Dissertations / Theses on the topic 'Aloe extract'

CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior<br>The oral mucosa is one of the human body areas most frequently traumatized and usually presents traumatic ulcers. There are different treatment choices among which both professionals and patients may follow in order to treat oral ulcerations, for instance, formulations from plants extract among other natural products. The use of phytotherapics has a long history of success, however the associated use of substances still has poor scientific background. The aroeira-do-sertÃo (Myracrondruon urundeva AllemÃo) and Aloe vera (Aloe barbadensis Miller) have anti-ulcerogenic and anti-inflammatory proven activity when used as isolated substances and have been largely prescribed by health care professionals. The goal of this study was to evaluate the combined use of the aqueous extract of aroeira (10%) with the dry extract of aloe vera (40%) on the treatment of traumatic ulcers, as well as its toxicity. Traumatic ulcers were surgically created on the oral mucosa of Wistar rats and further exposed to the test solution containing both aroeira e aloe vera, twice a day. The animals from test and control grups were sacrificed by euthanasia after 01, 05 and 10 days. The oral ulcers were measured and histologically evaluated. Their corporeal mass were compared and a hematological analysis performed. Stomach, kidneys, liver and spleen were removed and microscopically investigated for signs of toxicity. According to the results, the combined solution of aroeira and aloe vera did not present systemic toxicity, neither had any influence on the ulcers healing performance, despite having reduced its initial growth.<br>A mucosa bucal à uma das Ãreas mais traumatizadas do organismo, podendo apresentar Ãlceras traumÃticas com frequÃncia. SÃo muitos os diferentes tipos de tratamento aos quais ambos profissionais e pacientes recorrem para tratar as lesÃes ulceradas, como por exemplo, as formulaÃÃes à base de extratos de plantas e produtos naturais. A utilizaÃÃo de fitoterÃpicos tem uma longa histÃria de sucessos, porÃm, o uso combinado de substÃncias ainda apresenta escassa referÃncia cientÃfica. A aroeira-do-sertÃo (Myracrodruon urundeuva AllemÃo) e a Aloe vera (Aloe barbadensis Miller) tÃm comprovada aÃÃo antiulcerogÃnica e anti-inflamatÃria quando usados isoladamente e sÃo amplamente indicados por profissionais da saÃde. O objetivo desse estudo foi avaliar a aÃÃo conjugada do extrato aquoso de aroeira (10%) e extrato seco de Aloe vera (40%) no tratamento de Ãlceras traumÃticas e sua toxicidade. Ãlceras traumÃticas foram realizadas cirurgicamente em mucosa de ratos Wistar e expostas a uma soluÃÃo teste contendo aroeira e aloe, aplicadas duas vezes ao dia. Os animais dos grupos controle e teste sofreram eutanÃsia em 01, 05 e 10 dias e tiveram suas Ãlceras medidas e avaliadas histopatologicamente. Foi tambÃm realizada uma comparaÃÃo da massa corpÃrea e uma anÃlise hematolÃgica em todos os animais. EstÃmago, rins, fÃgado e baÃo foram analisados por microscopia Ãtica para avaliaÃÃo de parÃmetros de toxicidade. De acordo com os resultados, a soluÃÃo conjugada dos dois fitoterÃpicos nÃo apresentou toxicidade sistÃmica, nem influenciou na cicatrizaÃÃo das Ãlceras, apesar de ter reduzido o seu crescimento inicial.

Aloe mites are herbivores of the genus Aloe (ALOACEAE) and are associated with hyperplastic growth in various aloe species, but the biochemistry of this interaction is poorly understood. In an effort to characterize plant defense responses to herbivory in the genus Aloe, a salivary extract was isolated from aloe mites (Aceria aloinis Keifer) and its bioactivity was tested using a hypocotyl elongation assay. Subsequently, Aloe striata plants were treated with jasmonic acid (JA), salicylic acid (SA), and the mite salivary extract. Using water and methanol, compounds of different polarity were extracted from aloe tissues that had been frozen and crushed at 4, 12, and 24h after treatment. Extracts were analyzed by HPLC and three compounds were found. One of these compounds was SA (mean concentration of 4µg/mL), and this is the first time that this aloe species has been found to produce SA. Two additional peaks of unknown identity were observed in JA- and SA-treated plants. These results suggest that A. striata may in fact undergo a JA-mediated change in secondary metabolism as part of a plant defense response.

Magister Scientiae - MSc (Chemistry)<br>Global climate change and geographical differences are two major parameters known to have, either, direct or indirect influence on the production of secondary metabolites in plants, which in-turn may affect the quality and/or quantity of the overall metabolites. The primary purpose of this thesis was to evaluate the phytochemistry of the whole leaf of Aloe succotrina Lam. spp. - a South African native plant - through a chromatographic spectroscopic approach-against available data accumulated for the cultivated population. Preliminary screening of the crude extracts i.e. HEX, DCM and EtOAc on TLC aluminium plates precoated with silica gel 60 F254 followed by various chromatographic separation, led to the isolation of five known compounds: ?-sitosterol (1) and two anthrone-C-glycosides (2 and 5), including two coumarin derivatives-the aglycone (3) and glycoside derivative (4). Notably, apart from 5 and 2, the accumulation of 1, 3 and 4 in A. succotrina Lam. spp. is reported for the first time. Structural elucidation of the individual compounds was achieved by extensive spectroscopic analysis i.e. MS, IR, 1H and 13C-NMR spectroscopy and in some cases comparison to the literature. A comparative HPLC chromatogram of the crude MeOH extract of the leaves of A. succotrina Lam. was developed for qualitative (and quantitative) identification of the active metabolites, which could be realized by VWD with detection at 290 nm.

