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1

Fu, Jinxiang, Zhe Zhang, and Jinghai Zhu. "Study on the diversity of denitrification bacteria treating with wastewater by using PPGC filler on SBMBBR at low temperature." E3S Web of Conferences 158 (2020): 04002. http://dx.doi.org/10.1051/e3sconf/202015804002.

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Aiming at the problem of the low removal efficiency of biological nitrogen-removing of low temperature waste-water, using Polyurethane Porous Gel Carrier (PPGC)-SBMBBR treated low temperature sewage, in compared with conventional SBR,and viaing Miseq high-throughput sequencing technology in analysis of the differences of microbial diversity and abundance of structure on the two reactors of activated sludge, revealed dominant nitrogen-removing bacterium improving the treatment efficiency of low temperature sewage. The results shows that the removal efficiency of the effluent nitrogen and the sl
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2

Wei, Qun, Di Lv, Mei Hui Huang, De Shui Yu, and Jian Qiang You. "Research on Treatment of Domestic Sewage with Aerobic Denitrifying Bacteria Immobilized by Carbon Fiber." Applied Mechanics and Materials 675-677 (October 2014): 627–32. http://dx.doi.org/10.4028/www.scientific.net/amm.675-677.627.

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Aerobic denitrifying bacteria, a fast and efficient strain, is extensively adopted in the wastewater sphere, and immobilized aerobic denitrifying bacteria denitrification Technology, to some extent, solves many problems existing in the direct application of aerobic denitrifying bacteria. Concentrated sulfuric acid, nitric acid, potassium permanganate, ferric chloride etc. are used to pretreat carbon fiber respectively, and then the modified the carbon fiber is applied to fix the activated aerobic denitrifying bacteria to investigate the effects of the modification of the carbon fiber and the e
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3

Kang, Ai Bin, Ying Qiang Yao, and Yu Long Dong. "The Mechanism of Nitrogen Removal in Domestic Sewage of High Ammonia Nitrogen by Three-Step Series Constructed Rapid Infiltration System." Advanced Materials Research 356-360 (October 2011): 1248–52. http://dx.doi.org/10.4028/www.scientific.net/amr.356-360.1248.

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The removal and mechanism of ammonia nitrogen and total nitrogen were studie through three-step series of constructed rapid infiltration system by using high ammonia nitrogen domestic sewage of students' living area in a university. The result shows that the removal rate of ammonia nitrogen is 94.47% by using this system, which is 3% higher than conventional rapid infiltration system.The effluent can meet the standard Ⅰ—A of “Discharge standard of pollutants for municipal wastewater treatment plant(GB 18918-2002)”. The amount of ammonium oxidizing bacteria, nitrate oxidizing bacteria, nitrite
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4

Fesefeldt, A., K. Kloos, H. Bothe, H. Lemmer, and C. G. Gliesche. "Distribution of denitrification and nitrogen fixation genes inHyphomicrobiumspp. and other budding bacteria." Canadian Journal of Microbiology 44, no. 2 (1998): 181–86. http://dx.doi.org/10.1139/w97-139.

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Genomic DNA of Hyphomicrobium spp., Hirschia baltica ATCC 49814T, Hyphomonas oceanitis ATCC 33897T, and Pedomicrobium ferrugineum S-1290Twas investigated with gene probes specific for nitrate reductase (narG), cytochrome cd1containing nitrite reductase (nirS), Cu-containing nitrite reductase (nirK), nitrous oxide reductase (nosZ), ammonia monooxygenase (amoA), and nitrogenase reductase (nifH) by Southern or dot blot hybridization. The presence of denitrification genes could be demonstrated for Hyphomicrobium denitrificans 1869T, Hyphomicrobium aestuarii IFAM NQ-521GrT, Hyphomicrobium zavarzini
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5

Zhuang, Huichuan, Zhuoying Wu, Linji Xu, Shao-Yuan Leu, and Po-Heng Lee. "Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial Communities." Microorganisms 8, no. 6 (2020): 919. http://dx.doi.org/10.3390/microorganisms8060919.

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Single-stage nitrite shunt denitrification (through nitrite rather than nitrate) with low dissolved oxygen (DO) supply is a better alternative in terms of energy-efficiency, short-footprint, and low C/N-ratio requirement. This study investigates the optimal DO level with temperature effect, with saline sewage at the fixed hydraulic and solids retention times of 8 h and 8 d, respectively. Moreover, 16S rRNA gene sequencing analysis corresponding with total nitrogen (TN) and chemical oxygen demand (COD) removals in each operating condition were performed. Results showed that DO of 0.3 mg/L at 20
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6

Verbaendert, Ines, Paul De Vos, Nico Boon, and Kim Heylen. "Denitrification in Gram-positive bacteria: an underexplored trait." Biochemical Society Transactions 39, no. 1 (2011): 254–58. http://dx.doi.org/10.1042/bst0390254.

