Academic literature on the topic 'And strand annealers'

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Journal articles on the topic "And strand annealers"

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Sugiman-Marangos, Seiji N., Yoni M. Weiss, and Murray S. Junop. "Mechanism for accurate, protein-assisted DNA annealing by Deinococcus radiodurans DdrB." Proceedings of the National Academy of Sciences 113, no. 16 (April 4, 2016): 4308–13. http://dx.doi.org/10.1073/pnas.1520847113.

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Accurate pairing of DNA strands is essential for repair of DNA double-strand breaks (DSBs). How cells achieve accurate annealing when large regions of single-strand DNA are unpaired has remained unclear despite many efforts focused on understanding proteins, which mediate this process. Here we report the crystal structure of a single-strand annealing protein [DdrB (DNA damage response B)] in complex with a partially annealed DNA intermediate to 2.2 Å. This structure and supporting biochemical data reveal a mechanism for accurate annealing involving DdrB-mediated proofreading of strand complementarity. DdrB promotes high-fidelity annealing by constraining specific bases from unauthorized association and only releases annealed duplex when bound strands are fully complementary. To our knowledge, this mechanism provides the first understanding for how cells achieve accurate, protein-assisted strand annealing under biological conditions that would otherwise favor misannealing.
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Chalapati, Sachin, Conor A. Crosbie, Dixita Limbachiya, and Nimesh Pinnamaneni. "Direct oligonucleotide sequencing with nanopores." Open Research Europe 1 (August 24, 2021): 47. http://dx.doi.org/10.12688/openreseurope.13578.2.

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Third-generation DNA sequencing has enabled sequencing of long, unamplified DNA fragments with minimal steps. Direct sequencing of ssDNA or RNA gives valuable insights like base-level modifications, phosphoramidite synthesis yield estimates and strand quality analysis, without the need to add the complimentary strand. Direct sequencing of single-stranded nucleic acid species is challenging as they are non-compatible to the double-stranded sequencing adapters used by manufacturers. The MinION platform from Oxford Nanopore Technologies performs sequencing by passing single-strands of DNA through a layer of biological nanopore sensors; although sequencing is performed on single-strands, the recommended template by the manufacturer is double-stranded. We have identified that the MinION platform can perform sequencing of short, single-strand oligonucleotides directly without amplification or second-strand synthesis by performing a single annealing step before library preparation. Short 5’ phosphorylated oligos when annealed to an adapter sequence can be directly sequenced in the 5' to 3' direction via nanopores. Adapter sequences were designed to bind to the 5’ end of the oligos and to leave a 3’ adenosine overhang after binding to their target. The 3’ adenosine overhang of the adapter and the terminal phosphate makes the 5’ end of the oligo analogous to an end-prepared dsDNA, rendering it compatible with ligation-based library preparation for sequencing. An oligo-pool containing 42,000, 120 nt orthogonal sequences was phosphorylated and sequenced using this method and ~90% of these sequences were recovered with high accuracy using BLAST. In the nanopore raw data, we have identified that empty signals can be wrongly identified as a valid read by the MinION platform and sometimes multiple signals containing several strands can be fused into a single raw sequence file due to segmentation faults in the software. This direct oligonucleotide sequencing method enables novel applications in DNA data storage systems where short oligonucleotides are the primary information carriers.
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Chalapati, Sachin, Conor A. Crosbie, Dixita Limbachiya, and Nimesh Pinnamaneni. "Direct oligonucleotide sequencing with nanopores." Open Research Europe 1 (May 12, 2021): 47. http://dx.doi.org/10.12688/openreseurope.13578.1.

