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1

Barda, Myrto S., Tilemachos Chatzigeorgiou, George K. Papadopoulos, and Penelope J. Bebeli. "Agro-Morphological Evaluation of Lupinus mutabilis in Two Locations in Greece and Association with Insect Pollinators." Agriculture 11, no. 3 (March 11, 2021): 236. http://dx.doi.org/10.3390/agriculture11030236.

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Lupinus mutabilis Sweet is an Andean protein crop with agro-economic potential. However, it is characterized by low yields and phenotypic plasticity related to environmental conditions when cultivated in different locations in Europe. Current research objective was to evaluate L. mutabilis agro-morphological performance in two locations in Greece and to record its pollinators, since these can contribute to optimization of crop performance. For this purpose, eight Andean lupin accessions, one white and one blue lupin commercial varieties were evaluated for 71 agro-morphological traits in a Randomized Complete Block design with three replications. Combined Analysis over Location presented a significant accession-location interaction for traits of economic interest such as seed crude protein and 100 seed weight. Seed crude protein was higher in L. mutabilis accessions (up to 43.8 g 100 g−1 seed) than white and blue lupins. Andean lupin yielded up to 327 kg ha−1 (LIB214) in Kalamata, while its yield was lower than the white lupin in Athens. Using principal component analysis, three groups of accessions were formed, one by each lupin species and three within Andean lupin accessions. Floral traits such as color and size are related to pollinators diversity and preference. Insect pollinators and floral traits interplay among different lupin species was examined. Pollinators visited L. mutabilis flowers belong to the genera Anthophora, Bombus, Megachile and Xylocopa. The results of the present study, indicate that L.mutabilis has agronomic potential and enhances above ground biodiversity, however breeding for higher yields is required.
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2

Simioniuc, Danut Petru, Violeta Simioniuc, Denis Topa, Merlijn van den Berg, Udo Prins, Penelope J. Bebeli, and Iulian Gabur. "Assessment of Andean lupin (Lupinus mutabilis) Genotypes for Improved Frost Tolerance." Agriculture 11, no. 2 (February 13, 2021): 155. http://dx.doi.org/10.3390/agriculture11020155.

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Spring frost poses a challenge for all major crops and, in the case of Lupinus mutabilis (Andean lupin) can cause severe damage or even total loss of the crop. Within the LIBBIO project consortium, we conducted a series of experiments in order to develop a suitable protocol for screening lupin germplasm under frost-simulation conditions. Four lupin accessions, one Lupinus albus and three Andean lupins were used in the experiments (L. albus Mihai, L. mutabilis LIB 220, LIB 221, LIB 222). Seedlings at four developmental stages were challenged with five different levels of ‘frost’ stress from low (−2 °C) to high (−10 °C). Notably, young seedling (cotyledons just breaking through the soil surface) showed little evidence of frost damage for temperatures down to −6 °C. At −8 °C, however, damage was evident, suggesting a cold tolerance threshold occurs at this temperature. Interestingly, for later developmental stages, when the first and second leaves were visible, notable differences were observed starting at −6 °C. The results indicate that the plant growth stage is an important parameter when screening for frost tolerance in germplasm. Overall, by identifying Andean lupin genotypes adapted to high abiotic stress factors, farmers will be able to use it as a reference crop with potentially a commercial interest from the food sector, or cosmetics, and biofuel industries.
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3

Falconi, Cesar E., Richard G. F. Visser, and Sjaak van Heusden. "Influence of plant growth stage on resistance to anthracnose in Andean lupin (Lupinus mutabilis)." Crop and Pasture Science 66, no. 7 (2015): 729. http://dx.doi.org/10.1071/cp14104.