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Denitrifying organisms are essential in removing fixed nitrogen pollutants from ecosystems (e.g. sewage sludge). They can be detrimental (e.g. for agricultural soil) and can also produce the greenhouse gas N2O (nitrous oxide). Therefore a more comprehensive understanding of this process has become increasingly important regarding its global environmental impact. Even though bacterial genome sequencing projects may reveal new data, to date the denitrification abilities and features in Gram-positive bacteria are still poorly studied and understood. The present review evaluates current knowledge
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7

Ito, Tsukasa, Toru Aoi, Naoki Miyazato, et al. "Diversity and abundance of denitrifying bacteria in a simultaneously nitrifying and denitrifying rotating biological contactor treating real wastewater at low temperatures." H2Open Journal 2, no. 1 (2019): 58–70. http://dx.doi.org/10.2166/h2oj.2019.021.

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Abstract Nitrification and denitrification processes occur simultaneously in aerobic wastewater biofilms. Although wide regions of the world have average temperatures of less than 15 °C for a half year, few studies have investigated the nitrogen removal by nitrification and denitrification in a single-stage aerobic biofilm reactor used for treating real wastewater under low-temperature conditions. This study showed successful wastewater treatment in a high average nitrogen removal rate of 78% at low water temperatures by simultaneous nitrification and denitrification in a rotating biological c
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8

Cai, Xi, Kaili Li, Tengxia He, et al. "Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying Arthrobacter nicotianae D51 Strain in the Presence of Copper." Water 11, no. 3 (2019): 434. http://dx.doi.org/10.3390/w11030434.

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A heterotrophic nitrification and aerobic denitrification bacterium, strain D51, was identified as Arthrobacter nicotianae based on morphological, phospholipid fatty acids (PLFAs), and 16S rRNA gene sequence analyses. Further tests demonstrated that strain D51 had the capability to use nitrite, nitrate, or ammonium as the sole nitrogen source in the presence of Cu2+. The maximum removal efficiencies of nitrite, nitrate and ammonium were 68.97%, 78.32%, and 98.70%, respectively. Additionally, the maximum growth rate and denitrification capacity of this strain occurred in the presence of 0.05 mg
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9

Chen, Dongkai, Peizhen Chen, Xiangqun Zheng, Weimin Cheng, Qiang Wang, and Xiaocheng Wei. "Enhanced Denitrification of Integrated Sewage Treatment System by Supplementing Denitrifying Carbon Source." International Journal of Environmental Research and Public Health 18, no. 18 (2021): 9569. http://dx.doi.org/10.3390/ijerph18189569.

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Integrated sewage treatment system (ISTY) is a new technology for rural domestic sewage treatment. In the ISTY, the carbon source in the denitrification stage is often insufficient, affecting the denitrification efficiency. In order to improve the denitrification efficiency, several commonly available agricultural wastes, peanut shell (PS), sawdust (SD), peat (PT), and their mixtures (MT), were selected as supplementary carbon sources in the denitrification stage of ISTY to study the denitrification efficiency. Results show that PS exhibited a high carbon release capacity. PS released an enorm
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10

Li, Yong, Siyuan Zhao, Jiejie Zhang, Yang He, Jianqiang Zhang, and Rong Ge. "Screening and Diversity Analysis of Aerobic Denitrifying Phosphate Accumulating Bacteria Cultivated from A2O Activated Sludge." Processes 7, no. 11 (2019): 827. http://dx.doi.org/10.3390/pr7110827.

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The aerobic denitrifying phosphate accumulating bacteria (ADPB) use NO3− as an electron acceptor and remove nitrate by denitrification and concomitant uptake of excessive phosphorus in aerobic conditions. Activated sludge was collected from the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town, Chengdu City. The candidate ADPB strains were obtained by cultivation in the enriched denitrification media, followed by repeated isolation and purification on bromothymol blue (BTB) solid plates. The obtained candidates were further screened for ADPB strains by phosphorus uptake e
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11

Massone, A. G., K. Gernaey, H. Bogaert, A. Vanderhasselt, A. Rozzi, and W. Verstraete. "Biosensors for nitrogen control in wastewaters." Water Science and Technology 34, no. 1-2 (1996): 213–20. http://dx.doi.org/10.2166/wst.1996.0374.

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The control of nitrogen removal processes in activated sludge systems requires not only on-line values of the parameters such as ammonium, nitrate and nitrite, but also an evaluation on the nitrifying and denitrifying biomass activity, influent toxicity and carbon source requirements for denitrification. This paper describes four different biosensors, two on denitrification and two on nitrification. They all measure the biological activity of the nitrifying or denitrifying bacteria. Three of them measure nitrogen and carbon species, the fourth biosensor measures influent toxicity. These instru
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12

Cornish Shartau, Sabrina L., Marcy Yurkiw, Shiping Lin, et al. "Ammonium Concentrations in Produced Waters from a Mesothermic Oil Field Subjected to Nitrate Injection Decrease through Formation of Denitrifying Biomass and Anammox Activity." Applied and Environmental Microbiology 76, no. 15 (2010): 4977–87. http://dx.doi.org/10.1128/aem.00596-10.

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ABSTRACT Community analysis of a mesothermic oil field, subjected to continuous field-wide injection of nitrate to remove sulfide, with denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA genes indicated the presence of heterotrophic and sulfide-oxidizing, nitrate-reducing bacteria (hNRB and soNRB). These reduce nitrate by dissimilatory nitrate reduction to ammonium (e.g., Sulfurospirillum and Denitrovibrio) or by denitrification (e.g., Sulfurimonas, Arcobacter, and Thauera). Monitoring of ammonium concentrations in producing wells (PWs) indicated that denitrification was
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13

An, Yi, Keqiang Zhang, Lei Zhang, and Qi Dong. "Effect of passivated iron powder on final-product distribution in Fe-supported denitrification." Water Science and Technology 67, no. 8 (2013): 1664–70. http://dx.doi.org/10.2166/wst.2013.651.

