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Journal articles on the topic 'Alpha glycosidase'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

M., Amin Mir* Yangchan Dolma Tsering Yangchan Jigmat Stanzin Bilal Ahmad Mir. "ANTIDIABETIC PROPERTIES OF VARIOUS EXTRACTS OF HIPPOPHAE RHAMNOIDE." Indo American Journal of Pharmaceutical Sciences 04, no. 11 (2017): 4507–715. https://doi.org/10.5281/zenodo.1068273.

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The various extracts of Hippophae rhamnoide plant have been scrutinized for the anti-diabetic property by the use of alpha-amylase and alpha glycosidase enzymes inhibition method. The various parts of the plant have been extracted by Soxhlet extraction method, in which various solvents have been used in the order of their increasing polarity like Petroleum ether, Ethanol and Water. Acarbose have been used as a reference antidiabetic medicine by the inhibition of alpha-amylase and alpha glycosidase enzyme, the two key enzymes responsible for the degradation of higher carbohydrates into lower gl

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2

Sha, Bi Ying, Qing Shan Liu, Lin Cheng, and Xiao Ying Yin. "Preparation and Application of Core-Shell PMMA/Chitosan Nanoparticle." Advanced Materials Research 535-537 (June 2012): 271–74. http://dx.doi.org/10.4028/www.scientific.net/amr.535-537.271.

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Abstract: Objective To separate and enrich compounds from Apocynum venetum L. leaves. Methods The core-shell nanoparticles consisting of poly (methyl methacrylate) (PMMA) cores surrounded by various chitosan shells, induced by a tert-butylhydroperoxide (TBHP) solution. And core-shell nanoparticles was applied to immobilize Alpha glycosidase enzymes. Some compound from the extrat of Apocynum venetum L. leaves were absorbed by the immobilized Alpha glycosidase enzymes. and the components absorbed were detected by HPLC. Results Hyperoside and trifolin can be adsorbed by the nanoparticles immobili

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3

al Daher, S., G. Fleet, S. K. Namgoong, and B. Winchester. "Change in specificity of glycosidase inhibition by N-alkylation of amino sugars." Biochemical Journal 258, no. 2 (1989): 613–15. http://dx.doi.org/10.1042/bj2580613.

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The synthetic amino sugar 1,4-dideoxy-1,4-imino-L-allitol (DIA) is a moderately good inhibitor of human liver alpha-D-mannosidases and a weak inhibitor of alpha-L-fucosidase, N-acetyl-beta-D-hexosaminidase and beta-D-mannosidase. Methylation of the ring nitrogen of DIA markedly decreases the inhibition of all the glycosidases except N-acetyl-beta-D-hexosaminidase. N-Benzylation of DIA essentially abolishes all inhibitory activity, except towards alpha-L-fucosidase, which is more strongly inhibited than by either DIA or N-methyl-DIA. This is the first report of a change of specificity of inhibi

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4

Rura, Sarniati Rante, Yulia Yusrini Djabir, Abdul Rahim, Sri Ningsih, and Nisrina Firdausi. "Evaluation of the Anti-Diabetic Activity of Paliasa Leaf Ethanol Fraction (Kleinhovia hospita L) Through Inhibition of the Enzymes $\alpha$-Glucosidase and $\alpha$-Amylase." Jurnal Ilmiah Kesehatan (JIKA) 5, no. 2 (2023): 342–51. http://dx.doi.org/10.36590/jika.v5i2.509.

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Kleinhovia hospita has long been used to treat diseases, including diabetes mellitus. This study aimed to evaluate if the hypoglycemic effects of K. hospita extract and fractions were related to its inhibition on the activity of $\alpha$-amylase and $\alpha$-glycosidase enzymes. K. hospita leaves were extracted with 96% ethanol and then subjected to liquid-liquid partition using n-hexane, ethyl acetate, and water as solvents. The inhibitory capacity of ethanolic extract and its fractions were tested against $\alpha$-amylase and $\alpha$-glycosidase enzyme activity and compared to acarbose. The

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Widyawati, Paini Sri, Yesiana D. W. Werdani, and Christine Setiokusumo. "IN VITRO ANTIOXIDANT CAPACITIES AND ANTIDIABETIC PROPERTIES OF PLUCHEA LEAVES AND GREEN TEA MIXTURES AT VARIOUS PROPORTIONS." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 8 (2017): 203. http://dx.doi.org/10.22159/ijpps.2017v9i8.19545.

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Objective: This study was done to determine the effect of pluchea leaves and green tea mixtures at various proportions to antioxidant and antidiabetic properties.Methods: The research used a single factor randomized block design. Research factors were pluchea and green tea mixture proportions, including 100:0; 75:25; 50:50; 25:75; and 0:100% (w/w), respectively. Each of factors was repeated five times. The parameters observed in this study were phytochemical compounds, total phenols, total flavonoids, free radical DPPH scavenging activity, iron ion reducing power, alpha amylase and alpha glyco

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Field, R. A., A. H. Haines, E. J. T. Chrystal, and M. C. Luszniak. "Histidines, histamines and imidazoles as glycosidase inhibitors." Biochemical Journal 274, no. 3 (1991): 885–89. http://dx.doi.org/10.1042/bj2740885.