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Anthracnose, caused by Colletotrichum acutatum, is the most destructive fungal disease of Andean lupin (Lupinus mutabilis Sweet) in Ecuador and of other lupin species around the world. Symptoms of necrotic spots occur throughout the main stem, and infection progresses to cause bending of the main stem and lateral branches, resulting in yield loss. Although there is no known anthracnose resistance, this study aims to assess tolerance of Andean lupin and investigate lupin–C. acutatum interactions. Two Andean lupin genotypes, I-450 Andino and I-451 Guaranguito, were inoculated on the meristematic section of the main stem, either by spraying or by pipetting C. acutatum spores on to an artificial wound. Although the two methods gave similar results, spraying is the preferred method because it mimics natural pathogen infection. Plant-pathogen interactions were assessed at five different phenological stages (leaf stages 2–3, 4–5, 6–7, 8–9, and 10–11) with three C. acutatum isolates by using a 0–5 scale to assess disease symptoms. In both genotypes, anthracnose symptoms were greater at early seedling stage (2–3-leaf stage), decreasing significantly in early vegetative phase (6–7-leaf stage) and increasing again when the flower stage began (10–11-leaf stage). However, the tolerance of these two Andean lupin genotypes to anthracnose was not equally expressed at all developmental stages. We recommend, in a breeding program, that screening for anthracnose first occurs at the 6–7-leaf stage (6 weeks old) and again when flowering starts at the 10–11-leaf stage (10 weeks old) so that the overall tolerance can be determined. This method could be used in lupin breeding programs for improving resistance to anthracnose.
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4

Guilengue, Norberto, João Neves-Martins, and Pedro Talhinhas. "Response to Anthracnose in a Tarwi (Lupinus mutabilis) Collection Is Influenced by Anthocyanin Pigmentation." Plants 9, no. 5 (May 2, 2020): 583. http://dx.doi.org/10.3390/plants9050583.

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Anthracnose, caused by Colletotrichum lupini, is a major limiting factor for lupin production. Tarwi or Andean Lupin (Lupinus mutabilis) is generally regarded as susceptible to anthracnose, but the high protein and oil content of its seeds raise interest in promoting its cultivation in Europe. In this study we evaluated the response to anthracnose of 10 tarwi accessions contrasting in anthocyanin pigmentation, by comparison to white lupin (Lupinus albus), using a contemporary Portuguese fungal isolate. A severity rating scale was optimized, including weighted parameters considering the type of symptoms and organs affected. All tarwi accessions were classified as susceptible, exhibiting sporulating necroses on the main stem from seven days after inoculation. Anthracnose severity was lower on anthocyanin-rich tarwi plants, with accession LM34 standing out as the less susceptible. Accession I82 better combines anthracnose response and yield. In global terms, disease severity was lower on white lupin than on tarwi. Although based on a limited collection, the results of the study show the existence of genetic variability among L. mutabilis towards anthracnose response relatable with anthocyanin pigmentation, providing insights for more detailed and thorough characterization of tarwi resistance to anthracnose.
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5

Galek, R., E. Sawicka-Sienkiewicz, D. Zalewski, S. Stawiński, and K. Spychała. "Searching for low alkaloid forms in the Andean lupin (Lupinus mutabilis) collection." Czech Journal of Genetics and Plant Breeding 53, No. 2 (May 16, 2017): 55–62. http://dx.doi.org/10.17221/71/2016-cjgpb.

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The Andean lupin (Lupinus mutabilis Sweet) is the only cultivated species of the genus Lupinus originating from South America. Attempts were made to introduce this species to European soil and climatic conditions. The main disadvantages of the Andean lupin include too long and non-uniform maturation of pods in a plant, dropping flowers and pod buds, and a high alkaloid content (up to 5%). The aim of this study was to identify in post-mutagen treated material genotypes with low alkaloid content in seeds, which would be useful for improving L. mutabilis by breeding. The iodine test was annually performed to test the presence of alkaloids in seeds, using Lugol’s solution (I in KI). Based on the turbidity of the test samples, the investigated plants were divided into four groups, labelled as follows: G – individuals with a high content of alkaloids (very bitter), P – individuals with an intermediate content of alkaloids (bitter), PS – individuals with a low alkaloid content (fairly sweet) and S – individuals with a very low alkaloid level (sweet). For further propagation, mainly individuals from the S and PS groups were selected, and by part from the group P, while all bitter forms (G group) were rejected. Thirty chosen lines were examined for the content and composition of alkaloids by gas chromatography. The alkaloid content did not exceed 0.1% in 13 favourable lines, and even two lines had less than 0.05%. The seeds of the studied material contained 7 to 9 different alkaloids. The prevailing alkaloids in the seeds of the Andean lupin were 4-hydroxylupanine and lupanine. Significant progress has been achieved in reducing the content of alkaloids in subsequent generations of the investigated representatives of Lupinus mutabilis from South America.
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6

