Academic literature on the topic 'Ammonia assimilation'

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Journal articles on the topic "Ammonia assimilation"

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Kusnan, M. B., K. Klug, and H. P. Fock. "Ammonia Assimilation by Aspergillus nidulans: [15N]Ammonia Study." Microbiology 135, no. 4 (April 1, 1989): 729–38. http://dx.doi.org/10.1099/00221287-135-4-729.

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Magasanik, Boris. "Ammonia Assimilation by Saccharomyces cerevisiae." Eukaryotic Cell 2, no. 5 (October 2003): 827–29. http://dx.doi.org/10.1128/ec.2.5.827-829.2003.

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Mikeš, V., H. Chválová, and L. Mátlová. "Assimilation of ammonia inParacoccus denitrificans." Folia Microbiologica 36, no. 1 (February 1991): 35–41. http://dx.doi.org/10.1007/bf02935820.

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Vargas, Aracelis, and William R. Strohl. "Ammonia assimilation and metabolism byBeggiatoa alba." Archives of Microbiology 142, no. 3 (August 1985): 275–78. http://dx.doi.org/10.1007/bf00693403.

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Bessagnet, Bertrand, Laurent Menut, Florian Couvidat, Frédérik Meleux, Guillaume Siour, and Sylvain Mailler. "What Can We Expect from Data Assimilation for Air Quality Forecast? Part II: Analysis with a Semi-Real Case." Journal of Atmospheric and Oceanic Technology 36, no. 7 (July 2019): 1433–48. http://dx.doi.org/10.1175/jtech-d-18-0117.1.

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AbstractAssimilation of observational data from ground stations and satellites has been identified as a technique to improve air quality model results. This study is an evaluation of the maximum benefit expected from data assimilation in chemical transport models. Various tests are performed under real meteorological conditions; the injection of various subsets of “simulated observational data” at the initial state of a forecasting period is analyzed in terms of benefit on selected criteria. This observation dataset is generated by a simulation with perturbed input data. Several criteria are defined to analyze the simulations leading to the definition of a “tipping time” to compare the behavior of simulations. Assimilating three-dimensional data instead of ground observations clearly adds value to the forecast. For the studied period and considering the expected best favorable data assimilation experiment, the maximum benefit is higher for particulate matter (PM) with tipping times exceeding 80 h; for ozone (O3) the gain is on average around 30 h. Assimilating O3 concentrations with a delta calculated on the first level and propagated over the vertical direction provides better results on O3 mean concentrations when compared with the expected best experiment corresponding to the injection of the O3 “observations” 3D dataset, but for maximum O3 concentrations the opposite behavior is observed. If data assimilation of secondary pollutant concentrations provides an improvement, assimilation of primary pollutant emissions can have beneficial impacts when compared with an assimilation of concentrations, after several days on secondary pollutants like O3 or nitrate concentrations and more quickly for the emitted primary pollutants. An assimilation of ammonia concentrations has slightly better performances on nitrate, ammonium, and PM concentrations relative to the assimilation of nitrogen or sulfur dioxides.
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Lee, R., J. Childress, and N. Desaulniers. "The effects of exposure to ammonia on ammonia and taurine pools of the symbiotic clam." Journal of Experimental Biology 200, no. 21 (November 1, 1997): 2797–805. http://dx.doi.org/10.1242/jeb.200.21.2797.