Made available in DSpace on 2016-08-10T10:54:46Z (GMT). No. of bitstreams: 1 PATRICIA LIMA MERCES.pdf: 1291852 bytes, checksum: 8680fab655ce1b8b3f1410b572872b4c (MD5) Previous issue date: 2015-05-14<br>Objective: To evaluate the influence of Aloe vera (Aloe barbadensis) in angiogenesis and scar activity Methods: experimental study used 120 fertile eggs of chicken (Gallus domesticus) of Rhoss lineage. The eggs were incubated, and the end of the 13 °, filter paper discs, conveying 3 uL of the solution to be tested (glycolic extract of aloe vera 10%) and the proper controls were deposited directly on the Chorioallantoic Membrane-MCA so carefully to evaluate the angiogenic activity of Aloe vera. The healing activity was evaluated using 15 healthy rats of the species Rattus norvegicus albinus by creating a lesion on the dorsum of each mouse using a rectangular size 2,0x3,0cm mold, after making the injuries were applied the solution to be tested, Aloe vera and the positive and negative controls Results: regarding angiogenic activity observed If through the images that 80% of MCAs which was used Aloe vera was the formation of thicker blood vessels and in greater quantities, but there was no difference significant when compared to the positive control. Regarding scar activity results in macroscopic evaluation showed that 100% of the test group mice showed a complete closure of the lesions on the 21st day of the experiment while only 40% of the control group had complete closure. But when the Tukey test (p> 0.05), there was no statistical difference. Demonstrating how the scar activity of Aloe vera is the same as Regederm® you already have scientific evidence in wound healing. Conclusion: Aloe vera presented healing activity equal to the positive control, the MCA presented angiogenic activity equal to the positive control, with formation of new vessels, it was easy to apply and handling, conclusion themselves with what the Aloe vera extract or pro components -angiogênicos isolates may have potential pharmaceutical applications for wound treatment.<br>Objetivo: Avaliar a influência do Aloe vera (Aloe barbadensis) na atividade angiogênica e cicatricial Métodos: estudo experimental onde se utilizou 120 ovos férteis de galinha (Gallus domesticus) da linhagem Rhoss. Os ovos foram incubados e ao final do 13°, discos de papel de filtro, veiculando 3 µL da solução a ser testada (extrato glicólico de aloe vera a 10%), e os devidos controles, foram depositadas diretamente sobre a Membrana Corioalantóide-MCA de forma cuidadosa para avaliação da atividade angiogênica do Aloe vera. A atividade cicatricial foi avaliada utilizando 15 ratos saudáveis, da espécie Rattus norvegicus albinus, através da criação de uma lesão no dorsos de cada rato utilizando um molde do tamanho retangular 2,0x3,0cm, após a confecção da lesão eram aplicadas a solução a ser testada, Aloe vera e os controles positivo e negativo Resultados: com relação a atividade angiogênica observou se através das imagens que 80% das MCAs onde se utilizou o Aloe vera houve formação de vasos sanguíneos mais grossos e em maior quantidade, porém não apresentou diferenças significativas quando comparada ao controle positivo. Com relação a atividade cicatricial os resultados na avaliação macroscópica demonstraram que 100% dos ratos do grupo teste apresentaram um fechamento completo das lesões no 21º dia do experimento enquanto que apenas 40% do grupo controle apresentavam fechamento completo. Porém quando aplicado o teste de Tukey, (p>0,05), não houve diferença estatística. Demostrando assim que a atividade cicatricial do Aloe vera é igual à do Regederm® que já possui comprovação cientifica na cicatrização de feridas. Conclusão: o Aloe vera apresentou atividade cicatrização igual ao controle positivo, na MCA apresentou atividade angiogênica igual ao controle positivo, com formação de novos vasos, foi de fácil aplicação e manuseio, Concluindo -se com isso que o extrato de Aloe vera ou componentes pró-angiogênicos isolados podem ter potencial para aplicações farmacêuticas para o tratamento de feridas.

Se evaluó el efecto antiinflamatorio y cicatrizante del Gel del extracto de Aloe vera y su contenido (sábila) procedente de Lima, se preparó el Gel del extracto de Aloe vera al 20% que fue aplicado por vía tópica en 40 pacientes entre las edades 20 a 50 años, de ambos sexos, en el Establecimiento de Salud Ganimedes DISA LIMA ESTE del Ministerio de Salud, y otro grupo de 40 pacientes (grupo control) sin la aplicación del Gel del extracto de Aloe vera, comparando ambos casos se demostró la eficacia antiinflamatoria y cicatrizante del Gel del extracto de Aloe vera, que consistió en el control de la medición de la inflamación y la herida leve cerrada al paciente, desde su llegada por Emergencia (tópico) al establecimiento de Salud, por efecto de un trauma accidental o por otra índole; primero se evalúo el estado general del paciente para un diagnóstico médico, luego se plantea para su consentimiento del estudio a realizar, iniciando el uso tópico mediante controles de observación y medición de la zona inflamada y herida leve cerrada, así como el cambio de color de la piel y reacciones adversas que puede ocasionar el uso tópico hasta su recuperación total. El estudio del Gel del extracto de Aloe vera (sábila) de acuerdo con los ensayos efectuados y los objetivos generales propuestos, han demostrado su efecto anti inflamatorio y cicatrizante en la parte externa de la piel, el cual se realizó por el método mecánico y tópico. El Gel del extracto de Aloe vera, es un producto natural muy económico, de acceso fácil en su elaboración, presentando actividad antiinflamatoria y cicatrizante, administrada por vía tópica en los pacientes tratados en mención. La calidad de la inflamación y la cicatrización obtenida con Gel del extracto de Aloe vera demuestra que es de gran utilidad en tratamiento dermatológico o en cirugía plástica, obteniendo mejores resultados en regeneración de tejido o piel y epitelización de las heridas. Palabras clave:Gel del extracto de Aloe vera, efecto: antiinflamatorio y cicatrizante, Dermatológico.<br>The effect of anti-inflammatory and healing gel of Aloe vera and its contents (Aloe vera) from Lima, was prepared Gel Aloe vera extract 20% was applied topically to 40 patients aged 20 to 50, of both sexes, in the health facility Ganymede DISA LIMA EAST Ministry of Health, and another group of 40 patients (control group) without the application Gel of Aloe vera, comparing both anti-inflammatory efficacy was demonstrated Scar Gel and Aloe vera extract, which consisted medicine control inflammation and mild closed injury to the patient, since his arrival in Emergency (topical) the establishment of Health, due to accidental trauma or other nature, first performed an overall patient for a medical diagnosis, then posed for consent to conduct the study, starting with the topical use of observation and measurement controls the inflamed area and minor wound closed, and the change of color of the skin and can cause adverse reactions to topical total. The recovery study Gel Aloe vera (Aloe) according to those tests and the general objectives proposed have demonstrated its anti inflammatory and healing on the outside of the skin, which is performed by the mechanical method and topical. The gel of Aloe vera is a natural product very economical, easy to access in their development, presenting antiinflammatory and healing, administered topically in patients treated in mention. The inflammation and scarring gel obtained Aloe vera extract shows that it is of great use in treating dermatological or plastic surgery, obtaining better results in tissue regeneration or epithelialization of skin wounds. Key words: Gel of Aloe vera Extract, effect: anti-inflammatory and healing, Dermatology.<br>Tesis