Bebeli, Penelope J., Efstathia Lazaridi, Tilemachos Chatzigeorgiou, Maria-José Suso, Waltraud Hein, Alexios A. Alexopoulos, Gonçalo Canha, et al. "State and Progress of Andean Lupin Cultivation in Europe: A Review." Agronomy 10, no. 7 (July 18, 2020): 1038. http://dx.doi.org/10.3390/agronomy10071038.

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Lupinus mutabilis is an important source of protein in different Andean countries, and its use in diets, particularly those of less wealthy individuals, has been observed for thousands of years. There is an increasing demand for protein crops suitable for Europe and this species is a potential candidate. Assessment of Lupinus mutabilis genetic material in European conditions started more than 40 years ago, with the characterization of a vast number of accessions from the Andean region. In this review, abiotic and biotic constraints to L. mutabilis cultivation in European soil and climatic conditions are discussed, and cultivation management practices are suggested. The beneficial interaction of L. mutabilis with Bradyrhizobium strains in the soil and various pollinator species is also discussed, and the effect of abiotic stresses on these interactions is highlighted. Prospects of alternative uses of L. mutabilis biomass in Northern Europe and opportunities for breeding strategies are discussed. In conclusion, the different approach to crop modeling for Southern and Northern European climatic conditions is highlighted.
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7

Lizarazo, Clara, Frederick Stoddard, Pirjo Mäkelä, and Arja Santanen. "Genetic variability in the physiological responses of Andean lupin to drought stress." Suomen Maataloustieteellisen Seuran Tiedote, no. 26 (January 31, 2010): 1–5. http://dx.doi.org/10.33354/smst.76862.

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Drought is one of the most important abiotic stresses that causes significant reductions in crop yield, and thushinders the food security of the growing world population. In consequence, it is urgent to select crops able toresist drought, maintain high yield and have a good nutritive content. The purpose of this project was toevaluate the responses of different accessions of Andean lupin to drought stress, and identify if there aresignificant differences in their physiological responses.To identify germplasm for further investigation, thirty accessions of Lupinus mutabilis Sweet. and oneaccession of L. albococcineus Hort. were screened in two sets, A of 15 and B of 16 accessions. From thesesets, four lines were chosen on the basis of extreme values in the measurements, and this set of four wasinvestigated in depth (set C). Water stress consisted of controlled water loss from the soil (at 2% per day).The screening techniques used were: leaf temperature, stomatal conductance, relative water content, waterpotential, ion membrane leakage, and shoot dry weight were measured and transpiration efficiency wascalculated. In addition, carbon isotope discrimination, root length, root dry matter, proline content andsoluble sugar content were included in only set C.Based on the results from set A and set B, accessions PI 457981, PI 457972, and AC 2792 were consideredas being drought resistant and PI 510572 was considered as drought sensitive. However, the results from setC showed that accession PI 510572 is able to adapt its cell membrane to the drought stress so that its ionleakage is less than in the other accessions, and in addition it has the ability to accumulate higherconcentrations of osmoprotectants.Thus, this survey identified that some accessions of Andean lupin were able to avoid drought stress throughstomatal traits and root traits, and others that were able to tolerate drought through the accumulation ofosmotically active substances. Consequently, there are good prospects for breeding of Andean lupin toimprove its drought resistance.
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8

Falconí, César E., and Viviana Yánez–Mendizábal. "Dry heat treatment of Andean lupin seed to reduce anthracnose infection." Crop Protection 89 (November 2016): 178–83. http://dx.doi.org/10.1016/j.cropro.2016.07.021.

