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Journal articles on the topic 'Alizarin Red S Staining'

Author: Grafiati

Published: 3 June 2025

Last updated: 1 August 2025

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1

Caraguel, Jean-Marie, Thomas Barreau, Sarah Brown-Vuillemin, and Samuel P. Iglésias. "In vivo staining with alizarin for ageing studies on chondrichthyan fishes." Aquatic Living Resources 33 (2020): 1. http://dx.doi.org/10.1051/alr/2020002.

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Age determination for stock assessments and conservation of cartilaginous fishes is mainly obtained by counting the annual growth bands in vertebrae. Recent studies show numerous inconsistencies and the need for systematic validation. We assessed the effectiveness of the fluorochrome alizarin red S, a common skeleton vital marker used as a time stamp for teleost fishes, on chondrichthyan. Twenty-five captive small-spotted catsharks (Scyliorhinus canicula) were marked by alizarin red S intraperitoneal injections. The fluorochrome produced a wide fluorescent mark on sectioned vertebral centra of

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Luthfi, Muhammad Ja’far. "Modified Alizarin Red S-Alcian Blue Staining for Reptilian Skeleton." Biology, Medicine, & Natural Product Chemistry 5, no. 1 (2016): 19. http://dx.doi.org/10.14421/biomedich.2016.51.19-22.

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<p>Skeletal staining is an important method in anatomical study. The aim of the research was to develop staining and clearing method of Reptilian skeleton using Alizarin Red S-Alcian Blue. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. This modified double-staining has successfully stain bone and cartilage of Reptilian.</p>

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White, Kristopher, Rabab Chalaby, Gina Lowe, Jacob Berlin, Carlotta Glackin, and Ronke Olabisi. "Calcein Binding to Assess Mineralization in Hydrogel Microspheres." Polymers 13, no. 14 (2021): 2274. http://dx.doi.org/10.3390/polym13142274.

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We describe a method to assess mineralization by osteoblasts within microspheres using calcein. Fluorescence imaging of calcein bound to the calcium in hydroxyapatite permits assessment of the mineralized portion of the extracellular matrix. Colorimetric imaging of Alizarin Red S complexed with calcium also gives measures of mineralization, and in tissue cultures calcein and Alizarin Red S have been shown to bind to the same regions of mineral deposits. We show that when the mineralization takes place within hydrogel microspheres, Alizarin Red S does not stain mineral deposits as consistently

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Bernar, Aline, Jennifer Viktoria Gebetsberger, Monika Bauer, Werner Streif, and Michael Schirmer. "Optimization of the Alizarin Red S Assay by Enhancing Mineralization of Osteoblasts." International Journal of Molecular Sciences 24, no. 1 (2022): 723. http://dx.doi.org/10.3390/ijms24010723.

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The alizarin red S assay is considered the gold standard for quantification of osteoblast mineralization and is thus widely used among scientists. However, there are several restrictions to this method, e.g., moderate sensitivity makes it difficult to uncover slight but significant effects of potentially clinically relevant substances. Therefore, an adaptation of the staining method is appropriate and might be obtained by increasing the mineralization ability of osteoblasts. In this study, cell culture experiments with human (SaOs-2) and murine (MC3T3-E1) osteoblasts were performed under the a

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Afshar, M., N. Taibi, and M. J. Golalipoor. "Tu-P7:286 Modified alizarin red S staining to detect vascular calcification." Atherosclerosis Supplements 7, no. 3 (2006): 247–48. http://dx.doi.org/10.1016/s1567-5688(06)80989-x.

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6

Mori, Fumiaki, and Goyo Koya. "CEREBELLAR CALCIFICATION DETECTED BY ALIZARIN RED S STAINING IN THIOPHENE-EXPOSED RATS." Journal of Toxicologic Pathology 8, no. 4 (1995): 391–99. http://dx.doi.org/10.1293/tox.8.391.

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7

Rakhmiyati, Rakhmiyati, and Muhammad Ja’far Luthfi. "Alizarin Red S-Alcian Blue Staining for Regenerated tail of Common House Gecko (Hemidactylus frenatus)." Biology, Medicine, & Natural Product Chemistry 7, no. 2 (2018): 57–59. http://dx.doi.org/10.14421/biomedich.2018.72.57-59.

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Common House Gecko (Hemidactylus frenatus) is one of reptiles that have ability to autotomy their tails. Tail autotomy is a mechanism to protect it self from predators. After the tail broke, there will be wound healing on the tail which is then followed by a tail regeneration event. Original tail and regenerate tail is very different morphologically and anatomically. The original tail is composed of bones while the tail of the regenerate is composed of cartilage. Histochemical staining using Alizarin Red-S Alcian Blue was done to differentiate bone and cartilage. This method will stained bones

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Kim, Da-Sol, Soo-Kyung Bae, Yeon-Ju Kwak, Geum-Joung Youn, and Hye-Ock Jang. "Osteogenic Potential of Osteolforte: Gene and Protein-Level Evaluation in Human Bone Marrow Stromal Cells." Current Issues in Molecular Biology 47, no. 8 (2025): 588. https://doi.org/10.3390/cimb47080588.