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Química, Florianópolis, 2016.<br>Made available in DSpace on 2017-06-27T04:08:43Z (GMT). No. of bitstreams: 1 346010.pdf: 2085302 bytes, checksum: 99549ec52928f201e606c2cf4121f97b (MD5) Previous issue date: 2016<br>Diversas culturas se baseiam na utilização de plantas para o tratamento de doenças. Plantas do gênero Aloe vem sendo utilizadas a séculos na medicina tradicional para cicatrização de feridas, como agente antibacteriano, como laxante e até mesmo para o tratamento do câncer. O extrato do parênquima clorofiliano (EPC) da Aloe barbadensis Miller é excretado quando a folha é cortada ou quebrada, este líquido de cor amarelada é rico em antronas, cromonas e seus derivados. Estudos com compostos isolados do EPC, aloína e Aloe emodina, mostram uma promissora atividade antitumoral dos mesmos. A incidência do melanoma cutâneo vem aumentando nos últimos anos, o que gera uma grande preocupação, uma vez que esse câncer é altamente agressivo apresentando alta possibilidade de metástase. Neste trabalho o EPC foi padronizado por HPLC, apresentando 5,6% em massa seca do isômero aloína B. A citotoxicidade foi avaliada do EPC em linhagens de fibroblastos L-929, assim como o efeito do EPC sobre a viabilidade, proliferação, morfologia e migração de duas linhagens de melanoma (SK-MEL-28 e B16-F10). De acordo com a ISO 10993-5, concentrações superiores a 185 µg·mL-1 de EPC são citotóxicas (decréscimo na viabilidade maior que 30%), sendo esse valor maior que o valor de IC50 de ambas as linhagens de melanoma, 125 e 165 µg·mL-1 para a SK-MEL-28 e B16-F10, respectivamente. Análise morfológica mostrou ponto de alta fluorescência, indicando uma reorganização dos filamentos de actina presentes no citoesqueleto. O resultado do ensaio de migração para ambas linhagens de melanoma mostrou que o EPC é mais eficaz em inibir a migração do melanoma humano SK-MEL-28. Células endoteliais (HUVEC) foram expostas ao EPC e os parâmetros de viabilidade celular, proliferação, morfologia, migração e formação de tubos foram avaliados. O EPC inibiu a migração de HUVECs mesmo em concentrações que não apresentaram efeito de decréscimo na atividade metabólica e proliferação celular. Pôde-se observar a retração das fibras de estresse em células tratadas com as concentrações de 10 e 100 µg·mL-1 de EPC. A formação de tubos em células endoteliais foi inibida na maior concentração testada (100 µg·mL-1), contudo ainda se observa a formação de brotos vasculares. Em suma o EPC proveniente de Aloe barbadensis Miller causou um decréscimo na viabilidade e proliferação celular assim como uma diminuição da capacidade de migração e alterações morfológicas de células de melanoma e endoteliais, influenciando também na formação de tubos vasculares por células endoteliais.<br>Abstract : Several cultures are based on the use of plants for the treatment of diseases. Plants of the genus Aloe has been used for centuries in traditional medicine, in wound healing, such as antibacterial agent, laxative and even for the treatment of cancer. The chlorophyll parenchyma extract (EPC) from Aloe barbadensis Miller is excreted when the sheet is cut or broken, this yellowish liquid is rich in anthrones, chromones and their derivatives. Studies on compounds isolated EPC, aloin and Aloe emodin, showed promising antitumor activity. The incidence of melanoma has increased in recent years, creating a great concern, since this cancer is highly aggressive and presents high possibility of metastasis. In this work the EPC was standardized by HPLC, showing 5.6% of dry weight of aloin B isomer. The EPC cytotoxicity was evaluated on fibroblast L-929 cell line, as well as the effect of EPC in cell viability, proliferation, morphology alterations and migration on two melanoma cell lines (SK-MEL-28 and B16-F10). According to ISO 10993-5, concentrations higher than 185 µg·mL-1 EPC are cytotoxic (decrease in viability was greater than 30%), this value being higher than the IC50 value of both melanoma cell lines, 125 and 165 µg·mL- 1 to SK-MEL-28 and B16-F10, respectively. Morphological analysis showed high fluorescence spots, indicating a reorganization of actin filaments present in the cytoskeleton. The result of the migration test for both melanoma cell lines showed that the EPC is more effective to inhibit the migration of human melanoma SK-MEL-28. Endothelial cells (HUVEC) were exposed to the EPC and parameters of cell viability, proliferation, morphology alterations, migration and tube formation was evaluated. The EPC inhibited HUVEC migration even at concentrations that has not shown effect in decrease of metabolic activity and cellular proliferation. It was observed the decrease of stress fibers in cells after treated with concentrations of 10 and 100 µg·mL-1of EPC. The tube formation on endothelial cells was inhibited at the highest concentration tested (100 µg·mL-1), however it was still possible to observe the formation of vascular sprouts. In short, the EPC from Aloe barbadensis Miller caused a decrease in cell viability and proliferation as well as reduced migration ability and morphological changes on melanoma and endothelial cells, also influencing the vascular tube formation by endothelial cells.

