Relevant bibliographies by topics / Agpat2

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Academic literature on the topic 'Agpat2'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Agpat2"

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LU, Biao, Yan J. JIANG, Yaling ZHOU, Fred Y. XU, Grant M. HATCH та Patrick C. CHOY. "Cloning and characterization of murine 1-acyl-sn-glycerol 3-phosphate acyltransferases and their regulation by PPARα in murine heart". Biochemical Journal 385, № 2 (2005): 469–77. http://dx.doi.org/10.1042/bj20041348.

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AGPAT (1-acyl-sn-glycerol 3-phosphate acyltransferase) exists in at least five isoforms in humans, termed as AGPAT1, AGPAT2, AGPAT3, AGPAT4 and AGPAT5. Although they catalyse the same biochemical reaction, their relative function, tissue expression and regulation are poorly understood. Linkage studies in humans have revealed that AGPAT2 contributes to glycerolipid synthesis and plays an important role in regulating lipid metabolism. We report the molecular cloning, tissue distribution, and enzyme characterization of mAGPATs (murine AGPATs) and regulation of cardiac mAGPATs by PPARα (peroxisome-proliferator-activated receptor α). mAGPATs demonstrated differential tissue expression profiles: mAGPAT1 and mAGPAT3 were ubiquitously expressed in most tissues, whereas mAGPAT2, mAGPAT4 and mAGPAT5 were expressed in a tissue-specific manner. mAGPAT2 expressed in in vitro transcription and translation reactions and in transfected COS-1 cells exhibited specificity for 1-acyl-sn-glycerol 3-phosphate. When amino acid sequences of five mAGPATs were compared, three highly conserved motifs were identified, including one novel motif/pattern KX2LX6GX12R. Cardiac mAGPAT activities were 25% lower (P<0.05) in PPARα null mice compared with wild-type. In addition, cardiac mAGPAT activities were 50% lower (P<0.05) in PPARα null mice fed clofibrate compared with clofibrate fed wild-type animals. This modulation of AGPAT activity was accompanied by significant enhancement/reduction of the mRNA levels of mAGPAT3/mAGPAT2 respectively. Finally, mRNA expression of cardiac mAGPAT3 appeared to be regulated by PPARα activation. We conclude that cardiac mAGPAT activity may be regulated by both the composition of mAGPAT isoforms and the levels of each isoform.
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Sukumaran, Suja, Robert I. Barnes, Abhimanyu Garg, and Anil K. Agarwal. "Functional characterization of the human 1-acylglycerol-3-phosphate-O-acyltransferase isoform 10/glycerol-3-phosphate acyltransferase isoform 3." Journal of Molecular Endocrinology 42, no. 6 (2009): 469–78. http://dx.doi.org/10.1677/jme-09-0010.

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Synthesis of phospholipids can occur de novo or via remodeling of the existing phospholipids. Synthesis of triglycerides, a form of energy storage in cells, is an end product of these pathways. Several 1-acylglycerol-3-phosphate-O-acyltransferases (AGPATs) acylate lysophosphatidic acid (LPA) at the sn-2 (carbon 2) position to produce phosphatidic acid (PA). These enzymes are involved in phospholipids and triglyceride synthesis through an evolutionary conserved process involving serial acylations of glycerol-3-phosphate. We cloned a cDNA predicted to be an AGPAT isoform (AGPAT10). This cDNA has been recently identified as glycerol-3-phosphate-O-acyltransferase isoform 3 (GPAT3). When this AGPAT10/GPAT3 cDNA was expressed in Chinese Hamster ovary cells, the protein product localizes to the endoplasmic reticulum. In vitro enzymatic activity using lysates of human embryonic kidney-293 cells infected with recombinant AGPAT10/GPAT3 adenovirus show that the protein has a robust AGPAT activity with an apparent Vmax of 2 nmol/min per mg protein, but lacks GPAT enzymatic activity. This AGPAT has similar substrate specificities for LPA and acyl-CoA as shown for another known isoform, AGPAT2. We further show that when overexpressed in human Huh-7 cells depleted of endogenous AGPAT activity by sh-RNA-AGPAT2-lentivirus, the protein again demonstrates AGPAT activity. These observations strongly suggest that the cDNA previously identified as GPAT3 has AGPAT activity and thus we prefer to identify this clone as AGPAT10 as well.
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Agarwal, Anil K., Suja Sukumaran, Rene Bartz, Robert I. Barnes, and Abhimanyu Garg. "Functional characterization of human 1-acylglycerol-3-phosphate-O-acyltransferase isoform 9: cloning, tissue distribution, gene structure, and enzymatic activity." Journal of Endocrinology 193, no. 3 (2007): 445–57. http://dx.doi.org/10.1677/joe-07-0027.

