Relevant bibliographies by topics / Agar overlay method

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Academic literature on the topic 'Agar overlay method'

Author: Grafiati
Published: 4 June 2021
Last updated: 15 February 2022

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Journal articles on the topic "Agar overlay method"

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SCHMALZ, G. "Agar overlay method." International Endodontic Journal 21, no. 2 (September 25, 2007): 59–66. http://dx.doi.org/10.1111/j.1365-2591.1988.tb00956.x.

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YAN, ZHINONG, JOSHUA B. GURTLER, and JEFFREY L. KORNACKI. "A Solid Agar Overlay Method for Recovery of Heat-Injured Listeria monocytogenes." Journal of Food Protection 69, no. 2 (February 1, 2006): 428–31. http://dx.doi.org/10.4315/0362-028x-69.2.428.

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A solid agar overlay method was developed for recovery of heat-injured Listeria monocytogenes. Presolidified nonselective tryptic soy agar with 0.6% yeast extract (TSAYE, 2% agar) was overlaid on top of solidified modified Oxford agar (MOX). Heat injury of L. monocytogenes was conducted at 58°C for 6 min in a jacketed flask filled with tryptic soy broth. Both noninjured and heat-treated L. monocytogenes cells were plated onto TSAYE, MOX, and TSAYE-MOX plates. No significant differences (P > 0.05) in recovery were found among the three media for noninjured bacterial cells. Recovery of heat-injured L. monocytogenes cells on TSAYE-MOX overlay plates was equivalent to that on the nonselective TSAYE medium, whereas recovery on the selective MOX medium was significantly lower (P < 0.05) compared with both TSAYE and the overlay plates. There were no significant differences (P > 0.05) among the overlay plates prepared 0, 2, 4, 6, 8, 16, and 24 h prior to plating heat-injured bacterial cells. The TSAYE-MOX overlay also allowed differentiation of L. monocytogenes from a mixture of four other types of foodborne pathogens. This solid agar overlay method for recovery of heat-injured L. monocytogenes cells is less time-consuming and less complicated than the conventional overlay-underlay technique and the double overlay modification of the thin agar layer method and may allow for greater laboratory plating efficiencies.
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Parreira, Valéria Regina, Clarice Weis Arns, and Tomomasa Yano. "An agar-overlay method for detection of toxins produced byEscherichia coli." FEMS Microbiology Letters 120, no. 3 (July 1994): 303–6. http://dx.doi.org/10.1111/j.1574-6968.1994.tb07050.x.

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BACK, KYEONG-HWAN, SANG-OH KIM, KI-HWAN PARK, MYUNG-SUB CHUNG, and DONG-HYUN KANG. "Spray Method for Recovery of Heat-Injured Salmonella Typhimurium and Listeria monocytogenes." Journal of Food Protection 75, no. 10 (October 1, 2012): 1867–72. http://dx.doi.org/10.4315/0362-028x.jfp-11-512.

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Selective agar is inadequate for supporting recovery of injured cells. During risk assessment of certain foods, both injured and noninjured cells must be enumerated. In this study, a new method (agar spray method) for recovering sublethally heat-injured microorganisms was developed and used for recovery of heat-injured Salmonella Typhimurium and Listeria monocytogenes. Molten selective agar was applied as an overlay to presolidified nonselective tryptic soy agar (TSA) by spray application. Heat-injured cells (55°C for 10 min in 0.1% peptone water or 55°C for 15 min in sterilized skim milk) were inoculated directly onto solidified TSA. After a 2-h incubation period for cell repair, selective agar was applied to the TSA surface with a sprayer, and the plates were incubated. The recovery rate for heat-injured Salmonella Typhimurium and L. monocytogenes with the spray method was compared with the corresponding rates associated with TSA alone, selective media alone, and the conventional overlay method (selective agar poured on top of resuscitated cells grown on TSA and incubated for 2 h). No significant differences (P > 0.05) were found in pathogen recovery obtained with TSA, the overlay method, and the spray method. However, a lower recovery rate (P < 0.05) was obtained for isolation of injured cells on selective media. Overall, these results indicate that the agar spray method is an acceptable alternative to the conventional overlay method and is a simpler and more convenient approach to recovery and detection of injured cells.
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Drijber, R. A., and W. B. McGill. "A modification of the method using cellulose overlay agar to isolate and purify cellulolytic cytophagas from enrichment culture." Canadian Journal of Microbiology 38, no. 7 (July 1, 1992): 687–89. http://dx.doi.org/10.1139/m92-111.