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9

Lazaridi, Efstathia, George K. Papadopoulos, and Penelope J. Bebeli. "Andean Lupin Phenology and Agronomic Performance under Different Planting Dates in a Mediterranean Climate." Agronomy 10, no. 12 (December 21, 2020): 2020. http://dx.doi.org/10.3390/agronomy10122020.

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Andean lupin in Europe has regained interest in recent years due to its high seed protein and oil content and its potential use for food, feed as well as a crop used in biorefineries. A search for suitable germplasm in combination with a determination of appropriate agronomical practices such as planting date are needed for commercial farming in new areas. The current study aims to evaluate the performance of six selected L. mutabilis accessions in a Mediterranean area using two different planting dates for two consecutive experimental years. A split plot design with accessions as subplots and planting dates as main plots was used. Measurements such as days to flowering, plant height, total number of pods and seeds per plant, seed yield and seed crude protein content were taken. All accessions performed better when cultivation started not later than the end of autumn since high temperatures occurred during late spring shortened the growth cycle and reduced yield. Earlier cultivation applied, in the middle of autumn, did not enhance significantly seed yield production. Among the accessions, LIB223 was characterized by the highest seed crude protein content in the early planting treatment, while accessions with prolonged growth cycle (LIB209, LIB214 and LIB223) produced higher seed yield than other accessions and could be further studied as promising breeding material for cultivation under the edaphoclimatic conditions tested.
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10

Miano, Alberto Claudio, Jorge Armando García, and Pedro Esteves Duarte Augusto. "Correlation between morphology, hydration kinetics and mathematical models on Andean lupin (Lupinus mutabilis Sweet) grains." LWT - Food Science and Technology 61, no. 2 (May 2015): 290–98. http://dx.doi.org/10.1016/j.lwt.2014.12.032.

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11

Hardy, A., C. Huyghe, and J. Papineau. "Dry matter accumulation and partitioning, and seed yield in indeterminate Andean lupin (Lupinus mutabilis Sweet)." Australian Journal of Agricultural Research 48, no. 1 (1997): 91. http://dx.doi.org/10.1071/a96015.

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Despite the high oil and protein content of the seeds, the agronomic potential of Lupinus mutabilis Sweet, a South American lupin species, is limited because of its low seed yield and its uncertain maturity. Dry matter accumulation and partitioning, and seed yield, of 2 genotypes (early-maturing LM34 and late-maturing LM268) were studied in 2 seasons at 2 densities at Lusignan, France. Total dry matter at harvest was, on average, 680 g/m2. The mainstem and first-order branches mainly contributed to total plant biomass but pods were only produced on the mainstem. On average, across the different crops, the maximum leaf area index (LAI) was 2· 8. The duration of LAI above 2·5 was short and not correlated with the total dry matter production. The partitioning of the dry matter varied according to the genotype. LM34 showed enhanced pod growth, its harvest index (HI) was 0·32, and seed yield averaged 1·28 t/ha. LM268 showed enhanced vegetative growth, both HI and seed yield were lower (0·16 and 1·13 t/ha, respectively), and whole plant maturity was never reached. For both genotypes during the pod filling, no remobilisation of assimilates occurred from stems to pods.
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12

Salmanowicz, B. P. "Primary structure and polymorphism of 2S albumins from seeds of Andean lupin ( Lupinus mutabilis Sweet)." European Food Research and Technology 209, no. 6 (October 1, 1999): 416–22. http://dx.doi.org/10.1007/s002170050519.