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An integrated nitrate treatment using passivated iron powder (PIP) and Alcaligenes eutrophus, which is a kind of hydrogenotrophic denitrifying bacteria, was conducted to investigate the effect of iron oxide coating on final-product distribution in hydrogenotrophic denitrification. Based on the results, the autotrophic denitrification supported by PIP could completely remove about 50 mg·L−1 of nitrate within 4 days, and almost 80% of nitrate was changed into N2O (under acetylene blocking) without residual nitrite or ammonium. While only 53% of the nitrate was removed using acid-washed iron (AWI
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14

Araki, N., Y. Tsukamoto, A. Nagano, T. Yamaguchi, and H. Harada. "Real-time PCR quantification of nitrite reductase (nirS) genes in a nitrogen removing Fluidized Bed Reactor." Water Science and Technology 53, no. 6 (2006): 59–65. http://dx.doi.org/10.2166/wst.2006.171.

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Molecular approaches were applied to identify and enumerate denitrifying bacteria subsisting in a fluidized bed reactor (FBR). The FBR was continuously operated as a unit for the removal of nitrogen from the effluents of domestic sewage treatment plant, with an additional supply of methanol as a carbon source. By denaturing gradient gel electrophoresis (DGGE) and sequence analysis of 16S ribosomal RNA genes, Thauera group was found to be dominant among the denitrifying bacteria in the FBR sludge. Oligonucleotide probe THA155 for fluorescence in situ hybridization (FISH) was newly designed for
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15

Kampman, Christel, Laura Piai, Hardy Temmink, Tim L. G. Hendrickx, Grietje Zeeman, and Cees J. N. Buisman. "Effect of low concentrations of dissolved oxygen on the activity of denitrifying methanotrophic bacteria." Water Science and Technology 77, no. 11 (2018): 2589–97. http://dx.doi.org/10.2166/wst.2018.219.

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Abstract Chemical energy can be recovered from municipal wastewater as biogas through anaerobic treatment. Effluent from direct anaerobic wastewater treatment at low temperatures, however, still contains ammonium and considerable amounts of dissolved methane. After nitritation, methane can be used as electron donor for denitrification by the anaerobic bacterium ‘Candidatus Methylomirabilis oxyfera’. It was shown that in the presence of 0.7% O2, denitrifying methanotrophic activity slightly increased and returned to its original level after oxygen had been removed. At 1.1% O2, methane consumpti
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16

Dong, Li, Luo Yahong, Cai Yanan, Zeng Huiping, and Zhang Jie. "Bacterial composition and nutrient removal with a novel PIA-A2/O sewage treatment." Water Science and Technology 73, no. 11 (2016): 2722–30. http://dx.doi.org/10.2166/wst.2016.086.

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A novel post intermittent aeration anaerobic–anoxic–oxic (PIA-A2/O) process was developed to integrate shortcut nitrification–denitrification with denitrifying phosphorus (P) removal for domestic sewage treatment. With the transformation in configuration and phased start-up strategy, the nitritation rate and the ratio of denitrifying phosphorus accumulating organisms to phosphorus accumulating organisms (DPAO/PAO) were enhanced greatly to 88.2% and 83.9–91.7% in the PIA-A2/O process, respectively. Improved total nitrogen and phosphorus removal were achieved at long sludge retention time and lo
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17

Lin, Yen-Hui. "Nitrification/denitrification in swine wastewater using porous ceramic sticks with plastic rings as supporting media in two-stage fixed-biofilm reactors." Water Science and Technology 62, no. 5 (2010): 985–94. http://dx.doi.org/10.2166/wst.2010.347.

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This study evaluated the performance of oxic-anoxic fixed-biofilm reactors (FBRs) using porous ceramic sticks with plastic rings as supporting media for nitrogen and organic carbon (as COD) removal from swine wastewater. Experimental results indicate that the removal efficiency of NH4+-N increased to 86–92% from 52–98% as the volumetric ammonium-nitrogen loading rate increased to 0.25 kg NH4+-N/m3-d from 0.082 kg NH4+-N/m3-d. Furthermore, during the denitrifying column test, the average removal efficiencies for COD and NOx-N were 83 and 76%, respectively. Only small amounts of NO2−-N and NO3−-
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18

Huang, S., C. Chen, X. Yang, Q. Wu, and R. Zhang. "Distribution of typical denitrifying functional genes and diversity of the <i>nirS</i>-encoding bacterial community related to environmental characteristics of river sediments." Biogeosciences 8, no. 10 (2011): 3041–51. http://dx.doi.org/10.5194/bg-8-3041-2011.

