Dissertations / Theses: 'Aging DNA DNA fingerprinting'

1

Roring, Solvig Mary Margaret. "DNA fingerprinting of Mycobacterium bovis." Thesis, Queen's University Belfast, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287426.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Zhu, Jiahui. "DNA fingerprinting in Oryza sativa L." Thesis, University of East Anglia, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338095.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

何兆康 and Siu-hong Ho. "Isolation and characterization of Panax Ginseng repetitive DNA sequences for DNA fingerprinting." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31215282.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Ho, Siu-hong. "Isolation and characterization of Panax Ginseng repetitive DNA sequences for DNA fingerprinting /." Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19737816.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Kennedy, Bobbie-Jo. "DNA fingerprinting of Native American skeletal remains." Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/958779.

Full text

Abstract:

The purpose of this project was to determine if the human skeletal remains of two distinct Native American cemeteries, found in close geographic proximity, represent the same population. These archaeological sites are similar in location and artifacts. Burial practices, however, vary between the sites. These differences may represent class distinction or a difference in the times the cemeteries were used. Radiocarbon techniques have given dates of AD 230±300 and AD 635±105 for these two sites. Several methods of DNA isolation were compared for their ability to yield PCR amplifiable DNA. DNA isolation using a combination of CTAB and phenol/chloroform/isoamyl alcohol (24:24:1) provided the best results and yielded amplifiable DNA form two individuals, Hn I (8F-410) and Hn 10 ( 27F-8-14 b). Purification of the DNA by extraction from low melting agarose gel was required prior to PCR, and PCR conditions were optimized to maximize the DNA yields. Regions of the mitochondrial DNA (mtDNA) genome of isolated DNA were amplified by PCR using primers which are specific for the HincII region of the mtDNA genome. Inability of restriction enzyme HincII to digest the amplified DNA of these two individuals suggested that they belong to the Native American mtDNA lineage C characterized by the loss of this restriction site.
Department of Anthropology

APA, Harvard, Vancouver, ISO, and other styles

6

Meng, Anming. "DNA fingerprinting and minisatellite variation of swans." Thesis, University of Nottingham, 1990. http://eprints.nottingham.ac.uk/13889/.

Full text

Abstract:

Genetic variation in natural populations of four species of swans (Cygnus bewickii, Cygnus olor, Cygnus buccinator and Cygnus cygnus) has been investigated by examining minisatellite loci using human DNA fingerprinting probes pSPT19.6 and pSPT18.15. It has been found that swan minisatellites are highly variable. However, the degree of variation depends on the population structure and species. Bewick's Swans at Slimbridge have the highest degree of minisatellite variation, Whooper Swans at Caerlaverock come second, and then Mute Swans, and Trumpeter Swans in Montana. Comparative study of DNA fingerprints among populations and among species suggested that swan minisatellites are subject to specific as well as population differentiation, although the function of minisatellites remains an unsolved mystery. Hypervariable minisatellites of swans that are detected by DNA fingerprinting are stably inherited as codominant markers. DNA fingerprinting has been used to study mating behaviour of Mute and Whooper Swans in the wild The results showed that the Whooper swans were almost strictly monogamous and Mute Swans exhibited an adaptable reproductive system. A genomic library from Cygnus olor was constructed and dozens of minisatellites were isolated. Most of the cloned swan minisatellites were variable, some showed specific variation, and one (pcoMS6.1) detected RFLPs in PstI digests of Trumpeter Swans.

APA, Harvard, Vancouver, ISO, and other styles

7

Carter, Royston Edwin. "Development adaptations & applications of DNA fingerprinting." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336944.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Derksen, Linda Anne. "Agency and structure in the history of DNA profiling : the stabilization and standardization of a new technology /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC IP addresses, 2003. http://wwwlib.umi.com/cr/ucsd/fullcit?p3083460.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Choy, Yan-tsun. "Statistical evaluation of mixed DNA stains." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42664287.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Groft, Donald G. "DNA fingerprinting of Alberta bull trout (Salvelinus confluentus) populations." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ38445.pdf.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Groft, Donald G., and University of Lethbridge Faculty of Arts and Science. "DNA fingerprinting of Alberta bull trout (Salvelinus confluentus) populations." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1997, 1997. http://hdl.handle.net/10133/80.

