Relevant bibliographies by topics / Anaerobic incubation

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  4. Conference papers

Academic literature on the topic 'Anaerobic incubation'

Author: Grafiati
Published: 4 June 2021
Last updated: 9 February 2022

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Journal articles on the topic "Anaerobic incubation"

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Wiggs, Lois S., Joseph J. Cavallaro, and J. Michael Miller. "Evaluation of the Oxyrase OxyPlate Anaerobe Incubation System." Journal of Clinical Microbiology 38, no. 2 (2000): 499–507. http://dx.doi.org/10.1128/jcm.38.2.499-507.2000.

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The Oxyrase OxyPlate anaerobe incubation system was evaluated for its ability to support the growth of clinically significant anaerobic bacteria previously identified by the Anaerobe Reference Laboratory at the Centers for Disease Control and Prevention. The results were compared with those obtained with conventional anaerobe blood agar plates incubated in an anaerobe chamber. We tested 251 anaerobic bacterial strains. Plates were read at 24, 48, and 72 h; growth was scored by a numerical coding system that combines the degree of growth and the colony size. Organisms (number of strains tested) used in this study were Actinomyces (32),Anaerobiospirillum (8), Bacteroides(39), Campylobacter (8), Clostridium (96),Fusobacterium (12), Leptotrichia (8),Mobiluncus (8), Peptostreptococcus(16), and Propionibacterium (24). At 24 h, 101 (40.2%) of the 251 strains tested showed better growth with the anaerobe chamber than with the OxyPlate system, 10 (4.1%) showed better growth with the OxyPlate system, and the remaining 140 (55.8%) showed equal growth with both systems. At 48 h, 173 (68.9%) showed equal growth with both systems, while 78 (31.1%) showed better growth with the anaerobe chamber. At 72 h, 176 (70.1%) showed equal growth with both systems, while 75 (29.9%) showed better growth with the anaerobe chamber. The OxyPlate system performed well for the most commonly isolated anaerobes but was inadequate for some strains. These results indicate that the Oxyrase OxyPlate system was effective in creating an anaerobic atmosphere and supporting the growth of anaerobic bacteria within 72 h. OxyPlates would be a useful addition to the clinical microbiology laboratory lacking resources for traditional anaerobic culturing techniques.
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Rosadi, Maulana Yusup, Toshiro Yamada, Hudori Hudori, Hiroto Tamaoki, and Fusheng Li. "Characterization of dissolved organic matter extracted from water treatment sludge." Water Supply 20, no. 6 (June 9, 2020): 2194–205. http://dx.doi.org/10.2166/ws.2020.120.

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Abstract The characteristics of dissolved organic matter (DOM) that formed during the aerobic and anaerobic incubation of drinking water treatment sludge stored at different temperatures (5 °C, 20 °C, 40 °C) for long periods (7, 14, and 21 days) were investigated. Anaerobic incubation at high temperatures with prolonged storage was found to result in higher organic content than aerobic incubation (3.6–6.8 times at 40 °C). The high temperatures caused changes in the DOM fractions, with humic-like substances mainly formed in aerobic incubation and protein-like substances in anaerobic incubation. Results showed that the fluorescence intensity of humic-like and protein-like substances increased by 45% and 22%, respectively, at the end of the anaerobic incubation period. The UV-absorbing DOM constituents in aerobic incubation had lower molecular weights and were more heterogeneous than those in anaerobic incubation.
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Egwari, Louis Osayenum, Maria Olanike Buraimoh, and Nkiru Nneye Nwokoye. "Evaluation of two anaerobic systems for isolation of anaerobes." Microbiology Research 2, no. 1 (December 7, 2011): 24. http://dx.doi.org/10.4081/mr.2011.e24.

