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1

Craggs, R. J., S. Heubeck, T. J. Lundquist, and J. R. Benemann. "Algal biofuels from wastewater treatment high rate algal ponds." Water Science and Technology 63, no. 4 (February 1, 2011): 660–65. http://dx.doi.org/10.2166/wst.2011.100.

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This paper examines the potential of algae biofuel production in conjunction with wastewater treatment. Current technology for algal wastewater treatment uses facultative ponds, however, these ponds have low productivity (∼10 tonnes/ha.y), are not amenable to cultivating single algal species, require chemical flocculation or other expensive processes for algal harvest, and do not provide consistent nutrient removal. Shallow, paddlewheel-mixed high rate algal ponds (HRAPs) have much higher productivities (∼30 tonnes/ha.y) and promote bioflocculation settling which may provide low-cost algal harvest. Moreover, HRAP algae are carbon-limited and daytime addition of CO2 has, under suitable climatic conditions, the potential to double production (to ∼60 tonnes/ha.y), improve bioflocculation algal harvest, and enhance wastewater nutrient removal. Algae biofuels (e.g. biogas, ethanol, biodiesel and crude bio-oil), could be produced from the algae harvested from wastewater HRAPs, The wastewater treatment function would cover the capital and operation costs of algal production, with biofuel and recovered nutrient fertilizer being by-products. Greenhouse gas abatement results from both the production of the biofuels and the savings in energy consumption compared to electromechanical treatment processes. However, to achieve these benefits, further research is required, particularly the large-scale demonstration of wastewater treatment HRAP algal production and harvest.
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2

Koçer, Anıl Tevfik, and Didem Özçimen. "Investigation of the biogas production potential from algal wastes." Waste Management & Research: The Journal for a Sustainable Circular Economy 36, no. 11 (September 25, 2018): 1100–1105. http://dx.doi.org/10.1177/0734242x18798447.

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In recent years, researchers focused their attention on biogas production more than ever to meet the energy demand. Especially, biogas obtained from algal wastes has become a trending research area owing to the high content of volatile solids in algae. The main purpose of this study is to determine the biogas production potential from algal wastes and examine the effect of temperature and particle size parameters on biogas yield. A comparison was made between the biogas production potential of microalgal wastes, obtained after oil extraction, and macroalgal wastes collected from coastal areas. It was found that algal biogas yield is directly proportional to temperature and inversely proportional to particle size. Optimal conditions for biogas production from algal wastes were determined as the temperature of 55 °C, a particle size of 200 μm, a residence time of 30 days and an alga–inoculum ratio of 1:4 (w:w). Highest biogas yield obtained under these conditions was found as 342.59 cm3 CH4 g−1 VS with Ulva lactuca. Under thermophilic conditions, both micro- and macroalgal biogas yields were comparable. It can be concluded that algal biomass is a good source for biogas production, although further research is needed to increase biogas yield and quality.
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3

Reis, Marcello, Maria Elisa Marciano Martinez, and Alexandre Guimarães Vasconcellos. "PROSPECTIVE ANALYSIS OF ALGAL BIODIESEL PRODUCTION." Journal of Mechatronics Engineering 4, no. 2 (September 21, 2021): 12–18. http://dx.doi.org/10.21439/jme.v4i2.97.

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This article aims to carry out an initial patent mapping of algal biodiesel. The production of algal biodiesel is one of the forms of third generation biodiesel; it is an environmentally friendly alternative energy whose main advantage is that it does not compete with food, as the algal biodiesel is produced from synthesized lipids by algae in growth using sunlight. The methodology used was the patent mapping by activity having as search criteria: the Espacenet database (“worldwide”); and, the keyword: biodiesel and algae and algal biodiesel. It was observed that about 80% of the family of patent documents referring to this technology were applied between 2007 and 2016 and that these documents were published mainly in China (34% of patent documents), followed by the United States (25% of patent documents) and thirdly, the World Intellectual Property Organization (WO), that is, the PCT's international patent application, which indicates an interest in protection in several countries (15% of patent documents). Concluding that China and the United States are the countries that invest the most in the development and protection of technologies related to the production of algal biodiesel, however, the interest in protection goes beyond these countries, since the interest in alternative energies is worldwide.
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4

Bošnjaković, Mladen, and Nazaruddin Sinaga. "The Perspective of Large-Scale Production of Algae Biodiesel." Applied Sciences 10, no. 22 (November 18, 2020): 8181. http://dx.doi.org/10.3390/app10228181.

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We have had high expectations for using algae biodiesel for many years, but the quantities of biodiesel currently produced from algae are tiny compared to the quantities of conventional diesel oil. Furthermore, no comprehensive analysis of the impact of all factors on the market production of algal biodiesel has been made so far. This paper aims to analyze the strengths, weaknesses, opportunities, and threats associated with algal biodiesel, to evaluate its production prospects for the biofuels market. The results of the analysis show that it is possible to increase the efficiency of algae biomass production further. However, because the production of this biodiesel is an energy-intensive process, the price of biodiesel is high. Opportunities for more economical production of algal biodiesel are seen in integration with other processes, such as wastewater treatment, but this does not ensure large-scale production. The impact of state policies and laws is significant in the future of algal biodiesel production. With increasingly stringent environmental requirements, electric cars are a significant threat to biodiesel production. By considering all the influencing factors, it is not expected that algal biodiesel will gain an essential place in the fuel market.
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5

Park, J. B. K., and R. J. Craggs. "Wastewater treatment and algal production in high rate algal ponds with carbon dioxide addition." Water Science and Technology 61, no. 3 (February 1, 2010): 633–39. http://dx.doi.org/10.2166/wst.2010.951.

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High rate algal ponds (HRAPs) provide improved wastewater treatment over conventional wastewater stabilisation ponds; however, algal production and recovery of wastewater nutrients as algal biomass is limited by the low carbon:nitrogen ratio of wastewater. This paper investigates the influence of CO2 addition (to augment daytime carbon availability) on wastewater treatment performance and algal production of two pilot-scale HRAPs operated with different hydraulic retention times (4 and 8 days) over a New Zealand Summer (November–March, 07/08). Weekly measurements were made of influent and effluent flow rate and water qualities, algal and bacterial biomass production, and the percentage of algae biomass harvested in gravity settling units. This research shows that the wastewater treatment HRAPs with CO2 addition achieved a mean algal productivity of 16.7 g/m2/d for the HRAP4d (4 d HRT, maximum algae productivity of 24.7 g/m2/d measured in January 08) and 9.0 g/m2/d for the HRAP8d (8 d HRT)). Algae biomass produced in the HRAPs was efficiently harvested by simple gravity settling units (mean harvested algal productivity: 11.5 g/m2/d for the HRAP4d and 7.5 g/m2/d for the HRAP8d respectively). Higher bacterial composition and the larger size of algal/bacterial flocs of the HRAP8d biomass increased harvestability (83%) compared to that of HRAP4d biomass (69%).
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6

Park, J. B. K., and R. J. Craggs. "Effect of algal recycling rate on the performance of Pediastrum boryanum dominated wastewater treatment high rate algal pond." Water Science and Technology 70, no. 8 (August 23, 2014): 1299–306. http://dx.doi.org/10.2166/wst.2014.369.