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Osteolforte, a compound with potential bone-regenerative properties, was investigated for its effects on human bone marrow stromal cells (hBMSCs). This study aimed to evaluate its impact on cell viability, osteogenic differentiation, and both gene and protein expression using a combination of assays, including CCK-8, Alizarin Red S staining, Quantitative Real-Time PCR (qRT-PCR), and Western blot analysis. The results demonstrated that Osteolforte significantly enhanced osteogenic differentiation in hBMSCs. Alizarin Red S staining revealed increased mineralization, indicating elevated calcium d

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BUI, E. N., and A. R. MERMUT. "QUANTIFICATION OF SOIL CALCIUM CARBONATES BY STAINING AND IMAGE ANALYSIS." Canadian Journal of Soil Science 69, no. 3 (1989): 677–82. http://dx.doi.org/10.4141/cjss89-066.

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Quantification of calcium carbonates in a light-colored matrix or disseminated calcium carbonates cannot be obtained directly from impregnated blocks by image analysis. A method that combined staining by alizarin red-S with digital image analysis made it possible to identify and quantify soil calcium carbonates including the disseminated form in thin sections. Best results were obtained under reflected light using a tungsten lamp source. Photographic filters did not improve the image quality of stained calcium carbonates. Key words: Image analysis, soil micromorphometry

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Luthfi, Muhammad Ja’far, and Nyoman Puniawati Soesilo. "A Simple Method for Clearing and Staining Specimens for The Demonstration of Animal Skeleton." Biology, Medicine, & Natural Product Chemistry 8, no. 1 (2019): 17–21. http://dx.doi.org/10.14421/biomedich.2019.81.17-21.

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Teaching skeletal system would be more attractive using real/preserved specimen compared to those using only book. The aim of the research was to develop clearing and staining method of animal skeleton specimen using Alizarin Red S-Alcian Blue as teaching aid tool. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. The spesimen will increase effectiveness in elementary school science teaching and learning.

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Dar, Firdous Ahmad, Masuood Ahmad John, Andleeb Rafiq, N. Shazia, and Abdur Rezzaque Choudhary. "Taxidermy and Alizarin Red S-Alcian Blue Staining of Levantine Viper snake (Macrovipera lebetina)." SKUAST Journal of Research 24, no. 2 (2022): 269–70. http://dx.doi.org/10.5958/2349-297x.2022.00031.9.

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Suzuki, Kazuhiro, and Koichi Mimura. "Alizarin Red S staining method for the effective detection of conodont elements in limestone." Journal of the Geological Society of Japan 118, no. 4 (2012): 236–39. http://dx.doi.org/10.5575/geosoc.2011.0030.

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13

Pan, Mengxue, Wei Hong, Ye Yao, et al. "Activated B Lymphocyte Inhibited the Osteoblastogenesis of Bone Mesenchymal Stem Cells by Notch Signaling." Stem Cells International 2019 (June 2, 2019): 1–14. http://dx.doi.org/10.1155/2019/8150123.

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Estrogen is very important to the differentiation of B lymphocytes; B lymphopoiesis induced by OVX was supposedly involved in osteoporosis. But the effects of B lymphocytes on the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) are not clear. In this study, we detected bone quality and bone loss in a trabecular bone by electronic universal material testing machine and microcomputed tomography (micro-CT) in OVX and splenectomized-ovariectomy (SPX-OVX) rats. Additionally, changes in lymphocytes (B lymphocyte, CD4+ and CD8+ T lymphocytes, and macrophages) in the bone marrow were

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Alnahash, Abdullah, Young-Min Song, Sae-Kyung Min, et al. "Effects of Connective Tissue Growth Factor on the Cell Viability, Proliferation, Osteogenic Capacity and mRNA Expression of Stem Cell Spheroids." Applied Sciences 11, no. 14 (2021): 6572. http://dx.doi.org/10.3390/app11146572.

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Background: Connective tissue growth factor (CTGF) is a cellular communication network factor family protein involved in many cellular functions. The purpose of this study was to determine the effects of CTGF on the proliferation, osteogenic capacity, and mRNA expression of spheroids composed of gingiva-derived mesenchymal stem cells (GMSCs). Methods: CTGF was applied at final concentrations of 0, 25, 50, 100, and 200 ng/mL. Qualitative cell viability was determined using Live/Dead kit assay. Metabolic viability was determined with a colorimetric assay kit. Osteogenic activity was analyzed wit

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15

Chen, Tianlei, Huijuan Mao, Cheng Chen та ін. "The Role and Mechanism ofα-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells". BioMed Research International 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/194362.