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Most cells synthesize their glycerophospholipids and triglycerides (TG) to maintain the cellular integrity and to provide energy for cellular functions. The phospholipids are synthesized de novo in cells through an evolutionary conserved process involving serial acylations of glycerol-3-phosphate. Several isoforms of the enzyme 1-acylglycerol-3-phosphate-O-acyltransferase (AGPAT) acylate lysophosphatidic acid at the sn-2 position to produce phosphatidic acid. We cloned a cDNA predicted to be an AGPAT isoform and designated it AGPAT9. The human AGPAT9 gene spans across 14 exons and encodes for a polypeptide of 534 amino acids. AGPAT9 is highly expressed in the lung and spleen, followed by leukocyte, omental adipose tissue, and placenta. In the Chinese Hamster Ovary (CHO), cell lysates overexpressing AGPAT9, we observed AGPAT activity but not the lysophosphatidylcholine acyltransferase activity. When AGPAT9 is coexpressed with AGPAT1 in CHO cells, both the isoforms localize to the endoplasmic reticulum (ER) and occupy the same ER domain as AGPAT1. Despite substitution of asparagine with proline in the NHX4D motif and arginine with cysteine in the EGTR motif, AGPAT9 retains AGPAT activity suggesting that residues asparagine and arginine in the NHX4D and EGTR motifs respectively are not essential for the enzymatic activity. Based on the X-ray crystallographic structure of a related acyltransferase, squash gpat, a model is proposed in which a hydrophobic pocket in AGPAT9 accommodates fatty acyl chains of both substrates in an orientation, whereas the HX4D motif participates in catalysis. Based on the activity and expression pattern of AGPAT9 in the lung and spleen, this novel isoform could be implicated in the biosynthesis of phospholipids and TG in these tissues.
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Agarwal, Anil K., Vinaya Simha, Elif Arioglu Oral, et al. "Phenotypic and Genetic Heterogeneity in Congenital Generalized Lipodystrophy." Journal of Clinical Endocrinology & Metabolism 88, no. 10 (2003): 4840–47. http://dx.doi.org/10.1210/jc.2003-030855.

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Abstract Congenital generalized lipodystrophy (CGL) is a rare autosomal recessive disorder characterized by near complete absence of adipose tissue from birth. Recently, mutations in 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2) and Berardinelli-Seip congenital lipodystrophy 2 (BSCL2) genes were reported in pedigrees linked to chromosomes 9q34 and 11q13, respectively. There are limited data regarding phenotypic differences between the various subtypes of CGL. Furthermore, whether there are additional loci for CGL remains unknown. Therefore, we genotyped 45 pedigrees with CGL for AGPAT2 and BSCL2 loci and compared the phenotypes in the various subtypes. Twenty-six pedigrees harbored mutations, including seven novel variants, in the AGPAT2 gene, and 11 pedigrees harbored mutations in the BSCL2 gene, including five novel variants. Eight pedigrees had no substantial alterations in either gene. Of these, three informative pedigrees showed no linkage to markers spanning the AGPAT2 and BSCL2 loci, and in six of the affected subjects, the transcripts of AGPAT2 and BSCL2 were normal. All subtypes of CGL showed high prevalence of diabetes, hypertriglyceridemia, and acanthosis nigricans. However, patients with BSCL2 mutations had lower serum leptin levels, an earlier onset of diabetes, and higher prevalence of mild mental retardation compared with other subtypes. We conclude that besides AGPAT2 and BSCL2, there may be additional loci for CGL. The genetic heterogeneity in CGL patients is accompanied by phenotypic heterogeneity.
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Jin, Jing, Lingfeng Cao, Zhuhui Zhao, et al. "Novel BSCL2 gene mutation E189X in Chinese congenital generalized lipodystrophy child with early onset diabetes mellitus." European Journal of Endocrinology 157, no. 6 (2007): 783–87. http://dx.doi.org/10.1530/eje-07-0393.

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ContextCongenital generalized lipodystrophy (CGL) is a rare and heterogeneous disease of autosomal recessive inheritance. Until now, no genetic findings had been reported in Chinese patients with CGL.ObjectiveTo analyze Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2) and 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2) gene variation in a Chinese boy with CGL and his family.Design, setting, and participantsAll exons of BSCL2 and AGPAT2 with adjacent intron–exon junctions were analyzed using direct sequencing.Main outcome measuresSequences of each exon and nearby intron of the BSCL2 and AGPAT2 genes of the family members were compared with the gene bank genomic sequences.ResultsDNA sequence analysis of the entire coding regions and surrounding uncoding regions disclosed a novel homozygous G→T mutation at nucleotide 909 in exon 5 of the BSCL2 gene in the affected child. A heterozygous state of the G→T mutation of the BSCL2 gene was also found in other family members. This mutation predicts the substitution of glutamic acid at codon 189 by the stop codon (Glu189X or E189X). No variation was found in the AGPAT2 gene.ConclusionE189X is a novel BSCL2 gene mutation that contributes to CGL formation in a family of Chinese origin.
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Gomes, Karina Braga, Ana Paula Fernandes, Alessandro Clayton Souza Ferreira, et al. "Mutations in the Seipin and AGPAT2 Genes Clustering in Consanguineous Families with Berardinelli-Seip Congenital Lipodystrophy from Two Separate Geographical Regions of Brazil." Journal of Clinical Endocrinology & Metabolism 89, no. 1 (2004): 357–61. http://dx.doi.org/10.1210/jc.2003-030415.

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Berardinelli-Seip congenital lipodystrophy (BSCL) is characterized by a near total congenital absence of fat and predisposition to develop diabetes mellitus. In this study, we investigated the presence of mutations in the Seipin and 1-acylglycerol phosphate acyltransferase 2 (AGPAT2) genes in 32 affected subjects with BSCL from 17 consanguineous pedigrees living in two separate geographical regions, the northeastern and southeastern regions, of Brazil. All, except one, of the 22 BSCL subjects from 15 families living in the northeastern region were found to have a homozygous 669insA mutation in the Seipin gene. In contrast, all 10 BSCL subjects from two families living in the southeastern region were found to a have a homozygous 1036-bp deletion including exons 3 and 4 of AGPAT2. These results support genetic heterogeneity among BSCL patients in Brazil. Our finding of a single mutation in the Seipin and AGPAT2 genes in the pedigrees from the northeastern and southeastern regions, respectively, will be useful in genetic counseling of subjects from these large pedigrees from Brazil.
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Barra, Cristiane B., Roberta D. Savoldelli, Thais D. Manna, et al. "Síndrome de Berardinelli-Seip: descrição genética e metabólica de cinco pacientes." Arquivos Brasileiros de Endocrinologia & Metabologia 55, no. 1 (2011): 54–59. http://dx.doi.org/10.1590/s0004-27302011000100007.

