proteins that exhibit activity against closely related bacteria. The aim of this study was to determine the effect of L. acidophilus ATCC 4356 bacteriocin against Staphylococcus aureus. Material and Methods. We used four different phenotypic methods for antimicrobial activities against two standard strains: methicillin-resistant S. aureus (MRSA) ATCC 33591 and methicillin-susceptible S. aureus (MSSA) ATCC 25923. The methods were (1) agar well diffusion, (2) overla

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Anandkumar H, Jyothi U S, and Rajeshwari R S. "Evaluation of cefoxitin disc diffusion test as a rapid phenotypic method for detection of methicillin resistance." Indian Journal of Microbiology Research 8, no. 3 (2021): 215–18. http://dx.doi.org/10.18231/j.ijmr.2021.044.

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: Accuracy and promptness in the detection of methicillin resistance are of key importance in ensuring correct antibiotic treatment in infected patients and control of MR staphylococci in the hospital environment. The aim of this study was to detect MRSA phenotypically by oxacillin screen agar and Oxacillin MIC method and to evaluate cefoxitin disc diffusion test as a screening tool for MRSA detection. In the present study, a total of 50 isolates of from various clinical samples collected were used for the detection of Methicillin resistant (MRSA). Methicillin resistance was determined by oxac

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Planche, Virginie, Sebastien Ducroz, Alexandre Alanio, Marie-Elisabeth Bougnoux, Olivier Lortholary, and Eric Dannaoui. "In VitroCombination of Anidulafungin and Voriconazole against Intrinsically Azole-Susceptible and -Resistant Aspergillus spp." Antimicrobial Agents and Chemotherapy 56, no. 8 (2012): 4500–4503. http://dx.doi.org/10.1128/aac.00045-12.

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ABSTRACTIn vitrointeraction of anidulafungin with voriconazole was tested by a microdilution broth checkerboard technique and an agar diffusion method against 30Aspergillusclinical isolates belonging to five different species. By using a complete inhibition endpoint, indifferent interactions were observed for 97% of the isolates by the checkerboard technique (FIC index from 0.5 to 2) and for 100% of the isolates by the agar diffusion method (variation of −2 to +1 log2dilutions).

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Zhou, Ying, Naijin Xu, Meijuan Zhang, and Hui Liu. "A New Method for Measuring Total Antioxidant Capacity in Urine Using the Iodine Starch Agar Based on Agar diffusion." Current Analytical Chemistry 12, no. 5 (2016): 425–30. http://dx.doi.org/10.2174/1573411011666150903204210.

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Bhatta, Sabita, Babli Basu, Chandrasekhar Narharrao Chaudhary, and Ashok Kumar Praharaj. "In Vitro Antimicrobial Activity of Tigecycline." Medical Journal of Shree Birendra Hospital 13, no. 2 (2015): 35–38. http://dx.doi.org/10.3126/mjsbh.v13i2.13114.

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Introduction: Tigecycline is a novel glycylcycline derivative of the tetracycline with activity against a wide range of organisms including Methicillin resistant Staphylococcus aureus, Vancomycin resistant Enterococcus , Extended spectrum beta lactamase producing (Escherichia coli , Klebsiella pneumonia) and Acinetobacter species. The aim of the study was to assess effectiveness of the drug against methicillin resistant Staphylococcus aureus (MRSA), vancomycin resistant enterococci (VRE), ESBL producers and carbapenem resistant Acinetobacter baumannii and to compare the efficacy of different m

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Abdullah, Abulkhair, Muhammad Subhan A. Sibadu, and Mutmainnah Mutmainnah. "Aktivitas Antibakteri Kokristal Sefiksim-Nikotinamida Menggunakan Agar Diffusion dan Broth Dilution Test." Jurnal Sains dan Kesehatan 5, no. 6 (2023): 886–92. http://dx.doi.org/10.25026/jsk.v5i6.2069.