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Abstract. Denitrification in river sediments leads to nitrate removal from the aquatic system; therefore, it is necessary to understand functional diversity of denitrifier communities in the system. Sediment samples (0–25 cm depth) were collected from three typical locations along the Pearl River. The real-time PCR approach was used to measure the abundance of nitrate (narG), nitrite (nirS, nirK and nrfA), and nitrous oxide (nosZ) reductase genes from the sediment samples. Assemblages of nirS, nirK and nosZ indicated that complete denitrification occurred in sediment cores, with the greatest n
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19

Zhao, Chen, Qu, Jin, Zheng, and Cui. "Insights into the Variations of Hao-Dependent Nitrifying and Nir-Dependent Denitrifying Microbial Communities in Ammonium-Graduated Lake Environments." Applied Sciences 9, no. 16 (2019): 3229. http://dx.doi.org/10.3390/app9163229.

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Biological nitrification and denitrification play significant roles in nitrogen-associated biogeochemical cycles. However, our understanding of the spatial scales at which microbial communities act and vary is limited. We used gene-specific metagenomic PCR to explore changes in nitrifying and denitrifying microbial communities within pristine lake and its branches, where the ammonium and dissolved organic carbon (DOC) concentrations form a gradient. The biomarkers hydroxylamine oxidoreductase and nitrite reductase genes indicated that strong relationships exist between the diversities and comm
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20

Boontian, Nittaya. "Effect of Zero Valent Iron (ZVI) in Wastewater Treatment: A Review." Applied Mechanics and Materials 775 (July 2015): 180–84. http://dx.doi.org/10.4028/www.scientific.net/amm.775.180.

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Cathodic hydrogen was produced in the presence of anaerobic zero valent iron (ZVI) corrosion by water. It can enhance microbial denitrification to convert nitrate to N2O and N2. Autotrophic denitrifying growth on ZVI can enhance nitrate removal. Results showed that by increasing nitrate removal rates, innocuous gases (N2O and N2) are produced rather than ammonium. Using steel wool with a small specific surface area instead of powdered ZVI, pH was not significantly increased. Little pH change was caused by corrosion. This is a positive condition for autotrophic denitrifying bacteria. ZVI was us
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21

Hao, X., H. J. Doddema, and J. W. van Groenestijn. "Use of contact tank to enhance denitrification in oxidation ditches." Water Science and Technology 34, no. 1-2 (1996): 195–202. http://dx.doi.org/10.2166/wst.1996.0372.

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Poor denitrification in a Pasveeer oxidation ditch is attributed to a lack of carbon sources available in the anoxic zone as it is essential to maintain a high C/N ratio for denitrification. Influent of sewage directly into the anoxic zone is not useful to maintain a high C/N ratio. The adsorptive capacity of activated sludge can rapidly increase the C/N ratio. Similar to a contact-stabilization process, a contact tank can be combined with the Pasveer ditch; it provides contact time (zone) between raw sewage and return sludge before entering the ditch. In principle, insoluble organic substrate
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22

Wałęga, Andrzej, Krzysztof Chmielowski, and Dariusz Młyński. "Influence of the Hybrid Sewage Treatment Plant’s Exploitation on Its Operation Effectiveness in Rural Areas." Sustainability 10, no. 8 (2018): 2689. http://dx.doi.org/10.3390/su10082689.

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The article evaluates the effectiveness of the removal of organic pollutants—nitrogen and phosphorus—from household sewage in a hybrid bioreactor with a submerged fixed bed. The experiment was carried out in two exploitation variants that were both conducted in a laboratory model of the hybrid bioreactor: (I) cycles of 120 min of aeration and 60 min of no aeration with a constant sewage dosage, and (II) cycles 60 min of aeration and 60 min of no aeration, with a periodic sewage dosage in the no-aeration phase. The experiment was carried out on real sewage primarily treated in a septic tank. Th
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23

Galland, William, Florence Piola, Céline Mathieu, Lyna Bouladra, Laurent Simon, and Feth el Zahar Haichar. "Does Biological Denitrification Inhibition (BDI) in the Field Induce an Increase in Plant Growth and Nutrition in Apium graveolens L. Grown for a Long Period?" Microorganisms 8, no. 8 (2020): 1204. http://dx.doi.org/10.3390/microorganisms8081204.

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Intensive agriculture uses a lot of nitrogen fertilizers to increase crop productivity. These crops are in competition with soil-denitrifying microorganisms that assimilate nitrogen in the form of nitrate and transform it into N2O, a greenhouse gas, or N2. However, certain plant species exude secondary metabolites, called procyanidins, which inhibit denitrifiers and increase the nitrate pool in the soil available for plant nutrition. This phenomenon is called biological denitrification inhibition. Previously, we showed that the addition of exogenous procyanidins to a lettuce crop induces denit
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24

Fu, Weide, Rencheng Zhu, Huanyun Lin, Yukai Zheng, and Zhanbo Hu. "Effect of organic concentration on biological activity and nitrogen removal performance in an anammox biofilm system." Water Science and Technology 84, no. 3 (2021): 725–36. http://dx.doi.org/10.2166/wst.2021.258.

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Abstract The effects of different concentrations of organic matter on the biological activity and nitrogen removal performance of the anaerobic ammonium oxidation (anammox) system was studied. The results showed that under the conditions of low influent total organic carbon (TOC ≤ 100 mg/L), the activity rate of anammox bacteria was basically unaffected, the anammox bacteria and denitrifying bacteria formed a good synergistic effect, and the maximum total nitrogen (TN) removal efficiency reached 95.77%. However, when the influent TOC concentration was up to 200 mg/L, the activity of anammox ba
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25

Zhang, Dandan, Li Cui, Hui Wang, and Jiyan Liang. "Study of sulfate-reducing ammonium oxidation process and its microbial community composition." Water Science and Technology 79, no. 1 (2019): 137–44. http://dx.doi.org/10.2166/wst.2019.027.

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Abstract In this study, the simultaneous removal of ammonium and sulfate was detected in a self-designed circulating flow reactor, in which ammonium oxidization was combined with sulfate reduction. The highest removal efficiencies of NH4+-N and SO42–S were 92% and 59.2%. NO2− and NO3− appeared in the effluent, and experimental studies showed that increasing the proportion of N/S in the influent would increase the NO2− concentration in the effluent. However, N/S [n(NH4+-N)/n(SO42–S)] conversion rates during the experiment were between 2.1 and 12.9, which may have been caused by the experiment's
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26

Tan, Chong, Fang Ma, and Shan Qiu. "Impact of carbon to nitrogen ratio on nitrogen removal at a low oxygen concentration in a sequencing batch biofilm reactor." Water Science and Technology 67, no. 3 (2013): 612–18. http://dx.doi.org/10.2166/wst.2012.554.

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A sequencing batch biofilm reactor (SBBR) filled with polyurethane (PU) was operated in low dissolved oxygen (DO) (0.1–0.9 mg/L) at three different carbon to nitrogen ratios (C/N ratios) (C/N = 1.8:1, 5.0:1 and 10.5:1) with focus on reactor performance and microbial community composition of nitrifying and denitrifying bacteria. Meanwhile, the ammonium, nitrite, nitrate, pH and dissolved oxygen were analyzed to monitor the process of nitrification-denitrification. The reactor had a steady ammonium removal in spite of a large variation in the initial ammonium loading (the ammonium removal effici
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27

Ona-Nguema, Georges, Delphine Guerbois, Céline Pallud, Jessica Brest, Mustapha Abdelmoula, and Guillaume Morin. "Biogenic Fe(II-III) Hydroxycarbonate Green Rust Enhances Nitrate Removal and Decreases Ammonium Selectivity during Heterotrophic Denitrification." Minerals 10, no. 9 (2020): 818. http://dx.doi.org/10.3390/min10090818.

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Nitrification-denitrification is the most widely used nitrogen removal process in wastewater treatment. However, this process can lead to undesirable nitrite accumulation and subsequent ammonium production. Biogenic Fe(II-III) hydroxycarbonate green rust has recently emerged as a candidate to reduce nitrite without ammonium production under abiotic conditions. The present study investigated whether biogenic iron(II-III) hydroxycarbonate green rust could also reduce nitrite to gaseous nitrogen during bacterial nitrate reduction. Our results showed that biogenic iron(II-III) hydroxycarbonate gre
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28

Mauffrey, Florian, Alexandra Cucaita, Philippe Constant, and Richard Villemur. "Denitrifying metabolism of the methylotrophic marine bacteriumMethylophaga nitratireducenticrescensstrain JAM1." PeerJ 5 (November 28, 2017): e4098. http://dx.doi.org/10.7717/peerj.4098.

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BackgroundMethylophaga nitratireducenticrescensstrain JAM1 is a methylotrophic, marine bacterium that was isolated from a denitrification reactor treating a closed-circuit seawater aquarium. It can sustain growth under anoxic conditions by reducing nitrate (${\mathrm{NO}}_{3}^{-}$) to nitrite (${\mathrm{NO}}_{2}^{-}$). These physiological traits are attributed to gene clusters that encode two dissimilatory nitrate reductases (Nar). Strain JAM1 also contains gene clusters encoding two nitric oxide (NO) reductases and one nitrous oxide (N2O) reductase, suggesting that NO and N2O can be reduced b
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29

Xu, Sai, Wenjing Lu, Muhammad Farooq Mustafa, et al. "Co-existence of Anaerobic Ammonium Oxidation Bacteria and Denitrifying Anaerobic Methane Oxidation Bacteria in Sewage Sludge: Community Diversity and Seasonal Dynamics." Microbial Ecology 74, no. 4 (2017): 832–40. http://dx.doi.org/10.1007/s00248-017-1015-x.

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30

Mielcarek, Artur, Kamila Ostrowska, Joanna Rodziewicz, Wojciech Janczukowicz, and Joanna Struk-Sokołowska. "Influence of temperature and C/N ratio on nitrifying and denitrifying bacteria of biofilters treating wastewater from de-icing airport runways." E3S Web of Conferences 116 (2019): 00050. http://dx.doi.org/10.1051/e3sconf/201911600050.

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One of the key elements in the operational management of airports is effective de-icing of surfaces. The run-off of precipitation water loaded with pollutants from de-icing is a considerable and costly problem. The aim of our research has been to evaluate the applicability of biofilters filled with light expanded clay aggregate (LECA) generated from incineration of sewage sludge and now serving as a matrix for the development of microorganisms. The tested biofilters treated precipitation water polluted with agents used to de-ice airports. The solution was tested on a laboratory scale, in a ran
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31

Bourbonnais, A., S. K. Juniper, D. A. Butterfield, et al. "Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge." Biogeosciences 9, no. 11 (2012): 4661–78. http://dx.doi.org/10.5194/bg-9-4661-2012.