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Third-generation DNA sequencing has enabled sequencing of long, unamplified DNA fragments with minimal steps. Direct sequencing of ssDNA or RNA gives valuable insights like base-level modifications, phosphoramidite synthesis yield estimates and strand quality analysis, without the need to add the complimentary strand. Direct sequencing of single-stranded nucleic acid species is challenging as they are non-compatible to the double-stranded sequencing adapters used by manufacturers. The MinION platform from Oxford Nanopore Technologies performs sequencing by passing single-strands of DNA through a layer of biological nanopore sensors; although sequencing is performed on single-strands, the recommended template by the manufacturer is double-stranded. We have identified that the MinION platform can perform sequencing of short, single-strand oligonucleotides directly without amplification or second-strand synthesis by performing a single annealing step before library preparation. Short 5’ phosphorylated oligos when annealed to an adapter sequence can be directly sequenced in the 5' to 3' direction via nanopores. Adapter sequences were designed to bind to the 5’ end of the oligos and to leave a 3’ adenosine overhang after binding to their target. The 3’ adenosine overhang of the adapter and the terminal phosphate makes the 5’ end of the oligo analogous to an end-prepared dsDNA, rendering it compatible with ligation-based library preparation for sequencing. An oligo-pool containing 42,000, 120 nt orthogonal sequences was phosphorylated and sequenced using this method and ~90% of these sequences were recovered with high accuracy using BLAST. In the nanopore raw data, we have identified that empty signals can be wrongly identified as a valid read by the MinION platform and sometimes multiple signals containing several strands can be fused into a single raw sequence file due to segmentation faults in the software. This direct oligonucleotide sequencing method enables novel applications in DNA data storage systems where short oligonucleotides are the primary information carriers.
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Bartos, Jeremy D., Wensheng Wang, Jason E. Pike, and Robert A. Bambara. "Mechanisms by Which Bloom Protein Can Disrupt Recombination Intermediates of Okazaki Fragment Maturation." Journal of Biological Chemistry 281, no. 43 (August 31, 2006): 32227–39. http://dx.doi.org/10.1074/jbc.m606310200.

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Bloom syndrome is a familial genetic disorder associated with sunlight sensitivity and a high predisposition to cancers. The mutated gene, Bloom protein (BLM), encodes a DNA helicase that functions in genome maintenance via roles in recombination repair and resolution of recombination structures. We designed substrates representing illegitimate recombination intermediates formed when a displaced DNA flap generated during maturation of Okazaki fragments escapes cleavage by flap endonuclease-1 and anneals to a complementary ectopic DNA site. Results show that displaced, replication protein A (RPA)-coated flaps could readily bind and ligate at the complementary site to initiate recombination. RPA also displayed a strand-annealing activity that hastens the rate of recombination intermediate formation. BLM helicase activity could directly disrupt annealing at the ectopic site and promote flap endonuclease-1 cleavage. Additionally, BLM has its own strand-annealing and strand-exchange activities. RPA inhibited the BLM strand-annealing activity, thereby promoting helicase activity and complex dissolution. BLM strand exchange could readily dissociate invading flaps, e.g. in a D-loop, if the exchange step did not involve annealing of RPA-coated strands. Use of ATP to activate the helicase function did not aid flap displacement by exchange, suggesting that this is a helicase-independent mechanism of complex dissociation. When RPA could bind, it displayed its own strand-exchange activity. We interpret these results to explain how BLM is well equipped to deal with alternative recombination intermediate structures.
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Capitani, Gian Carlo, Jean-Claude Doukhan, Thomas Malcherek, and Michael Carpenter. "Strain modulation around inclusions in an annealed natural cordierite." European Journal of Mineralogy 13, no. 5 (September 27, 2001): 921–28. http://dx.doi.org/10.1127/0935-1221/2001/0013-0921.

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Bilang, R., A. Peterhans, A. Bogucki, and J. Paszkowski. "Single-stranded DNA as a recombination substrate in plants as assessed by stable and transient recombination assays." Molecular and Cellular Biology 12, no. 1 (January 1992): 329–36. http://dx.doi.org/10.1128/mcb.12.1.329.

