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Dissertations / Theses on the topic 'Agrobacterium tumefaciens-mediated transformation of fungi'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Knight, Claire Jane. "Investigation into agrobacterium-mediated transformation of fungi in nauture." Thesis, University of Bristol, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500434.

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2

Zemska, Olga. "Discovery Of Intracellular Growth Requirements of the Fungal Pathogen Histoplasma capsulatum." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1343803044.

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3

Meyer, Tania. "Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum f. sp. cubense for pathogenicity gene analysis." Pretoria : [s.n.], 2009. http://upetd.up.ac.za/thesis/available/etd-06122009-132700/.

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4

Meyer, Tanja. "Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum f. sp. cubense for pathogenicity gene analysis." Diss., University of Pretoria, 2008. http://hdl.handle.net/2263/25473.

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Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), is one of the most destructive plant diseases in recorded history. The disease was first discovered in Australia in 1874 but became renowned for the severe losses it caused to export banana plantations during the 1960s in Central America. The banana export industry was saved only by replacing Gros Michel bananas, the dessert banana grown for the export market, with highly resistant Cavendish banana cultivars. Despite this apparent solution, the fungus was found to attack Cavendish bananas in the sub-tropics, where plan

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5

Celikkol, Akcay Ufuk. "Regeneration Of Lentil (lens Culinaris Medik) And Genetic Transformation By Using Agrobacterium Tumefaciens-mediated Gene Transfer." Phd thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/3/12609425/index.pdf.

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In this study, the effects of different plant growth regulators on regeneration responses of various lentil explants through direct and indirect organogenesis and through somatic embryogenesis from calli and cell suspension cultures were investigated. Shoot regeneration was obtained in low frequencies from longitudinal embryonic axis explants and nodal buds of epicotyls, however whole plant regeneration was unsuccessful. Conditions provided for indirect organogenesis resulted only in swelling of hypocotyls and root directed ends of internodes and weak callus formation on leaves which were foll

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6

Dube, Thabani. "The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation." Doctoral thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/4255.

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Bibliography: leaves 165-195.<br>The transfer of T-DNA from Agrobacterium tumefaciens to eukaryotic cells is on of the most extensively studied examples of interkingdom DNA transfer. This transfer system has revolutionized plant molecular biology to the extent that the introduction of foreign genes into plant genomes is now a basic technique. Apart from the finding that a defined segment (T-DNA) of the A. tumefaciens Ti plasmid can be transferred to plant DNA, the discovery that the mobilization functions of the IncQ plasmid, RSF1010, can mediate its transfer from A. tumefaciens into plant ce

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7

Akpadock, Evelyn. "Analysis of a ufdB Penicillium marneffei Mutant Generated by Agrobacterium tumefaciens-Mediated Transformation." Youngstown State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1377083669.

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8

Demirbas, Didem. "Optimization Of Regeneration And Agrobacterium Mediated Transformation Of Wheat (triticum Aestivum L.cv. Yuregir 89)." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/3/12605454/index.pdf.

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The objective of this study was to optimize regeneration parameters of immature inflorescence culture of Triticum aestivum cv. Y&uuml<br>regir-89. The effects of dark incubation period and explant region on regeneration success were tested. Immature inflorescences were cut into 3 pieces as tip, mid, base and put onto 2mg /L 2,4-dichlorophenoxyacetic acid containing callus induction medium. These explants were taken to regeneration after 6, 9, 13 weeks of dark incubation period. The regeneration capacities of calli were determined as rooting and shooting percentages. Shooting percentages were f

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9

Baloglu, Cengiz Mehmet. "Optimization Of Regeneration And Agrobacterium Mediated Transformation Of Sugar Beet (beta Vulgaris L.)." Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/3/12606476/index.pdf.

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In this study, optimization of a transformation and regeneration system via indirect and direct organogenesis in cotyledon, hypocotyl, petiole, leaf and shoot base tissues of sugar beet (Beta vulgaris L. cv. ELK 345 and 1195) was investigated. Two different germination, three different callus induction and shoot induction medium was used for indirect organogenesis of sugar beet cultivar ELK 345. Except cotyledon, other explants (hypocotyl, petiole and leaf) produced callus. However no shoot development was observed from callus of these explants. Shoot base tissue of sugar beet cultivar 1195 wa

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10

Oz, M. Tufan. "Optimization Of Selection Conditions And Agrobacterium Mediated Transformation Of Chickpea (cicer Arietinum L. Cv. Gokce)." Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/3/12605842/index.pdf.

