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Journal articles on the topic 'Aggregated stem cells'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Allen, Leah M., John Matyas, Mark Ungrin, David A. Hart, and Arindom Sen. "Serum-Free Culture of Human Mesenchymal Stem Cell Aggregates in Suspension Bioreactors for Tissue Engineering Applications." Stem Cells International 2019 (November 7, 2019): 1–18. http://dx.doi.org/10.1155/2019/4607461.

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Mesenchymal stem cells (MSCs) have the capacity to differentiate towards bone, fat, and cartilage lineages. The most widely used culture and differentiation protocols for MSCs are currently limited by their use of serum-containing media and small-scale static culture vessels. Suspension bioreactors have multiple advantages over static culture vessels (e.g., scalability, control, and mechanical forces). This study sought to compare the formation and culture of 3D aggregates of human synovial fluid MSCs within suspension bioreactors and static microwell plates. It also sought to elucidate the be

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2

Lee, D. K., C. H. Park, Y. I. Jeong, et al. "24 IMPROVEMENT OF PORCINE CLONED EMBRYONIC STEM-LIKE CELLS DERIVATION BY ZONA-MEDIATED EMBRYO AGGREGATION." Reproduction, Fertility and Development 26, no. 1 (2014): 126. http://dx.doi.org/10.1071/rdv26n1ab24.

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Porcine embryonic stem cells (ESC) have become an important model for therapeutic cloning using embryonic stem cells derived by somatic cell nuclear transfer (SCNT). However, embryo quality and blastocyst formation have been major limitations for derivation of cloned embryonic stem-like cells. In this study, we tried to overcome these problems by treating with histone deacetylase inhibitors (HDACi) and aggregating porcine embryos. A porcine embryonic fibroblast (PEF) cell line was used as the source of donor cells injected into the enucleated oocytes. First, to confirm the effect of HDACi in c

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3

Kim, J. Y., S. J. Uhm, K. S. Chung, and H. T. Lee. "188FATE OF CHIMERIC EMBRYONIC STEM CELLS RECONSTRUCTED WITH PARTHENOGENETIC MOUSE EMBRYOS." Reproduction, Fertility and Development 16, no. 2 (2004): 216. http://dx.doi.org/10.1071/rdv16n1ab188.

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Mouse parthenogenetic embryos have been known to be unable to develop to term and are absorbed from Day 11. However, Nagy et al. (1990 Development 110, 815–821) reported that the aggregated tetraploid with embryonic stem (ES) cell developed to term and survived after birth. Thus, our study investigated the developmental capacity of the aggregated ES cells with mouse parthenogenetic embryos. Oocytes obtained from superovulated female mice (BCF1) were treated with 7% ethanol and 5μgmL−1 cytochalasin B for the production of pathenotes and co-cultured with sperm (1×106mL−1) for production of ferti

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4

Wu, Fred D., Michael R. Jagir, Thomas T. Luu, and Jerry S. Powell. "Cellular Aggregates Induced from Monolayer Cultures of Human Pancreatic Cells Correct Hyperglycemia in Diabetic Mice and Demonstrate Physiological Production of Human C-Peptide." Blood 104, no. 11 (2004): 4184. http://dx.doi.org/10.1182/blood.v104.11.4184.4184.

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Abstract Diabetes is due to loss of appropriate insulin production by pancreatic islet cells, resulting in hyperglycemia and significant morbidity. We have developed cell cultures of pancreas-derived precursor cells that can be maintained for more than 20 population doublings in culture, growing as monolayers of undifferentiated cells. These monolayer cells can then be induced to differentiate into cellular aggregates that resemble islets when grown on different extracellular matrix components, including matrigel, or poly-d-lysine. The induced cellular aggregates, but not monolayer cells, corr

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Nishina, Hironobu, Chisa Katou-Ichikawa, Mizuki Kuramochi, Takeshi Izawa, Mitsuru Kuwamura, and Jyoji Yamate. "Participation of Somatic Stem Cells, Recognized by a Unique A3 Antibody, in Mucosal Epithelial Regeneration in Dextran Sulfate Sodium (DSS)–Induced Rat Colonic Lesions." Toxicologic Pathology 48, no. 4 (2020): 560–69. http://dx.doi.org/10.1177/0192623320906817.

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A3, generated as a monoclonal antibody against rat malignant fibrous histiocytoma cells, recognizes somatic stem cells in rats. We analyzed the distribution of A3-positive cells in dextran sulfate sodium (DSS)–induced colonic lesions consisting of regenerating mucosa and fibrosis. Male 6-week-old F344 rats were administered 5% DSS in drinking water for 5 to 7 days, and lesions at recovery stage were also examined. In untreated control adult colons, A3-positive cells are localized around the crypts where stem cell niche is formed. Histopathologically, in colons of DSS-administered rats, mucosal

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Lupperger, Valerio, Carsten Marr, and Prisca Chapouton. "Reoccurring neural stem cell divisions in the adult zebrafish telencephalon are sufficient for the emergence of aggregated spatiotemporal patterns." PLOS Biology 18, no. 12 (2020): e3000708. http://dx.doi.org/10.1371/journal.pbio.3000708.