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OBJETIVO: Descrever o perfil genético e metabólico de portadores da síndrome de Berardinelli-Seip (BSCL) acompanhados no Instituto da Criança do HC-FMUSP. SUJEITOS E MÉTODOS: Pacientes com as características clínicas da BSCL (n = 5), todas do sexo feminino, foram avaliadas com dosagens de glicose e insulina, lípides, leptina, enzimas hepáticas, análise de DNA, ultrassonografia abdominal. RESULTADOS: A deficiência de leptina e a hipertrigliceridemia foram constatadas nas cinco pacientes. Três evoluíram para diabetes melito (DM). Quatro tiveram mutação no gene AGPAT2 e uma no gene CAV1. CONCLUSÃO: As alterações metabólicas mais precoces foram a hipertrigliceridemia e a resistência insulínica, culminando no surgimento do DM à época da puberdade, sendo as mutações no gene AGPAT2 as mais frequentes em nossa casuística.
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Magre, J., M. Delepine, L. Van Maldergem, et al. "Prevalence of Mutations in AGPAT2 Among Human Lipodystrophies." Diabetes 52, no. 6 (2003): 1573–78. http://dx.doi.org/10.2337/diabetes.52.6.1573.

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Subauste, A. R., A. K. Das, X. Li, et al. "Alterations in Lipid Signaling Underlie Lipodystrophy Secondary to AGPAT2 Mutations." Diabetes 61, no. 11 (2012): 2922–31. http://dx.doi.org/10.2337/db12-0004.

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Simha, Vinaya, and Abhimanyu Garg. "Phenotypic Heterogeneity in Body Fat Distribution in Patients with Congenital Generalized Lipodystrophy Caused by Mutations in the AGPAT2 or Seipin Genes." Journal of Clinical Endocrinology & Metabolism 88, no. 11 (2003): 5433–37. http://dx.doi.org/10.1210/jc.2003-030835.

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Abstract Congenital generalized lipodystrophy (CGL) is a rare autosomal recessive syndrome characterized by extreme paucity of adipose tissue since birth, acanthosis nigricans, severe insulin resistance, marked hypertriglyceridemia, and early-onset diabetes mellitus. Recently, we reported mutations in the 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2) gene in CGL pedigrees linked to chromosome 9q34 (CGL1 subtype), and mutations in the Seipin gene were reported in pedigrees linked to chromosome 11q13 (CGL2 subtype). Whether the two subtypes have differences in body fat distribution has not been investigated. We, therefore, compared whole-body adipose tissue distribution by magnetic resonance imaging in 10 CGL patients, of whom seven (six females, one male) had CGL1 and three (two males, one female) had CGL2. Both subtypes had marked lack of metabolically active adipose tissue located at most sc, intermuscular, bone marrow, intraabdominal, and intrathoracic regions. Paucity of mechanical adipose tissue in the palms, soles, orbits, scalp, and periarticular regions was noted in CGL2, whereas it was well preserved in CGL1 patients. We conclude that CGL patients with Seipin mutations have a more severe lack of body fat, which affects both metabolically active and mechanical adipose tissue, compared with patients with mutations in the AGPAT2 gene.
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Dissertations / Theses on the topic "Agpat2"

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Ramanathan, Nardev. "Molecular basis of inherited lipodystrophies caused by mutations in AGPAT2 and LMNA." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607936.

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Boutet, Emilie. "La lipodystrophie congénitale généralisée de Berardinelli-Seip : pathologie moléculaire et cellulaire." Paris 6, 2008. http://www.theses.fr/2008PA066119.

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La lipodystrophie congénitale de Berardinelli-Seip (BSCL) est une maladie rare caractérisée par une quasi-absence de tissu adipeux (TA) et une insulino-résistance sévère. Elle est transmise de façon récessive et résulte dans environ 95% des cas de mutations de BSCL2, le gène codant la seipine de fonction inconnue, ou de AGPAT2, codant l’enzyme 1-acylglycérol-3-phosphate acyltransférase qui catalyse la formation de l’acide phosphatidique. Nous avons récemment impliqué un nouveau gène, CAV1 codant la Cavéoline-1, dans l’étiologie de la BSCL à la suite de la découverte d’une mutation homozygote non-sens (p. Glu38X) chez une patiente Brésilienne. CAV1 est un composant majeur des caveolae qui jouent un rôle important dans la réponse à l’insuline et l’homéostasie lipidique. Parallèlement, nous avons étudié le mécanisme physiopathologique de la BSCL liée à la seipine. Nos résultats indiquent que la seipine joue probablement un rôle dans le TA car son expression est prédominante dans plusieurs dépôts adipeux murins et est régulée dans des conditions capables de modifier la masse adipeuse. De plus, elle est fortement induite en fin du processus de différenciation adipocytaire. L’invalidation partielle de la seipine dans une lignée adipocytaire murine induit des altérations de la morphologie des gouttelettes lipidiques (GL) et une mort par apoptose des préadipocytes. D’autre part, l’étude des lymphocytes des patients a révélé que la seipine est impliquée dans le biosynthèse lipidique et en particulier dans la voie liant la 9-désaturation des acides gras à la formation de la GL. Ainsi, nos résultats impliquent la seipine dans le métabolisme lipidique et la formation de la GL et sont donc compatibles avec l’atteinte primaire du tissu adipeux dans la BSCL. Cependant, il est probable que la seipine intervienne dans d’autres tissus et la découverte de sa fonction précise permettra de mieux comprendre la physiologie de la GL. L’ensemble de ces données fournit de nouvelles avancées dans la compréhension du mécanisme de la BSCL.
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Ouellette, Catherine. "Effets des polymorphismes des gènes MGLL, GPAM, AGPAT3 et AGPAT4 sur la variation des lipides plasmatiques en réponse à une supplémentation en acides polyinsaturés oméga-3 d'origine marine." Master's thesis, Université Laval, 2014. http://hdl.handle.net/20.500.11794/25632.