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13

Berru, Luis Briceño, Patricia Glorio-Paulet, Clara Basso, Alessio Scarafoni, Felix Camarena, Alyssa Hidalgo, and Andrea Brandolini. "Chemical Composition, Tocopherol and Carotenoid Content of Seeds from Different Andean Lupin (Lupinus mutabilis) Ecotypes." Plant Foods for Human Nutrition 76, no. 1 (February 1, 2021): 98–104. http://dx.doi.org/10.1007/s11130-021-00880-0.

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14

Repo-Carrasco-Valencia, Ritva. "Nutritional Value and Bioactive Compounds in Andean Ancient Grains." Proceedings 53, no. 1 (August 3, 2020): 1. http://dx.doi.org/10.3390/proceedings2020053001.

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Quinoa (Cheopodium quinoa), kañiwa (Cheopodium pallidicaule), kiwicha (Amaranthus caudatus) and tarwi (Lupinus mutabilis) are ancient crops from the Andean region of South America. Recently, interest in these crops has grown, and worldwide demand for them has increased considerably. The aim of this study was to study the bioactive compounds and nutritional compositions of different varieties/ecotypes of quinoa, kañiwa, kiwicha and tarwi. Proximate, mineral, dietary fibre, fatty acid and amino acid compositions were evaluated. The content of phenolic compounds, tocopherols and phytosterols, and the folic acid and antioxidant capacity, were determined as well. The protein content of the grains was between 13.00% and 20.00%. More important than protein quantity is protein quality, which is demonstrated by the composition of the amino acids. All analysed grains, and especially the kañiwa, had very high lysine content. This amino acid is especially important in vegetarian diets because it is the limiting amino acid in cereal protein. The content of the total phenolic compounds in the studied grains was 27–58 mg gallic acid/100 g of sample. In quinoa, the principal flavonoids found were quercetin and kaempferol, in kañiwa quercetin and isorhamnetin. In kiwicha, no detectable amounts of flavonoids were found. Plant sterols (phytosterols) were another group of biologically active compounds detected. Andean lupin, tarwi, is very rich in oil, which has a beneficial nutritional composition. In conclusion, all studied grains have a very high nutritional value, are interesting sources of bioactive compounds and could be used as ingredients in health-promoting functional foods.
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Falconí, C. E., and V. Yánez-Mendizábal. "Efficacy of UV-C radiation to reduce seedborne anthracnose (Colletotrichum acutatum ) from Andean lupin (Lupinus mutabilis )." Plant Pathology 67, no. 4 (December 18, 2017): 831–38. http://dx.doi.org/10.1111/ppa.12793.

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16

Córdova‐Ramos, Javier S., Patricia Glorio‐Paulet, Felix Camarena, Andrea Brandolini, and Alyssa Hidalgo. "Andean lupin ( Lupinus mutabilis Sweet): Processing effects on chemical composition, heat damage, and in vitro protein digestibility." Cereal Chemistry 97, no. 4 (June 17, 2020): 827–35. http://dx.doi.org/10.1002/cche.10303.

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17

Muñoz, Erika B., Diego A. Luna-Vital, Marco Fornasini, Manuel E. Baldeón, and Elvira Gonzalez de Mejia. "Gamma-conglutin peptides from Andean lupin legume (Lupinus mutabilis Sweet) enhanced glucose uptake and reduced gluconeogenesis in vitro." Journal of Functional Foods 45 (June 2018): 339–47. http://dx.doi.org/10.1016/j.jff.2018.04.021.

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18

Gross, R., E. von Baer, F. Koch, R. Marquard, L. Trugo, and M. Wink. "Chemical composition of a new variety of the Andean lupin (Lupinus mutabilis cv. Inti) with low-alkaloid content." Journal of Food Composition and Analysis 1, no. 4 (November 1988): 353–61. http://dx.doi.org/10.1016/0889-1575(88)90035-x.

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19

Callohuari, Y., C. Vergara, and J. Jiménez. "Insect pests associated with Andean lupin (Lupinus mutabilis Sweet) and their parasitoids in Peruvian central coast – (Lima, La Molina)." Peruvian Journal of Agronomy 2, no. 2 (September 10, 2018): 27. http://dx.doi.org/10.21704/pja.v2i2.1199.