Los esfuerzos en investigación científica están dirigidos en la búsqueda de nuevos tratamientos que puedan minimizar el deterioro por corrosión de los materiales metálicos, ya que es un problema que en el sector industrial viene presentándose en los últimos años, la corrosión tiene un impacto en altos costos en mantenimiento y reparación industrial en el que involucra el uso de aceros como el AISI-1045 grado estructural, el acero 4140 grado herramienta y el acero AISI-8620 grado maquinaria, estos aceros se desempeñan en ambientes agresivos durante su servicio. Ante estos problemas y la necesidad de proteger los aceros en la industria para un mejor aprovechamiento de su vida útil, se utilizaron 2 inhibidores ecológicos biodegradables capaces de inhibir el deterioro químico de los materiales metálicos los cuales fueron el GEL de Aloe-Vera y el extracto etanólico de Morinda Citrifolia.<br>It is well known that metallic materials could be corroded during their service due to their exposed environment, which is considered as one of the main problems in the manufacture industry that has been occurred in the last century. Corrosion can cause from loss of human lives until spill of economic, in addition to failure in electrical power plants, serious disasters on bridges, collapsing concrete buildings, immediate deterioration of ships, leaking pipelines, mechanical damage failure, surface alteration properties, another sever damages in infrastructure, in addition to this damage, corrosion also causes irreversible impact on the environment as air or soil pollution and global warming. In order to reduce the damage caused by corrosion mechanism, different kind of methods, procedures and/or treatments have been developed, among which the use of corrosion inhibitors (IC), where a classification as organic inhibitor has been existed, this creates a stack of molecules to form multi-layer between the metal material and the corrosive media. Therefore, in this research the efficiency of inhibition (EI) of two biodegradable natural liquid-extracts is evaluated in an aqueous solution of hydrochloric acid (HCl) 1 Molar, also IC molecules are considered as a new alternative capable of inhibiting the chemical deterioration of any metallic material and consequently minimize the corrosion problems above describe. The liquid inhibitors studied here were; extracts from Morinda Citrifolia leaves (ethanol extracted) and GEL (aqueous extracted) leaves of Aloe-Vera, commonly known as Sabila. These liquid-extracts were added at different volume ratios to acid (HCl-1M) media. Their performance as corrosion inhibitor is then monitored and evaluated by electrochemical techniques as polarization TAFEL (Ecorr vs. I) and spectroscopy Impedance electrochemical (EIS, Zreal vs. Zimag). From EIS parameters, the EIS data were analyzed by a correlation with an appropriate equivalent electric circuit (EEC) and the TAFEL data were used to determined EI%, whereas the thermodynamic analysis by using the adsorption isotherms the type of inhibitor was classified as mixed. Additionally, Optical Microscopy images were obtained before and after undergoing industrial steels in contact with the corrosive solution without extracts from the leaves of Morinda Citrifolia and Aloe-Vera that serve as inhibitors. On the other hand, properties such as contact angle, viscosity and surface tension of the natural organic molecules were obtained in order to determine the wettability and the mechanism of adsorption of organic molecules (natural liquid extracts) with the surface of the steels exposed to corrosive environment. According to the EIS results of industrial steels (AISI 1045, 4140 and 8620) studied here, they had pitting corrosion after hours exposure in HCl-1M. Meanwhile, the use of GEL extracted from Aloe-Vera (Aloe) demonstrated to be corrosion inhibitor efficiencies, EI~ 75% at 10 ml to 1045 steel, 4140 steel EI~ 70% at 16 ml and 8620 for steel EI~ 30% from 2ml. Meanwhile the ethanol extract of Morinda Citrifolia proved to be an excellent inhibitor capable to minimize the pitting corrosion process from 4 or 6 mL of its volume concentration (steel 1045, EI> 93.1%, steel 4140 and 8620 EI> 92.9%). This difference in inhibition efficiency relates to the contact angle and the surface tension of the drop of Morinda Citrifolia or Aloe-Vera, in which the contact angle results greater and better adsorption to extracted of Morinda Citrifolia.

Introducción: El acemanano presente en las hojas de Aloe vera, es un polisacárido que posee una gran actividad en la regeneración celular y modulación del sistema inmune. Objetivo: Determinar el efecto regenerador óseo del extracto acuoso liofilizado de hojas de Aloe vera (Sábila) sobre la osteotomía mandibular inducida en cobayo. Diseño: Experimental. Lugar: Laboratorio de Farmacología, Facultad de Medicina Humana - UNMSM. Intervenciones: Se utilizó 40 cobayos machos con un peso corporal 1000±120g, se formó 5 grupos aleatoriamente de 8 animales; grupo I: Suero fisiológico al 0,9% (2mL/kg), grupo II: alendronato (0.5mg/kg), los grupos III, IV y V: extracto acuoso liofilizado de hojas de Aloe vera a dosis de 50mg/kg, 250mg/kg y 500mg/kg, respectivamente. Los cobayos fueron sometidos a osteotomía mandibular unilateral. Principales medidas de resultados: Recuento de células por campo, niveles de fosfatasa alcalina. Resultados: Se evidencia incremento significativo de fosfatasa alcalina, osteoclastos y osteocitos (p<0,05) a los 15 y 30 días en los grupos que recibieron alendronato y Aloe vera; también aumento de fibroblastos a los 15 días en los grupos III y IV; no se encontró diferencia significativa (p>0,05) en el recuento de linfocitos ni osteoblastos a los 15 y 30 días. Conclusiones: El extracto acuoso liofilizado de Aloe vera posee efecto regenerador óseo. Palabras clave: Regeneración ósea, Aloe vera, alendronato<br>--- Introduction: The acemannan present in the leaves of Aloe vera, is a polysaccharide having a high activity in cell regeneration and modulating the immune system. Objetive: To determine bone regenerative effect of the lyophilized aqueous extract of leaves of Aloe vera on the mandibular osteotomy induced in guinea pigs. Design: Experimental. Location: Laboratory of Pharmacology, Faculty of Human Medicine - UNMSM. Interventions: 40 male guinea pigs was used with a 1000±120g body weight, 5 groups of 8 animals randomly formed; Group I: 0.9% physiological saline (2mL/kg), group II: Alendronate (0.5mg/kg), the groups III, IV and V: lyophilized aqueous extract of Aloe vera leaves at doses of 50mg/kg, 250mg/kg and 500mg/kg respectively. The guinea pigs were undergoing unilateral mandibular osteotomy. Main outcome measures: Count cells per field, and alkaline phosphatase levels. Results: Significant increase in alkaline phosphatase, osteoclasts and osteocytes (p <0.05) at 15 and 30 days in the groups receiving alendronate and Aloe vera is evident; fibroblasts also increased to 15 days in groups III and IV; no significant difference (p> 0.05) was found in the lymphocyte count or osteoblasts at 15 and 30 days. Conclusions: The dried aqueous extract of Aloe vera has bone regenerative effect. Keywords: Bone regeneration, Aloe vera, alendronate<br>Tesis

