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Journal articles on the topic 'Aida'

Author: Grafiati

Published: 4 June 2021

Last updated: 29 May 2022

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1

Charbonneau, Marie-Ève, Frédéric Berthiaume, and Michael Mourez. "Proteolytic Processing Is Not Essential for Multiple Functions of the Escherichia coli Autotransporter Adhesin Involved in Diffuse Adherence (AIDA-I)." Journal of Bacteriology 188, no. 24 (October 13, 2006): 8504–12. http://dx.doi.org/10.1128/jb.00864-06.

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ABSTRACT The Escherichia coli adhesin involved in diffuse adherence (AIDA-I), like many other autotransporter proteins, is released in the periplasm as a proprotein undergoing proteolytic processing after its translocation across the outer membrane. The proprotein is cleaved into a membrane-embedded fragment, AIDAc, and an extracellular fragment, the mature AIDA-I adhesin. The latter remains noncovalently associated with the outer membrane and can be released by heat treatment. The mechanism of cleavage of the proprotein and its role in the functionality of AIDA-I are not understood. Here, we show that cleavage is independent of the amount of AIDA-I in the outer membrane, suggesting an intramolecular autoproteolytic mechanism or a cleavage mediated by an unknown protease. We show that the two fragments, mature AIDA-I and AIDAc, can be cosolubilized and copurified in a folded and active conformation. We observed that the release by heat treatment results from the unfolding of AIDA-I and that the interaction of AIDA-I with AIDAc seems to be disturbed only by denaturation. We constructed an uncleavable point mutant of AIDA-I, where a serine of the cleavage site was changed into a leucine, and showed that adhesion, autoaggregation, and biofilm formation mediated by the mutant are indistinguishable from the wild-type levels. Lastly, we show that both proteins can mediate the invasion of cultured epithelial cells. Taken together, our experiments suggest that the proteolytic processing of AIDA-I plays a minor role in the functionality of this protein.

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2

Sullivan, Gary, and Kenneth Fordyce. "Artificial intelligence development aids (AIDA)." ACM SIGAPL APL Quote Quad 15, no. 4 (May 12, 1985): 106–13. http://dx.doi.org/10.1145/255315.255347.

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3

Laarmann, Sven, and M. Alexander Schmidt. "The Escherichia coli AIDA autotransporter adhesin recognizes an integral membrane glycoprotein as receptor." Microbiology 149, no. 7 (July 1, 2003): 1871–82. http://dx.doi.org/10.1099/mic.0.26264-0.

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The AIDA-I autotransporter adhesin, as a prototype of the AIDA adhesin family, represents a tripartite antigen consisting of the functional adhesin AIDA-I (α-domain), which mediates the specific attachment of bacteria to target cells, and a two-domain translocator (AIDAc) organized in the β 1- and β 2-domains. Cellular receptor moieties for the adhesin AIDA-I have not been identified. Here, it is demonstrated that the purified adhesin binds specifically to a high-affinity class of receptors on HeLa cells. Additionally, the adhesin was found to bind to a variety of mammalian cell types, indicating a broad tissue distribution of the receptor moiety. By using complementary techniques, including co-immunoprecipitation and one- and two-dimensional gel electrophoresis, the AIDA-I binding protein on HeLa cells was identified as a surface glycoprotein of about 119 kDa (gp119). The gp119 AIDA-I cellular receptor protein was characterized biochemically and found to be an integral N-glycosylated membrane protein with a pI of 5·2.

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4

He, Tian, Brian M. Blum, John A. Stankovic, and Tarek Abdelzaher. "AIDA." ACM Transactions on Embedded Computing Systems 3, no. 2 (May 2004): 426–57. http://dx.doi.org/10.1145/993396.993406.

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5

D'silva, Joseph Vinish, Florestan De Moor, and Bettina Kemme. "AIDA." Proceedings of the VLDB Endowment 11, no. 11 (July 2018): 1400–1413. http://dx.doi.org/10.14778/3236187.3236194.

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6

Yosef, Mohamed Amir, Johannes Hoffart, Ilaria Bordino, Marc Spaniol, and Gerhard Weikum. "AIDA." Proceedings of the VLDB Endowment 4, no. 12 (August 2011): 1450–53. http://dx.doi.org/10.14778/3402755.3402793.

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7

Parker, C. P. Gerald. "Aida." Opera Quarterly 5, no. 2-3 (1987): 238–44. http://dx.doi.org/10.1093/oq/5.2-3.238.

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8

Muthmainnah, Ani, Ely Triasih Rahayu, and Hartati Hartati. "Perbedaan Penggunaan Setsuzokushi Aida dan Aida ni." J-Litera: Jurnal Kajian Bahasa, Sastra dan Budaya Jepang 2, no. 1 (May 29, 2020): 36. http://dx.doi.org/10.20884/1.jlitera.2020.2.1.2115.

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The research is entitled The Differences between Setsuzokushi Aida and Aida ni. The aims of this research are to describe the use of sentence structure using setsuzokushi aida and aida ni in Japanese, and to describe the differences of setsuzokushi aida and aida ni usage. This research belongs to descriptive qualitative and the data collection uses library techniques. In total, the data from this research are 19 sentences containing setsuzokushi aida and aida ni taken from Minna no Nihongo Shokyuu I&II, Chukyuu I and Indoneshiago Bunreishuu Nihongo-Indoneshiago (Doushihen) books. Based on the results of the analysis, it can be concluded that setsuzokushi aida is a conjunction that describes two activities carried out in the same period of time, continuously and finished simultaneously, whereas setsuzokushi aida ni is a conjunction that explains two activities carried out in the different periods of time and momentarily.

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9

Eden, Bradford Lee. "Aida (review)." Notes 62, no. 3 (2006): 782. http://dx.doi.org/10.1353/not.2006.0013.

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10

Rathod, Prof Arvind. "AIDA model of Advertising Strategy." Indian Journal of Applied Research 1, no. 10 (October 1, 2011): 122–25. http://dx.doi.org/10.15373/2249555x/jul2012/39.

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11

Sherlock, Orla, Mark A. Schembri, Andreas Reisner, and Per Klemm. "Novel Roles for the AIDA Adhesin from Diarrheagenic Escherichia coli: Cell Aggregation and Biofilm Formation." Journal of Bacteriology 186, no. 23 (December 1, 2004): 8058–65. http://dx.doi.org/10.1128/jb.186.23.8058-8065.2004.