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Cefixime has been formulated in cocrystal form with nicotinamide (coformer). SEF-NIK cocrystals were prepared at a mole ratio of 1:1 using a liquid-assisted grinding method. The cocrystals were characterized using DSC, FTIR, SEM, and PXRD. The characterization results showed that cocrystals were formed between cefixime and nicotinamide. The results of the solubility test, dissolution rate and permeability of the SEF-NIK cocrystal showed a significant increase compared to pure cefixime. Based on these results, the SEF-NIK cocrystal was continued for pharmacodynamic studies with in vitro antibac

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Hinton Jr., A., and K. D. Ingram. "Comparison of the Antibacterial Activity of Chelating Agents Using the Agar Diffusion Method." International Journal of Poultry Science 9, no. 11 (2010): 1023–26. http://dx.doi.org/10.3923/ijps.2010.1023.1026.

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Tankhiwale, Supriya, Sunita Gajbhiye, and Rajaram Powar. "FLUCONAZOLE SUSCEPTIBILITY TESTING OF CANDIDA SPECIES BY DISC DIFFUSION AND AGAR DILUTION METHOD." Journal of Evolution of Medical and Dental Sciences 1, no. 4 (2012): 527–31. http://dx.doi.org/10.14260/jemds/83.

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Ciftci, Nurullah, Betul Cetin, Ugur Arslan, and Hatice Turk-Dagi. "Comparison of AST Fast ES/NF Chromogenic Agar with the Disk Diffusion Method." Klimik Dergisi/Klimik Journal 37, no. 4 (2024): 252–55. https://doi.org/10.36519/kd.2024.5040.

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Thomsen, V. Frølund. "CORRELATION OF THE PLATE-DILUTION METHOD TO THE AGAR DIFFUSION METHOD (DISC-AND TABLET METHODS) WITH A SPECIAL VIEW TO THE IMPORTANCE OF PRE-DIFFUSION." Acta Pathologica Microbiologica Scandinavica 54, no. 1 (2009): 107–20. http://dx.doi.org/10.1111/j.1699-0463.1962.tb01228.x.

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Muravyeva, Victoria B., Natalya I. Soboleva, Artem V. Zavyalov, Oleg A. Makhlis, Tatyana N. Likhikh, and Irina V. Kis. "Comparative characteristics of quantitative methods for the determination of flavophospholipol in medicinal preparations by the agar diffusion method." Veterinariya, Zootekhniya i Biotekhnologiya 12/1, no. 121 (2023): 23–28. http://dx.doi.org/10.36871/vet.zoo.bio.202312103.

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Flavophospholipol is a phosphoglycolipid antibiotic that inhibits the development of gram-positive microorganisms in the gastrointestinal tract, slows down the growth of gram-negative bacteria Salmonella spp. and E.coli, without having a negative effect on lactobacilli and bifidobacteria, helps to reduce the extrachromosomal resistance of microorganisms to antibacterial agents, increasing their effectiveness. Preparations based on flavophospholipol are used for piglets and pigs, cattle and calves, rabbits and fur-bearing animals, poultry for prophylactic purposes in case of bacterial diseases,

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Sabera.Aliasgar, Rangwala, and Mahendra Desai Deepa. "Antibacterial activity of some common species used in indian kitchen and effect of it in combination." World Journal of Advanced Research and Reviews 16, no. 1 (2022): 084–87. https://doi.org/10.5281/zenodo.7768996.

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Identification of traditional remedies used in Indian household as antibacterial agent. A study was undertaken to document antibacterial activity of Curcumin. It is the most abundant polyphenol present in the dietary spice turmeric. Turmeric powder, derived from the rhizome of Curcuma longa, which is associated to the Zingberiaceae family, and the antibacterial activity and medicinal properties of ginger extracts derived from <em>zingerber officianale </em>were studied by agar diffusion method and then the combined effect of both this herbal drug in various concentration was studied

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Sabera.Aliasgar, Rangwala, and Mahendra Desai Deepa. "Antibacterial activity of some common species used in indian kitchen and effect of it in combination." World Journal of Advanced Research and Reviews 16, no. 1 (2022): 084–87. https://doi.org/10.5281/zenodo.7769075.