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia Química.<br>Made available in DSpace on 2012-10-23T18:41:08Z (GMT). No. of bitstreams: 1 255526.pdf: 567231 bytes, checksum: 6583371d78d840b65262cca92aed1651 (MD5)<br>Aloe barbadensis, uma espécie originária do continente africano adaptada no Brasil, possui diversas propriedades medicinais determinadas por metabólitos encontrados nos extratos dos parênquimas de reserva e clorofiliano. Este último é rico em antraquinonas, sendo constituído majoritariamente de aloína. No presente trabalho, foram estudados os efeitos do extrato do parênquima clorofilano (EPC) e da aloína sobre a viabilidade de células tumorais da linhagem B16F10 in vitro e a formação de vasos sangüíneos de embriões de Gallus domesticus in vivo. Na análise in vitro, células incubadas a 37ºC em atmosfera contendo 5% de CO2, foram tratadas em diferentes concentrações de aloína e de EPC. Tanto a aloína (0,01 a 230 µg?mL-1) como o EPC (0,01 a 100 µg?mL-1) reduziram a viabilidade das células tumorais. Os dados experimentais foram representados através de um modelo matemático, evidenciando que o EPC mostrou-se 2,3 vezes mais citotóxico para as células tumorais do que a aloína. Ao contrário do verificado sobre a atividade antitumoral, a aloína (10 a 320 µg?mL-1) e o EPC (10 a 320 µg?mL-1) estimularam de modo dose-dependente a vasculo/angiogênese na membrana vitelínica (MV) em 154 e 177% e a angiogênese na membrana corioalantóica (MC) em até 131 e 164%, respectivamente. Verificou-se ainda que o efeito da aloína e do EPC superaram o efeito do fator angiogênico FGFb na formação de vasos primordiais na MV (135%), sendo que para a angiogênese, na MC, apenas o EPC foi mais efetivo que o FGFb (164% versus 158%). Os tratamentos não modificaram o padrão de crescimento e a morfogênese dos embriões, com base nas medidas de comprimento total e percentual de comprimento cefálico de embriões com quatro dias de desenvolvimento. Os resultados demonstram que a aloína e o EPC exerceram efeito citotóxico especificamente nas células tumorais, mas promoveram atividade pró-vasculogênica e pró-angiogênica, superior a do fator angiogênico FGFb, sem qualquer evidência de embriotoxicidade. Com base nos resultados é possível concluir que o EPC e a substância isolada, sob estudo, mostraram concomitantemente um potencial efeito antitumoral e uma atividade estimulante em processos fisiológicos como a vascularização de tecidos normais.

La humanidad y los materiales continúan evolucionando a un ritmo similar, ya que, con el trascurso del tiempo, el desarrollo de estos se ha modificado con respecto a las necesidades y demandas de la humanidad. Anteriormente, la creación de materiales y herramientas estaba limitada por la disponibilidad de materia prima en el entorno; y ahora es posible definir las características del material deseado, previo a su producción. No obstante, la producción de materiales sigue dependiendo de los recursos naturales. Por tal motivo, es necesario considerar el desarrollo e investigación en materiales y tecnologías que no acrecienten el impacto sobre el medio ambiente y la naturaleza, pues de ello depende nuestro futuro y supervivencia. Actualmente se realiza investigación para desarrollar materiales capaces de disminuir la inestabilidad física o química de compuestos de interés, pues dichos materiales son altamente demandados en la industria, debido a la necesidad de otorgar estabilidad y protección a las moléculas o compuestos de interés. Una de las alternativas más atractivas para dar solución al problema de inestabilidad es la encapsulación, la cual consiste en el recubrimiento de compuestos o moléculas, mediante un agente encapsulante que, en la mayoría de los casos es de naturaleza polimérica. Con base en lo anterior, el objetivo del presente trabajo consistió en evaluar la adición de Aloe arborescens a un agente encapsulante base ALG y PEG como preservador de extracto de Clitoria ternatea, mediante los datos obtenidos en: la eficiencia de encapsulación (%EE), el rendimiento en masa (%Rm), la cinética de liberación (%CL) y el punto de flujo (G’=G”) de cada una de las formulaciones del agente encapsulante. Los datos obtenidos se sometieron a un análisis estadístico y se concluyó que, la adición de biomasa de Aloe arborescens tiene un afecto estadísticamente significativo sobre la eficiencia de encapsulación y el punto de flujo, aunque, no se reflejó algún efecto estadísticamente significativo en el rendimiento de masa y la cinética de liberación.<br>CONSEJO MEXIQUENSE SE CIENCIA Y TECNOLOGÍA: Beca de titulación. UAEM/4561/2018/CIV: Beca de investigación

Honey and plant extracts have a long hitory of medicinal use in Sudan that continues to the present day. The antimicrobial properties of Manuka honey and its use as a topical wound treatment is now widely recognised but of limited availability in developing countries. This study examines the physico-chemical properties and antimicrobial activity of Sudanese honeys and synergistic interactions between selected honeys and plant extracts. A sample of 60 floral honeys from Sudan were collected direct from honey producers and 15 plant extracts were obtained from Sudan National Centre for Research. These were characterised and the antimicrobial activity against Escherchia coli (NCTC 10418) and Staphylococcus aureus (NCTC 6571) determined using bioassay and minimum inhibitory concentration methods. Selected honeys, Ethylenediaminetetractic acid (EDTA) and plant extracts were then studied for synergistic interactions. Although the honeys had similar physico-chemical characteristics as honeys from other published studies, none exhibited the non-peroxidal activity associated with Manuka honey. A number exhibited strong peroxidal activity. The type of hive used, the site of collection and producer (beekeeper) had a statistically significant impact on the antimicrobial activity. Acacia nioltica and Tamarindus indica plant extracts showed marked antimicrobial activity before and after autoclaving and were chosen for synergistic interaction with a selected honey. Synergy was screened using an agar diffusion bioassay using Canvas software to measure the change in area of zone of growth inhibition to determine bacteriostatic synergy. This was then confirmed using the time-kill curve method. Synergistic interaction was noted between honey and EDTA against Staphylococcus aureus and Acacia and EDTA against Escherichia coli. The results are discussed in relation to the medical use of honey and plant extracts in Sudan and concludes that there is a need to further explore the potential for local production of bioactive honeys for use in conjunction with plant extracts. The Sudanese government should conduct further translational research in this area.