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ABSTRACT Diarrhea-causing Escherichia coli strains are responsible for numerous cases of gastrointestinal disease and constitute a serious health problem throughout the world. The ability to recognize and attach to host intestinal surfaces is an essential step in the pathogenesis of such strains. AIDA is a potent bacterial adhesin associated with some diarrheagenic E. coli strains. AIDA mediates bacterial attachment to a broad variety of human and other mammalian cells. It is a surface-displayed autotransporter protein and belongs to the selected group of bacterial glycoproteins; only the glycosylated form binds to mammalian cells. Here, we show that AIDA possesses self-association characteristics and can mediate autoaggregation of E. coli cells. We demonstrate that intercellular AIDA-AIDA interaction is responsible for bacterial autoaggregation. Interestingly, AIDA-expressing cells can interact with antigen 43 (Ag43)-expressing cells, which is indicative of an intercellular AIDA-Ag43 interaction. Additionally, AIDA expression dramatically enhances biofilm formation by E. coli on abiotic surfaces in flow chambers.

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12

Charbonneau, Marie-Ève, Victoria Girard, Anastasia Nikolakakis, Manuel Campos, Frédéric Berthiaume, France Dumas, François Lépine, and Michael Mourez. "O-Linked Glycosylation Ensures the Normal Conformation of the Autotransporter Adhesin Involved in Diffuse Adherence." Journal of Bacteriology 189, no. 24 (October 19, 2007): 8880–89. http://dx.doi.org/10.1128/jb.00969-07.

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ABSTRACT The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is one of the few glycosylated proteins found in Escherichia coli. Glycosylation is mediated by a specific heptosyltransferase encoded by the aah gene, but little is known about the role of this modification and the mechanism involved. In this study, we identified several peptides of AIDA-I modified by the addition of heptoses by use of mass spectrometry and N-terminal sequencing of proteolytic fragments of AIDA-I. One threonine and 15 serine residues were identified as bearing heptoses, thus demonstrating for the first time that AIDA-I is O-glycosylated. We observed that unglycosylated AIDA-I is expressed in smaller amounts than its glycosylated counterpart and shows extensive signs of degradation upon heat extraction. We also observed that unglycosylated AIDA-I is more sensitive to proteases and induces important extracytoplasmic stress. Lastly, as was previously shown, we noted that glycosylation is required for AIDA-I to mediate adhesion to cultured epithelial cells, but purified mature AIDA-I fused to GST was found to bind in vitro to cells whether or not it was glycosylated. Taken together, our results suggest that glycosylation is required to ensure a normal conformation of AIDA-I and may be only indirectly necessary for its cell-binding function.

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13

Baxter, R. "Aida. Giuseppe Verdi." Opera Quarterly 22, no. 1 (December 13, 2006): 176–79. http://dx.doi.org/10.1093/oq/kbi081.

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14

Burroughs, Bruce. "Aida Giuseppe Verdi." Opera Quarterly 8, no. 4 (1991): 115–21. http://dx.doi.org/10.1093/oq/8.4.115.

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15

Rishoi, N. "Aida. Giuseppe Verdi." Opera Quarterly 17, no. 1 (January 1, 2001): 115–17. http://dx.doi.org/10.1093/oq/17.1.115.

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16

McKee, D. "Aida. Giuseppe Verdi." Opera Quarterly 17, no. 2 (January 1, 2001): 331–34. http://dx.doi.org/10.1093/oq/17.2.331.

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17

Pines, R. "Aida. Giuseppe Verdi." Opera Quarterly 19, no. 2 (April 1, 2003): 293–96. http://dx.doi.org/10.1093/oq/19.2.293.

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18

Jellinek, George. "Aida. Giuseppe Verdi." Opera Quarterly 8, no. 1 (1991): 129–30. http://dx.doi.org/10.1093/oq/8.1.129.

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19

Mazzanti, F. "The AIDA experiment." ACM SIGAda Ada Letters IX, no. 5 (July 1989): 109–14. http://dx.doi.org/10.1145/71340.71347.

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20

Locke, Ralph P. "David and Aida." Musical Times 129, no. 1744 (June 1988): 282. http://dx.doi.org/10.2307/964878.

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21

Ha, Seung-Kwon, Changsun Choi, and Chanhee Chae. "Prevalence of a Gene Encoding Adhesin Involved in Diffuse Adherence among Escherichia Coli Isolates in Pigs with Postweaning Diarrhea or Edema Disease." Journal of Veterinary Diagnostic Investigation 15, no. 4 (July 2003): 378–81. http://dx.doi.org/10.1177/104063870301500414.

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A total of 604 Escherichia coli strains isolated from weaned pigs with diarrhea or edema disease on 653 swine farms were screened for the presence of the adhesin involved in diffuse adherence (AIDA) gene by polymerase chain reaction (PCR). Escherichia coli isolates that carried AIDA genes were also tested by PCR for the detection of 5 fimbriae (F4, F5, F6, F18, and F41), 3 heat-stable (STa, STb, and EAST1) and 1 heat-labile (LT) enterotoxin, and Shiga toxin 2e (Stx2e) genes. Forty-five (7.5%) of the 604 E. coli isolates carried the gene for AIDA. Of these 45 isolates, 5 (11.1%) carried EAST1 genes only, 1 (2.2%) carried genes for at least one of the fimbrial adhesins, 12 (26.7%) carried genes for at least one of the toxins, and 27 (60%) carried genes for at least one of the fimbrial adhesins and toxins. Fifty-one percent of strains that carried AIDA genes carried Stx2e genes, and 40% of strains that carried AIDA genes carried F18ab. The isolation rate of enterotoxigenic E. coli strain carrying genes for AIDA was 87%, and the isolation rate of Shiga toxin-producing E. coli strain carrying genes for AIDA was 49%. AIDA may represent an important virulence determinant in pigs with postweaning diarrhea or edema disease.

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22

Lehmann, Eldon D., Dennis K. DeWolf, Christopher A. Novotny, Karen Reed, and Robert R. Gotwals. "Dynamic Interactive Educational Diabetes Simulations Using the World Wide Web: An Experience of More Than 15 Years with AIDA Online." International Journal of Endocrinology 2014 (2014): 1–25. http://dx.doi.org/10.1155/2014/692893.

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Background. AIDA is a widely available downloadable educational simulator of glucose-insulin interaction in diabetes.Methods. A web-based version of AIDA was developed that utilises a server-based architecture with HTML FORM commands to submit numerical data from a web-browser client to a remote web server. AIDA online, located on a remote server, passes the received data through Perl scripts which interactively produce 24 hr insulin and glucose simulations.Results. AIDA online allows users to modify the insulin regimen and diet of 40 different prestored “virtual diabetic patients” on the internet or create new “patients” with user-generated regimens. Multiple simulations can be run, with graphical results viewed via a standard web-browser window. To date, over 637,500 diabetes simulations have been run at AIDA online, from all over the world.Conclusions. AIDA online’s functionality is similar to the downloadable AIDA program, but the mode of implementation and usage is different. An advantage to utilising a server-based application is the flexibility that can be offered. New modules can be added quickly to the online simulator. This has facilitated the development of refinements to AIDA online, which have instantaneously become available around the world, with no further local downloads or installations being required.

