Journal articles: 'Albert Ehm'

1

Murray, A. "Das Brief- und Memorialbuch des Albert Behaim." English Historical Review 117, no. 473 (September 1, 2002): 950–51. http://dx.doi.org/10.1093/ehr/117.473.950.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Freitas, Lorena Martoni. "Law and literature: the absurd in law in “The stranger” by Albert Camus." ANAMORPHOSIS - Revista Internacional de Direito e Literatura 1, no. 1 (May 20, 2015): 139. http://dx.doi.org/10.21119/anamps.11.139-156/translation.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Borges da Cruz, Leonardo. "A FORMAÇÃO DISCURSIVA PÓS-COLONIAL EM ALBERTO GUERREIRO RAMOS." Revista da Associação Brasileira de Pesquisador@s Negr@s - ABPN 10, no. 25 (2018): 141–64. http://dx.doi.org/10.31418/2177-2770.2018.v10.n.25.p141-164.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Rollason, D. "Le pelerinage a l'epoque carolingienne Bat-Sheva Albert." English Historical Review 115, no. 464 (November 1, 2000): 1257–58. http://dx.doi.org/10.1093/ehr/115.464.1257.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Griffin, R. "Hitlers Bewegung im Urteil der polnischen National-demokratie Albert Kotowski." English Historical Review 115, no. 463 (September 1, 2000): 1028–29. http://dx.doi.org/10.1093/ehr/115.463.1028.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Pauley, B. F. "Antisemitism: A History, ed. Albert S. Lindemann and Richard S. Levy." English Historical Review CXXVI, no. 521 (July 25, 2011): 904–5. http://dx.doi.org/10.1093/ehr/cer198.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Li, Jie. "Innere kulturelle Werte – äußere Partnerschaftsbeziehungen." arbeitstitel | Forum für Leipziger Promovierende 7, no. 1 (October 1, 2019): 21–24. http://dx.doi.org/10.36258/arbeitstitel.v7i1.3321.

Full text

Abstract:

„Die Welt bietet keine Wahrheiten, sondern Liebesmöglichkeiten. Es herrscht das Absurde, und die Liebe errettet davor.“ (Albert Camus, 1997: 60) Liebe und Ehe, so scheint es, sind in der Gesellschaft ein ewiges und übernationales Thema. Durch den Einfluss verschiedener Kulturen gibt es in China und Deutschland unterschiedliche Einstellungen zu Liebe und Ehe. Für die Chinesen sind Ehe und Familie die angestrebten Lebensformen, und es scheint selbstverständlich zu sein, diesbezüglich Harmonie und Loyalität zu bewahren. Hingegen wird bei den Deutschen der Focus (die Priorität) eher auf die Verliebtheit und Freiheit in der Partnerschaft gelegt. Ausgehend von den obigen Annahmen, hat dieser Aufsatz zwei Zielsetzungen: Zum einen werden die unterschiedlichen Vorstellungen über Liebe, Ehe und Partnerschaft in Deutschland und China kontrastiv analysiert und zum anderen sollen Kulturspezifika und Philosophie darin ausführlich erörtert werden. Daraus können wir folgern: Freiheit und Grenzen, Liebe und Respekt gelten als Voraussetzungen für die Liebesbeziehung auf internationaler Ebene.

APA, Harvard, Vancouver, ISO, and other styles

8

Macciuci, Raquel. "Tradición, vanguardia y poética en Madrigal al billete de tranvía de Rafael Alberti." Revista de literatura 71, no. 142 (December 30, 2009): 565–84. http://dx.doi.org/10.3989/revliteratura.2009.v71.i142.93.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Lazaruk, Lance W., Gavin Kernaghan, S. Ellen Macdonald, and Damase Khasa. "Effects of partial cutting on the ectomycorrhizae of Picea glauca forests in northwestern Alberta." Canadian Journal of Forest Research 35, no. 6 (June 1, 2005): 1442–54. http://dx.doi.org/10.1139/x05-062.

Full text

Abstract:

This study assessed the impact of various harvesting practices (including those designed to emulate natural disturbances) on ectomycorrhizae (ECM) associated with white spruce (Picea glauca (Moench) Voss) in northwestern Alberta, Canada. Treatments included clearcuts, partial cuts (dispersed green-tree retention with 20%, 50%, and 75% residual live trees, and aggregated green-tree retention), unharvested control sites, and a burned stand. The percentage of active white spruce root tips and ECM richness and diversity, as observed in soil cores collected throughout the study site, all decreased with increasing disturbance intensity. Effects were particularly pronounced in clearcuts, machine corridors used for access by harvesting equipment in the dispersed green-tree retention stands, and in burned areas. Reductions in ECM biodiversity could be attributed to the sensitivity of late-stage ectomycorrhizae (e.g., Cortinarius spp., Lactarius spp., and Russula spp.) to soil disturbances and changes in microclimate associated with harvesting or burning. Areas of dispersed and aggregated green-tree retention were not dramatically different than unharvested forest in terms of root tip density and ECM richness, diversity, and composition. Harvesting practices that retain a percentage of residual live trees, either dispersed throughout the cutting unit or in aggregated patches, could be an effective means of maintaining ectomycorrhizal biodiversity at the stand level.

APA, Harvard, Vancouver, ISO, and other styles

10

Evans, R. J. W. "Life in an Austro-Hungarian Military Prison: The Slovak Tolstoyan Dr Albert Skarvan's Story." English Historical Review 118, no. 475 (February 1, 2003): 255–56. http://dx.doi.org/10.1093/ehr/118.475.255.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Drolet, M. "God and the Atlantic: America, Europe, and the Religious Divide, by Thomas Albert Howard." English Historical Review 128, no. 534 (September 5, 2013): 1265–67. http://dx.doi.org/10.1093/ehr/cet228.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Sanderson, M. "Prince Albert and the Development of Education in England and Germany in the Nineteenth Century." English Historical Review 117, no. 473 (September 1, 2002): 1004–5. http://dx.doi.org/10.1093/ehr/117.473.1004.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Link, Jason S., and Howard I. Browman. "Operationalizing and implementing ecosystem-based management." ICES Journal of Marine Science 74, no. 1 (January 1, 2017): 379–81. http://dx.doi.org/10.1093/icesjms/fsw247.

Full text

Abstract:

There is now a large literature on ecosystem-based management (EBM; also known as the ecosystem approach). Our sense is that EBM is moving - albeit slowly - from the “what's, why's and when's” to the “how's” of operationalization and implementation; as such it seemed timely to develop this article theme set (TS). Our objectives were to ascertain the state of the discipline and to advance EBM by offering practical examples of its implementation - or attempts at such - in a variety of incarnations and at various scales, including what has or has not worked, suggestions for best practice, and lessons learned. As exemplified by the articles in this TS, key lessons learned include the need for: constant and clear communication with all parties involved; clear objectives and governance; the distillation of complex ecosystem information into digestible indicators; the establishment of reference levels on which management decisions can be made; and clear protocols to evaluate tradeoffs. Instances of truly multisectoral EBM remain rare, with EBM having advanced farthest within specific ocean-use sectors. Although progress towards implementing operational EBM has been somewhat limited, and although EBM is by its very nature complex and difficult to operationalize, there has been progress nonetheless. We hope that this TS will encourage even further operationalization of EBM.

