Academic literature on the topic 'And Donkey Kong Jr'

AbstractCrossFit® is a competitive sport and fitness modality characterized by multiple physical capabilities and multi-joint movements. This study aimed to analyse and classify variables related to CrossFit® competitors’ specific performance. Fifteen male CrossFit® competitors were selected (n = 15; 30.57 ± 5.5 years; 1.76 ± 0.06 m; 78.55 ± 9.12 kg). Mean values were obtained for body mass index (25.3 ± 2.14 kg/m2), 4 skinfolds, 1 repetition maximum in the squat (137.60 ± 19.65 kg) and the bench press (101.67 ± 10.64 kg), maximum pull-ups (18.87 ± 5.05), sit-ups in 60 s (46.60 ± 4.22), peak power in the countermovement jump (3908.04 ± 423.68 W), VO2max with a shuttle run test (47.70 ± 4.79 ml kg-1·min-1), and time in the Workout of the Day (WOD) “Fran” (337.13 ± 119.19 s) and “Donkey Kong” (417.47 ± 98.44 s) components. Principal component analysis was conducted to classify variables and to select those most related to each new component (“strength and muscle mass”, “adiposity” and “aerobic capacity”). The correlation matrix was analysed, indicating significant correlations between “Donkey Kong” and VO2max (r = -.675; p “ .01), suprailiac skinfold (r = .713; p “ .01) and sit-ups (r = -.563; p “ .05); and between “Fran” and squat (r = -.528; p “ .05). Three important components characterizing CrossFit® competitors were identified: “strength and muscle mass”, low “adiposity” and “aerobic capacity”. Significant relationships between morphofunctional variables and Crossfit® performance were found in Crossfit® competitors.

Permainan atau yang sering disebut games merupakan salah satu aktivitas yang sering dilakukan olehkebanyakan orang dalam mencari hiburan dan kesenangan. Perkembangan games dari masa kemasa semakinberagam jenis dan aktivitasnya. Mulai dari games tradisonal yang dilakukan secara konvensional sampaidengan games yang dilakukan dengan teknologi.Dilihat dari perkembangan teknologi, games bukan lagisebagai aktivitas yang sulit untuk dimainkan, games dapat dimainkan dimana saja melalui perangkatteknologi apapun.Perkembangan games selain dari teknologi, juga sangat berkembang dari segi jenis/genredari games itu sendiri, yang menyesuaikan dengan kebutuhan pengguna/player. Berbagai genre game punmuncul, seperti genre action, adventure, RPG, simulasi, strategi dan lainnya.Pada penelitian kali ini penelitiberfokus kepada subgenre games action yaitu platform games. Beberapa platform games yang terkenal yaituMario bros, Jumping Flash, Donkey Kong. Games yang ringan tetapi memiliki unsur kesenangan danmenantang bagi yang memainkannya. Peneliti merasa tertarik dalam meneliti bagaimana membangun sebuahgame platform dengan teknik pengembangan game GDLC (Game Development Life Cycle), dengan modelperancangan game menggunakan model UML(Unifield Modeling Language). Game yang akandikembangkan yaitu game platform yang merupakan subgenre dari genre actiongames yang dapat dijalankanpada perangkat mobile phone berbasis android.

The disease caused by Influenza viruses has been well known for a very long time. In the recent period there has been noted an occurrence of pandemics caused by Influenza viruses type A with a high rate of mortality. The ongoing pandemic caused by avian influenza virus serotype H9N9 began in Hong Kong in 1992, and another pandemic caused by serotype H5N1 began in China (Hong Kong) in 1999. The world wide spreading of these viruses occurred due to migratory birds. Avian influenza was confirmed in Serbia in 2007. The goal of this study was to examine whether the avian influenza viruses type A circulate in the region of the Obedska bara marsh, which is a famous resort for many birds in Serbia, as well as many birds migrating from Europe to Africa and vice versa. The samples of blood sera of many animal species (123 samples from fowl, 64 samples from donkeys, 40 samples from horses) were tested by serologic reaction of inhibition of haemmaglutination (IHA) for the presence of antibodies to influenza A subtypes H5N1, H5N2, H5N3, H7N1 and H7N2. Also, the samples of blood sera of experimental chicken exposed to wild life in Obedska bara (sentinel species) were tested. Antibodies to subtypes H5N1, H5N2, H5N3, H7N1 and H7N2 were found in chicken from Dec, Boljevci, Petrovcic and Kupinovo villages but no antibodies were found in blood sera from hams from Dobanovci, Jakovo, Becmen and Surcin villages. From 23 samples from ducks antibodies were detected in 3 samples, and from 22 geese blood sera antibodies were found in 4 samples. From a total of 40 horse blood sera tested one was tested positive, and from 64 donkey sera 17 were positive for the presence of antibodies for avian influenza type A. In blood sera of experimental chicken antibodies were found by subtype H5N1 with corrections with H5N2 and H7N1.