Healthcare-associated Infections (HAIs) in Europe account for around 16 million additional patient-days in hospital per year (an average of 4 days per infection), with around 37,000 deaths associated with the infection itself .The total estimated annual cost of hospital-acquired infections for Europe is 7 million euros (ECDC, 2008). Surgical Site Infections (SSIs) account for between 15% and 25% of all HAIs, ranking third in order of frequency (Bozic et al, 2005). SSIs resulting from joint replacement operations are serious complications both from the patient’s and from an economic point of view. The onset of an SSI is associated with increased morbidity and mortality rates and thus leads to added costs for the National Health Service in terms of prolonged hospital stays (De Lissovoy et al, 2009), related diagnostic procedures and possible revision surgery (Perencevich et al , 2003). It is estimated that the onset of an SSI following orthopaedic surgery doubles the risk of re-admissionsin the following 12 months and that the direct costs of hospitalisation account for more than double ( Broex et al 2009). The cost of revision surgery for an infected hip replacement is 2.8 times higher than a non-septic revision and 4.8 times higher than the original implant (Bozic et al, 2005). The aim of this study is to develop a model for analysing healthcare expenditure borne as a result of the occurrence of Surgical Site Infections (SSIs) in patients undergoing hip and knee replacement surgery. The study was conducted at the Gaetano Pini Orthopaedic Institute. Selection of the patients took place within the framework of the ISChIA project on active prospective surveillance among patients who had experienced a SSIs. This aim was achieved by developing a model (Phase I) for evaluating the impact of SSIs – as defined by the CDC – on healthcare expenditure, which considers both the perspective of the hospital providing the treatment and that of the Regional Health Service. Direct extra costs were analysed from the perspective of the healthcare provider, using bottom-up and micro-costing methods; these estimated costs include the direct variable costs (resources used for specific patient services) of treating the infection borne within the hospital, including treatment costs, laboratory costs, diagnostic examinations, x-rays and consultation costs Given a case of hospitalisation with SSI, the hospital meets the direct costs arising therefrom, which would otherwise not have been generated. At the same time, the Regional Health Service, in addition to these costs, must meet any costs relating to new cases of patients being admitted as due to SSI. The model thus implemented was then subject to validation during Phase II. The average additional value estimated for managing SSI is €4,905 in Phase I and €4,155 in Phase II. ECDC. Focus on healthcare-associated infections, Annual epidemiological report 2008. De Lissovoy, G.; Fraeman, K.; Hutchins, V. et al. ‘Surgical Site Infection: Incidence and Impact on Hospital Utilization and Treatment Costs’, American Journal of Infection Control. 2009; 37(5):387-97. Perencevich, E.N.; Sands, K.E.; Cosgrove, S.E. et al. ‘Health and Economic Impact of Surgical Site Infections Diagnosed after Hospital Discharge’, Emerging Infectious Diseases. 2003; 9(2):196-203. Broex, E.; Van Asselt, A.; Bruggeman, C.A.; Van Tiel, F.H. ‘Surgical site infections: how high are the costs?’, The Journal of Hospital Infection. 2009; 72(3):193-201. Bozic, K.J.; Ries, M.D. ‘The impact of infection after total hip arthroplasty on hospital and surgeon resource utilization’, J Bone Joint Surg Am. 2005 Aug; 87(8):1746-51

The high prevalence of metabolic syndrome in children is mainly due to the adoption of an unhealthy diet and a sedentary lifestyle. The disorder is also associated with obesity, insulin resistance, hypertension and increased blood levels of triglycerides, free fatty acids and glucose. Although metabolic syndrome and its metabolic complications can be managed by the use of conventional medicines such as fenofibrate and metformin, there is a growing use of plants with medicinal properties. Aloe vera has been used for several medicinal purposes such as wound and burn healing, treatment of diabetes and treatment of cancer. Aloe vera also has biological activities that include anti-inflammatory, anti-microbial and laxative effects. Previous studies exploring the metabolic effects of Aloe vera have been in adult animals where metabolic syndrome was induced by the use of pharmacological agents. However, its beneficial effects in growing children who are at risk of developing metabolic syndrome have not been fully explored. The objectives of this study were to evaluate the effects of a crude Aloe vera leaf extract on circulating metabolic substrates, the morphometry and morphology of the gastrointestinal tract and the liver function of growing rats. Fifty-nine male Sprague-Dawley rats of 21 days old were randomly divided into one of six treatment groups. Group I (control) was fed normal rat chow (NRC) with plain gelatine cubes (vehicle). Group II received a high carbohydrate diet (HCD) with plain gelatine cubes; Group III received normal rat chow and fenofibrate at 100mg.kg-1; Group IV received a HCD and fenofibrate at 100mg.kg-1; Group V received a normal rat chow and Aloe vera at 300mg.kg-1; Group VI received a HCD and Aloe vera at 300mg.kg-1. The Aloe vera and fenofibrate were suspended in gelatine cubes and administered daily. After 20 weeks of feeding, the rats were fasted over night and an oral glucose tolerance test (OGTT) was performed. The rats were then euthanazed after 48 hours of re-feeding and tissues were collectedfor further analysis. The data was expressed as mean ± SEM and analyzed by a one-way ANOVA. A repeated measures ANOVA was used for statistical analysis of the data from the oral glucose tolerance test. The values were considered statistically significant when p < 0.05 followed by a Bonferroni Post hoc test. After 20 weeks, the growing rats fed a high carbohydrate diet had a significantly higher body mass than the other groups (p < 0.05, ANOVA), however the administration of fenofibrate prevented the high carbohydrate-induced increase in body mass whilst Aloe vera was not effective. Linear growth as measured by the tibial length was not significantly different between the groups (p >0.05, ANOVA). There was no significant difference in the mass and relative density of the tibia bones of the rats between the groups. Feeding rats a HCD resulted in a higher (p< 0.05, ANOVA) visceral fat mass in the rats. Fenofibrate administration prevented the HCD-induced visceral fat mass gain whilst Aloe vera administration had no effect. Whilst the treatments did not result in any significant differences in the lengths and mass of the small intestine, the mass of the large intestine was significantly lower in the rats that received the HCD alone (p < 0.05, ANOVA). Fenofibrate administration resulted in a significantly increased liver mass compared to the other groups (p < 0.05, ANOVA). However there was no significant difference in the lipid and glycogen content in the liver. Fasting concentrations of metabolic substrates (glucose, triglycerides and free fatty acids) were not significantly different between the groups and no significant differences were observed in the circulating concentrations of insulin and the homeostasis model assessment of insulin resistance (HOMA-IR) (ANOVA; p>0.05). The OGTT did not show any abnormalities in the ability of the rats to handle a glucose load between the groups. An indirect assessment of liver function was performed by measurements of the blood concentrations of alkaline phosphatase (ALP), total bilirubin (TBIL), alkaline transaminase (ALT) and gamma-glutamyl transferase (GGT). There were significantly increased (p<0.05) ALP levels in rats fed NRC + FENO compared to the others. TBIL levels were significantly lower in rats fed a NRC + Av (p< 0.05, ANOVA) however the TBIL levels were within the normal range. The alkaline transaminase (ALT) levels were not significantly different between the groups (p < 0.05, ANOVA) and GGT was not detectable in any of the groups. Weaning rats onto a high carbohydrate diet and feeding them the diet for 20 weeks resulted in the development of visceral obesity without altering the glucose tolerance and metabolic substrates. The treatment with fenofibrate prevented the high carbohydrate diet-induced visceral adiposity however compared to fenofibrate, treatment with the Aloe vera leaf preparation was not significantly effective.