APA, Harvard, Vancouver, ISO, and other styles

14

Roberts, J. "Shorter note. Railways and the Formation of the Italian State in the Nineteenth Century. Albert Schram." English Historical Review 114, no. 457 (June 1, 1999): 758–59. http://dx.doi.org/10.1093/ehr/114.457.758.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Macy, L. "Anglicized ditties: Thomas Watson, Albert Chatterley ed., Italian Madrigals Englished (1590)." Early Music XXX, no. 4 (November 1, 2002): 631–33. http://dx.doi.org/10.1093/em/xxx.4.631.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Storrs, C. "Alberto Radicati, conte di Passerano e Cocconato: Discorsi morali, istorici e politici." English Historical Review CXXV, no. 513 (March 24, 2010): 450–51. http://dx.doi.org/10.1093/ehr/ceq010.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Hsia, R. Po-Chia. "Conflict and Conversion: Catholicism in Southeast Asia, 1500–1700, by Tara Alberts." English Historical Review 130, no. 544 (May 11, 2015): 733–35. http://dx.doi.org/10.1093/ehr/cev085.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Nicholls, A. "Book review. Furr eine Wehrhafte Demokratie. Albert Grzesinski und di Preussiche Politik in der Weimarer Republik. T Albrecht." English Historical Review 115, no. 462 (June 1, 2000): 667–69. http://dx.doi.org/10.1093/ehr/115.462.667.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Dortmans, Brady, William F. Langford, and Allan R. Willms. "An energy balance model for paleoclimate transitions." Climate of the Past 15, no. 2 (March 21, 2019): 493–520. http://dx.doi.org/10.5194/cp-15-493-2019.

Full text

Abstract:

Abstract. A new energy balance model (EBM) is presented and is used to study paleoclimate transitions. While most previous EBMs only dealt with the globally averaged climate, this new EBM has three variants: Arctic, Antarctic and tropical climates. The EBM incorporates the greenhouse warming effects of both carbon dioxide and water vapour, and also includes ice–albedo feedback and evapotranspiration. The main conclusion to be inferred from this EBM is that the climate system may possess multiple equilibrium states, both warm and frozen, which coexist mathematically. While the actual climate can exist in only one of these states at any given time, the EBM suggests that climate can undergo transitions between the states via mathematical saddle-node bifurcations. This paper proposes that such bifurcations have actually occurred in Paleoclimate transitions. The EBM is applied to the study of the Pliocene paradox, the glaciation of Antarctica and the so-called warm, equable climate problem of both the mid-Cretaceous Period and the Eocene Epoch. In all cases, the EBM is in qualitative agreement with the geological record.

APA, Harvard, Vancouver, ISO, and other styles

20

Hoeprich, E. "Review: The Classical clarinet: Albert R. Rice, The clarinet in the Classical period." Early Music 32, no. 4 (November 1, 2004): 621–22. http://dx.doi.org/10.1093/em/32.4.621.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

García Calvo, Margarita. "Tapices de Escenas mitológicas tejidos en los talleres bruselenses de Jacques van der Borcht (h. 1650-h.1709-1713) y Albert Auwercx (1629-1709): Nueva documentación." Archivo Español de Arte 89, no. 355 (September 30, 2016): 241–54. http://dx.doi.org/10.3989/aearte.2016.16.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Ramasubramaniam, A., M. Itakura, M. Ortiz, and E. A. Carter. "Effect of atomic scale plasticity on hydrogen diffusion in iron: Quantum mechanically informed and on-the-fly kinetic Monte Carlo simulations." Journal of Materials Research 23, no. 10 (October 2008): 2757–73. http://dx.doi.org/10.1557/jmr.2008.0340.

Full text

Abstract:

We present an off-lattice, on-the-fly kinetic Monte Carlo (KMC) model for simulating stress-assisted diffusion and trapping of hydrogen by crystalline defects in iron. Given an embedded atom (EAM) potential as input, energy barriers for diffusion are ascertained on the fly from the local environments of H atoms. To reduce computational cost, on-the-fly calculations are supplemented with precomputed strain-dependent energy barriers in defect-free parts of the crystal. These precomputed barriers, obtained with high-accuracy density functional theory calculations, are used to ascertain the veracity of the EAM barriers and correct them when necessary. Examples of bulk diffusion in crystals containing a screw dipole and vacancies are presented. Effective diffusivities obtained from KMC simulations are found to be in good agreement with theory. Our model provides an avenue for simulating the interaction of hydrogen with cracks, dislocations, grain boundaries, and other lattice defects, over extended time scales, albeit at atomistic length scales.

APA, Harvard, Vancouver, ISO, and other styles

23

Merlis, Timothy M., and Matthew Henry. "Simple Estimates of Polar Amplification in Moist Diffusive Energy Balance Models." Journal of Climate 31, no. 15 (August 2018): 5811–24. http://dx.doi.org/10.1175/jcli-d-17-0578.1.

Full text

Abstract:

Diffusive energy balance models (EBMs) that use moist static energy, rather than temperature, as the thermodynamic variable to determine the energy transport provide an idealized framework to understand the pattern of radiatively forced surface warming. These models have a polar amplified warming pattern that is quantitatively similar to general circulation model simulations. Even without surface albedo changes or other spatially varying feedbacks, they simulate polar amplification that results from increased poleward energy transport with warming. Here, two estimates for polar amplification are presented that do not require numerical solution of the EBM governing equation. They are evaluated relative to the results of numerical moist EBM solutions. One estimate considers only changes in a moist thermodynamic quantity (assuming that the increase in energy transport results in a spatially uniform change in moist static energy in the warmed climate) and has more polar amplification than the EBM solution. The other estimate uses a new solution of a truncated form of the moist EBM equation, which allows for a temperature change that is consistent with both the dry and latent energy transport changes, as well as radiative changes. The truncated EBM solution provides an estimate for polar amplification that is nearly identical to that of the numerical EBM solution and only depends on the EBM parameters and climatology of temperature. This solution sheds light on the dependence of polar amplification on the climatological temperature distribution and offers an estimate of the residual polar warming in solar radiation management geoengineered climates.

