Academic literature on the topic 'Alcanivorax'
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Journal articles on the topic "Alcanivorax"
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Liao, Xianzhi, Qiliang Lai, Junpeng Yang, Chunming Dong, Dengfeng Li, and Zongze Shao. "Alcanivorax sediminis sp. nov., isolated from deep-sea sediment of the Pacific Ocean." International Journal of Systematic and Evolutionary Microbiology 70, no. 7 (July 1, 2020): 4280–84. http://dx.doi.org/10.1099/ijsem.0.004285.
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A taxonomic study was carried out on strain PA15-N-34T, which was isolated from deep-sea sediment of Pacific Ocean. The bacterium was Gram-stain-positive, oxidase- and catalase-positive and rod-shaped. Growth was observed at salinity of 0–15.0% NaCl and at temperatures of 10–45 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PA15-N-34T belonged to the genus Alcanivorax , with the highest sequence similarity to Alcanivorax profundi MTEO17T (97.7 %), followed by Alcanivorax nanhaiticus 19 m-6T (97.3 %) and 12 other species of the genus Alcanivorax (93.4 %–97.0 %). The average nucleotide identity and DNA–DNA hybridization values between strain PA15-N-34T and type strains of the genus Alcanivorax were 71.46–81.78% and 18.7–25.2 %, respectively. The principal fatty acids (>10 %) were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c; 31.2 %), C16 : 0 (25.0 %) and summed feature 3 (14.6 %). The DNA G+C content was 57.15 mol%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, four unidentified aminolipids and three unidentified lipids. The novel strain can be differentiated from its closest type strain by a negative test for urease and the presence of diphosphatidylglycerol and aminolipid. The combined genotypic and phenotypic data show that strain PA15-N-34T represents a novel species within the genus Alcanivorax , for which the name Alcanivorax sediminis sp. nov. is proposed, with the type strain PA15-N-34T (=MCCC 1A14738T=KCTC 72163T).
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Liu, Chenli, and Zongze Shao. "Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment." International Journal of Systematic and Evolutionary Microbiology 55, no. 3 (May 1, 2005): 1181–86. http://dx.doi.org/10.1099/ijs.0.63443-0.
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Two bacterial strains, B-5T and NO1A, were isolated from the surface water of the Bohai Sea and deep-sea sediment of the east Pacific Ocean, respectively. Both strains were halophilic, aerobic, Gram-negative, non-spore-forming, catalase- and oxidase-positive motile rods. They grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. On the basis of 16S rRNA gene sequence similarity, strains B-5T and NO1A were shown to belong to the γ-Proteobacteria. Highest similarity values were found with Alcanivorax venustensis (95·2 %), Alcanivorax jadensis (94·6 %) and Alcanivorax borkumensis (94·1 %). Principal fatty acids of both strains were C16 : 0, C16 : 1 ω7c and C18 : 1 ω7c. The chemotaxonomically characteristic fatty acid C19 : 0 cyclo ω8c was also detected. On the basis of the above, together with results of physiological and biochemical tests, DNA–DNA hybridization, comparisons of 16S–23S internal transcribed spacer sequences and comparisons of the partial deduced amino acid sequence of alkane hydroxylase, both strains were affiliated to the genus Alcanivorax but were differentiated from recognized Alcanivorax species. Therefore, a novel species, Alcanivorax dieselolei sp. nov., represented by strains B-5T and NO1A is proposed, with the type strain B-5T (=DSM 16502T=CGMCC 1.3690T).
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Kyoung Kwon, Kae, Ji Hye Oh, Sung-Hyun Yang, Hyun-Seok Seo, and Jung-Hyun Lee. "Alcanivorax gelatiniphagus sp. nov., a marine bacterium isolated from tidal flat sediments enriched with crude oil." International Journal of Systematic and Evolutionary Microbiology 65, Pt_7 (July 1, 2015): 2204–8. http://dx.doi.org/10.1099/ijs.0.000244.
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A Gram-reaction-negative, rod-shaped marine bacterium, designated MEBiC08158T, was isolated from sediments collected from Taean County, Korea, near the Hebei Spirit tanker oil spill accident. 16S rRNA gene sequence analysis revealed that strain MEBiC08158T was closely related to Alcanivorax marinus R8-12T (99.5 % similarity) but was distinguishable from other members of the genus Alcanivorax (93.7–97.1 %). The DNA–DNA hybridization value between strain MEBiC08158T and A. marinus R8-12T was 58.4 %. Growth of strain MEBiC08158T was observed at 15–43 °C (optimum 37–40 °C), at pH 6.0–9.5 (optimum pH 7.0–8.0) and with 0.5–16 % NaCl (optimum 1.5–3.0 %). The dominant fatty acids were C16 : 0, C19 : 0 cyclo ω8c, C12 : 0, C18 : 1ω7c, C12 : 0 3-OH and summed feature 3 (comprising C15 : 0 2-OH and/or C16 : 1ω7c). Several phenotypic characteristics differentiate strain MEBiC08158T from phylogenetically close members of the genus Alcanivorax. Therefore, strain MEBiC08158T should be classified as representing a novel species of the genus Alcanivorax, for which the name Alcanivorax gelatiniphagus sp. nov. is proposed. The type strain is MEBiC08158T ( = KCCM 42990T = JCM 18425T).
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Lai, Qiliang, Jianning Wang, Li Gu, Tianling Zheng, and Zongze Shao. "Alcanivorax marinus sp. nov., isolated from deep-sea water." International Journal of Systematic and Evolutionary Microbiology 63, Pt_12 (December 1, 2013): 4428–32. http://dx.doi.org/10.1099/ijs.0.049957-0.
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A taxonomic study was carried out on strain R8-12T, which was isolated from deep-sea water of the Indian Ocean during the screening of oil-degrading bacteria. The isolate was Gram-stain-negative, oxidase and catalase-positive. Growth was observed at salinities from 0.5 to 15 % (optimum 3 %), at pH from 6–10 (optimum 7–8) and at temperatures from 10 to 42 °C (optimum 28 °C). On the basis of 16S rRNA gene sequence similarity, strain R8-12T was shown to belong to the genus Alcanivorax and to be related to Alcanivorax venustensis DSM 13974T (97.2 %), A. dieselolei B-5T (95.0 %), A. balearicus MACL04T (94.6 %), A. hongdengensis A-11-3T (94.3 %), A. jadensis T9T (93.8 %), A. borkumensis SK2T (93.7 %) and A. pacificus W11-5T (93.7 %). The gyrB sequence similarities between R8-12T and other species of the genus Alcanivorax ranged from 77.9 % to 86.9 %. The major fatty acids were C16 : 0 (31.8 %), C18 : 1ω7c (20.3 %), C19 : 0ω8c cyclo (15.8 %) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) (8.9 %). The polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two aminolipids (AL1–AL2) and two phospholipids (PL1–PL2). Three alkane hydroxylase (alkB) genes were identified in the genome. The G+C content of the chromosomal DNA was 66.1 mol%. DNA–DNA hybridization showed that strain R8-12T and A. venustensis DSM 13974T had a DNA–DNA relatedness of 63±3 %. According to its phenotypic features and fatty acid composition as well as the 16S rRNA and gyrB gene sequences, the novel strain represents a member of the genus Alcanivorax , but could be easily distinguished from all other known species of the genus Alcanivorax described to date. The name Alcanivorax marinus sp. nov. is proposed, with the type strain R8-12T ( = MCCC 1A00382T = LMG 24621T = CCTCC AB 208234T).
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Lai, Qiliang, Liping Wang, Yuhui Liu, Yuanyuan Fu, Huanzi Zhong, Baojiang Wang, Liang Chen, Jianning Wang, Fengqin Sun, and Zongze Shao. "Alcanivorax pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium." International Journal of Systematic and Evolutionary Microbiology 61, no. 6 (June 1, 2011): 1370–74. http://dx.doi.org/10.1099/ijs.0.022368-0.
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A taxonomic study was carried out on a novel bacterial strain, designated W11-5T, which was isolated from a pyrene-degrading consortium enriched from deep-sea sediment of the Pacific Ocean. The isolate was Gram-reaction-negative and oxidase- and catalase-positive. Growth was observed in 0.5–12 % (w/v) NaCl and at 10–42 °C. On the basis of 16S rRNA gene sequence analysis, strain W11-5T was shown to belong to the genus Alcanivorax with a close relation to A. dieselolei B-5T (93.9 % 16S rRNA sequence similarity), A. balearicus MACL04T (93.1 %), A. hongdengensis A-11-3T (93.1 %), A. borkumensis SK2T (93.0 %), A. venustensis ISO4T (93.0 %) and A. jadensis T9T (92.9 %). Similarities between the gyrB gene sequences of W11-5T and other species of the genus Alcanivorax were between 76.8 and 80.8 %. The principal fatty acids were C12 : 0 3-OH (8.0 %), C16 : 0 (29.1 %) and C18 : 1ω7c (27.4 %). The G+C content of the chromosomal DNA was 60.8 mol%. Based on its morphology, physiology and fatty acid composition as well as the results of 16S rRNA and gyrB gene sequence analyses, strain W11-5T ( = MCCC 1A00474T = CCTCC AB 208236T = LMG 25514T) represents a novel species of the genus Alcanivorax, for which the name Alcanivorax pacificus sp. nov. is proposed.
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Rahul, K., Ch Sasikala, L. Tushar, R. Debadrita, and Ch V. Ramana. "Alcanivorax xenomutans sp. nov., a hydrocarbonoclastic bacterium isolated from a shrimp cultivation pond." International Journal of Systematic and Evolutionary Microbiology 64, Pt_10 (October 1, 2014): 3553–58. http://dx.doi.org/10.1099/ijs.0.061168-0.
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Two bacterial strains (JC109T and JC261) were isolated from a sediment sample collected from a shrimp cultivation pond in Tamil Nadu (India). Cells were Gram-stain-negative, motile rods. Both strains were positive for catalase and oxidase, hydrolysed Tween 80, and grew chemo-organoheterotrophically with an optimal pH of 6 (range pH 4–9) and at 30 °C (range 25–40 °C). Based on 16S rRNA gene sequence analysis, strains JC109T and JC261 were identified as belonging to the genus Alcanivorax with Alcanivorax dieselolei B-5T (sequence similarity values of 99.3 and 99.7 %, respectively) and Alcanivorax balearicus MACL04T (sequence similarity values of 98.8 and 99.2 %, respectively) as their closest phylogenetic neighbours. The 16S rRNA gene sequence similarity between strains JC109T and JC261 was 99.6 %. The level of DNA–DNA relatedness between the two strains was 88 %. Strain JC109T showed 31±1 and 26±2 % DNA–DNA relatedness with A. dieselolei DSM 16502T and A. balearicus DSM 23776T, respectively. The DNA G+C content of strains JC109T and JC261 was 54.5 and 53.4 mol%, respectively. Polar lipids of strain JC109T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids. The major fatty acids were C10 : 0, C12 : 0, C16 : 0, C12 : 0 3-OH, C16 : 1ω7c, C18 : 1ω7c and C19 : 0 cyclo ω8c. Both strains could utilize diesel oil and a variety of xenobiotics as carbon and energy sources. The results of physiological, biochemical, chemotaxonomic and molecular analyses allowed the clear differentiation of strains JC109T and JC261 from all other members of the genus Alcanivorax . Strains JC109T and JC261 are thus considered to represent a novel species, for which the name Alcanivorax xenomutans sp. nov. is proposed. The type strain is JC109T ( = KCTC 23751T = NBRC 108843T).
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Manilla-Pérez, Efraín, Christina Reers, Meike Baumgart, Stephan Hetzler, Rudolf Reichelt, Ursula Malkus, Rainer Kalscheuer, Marc Wältermann, and Alexander Steinbüchel. "Analysis of Lipid Export in Hydrocarbonoclastic Bacteria of the Genus Alcanivorax: Identification of Lipid Export-Negative Mutants of Alcanivorax borkumensis SK2 and Alcanivorax jadensis T9." Journal of Bacteriology 192, no. 3 (November 20, 2009): 643–56. http://dx.doi.org/10.1128/jb.00700-09.
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ABSTRACT Triacylglycerols (TAGs), wax esters (WEs), and polyhydroxyalkanoates (PHAs) are the major hydrophobic compounds synthesized in bacteria and deposited as cytoplasmic inclusion bodies when cells are cultivated under imbalanced growth conditions. The intracellular occurrence of these compounds causes high costs for downstream processing. Alcanivorax species are able to produce extracellular lipids when the cells are cultivated on hexadecane or pyruvate as the sole carbon source. In this study, we developed a screening procedure to isolate lipid export-negative transposon-induced mutants of bacteria of the genus Alcanivorax for identification of genes required for lipid export by employing the dyes Nile red and Solvent Blue 38. Three transposon-induced mutants of A. jadensis and seven of A. borkumensis impaired in lipid secretion were isolated. All isolated mutants were still capable of synthesizing and accumulating these lipids intracellularly and exhibited no growth defect. In the A. jadensis mutants, the transposon insertions were mapped in genes annotated as encoding a putative DNA repair system specific for alkylated DNA (Aj17), a magnesium transporter (Aj7), and a transposase (Aj5). In the A. borkumensis mutants, the insertions were mapped in genes encoding different proteins involved in various transport processes, like genes encoding (i) a heavy metal resistance (CZCA2) in mutant ABO_6/39, (ii) a multidrug efflux (MATE efflux) protein in mutant ABO_25/21, (iii) an alginate lyase (AlgL) in mutants ABO_10/30 and ABO_19/48, (iv) a sodium-dicarboxylate symporter family protein (GltP) in mutant ABO_27/29, (v) an alginate transporter (AlgE) in mutant ABO_26/1, or (vi) a two-component system protein in mutant ABO_27/56. Site-directed MATE, algE, and algL gene disruption mutants, which were constructed in addition, were also unable to export neutral lipids and confirmed the phenotype of the transposon-induced mutants. The putative localization of the different gene products and their possible roles in lipid excretion are discussed. Beside this, the composition of the intra- and extracellular lipids in the wild types and mutants were analyzed in detail.
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Puspitasari, Ita, Agus Trianto, and Jusup Supriyanto. "Eksplorasi Bakteri Pendegradasi Minyak dari Perairan Pelabuhan Tanjung Mas, Semarang." Journal of Marine Research 9, no. 3 (July 16, 2020): 281–88. http://dx.doi.org/10.14710/jmr.v9i3.27606.
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ABSTRAK: Tanjung Mas merupakan salah satu kawasan pelabuhan di Jawa Tengah yang terus mengalami peningkatan jumlah kapal setiap tahunnya. Hal ini berimbas pada semakin meningkatnya tumpahan minyak solar yang masuk ke perairan. Upaya yang bisa dilakukan untuk mengurangi jumlah tumpahan minyak solar di Pelabuhan Tanjung Mas yaitu dengan melakukan penelitian bioremediasi menggunakan bakteri indigenouse. Tujuan penelitian ini dilakukan yaitu mendapatkan isolat bakteri air laut asal Pelabuhan Tanjung Mas, Semarang yang mampu mendegradasi minyak solar dan mengetahui kemampuan masing-masing isolat untuk mendegradasi minyak solar. Penelitian ini dilaksanakan pada bulan Juli sampai Oktober 2019 dengan pengambilan sample air laut menggunakan metode purpossive sampling, metode isolasi menggunakan pour plate, metode uji minyak secara gravimetri dan pertumbuhan bakteri menggunakan Standart Plate Count (SPC). Hasil isolasi yaitu didapatkan 2 isolat unggul yaitu bakteri Alcanivorax nanhaiticus dan Halomonas meridiana. Bakteri Alcanivorax nanhaiticus mampu mendegradasi 54% minyak solar dan Halomonas meridiana mampu mendegradasi 72% minyak solar. Kedua bakteri merupakan bakteri hidrokarbonoklastik atau bakteri yang memiliki kemampuan mendegradasi minyak solar (hidrokarbon) karena mengandung enzim monooksigenase. Dari penelitian ini dapat disimpulkan bahwa di Pelabuhan Tanjung Mas terdapat bakteri yang mampu mendegradasi minyak solar yaitu Alcanivorax nanhaiticus dan Halomonas meridiana. Kedua isolat mampu mendegradasi lebih dari 50% minyak solar yang diujikan. ABSTRACT: Tanjung Mas is one of the port areas in Central Java that continues to experience an increase in the number of ships each year. This has an impact on the increasing amount of diesel fuel spills that enter the waters. Efforts that can be made to reduce the number of diesel fuel spills in the Port of Tanjung Mas is to conduct bioremediation research using indigenous bacteria. The purpose of this study was to obtain seawater bacterial isolates from the Port of Tanjung Mas, Semarang that can degrade diesel fuel and determine the ability of each isolate to degrade diesel fuel. This research was conducted in July to October 2019 by taking seawater samples using a purposive sampling method, isolation method using pour plates, gravimetric fuel test methods and bacterial growth using Standard Plate Count (SPC). The results of the isolation were obtained 2 superior isolates namely Alcanivorax nanhaiticus and Halomonas meridiana. Alcanivorax nanhaiticus can degrade 54% of diesel fuel and Halomonas meridiana can degrade 72% of diesel fuel. Both bacteria are hydrocarbonoclastic bacteria or bacteria that can degrade diesel oil (hydrocarbons) because they contain the enzyme monooxsigenase. From this study, it can be concluded that at Tanjung Mas Port there are bacteria that can degrade diesel fuel, namely Alcanivorax nanhaiticus and Halomonas meridiana. Both isolates were able to degrade more than 50% of the tested diesel fuel.
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Teramoto, Maki, Masahito Suzuki, Fumiyoshi Okazaki, Ariani Hatmanti, and Shigeaki Harayama. "Oceanobacter-related bacteria are important for the degradation of petroleum aliphatic hydrocarbons in the tropical marine environment." Microbiology 155, no. 10 (October 1, 2009): 3362–70. http://dx.doi.org/10.1099/mic.0.030411-0.
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Petroleum-hydrocarbon-degrading bacteria were obtained after enrichment on crude oil (as a ‘chocolate mousse’) in a continuous supply of Indonesian seawater amended with nitrogen, phosphorus and iron nutrients. They were related to Alcanivorax and Marinobacter strains, which are ubiquitous petroleum-hydrocarbon-degrading bacteria in marine environments, and to Oceanobacter kriegii (96.4–96.5 % similarities in almost full-length 16S rRNA gene sequences). The Oceanobacter-related bacteria showed high n-alkane-degrading activity, comparable to that of Alcanivorax borkumensis strain SK2. On the other hand, Alcanivorax strains exhibited high activity for branched-alkane degradation and thus could be key bacteria for branched-alkane biodegradation in tropical seas. Oceanobacter-related bacteria became most dominant in microcosms that simulated a crude oil spill event with Indonesian seawater. The dominance was observed in microcosms that were unamended or amended with fertilizer, suggesting that the Oceanobacter-related strains could become dominant in the natural tropical marine environment after an accidental oil spill, and would continue to dominate in the environment after biostimulation. These results suggest that Oceanobacter-related bacteria could be major degraders of petroleum n-alkanes spilt in the tropical sea.
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Cappello, Simone, Ilaria Corsi, Sabrina Patania, Elisa Bergami, Maurizio Azzaro, Monique Mancuso, Maria Genovese, Alessia Lunetta, and Gabriella Caruso. "Characterization of Five Psychrotolerant Alcanivorax spp. Strains Isolated from Antarctica." Microorganisms 11, no. 1 (December 24, 2022): 58. http://dx.doi.org/10.3390/microorganisms11010058.
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Five psychrotolerant Alcanivorax spp. strains were isolated from Antarctic coastal waters. Strains were screened for molecular and physiological properties and analyzed regarding their growth capacity. Partial 16S rDNA, alk-B1, and P450 gene sequencing was performed. Biolog EcoPlates and the API 20E test were used to evaluate metabolic and biochemical profiles. Bacterial growth in sodium acetate was determined at 4, 15, 20, and 25 °C to evaluate the optimal temperature. Furthermore, the ability of each strain to grow in a hydrocarbon mixture at 4 and 25 °C was assayed. Biosurfactant production tests (drop-collapse and oil spreading) and emulsification activity tests (E24) were also performed. Concerning results of partial gene sequencing (16S rDNA, alk-B1, and P450), a high similarity of the isolates with the same genes isolated from other Alcanivorax spp. strains was observed. The metabolic profiles obtained by Biolog assays showed no significant differences in the isolates compared to the Alcanivorax borkumensis wild type. The results of biodegradative tests showed their capability to grow at different temperatures. All strains showed biosurfactant production and emulsification activity. Our findings underline the importance to proceed in the isolation and characterization of Antarctic hydrocarbon-degrading bacterial strains since their biotechnological and environmental applications could be useful even for pollution remediation in polar areas.
Dissertations / Theses on the topic "Alcanivorax"
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Hu, Chen-Yi, and 胡朕溢. "Growth and diesel degradation of Alcanivorax sp. under various temperatures." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/5jk3a2.
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碩士國立臺灣海洋大學
環境生物與漁業科學學系
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This study cellected sediments and water samples from Badouzi Fishing Port for isolating strains in diesel agar. The selected strain was characterized by characterization and 16S rDNA, then cultivating in different diesel concentrations (1, 2, 5 and 10%) and temperature conditions (20, 25 and 30°C). Observing changes in bacterial biomass and degradation of diesel.According to the characteristic and sequencing, the strain was confirmed to be Alcanivorax sp.. The bacterial biomass in diesel were significant higher than control group (p<0.05). The growth rate of bacteria cultured in 5% diesel was significant higher than the other treatments after 6 days, and the growth rate of bacteria at 10% diesel concentration was slower the other treatments. The degradation rate of 5% diesel concentration was the highest during the experiment period, followed by 2% and 1% concentration, and the degradation under 10% diesel concentration was not obviously.The amount of bacteria under different temperature conditions began to differ significantly after 3 days (p<0.05). The bacterial growth rate was the highest at 30°C, and there are no significant growth difference at 20°C. After 7 days of experiment, the highest degradation rate was found at 30°C. The diesel concentration at different temperatures had significanty difference after 4 days (p<0.05). This results showed the degradation rate of diesel was significant increased with ambient temperature (p<0.05).
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Kashim, Zainab Abimbola. "Genomic context analytics of genes for universal stress proteins from petroleum-degrading Alcanivorax." Diss., 2016. http://hdl.handle.net/10500/23365.
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Alcanivorax species are gram negative bacteria that usually require aliphatic hydrocarbon
as the sole carbon source for growth. The ability to use petroleum in polluted environments as
energy source makes Alcanivorax species biotechnologically relevant in bioremediation.
Universal stress proteins confer ability to respond to unfavourable environments, thus the present
study was done to analyse the genomic context of genes for universal stress proteins in
Alcanivorax genomes. A combination of bioinformatics and visual analytics approaches were
used to analyze genome-enabled data including sequences and gene expression datasets. On the
basis of transcription unit and adjacent genes, two types of Alcanivorax USP genes observed
were (i) adjacent to cyclic nucleotide-binding and oxygen sensing functions; and (ii) adjacent to
sulfate transporter function. Both types of genes encode two universal stress protein domains
(pfam00582) also referred to as tandem-type universal stress proteins. The sequence and structural characteristics of each of the four USP domains in Alcanivorax needs to be further
investigated. This dissertation research evaluated data from Alcanivorax borkumensis cells
(grown on either pyruvate or hexadecane as carbon source) that were stressed with 1-octanol and
data collected at 15 min, 30 min, 60 min and 90 min after 1-octanol addition. The two genes for
Alcanivorax borkumensis SK2 universal stress proteins, ABO_1340 and ABO_1511, had the
same direction of expression for adjacent genes. A limitation of this research was that findings
based on bioinformatics and visual analytics methods may need confirmation by molecular
methods. The differences observed may also reflect the quality of the annotations provided for
genes. The sequence and structural characteristics of each of the four USP domains in
Alcanivorax needs to be further investigated. Further research is needed on the relationship
between number, length and order of genes in operons that include genes for universal stress proteins. Additionally, in vitro studies to confirm the functional prediction made from the
genomic context of the universal stress protein in Alcanivorax genome. The knowledge
discovered from this genome context analytics research could contribute to improving the
performance of Alcanivorax species in bioremediation of environments polluted with petroleumCollege of Agriculture and Environmental Sciences
M. Sc. (Environmental Science)
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Bianchi, Patrick. "Characterization of Bacterial Hydrocarbon Degradation Potential in the Red Sea Through Metagenomic and Cultivation Methods." Diss., 2018. http://hdl.handle.net/10754/627295.
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Prokaryotes are the main actors in biogeochemical cycles that are fundamental in global nutrient cycling. The characterization of microbial communities and isolates can enhance the comprehension of such cycles. Potentially novel biochemical processes can be discovered in particular environments with unique characteristics. The Red Sea can be considered as a unique natural laboratory due to its peculiar hydrology and physical features including temperature, salinity and water circulation. Moreover the Red Sea is
subjected to hydrocarbon pollution by both anthropogenic and natural sources that select hydrocarbon degrading prokaryotes. Due to its unique features the Red Sea has the potential to host uncharacterized novel microorganisms with hydrocarbondegrading pathways.
The focus of this thesis is on the characterization at the metagenomic level of the water column of the Red Sea and on the isolation and characterization of novel hydrocarbon-degrading species and genomes adapted to the unique environmental characteristics of the basin. The presence of metabolic genes responsible of both linear and aromatic hydrocarbon degradation has been evaluated from a metagenomic survey and a meta-analysis of already available datasets. In parallel, water column-based microcosms have been established with crude oil as the sole carbon source, with aim to isolate potential novel bacterial species and provide new genome-based insights on the hydrocarbon degradation potential available in the Red Sea.
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Matsumoto, M. "Aspects of the interaction between the marine bacterium Alcanivorax DG881 and the toxic dinoflagellate Gymnodinium catenatum." Thesis, 2011. https://eprints.utas.edu.au/12489/2/Matsumoto.pdf.
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The presence of a bacterial community is vital to the germination and growth of the toxic dinoflagellate Gymnodinium catenatum. Previous research has shown that the bacterium Alcanivorax DG881 is an important stimulatory member of the dinoflagellate-associated bacterial community, however the nature of the interaction between the two organisms, and the substances and mechanism involved in growth stimulation are unknown. This thesis uses a uni-bacterial G. catenatum experimental culture model to investigate elements of the interaction between the marine bacterium Alcanivorax DG881 with the dinoflagellate G. catenatum.
In the first experiment, three treatments were used to determine whether the G. catenatum growth stimulating substances produced by Alcanivorax DG881 were extracellular or intracellular substances, and whether these substances need to be continuously provided to G. catenatum to support growth. Addition of extracellular filtrates from cultures of G. catenatum and it’s associated bacteria showed increased growth stimulating activity in resting cyst germination experiments compared to treatments containing intracellular substances from Alcanivorax DG881 in absence of live Alcanivorax DG881 cells. Repeated addition of extracellular filtrates sustained G. catenatum growth after germination for a significantly longer period and to higher cell concentrations than a single addition of extracellular filtrate. These results indicated that the G. catenatum did not obtain growth stimulating substances by ingesting bacteria but requires one or more extracellular dissolved products produced by Alcanivorax DG881. The patterns of growth suggest that the products were either labile or utilised by the dinoflagellate during growth.
It has been proposed that dinoflagellate-associated Alcanivorax DG881 benefits from the utilization of dissolved organic carbon (DOC) exuded from the dinoflagellate cell. To examine this idea, the single carbon utilization profile of Alcanivorax DG881 was compared with the closely related but no-stimulatory strain Alcanivorax borkumensis SK2 using the BIOLOG GN2 plate assay system. Alcanivorax DG881 was able to use a much wider range of carbon compounds for growth than Alcanivorax borkumensis SK2, particular a wider range of amino acids, known as an important component of the DOC exuded from algal cells. The data here suggest that Alcanivorax DG881 is relatively better adapted to a life associated with algal cells than Alcanivorax borkumensis SK2. Detection and sequence characterization of putative saxitoxin synthesis gene homologues was attempted. Degenerate PCR primers designed from sequence of three putative saxitoxin biosynthesis (Sxt) genes from cyanobacteria was used to screen G. catenatum total DNA extracts. PCR products of expected length were obtained for three Sxt genes and two products were sequenced and compared to the putative cyanobacterial homolog and other published DNA sequences available on Genbank. The putative G. catenatum SxtN gene sequence showed highest similarity with sulfurtransferase of bacteria Francisella philomiragia subsp. Philomiragia (84% similarity), and with a hypothetical protein the Arabidopsis thaliana (81% similarity). The putative G. catenatum SxtU gene sequence showed low similarity with a hypothetical protein of Peptostreptococcus micros (46% similarity) and hypothetical proteins from the fungi Aspergillus oryzae (46% similarity). Phylogenetic comparisons of the partial sequences of both candidate genes suggested that they were of bacterial rather than dinoflagellate origin, and bacteria associated with G. catenatum not involved in saxitoxin synthesis directly or indirectly.
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Delgadillo, Ordoñez Nathalia Catalina. "INFLUENCE OF HYDROSTATIC PRESSURE AND TEMPERATURE ON THE METABOLIC ACTIVITY OF Alcanivorax marisrubri sp. nov. ISOLATED FROM THE RED SEA." Thesis, 2020. http://hdl.handle.net/10754/662609.
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Hydrostatic pressure (HP) and low temperature are among the main parameters that affect the microbial activity in the deep sea. Especially in the event of an oil spill, the natural microbial degradation of hydrocarbons in the harsh conditions of the deep sea can be significantly impaired. In the Red Sea, the temperature in the deep (22°C) is much higher than in other oceans and may favor hydrocarbon degradation. Bacteria of the genus Alcanivorax, which are prominent and ubiquitous alkane degrading bacteria, have been extensively studied because of their high abundance in oil-contaminated shallow water, but have been shown to be absent in the deep sea because of their piezo-sensitivity. In the present thesis, the novel species Alcanivorax marisrubri isolated at 1000 m from the Southern Red Sea has been evaluated for its piezo-adaptation under different combinations of temperature, and HP. A. marisrubri showed a piezotolerance different from other Alcanivorax species. Furthermore, a positive compensation of growth inhibition was observed when the cells were exposed to mild HP (10 MPa) in combination with a relatively high temperature of 38°C. While growth was inhibited at lower temperatures (20 and 26°C) under mild-HP (5 and 10 MPa), the metabolic activity was triggered, possibly in response to cellular stress. This study showed that the growth and metabolic activity of A. marisrubri under HP depend on temperature, which exerts a positive compensation effect and may extend the growth of this bacterium to the depths of the Red Sea.
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Sabirova, Julia [Verfasser]. "Functional genome analysis of Alcanivorax borkumensis strain SK2 : alkane metabolism, environmental adaptations and biotechnological potential / von Julia Sabirova." 2006. http://d-nb.info/981501915/34.
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Freitas, Carlos Jorge Batista. "Utilização de grânulos de cortiça para estimular a biorremediação aeróbia de hidrocarbonetos." Master's thesis, 2017. http://hdl.handle.net/1822/54662.
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Dissertação de mestrado integrado em Engenharia Biológica (área de especialização em Tecnologia Ambiental)Os derrames de petróleo constituem um dos desastres ambientais mais graves provocados pelo Homem. Uma das estratégias mais utilizadas para remediação de locais contaminados com petróleo é a contenção e remoção dos hidrocarbonetos por materiais sorventes. Entre estes, a cortiça tem sido aplicada com sucesso na remediação de derrames de óleos. Pelas suas características físico-químicas, a cortiça pode constituir um bom suporte para o crescimento de bactérias hidrocarbonoclásticas, ou conter substâncias que estimulem o seu crescimento, facilitando assim a ocorrência de biorremediação in situ. Este trabalho pretende avaliar a degradação aeróbia de hidrocarbonetos sorvidos em partículas regrânuladas de cortiça por bactérias hidrocarbonoclásticas, bem como estudar o efeito da presença da cortiça no crescimento deste tipo de bactérias. Foram realizados ensaios com duas culturas bacterianas (Alcanivorax borkumensis SK2 e Rhodoccocus opacus B4) e partículas de cortiça contaminados com uma mistura de alcanos (C14, C16, C20 e C24). Foram também efetuados ensaios controlo, em que as culturas bacterianas foram crescidas (i) com alcanos mas sem partículas de cortiça, e (ii) com cortiça mas sem adição da mistura de alcanos. O crescimento bacteriano foi avaliado ao longo do tempo, através da medição do azoto orgânico no material biológico em suspensão, e a degradação dos hidrocarbonetos foi monitorizada por cromatografia gasosa, após extração líquido-líquido. As partículas de cortiça tiveram um efeito estimulador direto sobre o crescimento de Alcanivorax borkumensis SK2 em alcanos, tendo-se obtido valores de azoto orgânico 1,4 vezes superiores na presença de cortiça, relativamente aos ensaios realizados sem cortiça, após 452 horas de incubação. Relativamente à degradação da mistura de alcanos, esta foi superior nos ensaios desenvolvidos na presença do que na ausência de cortiça, isto é, 72±2 % e 47±2 %, respetivamente. Estes resultados mostram o potencial desta estratégia para estimular a biorremediação in situ. Quanto às culturas de Rhodococcus opacus B4, a cortiça não teve um efeito estimulador sobre o crescimento. Relativamente à degradação da mistura de alcanos, após 283 horas de incubação esta atingiu 96±1 % e 89±3 % na presença e na ausência de cortiça, respetivamente.
Oil spills are one of the worst environmental disasters caused by anthropogenic activities. Containment of the oil spills and removal of hydrocarbons using sorbent materials is one of the most used strategies for remediation of the contaminated sites. Cork has been applied with success as biosorbent in the remediation of oil spills. Due to its physical-chemical characteristics, cork may be a good support for the growth of hydrocarbonoclastic bacteria, or may have substances which stimulate their growth, promoting in situ bioremediation. This work intends to evaluate the aerobic degradation of hydrocarbons sorbed in regranulated cork particles by hydrocarbonoclastic bacteria, as well as to study the effect of cork in the growth of these bacteria. Assays were made with two bacterial cultures (Alcanivorax borkumensis SK2 and Rhodoccocus opacus B4) and cork particles contaminated with a mixture of alkanes (C14, C16, C20 e C24). Control assays were also prepared, in which the bacterial cultures were grown (i) with alkanes but not with cork, and (ii) with cork without addition of the mixture of alkanes. The bacterial growth was evaluated along the time by measuring the organic nitrogen content in the biological suspended material, and the degradation of the hydrocarbons was monitored by gas chromatography after liquid-liquid extraction. Cork particles had a direct stimulator effect on the growth of Alcanivorax borkumensis SK2 on alkanes, as shown by the 1,4 times higher values of organic nitrogen measured in the assays with cork, relatively to the assays without cork, after 452 hours of incubation. Alkanes degradation was higher in the presence than in the absence of cork, i.e. 72±2 % and 47±2 %, respectively. These results show the potential of this strategy for stimulating in situ bioremediation. Concerning Rhodococcus opacus B4, cork did not stimulate the growth of this bacterium. Degradation of the mixture of alkanes by these cultures, after 283 hours of incubation, reached 96±1 % and 89±3 % in the presence and in the absence of cork, respectively.
Fundação para a Ciência e a Tecnologia (FCT), e pelo Fundo Europeu de Desenvolvimento Regional (FEDER), no âmbito dos projetos PTDC/AAG-TEC/3500/2014 (POCI-01-0145-FEDER-016575), UID/BIO/04469/2013 (POCI-01-0145-FEDER-006684), RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) e BioTecNorte operation (NORTE-01- 0145-FEDER-000004). Ana Cavaleiro agradece o apoio financeiro do European Research Council (ERC) no âmbito do projeto Novel Anaerobes (323009).
Book chapters on the topic "Alcanivorax"
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Cappello, S., and M. M. Yakimov. "Alcanivorax." In Handbook of Hydrocarbon and Lipid Microbiology, 1737–48. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-77587-4_123.
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Martins dos Santos, V., J. Sabirova, K. N. Timmis, M. M. Yakimov, and P. N. Golyshin. "Alcanivorax borkumensis." In Handbook of Hydrocarbon and Lipid Microbiology, 1265–88. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-77587-4_89.
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Shaojun, Zhang, Wang Mingyu, Liu Bingbing, Pang Shouwen, and Zhang Chengda. "ANNs Combined with Genetic Algorithm Optimization for Symbiotic Medium of Two Oil-Degrading Bacteria Cycloclasticus Sp. and Alcanivorax Sp." In Lecture Notes in Electrical Engineering, 1389–97. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-3648-5_179.
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Singh, Arvind K., Angela Sherry, Neil D. Gray, Martin D. Jones, Wilfred F. M. Röling, and Ian M. Head. "How Specific Microbial Communities Benefit the Oil Industry: Dynamics of Alcanivorax spp. in Oil-Contaminated Intertidal Beach Sediments Undergoing Bioremediation." In Applied Microbiology and Molecular Biology in Oilfield Systems, 199–209. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9252-6_24.
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WANG, WEE FUEN, and HAI MENG TAN. "ISOLATION, IDENTIFICATION AND MOLECULAR STUDIES OF ALCANIVORAX SP. LE4, A HYDROCARBON-DEGRADING MARINE BACTERIUM ISOLATED IN SINGAPORE." In Microbial Diversity in Asia, 177–97. WORLD SCIENTIFIC, 2001. http://dx.doi.org/10.1142/9789812811820_0010.
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