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The nutrition of the gutless clam Solemyareidi is supported by the activity of intracellular chemoautotrophic bacteria housed in its gill filaments. Ammonia (the sum of NH3 and NH4+) is utilized as a nitrogen source by the association and is abundant in the clam's environment. In the present study, clams were exposed to 0.01­1.3mmoll-1 ammonia for 22­23h in the presence of thiosulfate as a sulfur substrate. Ammonia exposure increased the ammonia concentration in the tissue pools of the gill, foot and visceral mass from 0.5 to 2µmolg-1wetmass, without added ammonia, to as much as 12µmolg-1wetmass in the presence of 0.7 and 1.3mmoll-1 external ammonia. Gill tissue ammonia concentrations were consistently higher than those in the foot and visceral mass. The elevation of tissue ammonia concentration compared with the medium may be due in part to an ammonia trapping mechanism resulting from a lower intracellular pH compared with sea water and greater permeability to NH3 compared with NH4+. Rates of ammonia incorporation into organic matter (assimilation) were determined using 15N as a tracer. 15N-labeled ammonia assimilation was higher in gill than in foot and increased as a function of 15N-labeled ammonia concentration in the medium. The size of the free amino acid (FAA) pool in the gill also increased as a function of ammonia concentration in the medium. This entire increase was accounted for by a single amino acid, taurine, which was the predominant FAA in both gill and foot tissue. Aspartate, glutamate, arginine and alanine were also abundant but their levels were not influenced by external ammonia concentration. Ammonia assimilation appeared to occur at rates sufficient to account for the observed increase in taurine level. These findings suggest that taurine is a major product of ammonia assimilation.
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Pengpeng, Wang, and Zhiliang Tan. "Ammonia Assimilation in Rumen Bacteria: A Review." Animal Biotechnology 24, no. 2 (April 2013): 107–28. http://dx.doi.org/10.1080/10495398.2012.756402.

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Lacerda, V., A. Marsden, and W. M. Ledingham. "Ammonia assimilation inS. cerevisiae under chemostatic growth." Applied Biochemistry and Biotechnology 32, no. 1-3 (January 1992): 15–21. http://dx.doi.org/10.1007/bf02922145.

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Lee, R. W., J. J. Robinson, and C. M. Cavanaugh. "Pathways of inorganic nitrogen assimilation in chemoautotrophic bacteria-marine invertebrate symbioses: expression of host and symbiont glutamine synthetase." Journal of Experimental Biology 202, no. 3 (February 1, 1999): 289–300. http://dx.doi.org/10.1242/jeb.202.3.289.

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Symbioses between chemoautotrophic bacteria and marine invertebrates living at deep-sea hydrothermal vents and other sulfide-rich environments function autotrophically by oxidizing hydrogen sulfide as an energy source and fixing carbon dioxide into organic compounds. For chemoautotrophy to support growth, these symbioses must be capable of inorganic nitrogen assimilation, a process that is not well understood in these or other aquatic symbioses. Pathways of inorganic nitrogen assimilation were investigated in several of these symbioses: the vent tubeworms Riftia pachyptila and Tevnia jerichonana, the vent bivalves Calyptogena magnifica and Bathymodiolus thermophilus, and the coastal bivalve Solemya velum. Nitrate reductase activity was detected in R. pachyptila, T. jerichonana and B. thermophilus, but not in C. magnifica and S. velum. This is evidence for nitrate utilization, either assimilation or respiration, by some vent species and is consistent with the high levels of nitrate availability at vents. The ammonia assimilation enzymes glutamine synthetase (GS) and glutamate dehydrogenase (GDH) were detected in all symbioses tested, indicating that ammonia resulting from nitrate reduction or from environmental uptake can be incorporated into amino acids. A complicating factor is that GS and GDH are potentially of both host and symbiont origin, making it unclear which partner is involved in assimilation. GS, which is considered to be the primary ammonia-assimilating enzyme of autotrophs, was investigated further. Using a combination of molecular and biochemical approaches, host and symbiont GS were distinguished in the intact association. On the basis of Southern hybridizations, immunoreactivity, subunit size and thermal stability, symbiont GS was found to be a prokaryote GS. Host GS was distinct from prokaryote GS. The activities of host and symbiont GS were separated by anion-exchange chromatography and quantified. Virtually all activity in symbiont-containing tissue was due to symbiont GS in R. pachyptila, C. magnifica and B. thermophilus. In contrast, no symbiont GS activity was detected in the gill of S. velum, the predominant activity in this species appearing to be host GS. These findings suggest that ammonia is primarily assimilated by the symbionts in vent symbioses, whereas in S. velum ammonia is first assimilated by the host. The relationship between varying patterns of GS expression and host-symbiont nutritional exchange is discussed.
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Michel-Reydellet, Nathalie, and P. Alexandre Kaminski. "Azorhizobium caulinodans PIIand GlnK Proteins Control Nitrogen Fixation and Ammonia Assimilation." Journal of Bacteriology 181, no. 8 (April 15, 1999): 2655–58. http://dx.doi.org/10.1128/jb.181.8.2655-2658.1999.