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Two separate assays, one that requires stable integration of recombination products and one that does not, were employed to elucidate the role of single-stranded DNA in extrachromosomal homologous recombination in Nicotiana tabacum. Both assays revealed that single-stranded DNA in linear and in circular forms was an efficient substrate for recombination, provided that the cotransformed recombination substrates were of complementary sequence, so that direct annealing was possible. Recombination was inefficient when both single-stranded recombination partners contained homologous regions of identical sequence and generation of a double-stranded DNA was required prior to heteroduplex formation. These results indicate that direct annealing of single strands is an important initial step for intermolecular recombination in tobacco cells. Annealed cotransformed single-stranded molecules yielded intermediates that could be further processed by either continuous or discontinuous second-strand synthesis. The type of intermediate had no influence on the recombination efficiency. Double-stranded circles were unable to recombine efficiently either with each other or with single-stranded DNA. Our results suggest that a helicase activity is involved in the initial steps of double-stranded DNA recombination which unwinds duplex molecules at the site of double-strand breaks.
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Bilang, R., A. Peterhans, A. Bogucki, and J. Paszkowski. "Single-stranded DNA as a recombination substrate in plants as assessed by stable and transient recombination assays." Molecular and Cellular Biology 12, no. 1 (January 1992): 329–36. http://dx.doi.org/10.1128/mcb.12.1.329-336.1992.

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Two separate assays, one that requires stable integration of recombination products and one that does not, were employed to elucidate the role of single-stranded DNA in extrachromosomal homologous recombination in Nicotiana tabacum. Both assays revealed that single-stranded DNA in linear and in circular forms was an efficient substrate for recombination, provided that the cotransformed recombination substrates were of complementary sequence, so that direct annealing was possible. Recombination was inefficient when both single-stranded recombination partners contained homologous regions of identical sequence and generation of a double-stranded DNA was required prior to heteroduplex formation. These results indicate that direct annealing of single strands is an important initial step for intermolecular recombination in tobacco cells. Annealed cotransformed single-stranded molecules yielded intermediates that could be further processed by either continuous or discontinuous second-strand synthesis. The type of intermediate had no influence on the recombination efficiency. Double-stranded circles were unable to recombine efficiently either with each other or with single-stranded DNA. Our results suggest that a helicase activity is involved in the initial steps of double-stranded DNA recombination which unwinds duplex molecules at the site of double-strand breaks.
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Maryon, E., and D. Carroll. "Characterization of recombination intermediates from DNA injected into Xenopus laevis oocytes: evidence for a nonconservative mechanism of homologous recombination." Molecular and Cellular Biology 11, no. 6 (June 1991): 3278–87. http://dx.doi.org/10.1128/mcb.11.6.3278.

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Homologous recombination between DNA molecules injected into Xenopus laevis oocyte nuclei is extremely efficient if injected molecules have overlapping homologous ends. Earlier work demonstrated that ends of linear molecules are degraded by a 5'----3' exonuclease activity, yielding 3' tails that participate in recombination. Here, we have characterized intermediates further advanced along the recombination pathway. The intermediates were identified by their unique electrophoretic and kinetic properties. Two-dimensional gel electrophoresis and hybridization with oligonucleotide probes showed that the intermediates had heteroduplex junctions within their homologous overlaps in which strands ending 3' were full length and those ending 5' were shortened. Additional characterization suggested that these intermediates had formed by the annealing of complementary 3' tails. Annealed junctions made in vitro were rapidly processed to products, indicating that they are on the normal recombination pathway. These results support a nonconservative, single-strand annealing mode of recombination. This recombination mechanism appears to be shared by many organisms, including bacteria, fungi, plants, and mammals.
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Maryon, E., and D. Carroll. "Characterization of recombination intermediates from DNA injected into Xenopus laevis oocytes: evidence for a nonconservative mechanism of homologous recombination." Molecular and Cellular Biology 11, no. 6 (June 1991): 3278–87. http://dx.doi.org/10.1128/mcb.11.6.3278-3287.1991.

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Homologous recombination between DNA molecules injected into Xenopus laevis oocyte nuclei is extremely efficient if injected molecules have overlapping homologous ends. Earlier work demonstrated that ends of linear molecules are degraded by a 5'----3' exonuclease activity, yielding 3' tails that participate in recombination. Here, we have characterized intermediates further advanced along the recombination pathway. The intermediates were identified by their unique electrophoretic and kinetic properties. Two-dimensional gel electrophoresis and hybridization with oligonucleotide probes showed that the intermediates had heteroduplex junctions within their homologous overlaps in which strands ending 3' were full length and those ending 5' were shortened. Additional characterization suggested that these intermediates had formed by the annealing of complementary 3' tails. Annealed junctions made in vitro were rapidly processed to products, indicating that they are on the normal recombination pathway. These results support a nonconservative, single-strand annealing mode of recombination. This recombination mechanism appears to be shared by many organisms, including bacteria, fungi, plants, and mammals.
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Rauscher, G. "Sixtus-Tonks experiments on strand annealed 49Co-2V-Fe wires." IEEE Transactions on Magnetics 21, no. 5 (September 1985): 1930–32. http://dx.doi.org/10.1109/tmag.1985.1064075.

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Dissertations / Theses on the topic "And strand annealers"

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G, C. Keshav. "Investigation of the Role of Bacterial Ribosomal RNA Methyltransferase Enzyme RsmC in Ribosome Biogenesis." Kent State University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=kent1621868567263046.

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Srour, Mohammed R. "X-ray Scattering Study of the Strain In Annealed Silica." Ohio University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1398343409.

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Greisert, Carsten [Verfasser]. "Strain-induced formation of martensite during forming and springback behaviour of annealed and hard cold-rolled stainless steel grades EN 1.4301 and EN 1.4318 / Carsten Greisert." Aachen : Shaker, 2004. http://d-nb.info/1170534848/34.

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Book chapters on the topic "And strand annealers"

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Fang, Xiao Ying, Xin Zhang, Hong Guo, Wei Guo Wang, and Bang Xin Zhou. "Grain Boundary Character Distributions of Strain-Annealed 304 Stainless Steel." In Advanced Materials Research, 1335–40. Stafa: Trans Tech Publications Ltd., 2007. http://dx.doi.org/10.4028/0-87849-463-4.1335.

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Bak-Misiuk, J. "X-ray Study of Strain Relaxation in Heteroepitaxial Layers of Semiconductors Annealed under High Hydrostatic Pressure." In Frontiers of High Pressure Research II: Application of High Pressure to Low-Dimensional Novel Electronic Materials, 263–74. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0520-3_20.

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Cedergren, Stefan, Joakim Johansson, and Göran Sjöberg. "Influence of Deformed Surface Layer When Machining Wrought Alloy 718 in an Annealed and in a Pre-Strained Condition." In 8th International Symposium on Superalloy 718 and Derivatives, 263–71. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2014. http://dx.doi.org/10.1002/9781119016854.ch22.

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Hanning, Fabian, Gurdit Singh, and Joel Andersson. "The Effect of Grain Size on the Susceptibility Towards Strain Age Cracking of Wrought Haynes® 282®." In Advances in Transdisciplinary Engineering. IOS Press, 2020. http://dx.doi.org/10.3233/atde200178.

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The effect of grain size on the suceptibility towards strain age cracking (SAC) has been investigated for Haynes® 282® in the tempeature range of 750 to 950∘C after isothermal exposure up to 1800s. Grain growth was induced by heat treating the material at 1150∘C for 2h, resulting in a fourfold increase in grain size. Hardness was significanlty reduced after heat treatment as compared to mill-annealed material. Large grain size resulted in intergranular fracture over a wider temperature range than small grain size material. Ductility was lowest at 850∘C, while lower values were observed to be correlated to increased grain size. The rapid formation of grain boundary carbide networks in Haynes® 282® is found to be not able to compensate for higher local stresses on grain boundaries due to incresed grain size.
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Hatanaka, Kenji, Takeshi Uchitani, and Satoshi Kawaraya. "A numerical calculation on cyclic stress-strain response in the vicinity of crack tip in annealed copper." In Low Cycle Fatigue and Elasto-Plastic Behaviour of Materials, 635–40. Elsevier, 1998. http://dx.doi.org/10.1016/b978-008043326-4/50106-9.

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Kohzuki, Yohichi. "Study on Influence of a State of Dopants on Dislocation-Dopant Ions Interaction in Annealed Crystals." In Alkaline Chemistry and Applications [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96395.