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23

Moormann, Corinna, Inga Benz, and M. Alexander Schmidt. "Functional Substitution of the TibC Protein of Enterotoxigenic Escherichia coli Strains for the Autotransporter Adhesin Heptosyltransferase of the AIDA System." Infection and Immunity 70, no. 5 (May 2002): 2264–70. http://dx.doi.org/10.1128/iai.70.5.2264-2270.2002.

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ABSTRACT The plasmid-encoded AIDA (adhesin involved in diffuse adherence) autotransporter protein derived from diffuse-adhering clinical Escherichia coli isolate 2787 and the TibA (enterotoxigenic invasion locus B) protein encoded by the chromosomal tib locus of enterotoxigenic E. coli (ETEC) strain H10407 are posttranslationally modified by carbohydrate substituents. Analysis of the AIDA-I adhesin showed that the modification involved heptose residues. AIDA-I is modified by the heptosyltransferase activity of the product of the aah gene, which is located directly upstream of adhesin-encoding gene aidA. The carbohydrate modification of the TibA adhesin/invasin is mediated by the TibC protein but has not been elucidated. Based on the sequence similarities between TibC and AAH (autotransporter adhesin heptosyltransferase) and between the TibA and the AIDA proteins we hypothesized that the AIDA system and the Tib system encoded by the tib locus are structurally and functionally related. Here we show that (i) TibC proteins derived from different ETEC strains appear to be highly conserved, (ii) recombinant TibC proteins can substitute for the AAH heptosyltransferase in introducing the heptosyl modification to AIDA-I, (iii) this modification is functional in restoring the adhesive function of AIDA-I, (iv) a single amino acid substitution at position 358 completely abolishes this activity, and (v) antibodies directed at the functionally active AIDA-I recognize a protein resembling modified TibA in ETEC strains. In summary, we conclude that, like AAH, TibC represents an example of a novel class of heptosyltransferases specifically transferring heptose residues onto multiple sites of a protein backbone. A potential consensus sequence for the modification site is suggested.

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24

Lo Coco, Francesco, Giuseppe Avvisati, Marco Vignetti, Giuseppe Fioritoni, Vincenzo Liso, Felicetto Ferrara, Giuseppe Cimino, et al. "Front-Line Treatment of Acute Promyelocytic Leukemia with AIDA Induction Followed by Risk-Adapted Consolidation: Results of the AIDA-2000 Trial of the Italian GIMEMA Group." Blood 104, no. 11 (November 16, 2004): 392. http://dx.doi.org/10.1182/blood.v104.11.392.392.

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Abstract Following the identification, in collaboration with the Spanish PETHEMA group, of distinct prognostic categories among APL patients receiving AIDA-like therapies (Sanz et al. Blood 2000) the Italian GIMEMA group designed a protocol for newly diagnosed APL (AIDA-2000) in which the intensity of post-remission treatment was adapted to the relapse risk. A total of 298 PML/RARa-positive patients with median age 40 yrs (range 1–60) were enrolled during the period January 2000 – February 2003 from 64 Italian institutions. After the standard AIDA-0493 induction (Mandelli et al, Blood 1997), patients with low- and intermediate-risk received 3 anthracycline-based consolidation courses with idarubicin, mitoxantrone, and idarubicin as in the PETHEMA LPA-96 (Sanz et al. Blood 1999), whereas patients with high-risk disease (WBC > 10 x 109/L) received the same 3 anthracycline courses with the addition of cytarabine, etoposide and cytarabine plus 6-thioguanine during the first, second and third course, respectively, as in the original AIDA. In addition, distinct from those in the AIDA-0493, all patients enrolled in the AIDA-2000 received concomitant ATRA 45 mg/m2 for 15 d during each consolidation course. The results of the AIDA-2000 series were compared to those obtained in 346 consecutive patients (median age 36 yrs, range 1–60) enrolled in the AIDA-0493 study during the period May 1997 – May 2000. All patients in either studies who tested PCR-negative post-consolidation received ATRA maintenance for a total of two years. After induction, 323/338 (96%) and 276/294 (94%) evaluable patients achieved CR in the AIDA-0493 and AIDA-2000, respectively (P=0.34). Molecular remission was obtained after consolidation in 291/296 (98%) and 235/238 (99%) patients (P=0.69). With a median follow-up of 4.5 and 2.0 yrs in the two studies, the DFS at 2.0 yrs for patients in the AIDA-0493 and AIDA-2000 was 84% and 90%, respectively (P=0.09), whereas the CIR rate at 2.0 yrs was 14% and 5%, respectively (P=0.04). Five and 9 patients died in CR in the two series and were equally distributed among risk groups. By comparing separately the distinct risk groups in the AIDA-0493 and AIDA-2000, there was no significant difference in the CIR rate for low- (3% vs. 2%) and intermediate-risk (11% vs. 9%) groups, while a significantly higher CIR was observed in the AIDA-0493 for high-risk (29% vs. 2%, P=0.0004). In line with recent PETHEMA results, our data confirm that anthracycline-based consolidation is equally effective as cytarabine-containing regimens for patients with low- and intermediate-risk and suggest that a risk-adapted strategy including ATRA for consolidation provides an outcome improvement in newly diagnosed APL. In addition, our results suggest a benefit in terms of relapse rate reduction using cytarabine coupled to anthracyclines and ATRA during consolidation in the high-risk group.

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25

Adrienn, Rivnyák, Láng András, Péley Bernadette, Nagy Gábor, Nagy Ede, Bóna Adrien, and Goth Kirstin. "AZ IDENTITÁSFEJLŐDÉS FELMÉRÉSE SERDÜLŐKORBAN KÉRDŐÍV MAGYAR ADAPTÁCIÓJA (AIDA-HUNGARY) SERDÜLŐK NEM KLINIKAI MINTÁJÁN." Magyar Pszichológiai Szemle 75, no. 2 (November 10, 2020): 247–69. http://dx.doi.org/10.1556/0016.2020.00015.