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ABSTRACT We herein report that Azorhizobium caulinodansPII and GlnK are not necessary for glutamine synthetase (GS) adenylylation whereas both proteins are required for complete GS deadenylylation. The disruption of both glnB andglnK resulted in a high level of GS adenylylation under the condition of nitrogen fixation, leading to ammonium excretion in the free-living state. PII and GlnK also controllednif gene expression because NifA activated nifHtranscription and nitrogenase activity was derepressed in glnB glnK double mutants, but not in wild-type bacteria, grown in the presence of ammonia.
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Dissertations / Theses on the topic "Ammonia assimilation"

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Lacerda, Maria Virginia Campos. "Ammonia assimilation in Saccharomyces cerevisiae under chemostatic growth." Thesis, University of St Andrews, 1991. http://hdl.handle.net/10023/14034.

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In order to investigate the effect of the elimination of GOGAT activity in S. cerevisiae, the pool sizes of ammonia, glutamate and glutamine plus the specific activities of the enzymes involved in ammonia assimilation were determined for two genetically engineered strains (AR2 and AR5) and an haploid wild type ( Sigma 1278b). AR2 and AR5 strains carry the plasmid pCYG4 which directs about 5 fold more NADPH-GDH activity than wild type cells. AR5 strain is a double mutant, which lacks GOGAT activity. The studies were carried out using a microprocessor-controlled fermenter (PCS) which has the following features: 3 Main Boards (Central Processor Board, Memory Board and Analog/Digital - ON/OFF Switch Board). 4 Auxiliary Boards (pH, Oxygen, Temperature and Biomass Interface Boards). A connection block to link the PCS with the video terminal, with sensors from the fermenter, with a control box and with other microcomputer. AR2 and AR5 showed lower values of maximum specific growth rates than the wild type, determined either by batch mode or by washout kinetics. The reduction in the growth rate for AR2 and AR5 can be related to the added metabolic loads due to the plasmid encoded genes. Under carbon limitation there were no remarkable differences between the NADPH-GDH activities of AR2 (GOGAT+) and AR5 (GOGAT-). However, the concentrations of glutamate and glutamine for AR2 were higher (from 20 to 40 %) than those of AR5. The lack of the GOGAT activity also resulted in a decrease in the biomass concentration for AR5 compared to the GOGAT+ strains. Under nitrogen limitation NADPH-GDH activities were higher and intracellular ammonia concentrations lower than under carbon limited conditions. The intracellular concentrations of glutamate and glutamine were higher for the GOGAT+ strain than for the GOGAT- one. Although the biomass level was the same for the three strains, AR5 (GOGAT") cells changed from rounded to ellipsoidal form under nitrogen limited conditions. Oscillations were present in the NADPH-GDH activities of AR2 and AR5 strains growing under carbon and nitrogen limited media. They are probably due to segregational instability of the plasmid pCYG4 in these microorganisms.
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Edwards, Nicholas John. "Nitrogen assimilation by rumen microorganisms: a study of the assimilation of ammonia by rumen bacteria in vivo and in vitro." Title page, table of contents and abstract only, 1991. http://web4.library.adelaide.edu.au/theses/09PH/09phe2657.pdf.

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Cardy, Donald Leonard Nicholas. "The molecular biology of ammonia assimilation in the obligate methanotroph Methylococcus capsulatus strain Bath." Thesis, University of Warwick, 1989. http://wrap.warwick.ac.uk/110004/.