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Many systems are available for the isolation of anaerobic bacteria from clinical specimens. The jar system is the oldest and more adapted while the pouches are not popular with many investigators. The anaerobic chambers are expensive to maintain and technically inflexible. This study evaluated the efficacy of the Oxoid anaerobic jar and the GENbag pouches as anaerobic incubation systems. Anaerobic cultures were set up for 145 middle ear exudates and incubation was in the anaerobic jar, GENbag or a combination of both. The effect of specimen transport system and time lapse before culturing on the performance of the anaerobic systems were evaluated Ten genera of anaerobic bacteria were isolated with both systems (P &gt; 0.05). <em>Peptostreptococcus</em> and <em>Prevotella</em> were isolated more frequently in Oxoid jar than in GENbag (P &lt; 0.05) but both systems were not discriminatory for <em>Clostridium</em>, <em>Propionibacterium</em> and <em>Veillonella</em>. The use of GENbag as a backup to Oxoid jar increased isolation rate from 56.6% to 90.3% (P &gt; 0.05). Type of transport media or vehicle did not affect the recovery of anaerobes adversely as did delay in processing of specimen. A careful application of a number of variables may improve isolation of anaerobes from clinical specimens
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Metcalf, William W., Jun Kai Zhang, and Ralph S. Wolfe. "An Anaerobic, Intrachamber Incubator for Growth ofMethanosarcina spp. on Methanol-Containing Solid Media." Applied and Environmental Microbiology 64, no. 2 (February 1, 1998): 768–70. http://dx.doi.org/10.1128/aem.64.2.768-770.1998.

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ABSTRACT To simplify the incubation of Methanosarcina spp. on solid agar medium, a two-port, manual, rectangular air lock was modified to serve as an anaerobic incubator. In one operation, it is possible to incubate 153 petri plates, the equivalent of 11 standard anaerobic jars, with plating efficiencies identical to those of traditional protocols.
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Swerts, M., R. Merckx, and K. Vlassak. "Denitrification followed by N2-fixation during anaerobic incubation." Soil Biology and Biochemistry 28, no. 1 (January 1996): 127–29. http://dx.doi.org/10.1016/0038-0717(95)00126-3.

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Høiby, Martin, Arnfinn Aulie, and Per Ole Bjonnes. "Anaerobic metabolism in fowl embryos during normal incubation." Comparative Biochemistry and Physiology Part A: Physiology 86, no. 1 (January 1987): 91–94. http://dx.doi.org/10.1016/0300-9629(87)90282-9.

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Kirchmayr, R., H. E. Reichl, H. Schildorfer, R. Braun, and R. A. Somerville. "Prion protein: detection in ‘spiked’ anaerobic sludge and degradation experiments under anaerobic conditions." Water Science and Technology 53, no. 8 (April 1, 2006): 91–98. http://dx.doi.org/10.2166/wst.2006.239.

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The behavior of the transmissible spongiform encephalopathies (TSE) causing agent denominated “prion protein” in anaerobic sludge (biogas reactor) was assessed with incubation tests. A widely applied screening method for BSE in cattle on the basis of the Western blotting protocol was adapted to detect the Proteinase K resistant, scrapie-form prion protein (PrPSC). As PrPSC source homogenized TSE infected brain tissue of animals late in the clinical phase of disease was taken (301V/VM mouse-BSE; bovine BSE and 22A/SV mouse-scrapie). The incubation under mesophilic conditions did not show any significant reduction of the PrPSC titer. Under thermophilic conditions contradictory results were obtained. The reduction time of PrPSC in water was equal to or longer than the PrPSC reduction time in anaerobic sludge. In comparison, with sterilized (121 °C, steam pressure) or poisoned (sodium azide, 1% w/v) sludge used as incubation matrix a much shorter time resulted until no prion protein could be detected.
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RILEY, ANITA, DONG-HYUN KANG, and DANIEL Y. C. FUNG. "Comparison of Five Anaerobic Incubation Methods for Enumeration of Clostridium perfringens from Foods." Journal of Food Protection 62, no. 9 (September 1, 1999): 1041–44. http://dx.doi.org/10.4315/0362-028x-62.9.1041.