Full text

Abstract:

Bull trout (Salvelinus confluentus) populations from Alberta river drainage systems were compared using molecular techniques. Restriction fragment length polymorphisms (RFLP's) within the NDI and ND5/6 regions of the mitochondrial genome were observed. In addition, randomly amplified polymorphic DNA profiles (RAPD's) from total genomic DNA extracts were compared. Interdrainage comparisons using mtDNA revealed significant population heterogeneity among Alberta bull trout. Percent sequence divergence in mtDNA ranged from 0.14% to 0.92%. Most fish in each population were composed of a small number of common haplotypes, and the remaining fish displayed rare or locally unique haplotypes. RAPD profiles were used to calculate genetic distance values for Alberta, Canada and Montana, U.S.A. populations. Both Nei and Cavalli-Sforza distance values were used to generate neighbor-joining, FITCH and KITSCH distance trees. Two genetically distinct groups of bull trout were revealed by the RAPD analysis and the possiblity that post-glacial bull trout populations are derived from two separate refugia is suggested.
xvii, 161 leaves : ill. ; 28 cm.

APA, Harvard, Vancouver, ISO, and other styles

12

Burr, Mark Daniel 1949. "An evaluation of DNA fingerprinting methods for subtyping Salmonella." Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/290630.

Full text

Abstract:

The use of DNA typing and fingerprinting methods to identify and discriminate strains of bacteria, including Salmonella, has increased dramatically in recent years. Traditional typing methods, including serotyping and phage typing, have often not adequately discriminated strains, nor have they always identified virulent or antibiotic resistant strains. In a literature review, DNA-based methods, including plasmid analysis, restriction fragment length polymorphism (RFLP) analysis, and polymerase chain reaction (PCR) fingerprinting methods were evaluated. Plasmid analysis, including plasmid profiles and plasmid fingerprints have been shown to be useful primarily in short-term investigations of disease outbreak. However, plasmid profiles or possession of individual plasmids have generally not been good indicators of cell phenotypes overall. RFLP fingerprinting of Salmonella utilizing probes from ribosomal DNA, insertion sequence IS200, or random sequences has been reported. Ribotypes detected with ribosomal probes have generally been shared among different serotypes, whereas IS200 profiles have tended to be more serotype-specific. AP PCR and rep-PCR primers have been shown to discriminate Salmonella isolates, but fingerprints have been more difficult to reproduce and interpret than RFLP fingerprints. Several authors have reported bands of varying intensities, and some faint bands have not been reproducible. Improved methods of resolving and detecting PCR products are necessary. In a laboratory study, 85 environmental Salmonella isolates belonging to 22 serotypes were fingerprinted by 16S RFLP ribotyping, by rep-PCR, using ERIC (enterobacterial repetitive intergenic consensus) primers, and by AP PCR. Ribotypes were shared by isolates from different serotypes. ERIC PCR and one AP PCR primer produced fingerprints that discriminated among the different isolates, but did not identify serotypes. Another AP PCR primer produced simple patterns that neither discriminated isolates, nor identified serotypes. In a second related laboratory study, computer-assisted matching of AP PCR fingerprints of several known isolates was evaluated. Aliquots of the PCR reaction were run in the same and different gels, and the fingerprints bands were scored by two technicians on a presence-absence basis, and matched by creating dendrograms. Although replicate fingerprints of an isolate appeared reproducible, they were not always scored identically. Thus, the computer was not always able to correctly match fingerprints.

APA, Harvard, Vancouver, ISO, and other styles

13

Bischof, Laura Louise. "DNA fingerprinting analysis of captive Asian elephants, Elephas maximas." PDXScholar, 1990. https://pdxscholar.library.pdx.edu/open_access_etds/3966.

Full text

Abstract:

This thesis examined the effectiveness of DNA fingerprinting analysis for paternity ascertainment and the establishment of relatedness of captive Asian elephants (Elephas maximas). Eighteen Asian elephants from three North .American zoos were examined. Thirteen of these elephants were wild caught. Relationships between these elephants and the remaining elephants born in captivity were known.

APA, Harvard, Vancouver, ISO, and other styles

14

Choy, Yan-tsun, and 蔡恩浚. "Statistical evaluation of mixed DNA stains." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B42664287.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Van, Ijperen Antonia Cornelia. "Fingerprinting and characterisation of Escherichia coli isolates using DNA arrays." Thesis, University of Greenwich, 2005. http://gala.gre.ac.uk/6329/.