APA, Harvard, Vancouver, ISO, and other styles

24

Tolkunova, Elena N., Miki Fujioka, Masatomo Kobayashi, Deepali Deka, and James B. Jaynes. "Two Distinct Types of Repression Domain in Engrailed: One Interacts with the Groucho Corepressor and Is Preferentially Active on Integrated Target Genes." Molecular and Cellular Biology 18, no. 5 (May 1, 1998): 2804–14. http://dx.doi.org/10.1128/mcb.18.5.2804.

Full text

Abstract:

ABSTRACT Active transcriptional repression has been characterized as a function of many regulatory factors. It facilitates combinatorial regulation of gene expression by allowing repressors to be dominant over activators under certain conditions. Here, we show that the Engrailed protein uses two distinct mechanisms to repress transcription. One activity is predominant under normal transient transfection assay conditions in cultured cells. A second activity is predominant in an in vivo active repression assay. The domain mediating the in vivo activity (eh1) is highly conserved throughout several classes of homeoproteins and interacts specifically with the Groucho corepressor. While eh1 shows only weak activity in transient transfections, much stronger activity is seen in culture when an integrated target gene is used. In this assay, the relative activities of different repression domains closely parallel those seen in vivo, with eh1 showing the predominant activity. Reducing the amounts of repressor and target gene in a transient transfection assay also increases the sensitivity of the assay to the Groucho interaction domain, albeit to a lesser extent. This suggests that it utilizes rate-limiting components that are relatively low in abundance. Since Groucho itself is abundant in these cells, the results suggest that a limiting component is recruited effectively by the repressor-corepressor complex only on integrated target genes.

APA, Harvard, Vancouver, ISO, and other styles

25

McMaster, Mark E., L. Mark Hewitt, Gerald R. Tetreault, Tamara Janoscik, Chad Boyko, Lisa Peters, Joanne L. Parrott, et al. "Detailed Endocrine Assessments of Wild Fish in the Northern River Basins, Alberta, in Comparison to EEM Monitored Endpoints." Water Quality Research Journal 40, no. 3 (August 1, 2005): 299–314. http://dx.doi.org/10.2166/wqrj.2005.035.

Full text

Abstract:

Abstract During the Northern River Basins Study (NRBS), several issues were highlighted regarding the health of resident fish populations. Based on a basin-wide study of gonad morphology and circulating sex steroid levels, there was preliminary evidence of potential endocrine disruption in fish within the basins. The main concern with this was the potential loss of reproductive capability and possible declining populations. The Northern Rivers Ecosystem Initiative was established in response to the NRBS and an endocrine study was conducted to evaluate in more detail reproductive endocrine function of fish within the identified areas. A suite of endocrine endpoints was used and compared to overall fish health assessments using the Environmental Effects Monitoring (EEM) Programs protocol in areas exposed to sewage and pulp and paper mill effluents. As these northern rivers are nutrient-limited, fish responded to effluent addition (increased nutrients) by showing signs of increased growth and development relative to the upstream reference fish. Some signs of altered reproductive function were observed downstream of the one mill site, however additional responses were observed downstream of a municipal sewage treatment plant discharge from the neighbouring community. The reproductive responses were minimal compared to earlier studies at other older pulp and paper facilities. Follow-up studies at the Grande Prairie site documented changes in the fish responses to the effluents in terms of enrichment. The sewage treatment plant had installed tertiary treatment prior to the latest studies and reductions in the release of nutrients may be the cause. It is not known at the present time whether the pronounced eutrophication response that is still present in these fish may mask other endocrine alterations, as these effluents have endocrine-active compounds in them. The results also emphasize the need for EEM-like monitoring downstream of municipal sewage discharges.

APA, Harvard, Vancouver, ISO, and other styles

26

Bugg, Darrian, Ross Bretherton, Peter Kim, Emily Olszewski, Abigail Nagle, Austin E. Schumacher, Nick Chu, et al. "Infarct Collagen Topography Regulates Fibroblast Fate via p38-Yes-Associated Protein Transcriptional Enhanced Associate Domain Signals." Circulation Research 127, no. 10 (October 23, 2020): 1306–22. http://dx.doi.org/10.1161/circresaha.119.316162.

Full text

Abstract:

Rationale: Myocardial infarction causes spatial variation in collagen organization and phenotypic diversity in fibroblasts, which regulate the heart’s ECM (extracellular matrix). The relationship between collagen structure and fibroblast phenotype is poorly understood but could provide insights regarding the mechanistic basis for myofibroblast heterogeneity in the injured heart. Objective: To investigate the role of collagen organization in cardiac fibroblast fate determination. Methods and Results: Biomimetic topographies were nanofabricated to recapitulate differential collagen organization in the infarcted mouse heart. Here, adult cardiac fibroblasts were freshly isolated and cultured on ECM topographical mimetics for 72 hours. Aligned mimetics caused cardiac fibroblasts to elongate while randomly organized topographies induced circular morphology similar to the disparate myofibroblast morphologies measured in vivo. Alignment cues also induced myofibroblast differentiation, as >60% of fibroblasts formed αSMA (α-smooth muscle actin) stress fibers and expressed myofibroblast-specific ECM genes like Postn (periostin). By contrast, random organization caused 38% of cardiac fibroblasts to express αSMA albeit with downregulated myofibroblast-specific ECM genes. Coupling topographical cues with the profibrotic agonist, TGFβ (transforming growth factor beta), additively upregulated myofibroblast-specific ECM genes independent of topography, but only fibroblasts on flat and randomly oriented mimetics had increased percentages of fibroblasts with αSMA stress fibers. Increased tension sensation at focal adhesions induced myofibroblast differentiation on aligned mimetics. These signals were transduced by p38-YAP (yes-associated protein)-TEAD (transcriptional enhanced associate domain) interactions, in which both p38 and YAP-TEAD (yes-associated protein transcriptional enhanced associate domain) binding were required for myofibroblast differentiation. By contrast, randomly oriented mimetics did not change focal adhesion tension sensation or enrich for p38-YAP-TEAD interactions, which explains the topography-dependent diversity in fibroblast phenotypes observed here. Conclusions: Spatial variations in collagen organization regulate cardiac fibroblast phenotype through mechanical activation of p38-YAP-TEAD signaling, which likely contribute to myofibroblast heterogeneity in the infarcted myocardium.

APA, Harvard, Vancouver, ISO, and other styles

27

Bosworth, R. J. B. "Cattolici e fascisti: La Santa Sede e la politica italiana all'alba del regime (1919-1925), by Alberto Guasco." English Historical Review 129, no. 541 (December 1, 2014): 1537–38. http://dx.doi.org/10.1093/ehr/ceu337.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Zaliapin, I., and M. Ghil. "Another look at climate sensitivity." Nonlinear Processes in Geophysics 17, no. 2 (March 17, 2010): 113–22. http://dx.doi.org/10.5194/npg-17-113-2010.

Full text

Abstract:

Abstract. We revisit a recent claim that the Earth's climate system is characterized by sensitive dependence to parameters; in particular, that the system exhibits an asymmetric, large-amplitude response to normally distributed feedback forcing. Such a response would imply irreducible uncertainty in climate change predictions and thus have notable implications for climate science and climate-related policy making. We show that equilibrium climate sensitivity in all generality does not support such an intrinsic indeterminacy; the latter appears only in essentially linear systems. The main flaw in the analysis that led to this claim is inappropriate linearization of an intrinsically nonlinear model; there is no room for physical interpretations or policy conclusions based on this mathematical error. Sensitive dependence nonetheless does exist in the climate system, as well as in climate models – albeit in a very different sense from the one claimed in the linear work under scrutiny – and we illustrate it using a classical energy balance model (EBM) with nonlinear feedbacks. EBMs exhibit two saddle-node bifurcations, more recently called "tipping points," which give rise to three distinct steady-state climates, two of which are stable. Such bistable behavior is, furthermore, supported by results from more realistic, nonequilibrium climate models. In a truly nonlinear setting, indeterminacy in the size of the response is observed only in the vicinity of tipping points. We show, in fact, that small disturbances cannot result in a large-amplitude response, unless the system is at or near such a point. We discuss briefly how the distance to the bifurcation may be related to the strength of Earth's ice-albedo feedback.

APA, Harvard, Vancouver, ISO, and other styles

29

Berger, S. "Religion and the Rise of Historicism: W. M. L. de Wette, Jacob Burckhardt, and the Theological Origins of Nineteenth-Century Historical Consciousness Thopmas Albert Howard." English Historical Review 115, no. 464 (November 1, 2000): 1330–31. http://dx.doi.org/10.1093/ehr/115.464.1330.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Grant, Alasdair C. "Enrico Pisano: Liber Maiorichinus de gestis Pisanorum illustribus, ed., with introduction, Giuseppe Scalia, commentary Alberto Bartola, tr. Marco Guardo." English Historical Review 134, no. 569 (June 12, 2019): 957–59. http://dx.doi.org/10.1093/ehr/cez144.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Osborne, Toby. "One Foot in the Palace: The Habsburg Court of Brussels and the Politics of Access in the Reign of Albert and Isabella, 1598–1621, by Dries Raeymaekers." English Historical Review 130, no. 544 (June 2015): 743–44. http://dx.doi.org/10.1093/ehr/cev096.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Kypke, Kolja Leon, William Finlay Langford, and Allan Richard Willms. "Anthropocene climate bifurcation." Nonlinear Processes in Geophysics 27, no. 3 (July 8, 2020): 391–409. http://dx.doi.org/10.5194/npg-27-391-2020.

Full text

Abstract:

Abstract. This article presents the results of a bifurcation analysis of a simple energy balance model (EBM) for the future climate of the Earth. The main focus is on the following question: can the nonlinear processes intrinsic to atmospheric physics, including natural positive feedback mechanisms, cause a mathematical bifurcation of the climate state, as a consequence of continued anthropogenic forcing by rising greenhouse gas emissions? Our analysis shows that such a bifurcation could cause an abrupt change to a drastically different climate state in the EBM, which is warmer and more equable than any climate existing on Earth since the Pliocene epoch. In previous papers, with this EBM adapted to paleoclimate conditions, it was shown to exhibit saddle-node and cusp bifurcations, as well as hysteresis. The EBM was validated by the agreement of its predicted bifurcations with the abrupt climate changes that are known to have occurred in the paleoclimate record, in the Antarctic at the Eocene–Oligocene transition (EOT) and in the Arctic at the Pliocene–Paleocene transition (PPT). In this paper, the EBM is adapted to fit Anthropocene climate conditions, with emphasis on the Arctic and Antarctic climates. The four Representative Concentration Pathways (RCP) considered by the IPCC (Intergovernmental Panel on Climate Change) are used to model future CO2 concentrations, corresponding to different scenarios of anthropogenic activity. In addition, the EBM investigates four naturally occurring nonlinear feedback processes which magnify the warming that would be caused by anthropogenic CO2 emissions alone. These four feedback mechanisms are ice–albedo feedback, water vapour feedback, ocean heat transport feedback, and atmospheric heat transport feedback. The EBM predicts that a bifurcation resulting in a catastrophic climate change, to a pre-Pliocene-like climate state, will occur in coming centuries for an RCP with unabated anthropogenic forcing, amplified by these positive feedbacks. However, the EBM also predicts that appropriate reductions in carbon emissions may limit climate change to a more tolerable continuation of what is observed today. The globally averaged version of this EBM has an equilibrium climate sensitivity (ECS) of 4.34 K, near the high end of the likely range reported by the IPCC.

APA, Harvard, Vancouver, ISO, and other styles

33

Kalus, Oren. "To Make the Familiar Strange— Aesthetic Derealization in the Work of Alberto Giacometti." Empirical Studies of the Arts 28, no. 1 (January 2010): 19–35. http://dx.doi.org/10.2190/em.28.1.c.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Triano Milán, José Manuel. "REIXACH SALA, Albert, Finances publiques i mobilitat social a la Catalunya de la Baixa Edat Mitjana: Girona, 1340-1440." Edad Media. Revista de Historia, no. 21 (July 13, 2020): 546–48. http://dx.doi.org/10.24197/em.21.2020.546-548.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Neubauer, Heidi A., and Stuart M. Pitson. "Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence." F1000Research 5 (December 6, 2016): 2825. http://dx.doi.org/10.12688/f1000research.10336.1.

Full text

Abstract:

Sphingosine kinase 2 (SK2) is a ubiquitously expressed lipid kinase that has important, albeit complex and poorly understood, roles in regulating cell survival and cell death. In addition to being able to promote cell cycle arrest and apoptosis under certain conditions, it has recently been shown that SK2 can promote neoplastic transformation and tumorigenesis in vivo. Therefore, well validated and reliable tools are required to study and better understand the true functions of SK2. Here, we compare two commercially available SK2 antibodies: a rabbit polyclonal antibody from Proteintech that recognizes amino acids 266-618 of human SK2a, and a rabbit polyclonal antibody from ECM Biosciences that recognizes amino acids 36-52 of human SK2a. We examine the performance of these antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence staining of endogenous SK2, using human HEK293 and HeLa cell lines, as well as mouse embryonic fibroblasts (MEFs). Furthermore, we assess the specificity of these antibodies to the target protein through the use of siRNA-mediated SK2 knockdown and SK2 knockout (Sphk2-/-) MEFs. Our results demonstrate that the Proteintech anti-SK2 antibody reproducibly displayed superior sensitivity and selectivity towards SK2 in immunoblot analyses, while the ECM Biosciences anti-SK2 antibody was reproducibly superior for SK2 immunoprecipitation and detection by immunofluorescence staining. Notably, both antibodies produced non-specific bands and staining in the MEFs, which was not observed with the human cell lines. Therefore, we conclude that the Proteintech SK2 antibody is a valuable reagent for use in immunoblot analyses, and the ECM Biosciences SK2 antibody is a useful tool for SK2 immunoprecipitation and immunofluorescence staining, at least in the human cell lines employed in this study.

APA, Harvard, Vancouver, ISO, and other styles

36

Neubauer, Heidi A., and Stuart M. Pitson. "Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence." F1000Research 5 (March 23, 2017): 2825. http://dx.doi.org/10.12688/f1000research.10336.2.

Full text

Abstract:

Sphingosine kinase 2 (SK2) is a ubiquitously expressed lipid kinase that has important, albeit complex and poorly understood, roles in regulating cell survival and cell death. In addition to being able to promote cell cycle arrest and apoptosis under certain conditions, it has recently been shown that SK2 can promote neoplastic transformation and tumorigenesis in vivo. Therefore, well validated and reliable tools are required to study and better understand the true functions of SK2. Here, we compare two commercially available SK2 antibodies: a rabbit polyclonal antibody from Proteintech that recognizes amino acids 266-618 of human SK2a, and a rabbit polyclonal antibody from ECM Biosciences that recognizes amino acids 36-52 of human SK2a. We examine the performance of these antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence staining of endogenous SK2, using human HEK293 and HeLa cell lines, as well as mouse embryonic fibroblasts (MEFs). Furthermore, we assess the specificity of these antibodies to the target protein through the use of siRNA-mediated SK2 knockdown and SK2 knockout (Sphk2-/-) MEFs. Our results demonstrate that the Proteintech anti-SK2 antibody reproducibly displayed superior sensitivity and selectivity towards SK2 in immunoblot analyses, while the ECM Biosciences anti-SK2 antibody was reproducibly superior for SK2 immunoprecipitation and detection by immunofluorescence staining. Notably, both antibodies produced non-specific bands and staining in the MEFs, which was not observed with the human cell lines. Therefore, we conclude that the Proteintech SK2 antibody is a valuable reagent for use in immunoblot analyses, and the ECM Biosciences SK2 antibody is a useful tool for SK2 immunoprecipitation and immunofluorescence staining, at least in the human cell lines employed in this study.

APA, Harvard, Vancouver, ISO, and other styles

37

Henry, Symon. "ECM+Génération2014 : pistes de réflexion sur la notion de style chez les compositeurs de la relève canadienne." Les Cahiers de la Société québécoise de recherche en musique 15, no. 2 (April 13, 2016): 71–85. http://dx.doi.org/10.7202/1036120ar.

Full text

Abstract:

En 2014, l’Ensemble contemporain de Montréal (ECM+), dirigé par Véronique Lacroix, célébrait le 20e anniversaire du projet Génération, concours pancanadien bisannuel de composition. Cet article étudie les démarches des compositeurs qui ont participé à l’édition 2014 : Marie-Pierre Brasset (Québec), Alec Hall (Ontario/New-York), Evelin Ramon (Québec/La Havane) et Anthony Tan (Alberta/Berlin). Y sont proposées certaines bases de réflexion quant à la notion de style appliquée aux démarches de compositeurs de la relève liés à la scène canadienne de musique contemporaine. L’article circonscrit d’abord l’idée de style telle qu’elle a été comprise dans le cadre de cet article. Est ensuite mise en perspective, grâce à cette définition de concept, les démarches des lauréats du projet ECM+ Génération2014. Ceux-ci partagent un certain dialecte stylistique, tout en ayant une forte volonté d’indépendance. On remarque particulièrement un positionnement fort, chez les quatre compositeurs, par rapport aux archétypes de la musique classique occidentale – comme le lyrisme traditionnel et l’expression personnelle. L’idée de recherche et d’expérimentation constante s’est aussi révélée être un angle d’approche important afin d’étudier la notion de style chez les quatre lauréats. L’expression personnelle a été le dernier enjeu majeur commun ayant émergé de cette étude. En conclusion, la prise en charge critique, par les compositeurs, de l’ensemble de contraintes qui définit leur donné stylistique, s’est révélé être un outil de création de sens puissant dans leur travail.

APA, Harvard, Vancouver, ISO, and other styles

38

Navarro Baena, Alberto. "NAVARRO BAENA, Alberto, El cabildo catedral de León: clero, monarquía y sociedad (1073-1289)." Edad Media. Revista de Historia, no. 21 (July 17, 2020): 568–71. http://dx.doi.org/10.24197/em.21.2020.568-571.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Xiong, Wen-cheng, and J. Thomas Parsons. "Induction of Apoptosis after Expression of PYK2, a Tyrosine Kinase Structurally Related to Focal Adhesion Kinase." Journal of Cell Biology 139, no. 2 (October 20, 1997): 529–39. http://dx.doi.org/10.1083/jcb.139.2.529.

Full text

Abstract:

Many cells (e.g., epithelial cells) require attachment to the extracellular matrix (ECM) to survive, a phenomenon known as anchorage-dependent cell survival. Disruption of the cell–ECM interactions mediated by the integrin receptors results in apoptosis. Focal adhesion kinase (FAK), a 125-kD protein tyrosine kinase activated by integrin engagement, appears to be involved in mediating cell attachment and survival. Proline-rich tyrosine kinase 2 (PYK2), also known as cellular adhesion kinase β (CAKβ) and related adhesion focal tyrosine kinase, is a second member of the FAK subfamily and is activated by an increase in intracellular calcium levels, or treatment with TNFα and UV light. However, the function of PYK2 remains largely unknown. In this study, we show that over-expression of PYK2, but not FAK, in rat and mouse fibroblasts leads to apoptotic cell death. Using a series of deletion mutants and chimeric fusion proteins of PYK2/FAK, we determined that the NH2-terminal domain and tyrosine kinase activity of PYK2 were required for the efficient induction of apoptosis. Furthermore, the apoptosis mediated by PYK2 could be suppressed by over-expressing catalytically active v-Src, c-Src, phosphatidylinositol-3-kinase, or Akt/protein kinase B. In addition, it could also be suppressed by overexpressing an ICE or ICE-like proteinase inhibitor, crmA, but not Bcl2. Collectively, our results suggest that PYK2 and FAK, albeit highly homologous in primary structure, appear to have different functions; FAK is required for cell survival, whereas PYK2 induces apoptosis in fibroblasts.

APA, Harvard, Vancouver, ISO, and other styles

40

Alemany, Francisco, Itziar Álvarez, Alberto García, Dolores Cortés, Teodoro Ramírez, José Quintanilla, Federico Álvarez, and José María Rodríguez. "Postflexion larvae and juvenile daily growth patterns of the Alborán Sea sardine (Sardina pilchardus Walb.): influence of wind." Scientia Marina 70, S2 (October 30, 2006): 93–104. http://dx.doi.org/10.3989/scimar.2006.70s293.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Huang, Jin, and Kenneth P. Bowman. "Climate Sensitivity and Equilibrium Climate States Of A Two-Dimensional Energy Balance Model." Annals of Glaciology 14 (1990): 340. http://dx.doi.org/10.1017/s0260305500009034.

Full text

Abstract:

A two-dimensional energy balance model (EBM) with a seasonal cycle and a realistic land–ocean distribution is used to study climate sensitivity and the properties of equilibrium climate states. The land–ocean distribution is represented by the heat capacity of the surface. The horizontal heat flux is parameterized by diffusion. Nonlinearity is introduced into the model by the albedo–temperature feedback. A multi-grid finite difference method is used to solve the model equation. This method shows great advantages compared to other numerical methods. The sensitivity of snow cover to changes in the solar constant is examined. It is found that the climate is more sensitive in summer due to a more effective albedo–temperature feedback. The local annual mean temperature and the amplitude of the seasonal cycle depend on the land–ocean distribution. Because Eurasia has the largest annual cycle of temperature and is the coldest in winter, winter snow appears first in Eurasia as the solar constant is reduced. Wintertime snow appears next in Greenland because of its low annual mean temperature and last in North America. Perennial snow cover appears first in Greenland because of the low annual mean temperature and the small annual cycle, and next appears in North America. Perennial snow in Eurasia appears only when the solar constant is reduced to a very low value, since the Eurasian land mass is too hot in summer for perennial snow cover. The sensitivity of the model climate to changes in the orbital parameters is also investigated and discussed. Small polar ice caps in one-dimensional mean-annual EBMs are unstable to small perturbations. The ice caps either vanish or grow to a stable finite size. This phenomenon, referred to as the small ice-cap instability (SICI), is a consequence of the multiple stable equilibrium states in one-dimensional models. The SICI may be related to glacial–interglacial transitions. However, numerical results with the two-dimensional EBM demonstrate that the existence of the SICI depends on the land–ocean distribution. The SICI exists in the southern hemisphere, but not in the northern hemisphere. This casts doubt on the role of the SICI in northern hemisphere glaciations. Experiments with a one-dimensional seasonal EBM with simplified geography have been made to analyze the two-dimensional results. Mechanisms for the existence of the SICI in seasonal EBMs are discussed.

APA, Harvard, Vancouver, ISO, and other styles

42

Huang, Jin, and Kenneth P. Bowman. "Climate Sensitivity and Equilibrium Climate States Of A Two-Dimensional Energy Balance Model." Annals of Glaciology 14 (1990): 340. http://dx.doi.org/10.3189/s0260305500009034.

Full text

Abstract:

A two-dimensional energy balance model (EBM) with a seasonal cycle and a realistic land–ocean distribution is used to study climate sensitivity and the properties of equilibrium climate states. The land–ocean distribution is represented by the heat capacity of the surface. The horizontal heat flux is parameterized by diffusion. Nonlinearity is introduced into the model by the albedo–temperature feedback. A multi-grid finite difference method is used to solve the model equation. This method shows great advantages compared to other numerical methods.The sensitivity of snow cover to changes in the solar constant is examined. It is found that the climate is more sensitive in summer due to a more effective albedo–temperature feedback. The local annual mean temperature and the amplitude of the seasonal cycle depend on the land–ocean distribution. Because Eurasia has the largest annual cycle of temperature and is the coldest in winter, winter snow appears first in Eurasia as the solar constant is reduced. Wintertime snow appears next in Greenland because of its low annual mean temperature and last in North America. Perennial snow cover appears first in Greenland because of the low annual mean temperature and the small annual cycle, and next appears in North America. Perennial snow in Eurasia appears only when the solar constant is reduced to a very low value, since the Eurasian land mass is too hot in summer for perennial snow cover. The sensitivity of the model climate to changes in the orbital parameters is also investigated and discussed.Small polar ice caps in one-dimensional mean-annual EBMs are unstable to small perturbations. The ice caps either vanish or grow to a stable finite size. This phenomenon, referred to as the small ice-cap instability (SICI), is a consequence of the multiple stable equilibrium states in one-dimensional models. The SICI may be related to glacial–interglacial transitions. However, numerical results with the two-dimensional EBM demonstrate that the existence of the SICI depends on the land–ocean distribution. The SICI exists in the southern hemisphere, but not in the northern hemisphere. This casts doubt on the role of the SICI in northern hemisphere glaciations. Experiments with a one-dimensional seasonal EBM with simplified geography have been made to analyze the two-dimensional results. Mechanisms for the existence of the SICI in seasonal EBMs are discussed.

APA, Harvard, Vancouver, ISO, and other styles

43

Bucheli, Marcelo. "Canadian Multinational Corporations and Economic Nationalism: The Case of Imperial Oil Limited in Alberta (Canada) and Colombia, 1899-1938." Entreprises et histoire 54, no. 1 (2009): 67. http://dx.doi.org/10.3917/eh.054.0067.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Han, Dae Geun, Chi Bum Ahn, Ji-Hyun Lee, Yongsung Hwang, Joo Hyun Kim, Kook Yang Park, Jin Woo Lee, and Kuk Hui Son. "Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation." Polymers 11, no. 4 (April 9, 2019): 643. http://dx.doi.org/10.3390/polym11040643.

Full text

Abstract:

Due to the morphological resemblance between the electrospun nanofibers and extracellular matrix (ECM), electrospun fibers have been widely used to fabricate scaffolds for tissue regeneration. Relationships between scaffold morphologies and cells are cell type dependent. In this study, we sought to determine an optimum electrospun fiber diameter for human vascular smooth muscle cell (VSMC) regeneration in vascular scaffolds. Scaffolds were produced using poly(caprolactone) (PCL) electrospun fiber diameters of 0.5, 0.7, 1, 2, 2.5, 5, 7 or 10 μm, and VSMC survivals, proliferations, infiltrations, and phenotypes were recorded after culturing cells on these scaffolds for one, four, seven, or 10 days. VSMC phenotypes and macrophage infiltrations into scaffolds were evaluated by implanting scaffolds subcutaneously in a mouse for seven, 14, or 28 days. We found that human VSMC survival was not dependent on the electrospun fiber diameter. In summary, increasing fiber diameter reduced VSMC proliferation, increased VSMC infiltration and increased macrophage infiltration and activation. Our results indicate that electrospun PCL fiber diameters of 7 or 10 µm are optimum in terms of VSMC infiltration and macrophage infiltration and activation, albeit at the expense of VSMC proliferation.

APA, Harvard, Vancouver, ISO, and other styles

45

Goh, A. Y., and S. S. M. Hussain. "Sudden hearing loss and pregnancy: a review." Journal of Laryngology & Otology 126, no. 4 (February 6, 2012): 337–39. http://dx.doi.org/10.1017/s0022215112000114.

Full text

Abstract:

AbstractBackground:Acute hearing loss is a distressing albeit rare occurrence in pregnancy. Due to its infrequent presentation, little is known of its cause, and the best management is unclear.Objective:To present a summary of current knowledge regarding sudden hearing loss in pregnancy and its management, by reviewing the published literature.Data sources:The following electronic databases: PubMed (MEDLINE), Ovid (MEDLINE), all EBM Reviews (Cochrane DSR, ACP Journal Club, DARE and CCTR) and Embase; plus a hand search of reference lists of retrieved papers.Study selection and data extraction:All papers retrieved using key word searches for ‘sudden hearing loss and pregnancy’ and ‘sudden deafness and pregnancy’ were reviewed. Due to the scarcity of literature, all studies identified were included in this review.Data synthesis and conclusions:All retrieved papers were reviewed. Sudden hearing loss is a rare occurrence during pregnancy, leading to a dearth of literature due to limited clinical experience. Audiological investigation findings for such patients remain equivocal. Recovery can be spontaneous during the postpartum period. In patients who require medical treatment, a dextran 40 infusion can be employed. There remains scope for further research, in the form of larger studies, to ascertain the best management option for this clinical problem.

APA, Harvard, Vancouver, ISO, and other styles

46

Khouly, Ismael, Rosalie Salus Braun, Michelle Ordway, Iya Ghassib, Lena Larsson, and Farah Asa’ad. "The Role of Epigenetics in Periodontal and Systemic Diseases and Smoking: A Systematic Review." Applied Sciences 11, no. 11 (June 6, 2021): 5269. http://dx.doi.org/10.3390/app11115269.

Full text

Abstract:

The aims of this systematic review were to identify and synthesize the evidence for an association in DNA methylation/histone modifications between periodontal diseases and systemic diseases/smoking. Electronic database searches using relevant search terms in PubMed, Embase, MEDLINE, CINAHL, Web of Science, Scopus, and SciELO, and manual searches, were independently conducted to identify articles meeting the inclusion criteria. Nine studies of 1482 participants were included. Periodontitis was compared to metabolic disorders, rheumatoid arthritis (RA), cancer, and smokers, as well as healthy controls. Substantial variation regarding the reporting of sample sizes and patient characteristics, statistical analyses, and methodology was found. IL6 and TNF were modified similarly in RA and periodontitis. While TIMP-3 and GSTP-1 were significantly lower in periodontitis patients and controls than in cancer, SOCS-1, RMI2, CDH1, and COX2 were modified similarly in both cancer and periodontitis. While TLR4 in and CXCL8 were affected in periodontitis independent of smoking habit, smoking might change the transcription and methylation states of ECM organization-related genes, which exacerbated the periodontal condition. There was some evidence, albeit inconsistent, for an association between DNA methylation and periodontal diseases and systemic diseases or smokers compared to healthy patients or non-smokers.

APA, Harvard, Vancouver, ISO, and other styles

47

DeCarlo, Thomas, Tirthankar Roy, and Michael Barone. "How sales manager experience and historical data trends affect decision making." European Journal of Marketing 49, no. 9/10 (September 14, 2015): 1484–504. http://dx.doi.org/10.1108/ejm-05-2014-0301.

Full text

Abstract:

Purpose – The purpose of this study is to examine how trends in historical data influence two types of predictive judgments: territory selection and salesperson hiring. Sales managers are confronted frequently with decisions that explicitly or implicitly involve forecasting with limited information. In doing so, they conceptualize how the magnitude of these trend effects may be affected by the experience managers have in making these types of judgments. Study 1 provides evidence of a curvilinear relationship between experience and reliance on the trend data whereby the sales territory selections of novice sales managers exhibited greater susceptibility to informational trends than did the evaluations of naïve and expert decision-makers. A benchmark analysis in Study 2 further revealed that the salesperson selections made by novice and expert sales managers were equally biased, albeit in opposite directions, with novices overweighting and experts underweighting historical performance trends. Implications of these findings are discussed, as are avenues for future research. Design/methodology/approach – The authors employ an online experimental design methodology of practicing managers. For Study 1, they use regression, whereas Study 2 uses a deterministic process to develop a priori predictive benchmark forecasts. Ordinary least squares is then used to estimate manager’s decisions, which are then compared to the predictive forecasts to determine accuracy. Findings – Study 1 provides evidence of a curvilinear relationship between experience and reliance on the trend data whereby the sales territory selections of novice sales managers exhibited greater susceptibility to informational trends than did the evaluations of naïve and expert decision-makers. A benchmark analysis in Study 2 further revealed that the salesperson selections made by novice and expert sales managers were equally biased, albeit in opposite directions, with novices overweighting and experts underweighting historical performance trends. Originality/value – The present inquiry is the first to provide insights into an important issue that has been the subject of equivocal findings, namely, whether experience in a judgmental domain exerts a facilitating or debilitating effect on sales manager decision-making. In this regard, some research supports the intuition that experience in making a particular type of decision can insulate managers from judgmental bias and, in doing so, improve decision quality (see Shanteau, [1992a] for a summary). In contrast, other work provides a more pessimistic view by demonstrating that the quality of decision-making is either unaffected by or can erode with additional experience (Hutchinson et al., 2010). To help reconcile these conflicting findings, the authors presented and tested a theoretical framework conceptualizing how trends may influence predictive judgments across three levels of decision-maker experience.

APA, Harvard, Vancouver, ISO, and other styles

48

Matsuura, Shinobu, Cristal Reyna Thompson, Seng Kah Ng, Aikaterini Karagianni, Christina Ward Torres, Carla Silvana Mazzeo, Orly Leiva, Alessandro Malara, Alessandra Balduini, and Katya Ravid. "Integrin-Mediated Adhesion to Extracellular Matrix Protein Fibronectin Drives Megakaryocytosis in JAK2V617F+ Primary Myelofibrosis." Blood 134, Supplement_1 (November 13, 2019): 4205. http://dx.doi.org/10.1182/blood-2019-132124.