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Combination method of strain-rate cycling tests and application of ultrasonic oscillations was conducted for KCl:Sr2+ (0.035, 0.050, 0.065 mol.% in the melt) single crystals at low temperatures. The measurement of strain-rate sensitivity (λ) of flow stress under the application of ultrasonic oscillatory stress provides useful information on the interaction between a mobile dislocation and impurities (Sr2+ ions) during plastic deformation and the variation of λ with stress decrement (Δτ) due to oscillation has stair-like shape: The first plateau place ranges below the first bending point (τp1) at low stress decrement and the second one extends from the second bending point (τp2) at high stress decrement. The value of λ decreases with the Δτ between the two bending points. The τp1 is considered to represent the effective stress due to impurities when a dislocation begins to break-away from the impurities with the help of thermal activation during plastic deformation. Annealing the impure crystal by heat treatment, τp1 decreases obviously at low temperature and the critical temperature Tc, at which τp1 is zero, also becomes slightly smaller. Furthermore, it was investigated whether a change in the state of a small amount of impurities has an influential factor of the flow parameters (e.g., the activation energy, the density of forest dislocations) from the data analyzed in terms of Δτ vs. λ curve.
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Conference papers on the topic "And strand annealers"

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Emblom, William J., Md F. S. Ibne Islam, Richard J. Jones, Mitra Aithal, Scott Wagner, Thomas Pesacreta, and Gary A. Glass. "Comparing Methods for Establishing Multiscale Material Properties of 0.2 mm Thick Annealed ASTM 304." In ASME 2015 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/imece2015-50019.

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Producing fuel cells bipolar plates and other devices such as microscale heat exchangers for electronics requires both macroscale and microscale forming processes. At the macroscale, typically, mechanical properties of sheet metal are determined by performing tensile tests. In addition, it has long been recognized that bi-axial tension tests, dome tests, and hydroforming or viscous bulge tests provide the basis for improved understanding of the mechanics of sheet metal forming. At the microscale strain gauges are too large for measuring strains in small regions and membrane theory is only valid at the poles of the bulge. Continuum mechanics models are useful but require tedious thickness measurements for multiple work pieces, requiring extensive sample preparation and analysis. In this paper experimental results from hydroforming tests for 0.2-mm thick annealed ASTM 304 stainless steel sheet in 11 mm, 5 mm, and 1 mm diameter open dies at various pressures were evaluated. The height of the bulge at the pole and strains based upon measurements of 127 micron strain grids were determined. These dies represent the transition from a small macroscale process to a microscale forming process. Two methods were used to estimate material properties: an analytical model and an iterative method which compared experimental strain results with the strains from a finite element model where the Holloman constitutive properties of the sheet were varied. The problems estimating material properties based upon grid strain measurement, membrane theory, and the iterative finite element approaches were investigated and the results were compared. This study indicates that membrane theory will provide adequate predictions for Holloman constructive properties provided the assumptions for membrane theory are not violated. However, using measured microscale grid deformation strains does not produce very good agreement estimates of the Holloman constitutive model when comparing experimental results with FEA strains. It is believed that while the grid strain measurement method used results in strain measurement errors of less than 1.5% of strain, this error is sufficient to result in enough uncertainty to produce results that are inconsistent with other methods.
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Sunny, George P., Vikas Prakash, and John J. Lewandowski. "Effects of Annealing on Dynamic Behavior of a Bulk Metallic Glass." In ASME 2005 International Mechanical Engineering Congress and Exposition. ASMEDC, 2005. http://dx.doi.org/10.1115/imece2005-83016.

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Bulk metallic glasses are excellent candidate materials for integral armor because of their high strength and large elastic strain. Also, annealed glasses exhibit higher yield strengths than their fully amorphous counterparts. Therefore, the dynamic behavior of an annealed bulk metallic glass, Zr41.25Ti13.75Ni10Cu12.5Be22.5, was investigated in this study. A Split-Hopkinson Pressure Bar (SHPB) was employed to determine the stress-strain response at strain rates varying from 500/s-2000/s for the annealed glass. Also, a high-speed camera was utilized to obtain in-situ video of the specimen during the deformation process. These results were then compared to similar tests on the fully amorphous material. Finally, a new specimen design and experimental setup was proposed to promote accurate measurements from SHPB tests. During dynamic loading, the strain to failure increased as the strain-rate was increased, and the changes in strain-rate produced a larger effect than that of the annealing treatment on the failure strain. Maximum stresses were generally higher for the annealed glass, although there was some scatter. Finally, while an instability formed a dominant shear band for the as-received material, extensive fragmentation characterized the deformation after formation of an initial crack.
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Boninelli, S., and F. Cristiano. "Damage recovery and strain induced by Phosphorous in laser annealed Ge." In 2018 18th International Workshop on Junction Technology (IWJT). IEEE, 2018. http://dx.doi.org/10.1109/iwjt.2018.8330286.

