Academic literature on the topic 'Alizarin Red S Staining'

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Journal articles on the topic "Alizarin Red S Staining"

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Caraguel, Jean-Marie, Thomas Barreau, Sarah Brown-Vuillemin, and Samuel P. Iglésias. "In vivo staining with alizarin for ageing studies on chondrichthyan fishes." Aquatic Living Resources 33 (2020): 1. http://dx.doi.org/10.1051/alr/2020002.

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Age determination for stock assessments and conservation of cartilaginous fishes is mainly obtained by counting the annual growth bands in vertebrae. Recent studies show numerous inconsistencies and the need for systematic validation. We assessed the effectiveness of the fluorochrome alizarin red S, a common skeleton vital marker used as a time stamp for teleost fishes, on chondrichthyan. Twenty-five captive small-spotted catsharks (Scyliorhinus canicula) were marked by alizarin red S intraperitoneal injections. The fluorochrome produced a wide fluorescent mark on sectioned vertebral centra of
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Luthfi, Muhammad Ja’far. "Modified Alizarin Red S-Alcian Blue Staining for Reptilian Skeleton." Biology, Medicine, & Natural Product Chemistry 5, no. 1 (2016): 19. http://dx.doi.org/10.14421/biomedich.2016.51.19-22.

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<p>Skeletal staining is an important method in anatomical study. The aim of the research was to develop staining and clearing method of Reptilian skeleton using Alizarin Red S-Alcian Blue. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. This modified double-staining has successfully stain bone and cartilage of Reptilian.</p>
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White, Kristopher, Rabab Chalaby, Gina Lowe, Jacob Berlin, Carlotta Glackin, and Ronke Olabisi. "Calcein Binding to Assess Mineralization in Hydrogel Microspheres." Polymers 13, no. 14 (2021): 2274. http://dx.doi.org/10.3390/polym13142274.

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We describe a method to assess mineralization by osteoblasts within microspheres using calcein. Fluorescence imaging of calcein bound to the calcium in hydroxyapatite permits assessment of the mineralized portion of the extracellular matrix. Colorimetric imaging of Alizarin Red S complexed with calcium also gives measures of mineralization, and in tissue cultures calcein and Alizarin Red S have been shown to bind to the same regions of mineral deposits. We show that when the mineralization takes place within hydrogel microspheres, Alizarin Red S does not stain mineral deposits as consistently
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Bernar, Aline, Jennifer Viktoria Gebetsberger, Monika Bauer, Werner Streif, and Michael Schirmer. "Optimization of the Alizarin Red S Assay by Enhancing Mineralization of Osteoblasts." International Journal of Molecular Sciences 24, no. 1 (2022): 723. http://dx.doi.org/10.3390/ijms24010723.

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The alizarin red S assay is considered the gold standard for quantification of osteoblast mineralization and is thus widely used among scientists. However, there are several restrictions to this method, e.g., moderate sensitivity makes it difficult to uncover slight but significant effects of potentially clinically relevant substances. Therefore, an adaptation of the staining method is appropriate and might be obtained by increasing the mineralization ability of osteoblasts. In this study, cell culture experiments with human (SaOs-2) and murine (MC3T3-E1) osteoblasts were performed under the a
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Afshar, M., N. Taibi, and M. J. Golalipoor. "Tu-P7:286 Modified alizarin red S staining to detect vascular calcification." Atherosclerosis Supplements 7, no. 3 (2006): 247–48. http://dx.doi.org/10.1016/s1567-5688(06)80989-x.

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Mori, Fumiaki, and Goyo Koya. "CEREBELLAR CALCIFICATION DETECTED BY ALIZARIN RED S STAINING IN THIOPHENE-EXPOSED RATS." Journal of Toxicologic Pathology 8, no. 4 (1995): 391–99. http://dx.doi.org/10.1293/tox.8.391.

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Rakhmiyati, Rakhmiyati, and Muhammad Ja’far Luthfi. "Alizarin Red S-Alcian Blue Staining for Regenerated tail of Common House Gecko (Hemidactylus frenatus)." Biology, Medicine, & Natural Product Chemistry 7, no. 2 (2018): 57–59. http://dx.doi.org/10.14421/biomedich.2018.72.57-59.