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Identification of traditional remedies used in Indian household as antibacterial agent. A study was undertaken to document antibacterial activity of Curcumin. It is the most abundant polyphenol present in the dietary spice turmeric. Turmeric powder, derived from the rhizome of Curcuma longa, which is associated to the Zingberiaceae family, and the antibacterial activity and medicinal properties of ginger extracts derived from <em>zingerber officianale </em>were studied by agar diffusion method and then the combined effect of both this herbal drug in various concentration was studied

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Farias, DF, AFU Carvalho, CC Oliveira, et al. "Alternative method for quantification of alfa-amylase activity." Brazilian Journal of Biology 70, no. 2 (2010): 405–7. http://dx.doi.org/10.1590/s1519-69842010005000008.

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A modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL-1. Clear radial diffusion zones were measured after 4 hours of incubation at 20 Â°C. A linear relationship between the logarithm of enzyme activities and the area of clear zones was obtained. The method was validated by testing α-amyl

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Ali Khan, Mohsin, Shadma Yaqoob, and Sharique Ahmad. "Antimicrobial Activity of Azadirachta indica, against Target Pathogens and Its Utility as a Disinfectant and Floor Cleaner." Journal of Evolution of Medical and Dental Sciences 10, no. 25 (2021): 1899–903. http://dx.doi.org/10.14260/jemds/2021/392.

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BACKGROUND Azadirachta indica, commonly known as neem, neem tree or Indian lilac, Limbo, Nim, Nimba, Medusa and Vempu. It is typically grown in tropical and subtropical regions. Neem belongs to family meliaceae. Neem is a native tree of India. It is also called “village pharmacy of south Asia” (India) because of its enormous medicinal properties and people use it most of the time without knowing its multiple advantages. This study was done to know the efficacy of medicinal plant named Azadirachta indica (Neem) against pathogenic microorganisms and about its utility as disinfectant and floor cl

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Khan, Md Mottalib Hossain, Md Ahsanul Haque, Md Shah Alam, Farjana Kabir, Md Azraf Hossain, and MM Washee Parvez. "Agar-Based Disc Diffusion Assay for Susceptibility Testing of Dermatophytes in the Rajshahi Region." TAJ: Journal of Teachers Association 35, no. 1 (2022): 99–106. http://dx.doi.org/10.3329/taj.v35i1.61164.

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Background: Dermatophytosis is considered one of the most common public health problems in the world and is the most commonly diagnosed skin disease in Bangladesh. The prevalence and types of dermatophyte infections vary with climate conditions, age, lifestyle, and population migration patterns. Depending upon the anatomical site of the lesion, dermatophytes are classified into different varieties. Objective: To isolate dermatophytes and their antifungal susceptibility pattern by agar-based disc diffusion method in the Rajshahi region. Materials and Methods: Clinical samples (e.g., skin

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Hill, Bertha C., Carolyn N. Baker, and Fred C. Tenover. "A Simplified Method for Testing Bordetella pertussisfor Resistance to Erythromycin and Other Antimicrobial Agents." Journal of Clinical Microbiology 38, no. 3 (2000): 1151–55. http://dx.doi.org/10.1128/jcm.38.3.1151-1155.2000.

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Present methods of antimicrobial susceptibility testing ofBordetella pertussis are time consuming and require specialized media that are not commercially available. We tested 52 isolates of B. pertussis for resistance to erythromycin, trimethoprim-sulfamethoxazole, chloramphenicol, and rifampin by agar dilution with Bordet-Gengou agar (BGA) containing 20% horse blood (reference method), Etest using BGA and Regan-Lowe agar without cephalexin (RL−C), and disk diffusion using BGA and RL−C. The organisms tested included four erythromycin-resistant isolates ofB. pertussis from a single patient, a s

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