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Objective. To investigate the role and possible mechanism ofα-Klotho in the calcification and the osteogenic transition of cultured VSMCs.Methods. VSMCs were culturedin vitroand divided into 5 groups, each using a different medium: (1) control; (2)β-GP; (3)β-GP + Klotho; (4)β-GP + LiCl; (5)β-GP + Klotho + LiCl. Calcium deposits were visualized using Alizarin Red S staining. The calcium concentrations were determined by the o-cresolphthalein complexone method. BMP2, Runx2 andβ-catenin levels were estimated by western blotting, and the level ofα-SMA was determined by using immunofluorescence at

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Rho, Keonho, Chan Park, Khurshed Alam, et al. "Biological Effects of Plasma-Based Graphene Oxide Deposition on Titanium." Journal of Nanomaterials 2019 (November 3, 2019): 1–7. http://dx.doi.org/10.1155/2019/9124989.

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This study was performed to investigate the effects of argon plasma treatment under atmospheric pressure at room temperature on the cytotoxicity and antimicrobial effects of the graphene oxide layer on titanium. Plasma treatment of the graphene oxide coating on a nonthermal atmospheric-pressure plasma device was performed. Raman spectrum analysis confirmed that graphene oxide was successfully coated on the surface and AFM analysis confirmed that this coating affected the surface roughness. X-ray photoelectron spectroscopy (XPS), Alizarin Red S staining for cell differentiation, and Raman and a

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Mrak, Polona, Nada Žnidaršič, and Jasna Štrus. "Alizarin red S staining of the crustacean cuticle: implementation in the study of Porcellio scaber larvae." Acta Biologica Slovenica 56, no. 2 (2013): 51–61. http://dx.doi.org/10.14720/abs.56.2.16125.

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Exoskeletal cuticle of crustaceans is a chitinous matrix, produced apically by epidermis and stiffened by sclerotization and calcification. Embryos of terrestrial isopod crustacean Porcellio scaber develop within the female brood pouch, marsupium, and after hatching larvae mancae continue their development in the marsupium for another week. This study was performed to reveal at the histochemical level whether the exoskeletal cuticle of marsupial mancae is already calcified. Fifteen different procedures of histochemical staining with alizarin red S (ARS), established for calcified tissue locali

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18

Zainal Ariffin, Shahrul Hisham, Rohaya Megat Abdul Wahab, Muhammad Abdul Razak, et al. "Evaluation of in vitro osteoblast and osteoclast differentiation from stem cell: a systematic review of morphological assays and staining techniques." PeerJ 12 (July 25, 2024): e17790. http://dx.doi.org/10.7717/peerj.17790.

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Background Understanding human stem cell differentiation into osteoblasts and osteoclasts is crucial for bone regeneration and disease modeling. Numerous morphological techniques have been employed to assess this differentiation, but a comprehensive review of their application and effectiveness is lacking. Methods Guided by the PRISMA framework, we conducted a rigorous search through the PubMed, Web of Science and Scopus databases, analyzing 254 articles. Each article was scrutinized against pre-defined inclusion criteria, yielding a refined selection of 14 studies worthy of in-depth analysis.

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Yu, Zi-Li, Bin-Fang Jiao, and Zu-Bing Li. "Lysophosphatidic Acid Analogue rather than Lysophosphatidic Acid Promoted the Bone Formation In Vivo." BioMed Research International 2018 (May 29, 2018): 1–10. http://dx.doi.org/10.1155/2018/7537630.

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Lysophosphatidic acid (LPA), a bioactive lipid molecule, has recently emerged as physiological and pathophysiological regulator in skeletal biology. Here we evaluate the effects of LPA on bone formation in vivo in murine femoral critical defect model. Primary femoral osteoblasts were isolated and treated with osteogenic induction conditional media supplemented with 20 μM LPA or LPA analogue. Mineralized nodules were visualized by Alizarin Red S staining. Forty-five C57BL/6 mice underwent unilateral osteotomy. The femoral osteotomy gap was filled with porous scaffolds of degradable chitosan/bet

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Yamada, Takashi. "Selective staining methods for cartilage of rat fetal specimens previously treated with alizarin red S." Teratology 43, no. 6 (1991): 615–19. http://dx.doi.org/10.1002/tera.1420430617.

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21

Young, Angela D., Donal E. Phipps, and A. Barry Astroff. "Large-scale double-staining of rat fetal skeletons using Alizarin Red S and Alcian Blue." Teratology 61, no. 4 (2000): 273–76. http://dx.doi.org/10.1002/(sici)1096-9926(200004)61:4<273::aid-tera5>3.0.co;2-2.