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Recycling a portion of gravity harvested algae promoted the dominance of a rapidly settling colonial alga, Pediastrum boryanum (P. boryanum) and improved both biomass productivity and settleability in High Rate Algal Pond (HRAP) treating domestic wastewater. The effect of algal recycling rate on HRAP performance was investigated using 12 replicate mesocosms (18 L) that were operated semi-continuously under ambient conditions. Three experiments were conducted during different seasons with each experiment lasting up to 36 days. Recycling 10%, 25%, and 50% of the ‘mass’ of daily algal production all increased total biomass concentration in the mesocosms. However, recycling >10% reduced the organic content (volatile suspended solids (VSS)) of the mesocosm biomass from 83% to 68% and did not further increase biomass productivity (based on VSS). This indicates that if a HRAP is operated with a low algal concentration and does not utilise all the available sunlight, algal recycling increases the algal concentration up to an optimum level, resulting in higher algal biomass productivity. Recycling 10% of the daily algal production not only increased biomass productivity by ∼40%, but increased biomass settleability by ∼25%, which was probably a consequence of the ∼30% increase in P. boryanum dominance in the mesocosms compared with controls without recycling.
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7

Jian, Hou, Yang Jing, and Zhang Peidong. "Life Cycle Analysis on Fossil Energy Ratio of Algal Biodiesel: Effects of Nitrogen Deficiency and Oil Extraction Technology." Scientific World Journal 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/920968.

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Life cycle assessment (LCA) has been widely used to analyze various pathways of biofuel preparation from “cradle to grave.” Effects of nitrogen supply for algae cultivation and technology of algal oil extraction on life cycle fossil energy ratio of biodiesel are assessed in this study. Life cycle fossil energy ratio ofChlorella vulgarisbased biodiesel is improved by growing algae under nitrogen-limited conditions, while the life cycle fossil energy ratio of biodiesel production fromPhaeodactylum tricornutumgrown with nitrogen deprivation decreases. Compared to extraction of oil from dried algae, extraction of lipid from wet algae with subcritical cosolvents achieves a 43.83% improvement in fossil energy ratio of algal biodiesel when oilcake drying is not considered. The outcome for sensitivity analysis indicates that the algal oil conversion rate and energy content of algae are found to have the greatest effects on the LCA results of algal biodiesel production, followed by utilization ratio of algal residue, energy demand for algae drying, capacity of water mixing, and productivity of algae.
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8

Evans, Marlene S., Richard D. Robarts, and Michael T. Arts. "Predicted versus actual determinations of algal production, algal biomass, and zooplankton biomass in a hypereutrophic, hyposaline prairie lake." Canadian Journal of Fisheries and Aquatic Sciences 52, no. 5 (May 1, 1995): 1037–49. http://dx.doi.org/10.1139/f95-102.

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We compared the accuracy of various regression models in predicting algal production, algal biomass and composition, and zooplankton biomass in a hypereutrophic, hyposaline prairie lake. The total phosphorus (TP) models investigated underestimated mean summer algal biomass and inedible biomass: the models overestimated mean summer edible algae biomass and annual primary production in the euphotic zone. Differences between predicted and actual biomass values are attributed to intense zooplankton grazing on the edible algal community and to the gradual accumulation of slow-growing, inedible algae. The TP model investigated provided an accurate prediction of zooplankton biomass. The algal biomass model overestimated zooplankton biomass, possibly because edible algae accounted for a very small fraction of algal biomass in Humboldt Lake during the ice-free season. The chlorophyll model investigated underestimated zooplankton biomass, apparently because Humboldt Lake algae have a relatively low chlorophyll content. The use of a 0.01 conversion factor to estimate algal biomass on the basis of chlorophyll appears to be inadequate and requires further study. There was no evidence that hyposaline Humboldt Lake has a relatively high zooplankton to phytoplankton biomass ratio when compared with freshwater lakes.
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9

Czerwik-Marcinkowska, Joanna, Katarzyna Gałczyńska, Jerzy Oszczudłowski, Andrzej Massalski, Jacek Semaniak, and Michał Arabski. "Fatty Acid Methyl Esters of the Aerophytic Cave Alga Coccomyxa subglobosa as a Source for Biodiesel Production." Energies 13, no. 24 (December 9, 2020): 6494. http://dx.doi.org/10.3390/en13246494.

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The microscopic alga Coccomyxa subglobosa, collected from the Głowoniowa Nyża Cave (Tatra Mountains, Poland), is a source of fatty acids (FAs) that could be used for biodiesel production. FAs from subaerial algae have unlimited availability because of the ubiquity of algae in nature. Algal culture was carried out under laboratory conditions and algal biomass was measured during growth phase, resulting in 5 g of dry weight (32% oil). The fatty acid methyl ester (FAME) profile was analyzed by means of gas chromatography–mass spectrometry (GC–MS). The presence of lipids and chloroplasts in C. subglobosa was demonstrated using GC–MS and confocal laser microscopy. Naturally occurring FAMEs contained C12–C24 compounds, and methyl palmitate (28.5%) and methyl stearate (45%) were the predominant lipid species. Aerophytic algae could be an important component of biodiesel production, as they are omnipresent and environmentally friendly, contain more methyl esters than seaweed, and can be easily produced on a large scale.
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10

Shurin, Jonathan B., Michael D. Burkart, Stephen P. Mayfield, and Val H. Smith. "Recent progress and future challenges in algal biofuel production." F1000Research 5 (October 4, 2016): 2434. http://dx.doi.org/10.12688/f1000research.9217.1.

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Modern society is fueled by fossil energy produced millions of years ago by photosynthetic organisms. Cultivating contemporary photosynthetic producers to generate energy and capture carbon from the atmosphere is one potential approach to sustaining society without disrupting the climate. Algae, photosynthetic aquatic microorganisms, are the fastest growing primary producers in the world and can therefore produce more energy with less land, water, and nutrients than terrestrial plant crops. We review recent progress and challenges in developing bioenergy technology based on algae. A variety of high-value products in addition to biofuels can be harvested from algal biomass, and these may be key to developing algal biotechnology and realizing the commercial potential of these organisms. Aspects of algal biology that differentiate them from plants demand an integrative approach based on genetics, cell biology, ecology, and evolution. We call for a systems approach to research on algal biotechnology rooted in understanding their biology, from the level of genes to ecosystems, and integrating perspectives from physical, chemical, and social sciences to solve one of the most critical outstanding technological problems.
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11

K., Santhoshkumar, Prasanthkumar S., and J. G. Ray. "Chlorococcum humicola (Nageli) Rabenhorst as a Renewable Source of Bioproducts and Biofuel." Journal of Plant Studies 5, no. 1 (February 29, 2016): 48. http://dx.doi.org/10.5539/jps.v5n1p48.

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Among the diverse new generation biomass yielding species, green algae are the most promising organisms. Compared to biomass production of other organisms, production of algae is less laborious, quite fast, and more economical. Moreover, eutrophicated waters get naturally purified in the cultivation process of algae. Algal biomass from monoculture of specific species, which are rich in carbohydrates, proteins and lipids, is considered a good source of diverse bio-products and feed-stock for food, feeds and bio-fuels. Quantity and quality of algal biomass for specific products depend on the species and strains as well as environmental conditions of cultivation. In this connection, biomass productivity and oil-yield of a local strain of <em>Chlorococcum humicola </em>(Nageli) Rabenhorst was assessed in Bold’s Basal Medium. Long-term storage capacity of the alga was tried by entrapping the algal cells in sodium alginate beads, which showed viability up to 14 months. Estimation of total carbohydrate, protein, lipid and chemical characterization of oil as well as the feasibility of its conversion to biodiesel revealed the industrial potential of this local strain as a source of food and biofuel. Fatty acid profiling of the extracted oil showed that 70% are mono-saturated and 12.2 % are nutritionally important polyunsaturated fatty acids. The oil could be effectively trans-esterified to methyl esters and the conversion was confirmed by FTIR spectroscopy. Further standardization of the mass production of the alga in natural environmental conditions for biomass and oil is progressing to optimize its value as globally competent food, nutraceutical and biofuel resource.
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12

Patyshakuliyeva, Aleksandrina, Daniel L. Falkoski, Ad Wiebenga, Klaas Timmermans, and Ronald P. de Vries. "Macroalgae Derived Fungi Have High Abilities to Degrade Algal Polymers." Microorganisms 8, no. 1 (December 26, 2019): 52. http://dx.doi.org/10.3390/microorganisms8010052.