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Háttér és célkitűzésekAz Identitásfejlődés Felmérése Serdülőkorban kérdőív (AIDA) az egészséges identitásfejlődés, a normatív identitáskrízis és az identitásdijfúzió elkülönítésére alkalmas önkitöltős módszer a serdülő korosztály részére. A diffúz identitásszerveződés a személyiségzavarok egyik átható jellemzője, ezáltal az AIDA hatékony indikátora lehet a serdülőkori személyiségzavarok korai felismerésének. Tanulmányunk célja az AIDA magyar nyelvre és kultúrára történő adaptációja és pszichometriai jellemzőinek vizsgálata volt nem klinikai mintán.MódszerA faktoranalízisekhez felhasznált minta 522 főből állt, az életkori átlag 15,6 (SD = 1,93) év volt. A validitásvizsgálatokat a teljes minta 366 fős almintáján végeztük el. Az érvényesség ellenőrzéséhez a Képességek és Nehézségek Kérdőívet (SDQ) és a Borderline Személyiségvonások skálát (BPFSC-11) használtuk.EredményekA megerősítő faktoranalízisek alapján a magyar nyelvű AIDA faktorstruktúrája megfelel az eredeti mérőeszköz kétskálás, hat alskálás szerkezetének. A kérdőív illeszkedési mutatói a tudományos kritériumoknak megfelelőek. Az AIDA skálái közepes erősségű korrelációt mutattak az Érzelmi tünetekkel, Viselkedési problémákkal és a Hiperaktivitás skáával, míg erős kapcsolatot mutattak a borderline vonásokkal. A regresszióanalízis alapján az AIDA Identitásdiffúzió összpontszám magas magyarázó erővel bír a borderline vonásokra nézve.KövetkeztetésekAz eredmények alapján az AIDA-Hungary egy megbízható és jól mérő kérdőív, amely alkalmasnak bizonyult a normatív és a patológia irányába mutató identitáskrízis elkülönítésére. Az AIDAezáltal lehetővé teszi a serdülők identitásfejlődésének személyiségfunkciók szempontjából történő felmérését a 12–18 éves korosztálynál, illetve a személyiségfejlődési problémák korai felismerését.Background and aimsAssessment of Identity Development in Adolescence (AIDA) is a self-report questionnaire in order to differentiate healthy identity development from normative identity crisis and from identity diffusion. Diffuse identity is a pervasive feature of personality disorders, thereby AIDA can be a useful indicator in early detection of personality disorders in adolescent. The aim of our study is the Hungarian cultural adaptation of AIDA and to examine its psychometric properties.MethodThe sample used for factor analysis consisted of 522 adolescents with an average age of 15.6 (SD=1.93). Validity tests were performed on a 366 sub-sample of the entire sample. The Strengths and Difficulties Questionnaire (SDQ) and the Borderline Personality Features Scale-11 (BPFSC-11) were used for examine construct validity.ResultsBased on the confirmatory factor analysis, the factor structure of the Hungarian AIDA is consistent with the two scales and six subscales structure of the original instrument. The model-fit indices meet the scientific criteria. AIDA scales displayed moderate correlation with Emotional Symptoms, Behavior problems and Hyperactivity scale, while strong correlation was found with borderline traits. Based on the regression analysis, the AIDA Identity Diffusion total score has a high explanatory power on borderline traits.ConclusionConcerning our results we can assume AIDA-Hungary is a reliable and valid questionnaire, which has proven to be able to distinguish between normative and pathological identity crisis. Thereby it provides a new measurement for adolescents aged 12-18 years to assess identity development in terms of personality functioning and to identify problems of personality development.

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26

Niewerth, Ulla, Andreas Frey, Thomas Voss, Chantal Le Bouguénec, Georg Baljer, Sylvia Franke, and M. Alexander Schmidt. "The AIDA Autotransporter System Is Associated with F18 and Stx2e in Escherichia coli Isolates from Pigs Diagnosed with Edema Disease and Postweaning Diarrhea." Clinical Diagnostic Laboratory Immunology 8, no. 1 (January 1, 2001): 143–49. http://dx.doi.org/10.1128/cdli.8.1.143-149.2001.

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ABSTRACT Pathogenic Escherichia coli strains are known to cause edema disease (ED) and postweaning diarrhea (PWD) in piglets. Although the exact mechanisms of pathogenicity that lead to ED-PWD remain to be elucidated, E. coli-borne Shiga-like toxin and adhesion-mediating virulence factors such as F18 adhesin or F4 fimbriae are believed to play a central role in ED-PWD. In light of these observations we investigated whether another E. coliadhesin, the plasmid-encoded AIDA (adhesin involved in diffuse adherence) might also be present in ED-PWD-causing E. coli isolates. For rapid screening for the AIDA system in large numbers of isolates, a multiplex PCR method along with a duplex Western blot procedure was developed. When screening 104 strains obtained from pigs with or without ED-PWD, we observed a high prevalence of the AIDA operon in porcine E. coli isolates, with over 25% of all strains being AIDA positive, and we could demonstrate a significant association of the intact AIDA gene (orfB) with ED-PWD, while defects in orfB were associated with the absence of disease. Although our data hint toward a contribution of AIDA to ED-PWD, further studies will be necessary since the presence of the AIDA genes was also associated with the presence of the Shiga-like toxin and F18 adhesin genes, two reported virulence factors for ED-PWD.

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27

Liggett, Walter. "AIDA, Version 9/82." American Statistician 39, no. 1 (February 1985): 70. http://dx.doi.org/10.2307/2683915.

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28

Thielemans, Johan. "Aida in de Munt." Documenta 20, no. 1 (March 13, 2019): 63–68. http://dx.doi.org/10.21825/doc.v20i1.10209.

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29

Sollet, Peter C. G. M., Ed J. P. M. de Mol, and Jan H. van Bemmel. "AIDA and medical courseware." Computer Methods and Programs in Biomedicine 25, no. 3 (November 1987): 333–38. http://dx.doi.org/10.1016/0169-2607(87)90092-7.

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30

Bracons, Hélia. "Entrevista com Aida Ferreira." Revista Temas Sociais 1, no. 1 (July 28, 2021): 224–35. http://dx.doi.org/10.53809/2021-01-ts-n.1-224-235.

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31

Lo-Coco, Francesco, Giuseppe Avvisati, Marco Vignetti, Massimo Breccia, Eugenio Gallo, Alessandro Rambaldi, Francesca Paoloni, et al. "Front-line treatment of acute promyelocytic leukemia with AIDA induction followed by risk-adapted consolidation for adults younger than 61 years: results of the AIDA-2000 trial of the GIMEMA Group." Blood 116, no. 17 (October 28, 2010): 3171–79. http://dx.doi.org/10.1182/blood-2010-03-276196.

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AbstractAfter the identification of discrete relapse-risk categories in patients with acute promyelocytic leukemia (APL) receiving all-trans retinoic and idarubicin (AIDA)–like therapies, the Gruppo Italiano Malattie Ematologiche dell'Adulto (GIMEMA) designed a protocol for newly diagnosed APL (AIDA-2000) in which postremission treatment was risk-adapted. Patients with low/intermediate risk received remission at 3 anthracycline-based consolidation courses, whereas high-risk patients received the same schedule as in the previous, non–risk-adapted AIDA-0493 trial including cytarabine. In addition, all patients in the AIDA-2000 received all-trans retinoic acid (ATRA) for 15 days during each consolidation. After induction, 600 of 636 (94.3%) and 420 of 445 (94.4%) patients achieved complete remission in the AIDA-0493 and AIDA-2000, respectively. The 6-year overall survival and cumulative incidence of relapse (CIR) rates were 78.1% versus 87.4% (P = .001) and 27.7% versus 10.7% (P < .0001). Significantly lower CIR rates for patients in the AIDA-2000 were most evident in the high-risk group (49.7% vs 9.3%, respectively, P < .0001). Our data confirm that anthracycline-based consolidation is at least equally effective as cytarabine-containing regimens for low-/intermediate-risk patients and suggest that a risk-adapted strategy including ATRA for consolidation improves outcome in newly diagnosed APL. Furthermore, our results highlight the role of cytarabine coupled to anthracyclines and ATRA during consolidation in the high-risk group. This trial was registered at www.clinicaltrials.gov as #NCT 001064570.

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Testi, Anna Maria, Robin Foa, Gabriella Tomei, Francesco Lo Coco, Andrea Biondi, Andrea Pession, Daniela Diverio, et al. "GIMEMA-AIEOP AIDA Protocols for the Treatment of Newly Diagnosed Acute Promyelocytic Leukemia (APL) In Children: Analysis of 247 Patients Enrolled In Two Sequential Italian Multicenter Trials." Blood 116, no. 21 (November 19, 2010): 871. http://dx.doi.org/10.1182/blood.v116.21.871.871.

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Abstract Abstract 871 Since 1993, Italian pediatric patients (age <18 years) with newly diagnosed acute promyelocytic leukemia (APL) have been enrolled in two consecutive multicenter Gruppo Italiano per le Malattie Ematologiche dell'Adulto (GIMEMA) - Italian Pediatric Hematology and Oncology Group (AIEOP) AIDA 0493 and 2000 trials. The AIDA 0493 protocol consisted of an induction including ATRA (25 mg/m2/day) and idarubicin, followed by three polychemotherapy consolidation courses without ATRA and a four-arm randomized maintenance therapy for patients who were PCR- after consolidation. The AIDA 2000 trial, which started in September 2000, included the same induction followed by a risk-adapted (Sanz criteria, Blood 2004) consolidation. Low and intermediate risk children received three less intensive anthracycline-based courses plus ATRA; for high risk patients, consolidation was intensified by adding ATRA to the three polychemotherapy consolidation courses of the previous protocol. Maintenance therapy consisted of standard daily mercaptopurine and weekly methotrexate given for two years. ATRA was administered for fifteen days every three months during all maintenance therapy. Between January 1993 and June 2000, 124 children were enrolled in the AIDA 0493 protocol. The results of this study have been previously reported (Testi et al, Blood 2005). From July 2000 to January 2009, 123 children with newly diagnosed APL were enrolled in the AIDA 2000 risk-adapted trial. We have now performed an updated analysis of the results of the first study and compared these results with those achieved with the AIDA 2000 study. The median follow-up is 12 and 5 years for the AIDA 0493 and 2000 studies, respectively. No differences in the main clinical and biologic diagnostic patients' characteristics - M/F ratio, median age, median WBC count, FAB M3/M3v, BCR1/BCR2/BCR3, low-intermediate/high risk - were observed between the two groups; the median platelet count was higher in the AIDA 2000 group (27.5 vs 20 × 109/L, p 0.05). The complete remission rate was 96% and 100% in the AIDA 0493 and 2000 protocols, respectively, with no patient showing resistant disease. No toxic death was recorded during the consolidation phase in both protocols; at recovery from the third consolidation course, 97% and 99% of the two groups of patients, tested by RT-PCR, achieved molecular negativity. The 6-year Kaplan-Meier estimates of overall survival (OS) and disease-free survival (DFS) are 89.7% (CI 95%: 84.7–95) vs 96% (CI 95%: 91.7–100), (p 0.05) and 73.1% (CI 95%: 66.7–80.2) vs 82.5% (CI 95%: 75.9–89.8), (p 0.28) in the AIDA 0493 and 2000 protocols, respectively. For low/intermediate risk children, OS and DFS at 6 years are 94.2% (CI 95%: 89.1–99.5) and 76.7% (CI 95%: 68.9–85.4) in the AIDA 0493 vs 95.6% (CI 95%: 90.0–100) and 82.7% (CI 95% 74.9–91.3) in the AIDA 2000 trial, respectively (p 0.57 and 0.73); considering high risk patients, OS and DFS at 6 years are 81.6% (CI 95%: 72.1–92.3) and 65.2% (CI 95%: 54.7–77.6) in the AIDA 0493 vs 96.8% (CI 95%: 90.9–100) and 82.3% (CI 95%: 70.1–96.5) in the AIDA 2000 trial (p 0.05 and 0.20). These results confirm the high anti-leukemic efficacy of the ATRA + idarubicin induction combination. For low/intermediate risk children, the anthracycline-based plus ATRA consolidation is equally effective as the previous cytarabine-containing regimen. The risk-adapted strategy including ATRA for consolidation resulted into a significant improvement in OS for all children. Furthermore, our results highlight the role of cytarabine coupled to anthracyclines and ATRA during consolidation in the high-risk group. Disclosures: Foa: Roche: Consultancy, Speakers Bureau.

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Hananto, Brian Alvin. "Penggunaan Model AIDA Sebagai Struktur Komunikasi Pada Media Sosial Instagram." Jurnal Nawala Visual 1, no. 2 (October 29, 2019): 72–82. http://dx.doi.org/10.35886/nawalavisual.v1i2.24.

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AIDA is a model that explains the steps or levels of consumer perception. Seeing how AIDA is often used as a basis for creating marketing strategies, the author would like to see whether AIDA could be used as a structure for visual communication strategy. The research was done by observing the design process of students from Universitas Pelita Harapan’s Visual Communication Design department. The author had asked the students to design their Instagram’s communication media using the AIDA model as their basis. By using narratology as their design approach, the designers divide timelines and the needs of communication to acts, plots and also Instagram panels. From the design that was done, the author concludes that the usage of structure to create a visual communication strategy helps to define what message is to be made and when should the message be posted on Instagram. The author hoped that this paper had shown the possibility of using AIDA as a communication structure, especially for social media.

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Ngeleka, Musangu, Jane Pritchard, Greg Appleyard, Dorothy M. Middleton, and John M. Fairbrother. "Isolation and Association of Escherichia Coli AIDA-I/STb, Rather than EAST1 Pathotype, with Diarrhea in Piglets and Antibiotic Sensitivity of Isolates." Journal of Veterinary Diagnostic Investigation 15, no. 3 (May 2003): 242–52. http://dx.doi.org/10.1177/104063870301500305.