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22

Nofita, Putri, Muhammad Ja’far Luthfi, and Reza Sukma Dewi. "Anatomical Study of Red-eared TurteTail (Trachemys scripta elegans)." Proceeding International Conference on Science and Engineering 2 (March 1, 2019): 137–40. http://dx.doi.org/10.14421/icse.v2.72.

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Turtles are reptiles armored backs hard, slow motion, appearing for the first time about 200 million years ago and relative ly unchanged for 150 million years. This study aims to determine the anatomical structure of the axial skeleton and determine the histologic structure of red-eared turtles (Trachemys scripta elegans) females. The method used, among others, Alizarin Red S staining Alcian Blue, methods of paraffin with Hematoxylin-eosin staining (HE) and Mallory acid fuchsin and used 3 adult turtles 3 years old. The results showed that vertebral caudalis red-eared turtles (Trachemys scripta

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23

Ghezzi, Benedetta, Ludovica Parisi, Elena Calciolari, et al. "Testosterone Enanthate: An In Vitro Study of the Effects Triggered in MG-63 Cells." Biomolecules 12, no. 8 (2022): 1159. http://dx.doi.org/10.3390/biom12081159.

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The aim of this study was to investigate the effects of the androgenic hormone testosterone enanthate (TE) on human MG-63 cells. MG-63 were cultured for 24 h in the presence of TE at increasing concentrations to assess its lethal dose. Therefore, the suitable concentration for a prolonged use of TE in vitro was assessed by viability assay over 9 days. Finally, MG-63 were exposed to TE for 14 days and assayed for differentiation by qPCR and Alizarin Red S staining. TE in the amount of 100 µM resulted as the maximum dose tolerated by MG-63 cells after 24 h. However, a prolonged exposure in cultu

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Kheiry, Elahe Vadaye, Kazem Parivar, Javad Baharara, and Alireza Iranbakhsh. "The Osteogenic Differentiation of Mice Bone Marrow Mesenchymal Stem Cells with Fisetin Phytoestrogen." Journal of Molecular Biology Research 7, no. 1 (2017): 162. http://dx.doi.org/10.5539/jmbr.v7n1p162.

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Objective(s): The aim of this study was to evaluate the effects of fisetin to promote osteogenic differentiation in mice bone marrow mesenchymal stem cells (BMSCs).Materials and Methods: In this study cytotoxicity and viability of fisetin was measured by MTT assay. The differentiation effects of fisetin on BMSCs into osteoblast was assessed with alkaline phosphatase (ALP) activity measurement. Alizarin red staining and Real time PCR for osteoblast specific marker, Osteocalcin (OCN) ,Osteopontin (OPN), Runt-related transcription factor 2 (RUNX2), ERK and MAPK were investigatedResults: The resul

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Kim, Mihyang, Jeong Hyeon Kang, Geun Hye Oh, and Mi Hwa Park. "Ishige sinicola extract stimulates osteoblast proliferation and differentiation via the bone morphogenetic protein 2/runt-related gene 2 signalling pathway." Zeitschrift für Naturforschung C 74, no. 7-8 (2019): 167–74. http://dx.doi.org/10.1515/znc-2018-0044.

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Abstract Osteoporosis is one of the most common bone diseases, occurring due to an imbalance between bone formation and bone resorption. The aim of this study was to investigate the effects of Ishige sinicola, a brown alga, on osteoblast differentiation through the activation of the bone morphogenetic protein 2 (BMP-2)/runt-related transcription factor 2 (Runx2) signalling pathway in MC3T3-E1 cells. A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining, and expression analysis of osteoblastic genes were carried out to assess MC3T3-E1 cell proliferation an

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Palmieri, Valentina, Marta Barba, Lorena Di Pietro, et al. "Graphene Oxide Induced Osteogenesis Quantification by In-Situ 2D-Fluorescence Spectroscopy." International Journal of Molecular Sciences 19, no. 11 (2018): 3336. http://dx.doi.org/10.3390/ijms19113336.

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Graphene and graphene oxide can promote the adhesion, growth and differentiation of mesenchymal stem cells. Further, graphene surface coatings accelerate the differentiation of human mesenchymal stem cells acting as osteogenic inducers. Quantification of the osteogenic induction is conventionally performed with Alizarin Red S (ARS), an anthraquinone derivative used to identify calcium deposits in tissue sections and cell cultures. The ARS staining is quite versatile because the dye forms an Alizarin Red S–calcium complex that can be extracted from the stained monolayer of cells and readily ass

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Shipulina, S. A., I. A. Goncharova, D. S. Panfilov, B. N. Kozlov, I. V. Sukhodolo, and M. S. Nazarenko. "Specific features of aortic wall calcification in patients with thoracic aortic aneurysm." YAKUT MEDICAL JOURNAL 84, no. 4 (2023): 13–16. https://doi.org/10.25789/ymj.2023.84.03.