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Marine fungi associated with macroalgae are an ecologically important group that have a strong potential for industrial applications. In this study, twenty-two marine fungi isolated from the brown seaweed Fucus sp. were examined for their abilities to produce algal and plant biomass degrading enzymes. Growth of these isolates on brown and green algal biomass revealed a good growth, but no preference for any specific algae. Based on the analysis of enzymatic activities, macroalgae derived fungi were able to produce algae specific and (hemi-)cellulose degrading enzymes both on algal and plant biomass. However, the production of algae specific activities was lower than the production of cellulases and xylanases. These data revealed the presence of different enzymatic approaches for the degradation of algal biomass by macroalgae derived fungi. In addition, the results of the present study indicate our poor understanding of the enzymes involved in algal biomass degradation and the mechanisms of algal carbon source utilization by marine derived fungi.
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13

Saad, Marwa G., Noura S. Dosoky, Mohamed S. Zoromba, and Hesham M. Shafik. "Algal Biofuels: Current Status and Key Challenges." Energies 12, no. 10 (May 20, 2019): 1920. http://dx.doi.org/10.3390/en12101920.

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The current fossil fuel reserves are not sufficient to meet the increasing demand and very soon will become exhausted. Pollution, global warming, and inflated oil prices have led the quest for renewable energy sources. Algal biofuels represent a potential source of renewable energy. Algae, as the third generation feedstock, are suitable for biodiesel and bioethanol production due to their quick growth, excellent biomass yield, and high lipid and carbohydrate contents. With their huge potential, algae are expected to surpass the first and second generation feedstocks. Only a few thousand algal species have been investigated as possible biofuel sources, and none of them was ideal. This review summarizes the current status of algal biofuels, important steps of algal biofuel production, and the major commercial production challenges.
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14

Wang, J., and AE Douglas. "Nitrogen recycling or nitrogen conservation in an alga-invertebrate symbiosis?" Journal of Experimental Biology 201, no. 16 (August 15, 1998): 2445–53. http://dx.doi.org/10.1242/jeb.201.16.2445.

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When corals and allied animals are deprived of their symbiotic algae, the ammonium content in their tissues rises. This is commonly interpreted as evidence for nitrogen recycling (i.e. algal assimilation of animal waste ammonium into amino acids that are released back to the animal), but it can also be explained as nitrogen conservation by the animal (i.e. reduced net ammonium production in response to the receipt of algal photosynthetic carbon). This study discriminated between these interpretations in two ways. First, the increased ammonium concentration in the sea anemone Aiptasia pulchella, caused by darkness or depletion of the alga Symbiodinium, was partially or completely reversed by supplementing the medium with organic carbon compounds (e.g. &lt;IMG src="/images/symbols/&agr ;.gif" WIDTH="9" HEIGHT="12" ALIGN= "BOTTOM" NATURALSIZEFLAG="3"&gt;-ketoglutarate). Second, the activity of the ammonium-assimilating enzyme glutamine synthetase and the concentration of protein amino acids in the free amino acid pool of the animal, which were depressed by darkness and algal depletion, were restored by exogenous carbon compounds. It is concluded that organic carbon, whether derived from algal photosynthate or exogenously, promotes the animal's capacity for ammonium assimilation and reduces ammonium production from amino acid degradation. These processes contribute to nitrogen conservation in the animal, but they confound the interpretation of various studies on nitrogen recycling by symbiotic algae.
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15

Ishizaki, Riaru, Ryozo Noguchi, Agusta Samodra Putra, Sosaku Ichikawa, Tofael Ahamed, and Makoto M. Watanabe. "Reduction in Energy Requirement and CO2 Emission for Microalgae Oil Production Using Wastewater." Energies 13, no. 7 (April 2, 2020): 1641. http://dx.doi.org/10.3390/en13071641.

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A comparative evaluation of energy requirement and CO2 emission was performed for native polyculture microalgae oil production in a wastewater treatment plant (WWTP). The wastewater provided nutrients for algae growth. Datasets of microalgae oil production and their details were collected from the Minamisoma pilot plant. Environmental impact estimation from direct energy and material balance was analyzed using SimaPro® v8.0.4. in two scenarios: existing and algal scenarios. In the existing scenario, CO2 emission sources were from wastewater treatment, sludge treatment, and import of crude oil. In the algal scenario, CO2 emission with microalgae production was considered using wastewater treatment, CO2 absorption from growing algae, and hydrothermal liquefaction (HTL) for extraction, along with the exclusion of exhausted CO2 emission for growing algae and use of discharged heat for HTL. In these two scenarios, 1 m3 of wastewater was treated, and 2.17 MJ higher heating value (HHV) output was obtained. Consequently, 2.76 kg-CO2 eq/m3-wastewater in the existing scenario and 1.59 kg-CO2 eq/m3-wastewater in the algal scenario were calculated. In the HTL process, 21.5 MJ/m3-wastewater of the discharged heat energy was required in the algal scenario. Hence, the efficiency of the biocrude production system will surpass those of the WWTP and imported crude oil.
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16

Dorrell, Richard G., and Alison G. Smith. "Do Red and Green Make Brown?: Perspectives on Plastid Acquisitions within Chromalveolates." Eukaryotic Cell 10, no. 7 (May 27, 2011): 856–68. http://dx.doi.org/10.1128/ec.00326-10.

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ABSTRACT The chromalveolate “supergroup” is of key interest in contemporary phycology, as it contains the overwhelming majority of extant algal species, including several phyla of key importance to oceanic net primary productivity such as diatoms, kelps, and dinoflagellates. There is also intense current interest in the exploitation of these algae for industrial purposes, such as biodiesel production. However, the evolution of the constituent species, and in particular the origin and radiation of the chloroplast genomes, remains poorly understood. In this review, we discuss current theories of the origins of the extant red alga-derived chloroplast lineages in the chromalveolates and the potential ramifications of the recent discovery of large numbers of green algal genes in chromalveolate genomes. We consider that the best explanation for this is that chromalveolates historically possessed a cryptic green algal endosymbiont that was subsequently replaced by a red algal chloroplast. We consider how changing selective pressures acting on ancient chromalveolate lineages may have selectively favored the serial endosymbioses of green and red algae and whether a complex endosymbiotic history facilitated the rise of chromalveolates to their current position of ecological prominence.
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17

Zhang, Liping, and Anastasios Melis. "Probing green algal hydrogen production." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 357, no. 1426 (October 29, 2002): 1499–509. http://dx.doi.org/10.1098/rstb.2002.1152.

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The recently developed two–stage photosynthesis and H 2 –production protocol with green algae is further investigated in this work. The method employs S deprivation as a tool for the metabolic regulation of photosynthesis. In the presence of S, green algae perform normal photosynthesis, carbohydrate accumulation and oxygen production. In the absence of S, normal photosynthesis stops and the algae slip into the H 2 –production mode. For the first time, to our knowledge, significant amounts of H 2 gas were generated, essentially from sunlight and water. Rates of H 2 production could be sustained continuously for ca . 80 h in the light, but gradually declined thereafter. This work examines biochemical and physiological aspects of this process in the absence or presence of limiting amounts of S nutrients. Moreover, the effects of salinity and of uncouplers of phosphorylation are investigated. It is shown that limiting levels of S can sustain intermediate levels of oxygenic photosynthesis, in essence raising the prospect of a calibration of the rate of photosynthesis by the S content in the growth medium of the algae. It is concluded that careful titration of the supply of S nutrients in the green alga medium might permit the development of a continuous H 2 –production process.
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Subadri, Intan, Adhi Satriyatama, and Ignatius D. M. Budi. "Facing Indonesia’s Future Energy with Bacterio-Algal Fuel Cells." Indonesian Journal of Energy 3, no. 2 (August 31, 2020): 68–82. http://dx.doi.org/10.33116/ije.v3i2.87.