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Abstract. Little is known about fixed nitrogen (N) transformation and elimination at diffuse hydrothermal vents where anoxic fluids are mixed with oxygenated crustal seawater prior to discharge. Oceanic N sinks that remove bio-available N ultimately affect chemosynthetic primary productivity in these ecosystems. Using 15N paired isotope techniques, we determined potential rates of fixed N loss pathways (denitrification, anammox) and dissimilatory nitrate reduction to ammonium (DNRA) in sulfidic hydrothermal vent fluids discharging from the subsurface at several sites at Axial Volcano and the E
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32

Cojean, Adeline N. Y., Jakob Zopfi, Alan Gerster, Claudia Frey, Fabio Lepori, and Moritz F. Lehmann. "Direct O<sub>2</sub> control on the partitioning between denitrification and dissimilatory nitrate reduction to ammonium in lake sediments." Biogeosciences 16, no. 23 (2019): 4705–18. http://dx.doi.org/10.5194/bg-16-4705-2019.

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Abstract. Lacustrine sediments are important sites of fixed-nitrogen (N) elimination through the reduction of nitrate to N2 by denitrifying bacteria, and they are thus critical for the mitigation of anthropogenic loading of fixed N in lakes. In contrast, dissimilatory nitrate reduction to ammonium (DNRA) retains bioavailable N within the system, promoting internal eutrophication. Both processes are thought to occur under oxygen-depleted conditions, but the exact O2 concentration thresholds particularly of DNRA inhibition are uncertain. In O2 manipulation laboratory experiments with dilute sedi
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33

Huang, S., C. Chen, Q. Wu, R. Zhang, and X. Yang. "Distribution of typical denitrifying functional genes and diversity of the <i>nirS</i>-encoding bacterial community related to environmental characteristics of river sediments." Biogeosciences Discussions 8, no. 3 (2011): 5251–80. http://dx.doi.org/10.5194/bgd-8-5251-2011.

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Abstract. Denitrification in river sediments leads to nitrate removal from the aquatic system; therefore, it is necessary to understand functional diversity of denitrifier communities in the system. Sediment samples (0–25 cm depth) were collected from three typical locations along the Pearl River. The real-time PCR approach was used to measure the abundance of nitrate (narG), nitrite (nirS, nirK and nrfA), and nitrous oxide (nosZ) reductase genes from the sediment samples. Assemblages of nirS, nirK and nosZ indicated that complete denitrification occurred in sediment cores, with the greatest n
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34

Bourbonnais, A., S. K. Juniper, D. A. Butterfield, et al. "Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge." Biogeosciences Discussions 9, no. 4 (2012): 4177–223. http://dx.doi.org/10.5194/bgd-9-4177-2012.

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Abstract. Little is known about nitrogen (N) transformations in general, and the elimination of N in particular, at diffuse vents where anoxic hydrothermal fluids have mixed with oxygenated crustal seawater prior to discharge. Oceanic N sinks that remove bio-available N ultimately affect chemosynthetic primary productivity in these ecosystems. Using 15N paired isotope techniques, we determined potential rates of fixed N-loss pathways (denitrification, anammox) and dissimilative nitrate reduction to ammonium (DNRA) in sulfidic hydrothermal vent fluids discharging from the subsurface at several
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35

Li, Shengpin, Guoxin Huang, Xiangke Kong, et al. "Ammonium removal from groundwater using a zeolite permeable reactive barrier: a pilot-scale demonstration." Water Science and Technology 70, no. 9 (2014): 1540–47. http://dx.doi.org/10.2166/wst.2014.411.

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In situ remediation of ammonium-contaminated groundwater is possible through a zeolite permeable reactive barrier (PRB); however, zeolite's finite sorption capacity limits the long-term field application of PRBs. In this paper, a pilot-scale PRB was designed to achieve sustainable use of zeolite in removing ammonium (NH4+-N) through sequential nitrification, adsorption, and denitrification. An oxygen-releasing compound was added to ensure aerobic conditions in the upper layers of the PRB where NH4+-N was microbially oxidized to nitrate. Any remaining NH4+-N was removed abiotically in the zeoli
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Shiwei, Cao, Jing Zhaoqian, Yuan Peng, Wang Yue, and Wang Yin. "Performance of constructed wetlands with different substrates for the treated effluent from municipal sewage plants." Journal of Water Reuse and Desalination 9, no. 4 (2019): 452–62. http://dx.doi.org/10.2166/wrd.2019.032.

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Abstract Constructed wetlands (CWs) are effective as an advanced treatment process for the treated effluent of municipal wastewater plants. An appropriate substrate, suitable macrophytes, and proper operation are crucial for pollutant abatement. In this research, three subsurface flow CWs with various substrates were investigated. Pollutants abatement efficiency under various operational schemes were analyzed. The results showed that the satisfactory hydraulic loading rate was 0.25 m3/(m2·d). When the C/N ratio of influent was adjusted to 5.87 by adding a carbon source, the denitrification and
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37

Penn, Justin L., Thomas Weber, Bonnie X. Chang, and Curtis Deutsch. "Microbial ecosystem dynamics drive fluctuating nitrogen loss in marine anoxic zones." Proceedings of the National Academy of Sciences 116, no. 15 (2019): 7220–25. http://dx.doi.org/10.1073/pnas.1818014116.

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The dynamics of nitrogen (N) loss in the ocean’s oxygen-deficient zones (ODZs) are thought to be driven by climate impacts on ocean circulation and biological productivity. Here we analyze a data-constrained model of the microbial ecosystem in an ODZ and find that species interactions drive fluctuations in local- and regional-scale rates of N loss, even in the absence of climate variability. By consuming O2to nanomolar levels, aerobic nitrifying microbes cede their competitive advantage for scarce forms of N to anaerobic denitrifying bacteria. Because anaerobes cannot sustain their own low-O2n
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Parravicini, V., K. Svardal, R. Hornek, and H. Kroiss. "Aeration of anaerobically digested sewage sludge for COD and nitrogen removal: optimization at large-scale." Water Science and Technology 57, no. 2 (2008): 257–64. http://dx.doi.org/10.2166/wst.2008.020.

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The paper will report about the experiences at an Austrian large wastewater treatment plant of 720,000 population equivalents, where anaerobically digested sewage sludge is further stabilised under aerobic conditions. Enhanced stabilisation of the anaerobically digested sludge was required at the plant in order to get a permit for landfill disposal of the dewatered stabilized sludge. By implementing a post-aeration treatment (SRT ∼ 6d; 36 °C) after anaerobic digestion the organic content of the anaerobically well digested sludge can be decreased by 16%. Investigations on site showed that durin
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Thomas, M., P. Wright, L. Blackall, V. Urbain, and J. Keller. "Optimisation of Noosa BNR plant to improve performance and reduce operating costs." Water Science and Technology 47, no. 12 (2003): 141–48. http://dx.doi.org/10.2166/wst.2003.0639.

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Noosa WWTP is publicly owned and privately operated by Australian Water Services. The process includes primary sedimentation, raw sludge fermentation, biological nutrient removal (BNR), sand filtration and ultraviolet (UV) disinfection. An innovative feature of the plant is the supplementary carbon dosing facility to avoid the use of metal salts (alum or ferric) for phosphorus removal. The average flow treated during 2000 was 9.0 ML/d. The annual 50 percentile effluent quality requirements for nutrients are total N &amp;lt; 5 mg/L and total P &amp;lt; 1 mg/L. The objectives of this project wer
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Maharjan, A. K., K. Mori, K. Nishida, and T. Toyama. "Nitrogen removal from ammonium-contaminated groundwater using dropping nitrification–cotton-based denitrification reactor." Water Supply, August 17, 2021. http://dx.doi.org/10.2166/ws.2021.258.

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Abstract A novel dropping nitrification–cotton-based denitrification reactor was developed for total nitrogen (N) removal from ammonium (NH4+)-contaminated groundwater. The nitrogen removal ability of the reactor was evaluated for 91 days. A 1 m long dropping nitrification unit was fed with synthetic groundwater containing 30 mg-NH4+-N/L at a flow rate of 2.16 L/d. The outlet of the dropping nitrification unit was connected to the cotton-based denitrification unit. The NH4+ present in the groundwater was completely oxidized (&amp;gt;90% nitrification efficiency) by nitrifying bacteria to nitri
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Sylla, Aboubacar, Aboussabiq Fatima Ezzahra, Hassou Najwa, et al. "DENITRIFICATION OF WASTEWATER TREATMENT PLANT EFFLUENT USING ANAEROBIC BACTERIAL BED REACTOR IMMERSED : OPERATING PERFORMANCE." Journal of Urban and Environmental Engineering, June 27, 2018, 208–18. http://dx.doi.org/10.4090/juee.2017.v11n2.208218.

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In this study, a heterotrophic denitrification was designed for domestic wastewater treatment with unexpected water flows at different loading rates. Benefited from excellent removal ability COD, shorten operating time and lower maintenance cost. During the time of operation (six months), injection of nitrate was made in the influent RALBI 1 while the RALBI 2 was fed with sewage without addition of nitrate. The COD concentration in the influent of the two reactors was the same. This two reactors (RALBI1 and RALBI2) were fed with real wastewater for 172 days at 6 h HRT, the average COD removal
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42

Suneethi, S., and Kurian Joseph. "Start up and Stabilization of Anammox Process in an Anaerobic Membrane Bioreactor (an MBR)." Linnaeus Eco-Tech, March 14, 2017, 117. http://dx.doi.org/10.15626/eco-tech.2010.012.

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Release of nitrate and ammonia rich wastewaters into the natural waters promotes eutrophication, aquatic toxicity and deterioration in water quality. Anaerobic Ammonium Oxidation (ANAMMOX) process is an advanced biological nitrogen removal alternative to traditional nitrification – denitrification, which removes ammonia using nitrite as the electron acceptor without oxygen. The feasibility to enrich ANAMMOX bacteria from anaerobic seed culture to start up an Anaerobic Membrane Bioreactor (An MBR) for N – removal is reported in this paper. The seed culture used was anaerobic digester sludge col
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Liu, Shuangyuan, Jingcheng Dai, Hehong Wei, et al. "Dissimilatory Nitrate Reduction to Ammonium (DNRA) and Denitrification Pathways Are Leveraged by Cyclic AMP Receptor Protein (CRP) Paralogues Based on Electron Donor/Acceptor Limitation in Shewanella loihica PV-4." Applied and Environmental Microbiology 87, no. 2 (2020). http://dx.doi.org/10.1128/aem.01964-20.

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ABSTRACT Under anoxic conditions, many bacteria, including Shewanella loihica strain PV-4, could use nitrate as an electron acceptor for dissimilatory nitrate reduction to ammonium (DNRA) and/or denitrification. Previous and current studies have shown that DNRA is favored under higher ambient carbon-to-nitrogen (C/N) ratios, whereas denitrification is upregulated under lower C/N ratios, which is consistent with our bioenergetics calculations. Interestingly, computational analyses indicate that the common cyclic AMP receptor protein (designated CRP1) and its paralogue CRP2 might both be involve
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44

Phanwilai, Supaporn, Pongsak Noophan, Chi-Wang Li, and Kwang-Ho Choo. "Effect of COD:N ratio on biological nitrogen removal using full-scale step-feed in municipal wastewater treatment plants." Sustainable Environment Research 30, no. 1 (2020). http://dx.doi.org/10.1186/s42834-020-00064-6.

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Abstract This study investigated the effect of low and high chemical oxygen demand (COD):N ratios on biological nitrogen removal and microbial distributions in full-scale step-feed (SF) municipal wastewater treatment plants (WWTPs) in Thailand (SF1) and Taiwan (SF2). The SF1 WWTP had a low COD:N (4:1) ratio, a long solids retention time (SRT) (&gt; 60 d), and low dissolved oxygen (DO) conditions (0.2 mg L− 1 in anoxic tank and 0.9 mg L− 1 in aerobic tank). The total nitrogen (TN) removal efficiency was 48%. The SF2 WWTP had a high COD:N (10:1) ratio, a short SRT (7 d), and high DO (0.6 mg L− 1
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Noophan, Pongsak, Rawiwan Rodpho, Pimook Sonmee, Martha Hahn, and Suthep Sirivitayaphakorn. "Nutrient Removal Performance on Domestic Wastewater Treatment Plants (Full Scale System) between Tropical Humid and Cold Climates." Applied Environmental Research, May 11, 2018, 32–39. http://dx.doi.org/10.35762/aer.2018.40.2.3.

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Two full scale systems of oxidation ditches for domestic wastewater treatment plants (WWTP) were used as study sites: Phuket Province, southern Thailand (representative of tropical humid climates) and Plum Creek, Castle Rock, Colorado, USA (representative of cold climates). The treatment systems at both sites were designed for biological nutrient removal (BNR) fromextended activated sludge. Nitrogen is removed by nitrification-denitrification processes. The solid retention time (SRT) for both treatment plants was ≥ 10 das recommended by theory for complete nitrification in activated sludge was
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46

Ahmad, Hafiz Adeel, Beibei Guo, Xuming Zhuang, et al. "A twilight for the complete nitrogen removal via synergistic partial-denitrification, anammox, and DNRA process." npj Clean Water 4, no. 1 (2021). http://dx.doi.org/10.1038/s41545-021-00122-5.

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AbstractThe prolonged start-up time and low total nitrogen removal of anammox hinder its full-scale application. Herein, application of nano-zerovalent iron and low strength magnetic field in sole and in combination to abbreviate the start-up period and long-term process stability of anammox was carried out. The reactor’s anammox start-up with the only magnetic field (R3) was 34 days, saving 43.3% time compared with the control. The increase of nitrogen removal efficiency over the control was 43.7% during the start-up period. However, the reactor with the coupled treatment of nano-zerovalent i
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Yue, Yihong, Ling Cai, Yi Tang, Yuanyuan Zhang, Ming Yang, and Fushun Wang. "Vertical Distribution of Bacterial Community in Water Columns of Reservoirs With Different Trophic Conditions During Thermal Stratification." Frontiers in Environmental Science 9 (April 9, 2021). http://dx.doi.org/10.3389/fenvs.2021.632089.

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Water eutrophication is a global ecological issue, and thermal stratification of water bodies can enable eutrophication. We examined bacterial communities in the stratified water columns and sediments in two different trophic reservoirs along the Wujiang River using quantitative real-time PCR and high-throughput sequencing. Bacterial 16S rRNA gene copies varied from 3.70 × 107 to 5.27 × 108 copies/L in the water column of Hongjiadu (HJD) Reservoir (60 m water depth) with slightly stratified variation; while in Wujiangdu (WJD) Reservoir (70 m water depth), bacterial abundance decreased markedly
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Duan, Yun-Feng, Xian-Wang Kong, Andreas Schramm, Rodrigo Labouriau, Jørgen Eriksen, and Søren O. Petersen. "Microbial N Transformations and N2O Emission after Simulated Grassland Cultivation: Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)." Applied and Environmental Microbiology 83, no. 1 (2016). http://dx.doi.org/10.1128/aem.02019-16.

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ABSTRACT Grassland cultivation can mobilize large pools of N in the soil, with the potential for N leaching and N2O emissions. Spraying with the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) before cultivation was simulated by use of soil columns in which the residue distribution corresponded to plowing or rotovation to study the effects of soil-residue contact on N transformations. DMPP was sprayed on aboveground parts of ryegrass and white clover plants before incorporation. During a 42-day incubation, soil mineral N dynamics, potential ammonia oxidation (PAO), denitrifying e
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