Full text

Abstract:

Excessive accumulation of extracellular matrix (ECM) is a hallmark of bone marrow (BM) milieu in Primary Myelofibrosis (PMF). Myelofibrosis was long regarded as a bystander of disease in PMF. However, as cells have the ability to sense the surrounding ECM through integrin receptors, we examined the hypothesis that abnormal ECM in myelofibrosis, mediated by integrin activation, contributes to BM megakaryocytosis in JAK2V617F+ PMF. Due to its complex genetic landscape, modeling PMF in animals is challenging. While there is no ideal model to replicate the human disease, Vav1-hJAK2V617F transgenic mice (JAK2V617F+) (Xing S et al. Blood. 2008; 111:5109-5117) are most appropriate for our studies because they express the most frequent mutation in PMF and show megakaryocytosis in BM and a predictable development of myelofibrosis throughout the animal's life. Fibronectin (FN) is a dimeric ECM glycoprotein produced by a variety of cells in BM. FN secretion by BM stromal cells from PMF patients correlates with fibrosis and disease severity. Analysis of myelofibrotic BM from JAK2V617F+ mice revealed elevated levels of FN in BM ECM. Integrins are heterodimeric cell adhesion receptors of α and β subunits that connect the cell cytoskeleton to ECM. Integrin engagement and subsequent signaling affect cell differentiation, proliferation, migration and survival. Integrins α5β1, α4β1, αIIbβ3, and αυβ3 are FN-binding integrins known to be expressed in megakaryocytes. Expression analysis detected significantly elevated expression of α5, αIIb, αυ and β3 subunits in CD41+ megakaryocytes fromJAK2V617F+ mice in vitro and in vivo. Expression of β1 integrin was unchanged in megakaryocytes fromJAK2V617F+ mice, but expression of 9EG7, an antibody clone that detects the high-affinity conformation of β1 integrin, was elevated in JAK2V617F+ CD41+ megakaryocytes. Levels of the high-affinity form of β1 integrin decrease in response to inhibition of α5 integrin function (using Hmα5-1 antibody), indicating a high degree of correlation between affinity to FN and β1 activation in megakaryocytes fromJAK2V617F+ mice. As suggested by expression studies, in-vitro differentiated JAK2V617F+ megakaryocytes showed increased affinity to FN in adhesion assays. To ascertain the contribution of adhesion to FN in development of megakaryocytosis, megakaryocytes were differentiated in vitro on FN, with and without the presence of α5 integrin inhibitory antibody Hmα5-1. Culture on FN had a positive effect on the number of CD41+ megakaryocytes after four days of culture, especially in JAK2V617F+ BM. Importantly, inhibition of α5 integrin decreased the number of CD41+ megakaryocytes in JAK2V617F+ BM to nearly wild-type levels. To determine the effect of in vivo inhibition of α5 integrin on megakaryocyte numbers, the antibody clone 5H10-27, or MFR-5, previously reported to be effective in vivo, was used. Effective targeting in vivo of BM megakaryocytes by the 5H10-27 antibody was confirmed using a fluorochrome-labelled secondary antibody specific to the isotype of the injected clone (Rat IgG2a, κ). The same method was used to determine minimum required dose and interval of administration. JAK2V617F+ mice were treated with three doses of 5H10-27 or isotype control antibody (1mg/Kg IV every 48 hours). Analysis of treated animals on day 5 detected a decrease in megakaryocyte α5 integrin expression in animals treated with 5H10-27 antibody relative to control isotype antibody-treated animals. Platelet counts in peripheral blood and percentage of BM CD41+ megakaryocytes were consistently, albeit non-significantly, lower in 5H10-27 antibody-treated than in isotype antibody-treated animals. Corroborating our findings in mice, analysis of megakaryocytes from patients carrying the JAK2V617F mutation revealed elevated cell surface expression of α5 integrin subunit and increased adhesion to FN, which was dampened by an anti-α5 integrin antibody (Sam-1). Our results uncovered a global de-regulation of FN-binding integrin expression in megakaryocytes carrying the JAK2V617F mutation and the central role of FN-α5 integrin in development of megakaryocytosis in PMF. These results challenge the current paradigm by bringing ECM and myelofibrosis from a bystander position to center stage in the pathology of PMF. Disclosures No relevant conflicts of interest to declare.

APA, Harvard, Vancouver, ISO, and other styles

49

Mavraganis, V. G., N. T. Papadopoulos, and N. A. Kouloussis. "Extract of olive fruit fly males (Diptera: Tephritidae) attract virgin females." ENTOMOLOGIA HELLENICA 19, no. 1 (June 1, 2017): 14. http://dx.doi.org/10.12681/eh.11590.

Full text

Abstract:

Research conducted during the past three decades suggests that in contrast to most other tephritid fruit flies, in which sexual pheromones are produced by males, the olive fruit fly Bactrocera oleae (Rossi) relies for its sexual communication on a pheromone that is produced by females. However, our present study suggests that virgin, mature females are attracted to male odors. In olfactometer assays extracts of male bodies obtained with a two-solvent system of methanol and dichloromethane were highly attractive to virgin females. This was observed during the last two hours of the photophase, when males are sexually active, but not during the first hours of the photophase, or when mated females were tested. Extracts of male bodies obtained with diethyl ether were also attractive to virgin females, albeit not as strongly as the two-solvent extracts. These results strongly indicate that males of the olive fruit fly elicit attraction to virgin females based on olfactory stimuli. The importance of these findings for understanding the sexual behavior of the olive fruit fly is discussed.

APA, Harvard, Vancouver, ISO, and other styles

50

Kim, Young Mee, Karthika Muthuramalingam, and Moonjae Cho. "Redox Regulation of NOX Isoforms on FAK(Y397)/SRC(Y416) Phosphorylation Driven Epithelial-to-Mesenchymal Transition in Malignant Cervical Epithelial Cells." Cells 9, no. 6 (June 26, 2020): 1555. http://dx.doi.org/10.3390/cells9061555.

Full text

Abstract:

Epithelial-to-mesenchymal transition (EMT) promulgates epithelial cell associated disease-defining characteristics in tumorigenesis and organ fibrosis. Growth factors such as epidermal growth factor and fibroblast growth factor in addition to cytokines such as transforming growth factor-β1 (TGF-β1) is said to play a prominent role in remodeling related pathological events of cancer progression such as invasion, metastasis, apoptosis, EMT, etc. through redox related cellular secondary messengers, in particular the reactive oxygen species (ROS). However, the signaling cascade underlying the redox mechanism and thereby the progression of EMT remains largely unknown. In this study, upon TGF-β1 treatment, we observed an induction in NOX isoforms—NOX2 and NOX4—that have time (early and late) and cellular localization (nucleus and autophagosome co-localized) dependent effects in mediating EMT associated cell proliferation and migration through activation of the focal adhesion kinase (FAK)/SRC pathway in HeLa, human cervical cancer cells. Upon silencing NOX2/4 gene expression and using the SRC inhibitor (AZD0530), progression of TGF-β1 induced EMT related cellular remodeling, extra cellular matrix (ECM) production, cell migration and invasion, got significantly reverted. Together, these results indicate that NOX2 and NOX4 play important, albeit distinct, roles in the activation of cytokine mediated EMT and its associated processes via tyrosine phosphorylation of the FAK/SRC pathway.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Albert Ehm'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles