Relevant bibliographies by topics / Alphat

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Academic literature on the topic 'Alphat'

Author: Grafiati
Published: 26 July 2024

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Journal articles on the topic "Alphat"

1

DiSanto, J. P., D. Guy-Grand, A. Fisher, and A. Tarakhovsky. "Critical role for the common cytokine receptor gamma chain in intrathymic and peripheral T cell selection." Journal of Experimental Medicine 183, no. 3 (March 1, 1996): 1111–18. http://dx.doi.org/10.1084/jem.183.3.1111.

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The common cytokine receptor gamma chain (gammac), which is a functional subunit of the receptors for interleukins (IL)-2, -4, -7, -9, and -15, plays an important role in lymphoid development. Inactivation of this molecule in mice leads to abnormal T cell lymphopoiesis characterized by thymic hypoplasia and reduced numbers of peripheral T cells. To determine whether T cell development in the absence of gammac is associated with alterations of intrathymic and peripheral T cell selection, we have analyzed gammac-deficient mice made transgenic for the male-specific T cell receptor (TCR) HY (HY/gammac- mice). In HY/gammac- male mice, negative selection of autoreactive thymocytes was not diminished; however, peripheral T cells expressing transgenic TCR-alpha and -beta chains (TCR-alphaT/betaT) were absent, and extrathymic T cell development was completely abrogated. In HY/gammac- female mice, the expression of the transgenic TCR partially reversed the profound thymic hypoplasia observed in nontransgenic gammac- mice, generating increased numbers of thymocytes in all subsets, particularly the TCR-alphaT/betaT CD8+ single-positive thymocytes. Despite efficient positive selection, however, naive CD8+ TCR-alphaT/betaT T cells were severely reduced in the peripheral lymphoid organs of HY/gammac- female mice. These results not only underscore the indispensible role of gammac in thymocyte development, but also demonstrate the critical role of gammac in the maintenance and/or expansion of peripheral T cell pools.
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Skoglund, G., A. Basmaciogullari, B. Rouot, JC Marie, and G. Rosselin. "Cell-specific localization of G protein alpha-subunits in the islets of Langerhans." Journal of Endocrinology 162, no. 1 (July 1, 1999): 31–37. http://dx.doi.org/10.1677/joe.0.1620031.

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G protein alpha-subunits are involved in the transduction of receptor-mediated regulation of insulin and glucagon secretions. To get further insight into the status of G proteins in alpha- and beta-cells of the Langerhans islets, we have used immunohistochemistry to study the distribution of alpha-subunits in pancreas sections from the rat. Our results show that only insulin-immunoreactive beta-cells display immunoreactivity for selective antibodies directed against the different members of the Galphas and Galpha12-families (alphas, alphaolf, and alpha12, alpha13 respectively). Immunoreactivities for antibodies directed against members of the Galphaq- and Galphai-families showed a more diverse localization: alpha11 and alphao2 were only detected in glucagon-immunoreactive alpha-cells, whereas alphai1 was detected in all beta-cells but only in a few alpha-cells. Even though beta-cells showed immunoreactivities for alphao-non-isoform-selective antibodies, we could not identify the isoform(s) present using selective alphao1 and alphao2 antibodies. Other members of the Galphai- and Galphaq-families (alphai3, alphat2, alphaz and alphaq) were detected in both alpha- and beta-cells. In conclusion, our findings demonstrate a clear difference in the localization of G protein alpha-subunits between alpha- and beta-cells, suggesting the involvement of specific receptor transduction pathways for the neuronal/hormonal regulation of alpha- and beta-cell functions.
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Pasenkiewicz-Gierula, M., and T. Róg. "Conformations, orientations and time scales characterising dimyristoylphosphatidylcholine bilayer membrane. Molecular dynamics simulation studies." Acta Biochimica Polonica 44, no. 3 (September 30, 1997): 607–24. http://dx.doi.org/10.18388/abp.1997_4409.

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The results of molecular dynamics simulation of fully hydrated dimyristoylphosphatidylcholine (DMPC) bilayer membrane in the liquid-crystalline phase are presented. They show that the probability of a gauche conformation varies periodically along the chain with only a slight increase towards the end of the chain. However, the frequency of transition between conformations increases, due to a decrease in the lifetime of the trans conformation, along the chain. The average lifetimes for trans conformations are in the range of 1-2 x 10(-10) s and for gauche conformations in the range of 4-7 x 10(-11) s. The alpha-chain of the DMPC head group has mainly an extended conformation, due to predominantly trans conformation of alpha5 torsion. The rotational correlation time for the P-N vector is 3.7 ns. The C2-C1-O11-P fragment of the DMPC head group (theta1, alpha1, alpha2 torsions) is rigid while the P-O12-C11-C12 fragment (alpha3, alpha4, alpha5 torsions) is flexible. The lateral diffusion coefficient for DMPC self-diffusion in the membrane is 2 x 10(-7) cm2/s; the rate of transverse diffusion is the same. Large differences in the calculated rotational correlation times for the alpha-, beta-, gamma-chains and for the O21-C1 vector indicate that in the liquid-crystalline bilayer each segment of the DMPC molecule exhibits its own rotational freedom, in addition to its internal flexibility resulting from rotational isomerism. The results obtained in these calculations, although in general agreement with some experimental data, shed new light on the dynamical behaviour of phosphatidylcholine molecules in the bilayer membrane in the liquid-crystalline phase.
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Jacques, T. S., J. B. Relvas, S. Nishimura, R. Pytela, G. M. Edwards, C. H. Streuli, and C. ffrench-Constant. "Neural precursor cell chain migration and division are regulated through different beta1 integrins." Development 125, no. 16 (August 15, 1998): 3167–77. http://dx.doi.org/10.1242/dev.125.16.3167.

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Proliferation and tangential migration of neural precursor cells are essential determinants of CNS development. We have established cell culture models of both these processes using neural precursor cells grown as neurospheres. The pattern of migration that we observe in these cells is homotypic and occurs in the absence of a glial or neuronal scaffold, and is therefore equivalent to that previously described as chain migration. To determine the role of integrins in proliferation and migration, we have analysed the expression pattern of integrins on neurosphere cells and then performed blocking peptide and antibody experiments. Neurosphere cells express five major integrins, alpha5 beta1, alpha 6Abeta1, alphav beta1, alphav beta5 and alpha vbeta8 and, in addition, express low levels of alpha 6Bbeta1. Chain migration is inhibited by blocking the alpha 6beta1 integrin. Proliferation, by contrast, is inhibited by blocking the other beta1 integrins, alphav beta1 and alpha5 beta1. These results show that integrins are important regulators of neural precursor cell behaviour, with distinct beta1 integrins regulating proliferation and migration. They also demonstrate a novel role for the alpha6 beta1 integrin in the cell-cell interactions underlying homotypic chain migration.
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Cerenak, Andreja, Zlatko Satovic, and Branka Javornik. "Genetic mapping of hop (Humulus lupulus L.) applied to the detection of QTLs for alpha-acid content." Genome 49, no. 5 (May 1, 2006): 485–94. http://dx.doi.org/10.1139/g06-007.

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The map locations and effects of quantitative trait loci (QTLs) were estimated for alpha-acid content in hop (Humulus lupulus L.) using amplified fragment length polymorphism (AFLP) and microsatellite marker (simple sequence repeat (SSR)) genetic linkage maps constructed from a double pseudotestcross. The mapping population consisted of 111 progeny from a cross between the German hop cultivar 'Magnum', which exhibits high levels of alpha-acids, and a wild Slovene male hop, 2/1. The progeny segregated quantitatively for alpha-acid content determined in 2002, 2003, and 2004. The maternal map consisted of 96 markers mapped on 14 linkage groups defining 661.90 cM of total map distance. The paternal map included 70 markers assigned to 12 linkage groups covering 445.90 cM of hop genome. QTL analysis indicated 4 putative QTLs (alpha1, alpha2, alpha3, and alpha4) on linkage groups (LGs) 03, 01, 09, and 03 of the female map, respectively. QTLs explained 11.9%–24.8% of the phenotypic variance. The most promising QTL to be used in marker-assisted selection is alpha2, the peak of which colocated exactly with the AFLP marker. Three chalcone synthase-like genes (chs2, chs3, and chs4) involved in hop bitter acid synthesis mapped together on LG04 of the female map. Saturation of the maps, particularly the putative QTL regions, will be carried out using SSR markers, and the stability of the QTLs will be tested in the coming years.Key words: Humulus lupulus L., genetic maps, alpha-acid content, QTLs.
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Aramini, J. "NMR solution structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its unmodified control." Nucleic Acids Research 26, no. 24 (December 15, 1998): 5644–54. http://dx.doi.org/10.1093/nar/26.24.5644.

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Newberg, M. H., D. H. Smith, S. B. Haertel, D. R. Vining, E. Lacy, and V. H. Engelhard. "Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules." Journal of Immunology 156, no. 7 (April 1, 1996): 2473–80. http://dx.doi.org/10.4049/jimmunol.156.7.2473.

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Abstract The importance of the species of different domains of class I MHC molecules in peripheral T cell recognition and positive and negative selection was evaluated in a single system. In transgenic mice expressing AAD (containing the alpha1+alpha2 domains of HLA-A2.1 and the alpha3 domain of H-2Dd), the CTL response to influenza peptide M1(58-66) in the context of the alpha1+alpha2 domains of HLA-A2.1 was as strong as the influenza-specific H-2Db-restricted response. However, this strong response was only discernible if the target cell MHC molecule also contained a murine alpha3 domain. In contrast, the response in HLA-A2.1 transgenic mice was about 30-fold weaker, and these CTL were indifferent to the origin of the target molecule alpha3 domain. Further analysis suggested that the major impact of the murine alpha3 domain of the transgene product was to enhance positive selection of a low affinity population of AAD-restricted T cells, presumably through species-specific interaction with CD8. Surprisingly, the response to non-self human class I MHC determinants was not augmented in AAD mice, indicating that the T cells selected are narrowly focused on AAD-related structures. Further analysis indicated that the alphal+alpha2 domains as well as the alpha3 domain influenced the magnitude of the response to non-self human class I MHC determinants, and this effect was mapped to alpha2. We suggest that the alpha2 domains of murine class I molecules contain conserved structural elements that augment the avidity of T cell-class I interactions, and this is particularly important in the recognition of non-self MHC molecules.
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Caniggia, I., J. Liu, R. Han, J. Wang, A. K. Tanswell, G. Laurie, and M. Post. "Identification of receptors binding fibronectin and laminin on fetal rat lung cells." American Journal of Physiology-Lung Cellular and Molecular Physiology 270, no. 3 (March 1, 1996): L459—L468. http://dx.doi.org/10.1152/ajplung.1996.270.3.l459.

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Fibronectin and laminin have been implicated in regulating lung morphogenesis. In the present study, the cell surface receptors involved in fetal lung cell binding to laminin and fibronectin were identified. Messages for alpha5- and beta1-integrin subunits were detected in both fetal lung epithelial cells and fibroblasts. The presence of alpha5 beta1 -integrin on both cell types was demonstrated by immunocytochemistry and confirmed by cell adhesion experiments with fibronectin and RGD-containing peptides. Epithelial cells adhered more readily to laminin than fibroblasts. The alpha4 beta1-integrin, and RGD-independent fibronectin receptor, was weakly expressed on either cell type. Both cell types expressed alpha6-integrin subunit mRNA and stained immunopositive for the alpha6-subunit. Although either cell type expressed nonintegrin 67-kDa laminin-elastin receptor mRNA, no positive immunoreactivity for this laminin-elastin binding protein was detected. None of these findings explain the enhanced attachment of distal fetal lung epithelial cells to laminin compared with fibroblasts. Previously, we have reported that epithelial cells were enriched in alpha3-integrin subunit mRNA and protein expression. Herein, we found that epithelial cell attachment to laminin was nearly completely inhibited by alpha3- but only partially by alpha6 -monoclonal antibodies. A peptide near the globular region at the long arm of the laminin A-chain, which contained the IKVAV sequence, and the laminin A-chain amino acid sequence representing the alpha3 beta1 -integrin binding site, inhibited the adherence of epithelial cells to laminin. Fetal lung epithelial cells attached to substrata coated with the alpha3 beta1-integrin binding site peptide and the peptide containing the IKVAV sequence. These data suggest that both fetal lung cell types bind to fibronectin via the fibronectin receptor, alpha5 beta1, and fetal lung epithelial cells interact with laminin via alpha3 beta1 and proteins that recognize the IKVAV-containing sequence on the laminin A-chain.
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Chabot, V., T. Magallon, C. Taragnat, and Y. Combarnous. "Two free isoforms of ovine glycoprotein hormone alpha-subunit strongly differ in their ability to stimulate prolactin release from foetal pituitaries." Journal of Endocrinology 164, no. 3 (March 1, 2000): 287–97. http://dx.doi.org/10.1677/joe.0.1640287.

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alpha-Subunit dissociated from glycoprotein hormones has been previously shown to stimulate rat pituitary lactotroph differentiation and proliferation. However, whether the free form of the alpha-subunit (free alpha) can also play such a role is not known. To test whether free alpha may act on prolactin (PRL) release from ovine foetal pituitaries, this molecule was purified and two major isoforms, alphaA and alphaB were isolated. Free alphaA was found to be more acidic and more hydrophobic than both free alphaB and ovine LH alpha-subunit (oLHalpha). Free alphaA and oLHalpha exhibited a molecular mass of 14 kDa as determined by mass spectrometry, whereas free alphaB displayed a molecular mass of only 13.5 kDa because of its truncated N-terminus. All three alpha molecules bear mature-type N-linked saccharide chains including Nacetyl galactosamine residues but none of them contains O-linked oligosaccharide. The free alphaA isoform, more than the oLHalpha, was able to stimulate PRL release from ovine foetal pituitary explants in culture, whereas the free alphaB isoform displayed no activity. Moreover, the free alphaA and alphaB isoforms were able to recombine with the ovine LH beta-subunit (oLHbeta). The free alphaB/oLHbeta, and the oLHalpha/oLHbeta dimer were 4-fold more active than the free alphaA/oLHbeta dimer in a specific LH radioreceptor assay and in the stimulation of testosterone release from rat Leydig cells. The present study demonstrates that the two free alpha isoforms of ovine glycoprotein hormones exhibit distinct efficiencies in stimulating PRL release from ovine foetal pituitaries. Moreover, despite their identical ability to recombine with the oLHbeta, the free alpha isoform, which is the most efficient on PRL release, is the least efficient in conferring LH activity on the alpha/beta dimer.
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Leivo, I., T. Tani, L. Laitinen, R. Bruns, E. Kivilaakso, V. P. Lehto, R. E. Burgeson, and I. Virtanen. "Anchoring complex components laminin-5 and type VII collagen in intestine: association with migrating and differentiating enterocytes." Journal of Histochemistry & Cytochemistry 44, no. 11 (November 1996): 1267–77. http://dx.doi.org/10.1177/44.11.8918902.

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Anchoring complex component laminin-5 and its subunits laminin (Ln)-alpha3 and Ln-beta3 chains, Type VII collagen, and integrin chains alpha3, alpha6, and beta4 were studied in developing and adult human intestine and compared with findings on Ln-alpha1 and Ln-alpha2 chains. In adult human duodenum, jejunum, and ileum, Ln-5 detected with a polyclonal antiserum and Ln-alpha3 and Ln-beta3 chains, detected with monoclonal antibodies (MAbs), were restricted to the epithelial basement membranes (BMs) of villi, whereas Ln-alpha2 chain was seen only focally in crypt bottoms. In double labeling experiments, the stretch of crypt BM corresponding to the proliferative cell compartment was found to be devoid of both Ln-alpha3 and Ln-alpha2 chains. Double labeling for Ln-5 and proliferating cell nuclear antigen also showed an abrupt onset of Ln-5 expression exactly at the upper edge of the proliferative cell compartment. Type VII collagen was negligible in duodenum and showed a rising duodenal-ileal gradient localizing to villar BMs. Double labeling for Ln-5 and Type VII collagen, however, indicated only partial co-distribution in the intestine. Electron microscopy of ileum revealed both anchoring filaments and anchoring fibrils but no hemidesmosomal plaques. Our results demonstrate the expression of Ln-5 in BMs outside of stratified epithelia and indicate that Ln-5 in the intestine is associated with the compartment of migrating and differentiating enterocytes. Absence of hemidesmosomes and the presence of other anchoring complex components, such as Ln-5, Type VII collagen, and integrin chains alpha3, alpha6, and beta4, suggests unique properties for epithelial cell attachment in the intestine.
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Dissertations / Theses on the topic "Alphat"

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Burton, Darren. "Searches for supersymmetric signatures in all hadronic final states with the alphaT variable." Thesis, Imperial College London, 2014. http://hdl.handle.net/10044/1/25065.

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A search for supersymmetric particles in events with high transverse momentum jets and a large missing transverse energy signature is con- ducted using 11.7 fb of data, collected with a center-of-mass collision energy of 8 TeV by the CMS detector. The dimensionless kinematic variable alphaT is used to select events with genuine missing transverse energy signatures. Standard Model backgrounds are estimated through the use of data driven control samples. No excess over Standard Model expectations is found. Exclusion limits on squark and gluino masses are set at the 95% confidence level in the parameter space of a range of supersymmetric simplified models. Results of benchmarking the Level-1 (the first line of the CMS trigger system) single jet and hadronic transverse energy trigger efficiencies, before and after the implementation of a change to the Level-1 jet clustering algorithm are presented. This was introduced to negate an increase in trigger cross-section, which can be attributed to soft jets from secondary interactions. Similar performance is observed for all L1 quantities before and after this change. Furthermore, a templated method to estimate the Standard Model background distribution of the number of jets originating from a b-quark within a supersymmetric search is validated in data and simulation. Applicable to searches sensitive to gluino induced third-generation signatures, this technique is utilised as a crosscheck to the results of the alphaT analysis. Standard Model background predictions from the template fits are compared to those from the alphaT search in the hadronic signal region, where good agreement between the two methods is observed.
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Spies, Markus. "Nikotinische Rezeptoren nozizeptiver Neurone : Lokalisation und Charakterisierung der {[alpha]3- [Alpha3-] und {[alpha]5-Untereinheiten [Alpha5-Untereinheiten] /." Giessen : VVB Laufersweiler, 2007. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=016297581&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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Rogerson, Samuel. "A search for supersymmetry using the alphaT variable with the CMS detector and the impact of experimental searches for supersymmetry on supersymmetric parameter space." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/18398.

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A search for supersymmetry in final states with jets and missing transverse energy is performed in pp collisions at a centre-of-mass energy of sqrt(s)=7TeV. The data sample corresponds to 4.98fb^-1 collected by the CMS experiment at the LHC in 2011. A dimensionless kinematic variable is used as the main discriminant between genuine and misreconstructed signal events. The search is performed in a signal region binned according to the scalar sum of the transverse energy of jets and the number of jets identified as originating from a bottom quark. The limits are presented in the parameter space of the cMSSM as well as in simplified models with particular attention paid to compressed spectra and third-generation models. Global frequentist fits to the cMSSM} and a non-universal Higgs model are also performed using the Mastercode framework incorporating recent experimental constraints, similar to those those presented here. Global likelihood contours are presented in the parameter planes of both the cMSSM and NUHM1, as well as a selection of 1D likelihood functions for observables.
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Ochoa, Vanessa. "Nicotinic Signaling: Alpha3 Beta4 Heteromers, Alpha5 Subunits, And The Prototoxin Lypd6b." ScholarWorks @ UVM, 2015. http://scholarworks.uvm.edu/graddis/472.

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Prototoxin proteins have been identified as members of the Ly6/uPAR super family whose three-finger motif resembles that of α-bungarotoxin. Though they are known to modify the function of nAChRs, their specificity is still unclear. Our lab identified three prototoxin proteins in the chicken ciliary ganglion: Ch3ly, Ch5ly, and Ch6ly. Ch6ly was later identified as prostate stem cell antigen (PSCA), and specifically decreased the amount of calcium influx through the homomeric α7 nAChR subtype. I then identifiedCh3ly and Ch5ly as LY6E and LYPD6B, respectively. I focused my attention onLYPD6B because of its expression in the brain. This dissertation tests whether LYPD6Bis a prototoxin protein that specifically co-localizes with and modifies the function of the heteromeric α3β4* nAChRs (the other nAChR subtype expressed in the chicken ciliary ganglia). In the first part of my dissertation I performed intracellular two-electrode voltage clamp on Xenopus oocytes co-expressing human LYPD6B and different stoichiometries of the α3β4* nAChR, these included two (α3)2(β4)3 withβ4−α3−β4−β4−α3 and β4−α3−β4−α3−β4 stoichiometries, two (α3)3(β4)2 with stoichiometries β4−α3−α3−β4−α3 and β4−α3−β4−α3−α3, two (α3β4)2(α5D)β4−α3−α5D−β4−α3 and β4−α3−β4−α3−α5D, and (α3β4)2(α5N) with stoichiometries β4−α3−α5N−β4−α3 and β4−α3−β4−α3−α5N. Concatemeric constructs are designed to link nAChR subunits, thus when translated it is done so as a single polypeptide. LYPD6Bincreased the acetylcholine (ACh) potency and desensitization rate, but decreased the maximum current response (Imax) for the (α3)3(β4)2 nAChR subtype. Yet, LYPD6Bonly decreased the Imax for the (α3β4)2α5 D-variant and not the N-variant (associated with increase nicotine consumption). For the second part of my dissertation, I determined if the expression of LYPD6B correlated with nAChRs in an activity dependent manner. Though LYPD6B mRNA expression correlates with nAChR subunit mRNA expression levels, it seemed to be independent of nAChR activity. To determine if fluorescent colocalization occurs between LYPD6B and a specific nAChR subtype, I genetically engineered LYPD6B to express a human influenza hemagglutinin (HA) epitope tag and cloned into a chicken retrovirus. LYPD6B was shown to co-localize only with the α3β4*heteromeric and not the homomeric α7 nAChRs, in a nAChR activity dependent manner. This study adds to the complexity of a prototoxin’s function by suggesting that the specificity is dependent on nAChR type and stoichiometry. It is the first in identifying a prototoxin protein, LYPD6B, which specifically modulates the function of the(α3)3(β4)2 and (α3β4)2(α5 D-variant) heteromeric nAChR subtypes. For the (α3β4)2(α5D-variant) nAChR subtype LYPD6B decreased the Imax. Such observation may be telling of a novel mechanism involved with nicotine dependence. For the(α3)3(β4)2 nAChR subtype LYPD6B increases its ACh sensitivity, desensitization rate, while decreasing Imax. Additionally, the co-localization of LYPD6B and α3β4* nAChRsin the lack of nAChR activity highlights the relevance of the functional effects α3β4*nAChRs exhibit due to LYPD6B. Such relevance may be the utilization of limiting Ach amounts.
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Xu, Xiaohong. "Shared long-range regulatory elements coordinate expression on the nAChR beta4/alpha3/alpha5 cluster." Connect to text online, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=case1157660172.

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Xu, Xiaohong. "SHARED LONG-RANGE REGULATORY ELEMENTS COORDINATE EXPRESSION OF THE NACHR BETA4/ALPHA3/ALPHA5 CLUSTER." Case Western Reserve University School of Graduate Studies / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=case1157660172.

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Schäfer, Heike. "Untersuchung der Proteinalterung am Beispiel des [alpha]A-Crystallins [alphaA-Crystallins] der Augenlinse mittels proteomanalytischer Methoden." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972090770.

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Ponchel, Guillaume. "Etudes structurales et fonctionnelles de deux alpha-galactosidases actives sur les antigènes B et alpha3 Gal." Thesis, Aix-Marseille, 2012. http://www.theses.fr/2012AIXM4017.

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Les conversions enzymatiques des antigènes du système des groupes sanguins ABO et de l'antigène majeur de xénotransplantation α3Gal représentent des alternatives thérapeutiques afin d'améliorer l'approvisionnement des hôpitaux en sang de groupe O et la transplantation d'organes. La famille GH110 des glycoside hydrolases comprend des enzymes actives sur les antigène B et α3Gal à pH neutre et fonctionnant avec un mécanisme catalytique par inversion. La sous‐famille GH110_A regroupe des membres actifs exclusivement sur l'antigène B, tandis que la sous‐famille GH110_B regroupe des membres actifs sur les antigènes B et α3Gal. Le travail présenté dans ce manuscrit décrit les études structurales et fonctionnelles de deux α‐galactosidases, BtGal110A (GH110_A) et BtGal110B (GH110_B), de la famille GH110 provenant de la bactérie commensale Bacteroides thetaiotaomicron et représentatives des chacune des ces deux sous‐familes. La spécificité de substrat et la grande efficacité catalytique de ces enzymes à pH neutre ont été confirmées grâce à des oligosaccharides mimant l'extrémité des antigènes B et α3Gal. BtGal110A et BtGal110B se replient en hélice β droite et se distinguent d'autres protéines au repliement similaire par la présence de deux domaines additionnels qui adoptent un repliement en demi tonneau β et participent à l'architecture du site actif. La machinerie catalytique de BtGal110A et BtGal110B est constituée de trois acides aspartiques, semblable à celle des enzymes des familles GH28 et GH49, qui partagent la même repliement en hélice &#946
Enzymatic conversions of the ABO blood group antigens and of the major xenotransplantation α3Gal antigen represent therapeutic alternatives intended to improve supply of hospitals with requested blood type O and organs. The family GH110 of glycoside hydrolases includes enzymes active towards B and α3Gal antigen within neutral pH and employs an inverting catalytic mechanism. The GH110_A subfamily gathers enzymes with exquisite substrate specificity towards the B antigen, while the GH110_B subfamily gathers enzymes active towards the B and the α3Gal antigens. The present manuscript describes the functional and structural studies of two α‐galactosidases BtGal110A (GH110_A) and BtGal110B (GH110_B) from the commensal bacteria Bacteroides thetaiotaomicron, two representative members of each of these two subfamilies. The substrate specificity and high catalytic efficiency of these enzymes at neutral pH were confirmed using oligosaccharides corresponding to the terminal carbohydrate sequences of the B and α3Gal antigens. BtGal110A and BtGal110B contain a right‐handed β‐helix, but he presence of two additional domains tfolded as half β‐barrels, which participate to the architecture of the active site distinguishes BtGal110A and BtGal110B fro other related glycoside hydrolases. The catalytic machinery consists of three aspartic acids, reminiscent of that of enzymes from the GH28 and GH49 families, which share a similar β‐helix fold. Our observations support the membership of family GH110 to the clan‐N of glycoside hydrolases
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Solda', G. M. E. A. "Alpha3 and alpha5 neuronal nicotinic receptor subunit genes : a case of tail-to-tail overlap in humans." Doctoral thesis, Università degli Studi di Milano, 2005. http://hdl.handle.net/2434/47108.

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Natural antisense transcripts, because of their potential to form double-stranded RNA (dsRNA) molecules, recently emerged as a mechanism acting on eukaryotic gene regulation at multiple levels. CHRNA3 and CHRNA5, coding for alpha3 and alpha5 subunits of the neuronal nicotinic acetylcholine receptor, have been reported to overlap at their 3'ends in human and bovine genomes. The general goal of this PhD project was the structural and functional study of this overlap. A fine characterization of human CHRNA3 and CHRNA5 alternative transcripts allowed a more precise definition of the overlap extent and highlighted a complex web of possible sense-antisense interactions among overlapping mRNAs. An RNase protection-based approach was used to detect in vivo RNA-RNA duplexes of CHRNA3 and CHRNA5 transcripts, which resulted to be present in human but not in bovine cells. Furthermore, levels of overlapping transcripts were determined by real-time RT-PCR both in humans and in cattle. Finally, some possible functional consequences of this overlap were explored.
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Gräf, Thorsten. "Hemmung der Angiogenese in vitro durch Antisense-Oligonukleotide gegen die [alpha]v-Kette [alphav-Kette] des Vitronectin-Rezeptors." [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=970795068.

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Books on the topic "Alphat"

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Carter, David A. Alpha bugs: A pop-up alphabet. London: Orchard Books, 1994.

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Alpha bugs: A pop-up alphabet. New York: Little Simon, 1994.

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Man, John. Alpha beta: How our alphabet changed the western world. London: Headline, 2000.

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Hawley, Francesca. Alpha vs. alpha. Akron: Ellora's Cave Publishing Inc., 2011.

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Ludolph-Hauser, Dagmar. Einfluss von Inhibitoren der de novo N-Glykosylierung und des Oligosaccharid-"Processings" auf Biosynthese und Sekretion der hepatischen Glykoproteine [alpha]1-Proteinase [Alpha1-Proteinase] Inhibitor und [alpha]1-Saures [Alpha1-Saures] Glykoprotein. [s.l.]: [s.n.], 1992.

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Kagaku Busshitsu Hyōka Kenkyū Kikō and Shin Enerugī Sangyō Gijutsu Sōgō Kaihatsu Kikō (Japan), eds. [Alpha]-mechirusuchiren: [Alpha]-Methylstyrene. Tōkyō: Seihin Hyōka Gijutsu Kiban Kikō Kagaku Busshitsu Hyōka Kenkyū Kikō, 2009.

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(Museum), Animation-recherche-confrontation, ed. Alpha. Zürich: JRP/Ringier, 2005.

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Arsenault, Isabelle. Alpha. Somerville, Massachusetts: Candlewick Press, 2015.

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Anderson, Felix. Alpha among Alphas. Primedia eLaunch LLC, 2022.

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Valerie. Alpha's Alphabet. Westwood Books Publishing, 2022.

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Book chapters on the topic "Alphat"

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Bunce, Pauline. "12. The English Alphabet: Alpha-Best or Alpha-Beast?" In Why English?, edited by Pauline Bunce, Robert Phillipson, Vaughan Rapatahana, and Ruanni Tupas, 142–53. Bristol, Blue Ridge Summit: Multilingual Matters, 2016. http://dx.doi.org/10.21832/9781783095858-015.

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Tulchinsky, Igor. "Introduction to Alpha Design." In Finding Alphas, 1–5. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119057871.ch1.

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Yan, Peng. "Backtest - Signal or Overfitting." In Finding Alphas, 55–59. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119057871.ch10.

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Wan, Peng. "Alpha and Risk Factors." In Finding Alphas, 61–64. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119057871.ch11.

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Aron, Ionut. "The Relationship between Alpha and Portfolio Risk." In Finding Alphas, 65–70. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119057871.ch12.

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Khan, Hammad. "Risk and Drawdowns." In Finding Alphas, 71–77. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119057871.ch13.

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Li, Weijia. "Data and Alpha Design." In Finding Alphas, 79–83. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119057871.ch14.

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Fang, Zhuangxi. "Statistical Arbitrage, Overfitting, and Alpha Diversity." In Finding Alphas, 85–87. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119057871.ch15.

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Kozlov, Michael. "Techniques for Improving the Robustness of Alphas." In Finding Alphas, 89–91. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119057871.ch16.

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Huang, Yu. "Alphas from Automated Search." In Finding Alphas, 93–95. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119057871.ch17.

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Conference papers on the topic "Alphat"

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Dittmaier, Stefan, Alexander Huss, and Christian Schwinn. "O(alpha*alphas) corrections to Drell-Yan processes in the resonance region." In 11th International Symposium on Radiative Corrections (Applications of Quantum Field Theory to Phenomenology). Trieste, Italy: Sissa Medialab, 2014. http://dx.doi.org/10.22323/1.197.0020.

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Webster, Jeffrey A., Alexander Hagen, Brian C. Archambault, Nicholas Hume, and Rusi Taleyarkhan. "High Efficiency Gamma-Beta Blind Alpha Spectrometry for Nuclear Energy Applications." In 2014 22nd International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/icone22-30821.

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A novel, Centrifugally Tensioned Metastable Fluid Detector (CTMFD) sensor technology has been developed over the last decade to demonstrate high selective sensitivity and detection efficiency to various forms of radiation for wide-ranging conditions (e.g., power level, safeguards, security, and health physics) relevant to the nuclear energy industry. The CTMFD operates by tensioning a liquid with centrifugal force to weaken the bonds in the liquid to the point whereby even a femto-scale nuclear particle interactions can break the fluid and cause a detectable vaporization cascade. The operating principle has only peripheral similarity to the superheated bubble chamber based superheated droplet detectors (SDDs); instead, CTMFDs utilize mechanical “tension pressure” instead of thermal superheat offering a lot of practical advantages. CTMFDs have been used to detect a variety of alpha and neutron emitting sources in near real-time. The CTMFD is selectively blind to gamma photons and betas allowing for detection of alphas and neutrons in extreme gamma/beta background environments such as spent fuel reprocessing plants or under full power conditions within an operating nuclear reactor itself. The selective sensitivity allows for differentiation between alpha emitters including the isotopes of Plutonium. Mixtures of Plutonium isotopes have been measured in ratios of 1:1, 2:1, and 3:1 Pu-238:Pu-239 with successful differentiation. Due to the lack of gamma-beta background interference, the CTMFD’s LLD can be effectively reduced to zero and hence, is inherently more sensitive than scintillation based alpha spectrometers or SDDs and has been proven capable to detect below femtogram quantities of Plutonium-238. Plutonium is also easily distinguishable from Neptunium making it easy to measure the Plutonium concentration in the NPEX stream of a UREX reprocessing facility. The CTMFD has been calibrated for alphas from Americium (5.5 MeV) and Curium (∼6 MeV) as well. The CTMFD has furthermore, recently also been used to detect spontaneous and induced fission events which can be differentiated from alpha decay allowing for detection of fissionable material in a mixture of isotopes. This paper discusses these transformational developments which are also being entered for real-world commercial use.
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Puinean, Mirel. "Allosteric modulation of tick nicotinic acetylcholine receptor alpha 5 and alpha6 by spinosad." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.91232.

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Progri, Ilir F. "Description of VBOC2(alpha1,alpha2) GMGM Special Cases Waveforms ACF---Theory, Computation, Simulations, and Animation." In 2020 International Technical Meeting of The Institute of Navigation. Institute of Navigation, 2020. http://dx.doi.org/10.33012/2020.17212.

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Johnson, Jeremy, Piotr Richahou, and B. Mark Evers. "Abstract 93: AMPK alpha1 and AMPK alpha 2 control TNBC proliferation through cell cycle regulation." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-93.

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Dittmaier, Stefan, Alexander Huss, and Christian Schwinn. "O(alpha_s alpha) corrections to Drell-Yan processes in the resonance region." In Loops and Legs in Quantum Field Theory. Trieste, Italy: Sissa Medialab, 2014. http://dx.doi.org/10.22323/1.211.0045.

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Rzehak, Heidi, Thomas Hahn, Sven Heinemeyer, Wolfgang Hollik, and Georg Ralf Weiglein. "The Higgs sector in the CP-violating MSSM at ${\cal O}(\alpha_t\alpha_s)$." In 8th International Symposium on Radiative Corrections. Trieste, Italy: Sissa Medialab, 2008. http://dx.doi.org/10.22323/1.048.0044.

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Mysliwiec, M., D. Alderson, L. Poller, and P. Ackrill. "PROTEIN C AND OTHER CLOTTING STUDIES IN MEMBRANOUS AND NON-MEMBRANOUS GLOMERULONEPHRITIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644310.

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The occurrence of thrombosis in the nephrotic syn drome has long been known. Thrombotic complications are predominantly associated with membranous glomerulonephritis (MG). The aim of the present work was to study whether the tendency of nephrotic patients with MG to thrombotic episodes could be attributed to a hypercoagulable state. Thirty consecutive patients with the nephrotic syndrome were studied. Of these 17 suffered from MG and 13 had other forms of glomerulonephritis. The control group consisted of 10 healthy volunteers. In addition to standard coagulation assays, we studied: soluble fibrin monomer complexes (FM test, Boehringer), fibrin monomer polymerization, factor VIII:C, factor VIII:vWF, anti thrombin III (AT III) and alpha2 antiplasmin (alpha2AP) using chromogenie substrates; the levels of AT III and alpha2 AP were measured immunologically; beta thromboglobulin (BTG), platelet factor 4 and fibrinopeptide A (FPA) using radioimmunoassay kits; protein C was studied functionally and immunologically. There was a significant shortening of the prothrombin time and activated partial thromboplastin time, increase in alpha9 AP, factor V111:vWF, FPA and BTG in nephrotic patients associated with in or eases in both functional and imminclogical protein C levels and impairment of fibrin polymerization. FM test was negative in all but one of the patients. None of the coagulation tests showed a significant difference in the two nephrotic groups. High protein C and impaired polymerization may be considered as mechanisms counteracting disclosed hypercoagulability in the nephrotic syndrome.
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Batalla Toro, Luis F., Simon L. Reid, Alfredo Salcines Tudela, and Duncan Graham. "Integrated Decommissioning of a Pentagon Shaped Production Semisubmersible in the UK: A Unique Challenge." In ASME 2018 37th International Conference on Ocean, Offshore and Arctic Engineering. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/omae2018-77831.

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Between 1969 and 1977, eleven semisubmersible drilling platforms were designed and built with an innovative pentagon shaped hull, specifically to work in the harsh environment of the North Sea. One of the drilling rigs, the Alexander L. Kielland, was converted soon after construction into an accommodation platform (flotel) and failed catastrophically in 1980. Another, the Pentagon 83 “Drillmaster” (renamed as Buchan Alpha), was being converted to a Floating Production Unit at the time of the disaster. The structure of Buchan Alpha was significantly modified during the conversion of the platform so that it benefited from the lessons learnt following the Alexander L. Kielland accident to ensure that the same sequence of events could not be repeated. This technical paper objective is to explain the integrated decommissioning process of the Buchan Alpha in the UK after more than 40 years since being built and more than 35 years of successful operation since it was converted to a Floating Production Unit, and how the features of its original design have accompanied the platform through the decommissioning process. The scope covers all phases of Buchan Alpha decommissioning from the detailed planning and preparation, the suspension of production up to the dismantling and recycling process. Significant challenges for the decommissioning team included the requirement to preserve the operational status of the subsea infrastructure for potential future field redevelopment and the diver disconnection of the subsea wells. Buchan Alpha’s deep draught presented limitations on the selection of dismantling and recycling yards due to quayside water depths. Complex ballasting operations and removal of the thruster’s propellers were required to facilitate the platform berthing at the quayside. Key lessons learned applicable for future decommissioning of floating production facilities will be shared.
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Ramos, Alberto, M. Bruno, Mattia Dalla Brida, P. Fritzsch, T. Korzec, S. Schaefer, H. Simma, Stefan Sint, and Rainer Sommer. "The $\Lambda$-parameter in 3-flavour QCD and $\alpha_s(m_Z)$ by the ALPHA collaboration." In 34th annual International Symposium on Lattice Field Theory. Trieste, Italy: Sissa Medialab, 2017. http://dx.doi.org/10.22323/1.256.0197.

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Reports on the topic "Alphat"

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Ashman, William P., and Alexander P. Mickiewicz. Computer-Assisted Determination of Minimum Energy Conformations. 4. Alpha1 and Alpha2 Adrenergic Compounds. Fort Belvoir, VA: Defense Technical Information Center, June 1990. http://dx.doi.org/10.21236/ada226811.

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Бас, Світлана Віталіївна, and Катерина Іванівна Словак. Способи опрацювання запитів та характеристика мобільного доступу до Wolfram|Alpha. Видавничий центр ДВНЗ «Криворізький національний університет», December 2014. http://dx.doi.org/10.31812/0564/1081.

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У статті проаналізовано способи опрацювання запитів різних видів до Wolfram|Alpha, зокрема, можливості запитів природною мовою. Надано характеристику мобільному доступу до Wolfram|Alpha, розглянуто формати подання даних: візуальні подання, зображення, HTML, Mathematica Cell, текстові подання, простий текст, MathML, введення Mathematica, виведення Mathematica, audio подання, спеціальні типи виводу Wolfram|Alpha. Метою статті є аналіз та узагальнення можливостей подання запитів до Wolfram|Alpha різними способами та надання характеристики можливостей мобільного доступу до Wolfram|Alpha. Предметом дослідження є Wolfram|Alpha як хмаро орієнтований сервіс навчання математики. При підготовці матеріалу було використано тематичні дослідження та експериментальне дослідження можливостей подання різних типів запитів до Wolfram|Alpha. Висновок: робота Wolfram|Alpha заснована на опрацюванні природної мови (поки тільки англійської), великій бібліотеці алгоритмів і NKS-підході до відповідей на запити. Wolfram|Alpha не видає перелік посилань, що ґрунтується на результатах запиту, а обчислює відповідь, ґрунтуючись на власній базі знань. Сервіс здатен перекладати дані між різними одиницями вимірювання, системами числення тощо.
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Kwun, Hegoen, and Sang Kim. LY9DLGF High-Power Long-Range Guided-Wave Inspection of Pipelines. Chantilly, Virginia: Pipeline Research Council International, Inc. (PRCI), July 2005. http://dx.doi.org/10.55274/r0012077.

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The objective of this project was to increase the guided wave power of the magneto-strictive sensor (MsS) technology so that the test range of guided wave achievable on pipelines could be further extended. The target goal of the project was a 20-fold (or 26-dB) increase in the guided-wave signal amplitude. The extension in the test range attainable by the 20-fold increase in the guided-wave signal amplitude depends on the wave attenuation coefficient, and alpha;, of the pipe-line under testing and is equal to 26/(2 and alpha;), where the factor 2 accounts for the round trip. For example, for and alpha; = 2.0 dB/ft, the extension in the test range is 6.5 feet; for and alpha; = 1.0 dB/ft, 13 feet; for and alpha; = 0.5 dB/ft, 26 feet; for and alpha; = 0.25 dB/ft, 52 feet; for and alpha; = 0.1 dB/ft, 130 feet; for and alpha; = 0.05 dB/ft, 260 feet.
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Хараджян, Наталя Анатоліївна, and Микола Анатолійович Слюсаренко. Використання web-сервісу Wolfram|Alpha при вивченні фізики. Видавничий центр ДВНЗ «Криворізький національний університет», 2017. http://dx.doi.org/10.31812/0564/1544.

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Метою дослідження є аналіз можливостей використання web-орієнтованої системи Wolfram|Alpha при вивченні фізики. Задачі: розглянути можливості використання web-орієнтованої системи Wolfram|Alpha при вивченні фізики. Об’єктом дослідження є використання систем комп’ютерної математики при вивченні фізики. Предметом дослідження є використання web-орієнтованих систем комп’ютерної математики в процесі вивчення фізики. Для досягнення цілей дослідження використовувалось кілька методів дослідження: аналіз науково-методичної літератури, присвяченій системам комп’ютерної математики, візуалізації отриманих результатів. Результатами дослідження є проаналізовані можливості використання web-орієнтованої системи Wolfram|Alpha при вивченні фізики. Висновки: Web-орієнтовану систему Wolfram|Alpha доцільно використовувати при вивченні фізики в якості допоміжного інструменту при розв’язанні задач, знаходження довідникових відомостей, візуалізації отриманих розв’язків і т.д.
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Ekdahl, Carl. Alpha. Office of Scientific and Technical Information (OSTI), December 2023. http://dx.doi.org/10.2172/2246824.

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Chen, Cheng-Che, and Hao-En Lin. Survival Benefits and Bleeding Risk of Anti-VEGF Agents for Renal Cell Carcinoma (RCC): A Updated Systematic Review and Meta-Analysis of Phase 2 and 3 Randomized Clinical Trials. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, March 2023. http://dx.doi.org/10.37766/inplasy2023.3.0007.

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Review question / Objective: To investigate the survival benefits (PFS, DFS, OS) and bleeding risk of the anti-VEGF agents compared with placebo or interferon alpha (IFNa) in patients with RCC. Condition being studied: Part 1. The hazard ratio (HR) of the progression-free survival (PFS) and overall survival (OS) of anti-VEGF agents vs. non/placebo for patients with unresectable, advanced, metastatic, renal cell carcinoma (RCC). Part 2. The HR of the disease-free survival (PFS) and OS of anti-VEGF agents vs. non/placebo for patients with post-nephrectomy RCC (adjuvant use). Part 3. The HR of the PFS and OS of anti-VEGF agents vs. IFN-alpha for patients with RCC. Part 4. The relative risk (RR) of bleeding events of anti-VEGF agents vs. placebo or IFN-alpha for patients with RCC.
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Scott, Cathy. Earning Alpha by Avoiding the Index Rebalancing Crowd (In Practice). CFA Institute, May 2023. http://dx.doi.org/10.56227/23.1.14.

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Barillas, Francisco, and Jay Shanken. Which Alpha? Cambridge, MA: National Bureau of Economic Research, November 2015. http://dx.doi.org/10.3386/w21698.

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Frazzini, Andrea, David Kabiller, and Lasse Pedersen. Buffett's Alpha. Cambridge, MA: National Bureau of Economic Research, November 2013. http://dx.doi.org/10.3386/w19681.

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Salmon, R., and O. Hermann. ALPHN: A computer program for calculating ([alpha], n) neutron production in canisters of high-level waste. Office of Scientific and Technical Information (OSTI), October 1992. http://dx.doi.org/10.2172/7147581.

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