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To identify emerging Escherichia coli that have the potential to cause diarrhea in pigs, the prevalence of E. coli pathotypes was determined among 170 and 120 isolates from diarrheic and nondiarrheic piglets, respectively. The isolates were tested for F4, F5, F6, F18, and F41 fimbriae, for E. coli attaching and effacing (EAE), porcine attaching and effacing–associated (Paa), and adhesin involved in diffuse adherence (AIDA-I) factors, for LT, STa, STb, and enteroaggregative heat-stable (EAST1) enterotoxins, and for Shiga toxins (Stx1, Stx2, and Stx2e), using DNA hybridization and polymerase chain reaction. All isolates were O-serotyped and tested for antibiotic resistance against 10 drugs. Seventeen different pathotypes, accounting for 40.0% of the isolates, were recovered from diarrheic piglets. The main pathotypes included EAST1 (13.5%), F4/LT/STb/EAST1 (6.5%), AIDA-I/STb/EAST1 (4.1%), F5/STa (2.9%), EAE/EAST1 (2.9%), and AIDA-I/F18 (2.3%). Only 3 pathotypes, EAE (11.7%), EAST1 (10.8%), and EAE/EAST1 (3.3%), were recovered from nondiarrheic piglets. Paa factor was detected in 8.8% and 7.5% of isolates from diarrheic and nondiarrheic piglets, respectively, and always was associated with other virulence determinants. Overall, 22.9% of isolates from diarrheic piglets appeared to be enteropathogens: enterotoxigenic E. coli (11.7%), enteropathogenic E. coli (3.5%), and E. coli isolates (3.0%) for which none of the above adherence factors was detected. Pathotypes AIDA-I/STb/EAST1 and AIDA-I/STb were isolated only from diarrheic piglets and accounted for 4.7% of isolates. Strains of these pathotypes induced diarrhea when inoculated into newborn colostrum-deprived pigs, in contrast to an isolate positive only for EAST1, which did not induce diarrhea. Antibiotic sensitivity test showed that isolates of the AIDA-I/STb/EAST1 and AIDA-I/STb pathotypes were the only strains sensitive to enrofloxacin, gentamicin, neomycin, and trimethoprim–sulfamethoxazole. This study showed that at least 20.5% of isolates from diarrheic piglets appeared to be associated with AIDA-I/STb pathotype and that EAST1 pathotype is probably not an important marker for diarrhea in piglets.

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Wiśniewski, Michał. "Application of Matrix Equations in the Aida Method." Foundations of Management 11, no. 1 (January 1, 2019): 267–76. http://dx.doi.org/10.2478/fman-2019-0022.

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Abstract The article presents the problem of a decision-making process based on the method known as the analysis of interconnected decision areas (AIDA). Author described the basic assumptions of the AIDA method and the classic method of its implementation with the usage of a decision tree. Crucially, the new and innovative improvement in development of the AIDA method is connected with the replacement of the decision tree by the matrix equation to speed up the cost assessment of decision variants.

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Caforio, Alida Linda Patrizia, Giacomo De Luca, Anna Baritussio, Mara Seguso, Nicoletta Gallo, Elisa Bison, Maria Grazia Cattini, et al. "Serum Organ-Specific Anti-Heart and Anti-Intercalated Disk Autoantibodies as New Autoimmune Markers of Cardiac Involvement in Systemic Sclerosis: Frequency, Clinical and Prognostic Correlates." Diagnostics 11, no. 11 (November 22, 2021): 2165. http://dx.doi.org/10.3390/diagnostics11112165.

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Background: Heart involvement (HInv) in systemic sclerosis (SSc) may relate to myocarditis and is associated with poor prognosis. Serum anti-heart (AHA) and anti-intercalated disk autoantibodies (AIDA) are organ and disease-specific markers of isolated autoimmune myocarditis. We assessed frequencies, clinical correlates, and prognostic impacts of AHA and AIDA in SSc. Methods: The study included consecutive SSc patients (n = 116, aged 53 ± 13 years, 83.6% females, median disease duration 7 years) with clinically suspected heart involvement (symptoms, abnormal ECG, abnormal troponin I or natriuretic peptides, and abnormal echocardiography). All SSc patients underwent CMR. Serum AHA and AIDA were measured by indirect immunofluorescence in SSc and in control groups of non-inflammatory cardiac disease (NICD) (n = 160), ischemic heart failure (IHF) (n = 141), and normal blood donors (NBD) (n = 270). AHA and AIDA status in SSc was correlated with baseline clinical, diagnostic features, and outcome. Results: The frequency of AHA was higher in SSc (57/116, 49%, p < 0.00001) than in NICD (2/160, 1%), IHF (2/141, 1%), or NBD (7/270, 2.5%). The frequency of AIDA was higher (65/116, 56%, p < 0.00001) in SSc than in NICD (6/160, 3.75%), IHF (3/141, 2%), or NBD (1/270, 0.37%). AHAs were associated with interstitial lung disease (p = 0.04), history of chest pain (p = 0.026), abnormal troponin (p = 0.006), AIDA (p = 0.000), and current immunosuppression (p = 0.01). AHAs were associated with death (p = 0.02) and overall cardiac events during follow-up (p = 0.017). Conclusions: The high frequencies of AHA and AIDA suggest a high burden of underdiagnosed autoimmune HInv in SSc. In keeping with the negative prognostic impact of HInv in SSc, AHAs were associated with dismal prognosis.

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Maurer, Jochen, Joachim Jose, and Thomas F. Meyer. "Characterization of the Essential Transport Function of the AIDA-I Autotransporter and Evidence Supporting Structural Predictions." Journal of Bacteriology 181, no. 22 (November 15, 1999): 7014–20. http://dx.doi.org/10.1128/jb.181.22.7014-7020.1999.

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ABSTRACT The current model for autodisplay suggests a mechanism that allows a passenger protein to be translocated across the outer membrane by coordinate action of a C-terminal β-barrel and its preceding linking region. The passenger protein, linker, and β-barrel are together termed the autotransporter, while the linker and β-barrel are here referred to as the translocation unit (TU). We characterized the minimal TU necessary for autodisplay with the adhesin-involved-in-diffuse-adherence (AIDA-I) autotransporter. The assumed β-barrel structure at the C terminus of the AIDA-I autotransporter was studied by constructing a set of seven AIDA-I–cholera toxin B subunit fusion proteins containing various portions of AIDA-I. Surface exposure of the cholera toxin B moiety was assessed by dot blot experiments and trypsin accessibility of the chimeric proteins expressed in Escherichia coli JK321 or UT5600. Export of cholera toxin B strictly depended on a complete predicted β-barrel region. The absolute necessity for export of a linking region and its influence on expression as an integral part of the TU was also demonstrated. The different electrophoretic mobilities of native and denatured chimeras indicated that the proposed β-barrel resides within the C-terminal 312 amino acids of AIDA-I. Together these data provide evidence for the predicted β-barrel structure and support our formerly proposed model of membrane topology of the AIDA-I autotransporter.

