Academic literature on the topic 'Aminex HPX-87H'

Abstract A rapid, high yield, and quantitative method for determination of sugars (glucose and fructose) and acids (malic, lactic, acetic, and citric) in fresh and fermented cucumber juices is described; the procedure solves the fructose and malic acid coelution problem. Samples are prepared by passing the juice directly through a disposable C18 cartridge. Compounds are separated by liquid chromatography on an Aminex HPX-87H chromatographic column, with 0.013N H2S04 as mobile phase in the isocratic mode at 0.6 mL/min flow rate and 60°C column temperature. Compounds are then detected and quantitated by using ultraviolet and refractive index detectors connected in series, and comparing with succinic acid as internal standard. Values for both fresh and fermented cucumber juices were in good agreement with literature data.

At present, inulin is often added to multivitamin syrup product. The determination of the component of preparation both qualitatively and quantitatively is important to ensure quality of the product. This research is aimed to develop a high performance liquid chromatography method to analyze inulin in multivitamin syrup preparation. Separation of inulin from the sample, was performed using Aminex column HPX-87H (300 x 7.8 mm) Ion Exclusion at a temperature of 80 °C with isocratic elution system using deionized water as mobile phase at a flow rate of 0.5 mL/min, and detected by using refractive index detector. This method validation showed a good linearity with correlation coefficient (r) of 0.999 while the coefficient of variation of the regression function (Vx0) was 2.00%. The limit of detection (LOD) and the limit of quantification (LOQ) of the method were respectively 0.12 mg/mL and 0.37 mg/mL. The mean absolute recovery of inulin from the simulation sample was 99.42% and the method precision was less than 2%. The proposed method has been applied to the determination of inulin in commercial multivitamin syrup and the recovery of label claim was 99.9 mg/100 mL. The proposed HPLC method is rapid, simple, and selective for routine analysis.

Abstract Three methods for the determination of glycerin are examined as applied to several allergenic extracts and diagnostic antigens. The liquid chromatographic procedure uses a sulfonic acid functional PSDVB resin (Aminex HPX-87H), a mobile phase of 0.013N H2S04; and refractive index detection. The titrimetric procedure involves oxidation of glycerin with sodium metaperiodate followed by potentiometric titration of the resulting formic acid with sodium hydroxide. Samples are quantitated by comparing the equivalence point obtained from the sample to those obtained from a series of standards. The gas chromatographic procedure includes a column of 5% Carbowax 20 M on 80-100 mesh Chromosorb WHP; p-cresol was used as an internal standard. The 3 procedures are shown to be valid for the majority of product types examined. A positive interference was encountered in the titrimetric analysis of a tuberculin purified protein derivative that contained simple sugars. Recoveries of added glycerin ranged from 95.0 to 100.2% by the liquid chromatographic method, from 98.7 to 101.4% by the gas chromatographic method, and from 99.8 to 101.6% by the metaperiodate oxidation method when interference from simple sugars was not present. Coefficients of variation determined from 8 replicates of samples that contained glycerin were 2.2% or less for the liquid chromatographic method, 2.3% or less for the GC method, and 3.6% or less for the metaperiodate oxidation method.

This thesis explored the development of a faster and more efficient means of qualitative and quantitative analysis of the products of the nitric acid oxidation of D-glucose and other simple sugars, for the Shafizadeh Rocky Mountain Center for Wood and Carbohydrate Chemistry. During the research, analysis was carried out based on previous work completed in a similar area using two Aminex HPX-87H+ cation-exchange columns at different temperatures, and plumbed in series. Standards were filtered and injected on to the columns, then eluted with 5 mM sulfuric acid. A total run time of 33 minutes enabled the elution of all products and by-products of the reaction. Retention times of standards and the use of spiking helped specify and quantify unknowns in samples from a series of oxidation reactions involving D-glucose and other aldoses. The PrevailTM Organic acid (OA) column was said to provide 'unsurpassed resolution of organic acids'. It was therefore investigated, and a method was developed and refined in order to optimise conditions enabling the column's use for the required analyses. The optimised parameters were established as: ambient temperature with an eluent of 10 mM KH2PO4 adjusted to a pH of 2.1 with phosphoric acid. The sample size was 5 uL with a flow rate of 0.3 mL/min, giving a total run time of approximately 13 minutes. The Aminex HPX-87H+ column method and the PrevailTM OA method were compared to determine the superior method for the analyses intended. While some improvements were made for detection in the PrevailTM OA method, results were not satisfactory. This was due in part to limits imposed on the PrevailTM OA column method, which prevented the use of gradient elution. The Aminex HPX-87H+ column method outlined herein provides superior resolution for the nitric acid oxidation of D-glucose to D-glucaric acid, and in conclusion it is suggested that the Aminex HPX-87H+ column method be used.