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La consommation d’acides gras polyinsaturés (AGPI) oméga-3 (n-3) d’origine marine diminue les niveaux de triglycérides (TG) plasmatiques et augmente les concentrations de cholestérol (C) des lipoprotéines de faible densité (LDL) ainsi que la taille des particules LDL. On observe une importante variabilité interindividuelle dans la réponse à la supplémentation, qui pourrait être expliquée par des facteurs génétiques. Les effets de polymorphismes (SNPs) de gènes impliqués dans les voies métaboliques de dégradation et de synthèse des TG ont été étudiés chez des sujets en santé, mais souffrant d’embonpoint, ayant reçu une supplémentation de 5 g par jour d’huile de poisson. Des interactions génotype*supplémentation ont été observées pour des SNPs des gènes GPAM, AGPAT4 et MGLL en relation avec les niveaux de lipides plasmatiques. Ces résultats suggèrent que ces SNPs modulent la réponse des lipides plasmatiques à une supplémentation en AGPI n-3 d’origine marine et pourraient expliquer une partie de variabilité interindividuelle observée.
Omega-3 (n-3) polyunsaturated fatty acids (PUFA) from marine sources are known to lower plasma triglyceride (TG) levels and increase low-density lipoprotein (LDL) cholesterol (C) as well as LDL particle size. However, a large inter-individual variability is observed, which could be explained by genetic factors. The effects of single nucleotide polymorphisms (SNPs) of genes within the TG synthesis or metabolism pathways have been studied in a cohort of healthy overweight adults receiving 5 g per day of marine n-3 PUFA. Genotype*supplementation interaction effects on plasma lipids were observed for SNPs in GPAM, AGPAT4 and MGLL genes. These results suggest that these SNPs modulate the plasma lipid response to a marine n-3 PUFA supplementation and could explain part of the inter-individual variability observed.
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Kappelt, Frederik [Verfasser]. "Synthese von Ester- und Etherlipiden durch die GPAT/AGPAT-Proteinfamilie und Lipin in Dictyostelium / Frederik Kappelt." Kassel : Universitätsbibliothek Kassel, 2021. http://d-nb.info/1240910622/34.

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Piwowarski, Patrick. "FTIR-spektroskopische Untersuchungen am Phytochrom Agp2." Doctoral thesis, Humboldt-Universität zu Berlin, Lebenswissenschaftliche Fakultät, 2017. http://dx.doi.org/10.18452/17788.

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In der vorliegenden Arbeit wurde der lichtinduzierte Reaktionszyklus des bakteriellen Phytochroms Agp2 aus Agrobacterium tumefaciens mit FTIR‑ und UV‑Vis‑Spektroskopie untersucht. Der Photorezeptor besteht aus einem photosensorischen Modul und einer signalgebenden Histidin-Kinase-Domäne. Das photosensorische Modul bindet das Tetrapyrrol Biliverdin als Chromophor. Der Grundzustand von Agp2 (Pfr, 750 nm) ist gegenüber dem lichtaktivierten Zustand (Pr, 700 nm) rotverschoben, weshalb Agp2 den Bathyphytochromen zugeordnet wird. Die Untersuchungen erfolgten unter Verwendung von Isotopenmarkierung, H/D-Austauschexperimenten und ortsspezifischer Mutagenese. Daraus ließen sich folgende molekulare Änderungen charakterisieren, welche im Reaktionszyklus von Agp2 erfolgen: Die lichtinduzierte Isomerisierung des Chromophors führt zu einem Übergang vom Pfr- in den Pr-Zustand, wobei zwei Intermediate, Lumi‑F und Meta‑F, durchlaufen werden. Neben der Konformationsänderung des Chromophor‑D‑Rings ist auch die C‑Ring-Propionsäureseitenkette an der Photoreaktion beteiligt. Die C-Ring-Propionsäureseitenkette ist im Pfr-Zustand protoniert und wird im Übergang von Meta-F zu Pr deprotoniert. Der Pr-Zustand weist eine pH-Abhängigkeit auf, welche auf die pH-abhängige Ladung des Histidins 278 der Chromophortasche zurückzuführen ist. Je nach Ladung des Histidins 278 wird die Keto‑ bzw. Enolform der C(19)=O‑Gruppe des D‑Rings stabilisiert. Die Keto/Enol-Tautomerie ist auf eine innerhalb des Chromophors erfolgende Protontranslokation zurückzuführen und moduliert die Relaxation in den Pfr-Zustand. Änderungen der Amid-I-Absorption im Pfr-Pr-Übergang werden der Umstrukturierung der Tongue-Region des photosensorischen Moduls von einer Alpha-helikalen zu einer Beta‑Faltblatt-Struktur zugeordnet. Diese Strukturänderung wird als möglicher Weg der proteininternen Signaltransduktion zwischen photosensorischem und signalgebendem Modul vorgeschlagen.
In this thesis the light-induced reaction cycle of the bacterial phytochrome Agp2 from Agrobacterium tumefaciens was investigated using FTIR and UV‑vis spectroscopy. The photoreceptor comprises a photosensitive module and a signalling histidine kinase domain. The photosensitive module binds the biliverdin tetrapyrrol as chromophore. The Agp2 ground state (Pfr, 750 nm) is red-shifted in comparison with its light-activated state (Pr, 700 nm). Therefore, Agp2 is assigned to the group of bathy phytochromes. The investigations were conducted using isotopically labelled protein, labelled chromophore as well as hydrogen‑deuterium (H‑D) exchange and site-directed mutagenesis. Based on these the following molecular changes could be characterized that occur in the reaction cycle of Agp2: The light-induced isomerization of the chromophore leads to a transition from the Pfr to the Pr state, involving two intermediates, Lumi-F and Meta-F. Besides conformational changes of the chromophore D-ring, the C-ring propionic side chain is involved in the photoreaction as well. The C-ring propionic side chain is protonated in the Pfr state and gets deprotonated in the Meta-F to Pr transition. The Pr state exhibits pH‑dependent alterations which can be explained by pH dependent polarity changes of histidine 278 in the chromophore pocket. Depending on the charge of histidine, the D‑ring C(19)=O group is stabilized either in keto or enol form. The keto/enol tautomerism involves a proton translocation within the chromophore and modulates the relaxation to the Pfr state. The changes in the amide I region in the Pfr-Pr transition are associated with an alpha‑helix to beta‑sheet secondary structure change of the PHY domain tongue‑region. This structural change is proposed as the potential path of signal transduction between the photosensitive and the signalling module.
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Caudy, Nada. "Etude fonctionnelle de deux marqueurs régionaux du cerveau chez la souris." Phd thesis, Université Paris Sud - Paris XI, 2011. http://tel.archives-ouvertes.fr/tel-00868783.

