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Relevant bibliographies by topics / Alcalophiles

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Academic literature on the topic 'Alcalophiles'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 February 2022

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Journal articles on the topic "Alcalophiles"

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Rius, Núria, and José G. Lorén. "Buffering Capacity and Membrane H+ Conductance of Neutrophilic and Alkalophilic Gram-Positive Bacteria." Applied and Environmental Microbiology 64, no. 4 (1998): 1344–49. http://dx.doi.org/10.1128/aem.64.4.1344-1349.1998.

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ABSTRACT Buffering capacity and membrane H+ conductance were examined in three gram-positive bacteria, Staphylococcus aureus, Bacillus subtilis, and Bacillus alcalophilus. An acid pulse technique was used to measure both parameters. The buffering capacity and membrane H+conductance of B. alcalophilus are influenced by the pH of the medium and the culture conditions. Suspensions of B. alcalophilus cells from both H. A. medium andl-malate medium cultures grown at pH 10.5 exhibited higher values for these parameters than cells grown at pH 8.5. B. alcalophilus grown aerobically had a lower buffering capacity and a lower membrane conductance for protons than the neutrophilic bacteriaS. aureus and B. subtilis. Fermenting cells exhibited significantly higher values for both variables than respiring cells.

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Kondratyuk, T., and A. Kalinichenko. "Influence of essential oils and polyhexamethyleneguanidine on black yeast-like fungi Exophiala alcalophila." Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology 68, no. 3 (2014): 75–79. http://dx.doi.org/10.17721/1728_2748.2014.68.75-79.

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Influence of essential oils of the following plants Rosmarinum officinalis L., Pinus sibirica Du Tour, Abies sibirica Ledeb., Juniperus communis L., turpentine oil, and polyhexamethyleneguanidine (PHMG) on black yeast-like fungi Exophiala alcalophila FCKU 304 was investigated. Disc-diffusion method was used. Level of the antifungal influence on X. alcalophila found to be different for each chemical. 5% PHMG is characterized by the highest fungicide activity. Level of the antifungal activity of essential oils of plants Rosmarinum officinalis, Pinus sibirica and Abies sibirica is similar to 1.5-3 % PHMG activity. The PHMG and essential oil of Rosmarinum officinalis found to cause exchanges of morphometric indices of cells, colony morphology and intensity of budding E. alcalophila what is considered as adaptations of investigated culture of black yeast-like fungus. Furthermore dimorphic transition 'yeasts – mycelium' of E. alcalophila was observed under influence of essential oil of Rosmarinum officinalis.

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HOFFMANN, Artur, and Peter DIMROTH. "The electrochemical proton potential ofBacillus alcalophilus." European Journal of Biochemistry 201, no. 2 (1991): 467–73. http://dx.doi.org/10.1111/j.1432-1033.1991.tb16304.x.

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Meganathan, R., and R. Coffell. "Identity of the quinone in Bacillus alcalophilus." Journal of Bacteriology 164, no. 2 (1985): 911–13. http://dx.doi.org/10.1128/jb.164.2.911-913.1985.

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Shiomi, H. F., M. V. R. Ferreira, and I. S. Melo. "Bioprospecting bacterial strains for biological control of white mold on soybean." Scientific Electronic Archives 10, no. 2 (2017): 56–63. http://dx.doi.org/10.36560/1022017432.

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Â The search for efficient biocontrol agents in agriculture has been achieved in several pathosystems. Thus, we carried out experiments under controlled conditions in greenhouse and laboratory, involving the use of bacteria Bacillus alcalophilus, Bacillus cereus GC subgrupo B, Stenotrophomonas maltophilia, Yersinia bercovieri and Photorhabdus luminescens-luminescens obtained from biofertilizers and bovine and suine manure. The bacterial strains were multiplied in nutrient-agar medium (NA) for 48 hours to evaluate the efficiency in inhibiting the mycelial growth and the development of Sclerotinia sclerotiorum in antagonism assays in Petri dishes containing BDA medium; or in aqueous suspension adjusted in 109 ufc.mL-1 by Mac Farland scale, to seeds microbiolization and spraying the aerial parts of soybean plants, totalizing three assays. The bacterial strains BB-4 (Bacillus cereus GC subgrupo B), BS-3 (Photorhabdus luminescens-luminescens), BB-1 (Bacillus alcalophilus) and BB-6 (Yersinia bercovieri) tested were shown to be effective for inhibition of mycelial growth of the pathogen, with values between 31% and 46%, and disease development in soybean seeds and plants, with values above 50% and the same levels of control treatments with thiophanate methyl + fluazinan and thiophanate methyl. These results demonstrate the presence of promising strains in control of white mold alternatively the use of chemical fungicides

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Kondratiuk, T., and A. Kalinichenko. "Structural-functional reorganization of dimorphous black yeast-like fungi Exophiala alcalophila under influence of plant essential oils." Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology 70, no. 2 (2015): 42–46. http://dx.doi.org/10.17721/1728_2748.2015.70.42-46.

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Antifungal influence of essential oils of the following plants Cariophyllus aromaticus L., Foennicilum vulgare Mill., Lavandula anqustifolia Mill., Mentha piperita L., Melaleuca alternifolia Maid., Origanum vulgare L., Pelargonium roseum Wild., Thymus vulgaris L., and benzalconium chloride on black yeast-like fungi Exophiala alcalophila Goto et Sugiy was investigated. High level of the antifungal influence of plant oils of O. vulgare, Th. vulgaris, P. roseum and C. aromaticus was found with the usage of disc-diffusion method. The influence of plant essential oils mentioned is similar to influence of 3% benzalconium chloride. The following structural-functional reorganization of Exophiala alcalophila, i.e.: exchange of morphometric indices of cells, colony morphology, intensity of budding, dimorphous transition 'yeast-mycelium' were observed under influence of benzalconium chloride and plant essential oils of O. vulgare, Th. vulgaris, P. roseum and C. aromaticus. These exchanges illustrate wide adaptation possibilities of black yeast culture investigated.

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ZHANG, W., H. WEI, H. GAO, and G. HUANG. "Cloning and Characterization of ectABC Cluster from Bacillus alcalophilus DTY1." Chinese Journal of Biotechnology 24, no. 3 (2008): 395–400. http://dx.doi.org/10.1016/s1872-2075(08)60021-7.

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HOFFMANN, Artur, and Peter DIMROTH. "The ATPase of Bacillus alcalophilus. Reconstitution of energy-transducing functions." European Journal of Biochemistry 196, no. 2 (1991): 493–97. http://dx.doi.org/10.1111/j.1432-1033.1991.tb15841.x.

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Bala, Neeraj, Swapandeep Singh Chimni, Harvinder Singh Saini, and Bhupinder Singh Chadha. "Bacillus alcalophilus MTCC10234 catalyzed enantioselective kinetic resolution of aryl glycidyl ethers." Journal of Molecular Catalysis B: Enzymatic 63, no. 3-4 (2010): 128–34. http://dx.doi.org/10.1016/j.molcatb.2009.12.019.

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HOFFMANN, Artur, and Peter DIMROTH. "The ATPase of Bacillus alcalophilus. Purification and properties of the enzyme." European Journal of Biochemistry 194, no. 2 (1990): 423–30. http://dx.doi.org/10.1111/j.1432-1033.1990.tb15635.x.

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Dissertations / Theses on the topic "Alcalophiles"

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Tulfahi-Ebido, Ahlam. "Contribution à l'étude de la production de cyclodextrine-glycosyl-transférases par des souches alcalophiles." Compiègne, 1993. http://www.theses.fr/1993COMPD667.

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L'étude de l'isolement et de la sélection de souches bactériennes (Bacillus alcalophilus) productrices d'enzymes cyclodextrine-glycosyl-transférases (CGTases) catalysant la synthèse de cyclodextrines à partir d'amidon, s'inscrit dans un contexte de microbiologie appliquée d'un grand intérêt industriel. L'étude sur la sélection de souches productrices de CGTases issues des collections internationales, a permis dans un premier temps de retrouver les capacités des souches qualifiées d'industrielles et d'étudier l'influence de la composition du milieu sur les activités enzymatiques en fioles et en fermenteur. Des mutants hyperproducteurs de CGTases ont ensuite été obtenus grâce à la mise en œuvre de la méthode de mutagénèse par rayonnement ultra-violet et la mise au point de différentes techniques de criblage. L'analyse de mille cinq cent mutants a conduit à la sélection de treize clones génétiquement stables et caractérisés par une activité alpha, beta ou gamma CGTase élevée. Par rapport à la souche de référence, quelques clones présentent une activité alpha CGTase multipliée par facteur 9, beta CGTase multipliée par facteur 2 et gamma CGTase multipliée par facteur 4. Pour le meilleur clone, la somme des trois activités est multipliée par 4. Par ailleurs, des souches sauvages ont été isolées à partir d'un biotope naturel : une terre alcaline provenant d'un champs de blé traité aux pesticides s'est révélée très intéressante pour la recherche de souches hyperproductrices de CGTases. De plus, une méthode de criblage utilisant des milieux sélectifs à base de alpha ou gamma cyclodextrine s'est avérée particulièrement efficace. Ces essais nous ont permis d'isoler six souches dont la capacité de production est supérieure à celle de la souche témoin. La souche la plus active présente une activité CGTase globale trois fois plus importante que la souche de référence. Ainsi, le choix d'un écosystème particulier associé à une procédure de sélection spécifique a permis d'atteindre une probabilité de sélection de l'ordre de 1/10, alors que les méthodes traditionnelles ne permettent pas de dépasser 1/100 à 1/1000 pour Bacillus alcalophilus.