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This study compared the cost, speed, convenience, and sensitivity of five anaerobic systems. Fung's double-tube (FDT) method, the minitube method (MT), the sandwiched microtiter plate (SMP) method, and the Mitsubishi AnaeroPack System were compared with the Brewer anaerobic jar for total anaerobic bacterial counts in foods. Incubation was at 37°C for 4, 8, 12, and 24 h. The results indicated that FDT, MT, SMP, and the Mitsubishi AnaeroPack System were as sensitive as the Brewer anaerobic jar for the detection and enumeration of Clostridium perfringens from food products. The FDT, MT, and SMP methods recovered higher numbers of C. perfringens compared to the Brewer anaerobic jar (P &lt; 0.05) after 12 and 24 h incubation. The Brewer anaerobic jar was the most expensive method.
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Gupta, Akanksha, Arti Easwar, Yanice Maldonado, Pam Hamilton, Lori Baumgartner, and Jaber Aslanzadeh. "Assessment of Anaerobic Culture Incubation Time: A Tertiary Care Center Experience." American Journal of Clinical Pathology 152, Supplement_1 (September 11, 2019): S132—S133. http://dx.doi.org/10.1093/ajcp/aqz125.011.

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Abstract Background Currently, there is no consensus on the ideal incubation time for anaerobic cultures. A recent survey of CliniMicroNet reported anaerobic culture incubation times from 2 to 7 days. The goal of this study is to determine the ideal anaerobic culture incubation time and to retrospectively determine if longer incubation time affected management. Methods In this prospective study at Hartford Hospital, an 869-bed level I trauma center, 838 consecutive anaerobic cultures were Gram stained and planted on anaerobic blood, colistin and nalidixic acid (CNA), and kanamycin and vancomycin (KV) agar plates (BD Diagnostic Sparks). Plates were incubated in jars at 35°C and Anoxomat system (Advanced Instruments) was used to create anaerobic conditions. Plates were checked for growth on days 2, 3, 5, and 7. Although not all patient records (EPIC) were available, the type and duration of antibiotics were recorded for more than 100 records. Results Out of 74 cultures from bronchoalveolar lavage (1), bone (2), fluid (21), tissue (14), and wound (36), 53 grew 1 isolate, 15 grew 2 isolates, 5 grew 3 isolates, and 1 grew 4 isolates, for a total of 102 isolates. Sixty percent of isolates were Gram negative and 40% were Gram positive. Of these, 43% were detected on day 2, 21% on day 3, 20% on day 5, and 17% on day 7. In a majority of cases, broad-spectrum antibiotics (vancomycin/cefepime) were administered until culture results were obtained. Conclusion Overall, 73% Gram-negative isolates and 50% Gram-positive isolates were detected on day 2 or 3. Days 5 and 7 yielded 20% and 17% of all isolates, respectively. We conclude and corroborate with CLSI recommendations that anaerobic cultures should be incubated for at least 5 to 7 days.
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Wade, Mary Margaret, and Ying Zhang. "Anaerobic incubation conditions enhance pyrazinamide activity against Mycobacterium tuberculosis." Journal of Medical Microbiology 53, no. 8 (August 1, 2004): 769–73. http://dx.doi.org/10.1099/jmm.0.45639-0.

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Dissertations / Theses on the topic "Anaerobic incubation"

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Newton, Jennifer Denise. "Evidence for manganese-catalyzed nitrogen cycling in salt marsh sediments." Thesis, Available online, Georgia Institute of Technology, 2006, 2006. http://etd.gatech.edu/theses/available/etd-04072006-133610/.

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Thesis (M. S.)--Earth and Atmospheric Sciences, Georgia Institute of Technology, 2006.
Taillefert, Martial, Committee Chair ; Ingall, Ellery, Committee Member ; DiChristina, Thomas, Committee Member.
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Teixeira, Tânia Sofia Galego. "Previsão da mineralização do azoto a partir de solos e resíduos orgânicos." Master's thesis, 2008. http://hdl.handle.net/10400.5/631.

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Mestrado em Engenharia do Ambiente - Instituto Superior de Agronomia
With the objective to predict the availability of nitrogen (N) to the plants from the mineralization of soil organic matter and from organic residues applied to the soils, a quick and easy to perform waterlogged incubation experiment was developed, to investigate the mineralization of organic matter in 20 different soils, with and without application of two different organic residues (poultry manure and secondary pulp mill sludge). Soils tested, differed in texture, organic matter content as well as in pH and nutrients contents. A mild solubilizing agent (H2O) was used to extract easily mineralizable N both from soils and from mixtures of soils and residues. A ten days anaerobic incubation at 37ºC was performed for 10 days and sampled over this period. At each sampling time, a one hour extraction with 2M KCl was also done. A first-order kinetic model was fitted to the data, to predict N mineralization behavior in soils and mixtures. A quick chemical method using KCl 2M at 100ºC was also correlated to the model equations obtained, to better predict N mineralization. The results from both anaerobic incubation method and chemical extraction were promising in some soils, especially when soil texture was lighter. Poultry manure showed a similar mineralization pattern regardless of the soil to which it was added.
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Özyurt, Baris. "Identifikation von Genen und Mikroorganismen, die an der dissimilatorischen Fe(III)-Reduktion beteiligt sind." Doctoral thesis, 2009. http://hdl.handle.net/11858/00-1735-0000-0006-B66A-8.

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Book chapters on the topic "Anaerobic incubation"

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Swerts, M., R. Merckx, and K. Vlassak. "Influence of carbon availability on the production of NO, N2O, N2 and CO2 by soil cores during anaerobic incubation." In Progress in Nitrogen Cycling Studies, 633–39. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-011-5450-5_103.

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"Incubation Techniques for Anaerobic Bacteriology Specimens." In Clinical Microbiology Procedures Handbook, 4.4.1–4.4.4. Washington, DC, USA: ASM Press, 2016. http://dx.doi.org/10.1128/9781555818814.ch4.4.

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"Incubation Techniques for Anaerobic Bacteriology Specimens." In Clinical Microbiology Procedures Handbook, 3rd Edition, 709–12. American Society of Microbiology, 2010. http://dx.doi.org/10.1128/9781555817435.ch4.5.

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O’Farrell, Nigel. "Haemophilus ducreyi and chancroid." In Oxford Textbook of Medicine, 763–64. Oxford University Press, 2010. http://dx.doi.org/10.1093/med/9780199204854.003.070613_update_001.

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Haemophilus ducreyi is a Gram-negative, facultative anaerobic bacillus that is the cause of chancroid, which is endemic in sub-Saharan Africa and the Caribbean, although the overall global incidence of the condition has decreased dramatically since the mid 1990s. Clinical features—After an incubation period of 4 to 10 days, presentation is with a tender genital papule that develops into a pustule and then an ulcer with a ragged undermined edge and a yellow base that bleeds readily. The usual sites of infection in men are the prepuce and coronal sulcus, and in women the labia minora and fourchette. Inguinal lymphadenopathy is found in about half the male cases. Chancroid is an important risk factor for the transmission of HIV infection. HIV infection may result in atypical manifestations if chancroid....
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O’Farrell, Nigel. "Haemophilus ducreyi and chancroid." In Oxford Textbook of Medicine, edited by Christopher P. Conlon, 1071–72. Oxford University Press, 2020. http://dx.doi.org/10.1093/med/9780198746690.003.0118.

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Haemophilus ducreyi is a Gram-negative, facultative anaerobic bacillus that is the cause of chancroid. The condition was endemic in sub-Saharan Africa and the Caribbean, but the overall global incidence of the condition has decreased dramatically since the mid-1990s. After an incubation period of 4–10 days, presentation is with a tender genital papule that develops into a pustule and then an ulcer with a ragged undermined edge and a yellow base that bleeds readily. The usual sites of infection in men are the prepuce and coronal sulcus, and in women the labia minora and fourchette. Inguinal lymphadenopathy is found in about half the male cases. Chancroid is an important risk factor for the transmission of HIV infection. HIV infection may result in atypical manifestations of chancroid. Nucleic acid amplification tests are the optimal method of diagnosing H. ducreyi. Treatment is with either ciprofloxacin, erythromycin, azithromycin, or ceftriaxone.
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"The Development of Form and Function in Fishes and the Question of Larval Adaptation." In The Development of Form and Function in Fishes and the Question of Larval Adaptation, edited by Ian A. Johnston and Thomas E. Hall. American Fisheries Society, 2004. http://dx.doi.org/10.47886/9781888569582.ch5.

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<em>Abstract.</em>—Three phases of myogenesis have been identified in the myotomal muscles of larval teleosts. The commitment of embryonic slow and fast muscle lineages is determined prior to segmentation (embryonic myogenesis) and involves notochord and floorplate derived signaling pathways, which drive the adaxial cells to a slow muscle fate. The adaxial cells elongate to span the entire somite width and subsequently migrate through the myotome to form a superficial layer of slow muscle fibers. The remaining cells of the lateral mesoderm adopt the default fast muscle phenotype. The second phase of fiber expansion in the myotomes involves recruitment from discrete germinal zones for both slow and fast muscle fibers (stratified hyperplasia). Finally, myogenic precursor cells are activated throughout the myotome (mosaic hyperplasia). The progeny of these cells either fuse to form additional fibers on the surface of existing muscle fibers or are absorbed by fibers as they expand in diameter (hypertrophic growth). There is considerable species diversity with respect to the timing of innervation of the embryonic muscle fibers in relation to other developmental events, the degree of maturation of the muscle fibers at hatching, and the onset and relative importance of stratified and mosaic hyperplasia to growth during larval life. A subset of myogenic cells specified by their position in the anterior myotomes are thought to migrate out and populate the pectoral fin buds leading to the differentiation of the pectoral fin muscles. Little is known about the mechanism of formation of the unpaired fin muscles, which occurs after the differentiation of the myotomes and is often delayed until relatively late in larval life. During ontogeny, embryonic isoforms of the myofibrillar proteins are replaced by larval and adult isoforms, and the adult multiterminal pattern of slow muscle innervation gradually develops, reflecting changes in swimming style and performance as body size increases. The body length at which particular protein isoforms are switched on varies for each myofibrillar component and with temperature. In general, early larval stages show a greater reliance on aerobic metabolic pathways and a lower capacity for anaerobic glycolysis than later larval and juvenile stages. Temperature has a marked effect on the ultrastructure, number, and phenotype of larval muscle fibers. Recent evidence suggests that egg incubation temperature can influence myogenic cell commitment, producing long-term consequences for fiber recruitment and growth performance during subsequent stages of the life cycle. The ecological significance of the phenotypic plasticity of muscle growth and some potential applications to fisheries science are briefly discussed.
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Conference papers on the topic "Anaerobic incubation"

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Breica Borozan, Aurica, Despina-Maria Bordean, Gabriel Bujanca, Delia Dumbrava, and Sorina Popescu. "CONTROL OF PLANTS OF LOTUS CORNICULATUS L. ON AEROBIC AND ANAEROBIC FREE NITROGEN-FIXING BACTERIA." In GEOLINKS International Conference. SAIMA Consult Ltd, 2020. http://dx.doi.org/10.32008/geolinks2020/b1/v2/07.

