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Dissertations / Theses on the topic 'Amplification Methods'

Author: Grafiati

Published: 5 June 2025

Last updated: 15 July 2025

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1

King, George. "Studies of amplification methods in immunohistochemistry." Thesis, University of Aberdeen, 2001. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU537963.

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The aim of this project was to compare and contrast the results of three related methodologies which are of particular relevance or potential within our department -. a manually performed streptAvidin Biotin Complex (sABC/ HRP) method which for many years has served as our 'standard' immunocytochemical method; an integrated semi-automated systems from DAKO based on the TechMate 500 immunostainer and the ChemMate reagent package;. a manually performed tyramide amplification method regarded as amongst the most sensitive immunocytochemical method currently available. Antigen retrieval investigati

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2

McElfish, Erin Marie. "Methods for parallel amplification of single DNA molecules." Diss., [La Jolla] : University of California, San Diego, 2009. http://wwwlib.umi.com/cr/ucsd/fullcit?p1467961.

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Thesis (M.S.)--University of California, San Diego, 2009.<br>Title from first page of PDF file (viewed September 17, 2009). Available via ProQuest Digital Dissertations. Includes bibliographical references (p.76-77).

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3

Fernandez, Llenalia Garcia. "Statistical modelling of performance data for molecular amplification methods in diagnostic virology." Thesis, University of Abertay Dundee, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.650529.

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Nucleic Acid Technology (NAT), introduced in the late 90s, is a molecular amplification method that can be used for the diagnosis and management of patients with infectious diseases. NAT test results are obtained quicker and are quantified, providing great.er information than the positive/negative results available from traditional techniques. However, NATs arc technically demanding, susceptible to contamination and hence results from associated diagnostic tests may be inaccurate. External Quality Assessment (EQA) services are programmes developed to assess and advance the quality performance

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4

Thornley, John K. "Methods of application of piezoelectric multilayer actuators to high-speed clutching, using displacement amplification." Thesis, Loughborough University, 1993. https://dspace.lboro.ac.uk/2134/10851.

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The suitability of electromagnetic actuating devices for application to machines with ever more demanding response time specifications is discussed, with the proposal that piezoelectric actuator technology can produce practical devices with faster response times than solenoids, for example. This thesis discusses and validates the proposition that the performance of piezoelectric ceramic actuators makes them viable devices for inclusion in high-speed machine applications, where rapid clutching using two-state actuation is required. Further, techniques are devised and explored for the design and

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5

Civit, Pitarch Laia. "Development of electrochemical biosensors and solid-phase amplification methods for the detection of human papillomavirus genes." Doctoral thesis, Universitat Rovira i Virgili, 2012. http://hdl.handle.net/10803/84033.

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Un diagnòstic ràpid, precís i fiable és crucial per a la identificació d'una malaltia, com el càncer, on la detecció precoç pot millorar els resultants del tractament. El càncer de coll uterí és el tercer més comunament diagnosticat i la quarta causa de mort per càncer en les dones. La infecció persistent de virus del papil•loma humà d'alt risc (VPH) és la principal causa de càncer de coll uterí. Els biosensors electroquímics han rebut una important atenció a causa de les seves característiques. La seva compatibilitat amb tecnologies de microfabricació els fa atractius per al diagnòstic d'ADN

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6

Mignardi, Marco. "In situ Sequencing : Methods for spatially-resolved transcriptome analysis." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-110057.

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It is well known that cells in tissues display a large heterogeneity in gene expression due to differences in cell lineage origin and variation in the local environment at different sites in the tissue, a heterogeneity that is difficult to study by analyzing bulk RNA extracts from tissue. Recently, genome-wide transcriptome analysis technologies have enabled the analysis of this variation with single-cell resolution. In order to link the heterogeneity observed at molecular level with the morphological context of tissues, new methods are needed which achieve an additional level of information,

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7

Wirta, Valtteri. "Mining the transcriptome - methods and applications." Doctoral thesis, Stockholm : School of Biotechnology, Royal Institute of Technology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4115.

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8

Bernander, Sverker. "Detection and epidemiologic subtyping of Legionella pneumophila using DNA-based molecular methods /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-745-2.

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9

Nordén, Johan. "Assessment of methods for microRNA isolation, microRNA amplification, and development of a normalization strategy for sepsis biomarker research." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-18442.

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Sepsis, defined by organ dysfunction caused by an adverse immune response of the host to an infection, comes with considerable cost in human lives and as a substantial burden financially. Significant upgrades have been made over the past two decades when diagnosing and treating sepsis but still with room for improvements. Early detection is a cornerstone in the fight against sepsis, and the focus on strengthening diagnostics is in the forefront of modern research. The implementation of biomarkers may be the path of progression in this objective. This study aimed at establishing procedural foun

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10

Tombolato, Fabio. "Theoretical and computational methods for soft matter problems." Doctoral thesis, Università degli studi di Padova, 2008. http://hdl.handle.net/11577/3425989.

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The present thesis deals with the development and implementation of models for problems in soft condensed matter, at the interface between biology and material science. In particular, two projects have been carried out. The first focuses on the molecular origin of chirality in liquid crystalline solutions of semiflexible polyelectrolytes like DNA, columnar aggregates of deoxyguanosine tetramers (G-wires) and filamentous becteriophages. The second project deals with the integration between theoretical and experimental methods for the study of membrane-proteins systems, starting from the analys

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11

Landry, Matthew. "Analysis of Nanopore Detector Measurements using Machine Learning Methods, with Application to Single-Molecule Kinetics." ScholarWorks@UNO, 2007. http://scholarworks.uno.edu/td/533.

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At its core, a nanopore detector has a nanometer-scale biological membrane across which a voltage is applied. The voltage draws a DNA molecule into an á-hemolysin channel in the membrane. Consequently, a distinctive channel current blockade signal is created as the molecule flexes and interacts with the channel. This flexing of the molecule is characterized by different blockade levels in the channel current signal. Previous experiments have shown that a nanopore detector is sufficiently sensitive such that nearly identical DNA molecules were classified successfully using machine learning tech

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12

Boivin, Yannick. "New capacity design methods for seismic design of ductile RC shear walls." Thèse, Université de Sherbrooke, 2012. http://savoirs.usherbrooke.ca/handle/11143/1962.

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In order to produce economical seismic designs, the modern building codes allow reducing seismic design forces if the seismic force resisting system (SFRS) of a building is designed to develop an identified mechanism of inelastic lateral response. The capacity design aims to ensure that the inelastic mechanism develops as intended and no undesirable failure modes occur. Since the 1984 edition, this design approach is implemented in the Canadian Standards Association (CSA) standard A23.3 for seismic design of ductile reinforced concrete (RC) shear walls with the objectives of providing sufficie

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13

Bome-Mannathoko, Naledi Betsi. "Characterisation of Staphylococcus aureus from South West Wales : comparison of SCCmec-orfX amplification methods and genotyping of clinical isolates including Panton-Valentine Leukocidin-positive strains." Thesis, Swansea University, 2010. https://cronfa.swan.ac.uk/Record/cronfa42540.

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Methicillin-resistant Staphylococcus aureus is a leading cause of hospital infections world-wide. Consecutive S. aureus wound isolates (n=561) were collected from PHW Microbiology ABM Laboratory, Swansea (PHW-ABM); 137 (24.4%) were mecA-positive; 424 (75.6%) were mecA-negative using real-time PCR. Audit revealed that 15 (10.9%) mecA-positive strains were not reported as MRSA. Genotyping was performed using pulsed-field gel electrophoresis (PFGE), spa typing and SCCmec typing. MRSA predominantly belonged to EMRSA-15 (89.1% >) and EMRSA-16 (5.8%) clones. All S. aureus strains were included in an

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14

Lovmar, Lovisa. "Methods for Analysis of Disease Associated Genomic Sequence Variation." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4525.