Full text

Abstract:

Two commercially available DNA whole genome Escherichia coli K12 arrays were compared to identify a subset of markers for typing. The arrays were identical in probe composition but different in substrate (membrane and glass slide arrays) and probe preparation (radio- and fluorescent-labelled). Labelled genomic E. coli DNA from five strains of the E. coli reference (ECOR) collection (ATCT35320 - ATCX35324) and E. coli K12 were hybridised against these arrays. A group of 1240 putative markers was identified on the membrane arrays and 649 were found on the glass slide arrays. Only a small proportion of these sequences (8%) was found through both platforms. Variability in the hybridisation signals from duplicate experiments made it difficult to identify useful markers. In order to investigate whether this technology could be used for characterising or typing E. coli strains, an array for the detection of 29 pathogenicity markers in E. coli strains was produced. This array was used with eight reference strains, including different pathotypes, 72 strains from the ECOR collection, and 49 clinical isolates. A wide range of E. coli pathogenicity markers was detected. The pathogenicity markers that were most common include chuA and iucC, which are both involved in iron metabolism. Additionally, the clinical isolates were grouped into clusters different from groupings based on biochemical tests. This demonstrates that the use of pathogenicity array typing can complement diagnostic tests on clinical E. coli isolates. An extended, second-generation, pathogenicity marker array containing 75 probes was made. The extended array successfully distinguished between ten closely related isolates from an outbreak of urinary tract infections, while previous tests were unable to do so. This array has the potential for providing a rapid and novel means of characterising pathogenic isolates.

APA, Harvard, Vancouver, ISO, and other styles

16

Appuhamy, Sarah. "DNA fingerprinting of Haemophilus somnus, Histophilus ovis and Actinobacillus seminis." Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266458.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Chan, May-ngor. "Isolation and characterization of repetitive DNA sequences and their use in DNA fingerprinting and the population genetics of Perna viridis (L.) (Bivalvia : Mytilidae) /." Hong Kong : University of Hong Kong, 1997. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19470423.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Kremer, Stefanie Lee. "The use of miniSTRS and mitochondrial DNA to identify handlers of pipe bombs." Diss., Connect to online resource - MSU authorized users, 2008.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

19

Morrison, Donald. "Evaluation of DNA typing methods for Enterococcus faecium." Thesis, University of London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312043.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Stacey, Glyn Nigel. "A study of the molecular genetic stability and diversity of animal cells." Thesis, Open University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294828.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Armour, John Anthony. "The isolation and characterization of human minisatellite loci." Thesis, University of Leicester, 1990. http://hdl.handle.net/2381/35423.

Full text

Abstract:

Hypervariable minisatellite regions of human DNA are of considerable interest, not only as highly informative genetic systems, but also as intermediately sized regions of tandem repetition. Methods for the isolation of minisatellite loci from the human genome have been investigated, and 23 new hypervariable loci cloned from an ordered array Charomid library. This method not only allows very efficient isolation of human minisatellites, but can also be used to observe the degree of overlap between multi-locus DNA fingerprinting probes. The 23 new loci have a mean heterozygosity level of 71%, and have been characterized and mapped in the genome. The genomic disposition of human minisatellites has been analysed by investigation of cloned examples. The minisatellites studied show a strong tendency to cluster near the ends of chromosomes, and sequence analysis demonstrates a significant excess of dispersed repeat elements in the DNA flanking human minisatellites. Minisatellite variant repeat (MVR) mapping has also been used to investigate the internal structure of minisatellite alleles. Somatic allele length mutation events have been demonstrated in DNA from colorectal adenocarcinomas, and the mutations observed show many features of general similarity to germline mutation events. A series of human breast tumours has been screened for somatic change, using both multi-locus DNA fingerprinting probes and single-locus minisatellite probes. Somatic change in breast cancers is much less frequent than in colorectal tumours, but some allele losses and mutations have been defined, including a highly unusual mutation, which may be the result of a minisatellite transposition event. Finally, evolution at minisatellite loci has been studied, both by examination of allelic states in current human populations, as well as comparison with non-human primates.