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Li, Jingjing, S. Jack Hu, John E. Carsley, Theresa M. Lee, Louis G. Hector, and Sushil Mishra. "Post-Anneal Mechanical Properties of Pre-Strained AA5182-O Sheets." In ASME 2011 International Manufacturing Science and Engineering Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/msec2011-50142.

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The effects of different pre-strain levels, paths and subsequent annealing on the post-annealing mechanical properties of AA5182-O were investigated. Aluminum sheet specimens were pre-strained in uniaxial, plane strain and equibiaxial tension to several equivalent strain levels, annealed at 350°C for short (10 seconds) and long (20 minutes) durations, and then tested for post-annealing mechanical properties, including tensile properties, anisotropy and forming limits. The tensile properties, R-values at 0°, 45° and 90° relative to the sheet rolling direction, and forming limit diagrams (FLDs) exhibited dependencies of pre-strain and annealing history. The importance of the process variables and their effects were identified via designed experiments and analysis of variance. Three-dimensional digital image correlation, which captured the onset of local necking, was employed in the FLD development. Texture in the as-received and deformed sheets was investigated with electron backscattered diffraction and provided a means for linking prestrain and static recovery or recrystallization with microstructure. This guided the understanding of the mechanical property changes observed after preforming and annealing. Ultimately, the expanded forming limit curve demonstrated the advantage of annealing in extending the formability of strained AA5182-O.
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Miller, David A., and Cameron K. Chen. "Application of Advanced Constitutive Models to the Simulation of Machining." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-10842.

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Advanced constitutive models have long been used to describe plastic material response at high strains and high strain rates. These models include the Johnson-Cook, Zerrelli-Armstrong and Material Threshold Stress (MTS) formulations, each with a separate fidelity. The constitutive parameters for these complex models are commonly identified using laboratory techniques such as quasi-static load frames at room and elevated temperatures, Split Hopkinson Pressure Bars (SHPB) in tension and compression, gas guns, and Taylor impact cylinders. However, while the models are able to adequately describe material response under high strain and high strain rate, the loadings are all uniaxial in nature. The ability of these constitutive models and parameters to describe a different dynamic loading event, namely shear dominated machining, has not been thoroughly investigated. This work will develop numerical simulations applying multiple constitutive models with material parameters experimentally determined for fully annealed copper samples. Ultimately, the machining simulation will be compared with high fidelity experimental machining data. The utility of this research extends to the fundamental questions that surround the machining process, such as tool forces, surface damage, precision and quality.
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Sigamani, Nirmal Shankar, Saad Ahmed, and Zoubeida Ounaies. "Effect of Processing Conditions on the Microstructure and Electromechanical Response of PVDF TrFE CTFE Terpolymers." In ASME 2014 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/smasis2014-7696.

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Effect of annealing time on the micro structure and electromechanical properties PVDF TrFE CTFE terpolymer with 7.8 mol % CTFE was investigated to better understand structure-property relationships in these polymers. DSC results show that the 9 hr-annealing time gives the maximum enthalpy of melting which suggests the crystallinity of the polymer is increased. WAXS results show that annealing increases the proportion of the γ-phase with the ratio of the intensity of γ-phase to α-phase increases with more annealing time. Dielectric spectroscopic study of 9hr-annealed film also suggests that the film shows complete relaxor ferroelectric behavior with a maximum electrostrictive strain up to 4.4 % compared to 4.1 % for the 2.5 hr-annealed film. Finally, annealing time higher than 9 hrs result in a decrease in the electrostrictive strain.
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Kemper, Bart. "Application of Annealed Cable for Vehicle Arresting Barriers." In ASME 2002 International Mechanical Engineering Congress and Exposition. ASMEDC, 2002. http://dx.doi.org/10.1115/imece2002-32464.