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The objective of this study was to optimize an efficient selection system and Agrobacterium mediated transformation of chickpea (Cicer arietinum L.). Cotyledonary node explants of Turkish chickpea cultivar G&ouml<br>k&ccedil<br>e were used to determine the effects of selective agents, two antibiotics (Kanamycin, Hygromycin) and two herbicides (PPT, Glyphosate) as well as four antibiotics (Augmentin, Carbenicillin, Cefotaxime, Timentin) for eliminating Agrobacterium on multiple shoot and root induction. Selective agents and antibiotics were applied to explants at different concentrations for

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11

Hutchinson, Chad M. "Agrobacterium tumefaciens mediated transformation of orchid tissue with the sense and antisense coat protein genes from the odontoglossum ringspot virus." Virtual Press, 1992. http://liblink.bsu.edu/uhtbin/catkey/834608.

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This research was an attempt to use a dicot transformation vector to transform a monocot. The initial purpose of this thesis was to transform orchids with the sense and antisense coat protein genes from the Odontoglossum ringspot virus (ORSV) in an effort to mitigate viral symptoms in transgenic plants using the transformation vector, Agrobacterium tumefaciens. However, it soon became apparent that much time would be needed to develop a transformation protocol. The transformation vectors used included the Agrobacterium tumefaciens disarmed strain LBA4404 with the binary plasmid pB1121, the dis

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12

Mohd, Fazli Bin Farida Asras. "Molecular breeding and biochemical characterization of an oleaginous fungus Mortierella alpina for prostaglandin F2α production". Kyoto University, 2019. http://hdl.handle.net/2433/242698.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第21821号<br>農博第2334号<br>新制||農||1067(附属図書館)<br>学位論文||H31||N5193(農学部図書室)<br>京都大学大学院農学研究科応用生命科学専攻<br>(主査)教授小川順, 教授植田充美, 教授栗原達夫<br>学位規則第4条第1項該当

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13

Ferreira, Vanessa Agostinho. "Unravelling Lasiodiplodia theobromae and Lasiodiplodia hormozganensis virulence via functional genomics and metabolites isolation." Master's thesis, 2019. http://hdl.handle.net/10773/28314.

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Global climate change may lead to interactions of pathogenic fungi with new hosts, even from different kingdoms. In order for this to occur, fungi need to develop specific virulence mechanisms for the new host, for example through production of virulence factors. Some phytopathogenic fungi belonging to Botryosphaeriaceae are known human opportunistic pathogens. Moreover, the genus Lasiodiplodia has been reported in several human infections. Nonetheless, the virulence mechanisms behind this ability are still in need of research. The goal of this thesis consisted on the study of virulence toward

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14

Su, Mei-Hsiu, and 蘇湄琇. "Agrobacterium tumefaciens mediated transformation of horned melon (Cucumis metuliferus)." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/10133505524774726954.

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碩士<br>國立中興大學<br>農藝學系所<br>97<br>Papaya ringspots virus (PRSV) and type W strain of papaya ringspot spot (PRSV-W) are limiting yields of melon and cucumber. The horned melon was minor crop, and the accession (P. I. 292190) of horned melon was resistant PRSV and PRSV-W by a single completely dominant gene. In this study, we tried to establish a model system for introduction of foreign genes into horned melon plant by Agrobacterium-mediated transformation. We used NPTII and GUS gene as a selection marker and reporter gene. In this study, the cotyledon explants was evaluated on various combinatio

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15

CHEN, KUN-LONG, and 陳坤龍. "Transformation of tomato (Lycopersicon esculentum L.) mediated by agrobacterium tumefaciens." Thesis, 1991. http://ndltd.ncl.edu.tw/handle/20194808796967324462.

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16

Chen, Chia-Pei, and 陳佳珮. "Study on the Agrobacterium tumefaciens-mediated transformation of Hypsizygus marmoreus variant." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/10305873923906659744.

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碩士<br>國立臺灣大學<br>微生物與生化學研究所<br>97<br>The molecular farming may produce pharmaceutically valuable protein or enzymes of the industrial application. The establishment of gene transformation contribute to develop molecular breeding and molecular farming. In this study, the expression system using Agrobacterium tumefaciens-mediated transformation (ATMT) was developed. A. tumefaciens inserts the T-DNA which is a portion of Ti-plasmid into the genome DNA randomly during infecting host. It could make the target gene more stable and enhance protein analysis. The expression plasmids were constructed

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17

Lin, Yung-Chu, and 林詠筑. "Identification of pathogenicity genes of Colletotrichum acutatum with Agrobacterium tumefaciens mediated transformation." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/ysgq4h.