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Common House Gecko (Hemidactylus frenatus) is one of reptiles that have ability to autotomy their tails. Tail autotomy is a mechanism to protect it self from predators. After the tail broke, there will be wound healing on the tail which is then followed by a tail regeneration event. Original tail and regenerate tail is very different morphologically and anatomically. The original tail is composed of bones while the tail of the regenerate is composed of cartilage. Histochemical staining using Alizarin Red-S Alcian Blue was done to differentiate bone and cartilage. This method will stained bones
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Kim, Da-Sol, Soo-Kyung Bae, Yeon-Ju Kwak, Geum-Joung Youn, and Hye-Ock Jang. "Osteogenic Potential of Osteolforte: Gene and Protein-Level Evaluation in Human Bone Marrow Stromal Cells." Current Issues in Molecular Biology 47, no. 8 (2025): 588. https://doi.org/10.3390/cimb47080588.

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Osteolforte, a compound with potential bone-regenerative properties, was investigated for its effects on human bone marrow stromal cells (hBMSCs). This study aimed to evaluate its impact on cell viability, osteogenic differentiation, and both gene and protein expression using a combination of assays, including CCK-8, Alizarin Red S staining, Quantitative Real-Time PCR (qRT-PCR), and Western blot analysis. The results demonstrated that Osteolforte significantly enhanced osteogenic differentiation in hBMSCs. Alizarin Red S staining revealed increased mineralization, indicating elevated calcium d
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BUI, E. N., and A. R. MERMUT. "QUANTIFICATION OF SOIL CALCIUM CARBONATES BY STAINING AND IMAGE ANALYSIS." Canadian Journal of Soil Science 69, no. 3 (1989): 677–82. http://dx.doi.org/10.4141/cjss89-066.

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Quantification of calcium carbonates in a light-colored matrix or disseminated calcium carbonates cannot be obtained directly from impregnated blocks by image analysis. A method that combined staining by alizarin red-S with digital image analysis made it possible to identify and quantify soil calcium carbonates including the disseminated form in thin sections. Best results were obtained under reflected light using a tungsten lamp source. Photographic filters did not improve the image quality of stained calcium carbonates. Key words: Image analysis, soil micromorphometry
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Luthfi, Muhammad Ja’far, and Nyoman Puniawati Soesilo. "A Simple Method for Clearing and Staining Specimens for The Demonstration of Animal Skeleton." Biology, Medicine, & Natural Product Chemistry 8, no. 1 (2019): 17–21. http://dx.doi.org/10.14421/biomedich.2019.81.17-21.

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Teaching skeletal system would be more attractive using real/preserved specimen compared to those using only book. The aim of the research was to develop clearing and staining method of animal skeleton specimen using Alizarin Red S-Alcian Blue as teaching aid tool. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. The spesimen will increase effectiveness in elementary school science teaching and learning.
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Dissertations / Theses on the topic "Alizarin Red S Staining"

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Guimarães, Celeste Blumenthal. "Avaliação do endotélio corneano de galinhas (Gallus gallus domesticus) após exposição ao besilato de atracúrio – estudo in vitro." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2015. http://hdl.handle.net/10183/122437.

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A musculatura iridiana de várias espécies de aves é estriada esquelética, dessa forma o controle da midríase transoperatória não é conseguida por agentes que atuem no sistema nervoso autônomo, como ocorre nos mamíferos. O besilato de atracúrio é um agente neurobloqueador que atua no miorrelaxamento esquelético. O uso intracameral deste fármaco tem sido realizado em aves e promove midríase imediata, a qual é fundamental em cirurgias para remoção da catarata. Objetivou-se avaliar a repercussão do uso de besilato de atracúrio no endotélio da córnea de galinhas. Utilizaram-se 20 córneas de 10 gali
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Bhat, Samerna. "Impact of Nicotine and PPARd-agonist on Human Mesenchymal Stem Cells." University of Toledo Health Science Campus / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=mco1366337315.

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Moreira, Eduardo Marques. "Tratamento eletroquímico e eletroquímico irradiado do corante vermelho de alizarina S." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/75/75131/tde-17042012-115305/.

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O vermelho de alizarina S é um corante cuja cor é função do pH. É uma antraquinona sulfonada que possui dois grupos fenólicos ligados ao mesmo anel onde se encontra o grupo sulfônico. Usualmente é comercializado sob a forma de sal monossódico, também chamado alizarinossulfonato de sódio ou simplesmente vermelho de alizarina. Esse corante pode ser sintetizado pela oxidação e hidroxilação do antraceno, seguida de sua sulfonação. Ele é largamente empregado em histoquímica, análises quantitativas e em indústrias têxtil e de couros. Na indústria de couros, esse corante vem acompanhado de grandes ca
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Ferreira, Leila Soares. "Efeitos da fototerapia com laser em baixa intensidade e dos fatores de crescimento PDGF e BMP-2, isolados ou em associação, na diferenciação ósseo/odontogênica de células-tronco de polpa dentária humana." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/23/23134/tde-30112011-164711/.

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A fototerapia com laser em baixa intensidade (FTLBI) é capaz de aumentar o metabolismo celular, o que poderia influenciar na diferenciação ósseo/odontogênica das células-tronco da polpa dentária humada (hDPSCs). O PDGF e o BMP-2 são fatores de crescimento envolvidos na dentinogênese e na reparação tecidual. O PDGF tem papel importante durante o desenvolvimento embrionário, na proliferação e migração celular e na angiogênese, enquanto o BMP-2 está fortemente associado à diferenciação celular em tecidos mineralizados, como o osso e a dentina. Sendo assim, o objetivo do estudo foi analisar os efe
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Books on the topic "Alizarin Red S Staining"

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Kinnersley, A. D. Analysis of trace levels of aluminium in water by adsorptive stripping of the aluminium-alizarin red s complex at the hanging mercury drop electrode. UMIST, 1993.

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Book chapters on the topic "Alizarin Red S Staining"

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Holmgren, Allan, Willis Forsling, and Liuming Wu. "An FT-IR Study of Alizarin Red S Adsorbed at the Fluorapatite-Water Interface." In Progress in Fourier Transform Spectroscopy. Springer Vienna, 1997. http://dx.doi.org/10.1007/978-3-7091-6840-0_31.

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Studdert, Joshua B., Heidi Bildsoe, V. Pragathi Masamsetti, and Patrick P. L. Tam. "Visualization of the Cartilage and Bone Elements in the Craniofacial Structures by Alcian Blue and Alizarin Red Staining." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1847-9_4.

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Mukherjee, Tirthankar, Pradyumna Das, Sadhan Kumar Ghosh, and Mehabub Rahaman. "Removal of Alizarin Red S from Wastewater: Optimizing the Process Parameters for Electrocoagulation Using Taguchi Method." In Waste Water Recycling and Management. Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-2619-6_19.

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Man G.C.W., Yeung Y.H., Wang W.J., et al. "The Effect of Melatonin on Proliferation and Differentiation of Osteoblasts in Adolescent Idiopathic Scoliosis Vs Normal Control." In Studies in Health Technology and Informatics. IOS Press, 2008. https://doi.org/10.3233/978-1-58603-888-5-373.

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Low BMD and dysfunction of melatonin signaling pathway has been suggested as the possible key link to the spinal deformity in AIS. Prior studies shown that melatonin can influence and regulate skeletal growth and bone formation in human and animal models. However, there is a relative lack of direct evidence on the role of melatonin on AIS at the cellular and molecular level. To investigate the effect of melatonin on AIS osteoblasts at the cellular level. In vitro assays were performed with osteoblasts isolated from 7 girls with severe AIS and 7 controls. These were treated with different conce
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Vaddadi, Krishna, Nookaraju Muralasetti, and Naginami Naidu. "Efficacy of Cobalt-Incorporated Mesoporous Silica towards Photodegradation of Azodyes and Its Kinetic Study for Advanced Application." In Toxicity of Nanoparticles - Recent Advances and New Perspectives. IntechOpen, 2024. http://dx.doi.org/10.5772/intechopen.111520.