El Aloe vera (Aloe barbadensis Mill.) es una planta medicinal muy utilizada en todo el mundo desde el comienzo de la historia humana.<br>Este trabajo fue financiado por el Fondo Europeo de Desarrollo Regional (FEDER) a través del Programa Operacional de Competitividad e Internacionalización (POCI) y por fondos nacionales a través de la Fundación para la Ciencia y Tecnología (FCT, Portugal), en el marco del Proyecto POCI-01-0145-FEDER-030463: AllNat, y por el programa FEDER-Interreg España-Portugal, bajo el proyecto 0377_Iberphenol_6_E.

碩士<br>南華大學<br>自然醫學研究所<br>93<br>Antioxidants are reported to improve wound healing and protect tissues from oxidative injury. This study was designed to evaluate the in vitro antioxidant capacity of propolis, Lithospermun erythrorhison, Angelica sinensis, Aloe vera and formula using different antioxidant tests, including free radical scavenging, metal chelating activities, reducing power, total antioxidant activity, and lipid peroxidation. Experimental results indicated that the antioxidant activities increased with concentrations. Among all extracts, propolis, Lithospermun erythrorhison and formula had better antioxidant capacity of above in vitro antioxidant tests. Propolis and Lithospermun erythrorhison, contained the highest levels of total phenol contents and flavonoid contents, was the best inhibitor of lipid peroxidation and scavengers of DPPH, and ABTS radicals. In addition, propolis and Lithospermun erythrorhison had effective reducing power and metal chelating activities. Notably, this in vitro study of antioxidant effects demonstrated that antioxidant activities were correlated well with the contents of phenol or flavonoid compounds.

Mestrado em Engenharia Quimica IPB-ESTG<br>Aloe vera (Aloe barbadensis Mill.) is a succulent xerophytic medicinal plant that has assumed, in the last decades, great importance in the industrial sector due to its bioactive properties, which is why it is used in the development of cosmeceuticals, functional foods and food supplements. In this work, the focus is on the green rind of the Aloe vera leaf, which is usually considered a waste part of the plant. The main objectives were to obtain the partition coefficients of the main anthrones and chromones present in the extracts to support the design of fractionation processes using centrifugal partition chromatography (CPC), to develop a semi-solid formulation for topical use containing glycerol (G), propylene glycol (PG) and water (W) extracts, and characterize the bioactive properties and shelf stability of the final formulation. The chemical characterization of aloesin-rich extracts showed that the highest content of phenolic compounds was detected in W extract and the most abundant compounds were aloeresin B and aloin A. The G extract presented the best DPPH radical scavenging activity followed by W and PG extracts. The G and W extracts were the most stable when exposed to UVA and UVB radiation at different tested skin pH (3-6). Also, the three extracts had a MIC = 5 mg/mL against the bacterial pathogen of the skin Enterococcus faecalis, which can be beneficial for application in the cosmeceutical field. Antibacterial results showed that the PG extract presented broad spectrum antibacterial activity against Gram-positive and Gram-negative bacteria. PG and G extracts also presented anti-inflammatory activity. After incorporation of the extracts in a base cream, the final formulations presented higher antioxidant activity compared to the base cream (control). The antioxidant activity was evaluated at different storage condition along time and the results showed that the extracts retained its functionality (antioxidant capacity) after being incorporated in the base cream. The colour and pH analysis showed that the final formulations had good physicochemical stability when stored during 14 days at different temperatures. Finally, regarding the partition coefficients studies of the main anthrones and chromones present in the extracts, depending on the target compounds, very promising results were obtained using binary or ternary mixtures of the biphasic system water + butan-1-ol + ethyl acetate.<br>A Aloe vera (Aloe barbadensis Mill.) é uma planta medicinal xerofítica suculenta que tem assumido, nas últimas décadas, grande importância no setor industrial devido às suas propriedades bioativas, razão pela qual é utilizada no desenvolvimento de cosmecêuticos, alimentos funcionais e suplemento alimentares. Neste trabalho, o foco está na casca verde da folha de Aloe vera, que normalmente é considerada um resíduo da planta. Os principais objetivos foram obter os coeficientes de partição das principais antronas e cromonas presentes nos extratos para suportar o projeto de processos de fracionamento por cromatografia de partição centrífuga, desenvolver uma formulação semissólida para aplicação tópica contendo extratos de glicerol (G), propileno glicol (PG) e água (W), e caracterizar as propriedades bioativas e a estabilidade da formulação final. A caracterização química dos extratos ricos em aloesina mostrou que o maior teor de compostos fenólicos foi detetado no extrato W e os compostos mais abundantes foram aloeresina B e aloína A. O extrato G apresentou a melhor atividade sequestradora de radicais DPPH, seguido pelos extratos W e PG. Os extratos G e W foram os mais estáveis quando expostos à radiação UVA e UVB nos diferentes pH de pele testados (3-6). Além disso, os três extratos apresentaram CIM = 5 mg/mL contra o patógeno bacteriano da pele Enterococcus faecalis, o que poderá ser benéfico para aplicação na área cosmecêutica. Os resultados antibacterianos mostraram que o extrato de PG apresentou atividade antibacteriana de largo espectro contra bactérias Gram-positivas e Gram-negativas. Os extratos de PG e G também apresentaram atividade anti-inflamatória. Após incorporação dos extratos no creme base, as formulações finais apresentaram maior atividade antioxidante em relação ao creme base (controlo). A atividade antioxidante foi avaliada em diferentes condições de armazenamento ao longo do tempo e os resultados mostraram que os extratos retiveram a sua funcionalidade (capacidade antioxidante) após incorporação no creme base. As análises de cor e pH mostraram que as formulações finais apresentaram boa estabilidade físico-química quando armazenadas por 14 dias a diferentes temperaturas. Finalmente, em relação aos estudos dos coeficientes de partição das principais antronas e cromonas presentes nos extratos, obtiveram-se resultados muito promissores utilizando misturas binárias ou ternárias do sistema bifásico água + butan-1-ol + acetato de etilo, em função dos compostos alvo.