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Ce travail porte sur l'étude fonctionnelle de deux gènes préférentiellement exprimés dans deux régions du cerveau touchées par des pathologies neurodégénératives : Capucine, un marqueur du striatum, structure qui dégénère au cours de la maladie de Huntington et Agpat4, un marqueur de l'aire tegmentaire ventrale et de la substance noire compacte, dont les neurones dopaminergiques sont sélectivement atteints lors de la maladie de Parkinson. Des lignées de souris invalidées pour ces gènes ont été générées au laboratoire et au cours de ma thèse j'ai procédé à leur caractérisation. L'expression striatale du gène de la Capucine étant significativement diminuée dans des modèles murins de la maladie de Huntington, nous avons souhaité évaluer son rôle éventuel dans la pathogenèse de cette maladie. Pour ce faire, nous avons examiné, dans le cadre d'une collaboration, l'effet du knock-out et de la surexpression du gène de la Capucine sur la vulnérabilité des neurones striataux à un fragment de la Huntingtine mutée dans un modèle murin de la maladie de Huntington. Les données montrent que la Capucine n'a pas d'effet significatif sur la toxicité du fragment de la Huntingtine mutée dans le modèle étudié.La protéine Agpat4 présente des homologies de séquence avec des acyltransférases impliquées dans le métabolisme des phosphoglycérides. J'ai réalisé des études d'expression par différentes techniques de biologie moléculaire qui montrent que le gène d'Agpat4 est exprimé dans la plupart des tissus catécholaminergiques. Pour déterminer l'activité endogène d'Agpat4 et son rôle physiologique dans les tissus où elle est exprimée, j'ai comparé le métabolome de tissus de souris invalidées pour le gène d'Agpat4 et sauvages par chromatographie en phase liquide couplée à la spectrométrie de masse. Mes résultats indiquent que l'invalidation du gène d'Agpat4 perturbe le métabolisme non seulement de différentes classes de lipides, notamment les lysophosphatidyléthanolamines, mais aussi celui des catécholamines.
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Rosa, Mario Borges. "Erros de medicação em um hospital de referência de Minas Gerais, 2001." Universidade Federal de Minas Gerais, 2002. http://hdl.handle.net/1843/AGPA-5L6F6B.

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Aiming to evaluate the use of drugs and high-risk conditions predisposing to medication errors, there was a cross-sectional study in 4026 prescriptions containing potentially dangerous drugs (MPP) in a public hospital of reference of Minas Gerais, the period August-September 2001. The readability analysis showed that 19.4% of prescriptions were classified as barely legible and illegible, which increases the risk of measurement errors. The type of limitation was classified into three options: handwriting, mixed or pre-typed and evaluated concurrently readability. There's a higher chance of pre-typed prescriptions delivered the best results in terms of readability. There's a higher chance of handwritten prescriptions are illegible or barely legible, increasing the risk of mistakes. Prescription Pre-typed showed the best results in terms of readability. We evaluated the identification of prescribers emphasizing that in 39.70% of the cases it was difficult or impossible to know which was the author of the prescription. The 7.148MPP prescribed were examined in relation to legibility, pharmaceutical form, concentration, route of administration, range and rate of infusion. 3177 discrepancies were observed with a convergence of 89.1% in four types of problems: lack of concentration and placement of the pharmaceutical form, concentration is difficult to read and doubtful. The most frequent discrepancy was a lack of placing the merger with 49.76% of the records. The Intensive Care Unit (ICU), the Intensive Care Unit (ICU) and Neurology were the sectors with the highest number of problems per prescription. It was noted discrepancies in the concentration of five MPP: hiparina, fentanyl, midazolam, nalbuphine and pancuronium. There were 23 prescribing errors with the following MPP: heparin, midazolam, digoxin, fentanyl and nalbuphine
Com o objetivo de avaliar a utilização de medicamentos de alto risco e condições predisponentes a erros de medicação, realizou-se um estudo descritivo transversal, em 4026 prescrições que continham medicamentos potencialmente perigosos (MPP), em um hospital público de referencia de Minas Gerais, no período de agosto a setembro de 2001. A análise da legibilidade mostrou que 19,4% das prescrições foram classificadas como pouco legíveis e ilegíveis, situação que aumenta o risco de erros de medição. O tipo de prescrição foi classificado em três opções: escrita à mão, mista ou pré-digitada e avaliada concomitantemente a legibilidade. Observou-se maior chance das prescrições pré-digitada apresentou os melhores resultados quanto à legibilidade. Observou-se maior chance das prescrições escritas à mão serem pouco legíveis ou ilegíveis, aumentando o risco de enganos. A prescrição pré-digitada apresentou os melhores resultados quanto à legibilidade. Avaliou-se a identificação dos prescritores destacando-se que, em 39,70% dos casos, houve dificuldade ou foi impossível saber qual o autor da prescrição. Os 7.148MPP prescritos foram examinados em relação à legibilidade, forma farmacêutica, concentração, via de administração, intervalo e taxa de infusão. Foram observadas 3.177 discrepâncias com uma convergência de 89,1% em quatro tipos de problemas: falta de colocação da concentração e forma farmacêutica, pouca legibilidade e concentração duvidosa. A discrepância mais freqüente foi falta de colocação da concentração com 49,76% dos registros. O Centro de Tratamento Intensivo (CTI), a Unidade de Tratamento Intensivo (UTI) e a Neurologia foram os setores que apresentaram maior número de problemas por prescrição. Notou-se a concentração das discrepâncias em cinco MPP: hiparina, fentanil, midazolam, nalbufina e pancurônio. Ocorreram 23 erros de prescrição com os seguintes MPP: heparina, midazolam, digoxina, nalbufina e fentanil.
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Abrantes, Patricia de Magalhaes. "Estudo da prescrição de antibacterianos de uso sistêmico em unidades da Gerência de Saúde Centro Sul (GERSA CS) - Belo Horizonte MG." Universidade Federal de Minas Gerais, 2003. http://hdl.handle.net/1843/AGPA-5RYP49.

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The antimicrobial use has been raising discussions concerning the rationality of its use, in order to prevent the appearance of strains of resistant microorganisms. This work aim to investigate the use of antibacterial of systemic use in health centers of the Municipal Health Department in Belo Horizonte. It is a cross-sectional study, with two stages. In the first one, it has been made an evaluation of the prescriptions dispensed during March 2002, using the indicators of the World Health Organization. On the second one, it was made an evaluation of medical records related to a sample of the prescriptions. It has been verified that in average 20.0% of the prescriptions dispensed had at least one antibacterial of systemic use and among them, approximately 46.0% originated from non-municipal health centers. The pediatrics presented the largest rate of antimicrobial prescription. Amoxicillin was the more often antibacterial prescript, followed by benzylpenicillin benzathine, the association of sulfamethoxazole+trimethoprim and the erythromycin. It was observed, in the prescriptions, rates of record of technical data (dose, intervals between doses, administration and period of treatment) varying from 23.6 to 99.6%. In about 10.0% of the prescriptions, there was no definition of the period of treatment for the antimicrobial agent. Amoxicillin was the antibacterial that presented the best results related to the indicators used to assess quality of prescriptions and the erythromycin the worst ones. Related to the medical records, the diagnostics hypothesis registered with larger frequency was tonsillitis, medium otitis and upper respiratory tract infection. The proportion of inadequacy, comparing the diagnostic hypothesis registered and the used antimicrobial, was higher than 25%. The inadequacy of the dose was more frequent in the pediatric. It has been found hard difficulties to find the medical records selected from the prescriptions, what required replacement of 73,3% of the sample. It has been also observed a systematic absence of general information as age, weight, presence of co-morbidity and reports of adverse reactions. Data analysis showed the need for review some stages of the work process and take measures that allow an appropriate accompaniment of the patients by the health team. It was also possible to verify, the need of implementation of rules that makes possible controlling the prescription and the use of antimicrobial agent, assuring the rational use of this specific medication by the municipal health services. Key words: antimicrobial, prescription, medical records, and primary health care.
A utilização de antimicrobianos tem suscitado discussões acerca da racionalidade de sua utilização com vistas a prevenir o surgimento de cepas de microorganismos resistentes. Desta forma, este trabalho teve como objetivo investigar a utilização de antibacterianos de uso sistêmico em unidades de atenção primária da Gerência de Saúde Centro Sul da Secretaria Municipal de Saúde de Belo Horizonte. Trata-se de um estudo observacional, transversal, que constou de duas etapas, sendo que na primeira, foi realizada uma avaliação das prescrições aviadas durante o mês março de 2002, utilizando-se indicadores da Organização Mundial de Saúde e na segunda, foi feita uma avaliação de prontuários médicos correspondentes a uma amostra das receitas. Constatou-se que cerca de 20% das receitas aviadas continham pelo menos um antibacteriano de uso sistêmico e destas, aproximadamente 46% originaram-se em unidades de saúde não pertencentes à Secretaria Municipal de Saúde de Belo Horizonte. A pediatria apresentou o maior índice de prescrição de antimicrobianos. A amoxicilina foi o antibacteriano mais prescrito, seguido pela benzilpenicilina benzatina, a associação sulfametoxazol+trimetoprim e a eritromicina. Observou-se índices de presença de dados técnicos (dose, intervalo entre doses, via de administração e tempo de tratamento) nas prescrições variando de 23,6 a 99,6%. Em cerca de 10% das receitas, não havia definição do tempo de tratamento para os antimicrobianos. A amoxicilina foi o antibacteriano que apresentou os melhores resultados em relação aos indicadores da prescrição e a eritromicina os piores. Em relação aos prontuários, as hipóteses diagnósticas registradas com maior freqüência foram tonsilite, otite média e infecções das vias aéreas superiores inespecíficas. O percentual de inadequação entre a hipótese diagnóstica registrada e o antimicrobiano utilizado, foi superior a 25%. A inadequação da dose foi mais freqüente na pediatria. Encontrou-se considerável dificuldade na localização dos prontuários selecionados a partir das receitas, exigindo substituição de 73,3% da amostra. Observou-se uma ausência sistemática de registro de informações gerais no prontuário como idade, peso, presença de co-morbidades e relato de reações adversas. Concluiu-se que há necessidade da revisão de algumas etapas do processo de trabalho e adoção de medidas que permitam um adequado acompanhamento dos pacientes pela equipe multiprofissional. Pôde-se observar também, a necessidade de implementação de medidas que visem controlar a prescrição e utilização de antimicrobianos, assegurando o uso racional desta classe de medicamentos na rede municipal de saúde. Palavras-chave: antimicrobianos, prescrição, prontuário, atenção primária.
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Piwowarski, Patrick [Verfasser], Franz [Gutachter] Bartl, Peter [Gutachter] Hildebrandt, and Peter [Gutachter] Hegemann. "FTIR-spektroskopische Untersuchungen am Phytochrom Agp2 / Patrick Piwowarski ; Gutachter: Franz Bartl, Peter Hildebrandt, Peter Hegemann." Berlin : Lebenswissenschaftliche Fakultät, 2017. http://d-nb.info/1135242003/34.