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This present study reports the ability of a range of derivatives of L-histidine, histamine and imidazole to act as inhibitors of sweet-almond β-glucosidase, yeast alpha-glucosidase and Escherichia coli β-galactosidase. The addition of a hydrophobic group to the basic imidazole nucleus greatly enhances binding to both the alpha- and β-glucosidases. L-Histidine (beta-naphthylamide (Ki 17 microM) is a potent competitive inhibitor of sweet-almond β-glucosidase as is omega-N-acetylhistamine (K1 35 microM), which inhibits the sweet-almond β-glucosidase at least 700 times more strongly than either ye

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7

Yan, Lin, Haiwei Xu, Fengwu Liu, Jin Zhao, and Hongmin Liu. "Synthesis and Biological Evaluation of Andrographolide C-Glycoside Derivatives as α-Glycosidase Inhibitors." Chinese Journal of Chemistry 30, no. 4 (2012): 914–18. http://dx.doi.org/10.1002/cjoc.201100179.

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8

Ortíz-Martinez, David Mizael, Catalina Rivas-Morales, Myriam Angelica de la Garza-Ramos, Maria Julia Verde-Star, Maria Adriana Nuñez-Gonzalez, and Catalina Leos-Rivas. "Miconiasp. Increases mRNA Levels of PPAR Gamma and Inhibits Alpha Amylase and Alpha Glucosidase." Evidence-Based Complementary and Alternative Medicine 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/5123519.

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Diabetes mellitus is a public health problem worldwide. For this reason, ethanolic extract ofMiconiasp. from Oaxaca, Mexico, was selected in search of an alternative against this disease. The effect ofMiconiasp. on mRNA expression of PPARγon cell line 3T3-L1, its effect on alpha amylase and alpha glucosidase, lipid accumulation during adipogenesis, and cell viability on VERO cells were evaluated. The mRNA levels of PPARγincreased on1.393±0.008folds, lipid accumulation was increased by 29.55% withMiconiasp. extract and 34.57% with rosiglitazone, andα-amylase andα-glycosidase were inhibited with

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9

Amin Mir, M., Shalini Upadhay, and Bilal Ahmad Mir. "Inhibition of Alpha Amylase and Alpha Glycosidase Enzymes by Various Earth Worm Extracts." Biomedical and Pharmacology Journal 11, no. 3 (2018): 1261–68. http://dx.doi.org/10.13005/bpj/1487.

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The earth worm extracts have been analysed for the inhibition of alpha amylase and alpha glycosidase enzymes, which are the key enzymes for the control of glucose in the human system so have direct impact upon the health of an organism. These two enzymes have direct involvement in the diabetic process. So the inhibition of these two enzymes helps in the reduction of diabetes. Various extracts of the earth worms, viz, Ethyl acetate, Acetone, DMSO, Ethanol and Water have been analysed against the inhibition of the respective enzymes. Among the various extracts it had been found that water extrac

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10

Kimura, Atsuo, Toshiyuki Nishio, Wataru Hakamada, et al. "Affinity Labelling of Glycosidase by .OMEGA.-Epoxyalkyl .ALPHA.-Glucoside." Journal of Applied Glycoscience 47, no. 2 (2000): 235–41. http://dx.doi.org/10.5458/jag.47.235.

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11

Shi, Hao, Yue Liu, Jiannan Guo та ін. "Thermostable manganese (II) dependent α-glycosidase from Pseudothermotoga thermarum". BioResources 14, № 3 (2019): 7266–74. http://dx.doi.org/10.15376/biores.14.3.7266-7274.

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Alpha-glycosidase degrades polysaccharides and oligosaccharides and participates in the synthesis of oligosaccharides through a process called transglycosylation. In this study, an α-glycosidase gene pthgly from Pseudothermotoga thermarum was cloned using pET-20b as a vector and was expressed in E. coli BL21(DE3). After heat treatment and affinity chromatography, the resulting recombinant enzyme was purified. The purity of the enzyme reached a single band at a molecular weight of approximately 55 kDa. The properties of the recombinant enzyme were determined. The optimal temperature of α-glycos

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12

Nash, Robert J., Barbara Bartholomew, Yana B. Penkova, and Ekaterina Kozuharova. "Iminosugars of the Invasive Arboreal Amorpha fruticosa and Glycosidase Inhibition Potential." Plants 14, no. 14 (2025): 2205. https://doi.org/10.3390/plants14142205.

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Amorpha fruticosa L. (Fabaceae) originates from North America and has become an aggressive invasive plant in many parts of the world. It affects the local biodiversity in many negative ways. Our previous in vivo tests of purified extract of A. fruticosa pods for antihyperglycemic activity in streptozotocin-induced diabetic spontaneously hypertensive rats (SHRs) revealed that the oral administration of purified extract of A. fruticosa (100 mg/kg) for 35 days to SHRs caused significant decreases in the systolic pressure, blood glucose levels, and MDA quantity. The aim of this experimental study

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13

Sperti, Simonetta, Mariacristina Zamboni, Maurizio Brigotti, Fioretta Rambelli, and Lucio Montanaro. "Alpha-sarcin impairs the N-glycosidase activity of ricin on ribosomes." Biochemical and Biophysical Research Communications 160, no. 2 (1989): 857–61. http://dx.doi.org/10.1016/0006-291x(89)92513-8.