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WEAS, ABRAM, and MATTHEW CAMPBELL. "Rediscovering the analysis of interconnected decision areas." Artificial Intelligence for Engineering Design, Analysis and Manufacturing 18, no. 3 (August 2004): 227–43. http://dx.doi.org/10.1017/s0890060404040168.

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The method known as the analysis of interconnected decision areas (AIDA) has been in use for nearly 40 years, but has made little headway into engineering design. This paper describes an implementation of AIDA that is useful to engineering designers wishing to combine the solution principles of various subfunctions within a product in new ways. Traditionally, the method is used to understand how one decision affects the options available to other decisions in a large-scale project. The method is to be used interactively with designers participating in a brainstorming session so that ideas are added to AIDA and immediately combined with other compatible ideas. The existing implementation has been tested in a classroom setting in which upper level undergraduates have successfully used the AIDA method along with numerous of design methods to solve conceptual design problems.

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Benz, Inga, Tessa van Alen, Julia Bolte, Mirka E. Wörmann, and M. Alexander Schmidt. "Modulation of transcription and characterization of the promoter organization of the autotransporter adhesin heptosyltransferase and the autotransporter adhesin AIDA-I." Microbiology 156, no. 4 (April 1, 2010): 1155–66. http://dx.doi.org/10.1099/mic.0.032292-0.

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In Gram-negative bacteria, autotransporter proteins constitute the largest family of secreted proteins, and exhibit many different functions. In recent years, research has largely focused on mechanisms of autotransporter protein translocation, where several alternative models are still being discussed. In contrast, the biogenesis of only a few autotransporters has been studied and, likewise, regulation of expression has received only very limited attention. The glycosylated autotransporter adhesin involved in diffuse adherence (AIDA)-I system consists of the aah gene, encoding a specific autotransporter adhesin heptosyltransferase (AAH), and the aidA gene, encoding the autotransporter protein (AIDA-I). In this study, we investigated the promoter organization and transcription of these two genes using reporter plasmids carrying lacZ transcriptional fusions. The two genes, aah and aidA, are transcribed as a bicistronic message. However, aidA is additionally transcribed from its own promoter. There are two distinct start sites for each of the two genes. Interestingly, transcription of both genes is enhanced in hns and rfaH mutant backgrounds. Furthermore, we addressed the influence of environmental factors and different genetic backgrounds of Escherichia coli K-12 strains on transcription activity. We found that transcription varied considerably in different E. coli K-12 laboratory strains and under different growth conditions.

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Robinson, Paul. "Is Aida an orientalist opera?" Cambridge Opera Journal 5, no. 2 (July 1993): 133–40. http://dx.doi.org/10.1017/s0954586700003955.

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Among the more remarkable events of recent intellectual history is that Edward Said, famous avant-garde literary critic, passionate advocate for the Palestinian cause, has begun to write about music. Moreover, not just about any kind of music, but about classical music in the élite (and canonical) European tradition – the symphonies of Beethoven, the operas of Wagner, the chamber music of Schubert and Brahms. Several years ago Said took over the music column in The Nation magazine, and more recently he has published a book, Musical Elaborations, based on a series of invited lectures at the University of California at Irvine.

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Gentil, S., D. Rey, and J. P. Acquadro. "AIDA: A Practical Training Laboratory." IFAC Proceedings Volumes 27, no. 9 (August 1994): 233–36. http://dx.doi.org/10.1016/s1474-6670(17)45938-6.

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Pashootanizadeh, Mitra, and Saideh Khalilian. "Application of the AIDA model." Information and Learning Science 119, no. 11 (November 12, 2018): 635–51. http://dx.doi.org/10.1108/ils-04-2018-0028.

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Purpose The purpose of this study is to measure how effective television programs are in persuading teenagers to use public libraries. Design/methodology/approach This study is a descriptive survey. The statistical population includes all members of public libraries in the City of Isfahan aged between 12 and 16 years (N = 920). Using Cochran’s formula, the sample size was determined to be 270 individuals. The data were collected by a researcher-made questionnaire survey instrument whose validity was confirmed by Library and Information Science experts. Furthermore, the reliability of the questionnaire was confirmed via “Cronbach’s alpha” in the pilot test with 0.73. Having a return rate of 85.93 per cent, the authors were able to analyze 232 sets of responses. Findings Based on the authors’ findings, television programs are only able to satisfy the first stage of the model (i.e. Attention). So, its role in encouraging teenagers to use public libraries is not significant. Among the items of the model, “Desirability and interest in the program” and “Persuading teenagers to use public libraries” were found to be the most and least effective items, respectively, with average responses of 8.42 and 5.13. Moreover, television shows categorized as kids/teenagers were most likely to attract the target audience to libraries. Originality/value There is no any similar study in this scope, especially in the Middle East, where watching the television remains a mainstream activity for teenagers. It is for the first time that AIDA model is used for measuring the effectiveness of television programs in persuading teenagers to use public libraries in Iran.

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Alvarez, Julia. "Dona Aida, With Your Permission." Callaloo 23, no. 3 (2000): 821–23. http://dx.doi.org/10.1353/cal.2000.0116.

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Alvarez, Julia. "Dona Aida, With Your Permission." Callaloo 24, no. 2 (2001): 596–98. http://dx.doi.org/10.1353/cal.2001.0079.

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Linnhoff-Popien, Claudia, and Florentina Hofbauer. "AIDA Cruises – Kreuzfahrt von morgen." Digitale Welt 2, no. 2 (March 7, 2018): 18–24. http://dx.doi.org/10.1007/s42354-018-0067-5.

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Lehmann, E. D., T. Deutsch, E. R. Carson, and P. H. Sönksen. "AIDA: an interactive diabetes advisor." Computer Methods and Programs in Biomedicine 41, no. 3-4 (January 1994): 183–203. http://dx.doi.org/10.1016/0169-2607(94)90054-x.

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Müller, Daniel, Inga Benz, Damini Tapadar, Christian Buddenborg, Lilo Greune, and M. Alexander Schmidt. "Arrangement of the Translocator of the Autotransporter Adhesin Involved in Diffuse Adherence on the Bacterial Surface." Infection and Immunity 73, no. 7 (July 2005): 3851–59. http://dx.doi.org/10.1128/iai.73.7.3851-3859.2005.

