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Journal articles on the topic 'Amino Acid Sequence Homology'

Author: Grafiati

Published: 25 July 2024

Last updated: 31 July 2025

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1

Xia-Yun, Jiang, Zou Shu-Ming, and Zhou Pei-Gen. "Cloning and sequence analysis of complete cDNA of chitin deacetylase from Mucor racemosus." Chinese Journal of Agricultural Biotechnology 4, no. 2 (2007): 167–72. http://dx.doi.org/10.1017/s1479236207001489.

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AbstractA complete chitin deacetylase (CDA) complementary DNA (cDNA) from Mucor racemosus was cloned and sequenced by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification cDNA end (RACE) with gene special conserved primers. The cDNA sequence was submitted to GenBank (DQ538514). The complete cDNA with full-length of 1506 bp contained a 67 bp 5′-untranslated region, an open reading frame of 1344 bp and 95 bp 3′-untranslated region including tailing site AATAAA. The gene encoded a sequence of 448 amino acid residues and consisted of core nucleotides encoding a polysacc

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2

Reenan, R. A., and R. D. Kolodner. "Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins." Genetics 132, no. 4 (1992): 963–73. http://dx.doi.org/10.1093/genetics/132.4.963.

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Abstract Homologs of the Escherichia coli (mutL, S and uvrD) and Streptococcus pneumoniae (hexA, B) genes involved in mismatch repair are known in several distantly related organisms. Degenerate oligonucleotide primers based on conserved regions of E. coli MutS protein and its homologs from Salmonella typhimurium, S. pneumoniae and human were used in the polymerase chain reaction (PCR) to amplify and clone mutS/hexA homologs from Saccharomyces cerevisiae. Two DNA sequences were amplified whose deduced amino acid sequences both shared a high degree of homology with MutS. These sequences were th

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3

Saxena, A., R. Padmanabha, and C. V. Glover. "Isolation and sequencing of cDNA clones encoding alpha and beta subunits of Drosophila melanogaster casein kinase II." Molecular and Cellular Biology 7, no. 10 (1987): 3409–17. http://dx.doi.org/10.1128/mcb.7.10.3409-3417.1987.

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Cloned cDNAs encoding both subunits of Drosophila melanogaster casein kinase II have been isolated by immunological screening of lambda gt11 expression libraries, and the complete amino acid sequence of both polypeptides has been deduced by DNA sequencing. The alpha cDNA contained an open reading frame of 336 amino acid residues, yielding a predicted molecular weight for the alpha polypeptide of 39,833. The alpha sequence contained the expected semi-invariant residues present in the catalytic domain of previously sequenced protein kinases, confirming that it is the catalytic subunit of the enz

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4

Saxena, A., R. Padmanabha, and C. V. Glover. "Isolation and sequencing of cDNA clones encoding alpha and beta subunits of Drosophila melanogaster casein kinase II." Molecular and Cellular Biology 7, no. 10 (1987): 3409–17. http://dx.doi.org/10.1128/mcb.7.10.3409.

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Cloned cDNAs encoding both subunits of Drosophila melanogaster casein kinase II have been isolated by immunological screening of lambda gt11 expression libraries, and the complete amino acid sequence of both polypeptides has been deduced by DNA sequencing. The alpha cDNA contained an open reading frame of 336 amino acid residues, yielding a predicted molecular weight for the alpha polypeptide of 39,833. The alpha sequence contained the expected semi-invariant residues present in the catalytic domain of previously sequenced protein kinases, confirming that it is the catalytic subunit of the enz

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5

Day, A. A., C. I. McQuillan, J. D. Termine, and M. R. Young. "Molecular cloning and sequence analysis of the cDNA for small proteoglycan II of bovine bone." Biochemical Journal 248, no. 3 (1987): 801–5. http://dx.doi.org/10.1042/bj2480801.

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The cDNA for the full-length core protein of the small chondroitin sulphate proteoglycan II of bovine bone was cloned and sequenced. A 1.3 kb clone (lambda Pg28) was identified by plaque hybridization with a previously isolated 1.0 kb proteoglycan cDNA clone (lambda Pg20), positively identified previously by polyclonal and monoclonal antibody reactivity and by hybrid-selected translation in vitro [Day, Ramis, Fisher, Gehron Robey, Termine & Young (1986) Nucleic Acids Res. 14, 9861-9876]. The cDNA sequences of both clones were identical in areas of overlap. The 360-amino-acid-residue protei

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6

Giorda, R., and H. L. Ennis. "Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes." Molecular and Cellular Biology 7, no. 6 (1987): 2097–103. http://dx.doi.org/10.1128/mcb.7.6.2097-2103.1987.

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A previously isolated cDNA clone, pLK229, that is specific for mRNA developmentally expressed during Dictyostelium discoideum spore germination and multicellular development, was used to screen two genomic libraries. Two genomic sequences homologous to pLK229 were isolated and sequenced. Genomic clone p229 is identical to the cDNA clone pLK229 and codes for a polypeptide of 381 amino acids. This polypeptide is composed of five tandem repeats of the same 76-amino-acid sequence. Clone lambda 229 codes for a protein of 229 amino acids, containing three tandem repeats of the identical 76-amino-aci

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7

Giorda, R., and H. L. Ennis. "Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes." Molecular and Cellular Biology 7, no. 6 (1987): 2097–103. http://dx.doi.org/10.1128/mcb.7.6.2097.

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A previously isolated cDNA clone, pLK229, that is specific for mRNA developmentally expressed during Dictyostelium discoideum spore germination and multicellular development, was used to screen two genomic libraries. Two genomic sequences homologous to pLK229 were isolated and sequenced. Genomic clone p229 is identical to the cDNA clone pLK229 and codes for a polypeptide of 381 amino acids. This polypeptide is composed of five tandem repeats of the same 76-amino-acid sequence. Clone lambda 229 codes for a protein of 229 amino acids, containing three tandem repeats of the identical 76-amino-aci

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8

Wypijewski, K., T. Malinowski, W. Musiał, and J. Augustyniak. "Nucleotide sequence of the coat protein gene of the Skierniewice isolate of plum pox virus (PPV)." Acta Biochimica Polonica 41, no. 1 (1994): 87–95. http://dx.doi.org/10.18388/abp.1994_4778.

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The coat protein (CP) gene of the Skierniewice isolate of plum pox virus (PPV-S) has been amplified using the reverse transcription--polymerase chain reaction (RT-PCR), cloned and sequenced. The nucleotide sequence of the gene and the deduced amino-acid sequence of PPV-S CP were compared with those of other PPV strains. The nucleotide sequence showed very high homology to most of the published sequences. The motif: Asp-Ala-Gly (DAG), important for the aphid transmissibility, was present in the amino-acid sequence. Our isolate did not react in ELISA with monoclonal antibodies MAb06 supposed to

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9

Chen, Junhong, Wei Dai, Hang Wang, Weiqiang Lei, Guangyuan Fang, and Dingzhen Dai. "Cloning and Expression of Pigeon-Derived Escherichia coli Type 1 Pilus Clusters and Analysis of Amino Acid Sequence Characteristics of Functional Proteins." Genes 15, no. 10 (2024): 1253. http://dx.doi.org/10.3390/genes15101253.

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Background: Type 1 pili, as an important virulence factor of E. coli, has certain homology between APEC and UPEC, but the homology degree is not clear enough. Objectives: This study aims to compare the homology between them. Methods: The recombinant bacteria were constructed by homologous recombination. The pili were observed by TEM, and the hemagglutination characteristics were determined by MHSA. The complete gene sequence was determined by sequencing, and the amino acid sequences of the functional proteins of type 1 pili of APEC and UPEC were compared. Results: TEM showed that they could ex

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10

Gotschlich, E. C., M. Seiff, and M. S. Blake. "The DNA sequence of the structural gene of gonococcal protein III and the flanking region containing a repetitive sequence. Homology of protein III with enterobacterial OmpA proteins." Journal of Experimental Medicine 165, no. 2 (1987): 471–82. http://dx.doi.org/10.1084/jem.165.2.471.

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The insert of a lambda gt11 clone expressing gonococcal protein III was sequenced. The deduced amino acid sequence showed a coding frame of 236 amino acids with a typical 22-amino-acid signal peptide, followed by the known NH2-terminal sequence of PIII. The mature protein has a molecular weight of 23,298. It was found that PIII had extensive and very striking homology to the carboxy-terminal portion of enterobacterial OmpA proteins. The homology encompasses the OmpA domain that is believed to be located in the periplasmic space. If the disposition of PIII across the OM is analogous, then the s

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11

Zhou, Yamei, Shencong Lv, Hao Yan, et al. "Complete Genome Sequence Analysis of a Dengue Case Co-Infected with Type I and Type II Imported into Jiaxing in 2023." Microbiology Research 15, no. 4 (2024): 2673–86. https://doi.org/10.3390/microbiolres15040177.

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In 2023, one case of coinfection with type I and type II dengue virus of imported origin was first reported in Jiaxing city. Therefore, we analysed the results of the molecular tracing analysis of this case. We collected serum samples for whole-genome amplification and sequencing and further analysed the whole-genome sequence for homology analysis, evolutionary tree analysis, and protein amino acid mutation site analysis. The results revealed that the JX202301 DENV-1 sequence had the highest homology with the epidemic strains in Guangdong (PP563909, PP563875, PP563840, and PP563879) in 2023, w

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12

Oldberg, Å., P. Antonsson, and D. Heinegård. "The partial amino acid sequence of bovine cartilage proteoglycan, deduced from a cDNA clone, contains numerous Ser-Gly sequences arranged in homologous repeats." Biochemical Journal 243, no. 1 (1987): 255–59. http://dx.doi.org/10.1042/bj2430255.

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We have determined the sequence of a partial cDNA clone encoding the C-terminal region of bovine cartilage aggregating proteoglycan core protein. The deduced amino acid sequence contains a cysteine-rich region which is homologous with chicken hepatic lectin. This lectin-homologous region has previously been identified in rat and chicken cartilage proteoglycan. The bovine sequence presented here is highly homologous with the rat and chicken amino acid sequences in this apparently globular region. A region containing clusters of Ser-Gly sequences is located N-terminal to the lectin homology doma

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13

Bradac, J. A., C. E. Gruber, S. Forry-Schaudies, and S. H. Hughes. "Isolation and characterization of related cDNA clones encoding skeletal muscle beta-tropomyosin and a low-molecular-weight nonmuscle tropomyosin isoform." Molecular and Cellular Biology 9, no. 1 (1989): 185–92. http://dx.doi.org/10.1128/mcb.9.1.185-192.1989.

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We have isolated and characterized cDNA clones from chicken cDNA libraries derived from skeletal muscle, body wall, and cultured fibroblasts. A clone isolated from a skeletal muscle cDNA library contains the complete protein-coding sequence of the 284-amino-acid skeletal muscle beta-tropomyosin together with 72 bases of 5' untranslated sequence and nearly the entire 3' untranslated region (about 660 bases), lacking only the last 4 bases and the poly(A) tail. A second clone, isolated from the fibroblast cDNA library, contains the complete protein-coding sequence of a 248-amino-acid fibroblast t

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14

Bradac, J. A., C. E. Gruber, S. Forry-Schaudies, and S. H. Hughes. "Isolation and characterization of related cDNA clones encoding skeletal muscle beta-tropomyosin and a low-molecular-weight nonmuscle tropomyosin isoform." Molecular and Cellular Biology 9, no. 1 (1989): 185–92. http://dx.doi.org/10.1128/mcb.9.1.185.

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We have isolated and characterized cDNA clones from chicken cDNA libraries derived from skeletal muscle, body wall, and cultured fibroblasts. A clone isolated from a skeletal muscle cDNA library contains the complete protein-coding sequence of the 284-amino-acid skeletal muscle beta-tropomyosin together with 72 bases of 5' untranslated sequence and nearly the entire 3' untranslated region (about 660 bases), lacking only the last 4 bases and the poly(A) tail. A second clone, isolated from the fibroblast cDNA library, contains the complete protein-coding sequence of a 248-amino-acid fibroblast t

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15

Shil, Pratip, Niraj Dudani, and Pandit B. Vidyasagar. "ISHAN: Sequence Homology Analysis Package." In Silico Biology: Journal of Biological Systems Modeling and Multi-Scale Simulation 6, no. 5 (2006): 373–77. https://doi.org/10.3233/isb-00250.

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Sequence based homology studies play an important role in evolutionary tracing and classification of proteins. Various methods are available to analyze biological sequence information. However, with the advent of proteomics era, there is a growing demand for analysis of huge amount of biological sequence information, and it has become necessary to have programs that would provide speedy analysis. ISHAN has been developed as a homology analysis package, built on various sequence analysis tools viz FASTA, ALIGN, CLUSTALW, PHYLIP and CODONW (for DNA sequences). This JAVA application offers the us

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16

Li, Bing, Yan Hong Wang, Ju Mei Wang, and Wei De Shen. "Full Length cDNA Cloning and Expression Characteristics of Ace Gene from Wild Silkworm, Bombyx mandarina." Advanced Materials Research 175-176 (January 2011): 51–55. http://dx.doi.org/10.4028/www.scientific.net/amr.175-176.51.

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Acetylcholinesterase (AChE), which contains two subfamilies, ace1 and ace2 in insects, was identified to be the target of organophosphorous and carbamate insecticides. To research the sequences and tissues expressions of two aces, full length cDNAs encoding two ace genes were cloned, designated as Bmm-ace1 and Bmm-ace2 from larvae of the Bombyx mandarina. The amino acid sequence of Bmm-ace1 shared 99.71 % homology with its homolog, Bm-ace1, in silkworm, Bombyx mori, with two mutations (G664S and S307P), and the amino acid sequence of Bmm-ace2 shared 99.37 % homology with Bm-ace2, in B. mori ,

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17

Marcus, Frank, Brigitte Gontero, Peter B. Harrsch, and Judith Rittenhouse. "Amino acid sequence homology among fructose-1,6-bisphosphatases." Biochemical and Biophysical Research Communications 135, no. 2 (1986): 374–81. http://dx.doi.org/10.1016/0006-291x(86)90005-7.

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18

Olson, Daniel, Thomas Colligan, Daphne Demekas, Jack W. Roddy, Ken Youens-Clark, and Travis J. Wheeler. "NEAR: neural embeddings for amino acid relationships." Bioinformatics 41, Supplement_1 (2025): i449—i457. https://doi.org/10.1093/bioinformatics/btaf198.

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Abstract Summary Protein language models (PLMs) have recently demonstrated potential to supplant classical protein database search methods based on sequence alignment, but are slower than common alignment-based tools and appear to be prone to a high rate of false labeling. Here, we present Neural Embeddings for Amino acid Relationships (NEAR), a method based on neural representation learning that is designed to improve both speed and accuracy of search for likely homologs in a large protein sequence database. NEAR’s ResNet embedding model is trained using contrastive learning guided by trusted

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19

Choi, Sunju, Shinya Ohta, and Eiichi Ohtsubo. "A Novel IS Element, IS621, of the IS110/IS492 Family Transposes to a Specific Site in Repetitive Extragenic Palindromic Sequences in Escherichia coli." Journal of Bacteriology 185, no. 16 (2003): 4891–900. http://dx.doi.org/10.1128/jb.185.16.4891-4900.2003.

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ABSTRACT An Escherichia coli strain, ECOR28, was found to have insertions of an identical sequence (1,279 bp in length) at 10 loci in its genome. This insertion sequence (named IS621) has one large open reading frame encoding a putative protein that is 326 amino acids in length. A computer-aided homology search using the DNA sequence as the query revealed that IS621 was homologous to the piv genes, encoding pilin gene invertase (PIV). A homology search using the amino acid sequence of the putative protein encoded by IS621 as the query revealed that the protein also has partial homology to tran

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20

Kim, Keonhee, Chae-Hong Park, and Soon-Jin Hwang. "Analyzing the Akinete Protein of the Harmful Freshwater Cyanobacterium, Dolichospermum circinale." Water 15, no. 15 (2023): 2746. http://dx.doi.org/10.3390/w15152746.

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Akinete is a survival structure in cyanobacteria that has overcome unfavorable environmental conditions and influences their perennial blooms in the freshwater system. However, the akinete cellular and biochemical properties are insufficiently explored. We analyzed the akinete structure, as well as akinete-specific proteins and their amino acid sequence. Akinetes of Dolichospermum circinale were produced from their vegetative cells isolated from the North Han River, Korea. The akinete protein was obtained using electrophoresis, and utilizing its amino acid sequences, its antibody-binding react

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21

Dang, Tu Thi My, Thao Thi Tran, Tien My Van, Quang Trung Le, and Bich Tran Ngoc. "First molecular report of Feline panleukopenia virus infection in diarrheic cats at Can Tho City, Vietnam." Veterinary Integrative Sciences 22, no. 2 (2023): 631–43. http://dx.doi.org/10.12982/vis.2024.043.

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Feline panleukopenia virus (FPV) belongs to the family Parvoviridae and causes an acute viral infection in cats worldwide. Information on the circulation of FPV among cats is currently limited in Vietnam. Herein, the full–length VP2 gene and molecular characteristics of FPV isolated in diarrhea cats in Can Tho City were first exhibited. Phylogenetic analysis based on seven obtained nucleotide sequences revealed that the isolated sequences were clustered into a narrow group with FPV sequences in the neighboring countries such as China, Thailand, and Japan, and distantly grouped with the vaccine

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22

Steiner, Bret, Sandra Romero-Steiner, Donna Cruce, and Robert George. "Cloning and sequencing of the hyaluronate lyase gene fromPropionibacterium acnes." Canadian Journal of Microbiology 43, no. 4 (1997): 315–21. http://dx.doi.org/10.1139/m97-044.

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The hyaluronate lyase (hyaluronidase) gene from Propionibacterium acnes was cloned and sequenced. The gene was isolated on an EcoRI-generated 3-kb piece of DNA. Expression was less in Escherichia coli than in P. acnes; in E. coli, active enzyme was only cell associated and not secreted. The gene is 2256-bp long and codes for a protein of 82 kDa. Amino terminal sequencing of the protein of the partially purified gene indicated the presence of a 32-amino-acid leader sequence. The leader sequence showed a membrane-spanning domain and all of the features usually associated with the leader for a se

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Lawler, J., and R. O. Hynes. "The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins." Journal of Cell Biology 103, no. 5 (1986): 1635–48. http://dx.doi.org/10.1083/jcb.103.5.1635.

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Thrombospondin is one of a class of adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. We have used two monoclonal antibodies to isolate cDNA clones of thrombospondin from a human endothelial cell cDNA library and have determined the complete nucleotide sequence of the coding region. Three regions of known amino acid sequence of human platelet thrombospondin confirm that the clones are authentic. Three types of repeating amino acid sequence are present in thrombospondin. The first is 57 amino acids long and shows homology with circumsporozoite protein from Plasmo

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Shoemaker, C. B., and L. D. Mitsock. "Murine erythropoietin gene: cloning, expression, and human gene homology." Molecular and Cellular Biology 6, no. 3 (1986): 849–58. http://dx.doi.org/10.1128/mcb.6.3.849-858.1986.

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The gene for murine erythropoietin (EPO) was isolated from a mouse genomic library with a human EPO cDNA probe. Nucleotide sequence analysis permitted the identification of the murine EPO coding sequence and the prediction of the encoded amino acid sequence based on sequence conservation between the mouse and human EPO genes. Both the coding DNA and the amino acid sequences were 80% conserved between the two species. Transformation of COS-1 cells with a mammalian cell expression vector containing the murine EPO coding region resulted in secretion of murine EPO with biological activity on both

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Shoemaker, C. B., and L. D. Mitsock. "Murine erythropoietin gene: cloning, expression, and human gene homology." Molecular and Cellular Biology 6, no. 3 (1986): 849–58. http://dx.doi.org/10.1128/mcb.6.3.849.

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The gene for murine erythropoietin (EPO) was isolated from a mouse genomic library with a human EPO cDNA probe. Nucleotide sequence analysis permitted the identification of the murine EPO coding sequence and the prediction of the encoded amino acid sequence based on sequence conservation between the mouse and human EPO genes. Both the coding DNA and the amino acid sequences were 80% conserved between the two species. Transformation of COS-1 cells with a mammalian cell expression vector containing the murine EPO coding region resulted in secretion of murine EPO with biological activity on both

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26

Purwantini, Endang, and Lacy Daniels. "Molecular Analysis of the Gene Encoding F420-Dependent Glucose-6-Phosphate Dehydrogenase fromMycobacterium smegmatis." Journal of Bacteriology 180, no. 8 (1998): 2212–19. http://dx.doi.org/10.1128/jb.180.8.2212-2219.1998.

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ABSTRACT The gene fgd, which codes for F420-dependent glucose-6-phosphate dehydrogenase (FGD), was cloned from Mycobacterium smegmatis, and its sequence was determined and analyzed. A homolog of FGD which has a very high similarity to the M. smegmatis FGD-derived amino acid sequence was identified in Mycobacterium tuberculosis. FGD showed significant homology with F420-dependentN 5,N 10-methylene-tetrahydromethanopterin reductase (MER) from methanogenic archaea and with several hypothetical proteins from M. tuberculosis andArchaeoglobus fulgidus, but FGD showed no significant homology with NAD

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Tsuge, Kenji, Takanori Akiyama, and Makoto Shoda. "Cloning, Sequencing, and Characterization of the Iturin A Operon." Journal of Bacteriology 183, no. 21 (2001): 6265–73. http://dx.doi.org/10.1128/jb.183.21.6265-6273.2001.

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ABSTRACT Bacillus subtilis RB14 is a producer of the antifungal lipopeptide iturin A. Using a transposon, we identified and cloned the iturin A synthetase operon of RB14, and the sequence of this operon was also determined. The iturin A operon spans a region that is more than 38 kb long and is composed of four open reading frames, ituD, ituA, ituB, and ituC. The ituD gene encodes a putative malonyl coenzyme A transacylase, whose disruption results in a specific deficiency in iturin A production. The second gene, ituA, encodes a 449-kDa protein that has three functional modules homologous to fa

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Kirana, Maha, Rahaju Ernawati, Jola Rahmahani, and Fedik Abdul Rantam. "Characterization of Gene Coding Fusion Protein of Newcastle Disease Virus Infected in Native Chicken in Surabaya." Jurnal Medik Veteriner 5, no. 1 (2022): 103–8. http://dx.doi.org/10.20473/jmv.vol5.iss1.2022.103-108.

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This study aimed to discover the homology of nucleotide sequence, homology percentage, and those relations phylogenetic of protein Fusion (F) gene coding of Newcastle disease in domestic chicken (Gallus gallus domesticus) in Surabaya using some comparison isolate from GenBank. Samples were scoured of digestive organs from native chicken, that was collected from a traditional market in Wonokromo, Surabaya. Samples were tested using RT-PCR with primer forward and reverse with target 976bp, a positive sample which is continued with sequencing then homology and nucleotide analysis which is done an

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Szopa, J. "Expression analysis of a Cucurbita cDNA encoding endonuclease." Acta Biochimica Polonica 42, no. 2 (1995): 183–89. http://dx.doi.org/10.18388/abp.1995_4643.

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The nuclear matrices of plant cell nuclei display intrinsic nuclease activity which consists in nicking supercoiled DNA. A cDNA encoding a 32 kDa endonuclease has been cloned and sequenced. The nucleotide and deduced amino-acid sequences show high homology to known 14-3-3 protein sequences from other sources. The amino-acid sequence shows agreement with consensus sequences for potential phosphorylation by protein kinase A and C and for calcium, lipid and membrane-binding sites. The nucleotide-binding site is also present within the conserved part of the sequence. By Northern blot analysis, the

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Wu, C. T., M. Budding, M. S. Griffin, and J. M. Croop. "Isolation and characterization of Drosophila multidrug resistance gene homologs." Molecular and Cellular Biology 11, no. 8 (1991): 3940–48. http://dx.doi.org/10.1128/mcb.11.8.3940-3948.1991.

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Mammalian multidrug-resistant cell lines, selected for resistance to a single cytotoxic agent, display cross-resistance to a broad spectrum of structurally and functionally unrelated compounds. These cell lines overproduce a membrane protein, the P-glycoprotein, which is encoded by a member(s) of a multigene family, termed mdr or pgp. The amino acid sequence of the P-glycoprotein predicts an energy-dependent transport protein with homology to a large superfamily of proteins which transport a wide variety of substances. This report describes the isolation and characterization of two Drosophila

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Wu, C. T., M. Budding, M. S. Griffin, and J. M. Croop. "Isolation and characterization of Drosophila multidrug resistance gene homologs." Molecular and Cellular Biology 11, no. 8 (1991): 3940–48. http://dx.doi.org/10.1128/mcb.11.8.3940.

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Mammalian multidrug-resistant cell lines, selected for resistance to a single cytotoxic agent, display cross-resistance to a broad spectrum of structurally and functionally unrelated compounds. These cell lines overproduce a membrane protein, the P-glycoprotein, which is encoded by a member(s) of a multigene family, termed mdr or pgp. The amino acid sequence of the P-glycoprotein predicts an energy-dependent transport protein with homology to a large superfamily of proteins which transport a wide variety of substances. This report describes the isolation and characterization of two Drosophila

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32

Kusano, A., M. Iwama, A. Sanda, et al. "Primary structure of porcine spleen ribonuclease: sequence homology." Acta Biochimica Polonica 44, no. 4 (1997): 689–99. http://dx.doi.org/10.18388/abp.1997_4371.

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The primary structure of porcine spleen RNase (RNase Psp1) was investigated as a mean of assessing the structure-function relationship of base non-specific ribonucleases of animal origin. N-terminal analysis of RNase Psp1 yielded three N-terminal sequences. These peptides were separated by gel-filtration on Superdex 75HR, after reduction and S-carboxymethylation of RNase Psp1. Determination of the amino-acid sequence of these peptides indicated that the RNase Psp1 preparation consisted of three peptides having 20 (RCM RNase Psp1 pep1), 15 (RCM RNase Psp1 pep2), and 164 (RCM RNase Psp1 pro) ami

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Nelapati, Anand Kumar, and JagadeeshBabu PonnanEttiyappan. "Computational Analysis of Therapeutic Enzyme Uricase from Different Source Organisms." Current Proteomics 17, no. 1 (2020): 59–77. http://dx.doi.org/10.2174/1570164616666190617165107.

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Background:Hyperuricemia and gout are the conditions, which is a response of accumulation of uric acid in the blood and urine. Uric acid is the product of purine metabolic pathway in humans. Uricase is a therapeutic enzyme that can enzymatically reduces the concentration of uric acid in serum and urine into more a soluble allantoin. Uricases are widely available in several sources like bacteria, fungi, yeast, plants and animals.Objective:The present study is aimed at elucidating the structure and physiochemical properties of uricase by insilico analysis.Methods:A total number of sixty amino ac

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Holm, E., K. Sletten та G. Husby. "Structural studies of a carbohydrate-containing immunoglobulin-λ-light-chain amyloid-fibril protein (AL) of variable subgroup III". Biochemical Journal 239, № 3 (1986): 545–51. http://dx.doi.org/10.1042/bj2390545.

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The amino acid sequence of the variable region of a carbohydrate-containing amyloid-fibril protein MOL of immunoglobulin-light-chain type (AL) was elucidated. The sequence determination involved cleaving the protein with CNBr, BNPS-skatole, thermolysin and trypsin. The sequenced protein consisted of about 130 amino acid residues; however, gel-filtration and N-terminal analysis studies revealed AL proteins ranging in Mr from about 10,000 to 25,000. The oligosaccharide chain was found to be bound in the hypervariable region. By sequence homology to other lambda chains the AL protein MOL was show

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Salzer, JL, WP Holmes, and DR Colman. "The amino acid sequences of the myelin-associated glycoproteins: homology to the immunoglobulin gene superfamily." Journal of Cell Biology 104, no. 4 (1987): 957–65. http://dx.doi.org/10.1083/jcb.104.4.957.

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The myelin associated glycoproteins (MAG) are integral plasma membrane proteins which are found in oligodendrocytes and Schwann cells and are believed to mediate the axonal-glial interactions of myelination. In this paper we demonstrate the existence in central nervous system myelin of two MAG polypeptides with Mrs of 67,000 and 72,000 that we have designated small MAG (S-MAG) and large MAG (L-MAG), respectively. The complete amino acid sequence of L-MAG and a partial amino acid sequence of S-MAG have been deduced from the nucleotide sequences of corresponding cDNA clones isolated from a lambd

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Wu, Mi, Zhifei Zhang, Xin Su, et al. "Biological Characteristics of Infectious Laryngotracheitis Viruses Isolated in China." Viruses 14, no. 6 (2022): 1200. http://dx.doi.org/10.3390/v14061200.

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Infectious laryngotracheitis virus (ILTV) causes severe respiratory disease in chickens and results in huge economic losses in the poultry industry worldwide. To correlate the genomic difference with the replication and pathogenicity, phenotypes of three ILTVs isolated from chickens in China from 2016 to 2018 were sequenced by high-throughput sequencing. Based on the entire genome, the isolates GD2018 and SH2017 shared 99.9% nucleotide homology, while the isolate SH2016 shared 99.7% nucleotide homology with GD2018 and SH2017, respectively. Each virus genome contained 82 ORFs encoding 77 kinds

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Suzuki, T., T. Takagi, and S. Ohta. "Primary structure of a dimeric haemoglobin from the deep-sea cold-seep clam Calyptogena soyoae." Biochemical Journal 260, no. 1 (1989): 177–82. http://dx.doi.org/10.1042/bj2600177.

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The heterodont clam Calyptogena soyoae, living in the cold-seep area of the upper bathyal depth of Sagami Bay, Japan, has two homodimeric haemoglobins (Hb I and Hb II) in erythrocytes. The complete amino acid sequence of 136 residues of C. soyoae Hb II was determined. The sequence showed low homology with any other globins (at most 20% identity) and lacked the N-terminal extension of seven to nine amino acid residues characteristic of all the molluscan haemoglobins sequenced hitherto. Although the subunit assembly of molluscan haemoglobin is known to be ‘back-to-front’ relative to vertebrate h

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Hellman, U., G. Eggertsen, A. Engström, and J. Sjöquist. "Amino acid sequence of the trypsin-generated C3d fragment from human complement factor C3." Biochemical Journal 230, no. 2 (1985): 353–61. http://dx.doi.org/10.1042/bj2300353.

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Human C3d (try-C3d), prepared from trypsin-digested C3, was fragmented by cleavage with CNBr. Eight peptides were defined and separated by h.p.l.c. on reversed-phase columns. By automatic Edman degradation the complete sequences of five peptides and partial sequences of three peptides were determined. To obtain overlapping peptides the latter three fragments were digested with trypsin, chymotrypsin or Staphylococcus aureus V8 proteinase, after which the fragments were separated on reversed-phase columns. Two of the CNBr-cleavage peptides were completely sequenced, and 70% of the sequence of th

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Stevenson, M. A., and S. K. Calderwood. "Members of the 70-kilodalton heat shock protein family contain a highly conserved calmodulin-binding domain." Molecular and Cellular Biology 10, no. 3 (1990): 1234–38. http://dx.doi.org/10.1128/mcb.10.3.1234-1238.1990.

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The 70-kilodalton heat shock protein (hsp70) family members appear to be essential components in a number cellular protein-protein interactions. We report here on the characterization of a new functional region in hsp70, a calmodulin-binding site. We have identified a 21-amino-acid sequence within the hsp70 protein that contains a calmodulin-binding domain. The peptide formed a potential amphipathic alpha helix and bound calmodulin with high affinity. Comparison of amino acid homology of this calmodulin-binding sequence with analogous hsp70 sequences from other species showed a high degree of

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Stevenson, M. A., and S. K. Calderwood. "Members of the 70-kilodalton heat shock protein family contain a highly conserved calmodulin-binding domain." Molecular and Cellular Biology 10, no. 3 (1990): 1234–38. http://dx.doi.org/10.1128/mcb.10.3.1234.

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The 70-kilodalton heat shock protein (hsp70) family members appear to be essential components in a number cellular protein-protein interactions. We report here on the characterization of a new functional region in hsp70, a calmodulin-binding site. We have identified a 21-amino-acid sequence within the hsp70 protein that contains a calmodulin-binding domain. The peptide formed a potential amphipathic alpha helix and bound calmodulin with high affinity. Comparison of amino acid homology of this calmodulin-binding sequence with analogous hsp70 sequences from other species showed a high degree of

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Foley, R. C., and K. J. Beh. "Isolation and sequence of sheep Ig H and L chain cDNA." Journal of Immunology 142, no. 2 (1989): 708–11. http://dx.doi.org/10.4049/jimmunol.142.2.708.

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Abstract Sheep lymphocyte poly(A+) RNA was used as a template for the enzymatic synthesis of cDNA before cloning into the expression vector lambda gt11. Screening of the cDNA library with mAb probes resulted in the isolation of two recombinant phages containing Ig coding sequences of 704 bp and 925 bp. These were inserted into the EcoRI site of pUC18 and named pSLC (sheep Ig L chain) and pSHC (sheep Ig H chain). The insert in pSLC revealed sequence homology by using GenBank to lambda L chain and pSHC revealed sequence homology to IgG sequences from various species. The L chain cDNA contained t

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Tate, C. G., та M. J. A. Tanner. "Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins α and δ". Biochemical Journal 254, № 3 (1988): 743–50. http://dx.doi.org/10.1042/bj2540743.

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We have isolated almost full-length cDNA clones corresponding to human erythrocyte membrane sialoglycoproteins alpha (glycophorin A) and delta (glycophorin B). The predicted amino acid sequence of delta differs at two amino acid residues from the sequence determined by peptide sequencing. The sialoglycoprotein delta clone we have isolated contains an interrupting sequence within the region that gives rise to the cleaved N-terminal leader sequence for the protein and represents a product that is unlikely to be inserted into the erythrocyte membrane. Comparison of the cDNA sequences of alpha and

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Qaisar, U., M. Irfan, A. Meqbool, et al. "Identification, sequencing and characterization of a stress induced homologue of fructose bisphosphate aldolase from cotton." Canadian Journal of Plant Science 90, no. 1 (2010): 41–48. http://dx.doi.org/10.4141/cjps08056.

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A drought-induced homologue of fructose-1, 6 bisphosphate aldolase full length cDNA (GaAldp) was isolated and sequenced by gene homology and rapid amplification of cDNA ends (RACE) from cotton variety FDH-786 (Gossypium arboretum). Quantitative polymerase chain reaction (PCR) analysis showed that drought stress, salinity and exogenous treatment with abscisic acid (ABA) enhanced the accumulation of GaAldp transcripts in the leaf and stem tissues of the plant. An alignment of the 1413 bp cDNA and 1937 bp genomic DNA sequences revealed that GaAldp has an open reading frame (ORF) encoding 394 amin

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Collyear, K., S. I. Girgis, G. Saunders, I. MacIntyre, and G. Holt. "Predicted structure of the bovine calcitonin gene-related peptide and the carboxy-terminal flanking peptide of bovine calcitonin precursor." Journal of Molecular Endocrinology 6, no. 2 (1991): 147–52. http://dx.doi.org/10.1677/jme.0.0060147.

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ABSTRACT We have isolated from a bovine genomic library a clone which contains the calcitonin (CT) and CT gene-related peptide (CGRP) sequences, using probes representing the human CT and CGRP sequences. Sequence analysis has identified the nucleotide sequence coding for bovine CT, its C-terminal flanking peptide and bovine CGRP. The deduced amino acid sequence of bovine CGRP revealed a significant homology with other CGRPs so far reported. It differs by only one amino acid from rat CGRPα and porcine CGRP, and by three and four amino acids from human CGRPβ and α respectively. Bovine CT has, ho

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Russell, Bonnie L., Nishal Parbhoo, and Samantha Gildenhuys. "Analysis of Conserved, Computationally Predicted Epitope Regions for VP5 and VP7 Across three Orbiviruses." Bioinformatics and Biology Insights 12 (January 1, 2018): 117793221875534. http://dx.doi.org/10.1177/1177932218755348.

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Orbiviruses are double-stranded RNA viruses that have profound economic and veterinary significance, 3 of the most important being African horse sickness virus (AHSV), bluetongue virus (BTV), and epizootic hemorrhagic disease virus (EHDV). Currently, vaccination and vector control are used as preventative measures; however, there are several problems with the current vaccines. Comparing viral amino acid sequences, we obtained an AHSV-BTV-EHDV consensus sequence for VP5 (viral protein 5) and for VP7 (viral protein 7) and generated homology models for these proteins. The structures and sequences

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Winkles, J. A., T. D. Sargent, D. A. Parry, E. Jonas, and I. B. Dawid. "Developmentally regulated cytokeratin gene in Xenopus laevis." Molecular and Cellular Biology 5, no. 10 (1985): 2575–81. http://dx.doi.org/10.1128/mcb.5.10.2575-2581.1985.

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We have determined the sequence of cloned cDNAs derived from a 1,665-nucleotide mRNA which transiently accumulates during Xenopus laevis embryogenesis. Computer analysis of the deduced amino acid sequence revealed that this mRNA encodes a 47-kilodalton type I intermediate filament subunit, i.e., a cytokeratin. As is common to all intermediate filament subunits so far examined, the predicted polypeptide, named XK70, contains N- and C-terminal domains flanking a central alpha-helical rod domain. The overall amino acid homology between XK70 and a human 50-kilodalton type I keratin is 47%; homolog

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47

Winkles, J. A., T. D. Sargent, D. A. Parry, E. Jonas, and I. B. Dawid. "Developmentally regulated cytokeratin gene in Xenopus laevis." Molecular and Cellular Biology 5, no. 10 (1985): 2575–81. http://dx.doi.org/10.1128/mcb.5.10.2575.

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We have determined the sequence of cloned cDNAs derived from a 1,665-nucleotide mRNA which transiently accumulates during Xenopus laevis embryogenesis. Computer analysis of the deduced amino acid sequence revealed that this mRNA encodes a 47-kilodalton type I intermediate filament subunit, i.e., a cytokeratin. As is common to all intermediate filament subunits so far examined, the predicted polypeptide, named XK70, contains N- and C-terminal domains flanking a central alpha-helical rod domain. The overall amino acid homology between XK70 and a human 50-kilodalton type I keratin is 47%; homolog

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48

Kukuła, Maciej, Beata Hanus-Lorenz, Ewa Bok, Jacek Leluk та Aleksander F. Sikorski. "Proteins with Spectrin Motifs Which Do Not Belong to the Spectrin-α-Actinin- Dystrophin Family". Zeitschrift für Naturforschung C 59, № 7-8 (2004): 565–71. http://dx.doi.org/10.1515/znc-2004-7-821.

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AbstractUsing several consensus sequences for the 106 amino acid residue α-spectrin repeat segment as probes we searched animal sequence databases using the BLAST program in order to find proteins revealing limited, but significant similarity to spectrin. Among many spectrins and proteins from the spectrin-α-actinin-dystrophin family as well as sequences showing a rather high degree of similarity in very short stretches, we found seven homologous animal sequences of low overall similarity to spectrin but showing the presence of one or more spectrin-repeat motifs. The homology relationship of t

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Khan, Ashraf A., Eungbin Kim, and Carl E. Cerniglia. "Molecular Cloning, Nucleotide Sequence, and Expression in Escherichia coli of a Hemolytic Toxin (Aerolysin) Gene from Aeromonas trota." Applied and Environmental Microbiology 64, no. 7 (1998): 2473–78. http://dx.doi.org/10.1128/aem.64.7.2473-2478.1998.

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ABSTRACT Aeromonas trota AK2, which was derived from ATCC 49659 and produces the extracellular pore-forming hemolytic toxin aerolysin, was mutagenized with the transposon mini-Tn5Km1 to generate a hemolysin-deficient mutant, designated strain AK253. Southern blotting data indicated that an 8.7-kb NotI fragment of the genomic DNA of strain AK253 contained the kanamycin resistance gene of mini-Tn5Km1. The 8.7-kb NotI DNA fragment was cloned into the vector pGEM5Zf(−) by selecting for kanamycin resistance, and the resultant clone, pAK71, showed aerolysin activity in Escherichia coli JM109. The nu

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Jenne, D., C. Rey, D. Masson, et al. "cDNA cloning of granzyme C, a granule-associated serine protease of cytolytic T lymphocytes." Journal of Immunology 140, no. 1 (1988): 318–23. http://dx.doi.org/10.4049/jimmunol.140.1.318.

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Abstract A cDNA clone corresponding to the complete amino acid sequence of a putative protease CCP2 of murine cytotoxic T lymphocytes was isolated and sequenced. The clone encodes a 248-residue long serine esterase. The deduced N-terminal amino acid sequence is identical over 40 residues to that of granzyme C, a protease of unknown function present in granules of cytotoxic lymphocytes. Analysis of the sequence of granzyme C/CCP2 reveals high homology to other granzyme proteases, i.e. granzyme A (40%) and granzyme B (67%) and to rat mast cell protease II (46%). The amino acids lining the specif

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