APA, Harvard, Vancouver, ISO, and other styles

22

Dean, Kristina. "Degradability of both a physical latent fingerprint and its associated extracted DNA." [Cedar City, Utah] : Southern Utah University, 2009. http://unicorn.li.suu.edu/ScholarArchive/ForensicScience/DeanKristina.pdf.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Choudhury, Arpita. "The use of microsatellite DNA fingerprinting for aquaculture and fisheries science /." View online ; access limited to URI, 2005. http://0-wwwlib.umi.com.helin.uri.edu/dissertations/dlnow/3186898.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Goll, Melanie. "Nachweis und DNA-Fingerprinting von Escherichia-coli-O157-Stämmen bei Pferden." Wetteberg : VVB Laufersweiler, 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=976072742.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Ashworth, David. "The application of DNA fingerprinting to the conservation of threatened species." Thesis, University of Nottingham, 1992. http://eprints.nottingham.ac.uk/29183/.

Full text

Abstract:

The human polycore minisatellite probes 33.6 and 33.15 developed by Prof. Alec Jeffreys and colleagues have been shown to detect hypervariable minisatellites in many taxonomically dispersed species. The mRNA derivatives of these two probes, pSPT19.6 and pSPT18.15, have here been used to probe the genomes of four species currently maintained in captivity. The wild populations of these species, Rothschild's mynah, the Rodrigues fruit bat, the British Merlin and the New Zealand falcon, are threatened with extinction to varying degrees. By using the technique of DNA fingerprinting, it has been possible to assess the levels of minisatellite variation remaining in these stocks, to confirm or refute the parent/offspring allocations made within, and in the case of Rothschild's mynah, to demonstrate that at least two of the founders of the stock were closely related. In addition, it has been possible to show that there is a significant positive relationship between the similarity coefficient calculated between two adults and the inbreeding coefficient calculated for their offspring.

APA, Harvard, Vancouver, ISO, and other styles

26

陳美娥 and May-ngor Chan. "Isolation and characterization of repetitive DNA sequences and their use in DNA fingerprinting and the population genetics of Perna viridis(L.) (Bivalvia : Mytilidae)." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1997. http://hub.hku.hk/bib/B31214964.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Woodburn, Mary Alice. "Random amplified polymorphic DNA (RAPD) analysis of Bacillus sphaericus." Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-07102009-040429/.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Vaziri, Homayoun. "Telomeres, DNA damage signaling molecules and cellular aging." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0020/NQ45836.pdf.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Park, Peter Unnam 1971. "DNA repair and genomic instability in yeast aging." Thesis, Massachusetts Institute of Technology, 2002. http://hdl.handle.net/1721.1/8389.

Full text

Abstract:

Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 2002.
Includes bibliographical references.
Aging is a universal process that occurs in eukaryotic organisms. Many features of aging, including the genetic pathways involved in aging, appear to be evolutionarily conserved. Extrachromosomal rDNA circles (ERCs) have been identified to be a cause of replicative aging in budding yeast S. cerevisiae. Genomic instability can cause ERCs to excise from chromosomal rDNA arrays, containing 35S and 5S rRNA genes. At each cell division, ERCs replicate via an origin of replication present in each rDNA repeat, accumulate asymmetrically in the mother cells due to their segregation bias, and ultimately cause nucleolar fragmentation and senescence. Introduction of an ERC into young cells shortens life span and accelerates the onset of age-associated sterility. ERCs are excised from the rDNA locus by homologous recombination. rad52 mutant cells, defective in DNA repair through homologous recombination, do not accumulate ERCs with age; likewise, mutations in other genes of the RAD52 class that have varying effects on homologous recombination have corresponding effects on ERC formation. rad52 mutation leads to a progressive delocalization of a silencing and DNA repair protein Sir3p from telomeres to other nuclear sites with age and, surprisingly, shortens life span despite the absence of ERCs. Spontaneous DNA damage, perhaps double-strand breaks (DSBs), are likely the cause of lethality in mutants of the RAD52 class and may be an initial step in aging in wild-type cells. Replication fork pausing in E. coli can cause DSBs. Consistently, afobl mutation, which abolishes unidirectional replication fork barrier (RFB) at the rDNA, reduces rDNA recombination, decreases ERC accumulation with age, and thus extends life span.
(cont.) Therefore, reduction of ERC formation infobl cells is likely the consequence of an absence of DSBs caused by replication fork pausing at RFB, again suggesting that DSBs are the initial step in yeast aging. Foblp is also required for transcription enhancement from an ectopic RNA polymerase I (Pol I) promoter outside of the rDNA, but not at rDNA. The role of Foblp in this enhancement may be to facilitate interactions between rDNA repeats and the ectopic promoter, thus allowing recruitment of Pol I transcriptional machinery to the ectopic promoter. Consistent with this notion, Foblp influences the structural dynamics at rDNA. Foblp antagonizes Sir2p-dependent rDNA silencing and thus affects rDNA chromatin structure. fobl mutation partially suppresses the aberrant rDNA structure in topoisomerase I-deficient (topl) cells, likely by relieving torsional stress created during rDNA replication. In addition, afobl mutation was able to rescue the synthetic lethality in topl trf4 double mutant cells, due to the failure of mitotic condensation at rDNA. fobl mutation complemented several defects caused by trf4 mutation, suggesting that Trf4p, DNA polymerase [sigma], may have a specialized function at rDNA. FOB1 and likely RFB may play a direct role in the macromolecular rDNA structure that influences chromosome transmission and indirectly affects aging in yeast.
by Peter Unnam Park.
Ph.D.