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20

Yánez-Mendizábal, Viviana, and César E. Falconí. "Efficacy of Bacillus spp. to biocontrol of anthracnose and enhance plant growth on Andean lupin seeds by lipopeptide production." Biological Control 122 (July 2018): 67–75. http://dx.doi.org/10.1016/j.biocontrol.2018.04.004.

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21

Hardy, A., C. Huyghe, M. A. Rahim, P. Roemer, J. M. Neves-Martins, E. Sawicka-Sienkiewicz, and P. D. S. Caligari. "Effects of genotype and environment on architecture and flowering time of indeterminate Andean lupins (Lupinus mutabilis Sweet)." Australian Journal of Agricultural Research 49, no. 8 (1998): 1241. http://dx.doi.org/10.1071/a98060.

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Plant structure was shown to limit the seed yield of Lupinus mutabilis through low dry matter production. The genetic and environmental variations of architectural and phenological traits of indeterminate Andean lupin were evaluated. Twelve genotypes were sown at 6–8 dates at 5 locations in Europe in 1994 and 1995. Wide variation was observed for phenological and architectural characters. The variation in the number of mainstem leaves was partly explained by the temperature. The mainstem height and flowering date were related to the number of mainstem leaves but flowering time was also affected independently by the enviromental conditions. The mainstem structure determined the potential number of first-order branches through the number of axillary buds. The actual number of branches was also determined by the prevailing environmental conditions during branch growth. The number of leaves on the second first-order branch was less susceptible to the environmental conditions than the number of leaves on the mainstem. Heritabilities were high for all characters except for the number of leaves on the second first-order branch, and the interactions between genotype and environmental condition were low. Breeding for architecture modification would thus be possible in L. mutabilis.
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22

Molina-Poveda, César, Mariela Lucas, and Miguel Jover. "Evaluation of the potential of Andean lupin meal (Lupinus mutabilis Sweet) as an alternative to fish meal in juvenile Litopenaeus vannamei diets." Aquaculture 410-411 (October 2013): 148–56. http://dx.doi.org/10.1016/j.aquaculture.2013.06.007.

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23

Rybiński, Wojciech, Robert Rusinek, Bogusław Szot, Jan Bocianowski, and Michał Starzycki. "Analysis of interspecies physicochemical variation of grain legume seeds." International Agrophysics 28, no. 4 (October 1, 2014): 491–500. http://dx.doi.org/10.2478/intag-2014-0039.

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Abstract The paper presents an attempt to assess the reaction of seeds to mechanical loads taking into account their geometry expressed as seed thickness and 1000 seed weight. The initial material comprised 33 genotypes of grain legume plants and included cultivars registered in the country and breeding lines that are subject to pre-registration trials. The analysis of variance revealed significant diversity of the cultivars and lines of the species studied in terms of each of the analysed trait. The highest weight of 1000 seeds were obtained for white lupine seeds and peas, the lowest for andean lupine seeds. The maximum deformation and energy were obtained for white lupine seeds, the lowest for pea seeds, the maximum force and module the lowest values were determined for narrow-leafed lupine and pea. The highest values of protein were obtained for andean and yellow lupine, a fat content for andean and white lupine. The fatty acid profile as much as 70% or more were linoleic and oleic acids. Against the background of all the species are distinguished by white lupine seeds with a high content of oleic acid and the lowest of linoleic acid, for yellow lupine were obtained the inverse ratio of the two acids.
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Falconí, César E., Richard G. F. Visser, and Adriaan W. van Heusden. "Phenotypic, Molecular, and Pathological Characterization of Colletotrichum acutatum Associated with Andean Lupine and Tamarillo in the Ecuadorian Andes." Plant Disease 97, no. 6 (June 2013): 819–27. http://dx.doi.org/10.1094/pdis-02-12-0175-re.