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The structural gene (g1nA) encoding the ammonia assimilation enzyme glutamine synthetase (GS) has been cloned from the obligate methanotroph Methylococcus capsulatus (Bath). Complementation of Escherichia coli and Klebsiella pneumoniae g1nA mutants was demonstrated. In vitro and in vivo expression analysis revealed the cloned g1nA gene to encode a polypeptide with an apparent M_r of 60,000 as determined by PAGE. Expression of the M. capsulatus (Bath) g1nA gene in E. coli was found to be regulated by nitrogen levels in an Ntr+ but not an Ntr− background. This regulation was not observed when the cloned H. capsulatus (Bath) g1nA gene was under the influence of the chloramphenicol acetyl transferase gene of the vector. The nucleotide sequence of the M. capsulatus (Bath) g1nA gene and flanking sequences has also been determined. The gene comprises 1407 bp encoding a polypeptide of M_r 51,717 containing 468 amino acids. The 5' leader region contains three putative promoters. Promoters P1 and P3 resemble the canonical -10 -35 E. coli type promoter. Promoter P2 which is located between P1 and P3, resembles the NtrA dependent promoters of enteric organisms. A potential NtrC-binding site was also determined. The 3' flanking region contained a small putative open reading frame (ORF) encoding a polypeptide of M_r 7022. The identity of this polypeptide remains to be elucidated. Comparisons of g1nA structural genes and GS enzymes at the nucleotide and amino acid levels between M. capsulatus (Bath) and both prokaryotes and eukaryotes have been determined. The presence of ntrA, ntrB, ntrC, g1nB and rpoD homologues in the M. capsulatus (Bath) genome was determined by heterologous hybridization studies. Type I and Type II obligate methanotrophs were also screened for g1nA, ntrC and ntrA homologues. Both Type I and Type II organisms were found to have homologues to each of these gene probes. A portion of a M. capsulatus (Bath) putative ntrC gene has been cloned on a cosmid, pCOS1 and was found to be unlinked to g1nA and lies some 8.5 kb downstream of g1nA. The development of a plasmid transformation and gene transfer system for M. capsulatus (Bath) based on previously published methods has also been assessed.
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Rudnick, Paul Anthony. "Studies on the regulatory mechanisms controlling nitrogenase synthesis and ammonia assimilation in Azotobacter vinelandiiand Sinorhizobium meliloti." Diss., The University of Arizona, 2001. http://hdl.handle.net/10150/279942.

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Biological nitrogen fixation (BNF) is the nitrogenase-catalyzed conversion of dinitrogen to ammonia by a select group of Bacteria and Archaea called diazotrophs. In turn, plants and other microbes assimilate ammonia during the synthesis of nucleic acids, proteins and other biomolecules. BNF is of special interest in agriculture where it replenishes soil nitrogen lost during repetitive farming. Basic knowledge of BNF might eventually lead to less dependence on expensive and polluting chemical fertilizers. For the studies presented here, two model diazotrophs, the free-living Azotobacter vinelandii , and the alfafa symbiont, Sinorhizobium meliloti, were used to investigate mechanisms controlling nitrogen fixation and nitrogen metabolism. In A. vinelandii, ammonia inhibits nitrogenase expression by limiting activity of the two-component activator, NifA; this involves the negatively acting sensor protein, NifL. Groundwork indicated that a global nitrogen-sensing system, present in many bacteria might control NifA activity since glnD mutants were unable to fix nitrogen. In other organisms, nitrogen limitation signals GlnD-mediated uridylylation of PII-like signal transduction proteins, which signals activation of a suite of genes involved in nitrogen source utilization. The goals of the current study were to characterize the operon encoding a PII-like protein in A. vinelandii, named GlnK, and determine its influence on NifA and nitrogen metabolism. The results indicated that glnK is an essential gene and that uridylylation of GlnK is required for activation of glutamine synthetase and NifA. Also presented here is evidence that GlnK interacts with NifL to stimulate its inhibitory properties. These results are consistent with a model in which uridylylation of GlnK in response to nitrogen limitation signals relief of NifL inhibition. In the last section of this dissertation, glnD of Sinorhizobium meliloti was cloned and sequenced because a PII-like protein had been previously implicated in control of nodule development and symbiosis. Unfortunately, S. meliloti glnD mutants could not be isolated unless glnD and flanking genes were provided in trans, indicating that the glnD operon is indispensable. These studies provide new insight into the global mechanisms controlling nitrogen fixation and metabolism and suggest that GlnD and PII-like proteins may regulate other targets, some of which are essential.
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Lee, Eva. "Investigation of a Commercial Product (BiOWiSH TM) for Nitrogen Management." DigitalCommons@CalPoly, 2012. https://digitalcommons.calpoly.edu/theses/721.