APA, Harvard, Vancouver, ISO, and other styles

30

Noyes, H. A. "PCR based approaches to the identification and classification of Leishmania." Thesis, University of Liverpool, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309913.

Full text

Abstract:

Random amplified polymorphic DNA (RAPD) was tested for the identification and classification of Leishmania. RAPD was found to be useful for the identification of species of L. (Leishmania) and L. (Yiannia) and for the classification of L. (Yiannia) species. The polymerase chain reaction (PCR) was tested for the identification of Leishmania from mammals and lizards, using both published primers and new primers which amplify kinetoplast minicircle DNA. The size of the PCR product was found to be useful for discriminating between some sympatric pairs of species such as L. braziliensis and L. mexicana. Isotopically labelled probes prepared from the variable region of the kinetoplast minicircle were tested for specificity for the identification of New and Old World species of Leishmania. The specificity was dependent on the concentration of target DNA and was manipulated to investigate relationships between Leishmania species. Restriction digests of kinetoplast DNA (schizodemes) prepared by PCR and by centrifugation through 20% sucrose were compared for the identification of strains of L. infantum and L. chagasi. Twenty three strains of L. chagasi from cases of visceral and cutaneous leishmaniasis in Honduras were examined by RAPD, schizodemes, differential display, isoenzyrnes, RFLPs and PFGE to discover whether genetic differences existed between parasites causing the two different pathologies. The parasites were found to be unusually homogeneous and no differences were found which correlated with pathology by any of these methods. Restriction digests of PCR amplified small subunit ribosomal DNA (SSU rDNA) (ribodemes) were tested to find markers specific for the genus Leishmania. A classification of the Leishmania based on the restriction fragments indicated that L. hertigi and L. herreri were more closely related to Endotrypanum than to Leishmania, and that the lizard Leishmania could not be placed in separate genus from the Leishmania. Ribodemes were used to identify two strains of parasites supplied by colleagues in Central America that could not be identified by existing methods for the identification of Leishmania. One of these strains appeared to be identical to a C. luciliae reference strain. The other strain produced a fingerprint unlike any of the available reference strains. A variable region of the SSU rRNA gene was identified that was suitable for classifying trypanosomatids and the sequence of this region was used to classify the strain that could not be identified by fingerprinting.

APA, Harvard, Vancouver, ISO, and other styles

31

Soleimani, Abdulvahab D. "Genetic diversity estimates and DNA fingerprinting of Canadian cultivars of durum wheat." Thesis, University of Ottawa (Canada), 2001. http://hdl.handle.net/10393/6192.

Full text

Abstract:

Estimates of genetic diversity present in gene pools have important implications for breeders and germplasm curators. They constitute the raw material for plant improvement and can provide protection against genetic vulnerability to biotic and abiotic stresses. The purpose of this thesis was to derive Pedigree and Amplified Restricted Fragment Polymorphism (AFLP)-based Genetic Diversity Estimates (GDEPED and GDEAFLP, respectively) among all currently registered 13 Canadian durum wheat cultivars in order to test the hypothesis that the actual level of genetic variation at the DNA level is lower than what is measured from pedigree data. Two objectives were set in this thesis. The first objective was to estimate the genetic diversity level among cultivars using two independent methods namely AFLP and pedigree. The second objective of this thesis was to use cultivar-specific AFLP markers for identification of cultivars and transform these markers into sequence tagged site (STS) markers for routine identification of durum wheat cultivars. (Abstract shortened by UMI.)