In early July 2004, a severe crown rot of Canaan fir (Abies balsamea var. phanerolepis Fern.) was reported to the Virginia Cooperative Extension, Frederick County Office, and subsequently to the Virginia Tech Disease Clinic in Virginia Beach. One thousand five-year-old Canaan fir transplants (approximately 11 mm in caliper and 31 cm high) had been purchased from a tree nursery in Oregon and planted in the field in Frederick County, VA, in April of 2004. The field site had not been cultivated for 4 years after an apple orchard had been removed in 2000. By mid-May, needle browning had become serious, affecting the lower crown first. By August, transplants had suffered 40% mortality. Basal stems of affected plants were obviously discolored. Root and basal stem samples from several infected plants were then cultured on PARP-V8 agar on three different dates. Phytophthora sp. isolates were recovered from all stem samples but none from the roots. These isolates produced a large number of papillate sporangia that were caducous with short pedicels. Abundant oogonia with paragynous antheridia formed oospores directly on isolation plates within 7 days. The isolates were keyed as P. cactorum (2). This identification was confirmed using a single-strand-conformation polymorphism analysis of ribosomal DNA internal transcribed spacer (ITS)-1 (4). It appears that the source of inoculum was P. cactorum associated with the previous apple crop, since Canaan fir from the same transplant lot planted in a nearby field without a history of apples remained healthy. P. cactorum has been reported to cause root rot of noble fir (A. procera Rhedo), Pacific silver fir (A. amabilis (Dougl.) Forbes), and Shasta red fir (A. magnifica var. shastensis Lemm.) in the Pacific Northwest (3). It has also caused crown rot of Fraser fir (A. fraseri (Pursh) Poir.), noble fir, white fir (A. concolor (Gord. & Glend.) Lindl.), and balsam fir (A. balsamea (L.) Mill.) in Michigan (1). To our knowledge, this is the first report of P. cactorum attacking Canaan fir. Canaan fir currently is a recommended Christmas tree species for areas where Fraser fir does not do well due to root rot caused by Phytophthora cinnamomi. This study suggests that such a recommendation must be used with caution. Growing Canaan fir trees in P. cactorum-infested soil could result in devastating crop losses as reported in this note. References: (1) G. C. Adams, Jr. and A. Bielenin. Plant Dis. 72:79, 1988. (2) D. C. Erwin and O. K. Ribeiro. Phytophthora Diseases Worldwide. The American Phytopathological Society, St. Paul, MN, 1996. (3) P. B. Hamm and E. M. Hansen. Eur. J. For. Pathol. 12:167, 1982. (4) P. Kong et al. Fungal Genet. Biol. 39:238, 2003.