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The energy crisis has become a global issue that has plagued almost all parts of the world. MFCs (Microbial Fuel Cells) is an alternative technology because of its ability to convert waste into electrical energy. The bacterio-algal fuel cell (BAFCs) is kind of an effort for increasing the economic value and carbon capture capability of MFCs. In this case, algae used as a catholyte and organic substrate containing anode-reducing exoelectrogenic bacteria acted as anolyte. This research will examine the potential of algae in BAFCs as an alternative energy for Indonesia's future. By photosynthesis reaction, bacterio-algal fuel cells are operated in a self-sustaining cycle. It can be configured in single, dual chambers, and triple chambers. The performance of bacterio-algal fuel cells is strongly influenced by the bacterial and algae species in each compartment. Factors involved in bacterial-algal fuel cells are also analyzed and assessed: electrode materials, membrane, carbon sources, and algae pretreatment, including the operational parameter, such as pH and temperature. Bacterio-algal fuel cells are recommended to be used to convert algae into electricity by scaling-up and integrating the devices. Organic substrate could be obtained from municipal wastewater. Algae as by-product could be harvested and converted into certain products. Algal Fuel Cell is the solution to produce electricity and reduce CO2 pollution at the same time. Also, an algal fuel cell is potential for sustainable use in the future. By integrating the algal fuel cell in the factory that produces high-concentrated wastewater, the fuel cell can purify the wastewater so that it is safe to be drained to the environment and also can make an integrated electricity production for the whole factory. Some ways to improve the power production are proposed to improve the power generation from BAFCs since this technology offers clean, affordable, sustainable energy, and in-line with SDGs.
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Abd El Baky, Hanaa H., and Gamal S. El Baroty. "Optimization of Hydrogen Gas Production Conditions from Egyptian Chlamydomonas Sp." Academic Journal of Life Sciences, no. 510 (October 15, 2019): 70–80. http://dx.doi.org/10.32861/ajls.510.70.80.

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Hydrogen gas could provide attractive options as ideal fuel for a world, in which environmental friendly and economically sustainable manner. Microalgae have the ability to bio-synthesis hydrogen gas. Algal H2 does do not generate any toxic or polluting bi-products and could potentially offer value-added products derived from algal biomass. In this work, the feasibility of coupling sulfur deprivation and light on hydrogen production by Chlamydomonas sp grown in photobioreactor was investigated. The cells growth, hydrogen production, total carbohydrate and chlorophyll content were determined. The results showed that, under optimum condition, algae cells were required 168 hr (7days) to reach the late logarithmic phase (the algal dry weight 4.11 g/L). Whereas the algae cells were needed about 18~22 days to reach this value (3.55 g/ L) when grow in optimum medium. The concentration of Chlorophyll (5.65%) and carbohydrate (39.46%) were accumulated in algae cells grow in S-deprives medium coupled with dark condition over that did in algae cells cultured in optimum medium. After about a 24 h of cultivation, photo-production of H2 was observed for C. sp either in absence or presence of sulfate. But under sulfur deprivation coupled with dark condition, higher H2 gas was obtained after 16 hr (7 several days) of incubation period. In new design photobioreactors (PhBRs), after 18 days of cultivation, the volume of H2 gas in was found to be 450 ml in cells grow in sulfur-deprived culture). This value was 360 ml in cells grow under optimal condition.
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Ban, Shidong, Weitie Lin, Fangyan Wu, and Jianfei Luo. "Algal-bacterial cooperation improves algal photolysis-mediated hydrogen production." Bioresource Technology 251 (March 2018): 350–57. http://dx.doi.org/10.1016/j.biortech.2017.12.072.

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21

Riley, Lesilie, Mark Dybdahl, and Robert Hall, Jr. "Grazing Effects of the New Zealand Mud Snail Across a Productivity Gradient in the Greater Yellowstone Ecosystem." UW National Parks Service Research Station Annual Reports 29 (January 1, 2005): 96–104. http://dx.doi.org/10.13001/uwnpsrc.2005.3623.

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Accurately predicting the effects of introduced species on native commumtles and ecosystems is a challenge. Utilizing methods of food web ecology, we measured grazing effects of the invasive freshwater New Zealand mud snail, Potamopyrgus antipodarum, in streams within the Greater Yellowstone Ecosystem. Previous results indicate that P. antipodarum can significantly reduce algal standing stocks in less than one week, but it is not yet known if grazing effects vary across streams differing in benthic algae production. In this study, we measured the strength of P. antipodarum grazing on algal resources across six streams varying widely in ambient primary production. In field enclosure experiments within each stream, we estimated direct grazing effects of snails on algae by measuring chlorophyll a, gross primary production and chlorophyll a-specific primary production. In most streams, P. antipodarum decreased overall algal standing stocks, as measured by chlorophyll a, even though gross primary production was not affected. As a result, chlorophyll-a specific primary production increased in productive streams. Finally, standardized comparisons of P. antipodarum-algae interactions indicated that grazing effects were largest in the most productive streams. The overall impact of P. antipodarum on native stream communities will be greatest in the most productive streams if these assemblages are also capable of supporting dense P. antipodarum populations.
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Powell, Ryan J., and Russell T. Hill. "Mechanism of Algal Aggregation by Bacillus sp. Strain RP1137." Applied and Environmental Microbiology 80, no. 13 (April 25, 2014): 4042–50. http://dx.doi.org/10.1128/aem.00887-14.

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ABSTRACTAlga-derived biofuels are one of the best alternatives for economically replacing liquid fossil fuels with a fungible renewable energy source. Production of fuel from algae is technically feasible but not yet economically viable. Harvest of dilute algal biomass from the surrounding water remains one of the largest barriers to economic production of algal biofuel. We identifiedBacillussp. strain RP1137 in a previous study and showed that this strain can rapidly aggregate several biofuel-producing algae in a pH- and divalent-cation-dependent manner. In this study, we further characterized the mechanism of algal aggregation by RP1137. We show that aggregation of both algae and bacteria is optimal in the exponential phase of growth and that the density of ionizable residues on the RP1137 cell surface changes with growth stage. Aggregation likely occurs via charge neutralization with calcium ions at the cell surface of both algae and bacteria. We show that charge neutralization occurs at least in part through binding of calcium to negatively charged teichoic acid residues. The addition of calcium also renders both algae and bacteria more able to bind to hydrophobic beads, suggesting that aggregation may occur through hydrophobic interactions. Knowledge of the aggregation mechanism may enable engineering of RP1137 to obtain more efficient algal harvesting.
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Puri, Munish. "Algal biotechnology for pursuing omega-3 fatty acid (bioactive) production." Microbiology Australia 38, no. 2 (2017): 85. http://dx.doi.org/10.1071/ma17036.

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Algae are spread in diversified ecosystems that include marine, freshwater, desert and hot springs and even snow and ice environments. Algae are classified as multicellular large sea weeds (macroalgae) or unicellular microalgae. Macroalgae are targeted for mining of natural biologically active components, which include proteins, linear peptides, cyclic peptides, and amino acids1. Recently, microalgae have been exploited for the production of high-value compounds such as lipids (omega-3 fatty acids), enzymes, polymers, toxins, antioxidants, and pigments (carotenoids)2. Thus, algal biotechnology is defined as ‘the technology developed using algae (macro or micro) to make or modify bioactive compounds, or products (nutritional supplements, fine chemicals) and renewable fuels for specific use’.
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Brahmbhatt*, Nayana Hasmukhbhai, R. T. Jasrai, and Rinku V. Patel. "Resoluteness of biogas potential of Cr, Pb & Cd accumulated green algae Spirogyra sp. and Oscillatoria sp." International Journal of Bioassays 6, no. 01 (December 31, 2016): 5224. http://dx.doi.org/10.21746/ijbio.2017.01.008.

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Water pollution due to heavy metals are spreading world-wide along with industrial progress. In this research work cultivation of Spirogyra sp. & Oscillatoria sp. were done and wastewater treatment of heavy metal such as Cr, Cd & Pb was accumulated by algae. After treatment of algae Spirogyra sp. & Oscillatoria sp. were decomposed and to prepare compost. The production of biogas from algal biomass becomes economically feasible and cheaper. This present study revealed that the nutrient content of natural compost was recorded about C- 38.5%, N- 3.31%, P- 1.02%, & K- 3.42%, heavy metal content of Cd- BDL, Pb- 0.21ml/mg & Cr- 0.01ml/mg and biogas value 19.19 l/kg. The maximum value of biogas was recorded at 30 ppm concentration (27.95 l/kg) of algal compost as compare to natural compost (19.19 l/kg) in Spirogyra sp. The experimental study concluded that the selected algae showed better biogas production as compared to natural. Whereas out of selected two algae, Spirogyra sp. gave better outcome as compare to Oscillatoria sp. Thus, Study suggested that algal biomass constitute a promising, efficient, cheap, decayed material for making compost and biogas production.
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STEWART, F. J., and C. H. FRITSEN. "Bacteria-algae relationships in Antarctic sea ice." Antarctic Science 16, no. 2 (June 2004): 143–56. http://dx.doi.org/10.1017/s0954102004001889.

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Energy transfer in microbial food webs is partly quantified by the relationship between bacterial and algal biomass. Tight spatial relationships suggest active bacterial assimilation of dissolved photosynthate in temperate marine and fresh waters. However, studies in the Antarctic suggest that bacterial biomass generation from algal-derived dissolved organic matter is highly variable across seasons and habitats. Regression analysis was used to measure how bacteria covaried with algae in sea ice and water column habitats at three sites around Antarctica. Bacteria and algae were positively related in sea ice of the Weddell Sea during early winter 1992 (r2 = 0.16, slope = 0.24) and across sea ice and upper water column habitats of the Ross Sea during summer 1999 (r2 = 0.52, slope = 0.50). Conversely, bacteria and algae exhibited no discernible relationship in the water column and first year ice habitats of the Western Antarctic Peninsula region in winter 2001 (r2 = 0.003, slope = −0.04). Low algal production and residual biomass probably limited bacterial production and facilitated bacteria-algae uncoupling in winter sea ice of the Western Antarctic Peninsula. Winter sea ice algal biomass was probably limited by a relatively late date of initial ice formation, reduced multi-year ice coverage, and a lack of radiant energy in the winter ice pack.
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26

Derabe Maobe, H., M. Onodera, M. Takahashi, H. Satoh, and T. Fukazawa. "Control of algal production in a high rate algal pond: investigation through batch and continuous experiments." Water Science and Technology 69, no. 12 (April 8, 2014): 2519–25. http://dx.doi.org/10.2166/wst.2014.174.

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For decades, arid and semi-arid regions in Africa have faced issues related to water availability for drinking, irrigation and livestock purposes. To tackle these issues, a laboratory scale greywater treatment system based on high rate algal pond (HRAP) technology was investigated in order to guide the operation of the pilot plant implemented in the 2iE campus in Ouagadougou (Burkina Faso). Because of the high suspended solids concentration generally found in effluents of this system, the aim of this study is to improve the performance of HRAPs in term of algal productivity and removal. To determine the selection mechanism of self-flocculated algae, three sets of sequencing batch reactors (SBRs) and three sets of continuous flow reactors (CFRs) were operated. Despite operation with the same solids retention time and the similarity of the algal growth rate found in these reactors, the algal productivity was higher in the SBRs owing to the short hydraulic retention time of 10 days in these reactors. By using a volume of CFR with twice the volume of our experimental CFRs, the algal concentration can be controlled during operation under similar physical conditions in both reactors.
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Phillips, Enosh. "Algal nanotech for biofuel production." Agrica 9, no. 1 (2020): 1. http://dx.doi.org/10.5958/2394-448x.2020.00003.6.

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28

Urooj, Shabana, Athar Hussain, and Narayani Srivastava. "Biodiesel Production from Algal Blooms." International Journal of Measurement Technologies and Instrumentation Engineering 2, no. 3 (July 2012): 60–71. http://dx.doi.org/10.4018/ijmtie.2012070106.

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Usage of Bio-energy is becoming more and more prominent due to the peak oil crisis. Bio-energy is the energy which can be synthesized using methods and raw material which are available in nature and are derived from the biological sources. They are referred as bio-mass energy, bio-diesel, and bio-power. In this paper the study has been carried out on bio-energy generation in form of bio-diesel and the bio-diesel is produced in the laboratory conditions by using base catalyzed trans-esterification process. The nomenclature bio-diesel is given to the oil which can be generated by using the raw materials which are renewable and are waste materials. It doesn’t contain any percentage of petroleum products in it. It is called bio-diesel because it can be further used to run the diesel engine. In this paper biodiesel is generated using local pond algae by the process of base catalyzed trans-esterification.
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Bird, Michael I., Christopher M. Wurster, Pedro H. de Paula Silva, Adrian M. Bass, and Rocky de Nys. "Algal biochar – production and properties." Bioresource Technology 102, no. 2 (January 2011): 1886–91. http://dx.doi.org/10.1016/j.biortech.2010.07.106.

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30

Weyer, Kristina M., Daniel R. Bush, Al Darzins, and Bryan D. Willson. "Theoretical Maximum Algal Oil Production." BioEnergy Research 3, no. 2 (October 8, 2009): 204–13. http://dx.doi.org/10.1007/s12155-009-9046-x.

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31

Hassaan, Mohamed A., Ahmed El Nemr, Marwa R. Elkatory, Ahmed Eleryan, Safaa Ragab, Amany El Sikaily, and Antonio Pantaleo. "Enhancement of Biogas Production from Macroalgae Ulva latuca via Ozonation Pretreatment." Energies 14, no. 6 (March 18, 2021): 1703. http://dx.doi.org/10.3390/en14061703.

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One of the dominant species of green algae growing along the Mediterranean coast of Egypt is Ulva lactuca. Pretreatment can have a major effect on biogas production because hydrolysis of the algae cell wall structure is a rate-limiting stage in the anaerobic digestion (AD) process. The use of ozone, a new pretreatment, to boost biogas production from the green algae Ulva lactuca was investigated in this study. Ozonation at various dosages was used in contrast to untreated biomass, and the effect on the performance of subsequent mesophilic AD using two separate inoculums (cow manure and activated sludge) was examined. The findings indicated that, in different studies, ozonation pretreatment showed a substantial increase in biogas yield relative to untreated algae. With an ozone dose of 249 mg O3 g–1 VS algal for Ulva lactuca, the highest biogas output (498.75 mL/g VS) was achieved using cow manure inoculum. The evaluation of FTIR, TGA, SEM, and XRD revealed the impact of O3 on the structure of the algal cell wall and integrity breakage, which was thus established as the main contributor to improving the biogas production.
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32

Vasileva, Ivanina, Juliana Ivanova, and Svetoslav Alexandrov. "Bioethical considerations for algal biotechnology." Bangladesh Journal of Bioethics 9, no. 2 (April 25, 2019): 1–5. http://dx.doi.org/10.3329/bioethics.v9i2.41184.

