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Journal articles on the topic 'Agar well diffusion method'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Ahuja, Chitrangana, Harpreet Kaur, and Rajeev Sharma. "Antibacterial Activity of Terminalia Chebula Fruit by Agar well Diffusion Method." Journal of Chemistry, Environmental Sciences and its Applications 1, no. 2 (2015): 67–72. http://dx.doi.org/10.15415/jce.2015.12006.

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Nurhayati, Lilih Siti, Nadhira Yahdiyani, and Akhmad Hidayatulloh. "PERBANDINGAN PENGUJIAN AKTIVITAS ANTIBAKTERI STARTER YOGURT DENGAN METODE DIFUSI SUMURAN DAN METODE DIFUSI CAKRAM." Jurnal Teknologi Hasil Peternakan 1, no. 2 (2020): 41. http://dx.doi.org/10.24198/jthp.v1i2.27537.

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Antibacterial activity testing can be done using the agar diffusion method, including agar well difussion and disk diffusion agar methods. This study aims to compare two antibacterial testing methods to analyze the anti-bacterial activity of a yogurt starter against the bacteria Eschericia coli and Staphilococcus aureus. The study was conducted experimentally with 5 concentrations of yogurt starter, namely 2%, 4%, 6%, 8%, and 10%. Testing antibacterial activity using two methods ,disk diffusion agar and well difussion agar methods. The research showed that agar well diffusion method obtained a

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Tarek, A. El-bashiti. "Production of Cephalosporin C from Acremonium chrysogenum, and its Antimicrobial Activity against Some Pathogenic Bacteria." Pharmaceutical and Chemical Journal 4, no. 2 (2017): 19–23. https://doi.org/10.5281/zenodo.13762620.

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The Production of cephalosporin C by <em>Acremonium chrysogenum</em>in flask contain fermentation medium, after that its extraction, purification and estimation was performed by Spectrophotometric Method. The antimicrobial activities of both the crude and purified cephalosporin C were performed against the test organisms by Agar Well Diffusion Method. Agar well diffusion method and the inhibition zones were recorded and evaluated the effectiveness of the antibiotic against gram positive bacteria (<em>Staphylococcus aereus</em> and <em>Bacillus subtilis</em>) and no effectiveness against gram n

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K., Mohana Priya, and Kouser Ali Shabana. "Antibacterial Activity of Aqueous Extract of Sea Weed Ulva Fasciata : An In Vitro Study." International Journal of Current Pharmaceutical Review and Research 2, no. 2 (2011): 102–5. https://doi.org/10.5281/zenodo.12698744.

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Ulva fasciata is a common sea weed and known for various medicinal properties. The aim ofthe present study was to screen the antimicrobial activity of Ulva fasciata against clinicalisolates of bacteria. The aqueous extract of the U. fasciata was studied for its antagonisticactivity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, andKlebsiella pneumoniae. In vitro antimicrobial activity was performed by Agar well diffusionmethod in Muller Hinton agar. The extract showed significant effect on the tested organisms.The extract showed maximum zone of inhibition against E. c

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Boyanova, Lyudmila, Galina Gergova, Rossen Nikolov, et al. "Activity of Bulgarian propolis against 94 Helicobacter pylori strains in vitro by agar-well diffusion, agar dilution and disc diffusion methods." Journal of Medical Microbiology 54, no. 5 (2005): 481–83. http://dx.doi.org/10.1099/jmm.0.45880-0.

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Propolis exhibits antimicrobial, anti-inflammatory and other biological effects. The aim of this study was to evaluate the activity of 30 % ethanolic extract of Bulgarian propolis against 94 Helicobacter pylori strains by three methods. By the agar-well diffusion method, only 13.8 % of the strains exhibited no inhibition by 30 μl propolis extract (containing 9 mg propolis) and all isolates were inhibited to some extent by 90 μl of the extract (27 mg propolis) per well. The mean diameters of growth inhibition by 30, 60 or 90 μl propolis extract or 30 μl 96 % ethanol per well were 16.8, 19.2, 27

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Srinivasan, R., D. Natarajan, and M. S. Shivakumar. "Antimicrobial and GC-MS Analysis of Memecylon edule Leaf Extracts." International Journal of Current Pharmaceutical Review and Research 5, no. 1 (2014): 1–13. https://doi.org/10.5281/zenodo.12689427.

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Memecylon edule (L.) is belongs to Melastomataceae family used in many folkloremedicines. The present study was focused on different solvent (methanol, acetone, ethylacetate, chloroform and hexane) leaf extracts of M. edule were evaluated for theirantimicrobial potential and bioactive components (ethyl acetate extract) were analyzed byGC-MS. The antimicrobial activity was done by well in agar and disc diffusion methodsagainst four fungal pathogens and 17 bacterial pathogens include 5 reference (MTCC) strains. `The results show all these extracts exhibited different degree of antimicrobial acti

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Kandukuri, Naga Hari Krishna, Kishore Babu M, Bhargavi M, et al. "Invitro antimicrobial activity of bitter melon leaf extract using well diffusion method." Journal of Modern Techniques in Biology and Allied Sciences 1, no. 1 (2024): 13–19. https://doi.org/10.70604/jmtbas.v1i1.10.