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15

Jurečková, Nela. "PCR identifikace nepatogenních bakterií izolovaných ze sýrů." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2010. http://www.nusl.cz/ntk/nusl-216587.

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Different species of genus Bifidobacterium are part of human and animal intestinal flora. These bacteria have benefit effects and therefore they are used in foods and pharmaceutical products as probiotics. Cheese is now suitable as a probiotic matrix except yoghurts and fermentated milks. This diploma thesis was focused on optimalization of DNA isolation from bacteria of genus Bifidobacterium. Magnetic microparticles (P(HEMA-co¬-GMA)) were used for DNA isolation in presence of 8% polyethyleneglycol PEG 6000 and 5 M sodium chloride. Phenol extraction weas also used as an isolation method. Isola

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Konstantinidis, Michalis. "Preimplantation genetic diagnosis : new methods for the detection of genetic abnormalities in human preimplantation embryos." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:28611f65-7729-4293-9c3f-4fc3f0cc39d7.

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Preimplantation genetic diagnosis (PGD) refers to the testing of embryos produced through in vitro fertilization (IVF) in order to identify those unaffected by a specific genetic disorder or chromosomal abnormality. In this study, different methodologies were examined and developed for performance of PGD. Investigation of various whole genome amplification (WGA) methods identified multiple displacement amplification as a reliable method for genotyping single cells. Furthermore, this technology was shown to be compatible with subsequent analysis using single nucleotide polymorphism (SNP) microa

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17

Kerze, David James. "Performance Characteristics of an Innovative Wind Power System." Cleveland State University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=csu1203808948.

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18

Feng, Mabel Y. "Frequency translation method for low frequency variable gain amplification and filtering." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/41642.

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Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007.<br>Includes bibliographical references (leaves 75-78).<br>This thesis discusses an innovative solution to an industry challenge. A frequency translation method is designed to shift low frequency signals to intermediate frequencies in order to utilize higher-frequency components. This solution, appropriate for applications involving 1-10MHz signals, can provide continuously variable gain and filtering at little cost in dynamic performance. The working system converts the low freq

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19

Amara, Mohamed Kamel Eddine. "Applications de l'effet photoréfractif en métrologie optique : velocimetrie holographique par imagerie de particules & vélocimétrie Doppler laser." Rouen, 1998. http://www.theses.fr/1998ROUES019.

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La PIV permet de cartographier des distributions de vitesses dans les écoulements divers, flammes, jets, elle n'est cependant pas adaptée à l'étude de distributions de vitesses à trois composantes dans un volume d'étude. Des systèmes associant l'holographie à la PIV ont permis d'étendre l'étude aux volumes en utilisant des plaques photosensibles. Ils sont donc lents à cause du développement chimique et souffrent d'un RSB moyen. Dans la première partie de ce travail, un système de HPIV utilisant un cristal photoréfractif, support dynamique totalement recyclable, a été réalisé. Il se base sur l'

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20

De, Leon Ricardo 1957. "Use of gene probes and an amplification method for the detection of rotaviruses in water." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/191152.

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Rotaviruses are one of the most significant causes of diarrheal disease in the world. Their presence in groundwater and drinking water supplies constitutes a health risk to the population. The study of rotaviruses in the environment has been hampered by the lack of accessible and consistent detection methodologies. Gene probes and other molecular techniques are a novel approach for the detection of these viruses in water. The feasibility of these new techniques for the detection and study of rotaviruses in the environment has been assessed using the simian SA-11 and the culturable human Wa rot

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21

Sisman, Fatma Nurten. "Estimation Of Dynamic Soil Properties And Soil Amplification Ratios With Alternative Techniques." Master's thesis, METU, 2013. http://etd.lib.metu.edu.tr/upload/12615501/index.pdf.

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Earthquakes are among the most destructive natural disasters affecting urban populations. Structural damage caused by the earthquakes varies depending not only on the seismic source and propagation properties but also on the soil properties. The amplitude and frequency content of seismic shear waves reaching the earth&rsquo<br>s surface is dependent on local soil conditions. It is well known that the soft sediments on top of hard bedrock can greatly amplify the ground motion and cause severe structural damage. When the fundamental period of the soil is close to the fundamental period of a stru

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22

Clausson, Carl-Magnus. "Making Visible the Proximity Between Proteins." Doctoral thesis, Uppsala universitet, Science for Life Laboratory, SciLifeLab, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-217772.

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Genomic DNA is the template of life - the entity which is characterized by a self-sustaining anatomical development, regulated signaling processes, the ability to reproduce and to respond to stimuli. Through what is classically known as the central dogma, the genome is transcribed into mRNA, which in turn is translated into proteins. The proteins take part in most, if not all, cellular processes, and it is by unraveling these processes that we can begin to understand life and disease-causing mechanisms. In vitro and in vivo assays are two levels at which protein communication may be studied, a

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23

Yata, Tomoya. "Development of efficient amplification method of DNA hydrogel and composite-type DNA hydrogel for photothermal immunotherapy." 京都大学 (Kyoto University), 2016. http://hdl.handle.net/2433/215494.

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24

Chu, Yin Bui. "Evaluation of rapid method for detection of cytomegalovirus in clincal specimens using polymerase chain reaction DNA amplification." FIU Digital Commons, 1993. http://digitalcommons.fiu.edu/etd/2355.

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Human cytomegalovirus (HCMV) infection is the major cause of illness and death in immunocompromised patients. HCMV is the most common cause of congenital viral infection in humans. A polymerase chain reaction (PCR) method was developed for the rapid detection of CMV in urine. Several parameters of the PCR procedure were optimized to reduce time and improve sensitivity. By eliminating the extraction of DNA from clinical specimens, reducing the number of amplification cycles, utilization of the "hot start" PCR procedure and direct detection of PCR product by ethidium bromide fluorescence stainin

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25

Claesson, Cim. "Is Multiplex Ligation-dependent Probe Amplification a good method for screening formalin fixed paraffin embedded neuroblastoma tumors?" Thesis, Högskolan i Skövde, Institutionen för vård och natur, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-5442.

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Neuroblastoma is one of the most enigmatic solid tumors for scientists and pediatric oncologists. Neuroblastoma is primary a childhood form of cancer, consisting of neuroectodermal cells that originate from the neural crest and is destined for the adrenal medulla and sympathetic nervous system. The Neuroblastoma group at The University of Gothenburg received formalin-fixed paraffin-embedded tumor samples from Vietnam and this project was to examine if the quality of the DNA from, is good enough to run comparative genome hybridization array experiment on by using a cheaper technique Multiple

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26

Walsh, Helen Ann. "Rapid Detection of Grapevine Leafroll-associated virus Type 3 using the reverse transcription loop-mediated amplification method." Diss., University of Pretoria, 2013. http://hdl.handle.net/2263/41018.

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Grapevine Leafroll disease (GLD), one of the most destructive diseases of grapevines, has been found in every country where grapevines are grown. Grapevine Leafroll associated virus type 3 (GLRaV-3), one of several viruses associated with GLD globally, is the most prevalent virus in South African grapevines and therefore control of GLRaV-3 takes high priority in any strategy aimed at control of GLD. GLD can be controlled through the use of an integrated strategy which includes using certified plant material, controlling insect vectors through use of systemic insecticides and the removal

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27

Tan, Angela Y. C. "The development of an efficient method of mitochondrial DNA analysis." Monash University, Dept. of Forensic Medicine, 2003. http://arrow.monash.edu.au/hdl/1959.1/9525.

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28

Doule, Claude. "Amplification parametrique optique en regime femtoseconde et tomographie optique coherente : deux methodes d'imagerie proche infrarouge dans des milieux diffusants." Phd thesis, Université Paris Sud - Paris XI, 2000. http://pastel.archives-ouvertes.fr/pastel-00713989.

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Les travaux présents dans ce mémoire concernent l'imagerie par voie optique proche infrarouge dans un milieu qui diffuse la lumiere. L'application visee est une imagerie in vivo de l'intérieur des tissus biologiques. Deux techniques expérimentales différentes, opérant en configuration de retrodiffusion, ont ete développées. Leur principe commun est la sélection temporelle de la lumière revenant de l'objet éclaire. Pour la première technique, l'amplification paramétrique optique en régime femtoseconde a permis la réalisation d'une porte temporelle de 200 femtosecondes. Les images résolues tempo

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29

McKenna, James Patrick. "Development and clinical validation of a loop mediated isothermal amplification (LAMP) method for the rapid detection of Neisseria meningitidis." Thesis, Queen's University Belfast, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.602504.

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Loop mediated isothermal amplification (LAMP) is an innovative technique which allows the rapid detection of target nucleic acid sequences under isothermal conditions without the need for complex instrumentation. The development, systematic optimisation and clinical validation of a LAMP assay targeting the ctrA gene for the rapid detect ion of capsular N. meningitidis is described. Highly specific detection of capsular N. meningitidis type strains and clinical isolates was demonstrated, with no cross reactivity with other Neisseria spp., or with a comprehensive panel of other common human path

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30

Graciani, Guillaume. "Three-dimensional stochastic interferometry : theory and applications to high-sensitivity optical metrology and light scattering amplification Random dynamic interferometer:cavity amplified speckle spectroscopy using a highly symmetric coherent fieldcreated inside a closed Lambertian optical cavity 3D stochastic interferometry detects picometer dynamics of an optical volume Cavity Amplified Speckle Spectroscopy expands lightscattering methods to transparent or miniature samples Super-resolution provided by the arbitrarily strongsuperlinearity of the blackbody radiation." Thesis, Institut polytechnique de Paris, 2020. http://www.theses.fr/2020IPPAX058.

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La puissance de la métrologie optique nécessite généralement des géométries simples avec un alignement précis et une phase optique bien contrôlée. Dans cette thèse, nous développons plutôt la notion d'interférométrie du chaos, en utilisant un champ optique ayant un désordre géométrique maximal et une phase aléatoire. Nous montrons que la stochasticité conduit à une très grande sensibilité interférométrique et ouvre la possibilité d'un large éventail de nouvelles mesures optiques et d'une nouvelle méthode que nous appelons la Cavity Amplified Speckle Spectroscopy (Spectroscopie de Speckle Ampli

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Escalante, Maldonado Oscar Roberto. "Improvement of the quantitation method for the tdh+ Vibrio parahaemolyticus in molluscan shellfish based on most-probable- number, immunomagnetic separation, and loop-mediated isothermal amplification." Kyoto University, 2016. http://hdl.handle.net/2433/215445.

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Final publication is available at: http://journal.frontiersin.org/article/10.3389/fmicb.2015.00270/full<br>付記する学位プログラム名：グローバル生存学大学院連携プログラム<br>Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(医学)<br>甲第19619号<br>医博第4126号<br>新制||医||1015(附属図書館)<br>32655<br>京都大学大学院医学研究科医学専攻<br>(主査)教授中川一路, 教授木原正博, 教授松林公蔵<br>学位規則第4条第1項該当

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Abboud, Eddy. "Simulation, mastering, and reduction of vibrations and noises in electric car gearbox transmission systems." Thesis, Paris, HESAM, 2022. http://www.theses.fr/2022HESAE026.

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Les architectures actuelles des motorisations hybrides ou électriques des véhicules automobiles incluent des transmissions par engrenages en sortie du moteur électrique, pour adapter la vitesse de rotation à celle des roues du véhicule. Ces engrenages sont la source de vibrations qui se propagent par voie solidienne jusqu'au carter du système, qui se retrouve être la sources acoustique du bruit rayonné. Ces bruits affectent le confort de l'utilisateur, notamment car ils ne sont pas masqués par le bruit du moteur électrique, très différent de celui d'un moteur thermique classique. La maîtrise d

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Svahn, Fabian. "Like a Rolling Circle : Developing in-situ genotyping of chromosomal barcodes in the DuMPLING method." Thesis, Uppsala universitet, Molekylär systembiologi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-452675.

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DuMPLING is a newly developed high-throughput method to study singlecellphenotypes in a pooled and barcoded library using a microfluidicchip. The chip enables parallel biophysical measurements of singlecells, after which in-situ genotyping connects the cells to a certainstrain of the library. The method has been previously applied with abarcoded library, where genotyping was performed on barcodes presenton high copy number plasmids. In this project, I apply and developthe Rolling Circle Amplification method to amplify the signal frombarcodes present on the E. coli chromosome. A small libraryco

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Ahmad, Yaman Kayali. "Development of a Method for Detection of Shigatoxin-Producing Escherichia coli Belonging to Clinically Important Twelve O Serotypes Based on the Combination of PickPen-Assisted Immunomagnetic Separation and Loop-Mediated Isothermal Amplification." Kyoto University, 2015. http://hdl.handle.net/2433/199207.

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Gualberto, Felipe Augusto Souza. "Valor disgnóstico da nested PCR em tempo real em pacientes com meningite tuberculosa." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/5/5134/tde-26082014-093325/.

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Introdução: A meningite tuberculosa (MTB) é a forma mais grave e fatal de tuberculose. O diagnóstico oportuno e o tratamento adequado e precoce são os principais fatores associados com o bom prognóstico. Os métodos utilizados na prática médica diária - achados clínicos, exames de imagem e análise de líquido cefalorraquidiano (LCR) - têm baixa acurácia. A pesquisa do DNA do Mycobacterium tuberculosis no LCR através da reação em cadeia da polimerase (PCR, do inglês polimerase chain reaction) com a metodologia nested é promissora, especialmente quando associada à praticidade da amplificação do DN

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Boss, Marcel. "Entwicklung neuer Methoden zur Analytik von nicht-codierender RNA." Doctoral thesis, Humboldt-Universität zu Berlin, 2020. http://dx.doi.org/10.18452/21486.

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Ziel dieser Arbeit war die Entwicklung neuer Methoden zur Untersuchung zirkulärer RNA. Das erste Projekt dieser Arbeit beschäftigte sich mit der Erstellung eines universell einsetzbaren Protokolls zur Generierung einer funktionalisierten zirkulären RNA. Hierbei konnte zunächst erfolgreich eine Vorschrift zur Herstellung unmodifizierter circRNA etabliert werden. Im zweiten Schritt gelang auch die Generierung einer zirkulären RNA mit Alkin-Funktionalisierung. Geringe Ausbeuten gaben Anlass zur Entwicklung eines alternativen Verfahrens, bei dem die Zyklisierung von Kopf-Schwanz modifizierter RNA

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37

Amengual, Jennie. "The use of quantitative polymerase chain reaction amplification method to determine sex ratio of human spermatozoa in semen and to determine maternal contamination of amniotic fluid samples." FIU Digital Commons, 1996. http://digitalcommons.fiu.edu/etd/1278.

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We have modified a technique which uses a single pair of primer sets directed against homologous but distinct genes on the X and Y chromosomes, all of which are coamplified in the same reaction tube with trace amounts of radioactivity. The resulting bands are equal in length, yet distinguishable by restriction enzyme sites generating two independent bands, a 364 bp X-specific band and a 280 bp Y-specific band. A standard curve was generated to show the linear relationship between X/Y ratio average vs. %Y or %X chromosomal content. Of the 51 purified amniocyte DNA samples analyzed, 16 samples s

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Macé, Jean-Sébastien. "Modélisation du fonctionnement d’un gyrolaser He-Ne de très haute précision." Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112160/document.

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Les gyromètres laser He-Ne sont des senseurs inertiels dont la fiabilité et la précision sont reconnues depuis le milieu des années 1980. Leur grande sensibilité leur permet de mesurer des vitesses de rotation avec une précision qui atteint 10⁻³ °/ h dans le domaine aéronautique. Cependant, du fait d’un fonctionnement complexe basé sur une physique riche et variée, ses performances sont fortement dépendantes des conditions de fonctionnement et de toute modification du processus de fabrication. Dans ce cas, un travail de modélisation prend tout son sens, puisqu’il permet, outre une compréhensio

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Eulálio, Inês Mariana Cardoso. "Developmente of a CNVs detection method through qPCR." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22507.

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Mestrado em Biotecnologia<br>Os copy number variations (CNVs) consistem em segmentos de DNA de uma kilobase ou mais, que se encontram num número variável de cópias, em comparação com um genoma de referência. A deteção de CNVs é convencionalmente realizada através de técnicas de citogenética, como fluorescence in situ hybridization e array comparative genomic hybridization, ou com base em PCR, como multiplex ligation-dependent probe amplification, SNP arrays ou deep sequencing. Porém, a evolução da técnica de PCR quantitativo em tempo real (qPCR) permitiu que fosse, actualmente, considerada o m

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Fatemi, Hassan. "Investigation of the higher mode effects on the dynamic behaviour of reinforced concrete shear walls through a pseudo-dynamic hybrid test." Thèse, Université de Sherbrooke, 2017. http://hdl.handle.net/11143/11221.

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La plupart des bâtiments de moyenne et grande hauteur en béton armé sont munis de murs de refend ductiles afin résister aux charges latérales dues au vent et aux séismes. Les murs de refend ductiles sont conçus selon des règles conception stricts. Ces murs sont généralement conçus de façon à forcer la formation d’une rotule plastique à leur base dans l’éventualité d’un séismemajeur. Lors de la conception d’un mur, l’enveloppe des moments fléchissants ainsi que l’enveloppe des efforts tranchants dans la portion du mur situé au-dessus de la rotule plastique sont basés sur la résistance pro

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Saleh, Mona. "Entwicklung von Testsystemen auf der Basis der "Loop Mediated Isothermal Amplification (LAMP)" Methode zum Nachweis von Yersinia ruckeri, dem Erreger der Rotmaulseuche (ERM) und von Renibacterium salmoninarum, dem Erreger der bakteriellen Nierenkrankheit (BKD) der Salmoniden." Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-104831.

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SANTOS, Desiane Maiara Gomes dos. "Amplificação de pequenos sinais em osciladores parametricamente forçados." Universidade Federal de Campina Grande, 2015. http://dspace.sti.ufcg.edu.br:8080/jspui/handle/riufcg/1578.

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Submitted by Maria Medeiros (maria.dilva1@ufcg.edu.br) on 2018-08-29T14:12:32Z No. of bitstreams: 1 DESIANE MAIARA GOMES DOS SANTOS - DISSERTAÇÃO (PPGF) 2015.pdf: 6011160 bytes, checksum: a5021549766593cfe2eb8fe5314ea39b (MD5)<br>Made available in DSpace on 2018-08-29T14:12:32Z (GMT). No. of bitstreams: 1 DESIANE MAIARA GOMES DOS SANTOS - DISSERTAÇÃO (PPGF) 2015.pdf: 6011160 bytes, checksum: a5021549766593cfe2eb8fe5314ea39b (MD5) Previous issue date: 2015-04-10<br>Capes<br>Nesta dissertação, analisamos a dinâmica de osciladores parametricamente forçados, com enfoque na amplificação de peque

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Benmohamed, Fayçal. "Contribution au développement d'une méthode numérique pour étudier des structures planaires hyperfréquences." Thesis, Lyon, 2020. http://www.theses.fr/2020LYSES004.

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Le travail proposé est le résultat d’une collaboration entre le laboratoire Physique de la matière condensée et nanosciences de Monastir, le laboratoire Laplace de Toulouse et le laboratoire Hubert Curien de Saint-Étienne. Il s’inscrit dans un projet visant à étudier théoriquement la propagation des ondes électromagnétiques dans des dispositifs passifs à base du matériau magnétique anisotrope. La présence d’une couche magnétique dans telle structure a de nombreux avantages : non réciprocité́, performances intéressantes, isolation importante, …Plus précisément, c’est la fabrication des disp

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Bladh, Johan. "Hydropower generator and power system interaction." Doctoral thesis, Uppsala universitet, Elektricitetslära, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182188.

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After decades of routine operation, the hydropower industry faces new challenges. Large-scale integration of other renewable sources of generation in the power system accentuates the role of hydropower as a regulating resource. At the same time, an extensive reinvestment programme has commenced where many old components and apparatus are being refurbished or replaced. Introduction of new technical solutions in existing power plants requires good systems knowledge and careful consideration. Important tools for research, development and analysis are suitable mathematical models, numerical simula

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Marques, Filipe André da Palma. "Sensing methods for real-time Loop-mediated Isothermal Amplification in Digital Microfluidic systems." Master's thesis, 2018. http://hdl.handle.net/10362/58088.

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Digital Microfluidics (DMF) is a technology capable of maneuvering picoliter to microliter droplets in an independent and individual manner, with a wide variety of uses for bioassays and biosensing. These systems are advantageous for their small volumes, higher portability and multiplex assay capabilities, proving to be very capable of lab-on-chip and point-of-care applications. One of these applications are DNA amplification assays, of which, Loop Mediated Isothermal Amplification (LAMP), that has received increased interest from the scientific community. This method is a sensitive and simpl

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Huang, Chau-Chi, and 黃朝祺. "Development of DNA vaccine and methods for detecting duck circovirus by loop mediated isothermal amplification." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/64721679419733904321.

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碩士<br>國立中興大學<br>微生物暨公共衛生學研究所<br>99<br>Duck circovirus (DuCV) is an emerging disease in waterfowl. Infection with DuCV is associated with growth retardation and lymphoid depletion that leads immune suppression and increased susceptibility to second infections. Although diseases caused by DuCV might lead to economic loss, no vaccine is yet available for control of DuCV. Moreover, in the absence of method for growing DuCV in cell culture, polymerase chain reaction (PCR) is the major method for detection of this virus. The goal of this study is twofold; the first is to develop a DNA vaccine agains

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Upamanyu, Kapil. "Modelling, Stabilization Methods and Power Amplification for Power Hardware-in-Loop Simulation with Improved Accuracy." Thesis, 2022. https://etd.iisc.ac.in/handle/2005/6112.

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Accurate testing of a device under development, in near to real-life environment, is essential to the rapid growth of emerging technologies such as distributed generation, renewable energy sources, electrical storage, microgrid and electrical vehicles. Power hardware in loop (PHIL) simulation is an emerging methodology which allows for the testing of a physical hardware, i.e., a device under test (DUT), in a safe and controlled environment, without the rest of the system being available. The rest of the system for the DUT is emulated through its mathematical models computed in a real-tim

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Richards, Stephen Malone. "Investigation and application of methods for ancient DNA research." Thesis, 2015. http://hdl.handle.net/2440/100765.

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The introduction of high throughput sequencing (HTS) in 2005 caused a revolution in the field of ancient DNA (aDNA). Using the large sequencing capacity of HTS, researchers have overcome the abundant environmental contamination present in most aDNA extractions to reconstruct the genomes of long extinct organisms, such as an archaic horse that perished >500,000 years ago. The proliferation of genomes engendered by HTS has also led to the development of potential ancillary technologies for aDNA research such as genotyping microarrays. In this thesis, HTS and genotyping techniques were developed

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Bingham, James Edmund. "Comparison of RNA and DNA-based amplification methods for the discrimination of viable from non-viable Salmonella typhimurium." 2008. http://www.lib.ncsu.edu/theses/available/etd-07222008-131031/unrestricted/etd.pdf.

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楊琇涵. "Development of gold-nanoparticle-based and DNA machine amplification methods for the detection of Staphylococcus aureus protein A." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/69401507211421765699.

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