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The free nitrogen fixing bacteria are able to mobilize important soil nutrients, transforming through biological processes the unusable molecular nitrogen into an active form and to improve soil fertility, influence many aspects of plant health and ensure their growth, showing interest for the scientific world and farmers. But, on the other hand, this bacterial segment may be influenced by the edaphic factors and the interconnection with the plants, the growth phase, the physiological state and the root system of the plant, by the root exudates, which demonstrates the importance of the bacterial community monitoring from the area of plants influence throughout the growing periods The aim of this study was to evaluate the influence of the age of the plants used as biofertilizer and soil moisture on the free nitrogen fixing bacterial communities (the genera Azotobacter and Clostridium) associated with the roots of the perennial plants of Lotus corniculatus L. There were two zones of interest, namely the area of influence of the roots of the plants (rhizosphere) but also the more distant area (edaphosphere). For the study of aerobic and anaerobic free nitrogen fixing bacteria soil samples were taken together with adjacent plants of Lotus corniculatus L. The experimental variants were located in the western part of Romania, the plants being cultivated on the same soil type, but on different plots, that were in the I-IV years of culture. The influence of Lotus corniculatus L. plants on the free nitrogen fixing bacteria has been reported in control experimental variants. Isolation and study of this bacterial group from the 8 experimental variants was performed on a specific mineral medium, favorable for the growth of the two bacterial genera. The results were evaluated after 5 and 10 days of incubation. Between the two assesments there were no noticeable differences in the nitrogen fixing bacterial community, except for the stimulatory effect observed in the control vatiant and rhizosphere of the first year culture. The plants influence on aerobic and anaerobic free nitrogen fixing bacteria was obvious in the II and IV years of the Lotus corniculatus L. culture, compared to the 76 control variants and varies substantially depending on the age of the plant. In most analyzed soil samples, both bacterial genera, Azotobacter and Clostridium were present, confirming the known ecological relation of unilateral advantage or passive stimulation of the aerobic bacteria compared to the anaerobic clostridia. Exceptions were the samples from the cultures of the first year (rhizosphere and control), but also the rhizosphere from the culture of the year II, where only anaerobic nitrogen fixing bacteria were detected. Our results suggested that plant-soil interactions exert control over the bacteria being studied.
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Nwaigwe, Kevin N., Abhishek Agarwal, and Emmanuel E. Anyanwu. "Biogas Potentials Evaluation of Household Wastes in Johannesburg Metropolitan Area Using the Automatic Methane Potential Test System (AMPTS) II." In ASME 2018 12th International Conference on Energy Sustainability collocated with the ASME 2018 Power Conference and the ASME 2018 Nuclear Forum. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/es2018-7553.

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A work on biogas potentials evaluation of household wastes in Johannesburg metropolitan area using the Automatic Methane Potential Test System (AMPTS) II is presented. The AMPTS II consists of three units — the sample incubation unit, CO2 absorption unit and the gas volume measuring device. Organic fraction of wastes collected from households within Johannesburg metropolis were sorted, ground and prepared into slurry by mixing with water. Microcrystalline cellulose powder with 3.5% loss on drying and 0.28g/cc density was used as control substrate while anaerobic sludge collected from a functional biogas reactor was used as inoculum. Anaerobic sludge was classified as sample A, household waste containing mainly non-food waste was labelled sample B, sample C was microcrystalline cellulose used as positive control while household waste composing of mainly food waste was classified as sample D. Each sample was fed into a 50 mL bottle reactor in triplicates and stirred in a clockwise direction continuously for 5 minutes with a pulse interval of 1 minute at a set temperature of 37°C for 30 days retention time. NaOH solution was prepared into solution following standard procedure and mixed with a prepared 0.4 % Thymolpthalein solution. The resultant solution was poured into the 100 mL bottles of the CO2 unit. Produced biogas was measured through water displacement in the volumetric bath and values read off through a data-logger connected to a laptop. Results indicated biochemical methane potential (BMP) of 69–800 NmL/gvs and biogas composition with more than 50% methane before CO2 fixing and over 80% after CO2 fixing. Given that the average amount of waste generated per person per day in South Africa is over 0.7 kg, there is huge potentials for biogas production from household wastes in Johannesburg.
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