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14

Benešová, E., M. Marková, and B. Králová. " α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2." Czech Journal of Food Sciences 23, No. 3 (2011): 116–20. http://dx.doi.org/10.17221/3380-cjfs.

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In this work six psychophilic and psychrotrophic bacterial strains were screened for the presence of different glycosidase activities (&alpha;-galactosidase, &alpha;-glucosidase, &beta;-glucosidase, &alpha;-mannosidase and &beta;-glucuronidase). Nine enzymes were found and their elementary characteristics were measured (t<sub>optimum</sub>, pH<sub>optimum</sub>, K<sub>m</sub>, V<sub>lim</sub>).Two enzymes with the highest activities at low temperatures were chosen for the next study, i.e. &alpha;-glucosidase and &beta;

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15

Sun, Y., and W. J. Ball. "Determination of Na(+)-K(+)-ATPase alpha- and beta-isoforms and kinetic properties in mammalian liver." American Journal of Physiology-Cell Physiology 262, no. 6 (1992): C1491—C1499. http://dx.doi.org/10.1152/ajpcell.1992.262.6.c1491.

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While Western blot analysis clearly revealed the presence of the alpha- and beta-subunits of Na(+)-K(+)-ATPase in a variety of rat tissues, beta was not readily detectable in liver. This observation was consistent with a previous report indicating that Na(+)-K(+)-ATPase immunoprecipitated from rat liver gives no clear evidence for the presence of a beta-subunit (Hubert et al. Biochemistry 25: 4156-4163, 1986). However, Western blot analysis of density gradient-purified lamb and rat liver microsomes showed the presence of a protein with an approximate molecular mass of 42 kDa that was immunorea

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16

Kęska, Paulina, Joanna Stadnik, Aleksandra Łupawka та Agata Michalska. "Novel α-Glucosidase Inhibitory Peptides Identified In Silico from Dry-Cured Pork Loins with Probiotics through Peptidomic and Molecular Docking Analysis". Nutrients 15, № 16 (2023): 3539. http://dx.doi.org/10.3390/nu15163539.

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Diabetes mellitus is a serious metabolic disorder characterized by abnormal blood glucose levels in the body. The development of therapeutic strategies for restoring and maintaining blood glucose homeostasis is still in progress. Synthetic alpha-amylase and alpha-glucosidase inhibitors can improve blood glucose control in diabetic patients by effectively reducing the risk of postprandial hyperglycemia. Peptides of natural origin are promising compounds that can serve as alpha-glucosidase inhibitors in the treatment of type 2 diabetes. Potential alpha-glucosidase-inhibiting peptides obtained fr

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17

Nishio, Toshiyuki. "Effect of Sugar Hydroxyl Groups on Activities and Specificities of .ALPHA.-Glycosidase and Lipase." Journal of Applied Glycoscience 49, no. 1 (2002): 45–55. http://dx.doi.org/10.5458/jag.49.45.

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18

Nishio, Toshiyuki, Wataru Hakamata, Masahiro Ogawa, et al. "Investigations of a Useful .ALPHA.-Glycosidase for the Enzymatic Synthesis of Rare Sugar Oligosaccharides." Journal of Applied Glycoscience 52, no. 2 (2005): 153–60. http://dx.doi.org/10.5458/jag.52.153.

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19

Saniya, A., R. Divya, M. Sharmila, and C. Prakash. "ANTI-DIABETIC AND ANTIMICROBIAL ACTIVITIES OF GRONA TRIFLORA MEDICINAL PLANT." Archives for Technical Sciences 32, no. 1 (2025): 176–87. https://doi.org/10.70102/afts.2025.1732.176.

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This research analyses the bioactive Components of Grona Triflora, a medicinal plant that has potential health benefits, and its application in textile finishing to enhance fabric functionality and property. Grona Triflora was purified, dried, and ground into a powdered material for extraction using methanol, ethanol, and distilled water. The extraction’s, anti-diabetic activity of each extract was analyzed using the alpha-amylase and alpha-glycosidase biocatalyst prevention assays to determine effectiveness in modulating polysaccharide metabolism. The Anti-microbial activity was assessed agai

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20

Bolmer, Sally D., and Jerome Kleinerman. "Altered Glycosidase Activity in the Liver of Rats with Galactosamine-Induced Alpha(1)-Antiprotease Deficiency." Enzyme 34, no. 3 (1985): 144–51. http://dx.doi.org/10.1159/000469377.

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21

Gopal, B. Anitha, Sridevi A. Singh, and G. Muralikrishna. "Porcine pancreatic alpha amylase and its isoforms—Effect of deglycosylation by peptide-N-glycosidase F." International Journal of Biological Macromolecules 43, no. 2 (2008): 100–105. http://dx.doi.org/10.1016/j.ijbiomac.2008.03.008.

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22

Karaulova, E. P., H. D. Yoon, J. G. Kim, S. H. Park, T. N. Slutskaya, and E. V. Yakush. "Study on biological activity of tissues from bivalve mollusks." Izvestiya TINRO 195 (December 27, 2018): 253–64. http://dx.doi.org/10.26428/1606-9919-2018-195-253-264.

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Biological activity of tissues and enzyme hydrolyzates is investigated for three species of bivalve mollusks:Corbicula japonica,Mercenaria mercenaria, andAnadara broughtonii. Antiradical activity of all samples has increased signifcantly during their enzymatic hydrolysis: in eight times forC. japonica, in three times forM. mercenaria, and in 2.5 times forA. broughtonii. The highest anti-trombolitic activity (0.0823 units) is found for the hydrolyzate ofC. japonicain concentration of 10 mg/mL. Angiotensin I-converting enzyme inhibitory activity is found only for the hydrolyzate ofM. mercenariai

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23

Frengki, Frengki, Deddi Prima, Fatma Sri Wahyuni, et al. "UJI IN VITRO DAN IN SILICO SENYAWA 5,7,2’,5’-TETRAHYDROXY FLAVAN-3-OL TERHADAP ENZIM ALPHA GLUCOSIDASE." Jurnal Fitofarmaka Indonesia 5, no. 2 (2018): 279–83. http://dx.doi.org/10.33096/jffi.v5i2.416.

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Several of Calophyllums genus have been searched and proven as medicinal plants and Calophyllum macrophyllum is one of its genus. We have been isolated a compound from ethyl acetate fraction of the stem-bark. The compound was flavan-3-ol (5,7,2’,5’-tetrahydroxy flavan-3-ol). This research aimed to determine inhibition antidiabetic activity and affinity of its compound on α-glycosidase enzyme. In vitro antidiabetic effect shown by IC50 9.10 µg/ml and docking by Arguslab 4.01 shown by ΔG -10,24 kkal/mol as in silico test. The inhibition activity of flavan-3-ol against the α-glucosidase enzyme th

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Donald, A. S. R., and J. Feeney. "Oligosaccharides obtained from a blood-group-Sd(a+) Tamm-Horsfall glycoprotein. An n.m.r. study." Biochemical Journal 236, no. 3 (1986): 821–28. http://dx.doi.org/10.1042/bj2360821.

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Treatment of Tamm-Horsfall urinary glycoprotein with Bacteroides fragilis endo-beta-galactosidase over a range of enzyme concentrations, pH and temperature resulted in the release of a small but constant proportion of the terminal sugars, which indicates the presence in the glycoprotein of relatively few enzyme-susceptible -GlcNAc beta 1-3Gal beta 1-4GlcNAc- units. Three oligosaccharides were isolated from the enzyme digest and characterized as Gal beta 1-4GlcNAc beta 1-3Gal, NeuAc alpha 2-3Gal beta 1-4 GlcNAc beta 1-3Gal and GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-4GlcNAc beta 1-3Gal by me

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Keinänen, K. P. "Effect of deglycosylation on the structure and hormone-binding activity of the lutropin receptor." Biochemical Journal 256, no. 3 (1988): 719–24. http://dx.doi.org/10.1042/bj2560719.

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Affinity-purified rat ovarian lutropin (LH) receptor is a single 90 kDa polypeptide which binds to immobilized lectins, indicating that the receptor is a glycoprotein [Keinänen, Kellokumpu, Metsikkö & Rajaniemi (1987) J. Biol. Chem. 262, 7920-7926]. In the present study the glycoprotein nature of the rat ovarian LH receptor was investigated in order to determine the contribution of the glycan moiety to receptor's size and hormone-binding properties. Treatment of the 125I-labelled purified LH receptor with neuraminidase and peptide N-glycosidase F resulted in a decrease in size of LH recept

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Kaur, Virender, Kumud Upadhyaya, and Milind Pande. "BIOASSAY-GUIDED EVALUATION OF FICUS SEMICORDATA FOR ANTIDIABETIC ACTIVITY." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 3 (2017): 71. http://dx.doi.org/10.22159/ijpps.2017v9i3.16441.

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Objective: The early stage of diabetes mellitus type 2 is associated with postprandial hyperglycemia. The therapeutic approach involved in the treatment of type 2 diabetes mellitus is the use of agents that can decrease postprandial hyperglycemia by inhibiting carbohydrate digesting enzymes. In an effort of identifying herbal drugs which may become useful in the prevention or mitigation of diabetes, the antidiabetic activity of Ficus semicordata (FS) and its constituents were studied. The present study was undertaken in part to identify the potent antihyperglycemic fraction from the ethanol ex

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Hosie, L., та M. L. Sinnott. "Effects of deuterium substitution α and β to the reaction centre, 18O substitution in the leaving group, and aglycone acidity on hydrolyses of aryl glucosides and glucosyl pyridinium ions by yeast α-glucosidase. A probable failure of the antiperiplanar-lone-pair hypothesis in glycosidase catalysis". Biochemical Journal 226, № 2 (1985): 437–46. http://dx.doi.org/10.1042/bj2260437.