APA, Harvard, Vancouver, ISO, and other styles

32

Li, Suiyang. "Establishment of an inbreeding index in Holstein dairy cattle using DNA fingerprinting." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=69763.

Full text

Abstract:

In order to establish a method of assessing the degree of inbreeding within herds of cattle, we constructed a calibration index relating kinship and the degree of DNA band sharing in DNA fingerprints. Firstly, chickens were used as a model system to test the possibility of using microsatellite DNA as a probe for DNA fingerprinting in inbreeding analysis. Six genetic groups of chickens with estimated coefficients of inbreeding ranging from 0.026 to $>$ 0.98 (pedigree analysis) were fingerprinted using the minisatellite probe derived from M13 and the microsatellite probe (CAC)$ sb5$. The degree of band sharing using either probe increased in concert with the known amount of inbreeding and was described by the equation Y = 0.56X ($ pm$0.06) + 0.42 ($ pm$0.03); r = 0.998. Since in-gel hybridization using the microsatellite probes was faster and less labour intensive than using the minisatellite probe, it was used in the subsequent studies. Pedigree analysis in Holstein dairy cattle allowed for the empirical calibration of the association of band sharing with the coefficient of relatedness, (r), defined as the expected proportion of genes in 2 individuals that are identical by descent (i.e. for monozygous twins r = 1; for first order relatives r = 0.5; for half sibs r = 0.25 etc.). The average band sharing between pairs (6 pairs at each r value) of individuals within each class formed the basis for calibration. DNA was digested using RsaI. The relationship between band sharing and relatedness was well represented by a linear approximation Y = 0.51X ($ pm$0.09) + 0.50 ($ pm$0.04); r = 0.992. Using this calibration curve, random samples of animals within herds can be tested to establish the herd variability and to minimize inbreeding.

APA, Harvard, Vancouver, ISO, and other styles

33

Saxon, Herbert. "The molecular biology of orchids : transformation by Agrobacterium Tumefaciens and DNA fingerprinting." Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/941575.

Full text

Abstract:

The work reported here was done at the Wheeler Orchid Collection and Species Bank and the Department of Biology at Ball State University. We have developed a research teaching program with two applied research goals: genetically transforming and DNA fingerprinting orchid tissue. As part of their molecular biology education, students have investigated the genetic transformation of orchids for mitigating viral symptoms and the identification of unknown orchids by DNA fingerprinting. In a second application of the technology, DNA fingerprinting has been used to determine evolutionary relationships and to quantify genetic diversity among orchids.This dissertation details the background and need for this project and the research that was done to start it. As the early work has, developed and students have added their contributions, the data have developed into two papers formatted for submission to scientific journals. They are included as results.The first is a project designed to insert exognenous DNA into orchid tissue. The soil microbe Agrobacterium tumefaciens causes crown-gall tumors to develop in its plant hosts by inserting DNA into their cells which then controls the biosynthesis of development-controlling hormones. A. tumefaciens which has been disarmed has been routinely used to bioengineer dicotyledonous plants but its use has been rare on monocotyledons. In this paper, we report that A. tumefaciens transformed embryonic orchid tissue and caused alteration in its normal developmental course.The second paper details the DNA fingerprinting of tissue from Aplectrum hymale, a terrestrial orchid native to this climate. Three populations of A. hymale have been sampled and DNA extracted from the tissue samples. RAPD primers were used to prime PCR amplifications of random sequences of the DNA and the amplified DNA was visualized by gel electrophoresis. Loci of the resulting bands were treated as potentially multiallelic gene loci and heterozygosity between and within subpopulations was calculated. We report that the three populations could be partially differentiated by this procedure and that the two populations located nearest to each other yielded the least between -ubpopulation heterozygosity. We report very high levels of genetic diversity between individuals within small subpopulations in spite of the fact that these subpopulations are considered to be primarily clonal in reproductive nature.
Department of Biology

APA, Harvard, Vancouver, ISO, and other styles

34

Cheung, Kin Lok. "Investigation of (3-mercaptopropyl) trimethoxysilane (MPTS)-modified surface and DNA microarray for genotyping of traditional Chinese medicinal plants /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202003%20CHEUNGK.

Full text

Abstract:

Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2003.
Includes bibliographical references (leaves 103-111). Also available in electronic version. Access restricted to campus users.

APA, Harvard, Vancouver, ISO, and other styles

35

Chung, Denise T. "The development of novel STR miniplex primer sets for the analysis of degraded and compromised DNA samples." Ohio : Ohio University, 2004. http://www.ohiolink.edu/etd/view.cgi?ohiou1097609199.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Honing, Jennifer. "Evaluation and implementation of DNA-based diagnostic methodology to distinguish wheat genotypes." Thesis, Link to the online version, 2007. http://hdl.handle.net/10019/638.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Santana, de Oliveira Nilmara. "Variabilidade genética em populações de Ricinus communis (Euphorbiaceae) pela metodologia de DAF (DNA Amplification Fingerprinting." Universidade Federal de Pernambuco, 2004. https://repositorio.ufpe.br/handle/123456789/6676.

Full text

Abstract:

Made available in DSpace on 2014-06-12T18:06:46Z (GMT). No. of bitstreams: 2 arquivo6319_1.pdf: 813959 bytes, checksum: ac0fdfeef1e3a6adeb19be879b540aad (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2004
O Brasil ocupa mundialmente a terceira posição na produção de mamona (Ricinus communis L.). Embora existam muitos estudos moleculares voltados às proteínas expressas nas sementes, não existem até o momento análises com marcadores moleculares avaliando a diversidade genética dessa espécie. O presente trabalho estudou a variabilidade genética de 16 genótipos de populações subespontâneas de mamona adquiridas através de coletas em quatro diferentes localidades de Pernambuco e em duas outras regiões do Brasil, comparando-as a acessos cultivados de quatro outros países. Para isso a metodologia de DAF (DNA amplification Fingerprinting) foi utilizada, permitindo a geração de em média 14 bandas por primer, sendo 10,72 polimórficas, a partir de 11 primers. Para a construção da matriz de dados 143 bandas foram analisadas, perfazendo 2.288 caracteres. A análise filogenética de máxima parsimônia revelou uma diversidade genética significativa, entre genótipos da mesma população, considerando os diferentes pontos de coleta no Brasil, bem como comparativamente com acessos cultivados no exterior. O estudo confirma a variabilidade sugerida pelos melhoristas para as populações subespontâneas de mamona e demonstra a eficiência deste tipo de marcador para estudos de caracterização de germoplasma desta importante cultura vegetal.

APA, Harvard, Vancouver, ISO, and other styles

38

Van, Winkle Carolyn. "Forensic DNA Extraction Strategies for PCR Analysis." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc278269/.

Full text

Abstract:

There is a transition nationwide on the analysis of forensic evidentiary stains containing biological material from traditional serology to Polymerase Chain Reaction (PCR) methodologies. The increased sensitivity of PCR, the limited number of alleles at each locus, and the necessity of producing unambiguous data for entry into the FBI's Combined DNA Index System make this study of extraction procedures of utmost importance. A "single tube" extraction procedure for blood stains collected onto FTA™ paper and a modified differential nonorganic extraction method from spermatozoa containing mixed stains were analyzed and compared. The extraction success was evaluated by amplification and typing of the amplified fragment length polymorphism, D1S80. These modifications of the nonorganic method utilized gave an improved separation of the spermatozoa-containing mixed stains.

APA, Harvard, Vancouver, ISO, and other styles

39

Beatini, Salvatore J. "Using DNA fingerprinting to assess genetic structure of the vernal pool amphibian rana sylvatica." Link to electronic thesis, 2003. http://www.wpi.edu/Pubs/ETD/Available/etd-0428103-153026.

Full text

Abstract:

Thesis (M.S.)--Worcester Polytechnic Institute.
Keywords: wood frog; vernal pool conservation; fragmented habitat; Rana sylvatica; DNA fingerprinting. Includes bibliographical references (p. 38-40).

APA, Harvard, Vancouver, ISO, and other styles

40

Fernando, Raniero. "Brood parasitism and genetic parentage in Goldeneye ducks, an analysis using DNA fingerprinting." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0027/MQ51602.pdf.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Dawe, Yvonne M. "DNA fingerprinting : a tool for determining genetic variability and strain relationships in poultry." Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=63909.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Goll, Melanie [Verfasser]. "Nachweis und DNA-Fingerprinting von Escherichia-coli-O157-Stämmen bei Pferden / Melanie Goll." Wetteberg : VVB Laufersweiler, 2005. http://d-nb.info/976072742/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Gordon, Ann Elizabeth-Chamberlain. "Criminal paternity DNA testing of microscopically-identified chorionic villi in formalin-fixed paraffin-embedded products of conception." Diss., Connect to online resource - MSU authorized users, 2006.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

44

Williams, Timothy Roy. "Reeves' muntjac : a molecular genetic study of an invading species." Thesis, University of Kent, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283343.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Dawson, Robert J. G. "A molecular examination of some enigmatic birds." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335505.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Ridley, Anne McAlpine. "Epidemiological typing of Listeria monocytogenes." Thesis, Open University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361592.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Ramsey, Heather C. "Comparisons of mitochondrial DNA from ancient and modern Miami Indian populations." Virtual Press, 1999. http://liblink.bsu.edu/uhtbin/catkey/1133738.

Full text

Abstract:

The purpose of this research endeavor was to determine the extent of genetic relatedness between an ancient and modern Miami Indian population. The modern Miami Indian nation in Indiana is currently in the process of regaining the federal recognition which was lost in the mid 1800's when part of the tribe was forced to relocate. A close genetic relationship between a modern and known ancient population could considerably strengthen the case to regain federal recognition. The human skeletal remains used for this experiment were excavated after partial exposure by flooding between 1989-1993 along the banks of the Mississinewa River in Wabash County. Through ethnohistoric dating techniques, the remains have been shown to represent a Miami Indian population living between 1790-1820. In order to yield amplifiable DNA several methods of isolation were attempted and compared. CTAB and phenol/chloroform/isoamyl alcohol (24:24:1) and a silica based purification method provided the best results yielding approximately 50-100 ng of amplifiable DNA from 3 of the 4 individuals. Purification of the DNA was found to be necessary following both isolation (Elu-Quik) prior to PCR amplification and after PCR but prior to sub-cloning(Gene-Clean). Regions of the mitochondrial DNA (mtDNA) genome of isolated DNA were amplified using primers which are specific for the HIizcIl and AIui regions of the mtDNA genome. Although the mtDNA proved to be somewhat amplifiable, it was still too fragmented to be cloned, which prevented genetic analysis and comparison of the two populations. As a result, a discussion of alternative methods for looking at relatedness between populations has been included.
Department of Biology

APA, Harvard, Vancouver, ISO, and other styles

48

Stanley, Dianne M. "Estimation of relatedness of thoroughbreds and eight breeds of horses using DNA fingerprinting of whole blood." Thesis, This resource online, 1993. http://scholar.lib.vt.edu/theses/available/etd-01312009-063247/.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Morin, Geneviève. "Metabolite fingerprinting tools to detect differences between transgenic and conventional crops." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=101629.

Full text

Abstract:

A concern in transgenic crops is the potential risk posed by unintended effects which could result from genetic transformation. The objective of this work was to develop an untargeted approach that could characterize transgenic crops, as well as conventional crops, at the molecular level. An experimental approach was designed and used to compare conventional and transgenic soybean varieties. Varieties were compared using their metabolite fingerprints obtained by reverse-phase high performance liquid chromatography (HPLC) and both the analytical and biological variability were assessed. Multivariate and univariate statistical analyses were applied to the data to detect significant differences between the varieties. It was found that transgenic variety PS 46 RR was the most different variety analyzed and that it differed most from Mandarin (Ottawa) and AC Dundas. The statistical analyses also determined that PS 46 RR differed more from the conventional varieties tested than 2601R did.

APA, Harvard, Vancouver, ISO, and other styles

50

Guerasimova, Anna. "Novel experimental approaches to DNA characterisation by oligonucleotide fingerprinting application of peptide nucleic acids /." [S.l. : s.n.], 2000. http://www.diss.fu-berlin.de/2000/107/index.html.

Full text

APA, Harvard, Vancouver, ISO, and other styles

Dissertations / Theses on the topic 'Aging DNA DNA fingerprinting'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles