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Journal articles on the topic 'Alcanivorax'

Author: Grafiati
Published: 4 June 2021
Last updated: 20 February 2023

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1

Liao, Xianzhi, Qiliang Lai, Junpeng Yang, Chunming Dong, Dengfeng Li, and Zongze Shao. "Alcanivorax sediminis sp. nov., isolated from deep-sea sediment of the Pacific Ocean." International Journal of Systematic and Evolutionary Microbiology 70, no. 7 (July 1, 2020): 4280–84. http://dx.doi.org/10.1099/ijsem.0.004285.

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A taxonomic study was carried out on strain PA15-N-34T, which was isolated from deep-sea sediment of Pacific Ocean. The bacterium was Gram-stain-positive, oxidase- and catalase-positive and rod-shaped. Growth was observed at salinity of 0–15.0% NaCl and at temperatures of 10–45 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PA15-N-34T belonged to the genus Alcanivorax , with the highest sequence similarity to Alcanivorax profundi MTEO17T (97.7 %), followed by Alcanivorax nanhaiticus 19 m-6T (97.3 %) and 12 other species of the genus Alcanivorax (93.4 %–97.0 %). The average nucleotide identity and DNA–DNA hybridization values between strain PA15-N-34T and type strains of the genus Alcanivorax were 71.46–81.78% and 18.7–25.2 %, respectively. The principal fatty acids (>10 %) were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c; 31.2 %), C16 : 0 (25.0 %) and summed feature 3 (14.6 %). The DNA G+C content was 57.15 mol%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, four unidentified aminolipids and three unidentified lipids. The novel strain can be differentiated from its closest type strain by a negative test for urease and the presence of diphosphatidylglycerol and aminolipid. The combined genotypic and phenotypic data show that strain PA15-N-34T represents a novel species within the genus Alcanivorax , for which the name Alcanivorax sediminis sp. nov. is proposed, with the type strain PA15-N-34T (=MCCC 1A14738T=KCTC 72163T).
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2

Liu, Chenli, and Zongze Shao. "Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment." International Journal of Systematic and Evolutionary Microbiology 55, no. 3 (May 1, 2005): 1181–86. http://dx.doi.org/10.1099/ijs.0.63443-0.

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Two bacterial strains, B-5T and NO1A, were isolated from the surface water of the Bohai Sea and deep-sea sediment of the east Pacific Ocean, respectively. Both strains were halophilic, aerobic, Gram-negative, non-spore-forming, catalase- and oxidase-positive motile rods. They grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. On the basis of 16S rRNA gene sequence similarity, strains B-5T and NO1A were shown to belong to the γ-Proteobacteria. Highest similarity values were found with Alcanivorax venustensis (95·2 %), Alcanivorax jadensis (94·6 %) and Alcanivorax borkumensis (94·1 %). Principal fatty acids of both strains were C16 : 0, C16 : 1 ω7c and C18 : 1 ω7c. The chemotaxonomically characteristic fatty acid C19 : 0 cyclo ω8c was also detected. On the basis of the above, together with results of physiological and biochemical tests, DNA–DNA hybridization, comparisons of 16S–23S internal transcribed spacer sequences and comparisons of the partial deduced amino acid sequence of alkane hydroxylase, both strains were affiliated to the genus Alcanivorax but were differentiated from recognized Alcanivorax species. Therefore, a novel species, Alcanivorax dieselolei sp. nov., represented by strains B-5T and NO1A is proposed, with the type strain B-5T (=DSM 16502T=CGMCC 1.3690T).
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3

Kyoung Kwon, Kae, Ji Hye Oh, Sung-Hyun Yang, Hyun-Seok Seo, and Jung-Hyun Lee. "Alcanivorax gelatiniphagus sp. nov., a marine bacterium isolated from tidal flat sediments enriched with crude oil." International Journal of Systematic and Evolutionary Microbiology 65, Pt_7 (July 1, 2015): 2204–8. http://dx.doi.org/10.1099/ijs.0.000244.

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A Gram-reaction-negative, rod-shaped marine bacterium, designated MEBiC08158T, was isolated from sediments collected from Taean County, Korea, near the Hebei Spirit tanker oil spill accident. 16S rRNA gene sequence analysis revealed that strain MEBiC08158T was closely related to Alcanivorax marinus R8-12T (99.5 % similarity) but was distinguishable from other members of the genus Alcanivorax (93.7–97.1 %). The DNA–DNA hybridization value between strain MEBiC08158T and A. marinus R8-12T was 58.4 %. Growth of strain MEBiC08158T was observed at 15–43 °C (optimum 37–40 °C), at pH 6.0–9.5 (optimum pH 7.0–8.0) and with 0.5–16 % NaCl (optimum 1.5–3.0 %). The dominant fatty acids were C16 : 0, C19 : 0 cyclo ω8c, C12 : 0, C18 : 1ω7c, C12 : 0 3-OH and summed feature 3 (comprising C15 : 0 2-OH and/or C16 : 1ω7c). Several phenotypic characteristics differentiate strain MEBiC08158T from phylogenetically close members of the genus Alcanivorax. Therefore, strain MEBiC08158T should be classified as representing a novel species of the genus Alcanivorax, for which the name Alcanivorax gelatiniphagus sp. nov. is proposed. The type strain is MEBiC08158T ( = KCCM 42990T = JCM 18425T).
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4

Lai, Qiliang, Jianning Wang, Li Gu, Tianling Zheng, and Zongze Shao. "Alcanivorax marinus sp. nov., isolated from deep-sea water." International Journal of Systematic and Evolutionary Microbiology 63, Pt_12 (December 1, 2013): 4428–32. http://dx.doi.org/10.1099/ijs.0.049957-0.

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A taxonomic study was carried out on strain R8-12T, which was isolated from deep-sea water of the Indian Ocean during the screening of oil-degrading bacteria. The isolate was Gram-stain-negative, oxidase and catalase-positive. Growth was observed at salinities from 0.5 to 15 % (optimum 3 %), at pH from 6–10 (optimum 7–8) and at temperatures from 10 to 42 °C (optimum 28 °C). On the basis of 16S rRNA gene sequence similarity, strain R8-12T was shown to belong to the genus Alcanivorax and to be related to Alcanivorax venustensis DSM 13974T (97.2 %), A. dieselolei B-5T (95.0 %), A. balearicus MACL04T (94.6 %), A. hongdengensis A-11-3T (94.3 %), A. jadensis T9T (93.8 %), A. borkumensis SK2T (93.7 %) and A. pacificus W11-5T (93.7 %). The gyrB sequence similarities between R8-12T and other species of the genus Alcanivorax ranged from 77.9 % to 86.9 %. The major fatty acids were C16 : 0 (31.8 %), C18 : 1ω7c (20.3 %), C19 : 0ω8c cyclo (15.8 %) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) (8.9 %). The polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two aminolipids (AL1–AL2) and two phospholipids (PL1–PL2). Three alkane hydroxylase (alkB) genes were identified in the genome. The G+C content of the chromosomal DNA was 66.1 mol%. DNA–DNA hybridization showed that strain R8-12T and A. venustensis DSM 13974T had a DNA–DNA relatedness of 63±3 %. According to its phenotypic features and fatty acid composition as well as the 16S rRNA and gyrB gene sequences, the novel strain represents a member of the genus Alcanivorax , but could be easily distinguished from all other known species of the genus Alcanivorax described to date. The name Alcanivorax marinus sp. nov. is proposed, with the type strain R8-12T ( = MCCC 1A00382T = LMG 24621T = CCTCC AB 208234T).
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5

Lai, Qiliang, Liping Wang, Yuhui Liu, Yuanyuan Fu, Huanzi Zhong, Baojiang Wang, Liang Chen, Jianning Wang, Fengqin Sun, and Zongze Shao. "Alcanivorax pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium." International Journal of Systematic and Evolutionary Microbiology 61, no. 6 (June 1, 2011): 1370–74. http://dx.doi.org/10.1099/ijs.0.022368-0.

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A taxonomic study was carried out on a novel bacterial strain, designated W11-5T, which was isolated from a pyrene-degrading consortium enriched from deep-sea sediment of the Pacific Ocean. The isolate was Gram-reaction-negative and oxidase- and catalase-positive. Growth was observed in 0.5–12 % (w/v) NaCl and at 10–42 °C. On the basis of 16S rRNA gene sequence analysis, strain W11-5T was shown to belong to the genus Alcanivorax with a close relation to A. dieselolei B-5T (93.9 % 16S rRNA sequence similarity), A. balearicus MACL04T (93.1 %), A. hongdengensis A-11-3T (93.1 %), A. borkumensis SK2T (93.0 %), A. venustensis ISO4T (93.0 %) and A. jadensis T9T (92.9 %). Similarities between the gyrB gene sequences of W11-5T and other species of the genus Alcanivorax were between 76.8 and 80.8 %. The principal fatty acids were C12 : 0 3-OH (8.0 %), C16 : 0 (29.1 %) and C18 : 1ω7c (27.4 %). The G+C content of the chromosomal DNA was 60.8 mol%. Based on its morphology, physiology and fatty acid composition as well as the results of 16S rRNA and gyrB gene sequence analyses, strain W11-5T ( = MCCC 1A00474T = CCTCC AB 208236T = LMG 25514T) represents a novel species of the genus Alcanivorax, for which the name Alcanivorax pacificus sp. nov. is proposed.
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6

Rahul, K., Ch Sasikala, L. Tushar, R. Debadrita, and Ch V. Ramana. "Alcanivorax xenomutans sp. nov., a hydrocarbonoclastic bacterium isolated from a shrimp cultivation pond." International Journal of Systematic and Evolutionary Microbiology 64, Pt_10 (October 1, 2014): 3553–58. http://dx.doi.org/10.1099/ijs.0.061168-0.

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Two bacterial strains (JC109T and JC261) were isolated from a sediment sample collected from a shrimp cultivation pond in Tamil Nadu (India). Cells were Gram-stain-negative, motile rods. Both strains were positive for catalase and oxidase, hydrolysed Tween 80, and grew chemo-organoheterotrophically with an optimal pH of 6 (range pH 4–9) and at 30 °C (range 25–40 °C). Based on 16S rRNA gene sequence analysis, strains JC109T and JC261 were identified as belonging to the genus Alcanivorax with Alcanivorax dieselolei B-5T (sequence similarity values of 99.3 and 99.7 %, respectively) and Alcanivorax balearicus MACL04T (sequence similarity values of 98.8 and 99.2 %, respectively) as their closest phylogenetic neighbours. The 16S rRNA gene sequence similarity between strains JC109T and JC261 was 99.6 %. The level of DNA–DNA relatedness between the two strains was 88 %. Strain JC109T showed 31±1 and 26±2 % DNA–DNA relatedness with A. dieselolei DSM 16502T and A. balearicus DSM 23776T, respectively. The DNA G+C content of strains JC109T and JC261 was 54.5 and 53.4 mol%, respectively. Polar lipids of strain JC109T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids. The major fatty acids were C10 : 0, C12 : 0, C16 : 0, C12 : 0 3-OH, C16 : 1ω7c, C18 : 1ω7c and C19 : 0 cyclo ω8c. Both strains could utilize diesel oil and a variety of xenobiotics as carbon and energy sources. The results of physiological, biochemical, chemotaxonomic and molecular analyses allowed the clear differentiation of strains JC109T and JC261 from all other members of the genus Alcanivorax . Strains JC109T and JC261 are thus considered to represent a novel species, for which the name Alcanivorax xenomutans sp. nov. is proposed. The type strain is JC109T ( = KCTC 23751T = NBRC 108843T).
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7

Manilla-Pérez, Efraín, Christina Reers, Meike Baumgart, Stephan Hetzler, Rudolf Reichelt, Ursula Malkus, Rainer Kalscheuer, Marc Wältermann, and Alexander Steinbüchel. "Analysis of Lipid Export in Hydrocarbonoclastic Bacteria of the Genus Alcanivorax: Identification of Lipid Export-Negative Mutants of Alcanivorax borkumensis SK2 and Alcanivorax jadensis T9." Journal of Bacteriology 192, no. 3 (November 20, 2009): 643–56. http://dx.doi.org/10.1128/jb.00700-09.

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ABSTRACT Triacylglycerols (TAGs), wax esters (WEs), and polyhydroxyalkanoates (PHAs) are the major hydrophobic compounds synthesized in bacteria and deposited as cytoplasmic inclusion bodies when cells are cultivated under imbalanced growth conditions. The intracellular occurrence of these compounds causes high costs for downstream processing. Alcanivorax species are able to produce extracellular lipids when the cells are cultivated on hexadecane or pyruvate as the sole carbon source. In this study, we developed a screening procedure to isolate lipid export-negative transposon-induced mutants of bacteria of the genus Alcanivorax for identification of genes required for lipid export by employing the dyes Nile red and Solvent Blue 38. Three transposon-induced mutants of A. jadensis and seven of A. borkumensis impaired in lipid secretion were isolated. All isolated mutants were still capable of synthesizing and accumulating these lipids intracellularly and exhibited no growth defect. In the A. jadensis mutants, the transposon insertions were mapped in genes annotated as encoding a putative DNA repair system specific for alkylated DNA (Aj17), a magnesium transporter (Aj7), and a transposase (Aj5). In the A. borkumensis mutants, the insertions were mapped in genes encoding different proteins involved in various transport processes, like genes encoding (i) a heavy metal resistance (CZCA2) in mutant ABO_6/39, (ii) a multidrug efflux (MATE efflux) protein in mutant ABO_25/21, (iii) an alginate lyase (AlgL) in mutants ABO_10/30 and ABO_19/48, (iv) a sodium-dicarboxylate symporter family protein (GltP) in mutant ABO_27/29, (v) an alginate transporter (AlgE) in mutant ABO_26/1, or (vi) a two-component system protein in mutant ABO_27/56. Site-directed MATE, algE, and algL gene disruption mutants, which were constructed in addition, were also unable to export neutral lipids and confirmed the phenotype of the transposon-induced mutants. The putative localization of the different gene products and their possible roles in lipid excretion are discussed. Beside this, the composition of the intra- and extracellular lipids in the wild types and mutants were analyzed in detail.
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8

Puspitasari, Ita, Agus Trianto, and Jusup Supriyanto. "Eksplorasi Bakteri Pendegradasi Minyak dari Perairan Pelabuhan Tanjung Mas, Semarang." Journal of Marine Research 9, no. 3 (July 16, 2020): 281–88. http://dx.doi.org/10.14710/jmr.v9i3.27606.

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ABSTRAK: Tanjung Mas merupakan salah satu kawasan pelabuhan di Jawa Tengah yang terus mengalami peningkatan jumlah kapal setiap tahunnya. Hal ini berimbas pada semakin meningkatnya tumpahan minyak solar yang masuk ke perairan. Upaya yang bisa dilakukan untuk mengurangi jumlah tumpahan minyak solar di Pelabuhan Tanjung Mas yaitu dengan melakukan penelitian bioremediasi menggunakan bakteri indigenouse. Tujuan penelitian ini dilakukan yaitu mendapatkan isolat bakteri air laut asal Pelabuhan Tanjung Mas, Semarang yang mampu mendegradasi minyak solar dan mengetahui kemampuan masing-masing isolat untuk mendegradasi minyak solar. Penelitian ini dilaksanakan pada bulan Juli sampai Oktober 2019 dengan pengambilan sample air laut menggunakan metode purpossive sampling, metode isolasi menggunakan pour plate, metode uji minyak secara gravimetri dan pertumbuhan bakteri menggunakan Standart Plate Count (SPC). Hasil isolasi yaitu didapatkan 2 isolat unggul yaitu bakteri Alcanivorax nanhaiticus dan Halomonas meridiana. Bakteri Alcanivorax nanhaiticus mampu mendegradasi 54% minyak solar dan Halomonas meridiana mampu mendegradasi 72% minyak solar. Kedua bakteri merupakan bakteri hidrokarbonoklastik atau bakteri yang memiliki kemampuan mendegradasi minyak solar (hidrokarbon) karena mengandung enzim monooksigenase. Dari penelitian ini dapat disimpulkan bahwa di Pelabuhan Tanjung Mas terdapat bakteri yang mampu mendegradasi minyak solar yaitu Alcanivorax nanhaiticus dan Halomonas meridiana. Kedua isolat mampu mendegradasi lebih dari 50% minyak solar yang diujikan. ABSTRACT: Tanjung Mas is one of the port areas in Central Java that continues to experience an increase in the number of ships each year. This has an impact on the increasing amount of diesel fuel spills that enter the waters. Efforts that can be made to reduce the number of diesel fuel spills in the Port of Tanjung Mas is to conduct bioremediation research using indigenous bacteria. The purpose of this study was to obtain seawater bacterial isolates from the Port of Tanjung Mas, Semarang that can degrade diesel fuel and determine the ability of each isolate to degrade diesel fuel. This research was conducted in July to October 2019 by taking seawater samples using a purposive sampling method, isolation method using pour plates, gravimetric fuel test methods and bacterial growth using Standard Plate Count (SPC). The results of the isolation were obtained 2 superior isolates namely Alcanivorax nanhaiticus and Halomonas meridiana. Alcanivorax nanhaiticus can degrade 54% of diesel fuel and Halomonas meridiana can degrade 72% of diesel fuel. Both bacteria are hydrocarbonoclastic bacteria or bacteria that can degrade diesel oil (hydrocarbons) because they contain the enzyme monooxsigenase. From this study, it can be concluded that at Tanjung Mas Port there are bacteria that can degrade diesel fuel, namely Alcanivorax nanhaiticus and Halomonas meridiana. Both isolates were able to degrade more than 50% of the tested diesel fuel.
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Teramoto, Maki, Masahito Suzuki, Fumiyoshi Okazaki, Ariani Hatmanti, and Shigeaki Harayama. "Oceanobacter-related bacteria are important for the degradation of petroleum aliphatic hydrocarbons in the tropical marine environment." Microbiology 155, no. 10 (October 1, 2009): 3362–70. http://dx.doi.org/10.1099/mic.0.030411-0.

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Petroleum-hydrocarbon-degrading bacteria were obtained after enrichment on crude oil (as a ‘chocolate mousse’) in a continuous supply of Indonesian seawater amended with nitrogen, phosphorus and iron nutrients. They were related to Alcanivorax and Marinobacter strains, which are ubiquitous petroleum-hydrocarbon-degrading bacteria in marine environments, and to Oceanobacter kriegii (96.4–96.5 % similarities in almost full-length 16S rRNA gene sequences). The Oceanobacter-related bacteria showed high n-alkane-degrading activity, comparable to that of Alcanivorax borkumensis strain SK2. On the other hand, Alcanivorax strains exhibited high activity for branched-alkane degradation and thus could be key bacteria for branched-alkane biodegradation in tropical seas. Oceanobacter-related bacteria became most dominant in microcosms that simulated a crude oil spill event with Indonesian seawater. The dominance was observed in microcosms that were unamended or amended with fertilizer, suggesting that the Oceanobacter-related strains could become dominant in the natural tropical marine environment after an accidental oil spill, and would continue to dominate in the environment after biostimulation. These results suggest that Oceanobacter-related bacteria could be major degraders of petroleum n-alkanes spilt in the tropical sea.
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Cappello, Simone, Ilaria Corsi, Sabrina Patania, Elisa Bergami, Maurizio Azzaro, Monique Mancuso, Maria Genovese, Alessia Lunetta, and Gabriella Caruso. "Characterization of Five Psychrotolerant Alcanivorax spp. Strains Isolated from Antarctica." Microorganisms 11, no. 1 (December 24, 2022): 58. http://dx.doi.org/10.3390/microorganisms11010058.

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Five psychrotolerant Alcanivorax spp. strains were isolated from Antarctic coastal waters. Strains were screened for molecular and physiological properties and analyzed regarding their growth capacity. Partial 16S rDNA, alk-B1, and P450 gene sequencing was performed. Biolog EcoPlates and the API 20E test were used to evaluate metabolic and biochemical profiles. Bacterial growth in sodium acetate was determined at 4, 15, 20, and 25 °C to evaluate the optimal temperature. Furthermore, the ability of each strain to grow in a hydrocarbon mixture at 4 and 25 °C was assayed. Biosurfactant production tests (drop-collapse and oil spreading) and emulsification activity tests (E24) were also performed. Concerning results of partial gene sequencing (16S rDNA, alk-B1, and P450), a high similarity of the isolates with the same genes isolated from other Alcanivorax spp. strains was observed. The metabolic profiles obtained by Biolog assays showed no significant differences in the isolates compared to the Alcanivorax borkumensis wild type. The results of biodegradative tests showed their capability to grow at different temperatures. All strains showed biosurfactant production and emulsification activity. Our findings underline the importance to proceed in the isolation and characterization of Antarctic hydrocarbon-degrading bacterial strains since their biotechnological and environmental applications could be useful even for pollution remediation in polar areas.
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11

Song, Lei, Hongcan Liu, Shichun Cai, Ying Huang, Xin Dai, and Yuguang Zhou. "Alcanivorax indicus sp. nov., isolated from seawater." International Journal of Systematic and Evolutionary Microbiology 68, no. 12 (December 1, 2018): 3785–89. http://dx.doi.org/10.1099/ijsem.0.003058.

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12

Fernández-Martínez, Javier, María J. Pujalte, Jesús García-Martínez, Manuel Mata, Esperanza Garay, and Francisco Rodríguez-Valera. "Description of Alcanivorax venustensis sp. nov. and reclassification of Fundibacter jadensis DSM 12178T (Bruns and Berthe-Corti 1999) as Alcanivorax jadensis comb. nov., members of the emended genus Alcanivorax." International Journal of Systematic and Evolutionary Microbiology 53, no. 1 (January 1, 2003): 331–38. http://dx.doi.org/10.1099/ijs.0.01923-0.

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13

Alfiansah, Yustian Rovi, Mindi Adindasari, Mentari Argarini, Yeti Darmayati, and Ruyitno. "ISOLATION AND DISTRIBUTION OF CRUDE OIL AND POLYCYCLIC AROMATIC HYDROCARBON-DEGRADING BACTERIA FROM POLLUTED HARBOURS IN NORTH JAKARTA." Marine Research in Indonesia 39, no. 2 (November 20, 2015): 79–85. http://dx.doi.org/10.14203/mri.v39i2.49.

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Several harbours in North Jakarta have been polluted by spills of oil and their derivates. We suggest that diverse species of crude oil and polycyclic aromatic hydrocarbon-degrading bacteria  inhabit these harbours. An experiment was undertaken in 2007 to isolate crude oil and polycyclic aromatic hydrocarbon (PAH)-degrading bacteria from oil-polluted harbours, such as Muara Baru, Sunda Kelapa and Tanjung Priok. Sea water and sediment samples were collected twice, in March and April. Crude oil and PAH-degrading bacteria were isolated from enrichment culture of samples in an enrichment medium (SWP), using ONR7a medium with the addition of 5 types of PAH gases or Arabian Light Crude Oil 210 (ALCO 210) onto medium. This study reported that fluoranthene and crude oil-degrading bacteria were the major bacteria isolated from the three polluted harbours. In total, 109 isolates have been collected which can degrade crude oil (29% of total isolates), fluoranthene (33%), fluorene (20%), pyrene (7%), dibenzothiopene (6%), and phenantrene (5 %). Among these isolates, 5 isolates have the capability to degrade 5 types of PAH and ALCO 210. They were Alcanivorax sp. B-1084, Pseudomonas sp. D5-38b, Alcanivorax sp. TE-9, Bacillus sp. L41, Alcanivorax dieselolei strain B-5 clone 1. Culturable bacteria have been isolated mostly from the Sunda Kelapa samples, with fewer in those from Muara Baru and Tanjung Priok, respectively
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Rivas, Raúl, Paula García-Fraile, Alvaro Peix, Pedro F. Mateos, Eustoquio Martínez-Molina, and Encarna Velázquez. "Alcanivorax balearicus sp. nov., isolated from Lake Martel." International Journal of Systematic and Evolutionary Microbiology 57, no. 6 (June 1, 2007): 1331–35. http://dx.doi.org/10.1099/ijs.0.64912-0.

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A bacterial strain designated MACL04T was isolated from Lake Martel, a subterraneous saline lake in Mallorca (Spain). The complete 16S rRNA gene sequence of this strain showed nearly 100 % similarity to that of Alcanivorax dieselolei B-5T. Despite this high similarity, strain MACL04T showed phenotypic, chemotaxonomic and molecular differences with respect to A. dieselolei, indicating that strain MACL04T represents a separate species. Cells of strain MACL04T were motile by means of a single polar or subpolar flagellum and colonies formed on media containing 1 % (v/v) Tween 20 were opaque and mucoid, with blue–green iridescence. The generation time of strain MACL04T in this medium was approximately half that of A. dieselolei B-5T and strain MACL04T did not produce lipases after incubation for 5 days. Strain MACL04T did not require NaCl for growth and grew in the presence of up to 15 % (w/v) NaCl. The strain was able to use alkanes as a sole carbon source; however, glucose could also be used, albeit weakly, as a carbon source. Several amino acids and organic acids were used as carbon sources. Strain MACL04T produced acid in media containing pyruvate as the sole carbon source. The major fatty acids were C19 : 0 cyclo ω8c and C16 : 0. The fatty acid C16 : 1 ω8c, present in strain MACL04T, was not detected in the recognized Alcanivorax species. The sequences of the large and short 16S–23S intergenic spacer regions showed similarities of 97.2 and 98.8 % (ungapped) with respect to A. dieselolei B-5T. Partial sequences of gyrB and alkb genes showed 94.0 % similarity between strain MACL04T and A. dieselolei B-5T. The G+C content of strain MACL04T was 62.8 mol%. The data from this polyphasic study indicate that strain MACL04T represents a novel species of the genus Alcanivorax, for which the name Alcanivorax balearicus sp. nov. is proposed. The type strain is MACL04T (=LMG 22508T=CECT 5683T).
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Darmayati, Yeti, Harpasis S. Sanusi, Tri Prartono, Dwi Andreas Santosa, and Ruyitno Ruyitno. "The Efficacy of Bioaugmentation on Remediating Oil Contaminated Sandy Beach Using Mesocosm Approach (Efikasi Tehnik Bioaugmentasi dalam Memulihkan Pantai Berpasir Tercemar Minyak Menggunakan Pendekatan Mesokosm)." ILMU KELAUTAN: Indonesian Journal of Marine Sciences 20, no. 3 (September 2, 2015): 143. http://dx.doi.org/10.14710/ik.ijms.20.3.143-152.

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Bioremediation is basically consists of two approaches, biostimulation and bioaugmentation. The efficacy of bioaugmentation for combating oil pollution in field application is still argued. The purpose of study was to evaluate the efficacy of bioaugmentation and to compare the affectivity of single strain and consortium application in remediating oil polluted sandy beach. Experimental study in a field has been conducted with two (2) treatments and one (1) control in three different plots. The treatmens were introduction of a single strain (Alcanivorax sp TE-9) and a consortium (Alcanivorax sp. TE-9, Pseudomonas balearica st 101 and RCO/B/08-015) cultures into oil contaminated sediment. The experiment in mesocosm approach was taken place in Cilacap coast. Arabian light crude oil was used in the concentration of 100.000 mg.kg-1 sediment. Changes of oil concentration, bacterial density and pore water quality have been monitored periodically for 3 months. The result showed that oil degradation percentage and bacterial growth in both treatments were higher than in control. After 3 months, the percentage of oil degradation experiment in control, single strain and formulated consortium treatments were observed at 60.4%, 74.5% and 73.5%. It proves that bioaugmentation tehnique can enhance significantly oil biodegradation in sandy beach. The applications of bacteria in single or consortium culture give no different impact on their affectivity for bioremediation in Cilacap sandy beach. By data extrapolation it can be predicted that both of treatments able to reduce remediation time from 210 days into 135–137 days. Bioaugmentation can be proposed as a good solution for finalizing oil removing in Cilacap sandy beach when oil spilled occurred in this environment. Keywords: Bioremediation, bioaugmentation, oil, sandy beach, Alcanivorax, mesocosm, Cilacap Bioremediasi pada dasarnya terdiri dari dua pendekatan yaitu biostimulasi dan bioaugmentasi. Teknik bioaugmentasi dalam menanggulangi pencemaran minyak di lapangan masih diperdebatkan efektivitasnya. Penelitian ini bertujuan untuk mengevaluasi efikasi tehnik bioaugmentasi serta membandingkan efektivitas kultur tunggal dan konsorsium dalam memulihkan pantai berpasir tercemar minyak. Studi eksperimental di lapangan telah dilakukan dengan menggunakan dua perlakuan dan satu kontrol di tiga plot berbeda, Perlakuannya adalah penambahan bakteri kultur tunggal (Alcanivorax sp TE-9) dan bakteri konsorsium (Alcanivorax sp. TE-9, Pseudomonas balearica st 101 dan RCO/B/08-015) ke dalam sedimen yang tercemar minyak. Eksperimen dengan pendekatan mesokosm dilakukan di pantai Cilacap. Minyak mentah ringan Arabia dengan konsentrasi 100.000 mg.kg-1 sedimen digunakan sebagai bahan cemaran. Perubahan konsentrasi minyak, kepadatan bakteri dan parameter lingkungan diamati secara periodik selama 3 bulan percobaan. Hasil penelitian menunjukkan bahwa persentase degradasi minyak dan densitas bakteri di sedimen perlakuan lebih tinggi daripada kontrolnya. Setelah 3 bulan eksperimen, persentase degradasi minyak pada kontrol, perlakuan kultur tunggal dan konsorsium masing-masing teramati 60.4%, 74.5% and 73.5%. Hal ini membuktikan bahwa tehnik bioaugmentasi secara signifikan mampu meningkatkan biodegradasi minyak di pantai berpasir. Pemberian mikroba dalam bentuk kultur tunggal ataupun konsorsium mempunyai efektivitas yang tidak berbeda untuk proses bioremediasi di pantai berpasir Cilacap. Dengan ekstrapolasi data dapat diduga bahwa kedua perlakuan bioaugmentasi ini mampu mempercepat waktu pemulihan lingkungan dari 210 hari ke 135-137 hari. Bioaugmentasi dapat diusulkan sebagai solusi yang cukup baik untuk menghilangkan minyak pada tahap akhir pembersihan pantai berpasir Cilacap, jika terjadi tumpahan minyak di lingkungan ini. Kata kunci: single strain, consortium, bioaugmentation, oil, bioremediation, sandy beach, Alcanivorax, Cilacap
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Reva, Oleg N., Peter F. Hallin, Hanni Willenbrock, Thomas Sicheritz-Ponten, Burkhard Tümmler, and David W. Ussery. "Global features of the Alcanivorax borkumensis SK2 genome." Environmental Microbiology 10, no. 3 (March 2008): 614–25. http://dx.doi.org/10.1111/j.1462-2920.2007.01483.x.

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Teimoori, Afsaneh, Shahin Ahmadian, Armin Madadkar-Sobhani, and Bijan Bambai. "Rubredoxin reductase from Alcanivorax borkumensis: Expression and characterization." Biotechnology Progress 27, no. 5 (June 28, 2011): 1383–89. http://dx.doi.org/10.1002/btpr.653.

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18

Sutiknowati, Lies Indah. "BIOREMEDIATION STUDY: HYDROCARBON DEGRADING BACTERIA." Marine Research in Indonesia 32, no. 2 (May 12, 2018): 95–101. http://dx.doi.org/10.14203/mri.v32i2.442.

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Many microorganisms capable of degrading petroleum components have been isolated and few of them seem to be important for petroleum biodegradation in natural environments. To identify the bacteria that play a major role in degradation of petroleum polynuclear aromatic hydrocarbons (PAHs), bacteria were enriched from seawater by using Naphthalene, Phenanthrene, Trichlorodibenzofuran and Benzo[a]pyrene as a carbon and energy source. The result of study that members of the genus Alcanivorax and Thalassospira became predominant in the enrichment cultures. The strains isolated in this study could grow on crude oil and degraded PAH components of crude oil. The number of cells increased to 8.1x106 cells g-1 after 14 days in subculture. PAH degradation proceeded parallel with the growth of bacteria cells. This observation which has been conducted in Marine Biotechnology Institute, Kamaishi, Iwate-ken, Japan suggests that Alcanivorax and Thalassospira play an important role in the degradation of petroleum PAHs in marine environment.
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Lai, Qiliang, Zhongwen Zhou, Guizhen Li, Guangyu Li, and Zongze Shao. "Alcanivorax nanhaiticus sp. nov., isolated from deep sea sediment." International Journal of Systematic and Evolutionary Microbiology 66, no. 9 (September 1, 2016): 3651–55. http://dx.doi.org/10.1099/ijsem.0.001247.

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20

Lai, Qiliang, Weiwei Li, and Zongze Shao. "Complete Genome Sequence of Alcanivorax dieselolei Type Strain B5." Journal of Bacteriology 194, no. 23 (November 9, 2012): 6674. http://dx.doi.org/10.1128/jb.01813-12.

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ABSTRACTAlcanivorax dieseloleiB5Twas isolated from oil-contaminated surface water of the Bohai Sea of China and characterized by the efficient degradation of alkane (C5-C36). Here we report the complete genome of B5Tand genes associated with alkane degradation.
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21

Wei, Guangshan, Sujie Li, Sida Ye, Zining Wang, Kourosh Zarringhalam, Jianguo He, Wanpeng Wang, and Zongze Shao. "High-Resolution Small RNAs Landscape Provides Insights into Alkane Adaptation in the Marine Alkane-Degrader Alcanivorax dieselolei B-5." International Journal of Molecular Sciences 23, no. 24 (December 15, 2022): 15995. http://dx.doi.org/10.3390/ijms232415995.

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Alkanes are widespread in the ocean, and Alcanivorax is one of the most ubiquitous alkane-degrading bacteria in the marine ecosystem. Small RNAs (sRNAs) are usually at the heart of regulatory pathways, but sRNA-mediated alkane metabolic adaptability still remains largely unknown due to the difficulties of identification. Here, differential RNA sequencing (dRNA-seq) modified with a size selection (~50-nt to 500-nt) strategy was used to generate high-resolution sRNAs profiling in the model species Alcanivorax dieselolei B-5 under alkane (n-hexadecane) and non-alkane (acetate) conditions. As a result, we identified 549 sRNA candidates at single-nucleotide resolution of 5′-ends, 63.4% of which are with transcription start sites (TSSs), and 36.6% of which are with processing sites (PSSs) at the 5′-ends. These sRNAs originate from almost any location in the genome, regardless of intragenic (65.8%), antisense (20.6%) and intergenic (6.2%) regions, and RNase E may function in the maturation of sRNAs. Most sRNAs locally distribute across the 15 reference genomes of Alcanivorax, and only 7.5% of sRNAs are broadly conserved in this genus. Expression responses to the alkane of several core conserved sRNAs, including 6S RNA, M1 RNA and tmRNA, indicate that they may participate in alkane metabolisms and result in more actively global transcription, RNA processing and stresses mitigation. Two novel CsrA-related sRNAs are identified, which may be involved in the translational activation of alkane metabolism-related genes by sequestering the global repressor CsrA. The relationships of sRNAs with the characterized genes of alkane sensing (ompS), chemotaxis (mcp, cheR, cheW2), transporting (ompT1, ompT2, ompT3) and hydroxylation (alkB1, alkB2, almA) were created based on the genome-wide predicted sRNA–mRNA interactions. Overall, the sRNA landscape lays the ground for uncovering cryptic regulations in critical marine bacterium, among which both the core and species-specific sRNAs are implicated in the alkane adaptive metabolisms.
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Böttger, Sebastian, Silke Zechel-Gran, Philipp Streckbein, Michael Knitschke, Torsten Hain, Markus Weigel, Jan-Falco Wilbrand, Eugen Domann, Hans-Peter Howaldt, and Sameh Attia. "A New Type of Chronic Wound Infection after Wisdom Tooth Extraction: A Diagnostic Approach with 16S-rRNA Gene Analysis, Next-Generation Sequencing, and Bioinformatics." Pathogens 9, no. 10 (September 28, 2020): 798. http://dx.doi.org/10.3390/pathogens9100798.

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Delayed-onset infections are rare postoperative complications of lower third molar extractions. This article presents a case of a chronic combined hard and soft tissue infection after the extraction of a third molar, where the causative organisms could only be elucidated by molecular methods. Experimental 16S-rRNA gene analysis with next-generation sequencing and bioinformatics was used to identify the bacterial spectrum of the infection. 16S-rRNA gene analysis delivered the microbiome of the abscessing inflammation while standard culture and laboratory examinations were all sterile. The microbiome showed a mixed bacterial infection with a dominance of Delftia and Alcanivorax (spp.) besides other bacteria of the normal oral flora. Using 16S-rRNA-gene analysis, next-generation sequencing, and bioinformatics, a new type of chronic wound infection after wisdom tooth extraction was found. The property of Delftia and Alcanivorax (spp.) as water-affine environmental bacteria raises suspicion of infection from contaminated water from a dental unit. Thus, osteotomies of teeth should only be done with sterile cooling water. The 16S-rRNA gene analysis should become a part of the routine diagnostics in medical microbiology.
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Dutta, Tapan K., and Shigeaki Harayama. "Biodegradation ofn-Alkylcycloalkanes and n-Alkylbenzenes via New Pathways in Alcanivorax sp. Strain MBIC 4326." Applied and Environmental Microbiology 67, no. 4 (April 1, 2001): 1970–74. http://dx.doi.org/10.1128/aem.67.4.1970-1974.2001.

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ABSTRACT The degradation of long-chain n-alkylbenzenes andn-alkylcyclohexanes by Alcanivorax sp. strain MBIC 4326 was investigated. The alkyl side chain of these compounds was mainly processed by β-oxidation. In the degradation ofn-alkylcyclohexanes, cyclohexanecarboxylic acid was formed as an intermediate. This compound was further transformed to benzoic acid via 1-cyclohexene-1-carboxylic acid.
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Kadri, Tayssir, Sara Magdouli, Tarek Rouissi, and Satinder Kaur Brar. "Ex-situ biodegradation of petroleum hydrocarbons using Alcanivorax borkumensis enzymes." Biochemical Engineering Journal 132 (April 2018): 279–87. http://dx.doi.org/10.1016/j.bej.2018.01.014.

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Miri, Mandana, Bijan Bambai, Fatemeh Tabandeh, Majid Sadeghizadeh, and Nasrin Kamali. "Production of a recombinant alkane hydroxylase (AlkB2) from Alcanivorax borkumensis." Biotechnology Letters 32, no. 4 (December 2, 2009): 497–502. http://dx.doi.org/10.1007/s10529-009-0177-0.

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26

Hernández-López, Edna L., Jahaziel Gasperin, Johanna Bernáldez-Sarabia, Alexei F. Licea-Navarro, Abraham Guerrero, and Marcial Leonardo Lizárraga-Partida. "Detection of Alcanivorax spp., Cycloclasticus spp., and Methanomicrobiales in water column and sediment samples in the Gulf of Mexico by qPCR." Environmental Science and Pollution Research 26, no. 34 (November 3, 2019): 35131–39. http://dx.doi.org/10.1007/s11356-019-06551-7.

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Abstract Water column and sediment samples were collected in the southern Gulf of Mexico (GoMex) during 3 oceanographic cruises: XIXIMI-04 (September 2015), XIXIMI-05 (June 2016), and XIXIMI-06 (August 2017). DNA that was extracted from the samples was analyzed by qPCR to detect and quantify bacterial groups that have been reported to metabolize alkanes (Alcanivorax) and aromatic hydrocarbons (Cycloclasticus) and are involved in methane production (Methanomicrobiales). The results were then analyzed with regard to the water masses that are currently detected in the GoMex. Generally, we observed a decrease in the proportion of Alcanivorax and a rise in those of Cycloclasticus and Methanomicrobiales in samples from the surface to deep waters and in sediment samples. Scatterplots of the results showed that the relative abundance of the 3 groups was higher primarily from the surface to 1000 m, but the levels of Cycloclasticus and Methanomicrobiales were high in certain water samples below 1000 m and in sediments. In conclusion, oil-degrading bacteria are distributed widely from the surface to deep waters and sediments throughout the southern GoMex, representing a potential inoculum of bacteria for various hydrocarbon fractions that are ready for proliferation and degradation in the event of an oil spill from the seafloor or along the water column.
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Campos, FF, JE Garcia, CL Luna-Finkler, CC Davolos, MVF Lemos, and CD Pérez. "Alcanivorax dieselolei, an alkane-degrading bacterium associated with the mucus of the zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa)." Brazilian Journal of Biology 75, no. 2 (May 2015): 431–34. http://dx.doi.org/10.1590/1519-6984.16113.

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Analyses of 16S rDNA genes were used to identify the microbiota isolated from the mucus of the zoanthid Palythoa caribaeorum at Porto de Galinhas on the coast of Pernambuco State, Brazil. This study is important as the first report of this association, because of the potential biotechnological applications of the bacterium Alcanivorax dieselolei, and as evidence for the presence of a hydrocarbon degrading bacterium in a reef ecosystem such as Porto de Galinhas.
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28

Jacobs, Cheri Louise, Rodolpho do Aido-Machado, Carmien Tolmie, Martha Sophia Smit, and Diederik Johannes Opperman. "CYP153A71 from Alcanivorax dieselolei: Oxidation beyond Monoterminal Hydroxylation of n-Alkanes." Catalysts 12, no. 10 (October 11, 2022): 1213. http://dx.doi.org/10.3390/catal12101213.

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Selective oxyfunctionalization of non-activated C–H bonds remains a major challenge in synthetic chemistry. The biocatalytic hydroxylation of non-activated C–H bonds by cytochrome P450 monooxygenases (CYPs), however, offers catalysis with high regio- and stereoselectivity using molecular oxygen. CYP153s are a class of CYPs known for their selective terminal hydroxylation of n-alkanes and microorganisms, such as the bacterium Alcanivorax dieselolei, have evolved extensive enzymatic pathways for the oxyfunctionalization of various lengths of n-alkanes, including a CYP153 to yield medium-chain 1-alkanols. In this study, we report the characterization of the terminal alkane hydroxylase from A. dieselolei (CYP153A71) for the oxyfunctionalization of medium-chain n-alkanes in comparison to the well-known CYP153A6 and CYP153A13. Although the expected 1-alkanols are produced, CYP153A71 readily converts the 1-alkanols to the corresponding aldehydes, fatty acids, as well as α,ω-diols. CYP153A71 is also shown to readily hydroxylate medium-chain fatty acids. The X-ray crystal structure of CYP153A71 bound to octanoic acid is solved, yielding an insight into not only the regioselectivity, but also the binding orientation of the substrate, which can be used in future studies to evolve CYP153A71 for improved oxidations beyond terminal n-alkane hydroxylation.
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NAKANO, MIYO, SUGURU OKUNISHI, REIJI TANAKA, and HIROTO MAEDA. "Denitrifying Activity and Homologous Enzyme Analysis of Alcanivorax dieselolei Strain N1203." Biocontrol Science 14, no. 3 (2009): 97–105. http://dx.doi.org/10.4265/bio.14.97.

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30

Schneiker, Susanne, Vítor AP Martins dos Santos, Daniela Bartels, Thomas Bekel, Martina Brecht, Jens Buhrmester, Tatyana N. Chernikova, et al. "Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Nature Biotechnology 24, no. 8 (July 30, 2006): 997–1004. http://dx.doi.org/10.1038/nbt1232.

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31

Abraham, Wolf-Rainer, Holger Meyer, and Misha Yakimov. "Novel glycine containing glucolipids from the alkane using bacterium Alcanivorax borkumensis." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 1393, no. 1 (July 1998): 57–62. http://dx.doi.org/10.1016/s0005-2760(98)00058-7.

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32

Hara, Akihiro, Sang-ho Baik, Kazuaki Syutsubo, Norihiko Misawa, Theo H. M. Smits, Jan B. van Beilen, and Shigeaki Harayama. "Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2." Environmental Microbiology 6, no. 3 (March 2004): 191–97. http://dx.doi.org/10.1046/j.1462-2920.2003.00550.x.

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33

Warr, Laurence N., Maria Schlüter, Frieder Schauer, Gregory M. Olson, Laura M. Basirico, and Ralph J. Portier. "Nontronite-enhanced biodegradation of Deepwater Horizon crude oil by Alcanivorax borkumensis." Applied Clay Science 158 (June 2018): 11–20. http://dx.doi.org/10.1016/j.clay.2018.03.011.

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34

Kadri, Tayssir, Tarek Rouissi, Sara Magdouli, Satinder Kaur Brar, Krishnamoorthy Hegde, Zied Khiari, Rimeh Daghrir, and Jean-Marc Lauzon. "Production and characterization of novel hydrocarbon degrading enzymes from Alcanivorax borkumensis." International Journal of Biological Macromolecules 112 (June 2018): 230–40. http://dx.doi.org/10.1016/j.ijbiomac.2018.01.177.

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35

Galkin, B. N., M. O. Finogenova, А. S. Semenets, M. B. Galkin, and T. O. Filipova. "BIOSURFACTANTS FROM MARINE MICROORGANISMS: I. STRUCTURE AND FUNCTIONS." Microbiology&Biotechnology, no. 3(53) (December 19, 2021): 6–27. http://dx.doi.org/10.18524/2307-4663.2021.3(53).242877.

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Marine microorganisms have unique metabolic and physiological characteristics and are an important source of new biomolecules such as biosurfactants. Low molecular weight surfactants are glycolipids, phospholipids, fatty acids, lipopeptides and lipoproteins, and high molecular weight surfactants are mixtures of heteropolysaccharides, lipopolysaccharides, lipoproteins and proteins. The main general of bacteria that synthesize biosurfactants are Pseudomonas, Bacillus, Acinetobacter, Antarctobacter, Rhodococcus, Halomonas, Alcanivorax, Pseudoalteromonas and Marinobacter. This review examines the structure and function of biosurfactants isolated from marine microorganisms.
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36

Al-Wahaib, Dhuha, Dhia Al-Bader, Dana K. Al-Shaikh Abdou, Mohamed Eliyas, and Samir S. Radwan. "Consistent Occurrence of Hydrocarbonoclastic Marinobacter Strains in Various Cultures of Picocyanobacteria from the Arabian Gulf: Promising Associations for Biodegradation of Marine Oil Pollution." Journal of Molecular Microbiology and Biotechnology 26, no. 4 (2016): 261–68. http://dx.doi.org/10.1159/000445686.

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Fifteen nonaxenic cultures of picocyanobacteria were isolated from the Arabian Gulf, from which 122 heterotrophic bacterial strains were obtained. Based on their 16S rRNA gene sequences, those strains were affiliated with 22 different species, 82.8% of which belonged to the genus <i>Marinobacter</i>, known to comprise hydrocarbonoclastic strains. The remaining species belonged to the genera <i>Alcanivorax, Bacillus, Halomonas, Mesorhizobium, and Paenibacillus, </i>and a Bacteriodetes bacterium also known to comprise hydrocarbonoclastic strains. All the picocyanobacterial cultures harbored one or more strains of <i>Marinobacter</i>. <i>Marinobacter</i> in addition to <i>Alcanivorax</i> and other genera isolated from those picocyanobacteria grew on Tween 80, crude oil, and pure hydrocarbons as sole sources of carbon and energy, i.e. they are related to the obligate hydrocarbonoclastic bacteria group. They consumed crude oil, <i>n</i>-octadecane, and phenanthrene in batch cultures. The results indicated that <i>Marinobacter</i> isolates seemed to grow better and consume more oil in the presence of their host picocyanobacteria than in their absence. Such natural microbial associations assumingly play a role in bioremediation of spilled hydrocarbons in the Arabian Gulf. Similar associations probably occur in other marine environments as well and are active in oil spill removal.
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Chen, Shuang, Da-shan Zhang, and Jin-hui Wang. "Antimycic Acid and its Acetyl Derivative from Deep-sea-derived Alcanivorax sp. SHA4 with Neuroprotective Properties." Natural Product Communications 18, no. 1 (January 2023): 1934578X2211106. http://dx.doi.org/10.1177/1934578x221110661.

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Chemical investigation of secondary metabolites of the deep-sea-derived Alcanivorax sp. SHA4 identified a new compound 1 which was antimycic acid (2)'s acetyl derivative, and 11 known compounds (2-12). Their structures were elucidated by extensive nuclear magnetic resonance and mass spectrometry spectroscopic analyses, and the absolute configuration of compound 1 was determined by Marfey's method. Bioactivity assays indicated that compounds 1 and 2 exhibited significant neuroprotective properties against glutamate-induced PC12 cell death in 0.02-0.31 μM.
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38

Alghuthaymi, Mousa A., Ahmed M. Awad, and Hamdy A. Hassan. "Isolation and Characterization a Novel Catabolic Gene Cluster Involved in Chlorobenzene Degradation in Haloalkaliphilic Alcanivorax sp. HA03." Biology 11, no. 5 (May 9, 2022): 724. http://dx.doi.org/10.3390/biology11050724.

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Chlorobenzene (CB) poses a serious risk to human health and the environment, and because of its low degradation rate by microorganisms, it persists in the environment. Some bacterial strains can use CB as growth substrates and their degradative pathways have evolved; very little is known about these pathways and the enzymes for CB degradation in high pH and salinity environments. Alcanivorax sp. HA03 was isolated from the extremely saline and alkaline site. HA03 has the capability to degrade benzene, toluene and chlorobenzene (CB). CB catabolic genes were isolated from HA03, which have a complete gene cluster comprising α and β subunits, ferredoxin and ferredoxin reductase (CBA1A2A3A4), as well as one gene-encoding enzyme for chlorocatechol 1,2-dioxygenase (CC12DOs). Based on the deduced amino acid sequence homology, the gene cluster was thought to be responsible for the upper and lower catabolic pathways of CB degradation. The CBA1A2A3A4 genes probably encoding a chlorobenzene dioxygenase was confirmed by expression during the growth on CB by RT-PCR. Heterologous expression revealed that CBA1A2A3A4 exhibited activity for CB transformation into 3-chlorocatechol, while CC12DOs catalyze 3-chlorocatechol, transforming it into 2-chloromucounate. SDS-PAGE analysis indicated that the sizes of CbA1 and (CC12DOs) gene products were 51.8, 27.5 kDa, respectively. Thus, Alcanivorax sp. HA03 constitutes the first bacterial strain described in the metabolic pathway of CB degradation under high pH and salinity conditions. This finding may have obvious potential for the bioremediation of CB in both highly saline and alkaline contaminated sites.
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39

Darmayati, Yeti, Shigeaki Harayama, Atsushi Yamazoe, Ariani Hatmanti, Sulistiani, Ruyitno Nuchsin, and Djoko Hadi Kunarso. "HYDROCARBONOCLASTIC BACTERIA FROM JAKARTA BAY AND SERIBU ISLANDS." Marine Research in Indonesia 33, no. 1 (June 30, 2008): 55–64. http://dx.doi.org/10.14203/mri.v33i1.506.

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Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.It’s sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.
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Li, Anzhang, and Zongze Shao. "Biochemical Characterization of a Haloalkane Dehalogenase DadB from Alcanivorax dieselolei B-5." PLoS ONE 9, no. 2 (February 28, 2014): e89144. http://dx.doi.org/10.1371/journal.pone.0089144.

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Liu, Jiwen, Qiaomeng Ren, Yunhui Zhang, Yuying Li, Xiaorong Tian, Yanhong Wu, Jiwei Tian, and Xiao-Hua Zhang. "Alcanivorax profundi sp. nov., isolated from deep seawater of the Mariana Trench." International Journal of Systematic and Evolutionary Microbiology 69, no. 2 (February 1, 2019): 371–76. http://dx.doi.org/10.1099/ijsem.0.003145.

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42

Sabirova, Julia S., Manuel Ferrer, Daniela Regenhardt, Kenneth N. Timmis, and Peter N. Golyshin. "Proteomic Insights into Metabolic Adaptations in Alcanivorax borkumensis Induced by Alkane Utilization." Journal of Bacteriology 188, no. 11 (June 1, 2006): 3763–73. http://dx.doi.org/10.1128/jb.00072-06.

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ABSTRACT Alcanivorax borkumensis is a ubiquitous marine petroleum oil-degrading bacterium with an unusual physiology specialized for alkane metabolism. This “hydrocarbonoclastic” bacterium degrades an exceptionally broad range of alkane hydrocarbons but few other substrates. The proteomic analysis presented here reveals metabolic features of the hydrocarbonoclastic lifestyle. Specifically, hexadecane-grown and pyruvate-grown cells differed in the expression of 97 cytoplasmic and membrane-associated proteins whose genes appeared to be components of 46 putative operon structures. Membrane proteins up-regulated in alkane-grown cells included three enzyme systems able to convert alkanes via terminal oxidation to fatty acids, namely, enzymes encoded by the well-known alkB1 gene cluster and two new alkane hydroxylating systems, a P450 cytochrome monooxygenase and a putative flavin-binding monooxygenase, and enzymes mediating β-oxidation of fatty acids. Cytoplasmic proteins up-regulated in hexadecane-grown cells reflect a central metabolism based on a fatty acid diet, namely, enzymes of the glyoxylate bypass and of the gluconeogenesis pathway, able to provide key metabolic intermediates, like phosphoenolpyruvate, from fatty acids. They also include enzymes for synthesis of riboflavin and of unsaturated fatty acids and cardiolipin, which presumably reflect membrane restructuring required for membranes to adapt to perturbations induced by the massive influx of alkane oxidation enzymes. Ancillary functions up-regulated included the lipoprotein releasing system (Lol), presumably associated with biosurfactant release, and polyhydroxyalkanoate synthesis enzymes associated with carbon storage under conditions of carbon surfeit. The existence of three different alkane-oxidizing systems is consistent with the broad range of oil hydrocarbons degraded by A. borkumensis and its ecological success in oil-contaminated marine habitats.
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Lyu, Li-Na, Haibing Ding, Zhisong Cui, and David L. Valentine. "The Wax–Liquid Transition Modulates Hydrocarbon Respiration Rates in Alcanivorax borkumensis SK2." Environmental Science & Technology Letters 5, no. 5 (April 17, 2018): 277–82. http://dx.doi.org/10.1021/acs.estlett.8b00143.

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Bookstaver, Michelle, Michael P. Godfrin, Arijit Bose, and Anubhav Tripathi. "An insight into the growth of Alcanivorax borkumensis under different inoculation conditions." Journal of Petroleum Science and Engineering 129 (May 2015): 153–58. http://dx.doi.org/10.1016/j.petrol.2015.02.038.

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Manilla-Pérez, Efraín, Alvin Brian Lange, Heinrich Luftmann, Horst Robenek, and Alexander Steinbüchel. "Neutral lipid production in Alcanivorax borkumensis SK2 and other marine hydrocarbonoclastic bacteria." European Journal of Lipid Science and Technology 113, no. 1 (November 17, 2010): 8–17. http://dx.doi.org/10.1002/ejlt.201000374.

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46

Dastgheib, Seyed Mohammad Mehdi, Mohamad Ali Amoozegar, Khosro Khajeh, and Antonio Ventosa. "A halotolerant Alcanivorax sp. strain with potential application in saline soil remediation." Applied Microbiology and Biotechnology 90, no. 1 (December 14, 2010): 305–12. http://dx.doi.org/10.1007/s00253-010-3049-6.

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47

Cappello, Simone, Renata Denaro, Maria Genovese, Laura Giuliano, and Michail M. Yakimov. "Predominant growth of Alcanivorax during experiments on “oil spill bioremediation” in mesocosms." Microbiological Research 162, no. 2 (April 2007): 185–90. http://dx.doi.org/10.1016/j.micres.2006.05.010.

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48

Wang, Yong, Tie Gang Li, Meng Ying Wang, Qi Liang Lai, Jiang Tao Li, Zhao Ming Gao, Zong Ze Shao, and Pei-Yuan Qian. "Archive of bacterial community in anhydrite crystals from a deep-sea basin provides evidence of past oil-spilling in a benthic environment in the Red Sea." Biogeosciences 13, no. 23 (November 30, 2016): 6405–17. http://dx.doi.org/10.5194/bg-13-6405-2016.

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Abstract. In deep-sea sediment, the microbes present in anhydrite crystals are potential markers of the past environment. In the Atlantis II Deep, anhydrite veins were produced by mild mixture of calcium-rich hydrothermal solutions and sulfate in the bottom water, which had probably preserved microbial inhabitants in the past seafloor of the Red Sea. In this study, this hypothesis was tested by analyzing the metagenome of an anhydrite crystal sample from the Atlantis II Deep. The estimated age of the anhydrite layer was between 750 and 770 years, which might span the event of hydrothermal eruption into the benthic floor. The 16S/18S rRNA genes in the metagenome were assigned to bacteria, archaea, fungi and even invertebrate species. The dominant species in the crystals was an oil-degrading Alcanivorax borkumensis bacterium, which was not detected in the adjacent sediment layer. Fluorescence microscopy using 16S rRNA and marker gene probes revealed intact cells of the Alcanivorax bacterium in the crystals. A draft genome of A. borkumensis was binned from the metagenome. It contained all functional genes for alkane utilization and the reduction of nitrogen oxides. Moreover, the metagenomes of the anhydrites and control sediment contained aromatic degradation pathways, which were mostly derived from Ochrobactrum sp. Altogether, these results indicate an oxic, oil-spilling benthic environment in the Atlantis II basin of the Red Sea in approximately the 14th century. The original microbial inhabitants probably underwent a dramatic selection process via drastic environmental changes following the formation of an overlying anoxic brine pool in the basin due to hydrothermal activities.
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Cappello, Simone, Maria Genovese, Renata Denaro, Santina Santisi, Anna Volta, Martina Bonsignore, Giuseppe Mancini, Laura Giuliano, Lucrezia Genovese, and Michail M. Yakimov. "Quick stimulation of Alcanivorax sp. by bioemulsificant EPS2003 on microcosm oil spill simulation." Brazilian Journal of Microbiology 45, no. 4 (December 2014): 1317–23. http://dx.doi.org/10.1590/s1517-83822014000400023.

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Zadjelovic, Vinko, Audam Chhun, Mussa Quareshy, Eleonora Silvano, Juan R. Hernandez‐Fernaud, María M. Aguilo‐Ferretjans, Rafael Bosch, Cristina Dorador, Matthew I. Gibson, and Joseph A. Christie‐Oleza. "Beyond oil degradation: enzymatic potential of Alcanivorax to degrade natural and synthetic polyesters." Environmental Microbiology 22, no. 4 (April 2020): 1356–69. http://dx.doi.org/10.1111/1462-2920.14947.

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