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Histological assessment of calcification patterns in different sites of ascending aorta in patients with thoracic aortic aneurysm has been performed using alizarin red S staining. Biopsy specimens from 67 patients (47 males, 20 females, mean age 56,9±11,1 years) with thoracic aortic aneurysm from three different sites of ascending aorta were taken intraoperatively. Calcium content in aorta was calculated as the percentage of positively stained area using FiJi. Statistical data processing was performed using JASP 0.17.2.1. Calcific precipitates were diffusely distributed mainly in the tunica me

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Li, Ruixue, Jian Sun, Fei Yang та ін. "Effect of GARP on osteogenic differentiation of bone marrow mesenchymal stem cells via the regulation of TGFβ1 in vitro". PeerJ 7 (23 травня 2019): e6993. http://dx.doi.org/10.7717/peerj.6993.

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Mesenchymal stem cells (MSCs), which have multipotential differentiation and self-renewal potential, are possible cells for tissue engineering. Transforming growth factor β1 (TGFβ1) can be produced by MSCs in an inactive form, and the activation of TGFβ1 functions as an important regulator of osteogenic differentiation in MSCs. Recently, studies showed that Glycoprotein A repetitions predominant (GARP) participated in the activation of latent TGFβ1, but the interaction between GARP and TGFβ1 is still undefined. In our study, we successfully isolated the MSCs from bone marrow of rats, and showe

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Choi, Minji, Jiyoung Kwon, Ji-Hyun Jang, Duck-Su Kim, and Hyun-Jung Kim. "Enhancing the Biological Properties of Organic–Inorganic Hybrid Calcium Silicate Cements: An In Vitro Study." Journal of Functional Biomaterials 15, no. 11 (2024): 337. http://dx.doi.org/10.3390/jfb15110337.

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(1) Background: This study aimed to enhance the biological properties of hydraulic calcium silicate cements (HCSCs) by incorporating organic and inorganic components, specifically elastin-like polypeptides (ELPs) and bioactive glass (BAG). We focused on the effects of these composites on the viability, migration, and osteogenic differentiation of human periodontal ligament fibroblasts (hPDLFs). (2) Methods: Proroot MTA was supplemented with 1–5 wt% 63S BAG and 10 wt% ELP. The experimental groups contained various combinations of HSCS with ELP and BAG. Cell viability was assessed using an MTT a

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Springer, Victor G., and G. David Johnson. "Use and Advantages of Ethanol Solution of Alizarin Red S Dye for Staining Bone in Fishes." Copeia 2000, no. 1 (2000): 300–301. http://dx.doi.org/10.1643/0045-8511(2000)2000[0300:uaaoes]2.0.co;2.

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Na, WooJin, and Young-Hee Kang. "Aesculetin Promotes Osteoblastogenic Bone Formation Through Enhancing Osteoblast Differentiation and Mineralization." Current Developments in Nutrition 5, Supplement_2 (2021): 350. http://dx.doi.org/10.1093/cdn/nzab037_060.

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Abstract Objectives Osteoporosis is a common chronic disease elicited by imbalance between osteoblastic bone formation and osteoclastic bone resorption. Marked increase in bone resorption leads to the aberrant fall in bone mineral density. With increasing age, there is also a significant reduction in bone formation. Aesculetin, a derivative of coumarin, possesses anti-inflammatory and antioxidant effects. The purpose of this study was to identify that aesculetin accelerated bone formation through increased osteoblastic differentiation and mineralization. Methods MC3T3-E1 cells were cultured wi

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Valanezhad, Alireza, Tetsurou Odatsu, Shigeaki Abe, and Ikuya Watanabe. "Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells." International Journal of Molecular Sciences 22, no. 22 (2021): 12259. http://dx.doi.org/10.3390/ijms222212259.

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Recently, ferroptosis has gained scientists’ attention as an iron-related regulated necrosis. However, not many reports have investigated the effect of ferroptosis on bone. Therefore, with the present study, we assessed the effect of ferroptosis inhibition using ferrostatin-1 on the MC3T3-E1 pre-osteoblast cell. Cell images, cell viability, alkaline phosphatase activity test, alizarin red staining, and RUNX2 gene expression using real-time PCR were applied to investigate the effects of ferrostatin and erastin on MC3T3-E1 osteoblast cells. Erastin was used as a well-known ferroptosis inducer re

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Chen, Haiying, Lei Zhang, and Lijun Tao. "miR-143 Inhibits Osteogenic Differentiation of Umbilical Cord Mesenchymal Stem Cell by Targeting Zinc Fingers and Homeoboxes 3." Journal of Biomaterials and Tissue Engineering 9, no. 11 (2019): 1550–56. http://dx.doi.org/10.1166/jbt.2019.2183.