For centuries Aloe vera has been exploited for several verified and unverified medicinal uses such as wound healing, treatment of gastrointestinal ulcers and for its many biological effects including anti-microbial, laxative, anti-inflammatory and immunostimulatory activities. Studies have generally focused on its effects in vitro and in adults. When nursing mothers use Aloe vera extracts, their suckling infants are at risk of indirect exposure to Aloe vera via breast feeding or directly as dietary/health supplements. The gastrointestinal tract (GIT) of the neonate is sensitive to dietary manipulations during the suckling period with long lasting effects that can be irreversible. Thus babies may be at risk if administered Aloe vera extracts directly as dietary supplements or indirectly via breast milk. The main objectives of this study were to evaluate the effects of orally administered aqueous and alcohol extracts of Aloe vera on growth performance, the morphometry and morphology of the gastrointestinal tract and accessory organs, and liver function of suckling rats. Suckling Sprague-Dawley rats (77), males (n=38) and females (n=39) of 6 days old were randomly assigned to one of five treatment groups and given once daily by oral gavage a suspension of lyophilized crude alcohol or aqueous extracts of Aloe vera suspended in distilled water. Group I (control) was gavaged with distilled water (vehicle). Group II received a low dose of the aqueous extract (AqL) at 50mg. kg-1; Group III received a high dose of the aqueous extract (AqH) at 500mg. kg-1; Group IV received a low dose of the alcohol extract (AlcL) at 50mg. kg-1 whilst Group V received a high dose of the alcohol extract (AlcH) at 500mg. kg-1. The extracts and distilled water were 2 administered at a volume of 10ml.kg-1. The pups remained with their dams for the duration of the study and after 8 days on the treatments, the pups were humanely killed to harvest their tissues for measurements and physiological analysis. All data were expressed as mean ± SD and analyzed by one way ANOVA, the values were considered statistically significant when p < 0.05 and then a Bonferroni Post hoc test was applied. The suckling rats fed respectively with high doses of AlcH and AqH had a significantly higher body mass gain than the other groups (p < 0.05, one way ANOVA). Linear growth as measured by tibial length was significantly increased in the AqH group compared to the other groups. There was no significant difference in the mass and relative density of the tibia bones of the rats from the different treatment groups. The differences in growth could not be attributed to circulating concentrations of the somatotrophic hormone, Insulin-like growth factor-1 (IGF-1) which was not significantly different between the groups. The treatments did not result in any significant differences in lengths, and mass of the small and large intestine, however the caecum was significantly enlarged (hypertrophy of muscularis, submucosa and mucosa) in the rats that received the Aloe vera extracts. Although, there was no significant difference in the mass of the rats’ livers, the lipid and glycogen content were significantly higher (p < 0.001) for the AqH group compared to the other groups. Histologically, the hepatocytes showed enlarged nuclei, granular cytoplasm and dilated sinusoids for AqH and AlcH as compared to the control group. An indirect assessment of liver function by measurement of blood concentrations of alkaline phosphatase (ALP) and alanine amino transaminase (ALT) did not reveal a significant difference between the groups. The non fasting concentration of metabolic substrates (glucose and triglycerides) was also not significantly different between the groups. The pups given high doses of the extracts had a significantly greater (p < 0.05) thymus mass (hyperplastic) than the other groups. The short term administration of Aloe vera extracts has shown a growth promoting effect, enhanced hepatic storage of metabolic substrates and hypertrophy of the caecum and thymus of neonatal rats. These effects need to be explored further to enhance animal production and health.

碩士<br>國立中興大學<br>食品暨應用生物科技學系所<br>99<br>Hepatocellular carcinoma (HCC) is the high incidence and high mortality cancer which is the third cause of death from cancer in the world. HCC is also the first cause of cancer death in men and the second cause of cancer death in women in Taiwan; therefore, the novel treatment of HCC is an imperative need to develop. Cisplatin is one of the most widely used anti-cancer agents, but high dose administered to patient produce nephrotoxicity side effect and the dose of cisplatin must be limiting. Recently, studies have suggested that nature compounds or nutrients combined with low-dose cisplatin result a synergistic anti-cancer activity. Antrodia salmonea is a new species of the Antrodia and was first identified in 2004 which is only collected from the Cunninghamia konishii Hayata in Taiwan. Studies found that the extract of A. salmonea exhibited the anti-oxidative, anti-inflammation, anti-cancer ability and cytotoxic activity in vitro and in vivo. However, there is little information reported in the literature and the mechanisms underlying are still not clear. In this study, we investigated the apoptotic effect of the ethanol extract from Antrodia salmonea mycelia (AsE) in SK-Hep-1 cells, a highly invasive human hepatoma cell lines, and the effect of the combination of AsE and cisplatin and the possible mechanisms. 　　The results showed that the cell viability of SK-Hep-1 was concentration- and time-dependent suppression. At the 25, 75 and 125 μg/mL concentration of AsE combined with cisplatin in 48 h incubation had additive anti proliferation effect. We found that AsE induced apoptosis in SK-Hep-1. The main mechanism of the action included the cell cycle arrested at G0/ G1 phase, DNA damage and the activation of caspases. Furthermore, we combined AsE with cisplatin (40 μM) and found the synergistic increase in the rate of apoptosis. In 48 h incubation, a significant raise in sub-G1 phase of the combination of 125 μg/mL AsE and cisplatin. After treatment with cisplatin in combination with 75 and 125 μg/mL AsE for 48 h, significant DNA damage appeared and there was the synergistic damage to DNA. Otherwise, the 125 μg/mL concentration of AsE in combination with cisplatin increased caspases activity significantly, especially in 48 h, which synergistically promoted caspase-3 activity. 　　In conclution, we demonstrated that AsE inhibit the cell proliferation effectively in Sk-Hep-1 cells by inducing apoptosis. AsE in combination with cisplatin blocked the cell cycle progression at the G0/ G1 phase, elevated sub-G1 phase, induced DNA damage, activated caspases and culminated in the activation of apoptosis. AsE triggers apoptosis through intrinsic and extrinsic pathways, and the intrinsic pathway increase after combined with cisplatin. The combination of AsE and cisplatin exhibited a enhanced cytotoxic effect in SK-Hep-1 cells. These results warrant further studies on AsE as a potential adjuvant chemotherapeutic agents.