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Ben, Aissa Fatma. "Ecologie microbienne des systèmes hydrothermaux marins alcalins de la baie de Prony (Nouvelle-Calédonie)." Thesis, Aix-Marseille, 2015. http://www.theses.fr/2015AIXM4760.

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Nous avons étudié l’écologie microbienne d’un site hydrothermal alcalin sous-marin peu profond (0-50 m) localisé dans la baie de Prony (PHF) dans le sud de Nouvelle-Calédonie (Pacifique Sud-Ouest) comparable par son fonctionnement au site hydrothermal alcalin profond (800m) de Lost City (LCHF) (Dorsale médio-atlantique). PHF au même titre que LCHF est un système hydrothermal ultramafique associé à des réactions de serpentinisation des roches du manteau terrestre, libérant des fluides anoxiques très alcalins (jusqu'à pH 11) riche en calcium, en hydrogène et en méthane dissous. Le site hydrothermal de Prony (PHF) est caractérisé par de grandes cheminées de carbonate émettant des fluides dont la température n’excède pas 40°C. Les approches moléculaires ont démontré une prédominance des bactéries (Firmicutes, Deltaproteobacteria …) sur les archées (Methanosarcinales). En ce qui concerne les mises en culture, elles sont restées vaines pour la plupart des grands groupes trophiques recherchés (bactéries sulfato-réductrices, archées méthanogènes) excepté pour les fermentaires relevant du phylum des Firmicutes. Deux nouvelles bactéries appartenant à ce phylum ont été isolées. Il s’agit (i) de Vallitalea pronyensis vraisemblablement associée aux cheminées hydrothermales et (ii) d’Alkaliphilus hydrothermalis qui serait plutôt indigène aux fluides alcalins émis par les cheminées si l’on en juge par ses conditions physico-chimiques optimales de croissance en adéquation avec celles des fluides. Ces deux microorganismes représentent les premiers anaérobies stricts isolés de systèmes hydrothermaux alcalins serpentinisés à ce jour<br>We studied the microbial ecology of an alkaline hydrothermal submarine shallow field (0-50 m) located in Prony Bay (PHF) in the south of New Caledonia (SW Pacific) similar to the deep alkaline hydrothermal site (800m) of Lost City (LCHF) (Mid-Atlantic Ridge). Similarly to LCHF, PHF is an ultramafic hydrothermal system functioning on the basis of serpentinization reactions of the mantle rocks, releasing anoxic, highly alkaline fluids (to pH 11) rich in calcium, and in dissolved hydrogen and methane. The Prony hydrothermal field (PHF) is characterized by large carbonate chimneys emitting fluids with temperatures not exceeding 40 °C. Molecular approaches revealed a prevalence of Bacteria (Firmicutes, Deltaproteobacteria…) over Archaea (Methanosarcinales). Regarding microbial cultures, they were unsuccessful for most major trophic groups (sulfate-reducing bacteria, methanogens) with the exception of fermentative representatives of the phylum Firmicutes. Two novel bacteria belonging to this phylum were isolated. They include (i) Vallitalea pronyensis which is likely associated with hydrothermal vents and (ii) Alkaliphilus hydrothermalis which should be indigenous to alkaline fluids emitted from chimneys since its growth optimal physicochemical conditions match those of fluids. These two bacteria represent the first anaerobic microorganisms isolated from alkaline hydrothermal serpentinized systems so far

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Blanco, Kate Cristina [UNESP]. "Produção de ciclodextrina glicosiltransferase por Bacillus sp subgrupo alcalophilus: otimização por planejamento experimental." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/94973.