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Abstract Investigation of a Commercial Product (BiOWiSH­­TM) for Nitrogen Management Eva Lee BiOWiSH–Aqua, which has the capability of treating nitrogen from wastewater through bioaugmentation, is a commercial product consisting of a blend of microorganisms developed by BiOWiSH Technologies. A study of the treatment of nitrogen compounds (i.e. , , and ) using Biowish–Aqua was conducted using small scale experiments (flask experiments) and large scale experiments (column reactor experiments). In this work, column reactors were created to test Biowish–Aqua’s nitrogen treating capabilities by providing enough depth to simulate the dissolved oxygen gradient that can be observed in a pond. The results show that the optimal growth conditions for both ammonia assimilating and denitrifying bacteria are an anoxic environment with a carbon-to-nitrogen ratio of 2:1. Under this optimal growth environment, Biowish–Aqua was able to assimilate ammonia with a zero order k value of 3.06 ppm/day. Also, under the same conditions, Biowish–Aqua was able to eliminate nitrate ( ) and nitrite ( ) at a rate of 9.58 ppm/day and 5.64 ppm/day respectively. The experiments also suggested that with a C:N ratio of 2:1, Biowish–Aqua did not have an effect in slowing the hydrolysis of urea. Overall, this research suggests that the application of Biowish–Aqua is a feasible nitrogen removing strategy for wastewater with initial presence of ammonia and nitrate between 10 to 20 ppm. Keywords: Ammonia assimilation, Bioaugmentation, BiOWiSH, Denitrification
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Racher, Andrew John. "Studies on ammonium assimilation by Saccharomyces cerevisiae." Thesis, University of St Andrews, 1988. http://hdl.handle.net/10023/13996.

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Saccharomyces cerevisiae can assimilate ammonium by NADP-GDH or by GS-GOGAT. The aim of this project was to improve the efficiency of ammonium assimilation, and therefore substrate utilisation, of S. cerevisiae by elimination of the energy inefficient pathway (GS-GOGAT). GOGAT- mutants were isolated from a GDH- parent strain by their inability to use ammonium as sole nitrogen source. Two structural gene mutants were identified, one in each of the two structural genes encoding GOGAT. Constructs with different combinations of GDH- and GOGAT- mutations and corresponding wild type alleles were made, and their growth studied in medium supplemented with different levels of ammonium. The growth properties (as final culture density and growth rate) of GOGAT- and GOGAT+ strains transformed with the GDH1 gene, and grown with excess ammonium were very similar. It was concluded that, under the conditions used in this study, the loss of GOGAT does not improve the growth properties of the strain. Non-transformed constructs were grown with excess and limiting ammonium. Growth properties of the GDH- and GOGAT- strains suggest that GS-GOGAT functions in ammonium assimilation at very low ammonium levels. This conclusion needs further investigation because the GDH+ GOGAT- construct had lower NADP-GDH activity than the wild type. The physiology of ammonium assimilation by two industrial strains was compared to that of a laboratory wild type at different ammonium concentrations using shake-flask culture. All three strains possessed the three activities in MM+20mM NH4+, and the profiles of appearance/disappearance of activity were very similar. At lower ammonium concentrations, important differences between the strains became apparent. It is unclear if it is due to simple strain heterogeneity or represents significant differences between industrial and laboratory strains. On the basis of the enzyme data, GS-GOGAT appears to be important in ammonium assimilation by DCL1 at limiting concentrations.
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Bhatnagar, Lakshmi. "Contribution a l'étude du métabolisme du soufre et de l'azote chez deux archaebactéries méthanogènes." Paris 7, 1985. http://www.theses.fr/1985PA077102.