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Anthracnose is a serious problem of both Andean lupine and tamarillo in Ecuador. Morphological features, internal transcribed spacer (ITS) sequences, and host specificity were used to characterize Colletotrichum isolates from lupine and tamarillo. Based on phenotypic and molecular characterization, the causal agent of anthracnose on both hosts was Colletotrichum acutatum. All isolates were identified in a C. acutatum-specific polymerase chain reaction assay. Colony diameter, conidia shape, and insensitivity to benomyl also placed isolates from both hosts in the C. acutatum group. However, a detailed analysis of the ITS sequences placed the lupine and tamarillo isolates from the Ecuadorian Andean zone in two clades, with both lupine and tamarillo isolates in each clade. C. acutatum isolates from Andean lupine were distinct from other C. acutatum isolates on lupine around the world. In cross-infection studies, the diameter of lesions produced by isolates from each host was compared on the main stem of two tamarillo and three lupine cultivars. Some isolates produced larger lesions on the host from which they were isolated but others showed similar aggressiveness on their alternate host. Isolates from both hosts were biotrophic on lupine stems, producing little necrosis and abundant sporulation whereas, on tamarillo stems, they produced dark lesions with few conidia. The collection of C. acutatum isolates from lupine and tamarillo provides interesting material for the study quantitative host adaptation.
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Mariotti, François, Maria E. Pueyo, Daniel Tomé, and Sylvain Mahé. "The bioavailability and postprandial utilisation of sweet lupin (Lupinus albus)-flour protein is similar to that of purified soyabean protein in human subjects: a study using intrinsically15N-labelled proteins." British Journal of Nutrition 87, no. 4 (April 2002): 315–23. http://dx.doi.org/10.1079/bjn2002526.

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Sweet lupin (Lupinus albus), a protein-rich legume devoid of anti-nutritional factors, is considered to have a high potential for protein nutrition in man. Results concerning the nutritional value of lupin protein are, however, conflicting in animals and very scarce in human subjects. Furthermore, where fibre-rich protein sources are concerned, the long-term nutritional results are often obscured, particularly since fibre-promoted colonic fermentation may bias the energy supply and redistribute N flux. We therefore studied, during the postprandial phase, the bioavailability and utilisation of lupin-flour protein in nine healthy men who had ingested a mixed meal containing intrinsically15N-labelled lupin flour as the protein source (Expt 1). The real ileal digestibility (RID) and ileal endogenous N losses (IENL) were assessed using a perfusion technique at the terminal ileum, and the N content and15N enrichment of ileal samples. Lupin flour exhibited a high RID of 91 (SD 3) % AND LOW IENL (5·4 (sd 1·3) mmol N/h). Postprandial dietary deamination was also assessed from body dietary urea and urinary dietary N excretion, and compared with results in nine healthy men following an isoenergetic meal containing a15N-soyabean-protein isolate with a similar RID, as a control (Expt 2). Postprandial dietary deamination was similar after lupin and soyabean meals (17 (sd 2) and 18 (sd 4) % ingested N respectively). We therefore conclude that lupin protein is highly bioavailable, even if included in fibre-rich flour, and that it can be used with the same efficiency as soyabean protein to achieve postprandial protein gain in healthy human subjects.
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Lin, Haibo, Eiichi Nakai, and Dachun Yang. "Boundedness of Lusin-area andgλ*functions on localized Morrey-Campanato spaces over doubling metric measure spaces." Journal of Function Spaces and Applications 9, no. 3 (2011): 245–82. http://dx.doi.org/10.1155/2011/187597.