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Neither kcat. nor kcat./Km for five aryl alpha-D-glucopyranosides correlates with aglycone pKa, and isotope effects, described according to the convention used by Cleland [(1982) CRC Crit. Rev. Biochem. 13, 385-428], of 18(V) = 1.002 +/- 0.008, alpha D(V) = 1.01 +/- 0.04 and alpha D(V/K) = 0.969 +/- 0.035 are observed for p-nitrophenyl, and one of beta D(V) = 1.02 +/- 0.04 for phenyl alpha-D-glucopyranoside; kcat. but not kcat./Km, correlates with aglycone pKa for five alpha-D-glucopyranosyl pyridinium ions with a Brønsted coefficient of -0.61 +/- 0.06, and isotope effects of alpha D(V) = 1.22

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Terman, B. I., J. F. Reece, R. D. Brown та P. A. Insel. "The oligosaccharide component of α 1-adrenergic receptors from BC3H1 and DDT1 muscle cells. Studies with glycosidases and photoaffinity labelling of intact cells". Biochemical Journal 253, № 2 (1988): 363–70. http://dx.doi.org/10.1042/bj2530363.

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In this study, we clarify the structural aspects of the oligosaccharides associated with the alpha 1-adrenergic receptor in two muscle cell lines. Photoaffinity labelling of intact BC3H1 or DDT1 muscle cells with 2-[4-(4-azido-3-[125I]iodobenzoyl)piperazin-1-yl]-4-amino-6, 7-dimethoxyquinazoline ([125I]azidoprazosin) followed by SDS/polyacrylamide-gel electrophoresis (PAGE) and autoradiography revealed specifically labelled proteins of molecular mass = 87,000 and 81,000, respectively. Treatment of photoaffinity-labelled receptors in DDT1 cells with 33 u. of endoglycosidase F/ml for 24 h result

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Dohi, T., A. Nishikawa, I. Ishizuka, et al. "Substrate specificity and distribution of UDP-GalNAc:sialylparagloboside N-acetylgalactosaminyltransferase in the human stomach." Biochemical Journal 288, no. 1 (1992): 161–65. http://dx.doi.org/10.1042/bj2880161.

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The detailed substrate specificity of the UDP-GalNAc:sialylparagloboside N-acetylgalactosaminyltransferase to form the Sd(a+) blood group active carbohydrate determinant GalNAc beta 1-4(NeuAc alpha 2-3)Gal was studied using a membrane fraction prepared from human gastric fundic mucosa. Various sialosylated oligosaccharides and gangliosides were examined as acceptor substrates. Oligosaccharide substrates were fluorescence-labelled with 2-aminopyridine, and the transferase activity was quantified by h.p.l.c. using a reversed-phase column. The structures of the products were determined by glycosi

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Decastel, M., M. A. Doyennette-Moyne, E. Gouet, M. Aubery, and P. Codogno. "Biosynthesis, surface expression and function of the fibronectin receptor after rat liver cell transformation to tumorigenicity." Biochemical Journal 291, no. 1 (1993): 247–55. http://dx.doi.org/10.1042/bj2910247.

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Zajdela hepatoma cells are poorly-adherent cells derived from an undifferentiated tumour and transplanted into rat. We compared the biosynthesis, structure and function of the fibronectin receptor in normal rat hepatocytes with that in Zajdela hepatoma cells. The rat hepatocyte fibronectin receptor has been isolated. It is composed of two subunits: alpha 5 (molecular mass 155 kDa) and beta 1 (molecular mass 115 kDa). However, its biosynthesis has not yet been described. Using polyclonal antibodies raised against each of the subunits of the receptor, we observed that the alpha 5-subunit was syn

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McGuire, E. J., R. Kerlin, J. J. Cebra, and S. Roth. "A human milk galactosyltransferase is specific for secreted, but not plasma, IgA." Journal of Immunology 143, no. 9 (1989): 2933–38. http://dx.doi.org/10.4049/jimmunol.143.9.2933.

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Abstract IgA from human milk and colostrum is a substrate for a galactosyltransferase also present in milk and colostrum. The secreted IgA that serves as the best acceptor for the transferase activity is the IgA that fails to bind readily to jacalin lectin. Upon becoming galactosylated by the transferase, however, the IgA shows an increased affinity for jacalin. Glycosidase and electrophoretic results indicate that the transferred galactose is beta-linked to the alpha-chain of the IgA. The IgA:transferase activity can be purified by gel filtration and cation exchange chromatographies, as well

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Mach, L., W. Scherf, M. Ammann, et al. "Purification and partial characterization of a novel lectin from elder (Sambucus nigra L.) fruit." Biochemical Journal 278, no. 3 (1991): 667–71. http://dx.doi.org/10.1042/bj2780667.

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A previously unknown haemagglutinin, named Sambucus nigra agglutinin-III (SNA-III), has been purified from the fruit of the elder (Sambucus nigra). Whereas elder bark agglutinin I (SNA-I) is highly specific for terminal alpha 2,6-linked sialic acid residues, SNA-III displays a high affinity for oligosaccharides containing exposed N-acetylgalactosamine and galactose residues. Different N-terminal sequences and the amino acid composition distinguish the fruit lectin from elder bark agglutinin II (SNA-II), which shows a similar carbohydrate specificity. The 40-fold higher affinity of SNA-III for

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Sato, N., C. Caux, T. Kitamura, et al. "Expression and factor-dependent modulation of the interleukin-3 receptor subunits on human hematopoietic cells." Blood 82, no. 3 (1993): 752–61. http://dx.doi.org/10.1182/blood.v82.3.752.752.

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Abstract Interleukin-3 (IL-3) regulates growth and differentiation of multipotential as well as lineage-committed progenitor cells. The human IL-3 receptor (IL-3R) consists of the alpha and common beta (beta c) subunits. The alpha subunit (IL-3R alpha) is specific for IL-3 and binds IL-3 with low affinity. In contrast, the beta c subunit does not bind any cytokine by itself, but forms a high-affinity receptor with IL- 3R alpha. As the same beta c subunit also forms high-affinity receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) with the respective cytokine-specif

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Sato, N., C. Caux, T. Kitamura, et al. "Expression and factor-dependent modulation of the interleukin-3 receptor subunits on human hematopoietic cells." Blood 82, no. 3 (1993): 752–61. http://dx.doi.org/10.1182/blood.v82.3.752.bloodjournal823752.

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Interleukin-3 (IL-3) regulates growth and differentiation of multipotential as well as lineage-committed progenitor cells. The human IL-3 receptor (IL-3R) consists of the alpha and common beta (beta c) subunits. The alpha subunit (IL-3R alpha) is specific for IL-3 and binds IL-3 with low affinity. In contrast, the beta c subunit does not bind any cytokine by itself, but forms a high-affinity receptor with IL- 3R alpha. As the same beta c subunit also forms high-affinity receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) with the respective cytokine-specific alpha

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Dahms, N. M., and G. W. Hart. "Lymphocyte function-associated antigen 1 (LFA-1) contains sulfated N-linked oligosaccharides." Journal of Immunology 134, no. 6 (1985): 3978–86. http://dx.doi.org/10.4049/jimmunol.134.6.3978.

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Abstract The murine lymphocyte function-associated antigen 1 (LFA-1) is a glycoprotein heterodimer consisting of an Mr 180,000 alpha-chain and an Mr 95,000 beta-chain. Although LFA-1 has been studied extensively in the past few years due to its involvement in various antigen-specific T lymphocyte responses, virtually nothing is known about its glycosylation. In this report, we have analyzed the oligosaccharide moieties of the murine LFA-1 molecule. Utilizing a T lymphoma cell line, EL-4, it was found that [35S] sulfate, [3H]glucosamine, [3H]mannose, and [3H]fucose were incorporated into both t

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Kiyohara, T., J. W. Dennis, R. J. Boegman, and J. C. Roder. "An exoglycosidase-sensitive triggering site on NK cells which is coupled to transmethylation of membrane phospholipids." Journal of Immunology 135, no. 1 (1985): 659–64. http://dx.doi.org/10.4049/jimmunol.135.1.659.

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Abstract Glycosidic enzymes were used as probes to analyze the mechanism of NK cell-mediated cytotoxicity. Pretreatment of nylon wool-enriched CBA/J spleen cells, a murine NK clone, or human peripheral blood lymphocytes (PBL) with alpha-mannosidase, an exoglycosidase, led to a marked dose-dependent inhibition of NK lytic activity against YAC-1.2 or K562 tumor cells. Maximal inhibition occurred after a 60-min pretreatment of murine effectors at 37 degrees C, and the kinetics of NK inhibition by alpha-mannosidase was similar to the reported kinetics for enzymatic activity. Released hexose was de

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Dogara, Abdulrahaman Mahmoud, Sawsan Sadiq Al-Rawi, Ateeq Ahmed Al-Zahrani, et al. "Biological Activities, Chemical Composition and Molecular Docking of Urelytrum giganteum Pilg." Acta Chimica Slovenica 72, no. 1 (2025): 107–18. https://doi.org/10.17344/acsi.2024.9077.

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The growing interest in health-promoting food options has highlighted the need to explore new sources with unique nutritional compositions. Plants are rich sources of bioactive compounds with biological effects. Urelytrum giganteum Pilg is a perennial wild tufted grass that grows in dry tropical African countries. Traditionally, it has been used to treat several diseases, however, up to date, no study has been done on this plant. This study analyzed the solvent extraction of Urelytrum giganteum Pilg, a perennial wild tufted grass in dry tropical African countries, to determine its proximate an

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Petäjä-Repo, U. E., W. E. Merz, and H. J. Rajaniemi. "Significance of the carbohydrate moiety of the rat ovarian luteinizing-hormone/chorionic-gonadotropin receptor for ligand-binding specificity and signal transduction." Biochemical Journal 292, no. 3 (1993): 839–44. http://dx.doi.org/10.1042/bj2920839.