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Un appareillage simple et efficace a été mis au point pour la culture de bactéries méthanogènes. Grâce à ce système, la nutrition soufrée et azotée de Methanobacterium (Mb. ) ivanovi et de Mb. Thermoautotrophicum souche delta H a été étudiée. En présence de mercapto-2-éthanol, agent réducteur non métabolisé, il a été montré que, en plus du sulfure, Mb. Ivanovi utilise le soufre élémentaire, la cystéine, et la méthionine comme source de soufre et que Mb. Thermoautotrophicum utilise le soufre élémentaire et la cystéine. Les autres composés testés n'ont aucun effet (sulfate) ou inhibent (sulfite, thiosulfate et dithionite) la croissance et la méthanogénèse des deux souches. En ce qui concerne la nutrition azotée, outre l'ammoniaque, (i) seule la glutamine est utilisée comme source d'azote chez Mb. Ivanovi, (ii) la glutamine et l'urée sont utilisées comme sources Irazote chez Mb. Thermoautotrophicum. Les autres composés testés n'ont aucun effet (nitrate chez la souche delta H) ou inhibent (nitrite) la croissance et la méthanogénèse des deux souches. La mesure des activités glutamine synthétase (GS), glutamate synthase (GOGAT) et alanine déshydrogénase (ADH) chez les deux souches suggèrent l'existence d'une voie GS-GOGAT pour l'assimilation de l'ammoniaque. A des concentrations élevées d'ammoniaque l'ADH pourrait jouer un rôle. Par contre, la glutamate déshydrogénase n'a pas été détectée chez ces méthanogènes. La GS de Mb. Ivanovi a été purifiée à homogénéité. C'est un dodécamère d'environ 600. 000 daltons composé d'un seul type de sous-unité, thermostable et insensible à l'oxygène. Son pI est de 4,6. Son activité n'est pas régulée par un système d'adénylation mais elle est sensible à une inhibition allostérique. Aucune réaction croisée n'a été détectée entre la GS de Mb. Ivanovi et des anticorps contre la GS d'Escherichia coli, d'Anabaena 7120 ou de Bacillus megaterium. Des mutants de 141 ivanovi auxotrophes pour la glutamine ont été caractérisés. Ces mutants ont une activité GS fortement diminuée par rapport à celle de la souche sauvage. En utilisant la technique de Southern, de l'homologie a été mise en évidence entre les gènes de structure de la nitrogénase (nifHDK) de K. Pneumoniae et d'Anabaena et l'ADN de Mb. Ivanovi et Mb. Thermoautotrophicum. De plus, de l'homologie avec le gène de structure de la GS (glnA) d'Anabaena a été détectée dans l'ADN de Mb. Ivanovi.
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Rahman, Raja Noor Zaliha Raja Abd. "Studies on enzymes for ammonium assimilation in hyperthermophilic archaeon Pyrococcus sp. strain KOD1." Kyoto University, 1998. http://hdl.handle.net/2433/182328.

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Putra, Deddi Prima. "Étude du métabolisme azote des champignons ectomycorhiziens (Cenococcum geophilum, Scleroderma verrucosum) et des mycorhizes d'eucalyptus : optimisation et application des techniques de dosage des molécules anioniques et cationiques par électrophorèse capillaire." Nancy 1, 1996. http://www.theses.fr/1996NAN10129.

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Le métabolisme azoté a été étudié chez deux champignons ectomycorhiziens : l'ascomycète Cenococcum geophilum et le basidiomycète Scleroderma verrucosum ainsi que chez les ectomycorhizes constituées par S. Verrucosum et Eucalyptus diversicolor. Chez C. Geophilum, la méthionine sulfoximine (MSX) inhibe fortement l'activité de la glutamine synthétase (GS) et de la glutamate déshydrogénase à NADP (GDH à NADP). L'albizzine inhibe l'accumulation du glutamate ce qui indique une implication de la glutamate synthase (GOGAT). C. Geophilum assimile donc l'azote par la voie GDH à NADP et le cycle GS/GOGAT. S. Verrucosum utilise bien l'azote nitrique et la nitrate réductase est réprimé par l'ammonium. Les dosages enzymatiques montrent que le champignon assimile l'azote via le cycle GS/GOGAT et l'activité de la GDH à NADP est faible. Chez S. Verrucosum associé à E. Diversicolor, le 14C-glutamate est rapidement métabolisé en glutamine par la GS. Cependant, l'accumulation de l'aspartate et de l'alanine marque au 14C est importante, même en présence de MSX, indiquant la grande efficacité des aminotransférases de la plante-hôte pour fournir des acides aminés. Les champignons étudiés appartiennent par conséquent à deux groupes: l'un représenté par C. Geophilum qui possède la GDH à NADP et le cycle GS/GOGAT et l'autre représenté par S. Verrucosum qui ne possède que le cycle GS/GOGAT. L’utilisation de l'électrophorèse capillaire a permis la séparation des acides organiques, en particulier ceux du cycle de Krebs et la détermination de l'ammonium, du nitrate, d'acides aminés particuliers (desmosine, isodesmosine), ainsi que de nombreux autres anions et cations inorganiques
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Chadwick, Susan Glynnis. "A ¹⁵N study of the effects of nitrate, ammonium and nitrate plus ammonium nutrition on nitrogen assimilation in Hordeum vulgare." Master's thesis, University of Cape Town, 1985. http://hdl.handle.net/11427/22563.

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Bibliography: pages 98-112.
A review of the recent literature concerning the assimilation and utilisation of nitrate and ammonium nitrogen within plants has been presented. Barley plants (Hordeum vulgare L.cv. Clipper) were grown hydroponically under controlled environmental conditions. The aerated nutrient solutions contained 2mM inorganic ¹⁴N supplied as either nitrate alone, ammonium alone, a 1:1 nitrate plus ammonium mixture or a 3:1 nitrate plus ammonium mixture. After 20 days of growth ¹⁵N nutrient solutions were substituted. The plant material was harvested four and eight hours after the commencement of the ¹⁵N feeding experiment and prepared for analysis. Xylem sap was also collected for a period of one hour beginning half an hour before each harvest and continuing for half an hour after harvesting. Separate batches of plants were used for harvesting and sap collection. In nitrate-fed plants the shoot was shown to be the main organ of nitrate assimilation. Xylem sap analysis indicated that 66% of the ¹⁵N supply to the shoot was in the form of nitrate and the majority of the absorbed and assimilated ¹⁵N was located in this region. In ammonium-fed plants, however, ¹⁵N-ammonium accumulated in the root with only a very small amount detectable in the xylem sap. Some 93% of the ¹⁵N exported from root to shoot in the xylem stream was in the form of organic nitrogen (mainly glutamine). This indicated that the root was the major organ of ammonium assimilation and that the shoot was the main destination of root assimilated nitrogen.
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Books on the topic "Ammonia assimilation"

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Cardy, Donald Leonard Nicholas. The molecular biology of ammonia assimilation in the obligate methanotroph "Methylococcus capsulatus"strain Bath. [s.l.]: typescript, 1989.

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Peat, Lucinda Jane. The influence of notrogen nutrition on the cellular localisation of ammonia assimilation enzymes in barley (Hordeum vulgare L.cv Klaxon). Manchester: University of Manchester, 1996.

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Rasmussen, Patrick P. Hydrologic and water-quality conditions in the Kansas River, northeast Kansas, November 2001-August 2002, and simulation of ammonia assimilative capacity and bacteria transport during low flow. Reston, Va: U.S. Dept. of the Interior, U.S. Geological Survey, 2005.

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Rasmussen, Patrick P. Hydrologic and water-quality conditions in the Kansas River, northeast Kansas, November 2001-August 2002, and simulation of ammonia assimilative capacity and bacteria transport during low flow. Reston, Va: U.S. Dept. of the Interior, U.S. Geological Survey, 2005.

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Rasmussen, Patrick P. Hydrologic and water-quality conditions in the Kansas River, northeast Kansas, November 2001-August 2002, and simulation of ammonia assimilative capacity and bacteria transport during low flow. Reston, Va: U.S. Dept. of the Interior, U.S. Geological Survey, 2005.

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Rasmussen, Patrick P. Hydrologic and water-quality conditions in the Kansas River, northeast Kansas, November 2001-August 2002, and simulation of ammonia assimilative capacity and bacteria transport during low flow. Reston, Va: U.S. Dept. of the Interior, U.S. Geological Survey, 2005.

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Rasmussen, Patrick P. Hydrologic and water-quality conditions in the Kansas River, northeast Kansas, November 2001-August 2002, and simulation of ammonia assimilative capacity and bacteria transport during low flow. Reston, Va: U.S. Dept. of the Interior, U.S. Geological Survey, 2005.

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Rasmussen, Patrick P. Hydrologic and water-quality conditions in the Kansas River, northeast Kansas, November 2001-August 2002, and simulation of ammonia assimilative capacity and bacteria transport during low flow. Reston, Va: U.S. Dept. of the Interior, U.S. Geological Survey, 2005.

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G, Christensen Victoria, Kansas. Dept. of Health and Environment., and Geological Survey (U.S.), eds. Hydrologic and water-quality conditions in the Kansas River, northeast Kansas, November 2001-August 2002, and simulation of ammonia assimilative capacity and bacteria transport during low flow. Reston, Va: U.S. Dept. of the Interior, U.S. Geological Survey, 2005.

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Rasmussen, Patrick P. Hydrologic and water-quality conditions in the Kansas River, northeast Kansas, November 2001-August 2002, and simulation of ammonia assimilative capacity and bacteria transport during low flow. Reston, Va: U.S. Dept. of the Interior, U.S. Geological Survey, 2005.

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Book chapters on the topic "Ammonia assimilation"

1

Hirel, Bertrand, and Peter J. Lea. "Ammonia Assimilation." In Plant Nitrogen, 79–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-662-04064-5_4.

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van Heeswijk, Wally C., Hans V. Westerhoff, and Daniel Kahn. "Cascade Control of Ammonia Assimilation." In Modern Trends in Biothermokinetics, 397–99. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-2962-0_61.

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Evans, H. J., P. J. Bottomley, and W. E. Newton. "Carbon Metabolism and Ammonia Assimilation." In Nitrogen fixation research progress, 217–26. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5175-4_29.

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Rhodes, David, Dennis G. Brunk, and José R. Magalhães. "Assimilation Of Ammonia by Glutamate Dehydrogenase?" In Plant Nitrogen Metabolism, 191–226. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4613-0835-5_6.

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Lea, P. J., and R. D. Blackwell. "Ammonia assimilation, photorespiration and amino acid biosynthesis." In Photosynthesis and Production in a Changing Environment, 313–36. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1566-7_20.

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Lea, P. J., and R. D. Blackwell. "Ammonia assimilation photorespiration and amino acid biosynthesis." In Photosynthesis and Production in a Changing Environment, 313–36. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-010-9626-3_20.

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Cárdenas, J., F. J. Caballero, C. Moreno-Vivián, and F. Castillo. "Enzymology of Ammonia Assimilation in Purple Nonsulfur Bacteria." In Inorganic Nitrogen Metabolism, 148–53. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-71890-8_25.

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Schreier, Harold J. "Biosynthesis of Glutamine and Glutamate and the Assimilation of Ammonia." In Bacillus subtilis and Other Gram-Positive Bacteria, 281–98. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555818388.ch20.

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Kerby, N. W., P. Rowell, and A. Reglinski. "Ammonia Uptake and Assimilation by Mutant Strains of the Cyanobacterium Anabaena Variabilis Resistant to Ammonia-Analogues." In Inorganic Nitrogen in Plants and Microorganisms, 106–12. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-75812-6_16.

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Hirel, Bertrand, and Peter J. Lea. "The Biochemistry, Molecular Biology, and Genetic Manipulation of Primary Ammonia Assimilation." In Advances in Photosynthesis and Respiration, 71–92. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/0-306-48138-3_6.

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Reports on the topic "Ammonia assimilation"

1

Cavanaugh, Colleen M. Molecular Characterization and Regulation of Ammonia Assimilation in Chemoautotrophic Prokaryote-Eukaryote Symbioses. Fort Belvoir, VA: Defense Technical Information Center, July 1998. http://dx.doi.org/10.21236/ada350743.

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