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Along with MCM-41, cobalt-incorporated mesoporous silica (Co-MCM-41) has been created. Powder X-ray diffraction, scanning electron microscopy, and nitrogen adsorption-desorption studies were used to describe the materials. It has been discovered that the Co-MCM-41 has less surface area (SBET, m2 g−1), pore volume (cc·g−1), and pore size (Å) than the MCM-41. The SEM-EDAX analysis has also unmistakably demonstrated the existence of the appropriate elements in the materials. The photoactivity was significantly impacted by the extremely distributed Co3+ species present on the MCM-41 structure. A t
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Conference papers on the topic "Alizarin Red S Staining"

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Kumaoka, M., T. Shiraishi, and S. Morishita. "Effects of Mechanical Vibration on Osteoblast-Like Cell Line." In ASME 2005 International Mechanical Engineering Congress and Exposition. ASMEDC, 2005. http://dx.doi.org/10.1115/imece2005-81980.

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Bone formation is subject in vivo to mechanical stimulation. Though many researches for bone cells of osteoblastic lineage sensing and responding to mechanical stimulation have been reported mainly in the biochemical field, the effects of mechanical stimulation are not so understood. In this study, in order to clarify the effects of mechanical vibration on osteoblast-like cells on the basis of the dynamic properties of the cells in the sense of vibration engineering, sinusoidal excitation was applied to the cells, and their cell proliferation and bone matrix generation were investigated as the
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Maynard, Jacqueline A., Ahmad S. Arabiyat, Anna Elefante, Lucas Shearer, Eoin King, and Andrea Kwaczala. "Using Acoustic Waves to Modulate Stem Cell Growth and Differentiation." In ASME 2017 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/imece2017-71341.

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During spaceflight, the loss of mechanical loads due to microgravity leads to rapid bone loss, where bone deteriorates at a rate of 1–2% per month, where some astronauts can lose as much as 20% of their skeletal mass in a single expedition [NASA, 2001]. In order to prevent muscle and bone loss, long-term space flight exercise regimes are strictly implemented [Shackleford, 2004]. Current research has demonstrated that mechanical vibrations can help to maintain or improve bone mass [Chan, 2013] and reduce adiposity [Chen, 2015, Sen, 2011] when signals are applied at the appropriate frequency and
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Shikata, Tetsuo, Toshihiko Shiraishi, Kumiko Tanaka, Shin Morishita, and Ryohei Takeuchi. "Effects of Acceleration Amplitude and Frequency of Mechanical Vibration on Osteoblast-Like Cells." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-41797.

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Bone formation is subject in vivo to mechanical stimulation. Although many researches for bone cells of osteoblastic lineage sensing and responding to mechanical stimulation have been reported mainly in the biochemical field, effects of mechanical stimulation on bone cells are not well understood. In this study, in order to clarify effects of acceleration amplitude and frequency of mechanical stimulation on MC3T3-E1, which is an osteoblast-like cell line derived from mouse calvaria, in the sense of mechanical vibrations, their cell proliferation, cell morphology, bone matrix generation and gen
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Shikata, Tetsuo, Toshihiko Shiraishi, Kumiko Tanaka, Shin Morishita, and Ryohei Takeuchi. "Effects of Amplitude and Frequency of Vibration Stimulation on Cultured Osteoblasts." In ASME 2007 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/detc2007-34949.

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Mechanical stimulation to bones affects osteogenesis such as decrease of bone mass of astronauts under zero gravity, walking rehabilitation to bone fracture and fracture repair with ultrasound devices. Bone cells have been reported to sense and response to mechanical stimulation at cellular level morphologically and metabolically. In the view of mechanical vibrations, bone cells are deformed according to mechanical stimulation and their mechanical characteristics. Recently, it was reported that viscoelasticity of cells was measured using tensile and creep tests and that there was likely natura
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Shikata, Tetsuo, Toshihiko Shiraishi, Shin Morishita, and Ryohei Takeuchi. "Effects of Acceleration Amplitude and Frequency of Mechanical Vibration on Cultured Osteoblasts." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-67221.

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This paper describes the effects of the frequency and acceleration amplitude of mechanical vibration on osteoblasts, the bone cells that generate the bone matrix. Their cell proliferation and bone matrix generation were investigated when sinusoidal inertia force was applied to the cells. Bone formation is subject in vivo to mechanical stimulation. Although many researches for bone cells of osteoblastic lineage sensing and responding to mechanical stimulation have been reported mainly in the biochemical field, effects of mechanical stimulation on bone cells are not well understood. After the ce
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Delgado, Ana, Pengfei Dong, Mahyar Sameti, et al. "Mechanical Characterization of Calcificaiton in Diseased Coronary Artery with Atomic Force Microscope." In 2022 Design of Medical Devices Conference. American Society of Mechanical Engineers, 2022. http://dx.doi.org/10.1115/dmd2022-1055.