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Made available in DSpace on 2014-06-11T19:27:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-06-10Bitstream added on 2014-06-13T20:35:51Z : No. of bitstreams: 1 blanco_kc_me_rcla.pdf: 835926 bytes, checksum: 46db69ad433dc1b354710697a08da977 (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>Este trabalho tem como objetivo estudar a produção da enzima ciclodextrina glicosiltransferase (CGTase) empregando culturas de Bacillus sp subgrupo alcalophilus utilizando como fonte de carbono fécula de mandioca (polvilho) proveniente de uma fecularia de mandioca. Nos ensaios foram empregados Bacillus sp subgrupo alcalophilus, isolado de água residuária de uma fecularia de mandioca. Para avaliar a produção de CGTase mediante a atividade enzimática pelo Bacillus sp subgrupo alcalophilus foi utilizado um planejamento fatorial a dois (2) níveis, estudando como variáveis as concentrações da fonte de carbono (polvilho), de nitrogênio e de carbonato de sódio. Os experimentos foram realizados em Erlenmeyers de 300 mL de capacidade contendo 100 mL do meio de produção com pH inicial de 9,2, a 150 rpm e temperatura de 35 ± 1ºC durante 72 horas de fermentação. A produção de CGTase foi monitorada pela determinação da atividade enzimática (U/mL). Após os ensaios realizados em frascos foram realizados experimentos em biorreator de 5 L de volume útil, contendo 2 L do meio de produção, pH de 9,20, agitação de 150 rpm e temperatura de 35 ± 1ºC durante 72 h de fermentação, utilizando um planejamento fatorial a dois (2) níveis, estudando como variáveis as taxas de aeração e a velocidade de agitação. A otimização das concentrações da fonte de carbono, nitrogênio e carbonato de sódio foram obtidas a partir de um planejamento experimental composto central (PCC) e seus resultados analisados pelas superfícies de resposta. Os melhores resultados do planejamento encontrados no ponto central, corresponderam a 6,96 g/L da fonte de carbono, 8,07 g/L de nitrogênio e 9,45 g/L de carbonato de sódio. A maior produtividade obtida de CGTase após 72 horas de fermentação, foi 98,86 U/mL com valor teórico de 98,87 U/mL. A partir do melhor resultado obtido no PCC, determinou-se...<br>The aim of this study was to investigate the Cyclodextrin glycosyltransferase (CGTase) enzyme production by Bacillus sp subgroup alcalophilus using cassava starch (manioc flour) as a carbon source. Bacillus sp subgroup alcalophilus was isolated from wastewater of cassava flour industry. To evaluate the assays results, two (2) levels of complete factorial experimental design was used, studying the variables: carbon source, nitrogen and sodium carbonate concentrations. The experiments were performed in 300 mL erlenmeyer flasks containing 100 mL of medium production with initial pH of 9.2, at 150 rpm and 35±1ºC, during 72 hour. CGTase production was monitored by measurements of enzymatic activity (U/mL). After the flasks experiments, assays was running in 5 L containing 2 L of medium production, pH 9.2, 35 ± 1ºC during 72-hours using a factorial design at two (2) levels for aeration and agitation rate. The optimization of carbon source, nitrogen and sodium carbonate concentrations was obtained by a central composite design and their results analyzed by surface response. The best results was located on the central point, 6.96 g/L of carbon source, 8.07 g/L of nitrogen and 9.45 g/L of sodium carbonate. The CGTase activity predicted by model was 98.86 U/mL and the experimental activity obtained was 98,87 U/mL. After the best results obtained by PCC, the conditions of aeration (vvm) and agitation (rpm) rates was determined in bioreactor using a complete factorial experimental design. The best conditions, of 2.18 vvm and agitation of 157.07 rpm gave a experimental predicted CGTase activity of 130.36 U/mL, which was very close to the theoric CGTase activity of 130.33 U/mL.

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4

Blanco, Kate Cristina. "Produção de ciclodextrina glicosiltransferase por Bacillus sp subgrupo alcalophilus : otimização por planejamento experimental /." Rio Claro : [s.n.], 2009. http://hdl.handle.net/11449/94973.

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Resumo: Este trabalho tem como objetivo estudar a produção da enzima ciclodextrina glicosiltransferase (CGTase) empregando culturas de Bacillus sp subgrupo alcalophilus utilizando como fonte de carbono fécula de mandioca (polvilho) proveniente de uma fecularia de mandioca. Nos ensaios foram empregados Bacillus sp subgrupo alcalophilus, isolado de água residuária de uma fecularia de mandioca. Para avaliar a produção de CGTase mediante a atividade enzimática pelo Bacillus sp subgrupo alcalophilus foi utilizado um planejamento fatorial a dois (2) níveis, estudando como variáveis as concentrações da fonte de carbono (polvilho), de nitrogênio e de carbonato de sódio. Os experimentos foram realizados em Erlenmeyers de 300 mL de capacidade contendo 100 mL do meio de produção com pH inicial de 9,2, a 150 rpm e temperatura de 35 ± 1ºC durante 72 horas de fermentação. A produção de CGTase foi monitorada pela determinação da atividade enzimática (U/mL). Após os ensaios realizados em frascos foram realizados experimentos em biorreator de 5 L de volume útil, contendo 2 L do meio de produção, pH de 9,20, agitação de 150 rpm e temperatura de 35 ± 1ºC durante 72 h de fermentação, utilizando um planejamento fatorial a dois (2) níveis, estudando como variáveis as taxas de aeração e a velocidade de agitação. A otimização das concentrações da fonte de carbono, nitrogênio e carbonato de sódio foram obtidas a partir de um planejamento experimental composto central (PCC) e seus resultados analisados pelas superfícies de resposta. Os melhores resultados do planejamento encontrados no ponto central, corresponderam a 6,96 g/L da fonte de carbono, 8,07 g/L de nitrogênio e 9,45 g/L de carbonato de sódio. A maior produtividade obtida de CGTase após 72 horas de fermentação, foi 98,86 U/mL com valor teórico de 98,87 U/mL. A partir do melhor resultado obtido no PCC, determinou-se... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: The aim of this study was to investigate the Cyclodextrin glycosyltransferase (CGTase) enzyme production by Bacillus sp subgroup alcalophilus using cassava starch (manioc flour) as a carbon source. Bacillus sp subgroup alcalophilus was isolated from wastewater of cassava flour industry. To evaluate the assays results, two (2) levels of complete factorial experimental design was used, studying the variables: carbon source, nitrogen and sodium carbonate concentrations. The experiments were performed in 300 mL erlenmeyer flasks containing 100 mL of medium production with initial pH of 9.2, at 150 rpm and 35±1ºC, during 72 hour. CGTase production was monitored by measurements of enzymatic activity (U/mL). After the flasks experiments, assays was running in 5 L containing 2 L of medium production, pH 9.2, 35 ± 1ºC during 72-hours using a factorial design at two (2) levels for aeration and agitation rate. The optimization of carbon source, nitrogen and sodium carbonate concentrations was obtained by a central composite design and their results analyzed by surface response. The best results was located on the central point, 6.96 g/L of carbon source, 8.07 g/L of nitrogen and 9.45 g/L of sodium carbonate. The CGTase activity predicted by model was 98.86 U/mL and the experimental activity obtained was 98,87 U/mL. After the best results obtained by PCC, the conditions of aeration (vvm) and agitation (rpm) rates was determined in bioreactor using a complete factorial experimental design. The best conditions, of 2.18 vvm and agitation of 157.07 rpm gave a experimental predicted CGTase activity of 130.36 U/mL, which was very close to the theoric CGTase activity of 130.33 U/mL.<br>Orientador: Antonio Carlos Simões Pião<br>Coorientador: Jonas Contiero<br>Banca: Heloiza Ferreira Alves do Prado<br>Banca: Sandra Regina Ceccato Antonini<br>Mestre

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Book chapters on the topic "Alcalophiles"

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Brünger, Axel T., Yasmin Karimi-Nejad, Frans A. A. Mulder, John R. Martin, Dick Schipper, and Rolf Boelens. "NMR Studies of the 269 Residue Serine Protease PB92 from Bacillus Alcalophilus." In NMR in Supramolecular Chemistry. Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4615-9_14.

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Conference papers on the topic "Alcalophiles"

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Cheng, Kun, Fuping Lu, and Xiaomei Liang. "An Effective Electroporation Protocol for Bacillus alcalophilus TCCC11004." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5515931.

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Chen, Kun, Yong Jiang, Nan Wang, Xuegang Luo, Fuping Lu, and Tongcun Zhang. "Cloning, Characterization and Application of the Promoter Region of the Alkaline Protease Gene in Bacillus alcalophillus PB92." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5162515.

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