BackgroundMultiple genomics-based biomarkers of response to immune checkpoint inhibition have been reported or proposed, including tumor mutation/neoantigen frequency, PD-L1 expression, T cell receptor repertoire clonality, interferon gene signature expression, HLA expression, and others.1 Although genomics associations of response have been reported, the primary studies have used a variety of data generation and processing techniques. There is a need for data harmonization and assessment of generalizability of potential biomarkers across multiple datasets.MethodsWe acquired patient-level RNA sequencing FASTQ data files from 10 data sets reported in seven pan-cancer PD-1 and CTLA-4 immune checkpoint inhibition trials with matched clinical annotations.2–7 We applied a common bioinformatics workflow for quality control, mapping to reference (STAR), generating gene expression matrices (SALMON), T cell receptor repertoire inference (MiXCR), extraction of immune gene signatures and immune subtypes,8 and differential gene expression analysis (DESeq2). We analyzed i) immunogenomics features proposed as biomarkers, and ii) gene expression signatures built from each trial for association with overall survival across the set of trials using univariable Cox proportional hazards regression. In all, we assessed 9 total immunogenomics features/signatures. P-values were adjusted for multiple testing using the Benjamini-Hochberg method.ResultsOf the 9 immunogenomics features assessed, cytolytic activity score and expression of the Follicular Dendritic Cell Secreted Protein gene (FDCSP) were associated with survival in two of seven studies, respectively (adjusted p < 0.05) (figure 1). No proposed biomarkers were significantly associated with survival in more than two studies. The sets of genes significantly associated with clinical benefit across the studies were highly disjoint, with only three genes significant in three studies and thirteen genes significant in two studies (figure 2). No genes were significantly associated with clinical benefit in more than three of seven studies.Abstract 75 Figure 1Association of immunogenomics features and proposed biomarkers with survival in 10 publicly available datasets from 7 clinical trials with immune checkpoint blockade. Nine immunogenomics features were tested in 10 publicly available RNAseq data sets from 7 published clinical trials with immune checkpoint blockade for their correlation with outcome. SKCM, skin cutaneous melanoma; BLCA, bladder cancer; Kidney, kidney cancer; Ureter, ureteral cancer; GBM, glioblastomaAbstract 75 Figure 2Association of gene expression of single genes with survival in 10 publicly available datasets from 7 clinical trials with immune checkpoint inhibitorsConclusionsNo proposed biomarkers were highly generalizable across studies. We expect that integrated modeling incorporating multiple immunogenomics features will be required to build a robust and generalizable biomarker for ICI response. Further work is needed to analyze determinants of response and clinical benefit.AcknowledgementsWe would like to thank SITC for funding for this work as part of the Sparkathon TimIOS collaborative project.ReferencesZappasodi R, Wolchok JD, Merghoub T. Strategies for Predicting Response to Checkpoint Inhibitors. Curr Hematol Malig Rep 2018;13(5):383–95.Liu D, Schilling B, Liu D, Sucker A, Livingstone E, Jerby-Arnon L, Zimmer L, Gutzmer R, Satzger I, Loquai C, Grabbe S, Vokes N, Margolis CA, Conway J, He MX, Elmarakeby H, Dietlein F, Miao D, Tracy A, Gogas H, Goldinger SM, Utikal J, Blank CU, Rauschenberg R, von Bubnoff D, Krackhardt A, Weide B, Haferkamp S, Kiecker F, Izar B, Garraway L, Regev A, Flaherty K, Paschen A, Van Allen EM, Schadendorf D. Integrative molecular and clinical modeling of clinical outcomes to PD1 blockade in patients with metastatic melanoma. Nat Med 2019;25(12):1916–27.Gide TN, Quek C, Menzies AM, Tasker AT, Shang P, Holst J, Madore J, Lim SY, Velickovic R, Wongchenko M, Yan Y, Lo S, Carlino MS, Guminski A, Saw RPM, Pang A, McGuire HM, Palendira U, Thompson JF, Rizos H, Silva IPD, Batten M, Scolyer RA, Long GV, Wilmott JS. distinct immune cell populations define response to anti-pd-1 monotherapy and Anti-PD-1/Anti-CTLA-4 Combined Therapy. Cancer Cell 