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Abstract In this work, the mechanical propertied of calcification in diseased coronary artery was evaluated with atomic force microscope (AFM). The heavily calcified coronary artery was harvest from a cadaver’s heart. The artery slices with thickness of 10 um were prepared with cryosectioning. Staining with Alizarin Red has been performed to highlight the calcification region. Results have shown that the calcified areas have a significant larger stiffness compared with the surrounding plaque and the media layer of a healthy artery. The calcification showed a heterogeneous property with larger
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Rico-Mendez, Mario Angel, Romeo Selvas-Aguilar, Gerardo Alejandro Montano-González, et al. "Tapered optical fiber sensor of acid and alkaline solution with MWCNT and alizarin red S." In Novel Optical Systems, Methods, and Applications XXVI, edited by Cornelius F. Hahlweg and Joseph R. Mulley. SPIE, 2023. http://dx.doi.org/10.1117/12.2683629.

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Hussam Abdulwahab, Nuha. "Modifications to Erdogan Method to Visualization of Skeletal System Using Dual Pigmentation with Red Alizarin and Alcian Blue." In XII. International Scientific Congress of Pure, Applied and Technological Sciences. Rimar Academy, 2024. https://doi.org/10.47832/minarcongress12-11.

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Skeletal system dyeing is critical mode in anatomy to diagnose skeletal impairments. The point of this paper was to modernization dyeing and clearing mode of mice employing Alcian Blue- Alizarin Red S. The fetuses were, fixed, dyed, cleared, and preserved in glycerine with thymol crystals to prevent mold growth. This modulation stained the bone and the cartilage without harming the skeleton
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Pradeeba, S. J., and K. Sampath. "Photodegradation efficiency of methyl orange and Alizarin Red S in waste water using poly(azomethine)/TiO2 nanocomposite." In PROCEEDINGS OF THE INTERNATIONAL CONFERENCE ON ADVANCED MATERIALS: ICAM 2019. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5130238.

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Aissat, Miloud, Sara Hamouda, Naceur Benhadria, Rachid Chellali, and Noureddine Bettahar. "Alizarin red S dye removal from contaminated water on calcined [Mg/Al, Zn/Al and MgZn/Al]-LDH." In TECHNOLOGIES AND MATERIALS FOR RENEWABLE ENERGY, ENVIRONMENT AND SUSTAINABILITY: TMREES18. Author(s), 2018. http://dx.doi.org/10.1063/1.5039164.

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Reports on the topic "Alizarin Red S Staining"

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มาลัยวิจิตรนนท์, สุจินดา, та วัชราภรณ์ ติยะสัตย์กุลโกวิท. การศึกษาผลและกลไกการรักษาภาวะกระดูกพรุนของพืชสมุนไพรไทยกวาวเครือขาวในหนูแรท : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2013. https://doi.org/10.58837/chula.res.2013.45.

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การวิจัยนี้เพื่อศึกษากลไกการออกฤทธิ์ของสารไฟโตเอสโตรเจนที่สกัดได้จากกวาวเครือขาวต่อกระบวนการสร้างและสลายกระดูก และการออกฤทธิ์ผ่านตัวรับของฮอร์โมนเอสโตรเจนในเซลล์สร้างกระดูก osteoblast ของหนูแรท ในหลอดทดลอง โดยทำการทดลองในเซลล์ 2 ชนิด คือ osteosarcoma cell line UMR-106 และ primary rat osteoblast cells และให้สาร genistein (GEN) ที่ความเข้มข้น 0.1, 10 และ 1000 nmol/L, puerarin (PU) ที่ความเข้มข้น 1, 10 และ 100 ug/mL นาน 48 ชั่วโมง เทียบผลการทดลองที่ได้กับ 17β-Estradiol ที่ความเข้มข้น 10 nmol/L (กลุ่มควบคุมเชิงบวก) และ 0.3% DMSO (กลุ่มควบคุมเชิงลง) พบว่าสารสกัดกวาวเครือขาวและสารไฟโตเอสโตรเจนที่พบใ
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