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The aim of present study examines the invitro antimicrobial properties of leaf removes from Momordica charantia, commonly known as bitter melon. Utilizing different extraction techniques, we arranged separates and considered their viability in contrast to a scope of pathogenic microorganisms, including microbes and growths. Using a paper plate dispersion test, its antimicrobial viability against Staphylococcus aureus, Salmonella typhi, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa was assessed using its methanolic extracts. The Agar Well-Dispe

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Megawati, Megawati, Mochammad Yuwono, and Riesta Primaharinastiti. "Antibacterial activity of Randu Honey against some bacterial pathogens using agar well diffusion method." Riset Informasi Kesehatan 14, no. 1 (2025): 49. https://doi.org/10.30644/rik.v14i1.954.

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Background: Honey's antibacterial and other therapeutic qualities have long been recognized because of its bioactive components, which include hydrogen peroxide, flavonoids, and phenolics. Randu honey is a monofloral honey that is made from the nectar of Ceiba pentandra and has the ability to suppress Gram-positive as well as Gram-negative bacteria. Because antibiotic resistance is on the rise, it is crucial to investigate alternative treatments like honey. Method: This study evaluates the antibacterial activity of randu honey using the agar well diffusion method or Kirby-Bauer diffusion metho

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Bhuvaneswari., M*and P. Sivagurunathan. "SCREENING OF BIOSURFACTANT PRODUCTION BY BACILLUS SP ISOLATED FROM COASTAL REGION IN CUDDALORE TAMILNADU." IAJPS,CSK PUBLICATIONS 03, no. 11 (2016): 1370–75. https://doi.org/10.5281/zenodo.200636.

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Marine microorganisms produce extracellular or membrane associated surface-active compounds (bio surfactants). Biosurfactant are organic compounds belonging to various classes including glycolipids, lipopeptides, fatty acids, phospholipids that reduce the interfacial tension between immiscible liquids.This study deals with production and characterization of biosurfactant from Bacillus sp. The efficiency of Bacillus spstrain isolated from a marine sediments soil sample from coastal region -Cuddalore. Methods: Bushnell Haas (BH) liquid medium was used as the enrichment medium with 1 %(v/v) diese

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Singh, R. "Pharmacological screening of heartwood of Tectona grandis Linn.for antibacterial activity by Agar-Well Diffusion method." IOP Conference Series: Materials Science and Engineering 1221, no. 1 (2022): 012019. http://dx.doi.org/10.1088/1757-899x/1221/1/012019.

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Abstract Medicinal plants has always been a subject of interest for human being from ancient times due to health benefits. Researchers found that, people in different regions of the world used the same or similar plants for health benefits. Medicinal plant research includes many fields like drug discovery, ethnobotany, traditional and indigenous medicines. During corona pandemic we realised that there are no effective therapies available for new and remerging infectious diseases. Public health badly affected due to drug resistant microorganism and appearance of new disease causing microbes. Du

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Ali, Zainab Aziz, Thamer M. Jasim, and Widad M. K. Alani. "Antibacterial Activity of Chloroform Extract from Tagetes Erecta L. Flowers." Al Mustansiriyah Journal of Pharmaceutical Sciences 19, no. 4 (2019): 7–15. http://dx.doi.org/10.32947/ajps.v19i4.627.

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Antibacterial activity of chloroform of T. erecta flower extract was performed on six-gram negative bacteria species (Serratia marcescens, Acinobacter Spp., Escherichia coli, Salmonella Spp., Morganella morganii, Pseudomonas aeruginosa) and two-gram positive Bac-  teria (Streptococcus pneumonia and Staphylococcus aureus). The antibacterial study was performed by ager well diffusion and disk diffusion methods. The extract shows good results against examined bacteria and the results obtained by agar well diffusion method was better than those obtained from disk diffusion method.

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Gummuluri, Sriram, Venkata Teja Kavalipurapu, and Apoorva Vasundhara Kaligotla. "Antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia Against Enterococcus Feacalis by Agar Well Diffusion Method and Minimal Inhibitory Concentration- An Invitro Study." Brazilian Dental Science 22, no. 3 (2019): 365–70. http://dx.doi.org/10.14295/bds.2019.v22i3.1731.

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Introduction: the long term success of root canal treatment is ultimately related to the effective debridement and disinfection of the root canal system. Hence, the irrigants play an important role in achieving the good penetrability and bactericidal activity. The present study was mainly aimed at evaluating the invitro antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia by agar well diffusion and minimal inhibitory concentration. Materials and Methods:the antimicrobial efficacy of the Novel Ethanolic Extract of Morinda Citrifolia was tested using agar well diffusion and mi

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Devillers, J., R. Steiman, and F. Seigle-Murandi. "The usefulness of the agar-well diffusion method for assessing chemical toxicity to bacteria and fungi." Chemosphere 19, no. 10-11 (1989): 1693–700. http://dx.doi.org/10.1016/0045-6535(89)90512-2.

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Er, Yesim, Nur Sivri, and Mustafa Mirik. "Antimicrobial Activity of Essential Oil Against Rhizobium (Agrobacterium) vitis Using Agar Well and Disc Diffusion Method." Bacteriology Journal 8, no. 1 (2017): 1–11. http://dx.doi.org/10.3923/bj.2018.1.11.

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Madhivathani S M, Umesh K, Sangeeta Chavan, Palanivel Pandian R, and Prem Kumar P. "Comparative evaluation of anti-bacterial effect of Caesalpinia sappan aqueous extract and 0.12% Chlorhexidine against Streptococcus mutans – An in vitro study." International Journal of Community Dentistry 11, no. 2 (2023): 61–68. http://dx.doi.org/10.56501/intjcommunitydent.v11i2.867.

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Aim and objectives: To evaluate and compare the antimicrobial activity of various concentration of C.sappan wood aqueous extract (100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, 500mg/ml and 600mg/ml) and 0.12% chlorhexidine mouthwash using agar well diffusion method against St.mutans. Materials and Methods: A comparative experimental invitro study was conducted to evaluate and compare antimicrobial activity of various concentration of C.Sappan aqueous extract and 0.12% Chlorhexidine. The study enclosed extract of 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, 500mg/ml, 600mg/ml and 0.12% Chlorhexidine as co

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Ali Khan, Mohsin, Shadma Yaqoob, and Sharique Ahmad. "Antimicrobial Activity of Azadirachta indica, against Target Pathogens and Its Utility as a Disinfectant and Floor Cleaner." Journal of Evolution of Medical and Dental Sciences 10, no. 25 (2021): 1899–903. http://dx.doi.org/10.14260/jemds/2021/392.

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BACKGROUND Azadirachta indica, commonly known as neem, neem tree or Indian lilac, Limbo, Nim, Nimba, Medusa and Vempu. It is typically grown in tropical and subtropical regions. Neem belongs to family meliaceae. Neem is a native tree of India. It is also called “village pharmacy of south Asia” (India) because of its enormous medicinal properties and people use it most of the time without knowing its multiple advantages. This study was done to know the efficacy of medicinal plant named Azadirachta indica (Neem) against pathogenic microorganisms and about its utility as disinfectant and floor cl

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Esra, Demir, and Başbülbül Gamze. "Screening of Bacteriocin Production in Lactic Acid Bacteria Isolated From Fermented Dairy Products." Biotechnology Journal International 18, no. 2 (2017): 1–9. https://doi.org/10.9734/BJI/2017/33504.

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<strong>Aims: </strong>In this study we aimed to screen LAB strains from different fermented dairy products by phenotypic and genotypic methods. <strong>Methodology:</strong> 138 LAB isolates belonging to <em>Enterococcus</em> (13,76%), <em>Lactococcus</em> (18,84%) and <em>Lactobacillus</em> (67,39%) genera from fermented dairy products were used to search for their bacteriocin production and bacteriocin encoding genes were searched. LAB isolates were screened by spot-on lawn assay method against indicator microorganisms. <em>Geobacillus stearothermophilus</em> DSMZ 22, <em>Escherichia coli</

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Chigozie, Celestina Ezeagha, Ogechukwu Ogbuebuna Jacinta, Nwanyioma Anozie Amara, Chuks Oranu Emmanuel, and Henrietta Nedum Chinenye. "Determination of the Phytochemical and Antimicrobial properties of Nauclea latifolia Root Smith. (Rubiaceae)." GSC Biological and Pharmaceutical Sciences 23, no. 2 (2023): 182–88. https://doi.org/10.5281/zenodo.8041513.

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<em>Nauclea latifolia </em>is a valuable medicinal plant for treating different ailments. Infectious diseases constitute a world health concern. This work describes the phytochemical analysis and antimicrobial properties of the root of <em>N. latifolia</em>. Collected root samples were extracted via cold maceration with methanol. The methanol crude extract was fractionated into n-hexane, ethyl acetate, butanol and aqueous fractions. Phytochemical analyses and antimicrobial activity of the methanol extract/ fractions were assessed using agar well diffusion method. Four different fract

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Desjardins, Michaël, Brigitte Lefebvre, Annie-Claude Labbé, et al. "Agar Gradient Diffusion Susceptibility Testing for Neisseria gonorrhoeae: A Reliable Alternative to Agar Dilution?" Open Forum Infectious Diseases 4, suppl_1 (2017): S107. http://dx.doi.org/10.1093/ofid/ofx163.105.

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Abstract Background Antimicrobial susceptibility testing by agar dilution, the gold-standard for determination of minimal inhibitory concentration (MIC) for N. gonorrhoeae, is a labor intensive technique usually performed in reference laboratories. Agar gradient diffusion is a simpler alternative to obtain MICs. However, correlation of N. gonorrhoeae MIC values obtained by the two methods is not well established. The objective of this study is to evaluate performance of agar gradient diffusion compared with agar dilution for N. gonorrhoeae. Methods Fifty strains of N. gonorrhoeae (34 isolates

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Fagheei Aghmiyuni, Zeinab, Horieh Saderi, Parviz Owlia, and Navid Saidi. "Evaluation of the Effect of Lactobacillus acidophilus ATCC 4356 Bacteriocin against Staphylococcus aureus." BioMed Research International 2024 (March 22, 2024): 1–5. http://dx.doi.org/10.1155/2024/4119960.

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Background. Lactobacillus acidophilus is lactic acid bacteria that produce bacteriocins. Bacteriocins are antimicrobial peptides or proteins that exhibit activity against closely related bacteria. The aim of this study was to determine the effect of L. acidophilus ATCC 4356 bacteriocin against Staphylococcus aureus. Material and Methods. We used four different phenotypic methods for antimicrobial activities against two standard strains: methicillin-resistant S. aureus (MRSA) ATCC 33591 and methicillin-susceptible S. aureus (MSSA) ATCC 25923. The methods were (1) agar well diffusion, (2) overla

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Baluja, Shipra, Sumitra Chanda, Sushmita Ganguly, Swati Oza, and Kajal Nandha. "Synthesis and antimicrobial screening of some Schiff bases." GSC Biological and Pharmaceutical Sciences 9, no. 3 (2019): 099–108. https://doi.org/10.5281/zenodo.4283231.

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Some new Schiff bases were synthesized from pyrazolo aldehydes and triazoles and their structures were confirmed by IR, <sup>1</sup>H NMR, and Mass spectral data. All these synthesized compounds were tested <em>in vitro</em> for their antimicrobial potential in N, N-dimethyl formamide and dimethyl sulfoxide by agar well diffusion method. The antimicrobial strains were of Gram positive bacteria, Gram negative bacterial and fungal strains.

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Kausar, Fatima *. Mohammad Ashwaq Hruday NadhReddy Motamari Pranathi Thati Susmitha. "PHYTOCHEMICAL SCREENING &EVALUATION OF ANTIMICROBIALACTIVITY OF CHONEMORPHA FRAGRANS AGAINST HUMAN PATHOGENIC BACTERIA." Journal of Pharma Research 8, no. 5 (2019): 366–69. https://doi.org/10.5281/zenodo.3236727.

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<strong><em>ABSTRACT</em></strong>   <strong><em>T</em></strong><em>he present study aimed at evaluating the antimicrobial efficiency of ethanolic leaf extracts of Chonemorpha fragrans, a medicinal plant against four human pathogenic bacteria like Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa using agar well diffusion method and minimum inhibitory concentration and minimum bacterial concentration. Chonemorpha fragrans showed significant activity against all pathogens with its ethanolic extract which showed maximum zone of inhibition and minimum inhibit

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Azhari, A. Mohammed Nour. "Phytochemical Screening and Antibacterial Activity of Cultivated Medicinal Plants Citrus paradisi." Chemistry Research Journal 2, no. 2 (2017): 73–77. https://doi.org/10.5281/zenodo.13956279.

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Fruits are the main fruit trees grown throughout the world and are well–appreciated for their refreshing juice and health benefits. This study of <em>Citrus paradisi</em> (peels) of  hexane, acetone and methanol extracts was carried out  to show phytochemical screening and antibacterial activity. Plants and plant products are continuously being explored in medicine against the increasing number of antibiotic resistant organisms. The antibacterial activity of plant under study has been done by suing agar well diffusion method against bacteria. Phytochemical analysis showed the p

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Mkedder, Ilham, Waffa Bouali, and Hafida Hassaine. "Antibacterial Activity of Mucilage of Linum usitatissimum L. Seeds." South Asian Journal of Experimental Biology 11, no. 3 (2021): 305–10. http://dx.doi.org/10.38150/sajeb.11(3).p305-310.

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Traditional medicine has a key role in health care worldwide; the research for antimicrobial agents from plants has been a growing interest in the last few decades. Several studies indicate the presence of potent antibacterial agents in the seeds of Linum usitatissimum L. In the present study, antibacte-rial properties of mucilage extract from Linum usitatissimum L. seeds were screened against against eight Gram positive and Gram negative pathogenic bacteria using agar well diffusion method and disk diffusion method. The extract showed good results against examined bacteria and the results ob-

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Malhotra, S.P.K. and Mandal T.K. "Phytochemical screening and in vitro antibacterial activity of Moringa oleifera (Lam.) leaf extract." Archives of Agriculture and Environmental Science 3, no. 4 (2018): 367–72. https://doi.org/10.26832/24566632.2018.030406.

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The aim of the study was to investigate the phytochemical constituents and antibacterial activity of ethanolic extract of <em>Moringa oleifera </em>Lam. belonging to family Moringaceae. Distilled water and ethanol was used to extract the bioactive compounds from the leaves of <em>M. oleifera </em>to detect the phytochemical constituents and to screen its antibacterial activity. The phytochemical constituents were screened by qualitative analysis method. The phytochemical screening indicated the presence of tannins, flavonoids, glycosides, terpenoids, phenols, etc., in leaf

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Bhat, Kishore G., and Triveni Mohan Nalawade. "Antimicrobial Activity of Endodontic Medicaments and Vehicles using Agar Well Diffusion Method on Facultative and Obligate Anaerobes." International Journal of Clinical Pediatric Dentistry 9, no. 4 (2016): 335–41. http://dx.doi.org/10.5005/jp-journals-10005-1388.

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Singh, Sudha, Shamim Ahmad, Devansh Mehta, and Shamsher Alam. "Antibacterial Activity in Pyrimidine Derivatives in Lab, Using Agar Well-diffusion Method - An Update on Novel Research." Pharmaceutical Science and Technology 3, no. 2 (2019): 40. http://dx.doi.org/10.11648/j.pst.20190302.12.

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Lozano-Chiu, Mario, Page W. Nelson, Victor L. Paetznick, and John H. Rex. "Disk Diffusion Method for Determining Susceptibilities of Candida spp. to MK-0991." Journal of Clinical Microbiology 37, no. 5 (1999): 1625–27. http://dx.doi.org/10.1128/jcm.37.5.1625-1627.1999.

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We have developed an agar-based methodology for testing susceptibilities of Candida spp. to the new antifungal agent MK-0991, a glucan synthase inhibitor. Results obtained with this method correlated well with the results obtained by the National Committee for Clinical Laboratory Standards M27-A broth microdilution reference method. However, as noted with prior comparisons of broth- and agar-based systems, some isolates yielded inhibition zones which were not consistent with the MICs obtained for them. Understanding the implications of these differences will require testing in an in vivo syste

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Farias, DF, AFU Carvalho, CC Oliveira, et al. "Alternative method for quantification of alfa-amylase activity." Brazilian Journal of Biology 70, no. 2 (2010): 405–7. http://dx.doi.org/10.1590/s1519-69842010005000008.

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A modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL-1. Clear radial diffusion zones were measured after 4 hours of incubation at 20 Â°C. A linear relationship between the logarithm of enzyme activities and the area of clear zones was obtained. The method was validated by testing α-amyl

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Nalini, T. J., H. N. Ramesh Babu, N. Rajeshwari, K. Y. Prathibha, and E. Keshamma. "Assay of Antimicrobial Activity of Sida acuta (Burm. F.) by Well Diffusion Method." International Journal of Current Microbiology and Applied Sciences 11, no. 9 (2022): 183–89. http://dx.doi.org/10.20546/ijcmas.2022.1109.021.

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Sida acuta is a shrub indigenous to pantropical regions. Sida acuta belongs to the mallow family, Malvaceae. The plant is widely used for its various pharmacological properties. Therefore, in the current study antifungal activity for leaf, stem and root extracts of Sida acuta were carried out. Ethanol (50%) was used successively for extraction of active principles from the dried powdered leaves, stem and root. The antifungal screening was done with two plant pathogens viz. Claviceps purpurea MTCC 2334 and Macrophemina phaseolina MTCC 10576 as test microorganisms. In the agar-well diffusion ass

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İstanbullugil, Fatih Ramazan, and Mitat Şahin. "The Antimicrobial Effects of Kyrgyz Honey." Manas Journal of Agriculture Veterinary and Life Sciences 14, no. 1 (2024): 48–53. http://dx.doi.org/10.53518/mjavl.1472535.

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Honey is a sweet and versatile substance produced by the complex interactions between bees and flowers. Honey is not only a nutrient, but also a bee product with antibacterial properties. Kyrgyzstan's geographical location, including the Tien Shan and Altai mountains, has made it one of the most important beekeeping regions in Central Asia. The rich flora and natural environment of these regions give Kyrgyz honey unique properties. The aim of this study is the determination of the antibacterial activity of honey samples on sale in the Chuy region of Kyrgyzstan. In this context, the antimicrobi

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Iftikhar, Noreen, and Iqra Khalid. "Isolation and Characterization of Antibiotic-producing Bacteria from the Salt Range of Kallar Kahar, Pakistan." TSF Journal of Biology 1, no. 1 (2023): 67–75. http://dx.doi.org/10.69547/tsfjb.v1i1.10.

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The emergence of antibiotic resistance in pathogenic bacteria has heightened the need for new antibiotics. Streptomycetes are filamentous gram-positive bacteria that are ubiquitous and present in saline soil and produce antibiotics as secondary metabolites under stressful conditions. Naturally, Streptomyces produce over two-thirds of antibiotics that are used clinically. Saline soil was collected from Kallar Kahar, Pakistan. The soil sample was serially diluted and three dilutions were plated on Streptomyces selection media (starch-casein agar and glucose yeast malt agar) after growing the cul

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Antari, Made Juli, Ni Nyoman Puspawati, and Putu Ari Sandhi Wipradnyadewi. "AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT DARI AIR SUSU IBU (ASI) TERHADAP Listeria monocytogenes FNCC 0156." Jurnal Ilmu dan Teknologi Pangan (ITEPA) 9, no. 1 (2020): 96. http://dx.doi.org/10.24843/itepa.2020.v09.i01.p12.

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The aim of the research was to find the potency of Lactic Acid Bacteria isolated from breast milk on inhibiting Listeria monocytogenes FNCC 0156 and to find the magnitude of lactic acid bacteria from breast milk in inhibiting L. monocytogenes FNCC 0156. This reseach consists of two phase: antimicrobial activity of lactic acid bacteria from breast milk and activity of bacteriocin against L. monocytogenes FNCC 0156. Isolate used in this research was A1, A3, A6, A8, A9, B3, B7, B8, and B10b. Antimicrobial activity of lactic acid bacteria from breast milk against L. monocytogenes FNCC 0156 was per

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Samaraweera, Thumuli, Thummini Samaraweera, Nimesha Senadeera, and Chathuranga B. Ranaweera. "Evaluation of Antibacterial Activity of Endemic Jeffreycia zeylanica Plant Found in Sri Lanka." South Asian Journal of Research in Microbiology 16, no. 1 (2023): 1–9. http://dx.doi.org/10.9734/sajrm/2023/v16i1296.

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Aims: Determination of antibacterial efficacy of aqueous, methanol, dichloromethane, and hexane extracts from Jeffreycia zeylanica leaves against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Methodology: Completely ballooned leaves of J. zeylanica were gathered, air-dried, and milled into fine powder. Then macerated in all four selected solvents for about 1-2 weeks, the extracts were obtained by vacuum evaporation under reduced pressure. Antibacterial activity was performed against Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC 25922), and Pseudomonas a

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Lehtinen, Markku, Eila Pelttari, and Hannu Elo. "Antimicrobial Activity of Formylchromones: Detection by a Micro-Scale Method." Zeitschrift für Naturforschung C 66, no. 11-12 (2011): 562–70. http://dx.doi.org/10.1515/znc-2011-11-1205.

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We report the antimicrobial activity of formylchromones. These compounds are remote structural analogues of nalidixic acid and quinolone antibiotics, and their activity was investigated by a simple micro-scale method designed for the determination of minimal inhibitory concentrations (MIC) of drug candidates and antibiotics against aerobic bacteria and yeasts. Minimal bactericidal and fungicidal concentrations (MBC and MFC, respectively) were also determined in connection with the MIC determinations. The results obtained were compared with those obtained using classical agar diffusion methodol

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Mirtaghi, Seyedeh Masoumeh, Parisa Torbati Nejad, Masoumeh Mazandarani, Fasiheh Livani, and Hanieh Bagheri. "Evaluation of Antibacterial Activity of Urtica dioica L. Leaf Ethanolic Extract Using Agar Well Diffusion and Disc Diffusion Methods." Medical Laboratory Journal 10, no. 5 (2016): 15–21. http://dx.doi.org/10.18869/acadpub.mlj.10.5.15.

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Burns, Jane L., Lisa Saiman, Susan Whittier, et al. "Comparison of Agar Diffusion Methodologies for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients." Journal of Clinical Microbiology 38, no. 5 (2000): 1818–22. http://dx.doi.org/10.1128/jcm.38.5.1818-1822.2000.

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Pseudomonas aeruginosa is the most common pathogen infecting the lungs of patients with cystic fibrosis (CF). Improved antimicrobial chemotherapy has significantly increased the life expectancy of these patients. However, accurate susceptibility testing of P. aeruginosa isolates from CF sputum may be difficult because the organisms are often mucoid and slow growing. This study of 597 CF isolates of P. aeruginosa examined the correlation of disk diffusion and Etest (AB BIODISK, Solna, Sweden) results with a reference broth microdilution method. The rates of interpretive errors for 12 commonly u

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Silva, Beatriz Nunes, Olga María Bonilla-Luque, Arícia Possas, et al. "Meta-Analysis of In Vitro Antimicrobial Capacity of Extracts and Essential Oils of Syzygium aromaticum, Citrus L. and Origanum L.: Contrasting the Results of Different Antimicrobial Susceptibility Methods." Foods 12, no. 6 (2023): 1265. http://dx.doi.org/10.3390/foods12061265.

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Diffusion methods, including agar disk-diffusion and agar well-diffusion, as well as dilution methods such as broth and agar dilution, are frequently employed to evaluate the antimicrobial capacity of extracts and essential oils (EOs) derived from Origanum L., Syzygium aromaticum, and Citrus L. The results are reported as inhibition diameters (IDs) and minimum inhibitory concentrations (MICs), respectively. In order to investigate potential sources of variability in antimicrobial susceptibility testing results and to assess whether a correlation exists between ID and MIC measurements, meta-ana

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Missoun, Fatiha, Antonia Pérez de los Ríos, Víctor Ortiz-Martínez, María José Salar-García, Jesús Hernández-Fernández, and Francisco José Hernández-Fernández. "Discovering Low Toxicity Ionic Liquids for Saccharomyces cerevisiae by Using the Agar Well Diffusion Test." Processes 8, no. 9 (2020): 1163. http://dx.doi.org/10.3390/pr8091163.

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Ionic liquids (ILs) are new solvents widely used in many technologies due to their unique and advantageous physicochemical properties. In biotechnological applications, ILs can be used along with microorganisms such as Saccharomyces cerevisiae. Due to the enormous number of ILs that can be synthesized through the combination of different anions and cations, it is necessary to have an easy and quick tool for the preliminary screening of their biocompatibility for being used in biotechnological applications. In this work, the agar well diffusion test was successfully applied as a rapid method to

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Bouacha, M., S. Besnaci, and I. Boudiar. "An Overview of the Most Used Methods to Determine the In Vitro Antibacterial Activity of Honey." Acta Microbiologica Bulgarica 39, no. 1 (2023): 23–30. http://dx.doi.org/10.59393/amb23390104.

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Since ancient times, honey has been successfully used for the treatment of several infectious diseases. The objective of this review is to evaluate the different methods used in the evaluation of the antibacterial activity of honey. Several bioassays, such as disk diffusion method, well diffusion method, and broth or agar dilution method are commonly used as they do not require specific equipment. This article reviews the simple and efficient methods to evaluate the antibacterial activity of honey or any other products, such as new synthetic substances (antibiotics) or extracts of medicinal pl

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Maxime, Benedict Paguirigan Tirol, Augustine Autor Bajao Francis, Guillena Biwang Pamela, et al. "Evaluation of antibacterial property and phytochemical composition of the crude ethanolic extract from Kalingag (Cinnamomum mercadoi) barks." GSC Biological and Pharmaceutical Sciences 28, no. 1 (2024): 156–63. https://doi.org/10.5281/zenodo.13457970.

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<em>Cinnamomum mercadoi</em>, locally known as Kalingag, is a native plant of the Philippines that has limited studies despite its long use in traditional Philippine medicine due to its indigenous origin. This study examined the phytochemical composition and antibacterial activities of a crude ethanolic extract derived from Kalingag barks. The plant bark gathered from Davao Oriental was thoroughly rinsed with distilled water to eliminate any attached dirt, oven-dried for 6 hours, and then pulverized using a Wiley mill. The bark was then subjected to Soxhlet extraction, and the pooled extracts

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Maxime, Benedict Paguirigan Tirol, Augustine Autor Bajao Francis, Guillena Biwang Pamela, et al. "Evaluation of antibacterial property and phytochemical composition of the crude ethanolic extract from Kalingag (Cinnamomum mercadoi) barks." GSC Biological and Pharmaceutical Sciences 28, no. 1 (2024): 156–63. https://doi.org/10.5281/zenodo.13457970.

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<em>Cinnamomum mercadoi</em>, locally known as Kalingag, is a native plant of the Philippines that has limited studies despite its long use in traditional Philippine medicine due to its indigenous origin. This study examined the phytochemical composition and antibacterial activities of a crude ethanolic extract derived from Kalingag barks. The plant bark gathered from Davao Oriental was thoroughly rinsed with distilled water to eliminate any attached dirt, oven-dried for 6 hours, and then pulverized using a Wiley mill. The bark was then subjected to Soxhlet extraction, and the pooled extracts

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Maxime, Benedict Paguirigan Tirol, Augustine Autor Bajao Francis, Guillena Biwang Pamela, et al. "Evaluation of antibacterial property and phytochemical composition of the crude ethanolic extract from Kalingag (Cinnamomum mercadoi) barks." GSC Biological and Pharmaceutical Sciences 28, no. 1 (2024): 156–63. https://doi.org/10.5281/zenodo.13457970.

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Abstract:

<em>Cinnamomum mercadoi</em>, locally known as Kalingag, is a native plant of the Philippines that has limited studies despite its long use in traditional Philippine medicine due to its indigenous origin. This study examined the phytochemical composition and antibacterial activities of a crude ethanolic extract derived from Kalingag barks. The plant bark gathered from Davao Oriental was thoroughly rinsed with distilled water to eliminate any attached dirt, oven-dried for 6 hours, and then pulverized using a Wiley mill. The bark was then subjected to Soxhlet extraction, and the pooled extracts

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Maxime, Benedict Paguirigan Tirol, Augustine Autor Bajao Francis, Guillena Biwang Pamela, et al. "Evaluation of antibacterial property and phytochemical composition of the crude ethanolic extract from Kalingag (Cinnamomum mercadoi) barks." GSC Biological and Pharmaceutical Sciences 28, no. 1 (2024): 156–63. https://doi.org/10.5281/zenodo.13457970.

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Abstract:

<em>Cinnamomum mercadoi</em>, locally known as Kalingag, is a native plant of the Philippines that has limited studies despite its long use in traditional Philippine medicine due to its indigenous origin. This study examined the phytochemical composition and antibacterial activities of a crude ethanolic extract derived from Kalingag barks. The plant bark gathered from Davao Oriental was thoroughly rinsed with distilled water to eliminate any attached dirt, oven-dried for 6 hours, and then pulverized using a Wiley mill. The bark was then subjected to Soxhlet extraction, and the pooled extracts

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Maxime, Benedict Paguirigan Tirol, Augustine Autor Bajao Francis, Guillena Biwang Pamela, et al. "Evaluation of antibacterial property and phytochemical composition of the crude ethanolic extract from Kalingag (Cinnamomum mercadoi) barks." GSC Biological and Pharmaceutical Sciences 28, no. 1 (2024): 156–63. https://doi.org/10.5281/zenodo.13457970.

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Abstract:

<em>Cinnamomum mercadoi</em>, locally known as Kalingag, is a native plant of the Philippines that has limited studies despite its long use in traditional Philippine medicine due to its indigenous origin. This study examined the phytochemical composition and antibacterial activities of a crude ethanolic extract derived from Kalingag barks. The plant bark gathered from Davao Oriental was thoroughly rinsed with distilled water to eliminate any attached dirt, oven-dried for 6 hours, and then pulverized using a Wiley mill. The bark was then subjected to Soxhlet extraction, and the pooled extracts

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Eissa, Dalia Essam, Engy Refaat Rashed, and Mostafa Essam Eissa. "Suitability System of Microbiological Method for Nystatin Potency Determination in the Routine Analysis Using Agar Diffusion Method." SciMedicine Journal 3, no. 4 (2021): 302–15. http://dx.doi.org/10.28991/scimedj-2021-0304-2.

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Nystatin is a polyene macrolide antifungal active which is used for the treatment of candidiasis and obtained from some species of Streptomycesbacteria. The present work describes the statistical suitability analysis for regular monitoring of the agar diffusion bioassay in a simple, inexpensive and time-saving process before potency determination. A balanced (symmetrical) two-dose parallel line assay model was applied using the agar well diffusion method for quantification of Nystatin in raw material and finished medicinal dosage form. The routine inspection methodology yielded good results an

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Di Bonaventura, Giovanni, Evandro Ricci, Nicoletta Della Loggia, Giovanni Catamo, and Raffaele Piccolomini. "Evaluation of the E Test for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa Isolates from Patients with Long-Term Bladder Catheterization." Journal of Clinical Microbiology 36, no. 3 (1998): 824–26. http://dx.doi.org/10.1128/jcm.36.3.824-826.1998.

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The E test was evaluated in comparison with reference agar methods (National Committee for Clinical Laboratory Standards) for the susceptibility testing of 248 Pseudomonas aeruginosaisolates from bladder-catheterized patients against nine antibiotics. The E-test MICs correlated well with those determined by the agar dilution and disk diffusion reference methods (88 and 92.5% within 1 log2 dilution step, respectively), confirming that the E test is a reliable method for the determination of MICs of antibiotics for catheterization-associated P. aeruginosa isolates.

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Al-Mahdi, Abdulla Yahia. "Isolation and Identification of Bioactive Actinomycete Isolates from Yemen Soils." Thamar University Journal of Natural & Applied Sciences 4, no. 4 (2023): 109–19. http://dx.doi.org/10.59167/tujnas.v4i4.1297.

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A total of 50 actinomycete isolates were isolated from different locations in Yemen (Sana’a, Taiz, Ibb and Alhodida). Agar disc diffusion method that used for screening antibacterial and antifungal activities were more effective and give better results than agar well diffusion method. Antibacterial activity of isolates was more effective than antifungal activity. All five selected isolates for study were active against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Candida albicans and Microsporium canis. According to taxonomy of actinomycetes (Morphological, physiological, bioche

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Adeyemi, Adeyemo I., Omotunlese I. Vincent, and Oni M. Olujenyo. "Phytochemical screening and antifungal activity of Chromolaena odorata extracts against isolate of Phytophthora megakarya using agar-well diffusion method." Asian Journal of Medical and Biological Research 4, no. 1 (2018): 7–13. http://dx.doi.org/10.3329/ajmbr.v4i1.36815.

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In-vitro study of Chromolaena odorata extracts was determined against isolated Phytophthora megakarya for the purpose of investigating the antifungal activity of ethanolic, cold-water and warm-water extracts of the medicinal plant against Phytophthora megakarya. Their antifungal effects were assessed with agar-well diffusion methods. Phytochemical screening and minimum inhibitory concentrations of the extracts were also determined. A qualitative phytochemical analysis of the extracts showed the presence of biologically active constituents such as flavonoids, phenols, tannins, sterols, amino ac

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Vaishnav, Virendra, Debasish Sahoo, and Tanushree Chatterjee. "IN VITRO ASSESSMENT OF ANTIBACTERIAL ACTIVITY OF STEM AND ROOT OF RAUWOLFIA SERPENTINA AGAINST DIFFERENT BACTERIA." International Research Journal Of Pharmacy 12, no. 6 (2021): 87–90. http://dx.doi.org/10.7897/2230-8407.1206148.

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Medicinal Plants are the good source of natural antimicrobial agents. The main aim of present study was to evaluate the antibacterial activity of stem and root of Rauwolfia serpentina against six microorganism, Powdered stem and root of plant were extracted with acetone, chloroform and methanol and streptomycin used as positive control. The antibacterial activity of Rauwolfia serpentine was detected by using disc diffusion method and agar well diffusion method on the following bacteria- Bacillus cereus, Staphylococcus aureus, Bacillus fusiformis, Escherichia coli, Pseudomonas aeruginosa and P.

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