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We report here on a modification of the cellulose overlay agar method for isolating and purifying cellulolytic cytophagas from enrichment cultures. We call it the "back-door" method, and it overcomes two existing problems. First, it prevents recontamination with organisms growing on the agar surface. Second, it permits purification of cellulolytic cytophagas that are unable to utilize glucose or that are accompanied by a rapidly growing contaminant with spreading habit. Pure cultures of cellulolytic cytophagas were obtained from five enrichment cultures of nine soil samples examined. Two limitations are that (i) the cytophaga must be able to penetrate the overlay and (ii) some noncellulolytic cytophagas may also penetrate the overlay to yield cocultures. In conclusion, the back-door method can be used both to isolate and purify cellulolytic cytophagas from soils, using only a cellulose-based medium. Key words: back-door method, cellulolytic cytophagas, cellulose overlay agar, cellulolysis.
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노태희, 이승연, Jeong Moon-Jin, and 이명화. "Cytotoxicity comparison of titanium by agar overlay method for various cell line applications." Korean Journal of Oral Anatomy 38, no. 1 (December 2017): 1–11. http://dx.doi.org/10.35607/kjoa.38.1.201712.001.

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Mackenzie, A. M., and R. L. Rivera-Calderon. "Agar overlay method to measure adherence of Staphylococcus epidermidis to four plastic surfaces." Applied and Environmental Microbiology 50, no. 5 (1985): 1322–24. http://dx.doi.org/10.1128/aem.50.5.1322-1324.1985.

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Nuthan, Bettadapura Rameshgowda, Devaraju Rakshith, Kuppuru Mallikarjunaiah Marulasiddaswamy, H. C. Yashavantha Rao, Kolathur Puttamadaiah Ramesha, Nagabhushana Chandra Mohana, Shiva Siddappa, Doreraj Darshan, Kigga Kaadappa Sampath Kumara, and Sreedharamurthy Satish. "Application of Optimized and Validated Agar Overlay TLC–Bioautography Assay for Detecting the Antimicrobial Metabolites of Pharmaceutical Interest." Journal of Chromatographic Science 58, no. 8 (August 7, 2020): 737–46. http://dx.doi.org/10.1093/chromsci/bmaa045.

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Abstract The agar overlay TLC–bioautography is one of the crucial methods for simultaneous in situ detection and separation of antimicrobial metabolites of pharmaceutical interest. The main focus of this research relies on the dereplication of an antimicrobial metabolite coriloxin derived from mycoendophytic Xylaria sp. NBRTSB-20 with a validation of agar overlay TLC–bioautography technique. This polyketide metabolite coriloxin was purified by column chromatography, and its purity was assessed by HPLC, UPLC-ESI-QTOF-MS, FT-IR and NMR spectral analysis. The antimicrobial capability of ethyl acetate extract and the purified compound coriloxin was determined by disc diffusion, minimal inhibitory concentration and agar overlay TLC–bioautography assay. The visible LOD of coriloxin antimicrobial activity was found at 10 μg for Escherichia coli and 20 μg for both Staphylococcus aureus and Fusarium oxysporum. Inter- and intra-day precision was determined as the relative standard deviation is less than 6.56%, which proved that this method was precise. The accuracy was expressed as recovery, and the values were found ranging from 91.18 to 108.73% with RSD values 0.94–2.30%, respectively. The overall findings of this investigation suggest that agar overlay TLC–bioautography assay is a suitable and acceptable method for the in situ determination of antimicrobial pharmaceuticals.
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Kang, D. H., and G. R. Siragusa. "Agar Underlay Method for Recovery of Sublethally Heat-Injured Bacteria." Applied and Environmental Microbiology 65, no. 12 (December 1, 1999): 5334–37. http://dx.doi.org/10.1128/aem.65.12.5334-5337.1999.

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ABSTRACT A method of recovering sublethally heat-injured bacteria was developed. The procedure (termed the agar underlay method) uses a nonselective agar underlaid with a selective medium. In a two-chambered petri dish, the Lutri plate (LP), a nonselective agar is inoculated with a population of sublethally heat-injured bacteria. After a 2-h repair incubation period, selective agar is added to the bottom chamber of the LP and incubated. By diffusing through the nonselective top agar, selective agents from the underlay medium impart selectivity to the system. By the agar underlay method, recovery rates of the heat-injured food-borne pathogens Escherichia coli O157:H7 and Salmonella typhimurium were not different (P > 0.05) from recovery rates determined with nonselective media. Sublethally heat-injured cells (60°C for 1.5 min in buffer or 80°C for 30 s on meat surfaces) grew and produced a typical colony morphology and color reaction when the agar underlay procedure was used with the appropriate respective selective agars. Unlike agar overlay methods for injury repair, the agar underlay procedure allows the typical selective-medium colony morphology to develop and allows colonies to be more easily picked for further characterization. Higher recovery rates of heat-injured fecal enterococci from bovine fecal samples and total coliforms from animal waste lagoons were obtained by the agar underlay method with selective agars than by direct plating on the respective selective media.
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Rahalison, L., M. Hamburger, K. Hostettmann, M. Monod, and E. Frenk. "A bioautographic agar overlay method for the detection of antifungal compounds from higher plants." Phytochemical Analysis 2, no. 5 (November 1991): 199–203. http://dx.doi.org/10.1002/pca.2800020503.