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The contribution of the carbohydrate moiety of the rat ovarian luteinizing-hormone (LH)/chorionic-gonadotropin (CG) receptor to ligand-binding specificity and signal transduction was investigated by using glycosidases. Purified membranes from pseudo-pregnant rat ovaries were treated with neuraminidase or peptide N-glycosidase F, to remove terminal sialic acids and N-linked oligosaccharides of the receptor, respectively. Ligand blotting and densitometric scanning of the autoradiograms showed that 90-95% of the receptors were deglycosylated, and that desialylation was virtually complete. Neither

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Holt, G. D., S. J. Swiedler, J. H. Freed, and G. W. Hart. "Murine Ia-associated invariant chain's processing to complex oligosaccharide forms and its dissociation from the I-Ak complex." Journal of Immunology 135, no. 1 (1985): 399–407. http://dx.doi.org/10.4049/jimmunol.135.1.399.

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Abstract The processing of murine invariant chain (Ii) to a cell surface form bearing complex N-linked oligosaccharides has been demonstrated in the B cell lymphoma, AKTB-1b. In addition, the rate of processing of pulse-labeled Ii has been determined relative to its rate of dissociation from the alpha/beta complex of I-Ak. Ii, alpha-, and beta-chains were immunoprecipitated with anti-I-Ak or anti-Ii monoclonal antibodies. The heretofore uncharacterized complex oligosaccharide form of Ii (Ii-c) was identified in gel-purified immunoprecipitates by peptide mapping with reverse-phase HPLC. Ii-c is

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Ito, N., K. Nishi, M. Nakajima, Y. Okamura, and T. Hirota. "Histochemical analysis of the chemical structure of blood group-related carbohydrate chains in serous cells of human submandibular glands using lectin staining and glycosidase digestion." Journal of Histochemistry & Cytochemistry 37, no. 7 (1989): 1115–24. http://dx.doi.org/10.1177/37.7.2499620.

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Using lectin staining methods in combination with exo- and endo-glycosidase digestion procedures, we analyzed the chemical structure of different types of blood group-related substances in serous cells of formalin-fixed, paraffin-embedded human submandibular glands. Serous cells produced only H antigen; A and B antigens were not present, and the expression of H antigen is dependent on the secretor status of the tissue donor. Although reactivity with Ulex europaeus agglutinin I (UEA-I) was not markedly reduced by alpha-L-fucosidase digestion, an affinity for peanut agglutinin (PNA) was seen aft

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Najjar, S. M., L. T. Hampp, R. Rabkin, and G. M. Gray. "Sucrase-alpha-dextrinase in diabetic BioBreed rats: reversible alteration of subunit structure." American Journal of Physiology-Gastrointestinal and Liver Physiology 260, no. 2 (1991): G275—G283. http://dx.doi.org/10.1152/ajpgi.1991.260.2.g275.

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BioBreed (BB) Wistar rats develop diabetes mellitus, which closely resembles the human disease, in 50% of progeny. Intestinal sucrase-alpha-dextrinase, a glycoprotein hydrolase of the enterocyte's brush border consisting of 140-kDa alpha-dextrinase and 125-kDa sucrase subunits, is essential for surface digestion of carbohydrate nutrients. Although its catalytic characteristics were found to be maintained in the diabetic state, the structure of the subunits, as compared with normal Wistar rats, was altered in the BB rat within 2 days of the onset of diabetes. Its capacity to react in a solid-ph

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Lityńska, Anna, Ewa Pocheć, Dorota Hoja-Lukowicz, et al. "The structure of the oligosaccharides of alpha3beta1 integrin from human ureter epithelium (HCV29) cell line." Acta Biochimica Polonica 49, no. 2 (2002): 491–500. http://dx.doi.org/10.18388/abp.2002_3808.

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There is a growing line of evidence that glycosylation of alpha and beta subunits is important for the function of integrins. Integrin alpha3beta1, from human ureter epithelium cell-line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sulfate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide N-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spectrome

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Axamawaty, M. T. H., G. W. J. Fleet, K. A. Hannah, S. K. Namgoong та M. L. Sinnott. "Inhibition of the α-l-arabinofuranosidase III of Monilinia fructigena by 1,4-dideoxy-1,4-imino-l-threitol and 1,4-dideoxy-1,4-imino-l-arabinitol". Biochemical Journal 266, № 1 (1990): 245–49. http://dx.doi.org/10.1042/bj2660245.

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1. 1,4-Dideoxy-1,4-imino-L-threitol was synthesized and the synthesis of 1,4-dideoxy-1,4-imino-L-arabinitol was improved. 2. Both compounds are competitive inhibitors of Monilinia fructigena alpha-L-arabinofuranosidase III, the additional hydroxymethyl group in the arabinitol contributing about 17.8 kj/mol (4.25 kcal/mol) to the Gibbs free energy of binding. 3. The affinities (1/Ki) of both compounds vary with pH in a classical bell-shaped way, the pKa value being that of the acid-catalytic group on the enzyme [5.9; Selwood & Sinnott (1988) Biochem. J. 254, 899-901] and the pKb values bein

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P. Samba Siva Rao, P. Samba Siva Rao, Dr G. Nagaraju Dr. G. Nagaraju, A. Sanjay A. Sanjay, et al. "Review on Formulation and Evaluation of Voglibose Mouth Dissolving Tablets." International Journal of Pharmaceutical Research and Applications 10, no. 2 (2025): 309–17. https://doi.org/10.35629/4494-1002309317.