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The transcription regulating factor-zinc fingers and homeoboxes 3 (ZHX3) involve in osteoblast differentiation. miR-143 regulates osteoblast differentiation. Bioinformatics analysis revealed a relationship between miR-143 and the 3′ -UTR of ZHX3. This study assessed miR-143's role in UC-MSC differentiation. The UC-MSCs were induced to differentiate into osteoblasts. The expressions of miR-143, ZHX3, RUNX2, Osteopontin (OPN) and Osteocalcin (OC) genes were detected by qRTPCR on days 0, 20 and 30. The UC-MSCs cells were divided into agomir-NC group and miR-143 agomir group. After 30 days of diff

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Bagge, Jasmin, Haider Mahmood, Jennifer Janes, et al. "Chondrogenic and Osteogenic In Vitro Differentiation Performance of Unsorted and Sorted CD34+, CD146+, and CD271+ Stem Cells Derived from Microfragmented Adipose Tissue of Patients with Knee Osteoarthritis." Journal of Clinical Medicine 14, no. 4 (2025): 1184. https://doi.org/10.3390/jcm14041184.

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Background/Objectives: Treatment of knee osteoarthritis (OA) with autologous stem cells from microfragmented adipose tissue (MFAT) has shown promising but varying results. Multiple stem cell types, including CD34+, CD146+, and CD271+ stem cells, have been identified within MFAT. Patient-specific heterogeneity in stem cell populations and the content of highly potent cells may be determining factors for a successful treatment outcome. The current study aimed to identify the most promising stem cell type in MFAT to treat OA, focusing on their chondrogenic and osteogenic differentiation performan

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Li, Shihuan, Qingjie Li, Qiaofeng Zhou та ін. "Attenuating Atherosclerosis through Inhibition of the NF-κB/NLRP3/IL-1β Pathway-Mediated Pyroptosis in Vascular Smooth Muscle Cells (VSMCs)". Cardiovascular Therapeutics 2024 (25 березня 2024): 1–18. http://dx.doi.org/10.1155/2024/1506083.

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Objective. We investigated the effects of resveratrol (Res) and MCC950 on the pyroptosis of vascular smooth muscle cells (VSMCs) and the potential pathway. Methods and Results. Compared with the control (Con) group, the atherosclerosis (AS) group showed calcified nodules, which suggested that the calcification medium induced the calcification of VSMCs. VSMCs showed proliferative activity and significantly attenuated calcification under treatment with 10 μmol/L Res. The calcium salt was detected by alizarin red S staining. Res and MCC950 downregulated the calcification, inflammatory, pyroptosis

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Ardiana, Ardiana, Muhammad Ja’far Luthfi, Nyoman Puniawati Soesilo, Laila Ainun Nisa, and Risti Widyaningsih. "Macro and Microanatomical Structures of Lizard’s Regenerate Tail (Mabouya multifasciata Kuhl, 1820)." Proceeding International Conference on Science and Engineering 2 (March 1, 2019): 25–29. http://dx.doi.org/10.14421/icse.v2.49.

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Lizards are animals that can do autotomy. Research on the autotomy of lizard’s tail has been carried out, but few have examined about axial skeletons that focus on vertebrae caudales or arranged muscles. That is the background of this research. This research is expected to be a database for further research and as a comparison between animals that can perform other autotomies. This research intend to determine the macro and micro structures of the anatomical axial skeleton lizard’s regenerate tail and to know the micro structures of anatomy muscular segmentation lizard’s regenerate tail. The u

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Moriguchi, T., K. Yano, S. Nakagawa, and F. Kaji. "Elucidation of adsorption mechanism of bone-staining agent alizarin red S on hydroxyapatite by FT-IR microspectroscopy." Journal of Colloid and Interface Science 260, no. 1 (2003): 19–25. http://dx.doi.org/10.1016/s0021-9797(02)00157-1.

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Sun, Yuqi, and Yuanbo Wang. "Calcium-based nanowires for bone repair." Journal of Physics: Conference Series 2838, no. 1 (2024): 012009. http://dx.doi.org/10.1088/1742-6596/2838/1/012009.

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Abstract Bone defects due to the older population, severe trauma, congenital malformations, resection of bone tumors, and infections are serious problems faced in orthopedic clinical treatment, posing great hazards to human health and life, and external interventions are generally required to promote bone healing. Considering the osteogenic promotion of calcium ions, Ca-POM nanowires were synthesized in this study to facilitate the osteogenesis process through the release of calcium ions. The cytotoxicity test showed that the material possesses good biocompatibility. Furthermore, the Alizarin

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Wu, Mei, Hongfeng Ge, Shue Li, et al. "Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors." Journal of Immunology Research 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/316351.