2019;35(2):238–55 e6.Cloughesy TF, Mochizuki AY, Orpilla JR, Hugo W, Lee AH, Davidson TB, Wang AC, Ellingson BM, Rytlewski JA, Sanders CM, Kawaguchi ES, Du L, Li G, Yong WH, Gaffey SC, Cohen AL, Mellinghoff IK, Lee EQ, Reardon DA, O’Brien BJ, Butowski NA, Nghiemphu PL, Clarke JL, Arrillaga-Romany IC, Colman H, Kaley TJ, de Groot JF, Liau LM, Wen PY, Prins RM. Neoadjuvant anti-PD-1 immunotherapy promotes a survival benefit with intratumoral and systemic immune responses in recurrent glioblastoma. Nat Med. 2019;25(3):477–86.Riaz N, Havel JJ, Makarov V, Desrichard A, Urba WJ, Sims JS, Hodi FS, Martin-Algarra S, Mandal R, Sharfman WH, Bhatia S, Hwu WJ, Gajewski TF, Slingluff CL, Jr., Chowell D, Kendall SM, Chang H, Shah R, Kuo F, Morris LGT, Sidhom JW, Schneck JP, Horak CE, Weinhold N, Chan TA. Tumor and microenvironment evolution during immunotherapy with nivolumab. Cell 2017;171(4):934–49 e16.Hugo W, Zaretsky JM, Sun L, Song C, Moreno BH, Hu-Lieskovan S, Berent-Maoz B, Pang J, Chmielowski B, Cherry G, Seja E, Lomeli S, Kong X, Kelley MC, Sosman JA, Johnson DB, Ribas A, Lo RS. Genomic and transcriptomic features of response to anti-PD-1 therapy in metastatic melanoma. Cell 2016;165(1):35–44.Rosenberg JE, Hoffman-Censits J, Powles T, van der Heijden MS, Balar AV, Necchi A, Dawson N, O’Donnell PH, Balmanoukian A, Loriot Y, Srinivas S, Retz MM, Grivas P, Joseph RW, Galsky MD, Fleming MT, Petrylak DP, Perez-Gracia JL, Burris HA, Castellano D, Canil C, Bellmunt J, Bajorin D, Nickles D, Bourgon R, Frampton GM, Cui N, Mariathasan S, Abidoye O, Fine GD, Dreicer R. Atezolizumab in patients with locally advanced and metastatic urothelial carcinoma who have progressed following treatment with platinum-based chemotherapy: a single-arm, multicentre, phase 2 trial. 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The enantioselective bromocyclization of dicarbonyl 1 to form dihydrofuran 3 using thiocarbamate catalyst 2 was developed (Angew. Chem. Int. Ed. 2013, 52, 8597) by Ying-Yeung Yeung at the National University of Singapore. Access to dihydrofuran 5 from the cyclic boronic acid 4 and salicylaldehyde via a morpholine-mediated Petasis borono-Mannich reaction was reported (Org. Lett. 2013, 15, 5944) by Xian-Jin Yang at East China University of Science and Technology and Jun Yang at the Shanghai Institute of Organic Chemistry. Chiral phosphoric acid 7 was shown (Angew. Chem. Int. Ed. 2013, 52, 13593) by Jianwei Sun at the Hong Kong University of Science and Technology to catalyze the enantioselective acetalization of diol 6 to form tetrahydrofuran 8 with high stereoselectivity. Jan Deska at the University of Cologne reported (Org. Lett. 2013, 15, 5998) the conversion of glutarate ether 9 to enantiopure tetrahy­drofuranone 10 by way of an enzymatic desymmetrization/oxonium ylide rearrange­ment sequence. Perali Ramu Sridhar at the University of Hyderabad demonstrated (Org. Lett. 2013, 15, 4474) the ring-contraction of spirocyclopropane tetrahydropyran 11 to produce tetrahydrofuran 12. Michael A. Kerr at the University of Western Ontario reported (Org. Lett. 2013, 15, 4838) that cyclopropane hemimalonate 13 underwent conver­sion to vinylbutanolide 14 in the presence of LiCl and Me₃N•HCl under microwave irradiation. Eric M. Ferreira at Colorado State University developed (J. Am. Chem. Soc. 2013, 135, 17266) the platinum-catalyzed bisheterocyclization of alkyne diol 15 to fur­nish the bisheterocycle 16. Chiral sulfur ylides such as 17, which can be synthesized easily and cheaply, were shown (J. Am. Chem. Soc. 2013, 135, 11951) by Eoghan M. McGarrigle at the University of Bristol and University College Dublin and Varinder K. Aggarwal at the University of Bristol to stereoselectively epoxidize a variety of alde­hydes, as exemplified by 18. The amine 20-catalyzed tandem heteroconjugate addition/Michael reaction of quinol 19 and cinnamaldehyde to produce bicycle 21 with very high ee was reported (Chem. Sci. 2013, 4, 2828) by Jeffrey S. Johnson at the University of North Carolina, Chapel Hill. Quinol ether 22 underwent facile photorearrangement–cycloaddition to 23 under irradiation, as reported (J. Am. Chem. Soc. 2013, 135, 17978) by John A. Porco, Jr. at Boston University and Corey R. J. Stephenson, now at the University of Michigan.