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Ali, Hazem Abd El-Rahman Obiadalla. "Understanding of carbon partitioning in tomato fruit." Doctoral thesis, Humboldt-Universität zu Berlin, Landwirtschaftlich-Gärtnerische Fakultät, 2003. http://dx.doi.org/10.18452/14900.

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Während der Entwicklung von Früchten der Tomate (Sorte Micro-Tom) wurde der Kohlenhydrat-Stoffwechsel untersucht. Es wurde ein Unterschied zwischen dem Metabolismus im Perikarp und dem des Plazenta-Gewebes gefunden. Stärke wurde in der Plazenta langsamer abgebaut als im Perikarp, während lösliche Zucker im Perikarp stärker akkumulierten. Die Aktivitäten der glykolytischen Enzyme tendierten zu einem Maximum 40 Tage nach der Blüte. Weiterhin wurde die Expression einiger plastidärer Transporter untersucht. Sowohl der Triosephosphat-Tranporter (TPT) als auch der Glucose-6-phosphat-Transporter wurden am stärksten in grünen Früchten exprimiert, während der Reife nahm die Expression ab. Der ATP/ADP-Transporter wurde während der Fruchtentwicklung nur schwach exprimiert.Es besteht die Hypothese, daß die Rolle der drei Enzyme plastidäre Fructose-1,6-Bisphosphatase (cp-FBPase), ADP-Glucose Pyrophosphorylase (AGPase) und Glucan Wasser Dikinase (GWD) darin besteht, die Stärke-Akkumulation in der frühen Entwicklung der Tomaten-Frucht zu beeinflussen. Diese Hypothese wurde unter Verwendung der Antisense-Technik für die plastidären FBPase (unter der Kontrolle des B33 Promoters), sowie für die AGPase und die GWD (beide unter der Kontrolle des CaMV 35S-Promoters) in der Tomaten-Kultivar Moneymaker untersucht. Die Repression von plastidärer FBPase oder AGPase in der Frucht der Tomate scheint die Metaboliten-Konzentrationen nicht so stark wie in den Blättern zu beeinflussen. Der Grund hierfür ist wahrscheinlich, daß jede Veränderung durch die Fähigkeit der Frucht, Zucker zu importieren, abgepuffert wird. Auf der anderen Seite hatte die Repression des GWD Proteins in der Frucht der Tomate starke Effekte auf die Metaboliten-Konzentrationen.
Carbohydrate metabolism was studied during the development of fruits of the tomato cultivar Micro-Tom. The metabolism of the pericarp and placental tissues was found to be different. Starch being degraded more slowly in the placenta than in the pericarp, while soluble sugars accumulated to a greater extent in the pericarp. The activities of glycolytic enzymes tended to peak at 40 days after flowering. The expression of some plastidial transporters was also studied. Both the triose phosphate transporter (TPT) and Glucose-6-Phosphate (Glc-6-P) transporter were expressed greatest in green fruits, before declining. The expression of the triose phosphate transporter (TPT) was greater than that of Glc-6-P transporter. The ATP/ADP transporter was expressed to a low level throughout fruit development. The role of three enzymes Chloroplastic Fructose-1,6-bisphosphatase (cp-FBPase), ADP-glucose Pyrophosphorylase (AGPase) and Glucan Water Dikinase (GWD) protein are thought to influence the accumulation of starch in early development in tomato fruit were studied in normal sized tomatoes of the cultivar Moneymaker using antisense technique under the control of the patatin B33 promoter in the case of cp-FBPase, and the CaMV 35S promoter in the case of AGPase and GWD protein. It appears that repression of cp-FBPase and AGPase in tomato fruits does not influence metabolite levels as greatly as it does in leaves, possibly because any alterations are buffered by the ability of the fruit to import sugars. On the other hand, the repression of GWD protein in tomato fruits has a strong effect on metabolite levels.
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Books on the topic "Agpat2"

1

Conde, Teresa del. Agpa 2000. Edited by Horz de Vía Elena, Colina Armando, Torre Mario de la, and Smurfit Cartón y Papel de México (Firm). Smurfit Cartón y Papel de México, 2000.

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San Pedro Museo de Arte and Smurfit Cartón y Papel de México (Firm), eds. Gráfica, arte en transformación: Colección AGPA : San Pedro Museo de Arte, octubre 2006-febrero 2007. Smurfit Kappa, 2007.