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Mesenchymal stem cells are immunoregulation cells. IL-22 plays an important role in the pathogenesis of immune thrombocytopenia. However, the effects of mesenchymal stem cells on IL-22 production in patients with immune thrombocytopenia remain unclear. Flow cytometry analyzed immunophenotypes of mesenchymal stem cells; differentiation of mesenchymal stem cells was observed by oil red O and Alizarin red S staining; cell proliferation suppression was measured with MTS; IL-22 levels of cell-free supernatants were determined by ELISA. Mesenchymal stem cells inhibited the proliferation of activated

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Liu, Yijuan, Lina Fan, Xuemei Lin, et al. "Functionalized self-assembled peptide RAD/Dentonin hydrogel scaffold promotes dental pulp regeneration." Biomedical Materials 17, no. 1 (2021): 015009. http://dx.doi.org/10.1088/1748-605x/ac3928.

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Abstract RADA16-I is an ion-complementary self-assembled peptide with a regular folded secondary conformation and can be assembled into an ordered nanostructure. Dentonin is an extracellular matrix phosphate glycoprotein functional peptide motif-containing RGD and SGDG motifs. In this experiment, we propose to combine RAD and Dentonin to form a functionalized self-assembled peptide RAD/Dentonin hydrogel scaffold. Furthermore, we expect that the RAD with the addition of functional motif Dentonin can promote pulp regeneration. The study analyzed the physicochemical properties of RAD/Dentonin thr

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Lee, J. H., Y. M. Lee, G. H. Maeng, S. L. Lee, and G. J. Rho. "303 IN VITRO NEURONAL DIFFERENTIATION OF MESENCHYMAL STEM CELLS DERIVED FROM CANINE EAR SKIN." Reproduction, Fertility and Development 23, no. 1 (2011): 248. http://dx.doi.org/10.1071/rdv23n1ab303.

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The canine has been a useful animal model for the study of fundamental mechanisms and the testing of new therapies for several human pathologies using mesenchymal stem cells (MSC). For preclinical applications, the most commonly used source of canine MSC is bone marrow. Because the amount of autologous bone marrow that can be obtained is limited, skin tissue could supply a noninvasive alternative with large quantities available for the establishment of MSC. In this study, we isolated canine MSC (cMSC) from ear skin and evaluated the expression of transcriptional factors and in vitro differenti

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Rosa-Molinar, E. "Imaging Techniques Combined With Enzyme-Based Procedures That Facilitate the Simultaneous Imaging of Nerves, Neurons, Cartilage And Bones During Development In Gambusia." Microscopy and Microanalysis 5, S2 (1999): 1074–75. http://dx.doi.org/10.1017/s1431927600018699.

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A persistent problem in elucidating the anatomy of the peripheral nervous system has been an inability to stain both myelinated and unmyelinated nerve fibers. To overcome this problem, our laboratory developed two workmg protocols for reliably and differentially labeling and staining the peripheral nervous system and combined them with an enzyme clearing and staining procedure for the simultaneous visualization of bone and cartilage.One protocol uses anti-acetylated α-tubulin immunohistochemistry to follow the course, peripheral branching, and origin of the ventral spinal nerve innervating the

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YAMADA, Takashi, Akiyoshi KURIHARA, Tsutomu NISHIYAMA, and Hideo UCHIDA. "Application of the Bromophenol Blue (BPB) Staining Method to Rat Fetal Cartilage Previously Stained with Alizarin Red S." Experimental Animals 42, no. 3 (1993): 457–61. http://dx.doi.org/10.1538/expanim1978.42.3_457.

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Atkins, Jade B., Laurent Houle, Alanna S. Cantelon, and Hillary C. Maddin. "Normal development in Ambystoma mexicanum : A complementary staging table for the skull based on Alizarin red S staining." Developmental Dynamics 249, no. 5 (2020): 656–65. http://dx.doi.org/10.1002/dvdy.152.

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Wang, Junsheng, Xiaowu Hu, Qing Ji, Bai Zheng та Lixin Huang. "miR-150 Affects Wnt/β-Catenin Pathway Activity and Regulates MSC Osteogenic Differentiation". Journal of Biomaterials and Tissue Engineering 9, № 10 (2019): 1339–45. http://dx.doi.org/10.1166/jbt.2019.2147.

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The Wnt/β-catenin pathway promotes osteoblast differentiation. Bioinformatics analysis showed a targeting relationship between miR-150 and β-catenin 3′-UTR. This study investigated miR-150's role in β-catenin expression and osteoblast differentiation of Adipose-Derived Mesenchymal Stem Cells (ADMSCs). Differentiation of ADMSCs into osteoblasts was induced and the expression of miR-150, β-catenin, RUNX2 and OPN on the 0th, 9th and 18th day during differentiation was detected. The dual luciferase gene reporter assay validated the relationship between miR-150 and β-catenin. ADMSCs were divided in

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Rady, Dina, Nassreen Albar, Waad Khayat, et al. "Evaluation of dental pulp stem cells response to flowable nano-hybrid dental composites: A comparative analysis." PLOS ONE 19, no. 5 (2024): e0303154. http://dx.doi.org/10.1371/journal.pone.0303154.