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Mouth dissolving tablets are solid dosage forms which break down in the oral cavity less than one minute without using of water. These dosage forms are placed in the mouth, allowable to diffuse or melt in the saliva. The purpose of this article is to review potential advancements of Oral Dispersible Tablet technology in drug delivery applications. Mouth dissolving Voglibose tablets offer a convenient and patient-friendly alternative to traditional tablets for the management of type 2 diabetes mellitus. With their rapid disintegration and absorption, these tablets provide a quick and effective

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Maecker, H. T., and R. Levy. "Spontaneous T cell antigen receptor variants of a human T leukemia cell line." Journal of Immunology 141, no. 9 (1988): 2994–3002. http://dx.doi.org/10.4049/jimmunol.141.9.2994.

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Abstract We have sought to address the question of clonal variation of TCR within a human T leukemia cell line, HPB-ALL. To do so, a panel of anti-idiotypic antibodies was produced and the cell line examined for variants. We isolated both spontaneous idiotype and receptor-negative variants without applying mutagens or any selective pressure other than sorting the cells. These sorted and cloned populations are all clonally related to each other as shown by their beta-TCR locus gene rearrangements. The idiotype variants have alpha-chains which are differentially glycosylated, but they have the s

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Priyanka. "Anti-Diabetic Activity of Selected Medicinal Plant Extracts Used By Tribals in the Adilabad District of Telangana State By in Vitro." Biolife 11, no. 2 (2023): 72–78. https://doi.org/10.5281/zenodo.7855153.

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<strong>Abstract</strong> Diabetes mellitus is a metabolic condition marked by high blood glucose levels as well as changes in carbohydrate, lipid, and protein metabolism. The goal of this study was to determine the inhibitory effect of selected plants (<em>Artemisia vulgaris</em> - root, and <em>Angelica archangelica</em> - leaves) on -glucosidase and -amylase to assess anti-diabetic effectiveness in vitro. By using the Soxhlet extraction method, each plant powder was repeatedly extracted with different organic solvents of increasing polarity. To investigate invitro anti-diabetic efficacy, th

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Thibaudeau, K., L. Borche, JP Soulillou, and D. Blanchard. "Characterization of porcine platelet glycoproteins recognized by human natural "anti-gal" antibodies." Blood 87, no. 11 (1996): 4636–42. http://dx.doi.org/10.1182/blood.v87.11.4636.bloodjournal87114636.

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Human natural “anti-Gal” antibodies are specifically directed to Gal alpha 1–3Gal beta 1–4GlcNAc residues expressed on non-primate mammal and new world monkey cells. We investigated the relative involvement of purified IgG and IgM anti-Gal as xenoreactive natural antibodies (XNA). IgG and IgM were isolated from human plasma, and anti-Gal antibodies were purified by affinity chromatography on a Synsorb-14 column (Chembiomed, Edmonton, Alberta, Canada). Anti-Gal of both IgM and IgG classes represent the bulk of human XNA that bind to porcine platelets in enzyme-linked immunosorbent assay (ELISA)

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Hidaka, H., and N. H. Fidge. "Affinity purification of the hepatic high-density lipoprotein receptor identifies two acidic glycoproteins and enables further characterization of their binding properties." Biochemical Journal 284, no. 1 (1992): 161–67. http://dx.doi.org/10.1042/bj2840161.

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Several high-density lipoprotein (HDL)-binding proteins, candidates for the putative HDL receptor, have recently been identified, including two membrane proteins: HB1 of 120 kDa and HB2 of 100 kDa, present in rat and human liver plasma membranes respectively. Further insights into their function however, have been hampered by poor recoveries of these hydrophobic peptides, and the present work was undertaken to improve yields and enable a more detailed investigation of their properties. A significant improvement has been achieved using two affinity chromatographic procedures, one exploiting the

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De Oliveira, Letícia Gomes, Júlia Carneiro Almeida, Carlos Alberto Mourão Júnior, and Ana Eliza Andreazzi. "Efeitos dos inibidores de alfa-amilase e alfa-glicosidase no tratamento da obesidade: uma revisão integrativa / Effects of alpha-amylase and alpha-glycosidase inhibitors on obesity treatment: an integrative review." Brazilian Journal of Health Review 4, no. 6 (2021): 26125–41. http://dx.doi.org/10.34119/bjhrv4n6-200.

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Berry, Leslie R., and Anthony K. C. Chan. "Age-Related Differences in the Glycosylation of Anticoagulant Protein C." Blood 112, no. 11 (2008): 2031. http://dx.doi.org/10.1182/blood.v112.11.2031.2031.

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Abstract Thromboembolic events can result from procoagulant stimuli and the inability of native anticoagulant mechanisms to pacify thrombin generation. In the pediatric population, although thrombosis occurs in the presence of major coagulant insults, children are relatively resistant to onset of thrombotic complications. A key aspect contributing to developmental differences in the young is that coagulation factors and inhibitors have been shown to exist in different plasma concentrations and developmental forms compared to adults. Increasing evidence indicates that protein C (PC) is a major

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