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ABSTRACT Autotransporters of gram-negative bacteria are single-peptide secretion systems that consist of a functional N-terminal α-domain (“passenger”) fused to a C-terminal β-domain (“translocator”). How passenger proteins are translocated through the outer membrane has not been resolved, and at present essentially three different models are discussed. In the widely accepted “hairpin model” the passenger proteins are translocated through a channel formed by the β-barrel of the translocator that is integrated in the outer membrane. This model has been challenged by a recent proposal for a general autotransporter model suggesting that there is a hexameric translocation pore that is generated by the oligomerization of six β-domains. A third model suggests that conserved Omp85 participates in autotransporter integration and passenger protein translocation. To examine these models, in this study we investigated the presence of putative oligomeric structures of the translocator of the autotransporter adhesin involved in diffuse adherence (AIDA) in vivo by cross-linking techniques. Furthermore, the capacity of isolated AIDA fusion proteins to form oligomers was studied in vitro by several complementary analytical techniques, such as analytical gel filtration, electron microscopy, immunogold labeling, and cross-linking of recombinant autotransporter proteins in which different passenger proteins were fused to the AIDA translocator. Our results show that the AIDA translocator is mostly present as a monomer. Only a fraction of the AIDA autotransporter was found to form dimers on the bacterial surface and in solution. Higher-order structures, such as hexamers, were not detected either in vivo or in vitro and can therefore be excluded as functional moieties for the AIDA autotransporter.

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Yin, Xianhua, James R. Chambers, Roger Wheatcroft, Roger P. Johnson, Jing Zhu, Bianfang Liu, and Carlton L. Gyles. "Adherence of Escherichia coli O157:H7 Mutants In Vitro and in Ligated Pig Intestines." Applied and Environmental Microbiology 75, no. 15 (June 12, 2009): 4975–83. http://dx.doi.org/10.1128/aem.00297-09.

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ABSTRACT There are contradictory literature reports on the role of verotoxin (VT) in adherence of enterohemorrhagic Escherichia coli O157:H7 (O157 EHEC) to intestinal epithelium. There are reports that putative virulence genes of O island 7 (OI-7), OI-15, and OI-48 of this pathogen may also affect adherence in vitro. Therefore, mutants of vt2 and segments of OI-7 and genes aidA 15 (gene from OI-15) and aidA 48 (gene from OI-48) were generated and evaluated for adherence in vitro to cultured human HEp-2 and porcine jejunal epithelial (IPEC-J2) cells and in vivo to enterocytes in pig ileal loops. VT2-negative mutants showed significant decreases in adherence to both HEp-2 and IPEC-J2 cells and to enterocytes in pig ileal loops; complementation only partially restored VT2 production but fully restored the adherence to the wild-type level on cultured cells. Deletion of OI-7 and aidA 48 had no effect on adherence, whereas deletion of aidA 15 resulted in a significant decrease in adherence in pig ileal loops but not to the cultured cells. This investigation supports the findings that VT2 plays a role in adherence, shows that results obtained in adherence of E. coli O157:H7 in vivo may differ from those obtained in vitro, and identified AIDA-15 as having a role in adherence of E. coli O157:H7.

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Charbonneau, Marie-Ève, and Michael Mourez. "Functional Organization of the Autotransporter Adhesin Involved in Diffuse Adherence." Journal of Bacteriology 189, no. 24 (October 12, 2007): 9020–29. http://dx.doi.org/10.1128/jb.01238-07.

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ABSTRACT The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is a multifunctional autotransporter protein that mediates bacterial aggregation and biofilm formation, as well as adhesion and invasion of cultured epithelial cells. To elucidate the structure-function relationships of AIDA-I, we performed transposon-based linker scanning mutagenesis and constructed mutants with site-directed deletions. Twenty-nine different mutants with insertions that did not affect protein expression were obtained. Eleven mutants were deficient for one or two but not all of the functions associated with the expression of AIDA-I. Functional characterization of the transposon mutants and of an additional deletion mutant suggested that the N-terminal third of mature AIDA-I is involved in binding of this protein to cultured epithelial cells. The purified product of the putative domain could bind to cultured epithelial cells, confirming the importance of this region in adhesion. We also identified several different mutants in which invasion and adhesion were changed to different extents and two mutants in which autoaggregation and biofilm formation were also affected differently. These results suggest that although conceptually linked, adhesion and invasion, as well as autoaggregation and biofilm formation, are phenomena that may rely on distinct mechanisms when they are mediated by AIDA-I. This study sheds new light on the workings of a protein belonging to an emerging family of strikingly versatile virulence factors.

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Caforio, Alida L. P., Anna Baritussio, Renzo Marcolongo, Chun-Yan Cheng, Elena Pontara, Elisa Bison, Maria Grazia Cattini, et al. "Serum Anti-Heart and Anti-Intercalated Disk Autoantibodies: Novel Autoimmune Markers in Cardiac Sarcoidosis." Journal of Clinical Medicine 10, no. 11 (June 2, 2021): 2476. http://dx.doi.org/10.3390/jcm10112476.

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Background: Sarcoidosis is an immune-mediated disease. Cardiac involvement, a granulomatous form of myocarditis, is under-recognized and prognostically relevant. Anti-heart autoantibodies (AHAs) and anti-intercalated disk autoantibodies (AIDAs) are autoimmune markers in nonsarcoidosis myocarditis forms. Objective: The aim was to assess serum AHAs and AIDAs as autoimmune markers in cardiac sarcoidosis. Methods: This is a cross-sectional study on AHA and AIDA frequency in: 29 patients (aged 46 ± 12, 20 male) with biopsy-proven extracardiac sarcoidosis and biopsy-proven or clinically suspected and confirmed by 18-fluorodeoxyglucose positron emission tomography and/or cardiovascular magnetic resonance (CMR) cardiac involvement; 30 patients (aged 44 ± 11, 12 male) with biopsy-proven extracardiac sarcoidosis without cardiac involvement (no cardiac symptoms, normal 12-lead electrocardiogram, echocardiography and CMR), and control patients with noninflammatory cardiac disease (NICD) (n = 160), ischemic heart failure (IHF) (n = 141) and normal blood donors (NBDs) (n = 270). Sarcoidosis patients were recruited in two recruiting tertiary centers in the USA and Italy. AHAs and AIDAs were detected by indirect immunofluorescence on the human myocardium and skeletal muscle. Results: AHA and AIDA frequencies were higher in sarcoidosis with cardiac involvement (86%; 62%) than in sarcoidosis without cardiac involvement (0%; 0%), NICD (8%; 4%), IHF (7%; 2%) and NBD (9%; 0%) (p = 0.0001; p = 0.0001, respectively). Sensitivity and specificity for cardiac sarcoidosis were 86% and 92% for positive AHAs and 62% and 98% for positive AIDAs, respectively. AIDAs in cardiac sarcoidosis were associated with a higher number of involved organs (p = 0.04). Conclusions: Serum AHAs and AIDAs provide novel noninvasive diagnostic autoimmune markers for cardiac sarcoidosis.

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