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In this manuscript we discuss different aspects and applications of algal biotechnology and how they are seen through the prism of bioethics. We review how algae have been considered to solve problems on Earth and to ease human suffering. We also take a look at the current state of the production of algal biomass and we offer our suggestions and considerations based on the fact that the biomass is an expensive product and yet its quality is very good.
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33

Rose, P. D., B. A. Maart, T. D. Phillips, S. L. Tucker, A. K. Cowan, and R. A. Rowswell. "Cross-Flow Ultrafiltration Used in Algal High Rate Oxidation Pond Treatment of Saline Organic Effluents with the Recovery of Products of Value." Water Science and Technology 25, no. 10 (May 1, 1992): 319–27. http://dx.doi.org/10.2166/wst.1992.0258.

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An algal high rate oxidation ponding process for treating organic s present in saline effluents has been described. The extreme halophile Dunaliella salina can be made to predominate in the system by manipulating salinity, producing products of value together with a waste treatment function. Application in treating tannery saline organic wastes was examined. Techniques appropriate for the harvesting of micro-algae from this and other algal production systems presents a limiting factor in the development of algal biotechnology. Cross-flow filtration was evaluated as a technique for micro-algal cell separation. Both microfiltration and ultrafiltration were found to produce effective algal removal from the medium, Cross-flow ultrafiltration with a polyethersulfone coated tubular filter produced effective separation with the production of cell concentrates in a viable condition. Flux rates of 30 - 40 LMH fall within acceptable levels for application in industrial processes. Cell shattering observed with microfiltration precludes its use for recovering whole or viable cell concentrates.
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34

Lakatos, Gergely, Daniella Balogh, Attila Farkas, Vince Ördög, Péter Tamás Nagy, Tibor Bíró, and Gergely Maróti. "Factors influencing algal photobiohydrogen production in algal-bacterial co-cultures." Algal Research 28 (December 2017): 161–71. http://dx.doi.org/10.1016/j.algal.2017.10.024.

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35

Tiwari, Vinaya, Alok Das, Shallu Thakur, and Rakesh Kumar Trivedi. "Selection of Indigenous Algal Species for Potential Biodiesel Production." Journal of Pure and Applied Microbiology 15, no. 2 (June 1, 2021): 851–63. http://dx.doi.org/10.22207/jpam.15.2.40.

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Currently, India utilizes an enormous amount of fossil fuels and a major quantity of fossil fuels are imported from other countries. It’s a giant load on the Indian Economy. The burning of fossil fuels causes global warming. Carbon neutral, renewable fuels are essential for environmental protection and it’s economically sustainable for India. Biofuels attention day by day due to a rise in energy demands and environmental concerns. Biodiesel produced from algal oil a possible renewable and carbon-neutral substitute to fossil fuels. The feasibility of the algal-based biodiesel industry depends on the selection of adequate species regarding commercial oil yields and oil quality. Present research work to bioprospecting and screening of 19 algal and blue-green algal species, the oil percentage and the fatty acid profiles, used for analyzing the biodiesel fuel properties. Oil from Tolypothrix phyllophila algal strain and compared it with another eighteen algal and blue-green algal strains from different literature. Tolypothrix phyllophila algal strain contains approximately 12.6% lipid on a dry weight basis. We also compared the FAME profile of 19 algal and blue-green algal strains and calculated and compared the fuel properties such as cetane number, Iodine Value, etc. of the biodiesel derived from these algal and blue-green algal oils based on chain length and saturation. We also investigated the 19 algal and blue-green algal fatty acid profiles and its suitability for biodiesel production and strains selection through PROMETHEE (Preference Ranking Organization Method for Enrichment Evaluations) and GAIA (geometrical analysis for interactive aid) analysis.
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36

Molinari, Bianca, Ben Stewart-Koster, Tim J. Malthus, and Stuart E. Bunn. "Assessing Spatial Variation in Algal Productivity in a Tropical River Floodplain Using Satellite Remote Sensing." Remote Sensing 13, no. 9 (April 28, 2021): 1710. http://dx.doi.org/10.3390/rs13091710.

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Studies of tropical floodplains have shown that algae are the primary source material for higher consumers in freshwater aquatic habitats. Thus, methods that can predict the spatial variation of algal productivity provide an important input to better inform management and conservation of floodplains. In this study, a prediction of the spatial variability in algal productivity was made for the Mitchell River floodplain in northern Australia. The spatial variation of aquatic habitat types and turbidity were estimated using satellite remote sensing and then combined with statistical modelling to map the spatial variation in algal primary productivity. Open water and submerged plants habitats, covering 79% of the freshwater flooded floodplain extent, had higher rates of algal production compared to the 21% cover of emergent and floating aquatic plant habitats. Across the floodplain, the predicted average algal productivity was 150.9 ± 95.47 SD mg C m−2 d−1 and the total daily algal production was estimated to be 85.02 ± 0.07 SD ton C. This study provides a spatially explicit representation of habitat types, turbidity, and algal productivity on a tropical floodplain and presents an approach to map ‘hotspots’ of algal production and provide key insights into the functioning of complex floodplain–river ecosystems. As this approach uses satellite remotely sensed data, it can be applied in different floodplains worldwide to identify areas of high ecological value that may be sensitive to development and be used by decision makers and river managers to protect these important ecological assets.
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37

Tedesco, L., M. Vichi, and E. Scoccimarro. "Sea-ice algal phenology in a warmer Arctic." Science Advances 5, no. 5 (May 2019): eaav4830. http://dx.doi.org/10.1126/sciadv.aav4830.

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The Arctic sea-ice decline is among the most emblematic manifestations of climate change and is occurring before we understand its ecological consequences. We investigated future changes in algal productivity combining a biogeochemical model for sympagic algae with sea-ice drivers from an ensemble of 18 CMIP5 climate models. Model projections indicate quasi-linear physical changes along latitudes but markedly nonlinear response of sympagic algae, with distinct latitudinal patterns. While snow cover thinning explains the advancement of algal blooms below 66°N, narrowing of the biological time windows yields small changes in the 66°N to 74°N band, and shifting of the ice seasons toward more favorable photoperiods drives the increase in algal production above 74°N. These diverse latitudinal responses indicate that the impact of declining sea ice on Arctic sympagic production is both large and complex, with consequent trophic and phenological cascades expected in the rest of the food web.
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38

Luxem, Katja E., Bas Vriens, Renata Behra, and Lenny H. E. Winkel. "Studying selenium and sulfur volatilisation by marine algae Emiliania huxleyi and Thalassiosira oceanica in culture." Environmental Chemistry 14, no. 4 (2017): 199. http://dx.doi.org/10.1071/en16184.

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Environmental contextVolatile selenium compounds from the oceans may ultimately be an important selenium source for agricultural soils. It has been hypothesised that marine algae are responsible for volatile selenium emissions, but in laboratory experiments, we observed minimal volatile selenium production by two marine algae known to produce large amounts of volatile sulfur. Instead, we found hints that bacterial processes may be important in the production of volatile selenium in the oceans. AbstractVolatile methylated selenium compounds, especially dimethylselenide, are thought to comprise the majority of marine selenium emissions. Despite their potential importance for the global redistribution of this trace element, which is essential for human health, little is known about the algal production of volatile organic selenium compounds. Previous studies have found correlations between dissolved dimethylselenide concentrations, dimethylsulfide concentrations (the sulfur analogue of dimethylselenide) and proxies for algal activity, most notably during a bloom of the coccolithophorid Emiliania huxleyi. In culturing studies, we investigated the ability of three globally important marine algal species, E. huxleyi, Phaeocystis globosa and the diatom Thalassiosira oceanica, to produce dimethylselenide. Despite substantial uptake of selenium and the production of volatile sulfur, E. huxleyi and T. oceanica produced negligible volatile selenium (<2nM). P. globosa produced low amounts of volatile selenium (~8nM), but grew poorly in our laboratory. However, cultures of marine bacteria and mixed bacterial–algal cultures showed that substantial amounts of volatile selenium can be produced in the presence of marine bacteria. In addition, a culture of marine bacteria alone produced ~50nM volatile selenium, far more than axenic cultures of E. huxleyi when exposed to equivalent selenite concentrations. Our results hint that marine algae may be of minor importance in the direct production of volatile selenium in the oceans, and suggest that the production of these compounds in the marine biosphere may instead be controlled by bacterial activity.
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Michell, Catherine, Marc Lahaye, Christian Bonnet, Serge Mabeau1, and Jean-Luc Barry. "In vitrofermentation by human faecal bacteria of total and purified dietary fibres from brown seaweeds." British Journal of Nutrition 75, no. 2 (February 1996): 263–80. http://dx.doi.org/10.1017/bjn19960129.

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AbstractThe in vitro degradation of dietary fibre from three brown seaweeds (Himanthalia elongata, Laminaria digitata and Undaria pinnatiJda) was studied, using human faecal flora. Two sets of fibre were tested: (1) total algal fibres extracted from the whole algae, mainly composed of alginates, and (2) purified fibres (sulphated fucans, Na-alginates and laminarans) representative of those contained in the whole brown algae. Mannuronate, one algal component, was also investigated. Substrate disappearance and short- chain fatty acid (SCFA) production were monitored after 6, 12 and 24 h fermentation. Gas production was followed hourly during the first 9 h and then at 12 and 24 h. Sugarbeet fibre was used as a fermentation reference substrate. According to the fermentative indices used, most of each of the total algal fibres disappeared after 24 h (range 60–76 %) hut, unlike the reference substrate, they were not completely metabolized to SCFA (range 47–62 %). Among the purified algal fibres, disappearance of laminarans was approximately 90% and metabolism to SCFA was approximately 85% in close agreement with the fermentation pattern of reference fibres. Sulphated fucans were not degraded. Na- alginates exhibited a fermentation pattern quite similar to those of the whole algal fibres with a more pronounced discrepancy between disappearance and production of SCFA: disappearance was approximately 83 % but metabolism was only approximately 57 YO. Mannuronate was slowly fermented hut its metabolism corresponded to its disappearance from the fermentative medium. Thus, the characteristic fermentation pattern of the total fibres from the three brown algae investigated was attributed to the peculiar fermentation of alginates, and mannuronate was shown not to be directly involved.
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40

A, Aarthy, Smita Kumari, Prachi Turkar, and Sangeetha Subramanian. "AN INSIGHT ON ALGAL CELL DISRUPTION FOR BIODIESEL PRODUCTION." Asian Journal of Pharmaceutical and Clinical Research 11, no. 2 (February 1, 2018): 21. http://dx.doi.org/10.22159/ajpcr.2018.v11i2.22481.

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Objective: This review article deals with the effect that various cell disruption techniques have on the efficiency of lipid extraction. We have reviewed existing algal cell disruption techniques that aid the biodiesel production process.Methods: Current rise in demand for energy has led the researcher to focus on the production of sustainable fuels, among which biodiesel has received greater attention. This is due to its larger lipid content, higher growth rate, larger biomass production, and lower land use. Extraction of lipid from algae (micro and macro) for the production of biodiesel involves numerous downstream processing steps, of which cell wall disruption is a crucial step. Bead milling, high-pressure homogenization, ultra-sonication, freeze-drying, acid treatment, and enzymatic lysis are some methods of cell disruption. The cell disruption technique needs to be optimized based on the structure and biochemical composition of algae.Result: The lipid extraction efficiency varies depending on the algal species and the cell disruption technique used.Conclusion: In-depth research and development of new techniques are required to further enhance the cell disruption of the algal cell wall for the enhanced recovery of lipids. In addition, the operating costs and energy consumption should also be optimized for the cost-effective recovery.
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41

Smith, Daniel Wilkins. "Biological Control of Excessive Phytoplankton Growth and the Enhancement of Aquacultural Production." Canadian Journal of Fisheries and Aquatic Sciences 42, no. 12 (December 1, 1985): 1940–45. http://dx.doi.org/10.1139/f85-240.

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A method is proposed to control phytoplankton biomass in aquacultural ponds, using both zooplankton and filter-feeding silver carp (Hypophthalmichthys molitrix). The technique maintains co-existence of zooplankton and filter-feeding fish by excluding the fish from part of the water column. Zooplankton, which feed on smaller algal species, and silver carp, which feed on large algae and zooplankton, together can consume all sizes of phytoplankton, thus controlling algal biomass. This technique was tested in 1000-L tanks, some containing channel catfish (Ictalurus punctatus) alone, some both catfish and silver carp, and others catfish and silver carp with a zooplankton refuge. The refuge permitted coexistence of high densities of large zooplankters with the filter-feeding fish. This combination of filter-feeders reduced algal biomass by as much as 99%, increased phytoplankton diversity, and showed a trend toward improved silver carp growth compared with treatments without a refuge. The proposed technique could be applied to both intensive and extensive aquacultural systems.
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42

Fisher, Carolyn L., Pamela D. Lane, Marion Russell, Randy Maddalena, and Todd W. Lane. "Low Molecular Weight Volatile Organic Compounds Indicate Grazing by the Marine Rotifer Brachionus plicatilis on the Microalgae Microchloropsis salina." Metabolites 10, no. 9 (September 4, 2020): 361. http://dx.doi.org/10.3390/metabo10090361.

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Microalgae produce specific chemicals indicative of stress and/or death. The aim of this study was to perform non-destructive monitoring of algal culture systems, in the presence and absence of grazers, to identify potential biomarkers of incipient pond crashes. Here, we report ten volatile organic compounds (VOCs) that are robustly generated by the marine alga, Microchloropsis salina, in the presence and/or absence of the marine grazer, Brachionus plicatilis. We cultured M. salina with and without B. plicatilis and collected in situ volatile headspace samples using thermal desorption tubes over the course of several days. Data from four experiments were aggregated, deconvoluted, and chromatographically aligned to determine VOCs with tentative identifications made via mass spectral library matching. VOCs generated by algae in the presence of actively grazing rotifers were confirmed via pure analytical standards to be pentane, 3-pentanone, 3-methylhexane, and 2-methylfuran. Six other VOCs were less specifically associated with grazing but were still commonly observed between the four replicate experiments. Through this work, we identified four biomarkers of rotifer grazing that indicate algal stress/death. This will aid machine learning algorithms to chemically define and diagnose algal mass production cultures and save algae cultures from imminent crash to make biofuel an alternative energy possibility.
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43

Culaba, Alvin B., Aristotle T. Ubando, Phoebe Mae L. Ching, Wei-Hsin Chen, and Jo-Shu Chang. "Biofuel from Microalgae: Sustainable Pathways." Sustainability 12, no. 19 (September 28, 2020): 8009. http://dx.doi.org/10.3390/su12198009.