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Agpa Membership Directory, 1994. 3rd ed. Amer Medical Group Assn, 1994.

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Association, American Group Practice. Agpa Membership Directory 1997 (32nd). 3rd ed. Amer Medical Group Assn, 1997.

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Agpa Membership Directory 1995 (31st ed). 3rd ed. Amer Medical Group Assn, 1995.

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Book chapters on the topic "Agpat2"

1

Ramanathan, N., M. Ahmed, E. Raffan, et al. "Identification and Characterisation of a Novel Pathogenic Mutation in the Human Lipodystrophy Gene AGPAT2." In JIMD Reports. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/8904_2012_181.

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Ramanathan, N., M. Ahmed, E. Raffan, et al. "Erratum to: Identification and Characterisation of a Novel Pathogenic Mutation in the Human Lipodystrophy Gene AGPAT2." In JIMD Reports. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-35518-9_196.

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Villars, P., K. Cenzual, J. Daams, et al. "AgPO2(NH2)2." In Structure Types. Part 8: Space Groups (156) P3m1 – (148) R-3. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-70892-6_229.

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Jain, M., and A. Gupta. "1663 Diamagnetic susceptibility of AgP2." In Diamagnetic Susceptibility and Anisotropy of Inorganic and Organometallic Compounds. Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-44694-1_1664.

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Jain, M., and A. Gupta. "2397 Diamagnetic susceptibility of AgP2." In Diamagnetic Susceptibility and Anisotropy of Inorganic and Organometallic Compounds. Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-44694-1_2398.

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Leszcz, Molyn, and Joseph C. Kobos. "Wie wissenschaftliche Evidenz praktisch genutzt werden kann: Gruppenpsychotherapie und die »Leitlinien für die klinische Praxis« der American Group Psychotherapy Association (AGPA)." In Gruppenpsychotherapie. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-03497-8_17.

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Leszcz, Molyn, and Joseph C. Kobos. "Wie wissenschaftliche Evidenz praktisch genutzt werden kann: Gruppenpsychotherapie und die »Leitlinien für die klinische Praxis« der American Group Psychotherapy Association (AGPA)." In Gruppenpsychotherapie. Springer Berlin Heidelberg, 2017. http://dx.doi.org/10.1007/978-3-662-54644-4_17.

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"agpaite." In Dictionary Geotechnical Engineering/Wörterbuch GeoTechnik. Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-41714-6_11005.

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Ahmed, M., H. Raef, E. Raffan, and R. K. Semple. "Severe Insulin Resistance, Diabetes Mellitus, Hypertriglycridemia, and Pseudoacromegaly Due to Novel Homozygous Mutation in the Acyl-3-Phospate O-Acyltransferase 2, (AGPAT2) Gene." In The Endocrine Society's 92nd Annual Meeting, June 19–22, 2010 - San Diego. Endocrine Society, 2010. http://dx.doi.org/10.1210/endo-meetings.2010.part3.p11.p3-548.

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Horwitz, Leonard. "Exciting opportunities ahead: opening plenary address to AGPA institute participants." In Listening with the Fourth Ear. Routledge, 2018. http://dx.doi.org/10.4324/9780429476716-23.

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Conference papers on the topic "Agpat2"

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Budi, Setia, Lintang Dhanasmoro, B. Asih Suliasih, Ucu Cahyana, Agung Purwanto, and Sukro Muhab. "Electrodeposition of nanobranched AgPt for methylene blue photodegradation." In THE 2ND SCIENCE AND MATHEMATICS INTERNATIONAL CONFERENCE (SMIC 2020): Transforming Research and Education of Science and Mathematics in the Digital Age. AIP Publishing, 2021. http://dx.doi.org/10.1063/5.0041637.

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Fentaye, Amare, Valentina Zaccaria, Moksadur Rahman, Mikael Stenfelt, and Konstantinos Kyprianidis. "Hybrid Model-Based and Data-Driven Diagnostic Algorithm for Gas Turbine Engines." In ASME Turbo Expo 2020: Turbomachinery Technical Conference and Exposition. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/gt2020-14481.

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Abstract Data-driven algorithms require large and comprehensive training samples in order to provide reliable diagnostic solutions. However, in many gas turbine applications, it is hard to find fault data due to proprietary and liability issues. Operational data samples obtained from end-users through collaboration projects do not represent fault conditions sufficiently and are not labeled either. Conversely, model-based methods have some accuracy deficiencies due to measurement uncertainty and model smearing effects when the number of gas path components to be assessed is large. The present paper integrates physics-based and data-driven approaches aiming to overcome this limitation. In the proposed method, an adaptive gas path analysis (AGPA) is used to correct measurement data against the ambient condition variations and normalize. Fault signatures drawn from the AGPA are used to assess the health status of the case engine through a Bayesian network (BN) based fault diagnostic algorithm. The performance of the proposed technique is evaluated based on five different gas path component faults of a three-shaft turbofan engine, namely intermediate-pressure compressor fouling (IPCF), high-pressure compressor fouling (HPCF), high-pressure turbine erosion (HPTE), intermediate-pressure turbine erosion (IPTE), and low-pressure turbine erosion (LPTE). Robustness of the method under measurement uncertainty has also been tested using noise-contaminated data. Moreover, the fault diagnostic effectiveness of the BN algorithm on different number and type of measurements is also examined based on three different sensor groups. The test results verify the effectiveness of the proposed method to diagnose single gas path component faults correctly even under a significant noise level and different instrumentation suites. This enables to accommodate measurement suite inconsistencies between engines of the same type. The proposed method can further be used to support the gas turbine maintenance decision-making process when coupled with overall Engine Health Management (EHM) systems.
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