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Background Flowable resin composites (FRC) are tooth-colored restorative materials that contain a lower filler particle content, and lower viscosity than their bulk counterparts, making them useful for specific clinical applications. Yet, their chemical makeup may impact the cellular population of the tooth pulp. This in-vitro study assessed the cytocompatibility and odontogenic differentiation capacity of dental pulp stem cells (DPSCs) in response to two recent FRC material extracts. Methods Extracts of the FRC Aura easyflow (AEF) and Polofil NHT Flow (PNF) were applied to DPSCs isolated from

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Su, Shu-Jem, Yao-Tsung Yeh, Shu-Hui Su, et al. "Biochanin A Promotes Osteogenic but Inhibits Adipogenic Differentiation: Evidence with Primary Adipose-Derived Stem Cells." Evidence-Based Complementary and Alternative Medicine 2013 (2013): 1–12. http://dx.doi.org/10.1155/2013/846039.

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Biochanin A has promising effects on bone formationin vivo, although the underlying mechanism remains unclear yet. This study therefore aimed to investigate whether biochanin A regulates osteogenic and adipogenic differentiation using primary adipose-derived stem cells. The effects of biochanin A (at a physiologically relevant concentration of 0.1–1 μM) were assessedin vitrousing various approaches, including Oil red O staining, Nile red staining, alizarin red S staining, alkaline phosphatase (ALP) activity, flow cytometry, RT-PCR, and western blotting. The results showed that biochanin A sign

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Lee, Hyunjin, Youngmin Song, Yoon-Hee Park, Md Salah Uddin, and Jun-Beom Park. "Evaluation of the Effects of Cuminum cyminum on Cellular Viability, Osteogenic Differentiation and Mineralization of Human Bone Marrow-Derived Stem Cells." Medicina 57, no. 1 (2021): 38. http://dx.doi.org/10.3390/medicina57010038.

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Background and Objectives: Cuminum cyminum L. has long been used in the treatment of various diseases in multiple geographical regions. This study was performed to determine the effects of C. cyminum methanolic extract (CCT) on the cellular viability, alkaline phosphatase activity and mineralization of human mesenchymal stem cells. Materials and Methods: Bone marrow-derived stem cells were cultured in the presence of CCT at concentrations of 0, 0.001, 0.01, 0.1 and 1 μg/mL. Evaluations of cell morphology were performed on days 1, 3, 7 and 14. Cellular viability was evaluated on days 1, 3, 5 an

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Kim, Jin-Hyeong, Minji Kim, Somyeong Hwa, Youngkyung Ko, and Jun-Beom Park. "Vitamin E Enhances Cell Viability and the Osteogenic Differentiation of Cell Spheroids Made of Gingiva-Derived Stem Cells." Medicina 59, no. 4 (2023): 736. http://dx.doi.org/10.3390/medicina59040736.

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Background and Objectives: Vitamin E is reported to expedite new bone formation in animal models, and this has led to a decrease in the time needed for treatment. In this study, human gingiva-derived stem cell-derived spheroids were examined to determine the effects of vitamin E on cell survival, osteogenic differentiation, and mineralization. Materials and Methods: Human gingiva-derived stem cells were used to create spheroids, which were then cultivated with vitamin E at doses of 0, 0.1, 1, 10, and 100 ng/mL. The morphological examination and the qualitative and quantitative vitality of the

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Lee, W. J., G. H. Maeng, R. H. Jeon, G. J. Rho, and S. L. Lee. "210 COMPARATIVE CHARACTERIZATION OF MESENCHYMAL STEM CELLS DERIVED FROM MINIATURE PIG SYNOVIUM, SYNOVIAL FLUID AND BONE MARROW." Reproduction, Fertility and Development 24, no. 1 (2012): 217. http://dx.doi.org/10.1071/rdv24n1ab210.

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Mesenchymal stem cells (MSC) are a valuable cell source for cartilage tissue engineering because MSC derived from various tissues readily differentiate into skeletal cell and chondrogenic lineages. In this study, we compared the cellular characteristics of synovium (SN) and synovial fluid (SF)-derived MSC with bone marrow (BM)-derived MSC of miniature pig. Biopsies of SN and BM were collected and SF was flushed with saline solution from 6-month-old miniature pig (T-type, PWG Micro-pig, PWG Genetics Korea, South Korea). Cells were isolated from collected tissues and cultured in advanced-DMEM su

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