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As the demand for biofuels increases globally, microalgae offer a viable biomass feedstock to produce biofuel. With abundant sources of biomass in rural communities, these materials could be converted to biodiesel. Efforts are being done in order to pursue commercialization. However, its main usage is for other applications such as pharmaceutical, nutraceutical, and aquaculture, which has a high return of investment. In the last 5 decades of algal research, cultivation to genetically engineered algae have been pursued in order to push algal biofuel commercialization. This will be beneficial to society, especially if coupled with a good government policy of algal biofuels and other by-products. Algal technology is a disruptive but complementary technology that will provide sustainability with regard to the world’s current issues. Commercialization of algal fuel is still a bottleneck and a challenge. Having a large production is technical feasible, but it is not economical as of now. Efforts for the cultivation and production of bio-oil are still ongoing and will continue to develop over time. The life cycle assessment methodology allows for a sustainable evaluation of the production of microalgae biomass to biodiesel.
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Mateescu, C., A. Dima, and D. Marin. "Sustainable solution for alien algae management to reduce the environmental consequences of sea and river transport." Technium: Romanian Journal of Applied Sciences and Technology 2, no. 1 (January 16, 2020): 97–103. http://dx.doi.org/10.47577/technium.v2i1.67.

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The accelerated development of sea and river transport over the past few decades has led to a negative phenomenon regarding the invasion and development of alien algal species, threating to modify the ecosystem functions. Algae are an attractive feedstock for biofuels production due to many advantages such as rapid growth, chemical composition rich in lipids, carbohydrates and proteins, but also because algae do not require farmland and do not create controversy in using arable land for energy purpose to the detriment of food-intended crops. Indigenous or alien algal biomass has been excessively developed in the Black Sea basin and areas affected by eutrophication, causing environmental problems. This paper presents a sustainable management solution for mitigating the negative impact of alien algae by exploiting their energy potential for producing biogas. An innovative concept of tubular bioreactor for biogas production was developed within INCDIE ICPE-CA and could be successfully applied to harness algal biomass and produce biogas for domestic and industrial use, while simultaneously ensuring sanitation of sea shore and fresh water streams.
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Yadala, Soumya, Justin D. Smith, David Young, Daniel W. Crunkleton, and Selen Cremaschi. "Optimization of the Algal Biomass to Biodiesel Supply Chain: Case Studies of the State of Oklahoma and the United States." Processes 8, no. 4 (April 18, 2020): 476. http://dx.doi.org/10.3390/pr8040476.

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The goal of this work is to design a supply chain network that distributes algae biomass from supply locations to meet biodiesel demand at specified demand locations, given a specified algae species, cultivation (i.e., supply) locations, demand locations, and demand requirements. The final supply chain topology includes the optimum sites to grow biomass, to extract algal oil from the biomass, and to convert the algae oil into biodiesel. The objective is to minimize the overall cost of the supply chain, which includes production, operation, and transportation costs over a planning horizon of ten years. Algae production was modeled both within the U.S. State of Oklahoma, as well as the entire contiguous United States. The biodiesel production cost was estimated at $7.07 per U.S. gallon ($1.87 per liter) for the State of Oklahoma case. For the contiguous United States case, a lower bound on costs of $13.68 per U.S. gallon ($3.62 per liter) and an upper bound of $61.69 ($16.32 per liter) were calculated, depending on the transportation distance of algal biomass from production locations.
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Naik, Aishwarya N., Mrinalini Singh, and Yasrib Qurishi. "Algal biofuel: A promising perspective." Annals of Plant Sciences 7, no. 5 (April 30, 2018): 2262. http://dx.doi.org/10.21746/aps.2018.7.5.10.

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The depleting energy resources and rising environmental issues have led to significant research in the field of producing fuel using alternative means. Biofuel can serve as better means to cope up with the depleting fossil and petroleum fuels. The novel properties of algae have set them as the best among all other biomasses and as a better alternative to the energy crisis. Algal biofuels are grouped under “Third generation biofuels” which has gained significant attention recently. Combustion of fossil and petroleum fuel releases sulphur dioxide in the air causing air pollution and acid rain. Most of the research on algal biofuel is done using microalgae which have high oil content along with faster growth rate. The potential of algae for producing biofuel can be improved by obtaining more efficient methods and by overcoming its certain limitations. The present review highlights the advantages, various types and production of algal biofuel.
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47

Liu, Jun Zhi, Ya Ming Ge, and Guang Ming Tian. "Enhancement of Hydrocarbon Productivity of Botryococcus braunii by an Adenine Type Phytohormone." Advanced Materials Research 512-515 (May 2012): 397–400. http://dx.doi.org/10.4028/www.scientific.net/amr.512-515.397.

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This study examined the effects of an adenine-type cytokinin 6-benzylaminopurine (6-BA) on the growth and metabolism characteristics of Botryococcus braunii, one of the most promising oil-rich algae for biofuel production. The results showed that 6-BA of low dose (0.1-1.0 mg L-1) would enhance the algal growth rate and biochemical synthesis, whereas too much (5.0 mg L-1) would be lethally toxic for B. braunii. Noticingly, though the maximum algal growth rate, chlorophyll and β-carotenoid content were observed in the treatment with 0.5 and/or 1.0 mg L-1 6-BA, both the maximum algal hydrocarbon content and the highest hydrocarbon productivity were observed in the treatment with 0.1 mg L-1 6-BA, which were respectively 2.45 and 3.48 times of the control (39.1% vs. 16.0%, 546 mg L-1 vs. 157 mg L-1). This finding has great implications for improving algae biofuels production by phytohormone.
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48

Neethu, B., H. Pradhan, Pankaj Sarkar, and M. M. Ghangrekar. "Application of ion exchange membranes in enhancing algal production alongside desalination of saline water in microbial fuel cell." MRS Advances 4, no. 19 (2019): 1077–85. http://dx.doi.org/10.1557/adv.2019.170.

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AbstractIn the present world scenario the demand for fresh water and clean energy is driving the need to convert a microbial fuel cell (MFC) into an algal-based microbial desalination cell (MDC) that can support algal growth along with desalination of saline water. In this study, the performance of a five-chambered MDC fed with saline water having two different salt concentrations, namely 2.5 g/L and 5.0 g/L in desalination chamber, as well as MDC operated without algae in catholyte was investigated. The algal-based MDC operated with 5 g/L of total dissolved solid (TDS) in desalination chamber exhibited the best performance results among all other combinations giving a maximum power density of 45.52 mW/m2 and a desalination efficiency of 71 ± 2 %. Also, a chemical oxygen demand (COD) removal efficiency of 78 % and coulombic efficiency of 12.24 % was achieved with 5 g/L NaCl concentration in desalination chamber. Based on this experimental performance evaluation, it can be inferred that algal-based MDC can provide a promising and sustainable approach for wastewater treatment with the capability of simultaneous desalination, algal production and electrical energy recovery.
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49

Trentacoste, Emily M., Alice M. Martinez, and Tim Zenk. "The place of algae in agriculture: policies for algal biomass production." Photosynthesis Research 123, no. 3 (March 6, 2014): 305–15. http://dx.doi.org/10.1007/s11120-014-9985-8.

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50

Ainuri, Muhammad Z., Hermin P. Kusumaningrum, and Endang Kusdiyantini. "Microbiological and Ecophysiological Characterization of Green Algae Dunaliella sp. for Improvement of Carotenoid Production." Jurnal Natur Indonesia 10, no. 2 (November 20, 2012): 66. http://dx.doi.org/10.31258/jnat.10.2.66-69.

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An isolate of green algae Dunaliella sp. from BBAP Jepara is usually used as a source for carotenoid supplementfor marine animal cultivation in the local area. In order to improve carotenoid production especially detection ofbiosynthetic pathway from the organisms investigated in this study, the main purpose of this study is characterizingDunaliella sp. based on it’s microbiological and ecophysiological characters. The research was done by characterizethe growth, the cell and colonies microbiologically, total pigment production, and also characterize all of theecophysiological factors affecting the algal growth and survival. The results of this research showed that Dunaliellasp. posseses typical characteristic of green eucaryote alga, in their growth and ecological condition. The extremecharacters which was toleration ability to high salinity environment of was used to conclude Dunaliella sp. asDunaliella salina.
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