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碩士<br>國立中興大學<br>植物病理學系所<br>102<br>Chili pepper (Capsicum spp.) is an important spice in the world. Anthracnose, which causes by Colletotrichum species, is one of the most important fungal diseases on chili pepper and can reduce yield marketablely. All growth stages of chili pepper fruit could be affected by anthracnose. The typical symptom on chili pepper fruit is sunken necrotic tissues with concentric rings of acervuli. In Taiwan, Colletotrichum acutatum is the major species causing chili anthracnose, resulting in severe yield losses. Agrobacterium tumefaciens-mediated transformation (ATMT)

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18

Cheng, Chih-Jui, and 程致瑞. "Enhancement of protein expression in Hypsizygus marumoreus by multiple Agrobacterium tumefaciens-mediated transformation." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/21243337675955965941.

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碩士<br>國立臺灣大學<br>生化科技學系<br>100<br>Mushroom molecular farming recently attracts tremendous attention because of its application pontentials and the advantages over plant molecular farming. Agrobacterium tumefaciens-mediated transformation (ATMT) is commonly used in mushroom transformation but its application was limited due to the low heterologous gene expression. In this study, Hypsizygus marumoreus was chosen as the expression host and was transformed by multiple ATMT in order to enhance heterologous protein expression. For the first step of multiple ATMT, A. tumefaciens harboring p0390-Cbx-Hi

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19

Tsai, Yuan-Chen, and 蔡媛禎. "Agrobacterium tumefaciens mediated transformation as a tool for heterologous expression in Pleurotus ostreatus." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/40160858360021075188.

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碩士<br>國立臺灣大學<br>微生物與生化學研究所<br>97<br>Pleurotus ostreatus is the second largest edible mushrooms next to Agaricus bisporus. P. ostreatus is popular worldwide due to ease of and use of agricultural wastes as substrates. Establishing a stable transformation system of P. ostreatus may provide a tool for its heterologous expression and even oral vaccine production in the future. Agrobacterium tumefaciens has been known as “natural engineer”. There are many advantages of Agrobacterium tumefaciens mediated transformation (ATMT) over other transformation methods such as convenience and less expenssive.

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20

Hsu, Yun-Han, and 徐韻涵. "Study on the expression system of Flammulina velutipes by Agrobacterium tumefaciens-mediated transformation." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/14699739239879070804.

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碩士<br>國立臺灣大學<br>微生物與生化學研究所<br>96<br>Molecular farming is the production of pharmaceutically important and industrially valuable proteins or enzymes in plants and mushrooms. Research in the past two decades has developed heterologous protein expression in yeasts and filamentous fungi. However, the lack of an efficient and stable transformation system in filamentous fungi is one of the major problems for recombinant protein production in mushrooms. In this study, the expression system of Flammulina velutipes using Agrobacterium tumefaciens-mediated transformation (ATMT) was developed. A. tumefac

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21

Naveen, K. S. "Studies on Agrobacterium tumefaciens-mediated genetic transformation in some important Indian selections of coffee." Thesis, 2002. http://hdl.handle.net/2009/1431.

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22

Rush, Jason L. "Insertion of the enzyme cyclopropane fatty acid synthase into plastids through Agrobacterium mediated transformation /." 2008. http://digitalcommons.butler.edu/ugtheses/57/.

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23

Brown, Jonathan Warren. "Agrobacterium tumefaciens mediated transformation of sweet potato ( Ipomoea batatas) tuber and regeneration of transformed tissue." Thesis, 2016. http://hdl.handle.net/10539/21516.

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A research report submitted to the Faculty of Science, University of the Witwatersrand, in partial fulfilment of the requirements for the degree of Master of Science, Johannesburg, February 1998.<br>Sweet potato (Ipomoea batatasy is one of the six biggest crops in the world, its high nutritional content and large yield in tropical areas making it a useful food source, especially in developing countries. Genetic engineering has the ability to overcome factors such as insect and disease damage which are currently limiting its potential. With this in mind research has been conducted into th

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24

Li, Wen-Yi, and 李玟儀. "Studies of pathogenicity or virulence related mechenisms of Fusarium oxysporum f. sp. niveum by Agrobacterium tumefaciens-mediated transformation method." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/13253910680067229821.

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