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Transportation and traffic managers have had to deal with increased liability issues regarding containing vehicles during impact with protective barriers. Today’s security environment has a heightened need for means of stopping vehicles in controlled manner for security and liability purposes. Using strain energy absorption via annealed steel cables has proven to be a commercial success. Gated vehicle barrier applications using this mechanism range from safely stopping runaway vehicles at railroad crossing in compliance with National Cooperative Highway Research Program (NCHRP) Report 350 to stopping a potential attacker at an industrial plant in compliance with Nuclear Regulation (NUREG) CR-6190. This paper will examine different applications of this mechanism for controlling vehicle impact, analyze the nonlinear interactions at work, and develop operating parameters for using annealed steel wire rope for these applications.
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Uddin, Md Mosleh, Debabrata Mondal, and Paul D. Herrington. "Finite Element Simulation of Backward Micro Extrusion for Annealed Copper." In ASME 2018 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/imece2018-86755.

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The growing demand of miniaturized products is tremendously influencing the progress of micro-forming technologies. The implementations of micro technologies in the field of microelectronics, sensors, and medical equipment necessitate versatile micro-forming processes. These processes facilitate the bulk production of micro parts with higher precision, minimum material waste, and better surface finish. However, micro-forming technologies are still expensive due to the limitations of traditional materials and stringent size requirements. Finite element simulations are being widely used to analyze the manufacturing process parameters before going into production. In this research, a backward micro-extrusion process is simulated for annealed copper by using commercial Finite element simulation software. The effects of different punch diameters, friction coefficients, punch velocities on the load-displacement curves and the resulting strain distributions are investigated. To overcome limitations of the post-yield hardening data from the uniaxial compression test, the Ramberg-Osgood model is proposed to predict the responses at the higher plastic strain.
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Tsisar, Valentyn, Carsten Schroer, Olaf Wedemeyer, Aleksandr Skrypnik, and Jürgen Konys. "Effect of Structural State of Austenitic 15-15 Ti Steel on Initiation and Propagation of Solution-Based Corrosion Attack in Flowing Liquid Pb-Bi Eutectic at 400 and 500 °C." In 2017 25th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/icone25-66634.

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Investigations of corrosion behavior of austenitic 1.4970 (15–15 Ti) steel in Pb-Bi eutectic at 400–550 °C show an effect of structural state of material with identical composition on the depth of solution-based attack. Structural boundaries play a role of active paths along which the solution-based liquid-metal attack develops preferentially. In this view it is important to have quantitative and qualitative information about grain boundary type distribution in material and state of boundaries with respect to the accumulated strains. The EBSD analysis performed on 1.4970 steel in solution-annealed and cold-worked (40% reduction) states indicates that deformation increases substantially the total length of strained boundaries. The increase in fraction of active diffusion paths results in acceleration of corrosion attack on steel via solution.
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Sunny, George P., Vikas Prakash, and John P. Lewandowski. "Results From a Novel Insert Design for High Strain-Rate Compression of a Bulk Metallic Glass." In ASME 2006 International Mechanical Engineering Congress and Exposition. ASMEDC, 2006. http://dx.doi.org/10.1115/imece2006-15414.

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Abstract:
Liquidmetal-1 (LM-1, Zr41.25Ti13.75Cu12.5Ni10Be22.5) is a bulk metallic glass that can be processed in large thicknesses (e.g. 10 mm) because of its low critical cooling rate (e.g. 1 K/s). Like other bulk metallic glasses, this material exhibits near theoretical strength and large elastic strains (~2%) under quasi-static loading conditions. In this work, the Split-Hopkinson Pressure Bar (SHPB) was employed to perform high strain-rate compression tests on annealed LM-1. An ultrahigh-speed camera was also employed to perform in-situ video of the deformation process of the experiments, and the macroscopic fracture behavior was examined after testing. In addition, a new insert design was developed to reduce the effects of stress concentrations on the specimen. SHPB testing, combined with in-situ video, was performed on as-cast LM-1 using this new experimental configuration to determine the failure modes. The results of these experiments are compared to previous results to understand better the effects of stress concentration on high strain-rate behavior of bulk metallic glass.
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