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Regulation of quiescence and cell cycle entry is pivotal for the maintenance of stem cell populations. Regulatory mechanisms, however, are poorly understood. In particular, it is unclear how the activity of single stem cells is coordinated within the population or if cells divide in a purely random fashion. We addressed this issue by analyzing division events in an adult neural stem cell (NSC) population of the zebrafish telencephalon. Spatial statistics and mathematical modeling of over 80,000 NSCs in 36 brain hemispheres revealed weakly aggregated, nonrandom division patterns in space and ti

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7

Zhao, Xiaozhi, Xuefeng Qiu, Yanting Zhang, Shiwei Zhang, Xiaoping Gu, and Hongqian Guo. "Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats." Stem Cells International 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/9062638.

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It has been shown that administration of adipose derived mesenchymal stem cells (AdMSCs) enhanced structural and functional recovery of renal ischemia-reperfusion (IR) injury. Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs. The present study was designed to enhance the therapeutic effects of AdMSCs by delivering AdMSCs in a three-dimensional (3D) aggregates form. Microwell was used to produce 3D AdMSCs aggregates. In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/re

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8

Yoon, S. W., C. H. Park, S. G. Lee, et al. "225 ANTI-APOPTOTIC EFFECT OF AGGREGATION ON PREIMPLANTATION DEVELOPMENT OF BOVINE IN VITRO-FERTILIZED EMBRYOS." Reproduction, Fertility and Development 21, no. 1 (2009): 210. http://dx.doi.org/10.1071/rdv21n1ab225.

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Apoptosis occurs during embryonic development, and is related to early embryonic loss. It is important to produce high-quality blastocysts in vitro for research on the establishment of embryonic stem (ES) cells and transgenic animal production. Therefore, our objectives were to compare the anti-apoptotic effect of bovine aggregate v. nonaggregate IVF embryos and to determine whether aggregation could improve the quality of bovine embryos. The cumulus–oocyte complexes were matured for 20–22 h, and the oocytes were fertilized with cryo-preserved bovine sperm using the swim-up method. After remov

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Lee, Dong-Kyung, Chi-Hun Park, Kwang-Hwan Choi, et al. "Aggregation of cloned embryos in empty zona pellucida improves derivation efficiency of pig ES-like cells." Zygote 24, no. 6 (2016): 909–17. http://dx.doi.org/10.1017/s0967199416000241.

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SummaryThe development of embryonic stem cells (ESCs) from large animal species has become an important model for therapeutic cloning using ESCs derived by somatic cell nuclear transfer (SCNT). However, poor embryo quality and blastocyst formation have been major limitations for derivation of cloned ESCs (ntESCs). In this study, we have tried to overcome these problems by treating these cells with histone deacetylase inhibitors (HDACi) and aggregating porcine embryos. First, cloned embryos were treated with Scriptaid to confirm the effect of HDACi on cloned embryo quality. The Scriptaid-treate

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10

Layer, Paul G., Andr??e Rothermel, and Elmar Willbold. "From stem cells towards neural layers: a lesson from re-aggregated embryonic retinal cells." Neuroreport 12, no. 7 (2001): A39—A46. http://dx.doi.org/10.1097/00001756-200105250-00001.

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11

Harrison, DE, C. Lerner, PC Hoppe, GA Carlson, and D. Alling. "Large numbers of primitive stem cells are active simultaneously in aggregated embryo chimeric mice." Blood 69, no. 3 (1987): 773–77. http://dx.doi.org/10.1182/blood.v69.3.773.773.

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Abstract The possibility has been repeatedly raised that erythropoiesis results from clonal succession--the differentiation of one or a very small number of the most primitive stem cells that are sequentially activated to proliferate forming clones of differentiated cells and then eventually decline, to be replaced by new stem cell clones. We studied this possibility in chimeric mice made by combining embryos from two different strains so that they would have two distinct stem cell populations, each of which produces a different hemoglobin type (d and s). These were compared with F1 hybrids in

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Harrison, DE, C. Lerner, PC Hoppe, GA Carlson, and D. Alling. "Large numbers of primitive stem cells are active simultaneously in aggregated embryo chimeric mice." Blood 69, no. 3 (1987): 773–77. http://dx.doi.org/10.1182/blood.v69.3.773.bloodjournal693773.

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The possibility has been repeatedly raised that erythropoiesis results from clonal succession--the differentiation of one or a very small number of the most primitive stem cells that are sequentially activated to proliferate forming clones of differentiated cells and then eventually decline, to be replaced by new stem cell clones. We studied this possibility in chimeric mice made by combining embryos from two different strains so that they would have two distinct stem cell populations, each of which produces a different hemoglobin type (d and s). These were compared with F1 hybrids in which ev

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13

Yoshino, Takashi, Takahiro Suzuki, Go Nagamatsu, et al. "Generation of ovarian follicles from mouse pluripotent stem cells." Science 373, no. 6552 (2021): eabe0237. http://dx.doi.org/10.1126/science.abe0237.

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Oocytes mature in a specialized fluid-filled sac, the ovarian follicle, which provides signals needed for meiosis and germ cell growth. Methods have been developed to generate functional oocytes from pluripotent stem cell–derived primordial germ cell–like cells (PGCLCs) when placed in culture with embryonic ovarian somatic cells. In this study, we developed culture conditions to recreate the stepwise differentiation process from pluripotent cells to fetal ovarian somatic cell–like cells (FOSLCs). When FOSLCs were aggregated with PGCLCs derived from mouse embryonic stem cells, the PGCLCs entere

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14

Ahmed, Jalal, Yuya Kunisaki, Miriam Merad, and Paul S. Frenette. "Nestin+ Pericytes In The Fetal Liver Are Necessary To Maintain HSCs." Blood 122, no. 21 (2013): 583. http://dx.doi.org/10.1182/blood.v122.21.583.583.

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Abstract Although most hematopoietic stem cells (HSCs) are quiescent under homeostasis in the adult bone marrow, they are actively proliferating during development. Definitive HSCs, marked by the ability to repopulate a lethally irradiated adult mouse, are first detectable in the aorta-gonad-mesonephros region around E10.5, and then colonize the fetal liver (FL) to expand in this organ until E15 when hematopoietic activity shifts to the fetal bone marrow. The role of the microenvironment, or niche, in the regulation of HSCs in the FL, a site of physiological expansion, is unclear. Fetal liver

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15

Hao, J., J. Pareja, and N. Zaninovic. "Derivation of mouse embryonic stem cells (mESC) from individual and aggregated mouse blastomeres." Fertility and Sterility 88 (September 2007): S5. http://dx.doi.org/10.1016/j.fertnstert.2007.07.038.

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16

Lee, Byung Hoon, Jong Nam Park, Eun Ju Lee, Young Wan Moon, and Joon Ho Wang. "Therapeutic Efficacy of Spherical Aggregated Human Bone Marrow–Derived Mesenchymal Stem Cells Cultured for Osteochondral Defects of Rabbit Knee Joints." American Journal of Sports Medicine 46, no. 9 (2018): 2242–52. http://dx.doi.org/10.1177/0363546518780991.

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Background: Engraftment and longevity of transplanted cells are crucial for stem cell–based cartilage treatment. Purpose: To determine whether cultured spherical cell masses of human bone marrow–derived mesenchymal stem cells (hBM-MSCs) could improve engraftment at defect sites and to examine their corresponding effects on osteochondral regeneration. Study Design: Controlled laboratory study. Methods: A cylindrical osteochondral defect (5 mm wide × 5 mm deep) was created in trochlear grooves of rabbit knees. The single-cell type of hBM-MSCs with fibrin glue, the spherical type of hBM-MSCs with

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17

Nam, Yoojun, Yeri Alice Rim, Jennifer Lee, and Ji Hyeon Ju. "Current Therapeutic Strategies for Stem Cell-Based Cartilage Regeneration." Stem Cells International 2018 (2018): 1–20. http://dx.doi.org/10.1155/2018/8490489.

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The process of cartilage destruction in the diarthrodial joint is progressive and irreversible. This destruction is extremely difficult to manage and frustrates researchers, clinicians, and patients. Patients often take medication to control their pain. Surgery is usually performed when pain becomes uncontrollable or joint function completely fails. There is an unmet clinical need for a regenerative strategy to treat cartilage defect without surgery due to the lack of a suitable regenerative strategy. Clinicians and scientists have tried to address this using stem cells, which have a regenerat

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18

Halhouli, Tasneem, Lisa Münchhalfen, Sarkawt Hamad, et al. "Cell-Based Therapies: Ferromagnetic Versus Superparamagnetic Cell Targeting." Bioengineering 12, no. 6 (2025): 657. https://doi.org/10.3390/bioengineering12060657.

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Stem-cell-based therapies rely on the transplantation of stem cells or stem-cell-derived organotypic cells into injured tissues in order to improve or restore tissue function that has been impaired by various diseases. The potential of induced pluripotent stem cells has created many applications in the field of cell therapy, for example. Some applications, for example, those in cardiac cell therapy, suffer from low or very low efficiencies of cell engraftment. Therefore, magnetic cell targeting can be discussed as a method for capturing superparamagnetic nanoparticle-labelled cells in the tiss

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Iwasaki, Shizue, Keith H. S. Campbell, Cesare Galli, Kiyoshi Akiyama, and Setsuo Iwasaki. "Production of Live Calves Derived from Embryonic Stem-Like Cells Aggregated with Tetraploid Embryos1." Biology of Reproduction 62, no. 2 (2000): 470–75. http://dx.doi.org/10.1095/biolreprod62.2.470.

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20

Han, Fabin, Chuanguo Liu, Jin Huang, et al. "The application of patient-derived induced pluripotent stem cells for modeling and treatment of Alzheimer’s disease." Brain Science Advances 5, no. 1 (2019): 21–40. http://dx.doi.org/10.1177/2096595819896178.

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Alzheimer’s disease (AD) is the most prevalent age-related neurodegenerative disease which is mainly caused by aggregated protein plaques in degenerating neurons of the brain. These aggregated protein plaques are mainly consisting of amyloid β (Aβ) fibrils and neurofibrillary tangles (NFTs) of phosphorylated tau protein. Even though the transgenic murine models can recapitulate some of the AD phenotypes, they are not the human cell models of AD. Recent breakthrough in somatic cell reprogramming made it available to use induced pluripotent stem cells (iPSCs) for patientspecific disease modeling

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21

Lee, S. G., C. H. Park, D. H. Choi, H. Y. Son, and C. K. Lee. "282 IMPROVED QUALITY OF PORCINE BLASTOCYSTS BY AGGREGATION OF EMBRYOS AT THE 4 - 8-CELL STAGE." Reproduction, Fertility and Development 18, no. 2 (2006): 248. http://dx.doi.org/10.1071/rdv18n2ab282.

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Use of blastocysts produced in vitro would be an efficient way to generate embryonic stem (ES) cells for the production of transgenic animals and the study of developmental gene regulation. In pigs, the morphology and cell number of in vitro-produced blastocysts are inferior to these parameters in their in vivo counterparts. Therefore, establishment of ES cells from blastocysts produced in vitro might be hindered by poor embryo quality. The objective of this study was to increase the cell number of blastocysts derived by aggregating 4–8-cell stage porcine embryos produced in vitro. Cumulus–ooc

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Saitou, Mitinori, Kazushi Fujimoto, Yoshinori Doi, et al. "Occludin-deficient Embryonic Stem Cells Can Differentiate into Polarized Epithelial Cells Bearing Tight Junctions." Journal of Cell Biology 141, no. 2 (1998): 397–408. http://dx.doi.org/10.1083/jcb.141.2.397.

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Occludin is the only known integral membrane protein of tight junctions (TJs), and is now believed to be directly involved in the barrier and fence functions of TJs. Occludin-deficient embryonic stem (ES) cells were generated by targeted disruption of both alleles of the occludin gene. When these cells were subjected to suspension culture, they aggregated to form simple, and then cystic embryoid bodies (EBs) with the same time course as EB formation from wild-type ES cells. Immunofluorescence microscopy and ultrathin section electron microscopy revealed that polarized epithelial (visceral endo

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Tsai, Ching-Wen, and Tai-Horng Young. "CD44 expression trends of mesenchymal stem-derived cell, cancer cell and fibroblast spheroids on chitosan-coated surfaces." Pure and Applied Chemistry 88, no. 9 (2016): 843–52. http://dx.doi.org/10.1515/pac-2016-0405.

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AbstractCD44, a cell-surface glycoprotein, plays an important role in cell proliferation, adhesion, migration, and other biological functions, which are related with the physiological and pathologic activities of cells. Especially, CD44 is extensively expressed within adult bone marrow and has been considered as an important marker for some cancer stem cells (CSCs) in various types of tumors. Therefore, it is essential to understand the variations in CD44 expression of stem cells and cancer cells for further clinical applications. In this paper, CD44 expression was assessed on a human colon ca

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Du, Zongyun, Jihui Lao, Yuyan Jiang, et al. "Fin Cells as a Promising Seed Cell Source for Sustainable Fish Meat Cultivation." Foods 14, no. 12 (2025): 2075. https://doi.org/10.3390/foods14122075.

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Cell-cultured meat production relies on stable, proliferative seed cells, commonly sourced from muscle satellite cells (MuSCs) and adipose-derived mesenchymal stem cells (AD-MSCs). However, establishing such cell lines in fish species remains technically challenging. While pluripotent stem cells (e.g., ESCs/MSCs) offer alternatives, their differentiation efficiency and predictability are limited. Here, we developed TCCF2022, a novel caudal fin-derived cell line from Topmouth culter (Culter alburnus), which expresses pluripotency markers (AP, Oct4, Sox2, Klf4, and Nanog) and aggregated growth t

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Kanungo, Jyotshnabala, and Settara C. Chandrasekharappa. "Menin induces endodermal differentiation in aggregated P19 stem cells by modulating the retinoic acid receptors." Molecular and Cellular Biochemistry 359, no. 1-2 (2011): 95–104. http://dx.doi.org/10.1007/s11010-011-1003-2.

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Li, Xiangyun, Yuansong Yu, Wei Wei, et al. "Simple and efficient production of mice derived from embryonic stem cells aggregated with tetraploid embryos." Molecular Reproduction and Development 71, no. 2 (2005): 154–58. http://dx.doi.org/10.1002/mrd.20292.

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27

Ng, Elizabeth S., Richard P. Davis, Lisa Azzola, Edouard G. Stanley, and Andrew G. Elefanty. "Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation." Blood 106, no. 5 (2005): 1601–3. http://dx.doi.org/10.1182/blood-2005-03-0987.

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Abstract To realize the therapeutic potential of human embryonic stem cells (hESCs), it is necessary to regulate their differentiation in a uniform and reproducible manner. We have developed a method in which known numbers of hESCs in serum-free medium were aggregated by centrifugation to foster the formation of embryoid bodies (EBs) of uniform size (spin EBs). These spin EBs differentiated efficiently and synchronously, as evidenced by the sequential expression of molecular markers representing stem cells, primitive streak, and mesoderm. In the presence of hematopoietic growth factors, reprod

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Sawadkar, Prasad, Nandin Mandakhbayar, Kapil D. Patel, et al. "Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration." Journal of Tissue Engineering 12 (January 2021): 204173142110192. http://dx.doi.org/10.1177/20417314211019238.

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Current gold standard to treat soft tissue injuries caused by trauma and pathological condition are autografts and off the shelf fillers, but they have inherent weaknesses like donor site morbidity, immuno-compatibility and graft failure. To overcome these limitations, tissue-engineered polymers are seeded with stem cells to improve the potential to restore tissue function. However, their interaction with native tissue is poorly understood so far. To study these interactions and improve outcomes, we have fabricated scaffolds from natural polymers (collagen, fibrin and elastin) by custom-design

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Yoshioka, Junya, Yu Ohsugi, Toru Yoshitomi, Tomoyuki Yasukawa, Naoki Sasaki, and Keitaro Yoshimoto. "Label-Free Rapid Separation and Enrichment of Bone Marrow-Derived Mesenchymal Stem Cells from a Heterogeneous Cell Mixture Using a Dielectrophoresis Device." Sensors 18, no. 9 (2018): 3007. http://dx.doi.org/10.3390/s18093007.

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Bone marrow-derived mesenchymal stem cells (BMSCs) are an important cell resource for stem cell-based therapy, which are generally isolated and enriched by the density-gradient method based on cell size and density after collection of tissue samples. Since this method has limitations with regards to purity and repeatability, development of alternative label-free methods for BMSC separation is desired. In the present study, rapid label-free separation and enrichment of BMSCs from a heterogeneous cell mixture with bone marrow-derived promyelocytes was successfully achieved using a dielectrophore

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30

Seebauer, Lukas, Yanni Schneider, Alice Drobny, et al. "Interaction of Alpha Synuclein and Microtubule Organization Is Linked to Impaired Neuritic Integrity in Parkinson’s Patient-Derived Neuronal Cells." International Journal of Molecular Sciences 23, no. 3 (2022): 1812. http://dx.doi.org/10.3390/ijms23031812.

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Parkinson’s disease (PD) is neuropathologically characterized by the loss of dopaminergic neurons and the deposition of aggregated alpha synuclein (aSyn). Mounting evidence suggests that neuritic degeneration precedes neuronal loss in PD. A possible underlying mechanism could be the interference of aSyn with microtubule organization in the neuritic development, as implied by several studies using cell-free model systems. In this study, we investigate the impact of aSyn on microtubule organization in aSyn overexpressing H4 neuroglioma cells and midbrain dopaminergic neuronal cells (mDANs) gener

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Kolalite, M. R., A. A. Oskolski, H. G. Richter, and U. Schmitt. "BARK ANATOMY AND INTERCELLULAR CANALS IN THE STEM OF DELARBREA PARADOXA (ARALIACEAE)." IAWA Journal 24, no. 2 (2003): 139–54. http://dx.doi.org/10.1163/22941932-90000327.

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The anatomy of the primary tissues and secondary phloem in the stem of Delarbrea paradoxa Vieill. (Araliaceae) was examined with emphasis on structure and topography of secretory canals. Secretory canal systems of primary (axial canals in cortex and pith, radial canals in medullary rays) and secondary origin (axial canals in secondary phloem, radial canals in rays of secondary phloem and secondary xylem) were distinguished. Two distinct types of axial parenchyma (sheath parenchyma near axial secretory canals, and phloem parenchyma associated with conducting elements) occur in the secondary phl

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Muralidharan, Harshini, Thomas Hansen, Anja Steinle, David Schumacher, Elmar Stickeler, and Jochen Maurer. "Breast Cancer Stem Cells Upregulate IRF6 in Stromal Fibroblasts to Induce Stromagenesis." Cells 13, no. 17 (2024): 1466. http://dx.doi.org/10.3390/cells13171466.

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The microenvironment of a cancer stem cell (CSC) niche is often found in coexistence with cancer-associated fibroblasts (CAFs). Here, we show the first in-depth analysis of the interaction between primary triple-negative breast cancer stem cells (BCSCs) with fibroblasts. Using 2D co-culture models with specific seeding ratios, we identified stromal fibroblast aggregation at the BCSC cluster periphery, and, on closer observation, the aggregated fibroblasts was found to encircle BCSC clusters in nematic organization. In addition, collagen type I and fibronectin accumulation were also found at th

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Kang, H. Y., Y. K. Choi, and E. B. Jeung. "336 DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS USING SF1 INTO THE STEROID-PRODUCING CELLS." Reproduction, Fertility and Development 27, no. 1 (2015): 256. http://dx.doi.org/10.1071/rdv27n1ab336.

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Steroidogenic factor 1 (SF-1) is essential for the development and function of steroidogenic tissues. Stable incorporation of SF-1 into embryonic stem cells has been reported to prime the cells for steroidogenesis. In this study, we obtained SF1 cDNA from mRNA of murine pituitary glands and constructed the SF1-expressing vector controlled by the CMV promoter. The SF1 transgenic mouse embryonic stem cells (SF1-mES cells) were established through transfection using the nucleofector (Lonza) and selection process using G418 at 250 μg mL–1. The SF1-mES cells were aggregated in hanging drops for 2 d

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Feng, Zhonggang, Kota Sawada, Iori Ando, Riku Yoshinari, Daisuke Sato, and Tadashi Kosawada. "A Platform Integrating Biophysical and Biochemical Stimuli to Enhance Differentiation and Maturation of Cardiomyocyte Subtypes Derived from Human Induced Pluripotent Stem Cells." Journal of Cardiovascular Development and Disease 12, no. 2 (2025): 56. https://doi.org/10.3390/jcdd12020056.

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To enhance the differentiation and maturation of cardiomyocytes derived from human induced pluripotent stem cells, we developed a bioreactor system that simultaneously imposes biophysical and biochemical stimuli on these committed cardiomyocytes. The cells were cultured within biohydrogels composed of the extracellular matrix extracted from goat ventricles and purchased rat-origin collagen, which were housed in the elastic PDMS culture chambers of the bioreactor. Elastic and flexible electrodes composed of PEDOT/PSS, latex, and graphene flakes were embedded in the hydrogels and chamber walls,

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Mizukami, Takuo, Isao Hamaguchi, Kazuya Takizawa, et al. "Identification and Characterization of a Hematopoietic Stem Cell Niche in Spleen." Blood 112, no. 11 (2008): 1371. http://dx.doi.org/10.1182/blood.v112.11.1371.1371.

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Abstract In normal hematopoiesis, hematopoietic stem cells (HSCs) in bone marrow mainly produce various types of blood cells. HSCs have a function of self-renewal and are located in the osteoblastic and vascular niche, which regulates the stemness function as a stem cell microenvironment. Recent studies clearly show that several factors (N-Cadherin, b-Catenin, SDF-1a and Spp1) are involved in the maintenance of the stemness function in HSCs. However, it has also been shown that embryonic HSCs can proliferate and differentiate in extrameduller tissues like liver, spleen and placenta. In adults,

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Kawamura, K., S. Fujiwara, and Y. M. Sugino. "Budding-specific lectin induced in epithelial cells is an extracellular matrix component for stem cell aggregation in tunicates." Development 113, no. 3 (1991): 995–1005. http://dx.doi.org/10.1242/dev.113.3.995.

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We have examined immunocytochemically the expression, localization and in vivo function of a calcium-dependent and galactose-binding 14 × 10(3) Mr lectin purified from the budding tunicate, Polyandrocarpa misakiensis. Lectin granules first appeared in the inner epithelium of a double-walled bud vesicle. Soon after the bud entered the developmental phase, the granules were secreted into the mesenchymal space, where the lectin-positive extracellular matrix (ECM) developed. The lectin was also produced and secreted by granular leucocytes during budding. Hemoblasts, pluripotent stem cells in the b

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Hinge, Ashwini S., Jingyi He, Eucabeth Mose, et al. "Mitochondrial Morphology Controls Hematopoietic Stem Cell Self-Renewal and Confers Them Divisional Memory." Blood 130, Suppl_1 (2017): 633. http://dx.doi.org/10.1182/blood.v130.suppl_1.633.633.

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Abstract Hematopoietic stem cells (HSC) have extensive regenerative potential but limited self-renewal ability. The repopulation potential is gradually lost with HSC divisions and during stress hematopoiesis. Hence, we hypothesize that activation or inactivation of signaling pathways or other mechanisms, inherently occurring during HSC activation into cycle, drives their progressive functional decline. To address this, we used single cell RNA-sequencing and compared the transcriptome of HSCs [Lin-c-Kit+Sca-1+CD48-CD150+] that is expressed upon exit from quiescence and the one that is inherited

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Fennema, Eelco M., Laurent A. H. Tchang, Huipin Yuan, et al. "Ectopic bone formation by aggregated mesenchymal stem cells from bone marrow and adipose tissue: A comparative study." Journal of Tissue Engineering and Regenerative Medicine 12, no. 1 (2017): e150-e158. http://dx.doi.org/10.1002/term.2453.

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Limraksasin, Phoonsuk, Hiroko Okawa, Maolin Zhang, et al. "Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs." Stem Cells International 2020 (May 14, 2020): 1–14. http://dx.doi.org/10.1155/2020/7082679.

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Microspace culture is promising for self-organization of induced pluripotent stem cells (iPSCs). However, the optimal size of microspaces for osteogenic differentiation is unclear. We hypothesized that a specific microspace size could facilitate self-organizing iPSC differentiation to form bone-like tissue in vitro. The objectives of this study were to investigate such effects of microspace size and to evaluate bone regeneration upon transplantation of the resulting osteogenic constructs. Dissociated mouse gingival fibroblast-derived iPSCs were plated in ultra-low-attachment microspace culture

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Mohammadi Ghahhari, Nastaran, Faezeh Maghsood, Saeed Jahandideh, et al. "Secretome of Aggregated Embryonic Stem Cell-Derived Mesenchymal Stem Cell Modulates the Release of Inflammatory Factors in Lipopolysaccharide-Induced Peripheral Blood Mononuclear Cells." Iranian Biomedical Journal 22, no. 4 (2018): 237–45. http://dx.doi.org/10.29252/ibj.22.4.237.

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Laksmitawati, Dian R., Jeanne A. Pawitan, Mohamad Sadikin, Caroline T. Sardjono та Ahmad R. Utomo. "IFNγ modulates human immunoglobulin receptor expression in lipoaspirate-derived mesenchymal stem cells". Medical Journal of Indonesia 23, № 3 (2014): 127–32. http://dx.doi.org/10.13181/mji.v23i3.923.

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Background: Mesenchymal stem cell (MSC) has been reported to have immunomodulator capacity against autoimmune diseases and to prevent allogenic tissue rejection. Many studies revealed that MSC’s inhibit T cell proliferation and induce immunosuppressive condition through the production of prostaglandins, and interleukin-10. In addition, MSC was reported to reduce circulating autoantibody in autoimmune patients following MSC transfusion. So far, there has been no report stating the presence of Fc receptors (receptors for immunoglobulin) on MSCs. The aim of this study was to reveal the expression

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Zeng, Xin, Ya-nan Liu, Zhen Li, et al. "Induced differentiation of adipose-derived stem cells enhance secretion of neurotrophic factors." Investigación Clínica 64, no. 3 (2023): 267–80. http://dx.doi.org/10.54817/ic.v64n3a01.

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Adipose-derived stem cells (ADSCs) could be ideal seed cells for repairing nerve injury as they have the potential for multidirectional dif-ferentiation. However, it is still unclear whether the undifferentiated or the differentiated ADSCs have priorities in promoting axonal regeneration and my-elin formation. In this study, the primary ADSCs from rats were cultured and differentiated. The morphology, differentiation potential, and secretion of neu-rotrophic factors of ADSCs were compared before and after induction. Undiffer-entiated ADSCs (uADSCs) were aggregated into bundles containing retic

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Park, J. K., H. S. Kim, K. J. Uh, et al. "215 COMPARATIVE ANALYSIS OF PORCINE PLURIPOTENT CELL LINES DERIVED FROM SOMATIC CELLS AND VARIOUS EMBRYONIC ORIGINS." Reproduction, Fertility and Development 24, no. 1 (2012): 220. http://dx.doi.org/10.1071/rdv24n1ab215.

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Since pluripotent cells were first derived from the inner cell mass (ICM) of mouse blastocysts, tremendous efforts have been made to establish embryonic stem cell (ESC) lines in several domestic species including the pig; however, authentic porcine ESCs have not yet been established. It has proven difficult to derive pluripotent cells of naïve state that represents full pluripotency, due to the frequent occurrence of spontaneous differentiation into an EpiSC-like state during culture in pigs. We have been able to derive EpiSC-like porcine embryonic stem cell (pESC) lines of a differentiated no

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Amano, T., M. Gertsenstein, A. Nagy, H. Kurihara, and H. Suzuki. "Nuclear transfer reprogramming does not improve the low developmental potency of embryonic stem cells induced by long-term culture." Reproduction 132, no. 2 (2006): 257–63. http://dx.doi.org/10.1530/rep.1.01117.

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Epigenetic states of embryonic stem (ES) cells are easily altered by long-term cultivation and lose their developmental potential. To rescue this reduced developmental capacity, nuclear transfer (NT) of ES cells was carried out, and original ES and ES cells from cloned blastocysts (ntES) cells established after NT were compared with in vitro differentiation ability and developmental potential by embryoid body formation and tetraploid aggregation respectively. In the establishment of ntES cell lines, the oocytes fused with the ES cell were activated, and further cultured to cloned blastocysts.

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Wang, Meicong, Lie Ma, Bo Li, and Changyou Gao. "Modification of Polycaprolactone Microspheres-Aggregated Scaffolds with Galactosylated Chitosan for Modulating the Differentiation of Bone Mesenchymal Stem Cells." Journal of Biomaterials and Tissue Engineering 4, no. 8 (2014): 657–62. http://dx.doi.org/10.1166/jbt.2014.1216.

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Borghese, Cinzia, Naike Casagrande, Giuseppe Corona, and Donatella Aldinucci. "Adipose-Derived Stem Cells Primed with Paclitaxel Inhibit Ovarian Cancer Spheroid Growth and Overcome Paclitaxel Resistance." Pharmaceutics 12, no. 5 (2020): 401. http://dx.doi.org/10.3390/pharmaceutics12050401.

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Adipose-derived stem cells (ADSCs) primed with paclitaxel (PTX) are now hypothesized to represent a potential Trojan horse to vehicle and deliver PTX into tumors. We analyzed the anticancer activity of PTX released by ADSCs primed with PTX (PTX-ADSCs) (~20 ng/mL) in a panel of ovarian cancer (OvCa) cells sensitive or resistant to PTX. We used two (2D) and three dimensional (3D) in vitro models (multicellular tumor spheroids, MCTSs, and heterospheroids) to mimic tumor growth in ascites. The coculture of OvCa cells with PTX-ADSCs inhibited cell viability in 2D models and in 3D heterospheroids (S

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Kim, H. M., J. K. Park, S. G. Lee, et al. "281 ATTEMPTS FOR ESTABLISHMENT OF PORCINE EMBRYONIC STEM-LIKE CELLS DERIVED FROM IN VITRO-PRODUCED BLASTOCYSTS." Reproduction, Fertility and Development 21, no. 1 (2009): 237. http://dx.doi.org/10.1071/rdv21n1ab281.

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The porcine embryonic stem (ES) cells could be a useful tool for the production of transgenic animals and the study of developmental gene regulation. Even though the efficiency of establishment of ES cells from in vivo blastocysts is relatively high, especially in mice, it is difficult and expensive to obtain in vivo embryos in domestic animals. Recent development of techniques in the production of embryos in vitro could be a useful source for the establishment of ES cells. However, the morphology and cell quality of in vitro-produced embryos are inferior to those of their in vivo counterparts

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Hyun, Young Youl, In Ok Kim, Mi Hyung Kim, et al. "Adipose-Derived Stem Cells Improve Renal Function in a Mouse Model of IgA Nephropathy." Cell Transplantation 21, no. 11 (2012): 2425–39. http://dx.doi.org/10.3727/096368912x639008.

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T-cell dysregulation plays an important role in the pathogenesis of immunoglobulin A nephropathy (IgAN). Adipose-derived stem cells (ASCs) have been reported to be able to prevent tissue damage through immune-modulating effects. To evaluate the effects of ASCs in high IgA ddY (HIGA) mice, ASCs were isolated from HIGA mice with different stages of IgAN before and after disease onset. ASCs were injected at a dose of 5 × 106 cells/kg body weight through the tail vein every 2 weeks for 3 months. Although the administered ASCs were rarely detected in the glomeruli, 24-h proteinuria was markedly dec

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Hara, Yaiko . "Morphological Study on Development of Vascular Endothelium in Chick Embryo." JOURNAL OF MULTIDISCIPLINARY DENTAL RESEARCH 8, no. 2 (2022): 57–60. http://dx.doi.org/10.38138/jmdr/v8i2.22.29.

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The vascular endothelium and hematopoietic stem cells are believed to derive from common progenitor cells called hemangioblasts. However, morphological study on the development of vascular endothelial cell has been scarce. The present study is intended to provide more morphological explanation of the differentiation process of the hemangioblast. We performed histological observation, using 10 four-day-old chick embryos. After fixation with 4% concentration paraformaldehyde, hematoxylin eosin staining, toluidine blue staining and immunostaining were performed in that order for observation of mo

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Zhao, Kaixuan, Yue Shi, Jia Yu, et al. "Intracameral Microimaging of Maturation of Human iPSC Derivatives into Islet Endocrine Cells." Cell Transplantation 31 (January 1, 2022): 096368972110665. http://dx.doi.org/10.1177/09636897211066508.

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We exploited the anterior chamber of the eye (ACE) of immunodeficient mice as an ectopic site for both transplantation and microimaging of engineered surrogate islets from human induced pluripotent stem cells (hiPSC-SIs). These islets contained a majority of insulin-expressing cells, positive or negative for PDX1 and NKX6.1, and a minority of glucagon- or somatostatin-positive cells. Single, non-aggregated hiPSC-SIs were satisfactorily engrafted onto the iris. They underwent gradual vascularization and progressively increased their light scattering signals, reflecting the abundance of zinc-ins

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