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Dissertations / Theses on the topic "Agar overlay method"

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McCanna, David. "Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products." Thesis, 2009. http://hdl.handle.net/10012/4338.

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The utilization of in vitro tests with a tiered testing strategy for detection of mild ocular irritants can reduce the use of animals for testing, provide mechanistic data on toxic effects, and reduce the uncertainty associated with dose selection for clinical trials. The first section of this thesis describes how in vitro methods can be used to improve the prediction of the toxicity of chemicals and ophthalmic products. The proper utilization of in vitro methods can accurately predict toxic threshold levels and reduce animal use in product development. Sections two, three and four describe the development of new sensitive in vitro methods for predicting ocular toxicity. Maintaining the barrier function of the cornea is critical for the prevention of the penetration of infections microorganisms and irritating chemicals into the eye. Chapter 2 describes the development of a method for assessing the effects of chemicals on tight junctions using a human corneal epithelial and canine kidney epithelial cell line. In Chapter 3 a method that uses a primary organ culture for assessing single instillation and multiple instillation toxic effects is described. The ScanTox system was shown to be an ideal system to monitor the toxic effects over time as multiple readings can be taken of treated bovine lenses using the nondestructive method of assessing for the lens optical quality. Confirmations of toxic effects were made with the utilization of the viability dye alamarBlue. Chapter 4 describes the development of sensitive in vitro assays for detecting ocular toxicity by measuring the effects of chemicals on the mitochondrial integrity of bovine cornea, bovine lens epithelium and corneal epithelial cells, using fluorescent dyes. The goal of this research was to develop an in vitro test battery that can be used to accurately predict the ocular toxicity of new chemicals and ophthalmic formulations. By comparing the toxicity seen in vivo animals and humans with the toxicity response in these new in vitro methods, it was demonstrated that these in vitro methods can be utilized in a tiered testing strategy in the development of new chemicals and ophthalmic formulations.
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Book chapters on the topic "Agar overlay method"

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Kropinski, Andrew M., Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr, and Roger P. Johnson. "Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay." In Methods in Molecular Biology, 69–76. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-164-6_7.

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Fukui, Yoshio, Shigehiko Yumura, Toshiko K. Yumura, and Hiroshi Mori. "[54] Agar overlay method: High-resolution immunofluorescence for the study of the contractile apparatus." In Structural and Contractile Proteins Part C: The Contractile Apparatus and the Cytoskeleton, 573–80. Elsevier, 1986. http://dx.doi.org/10.1016/0076-6879(86)34122-3.

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Fukui, Yoshio, Shigehiko Yumura, and Toshiko K. Yumura. "Chapter 19 Agar-Overlay Immunofluorescence: High-Resolution Studies of Cytoskeletal Components and Their Changes during Chemotaxis." In Methods in Cell Biology, 347–56. Elsevier, 1987. http://dx.doi.org/10.1016/s0091-679x(08)61655-6.

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Tejaswini, S., N. Sriraam, and Pradeep G. C. M. "Identification of High Risk and Low Risk Preterm Neonates in NICU." In Biomedical and Clinical Engineering for Healthcare Advancement, 119–40. IGI Global, 2020. http://dx.doi.org/10.4018/978-1-7998-0326-3.ch007.

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Infant cries are referred as the biological indicator where infant distress is expressed without any external stimulus. One can assess the physiological changes through cry characteristics that help in improving clinical decision. In a typical Neonatal Intensive Care Unit (NICU), recognizing high-risk and low-risk admitted preterm neonates is quite challenging and complex in nature. This chapter attempts to develop pattern recognition-based approach to identify high-risk and low-risk preterm neonates in NICU. Four clinical conditions were considered: two Low Risk (LR) and two High Risk (HR), LR1- Appropriate Gestational Age (AGA), LR2- Intrauterine Growth Restriction (IUGR), HR1-Respiratory Distress Syndrome (RDS), and HR2- Premature Rupture of Membranes (PROM). An overall cry unit of 800 (n=20 per condition) was used for the proposed study. After appropriate pre-processing, Bark Frequency Cepstral Coefficient (BFCC) was estimated using three methods. Schroeder, Zwicker and Terhardt; and Transmiller; and a non-linear Support Vector Machine (SVM) Classifier were employed to discriminate low-risk and high-risk groups. From the simulation results, it was observed that sensitivity specificity and accuracy of 91.47%, 91.42%, and 92.9% respectively were obtained using the BFCC estimated for classifying high risk and low risk with SVM classification.
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