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Letχbe a doubling metric measure space andρan admissible function onχ. In this paper, the authors establish some equivalent characterizations for the localized Morrey-Campanato spacesερα,p(χ)and Morrey-Campanato-BLO spacesε̃ρα,p(χ)whenα∈(-∞,0)andp∈[1,∞). Ifχhas the volume regularity Property(P), the authors then establish the boundedness of the Lusin-area function, which is defined via kernels modeled on the semigroup generated by the Schrödinger operator, fromερa,p(χ)toε̃ρa,p(χ)without invoking any regularity of considered kernels. The same is true for thegλ*function and, unlike the Lusin-area function, in this case,χis even not necessary to have Property(P). These results are also new even forℝdwith thed-dimensional Lebesgue measure and have a wide applications.
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Córdova-Ramos, Javier S., Patricia Glorio-Paulet, Alyssa Hidalgo, and Felix Camarena. "Effect of technological process on antioxidant capacity and total phenolic content of Andean lupine (Lupinus mutabilis Sweet)." Scientia Agropecuaria 11, no. 2 (June 8, 2020): 157–65. http://dx.doi.org/10.17268/sci.agropecu.2020.02.02.

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Darío Zamora Zamora, Hernán, Ángel María Zamora-Burbano, Leandro Henrique Ribeiro Varão, Thiago Alves Lopes Silva, Jesus David Coral Medina, and Daniel Pasquini. "Potential of Andean lupine (Lupinus mutabilis) for biodiesel production via methanolic route: A review." Revista Virtual de Química 12, no. 4 (2020): 938–48. http://dx.doi.org/10.21577/1984-6835.20200068.

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29

Schoeneberger, H., S. Mor�n, and R. Gross. "Safety evaluation of water debittered andean lupins (Lupinus mutabilis): 12-week rat feeding study." Qualitas Plantarum Plant Foods for Human Nutrition 37, no. 2 (1987): 169–82. http://dx.doi.org/10.1007/bf01092053.

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Borek, Sławomir, Szymon Kubala, and Sylwia Kubala. "Regulation by sucrose of storage compounds breakdown in germinating seeds of yellow lupine (Lupinus luteus L.), white lupine (Lupinus albus L.) and Andean lupine (Lupinus mutabilis Sweet): I. Mobilization of storage protein." Acta Physiologiae Plantarum 34, no. 2 (November 3, 2011): 701–11. http://dx.doi.org/10.1007/s11738-011-0870-1.

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Borek, Sławomir, Stanisława Pukacka, and Krzysztof Michalski. "Regulation by sucrose of storage compounds breakdown in germinating seeds of yellow lupine (Lupinus luteus L.), white lupine (Lupinus albus L.) and Andean lupine (Lupinus mutabilis Sweet). II. Mobilization of storage lipid." Acta Physiologiae Plantarum 34, no. 3 (December 18, 2011): 1199–206. http://dx.doi.org/10.1007/s11738-011-0916-4.

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Gomez-Puerta, Luis A., Johan Carrasco, Maria T. Lopez-Urbina, and Armando E. Gonzalez. "Morphological and molecular identification of Spirocerca lupi (Nematoda: Spiruridae) found in the Andean fox (Lycalopex culpaeus)." Journal of Parasitic Diseases 42, no. 3 (May 24, 2018): 449–54. http://dx.doi.org/10.1007/s12639-018-1009-8.

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33

Mikić, A., B. Ćupina, V. Mihailović, Đ. Krstić, S. Antanasović, L. Zorić, V. Đorđević, V. Perić, and M. Srebrić. "Intercropping white (Lupinus albus) and Andean (Lupinus mutabilis) lupins with other annual cool season legumes for forage production." South African Journal of Botany 89 (November 2013): 296–300. http://dx.doi.org/10.1016/j.sajb.2013.06.015.

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34

Pascual-Chagman, Gloria, Juan Santa-Cruz-Olivos, Alyssa Hidalgo, Fernando Benavente, M. Carmen Pérez-Camino, Alejandrina Sotelo-Mendez, Luz María Paucar-Menacho, and Christian R. Encina-Zelada. "Lupinus mutabilisoil obtained by expeller press: Yield, physicochemical characterization, antioxidant capacity, fatty acids and oxidative stability analyses." Scientia Agropecuaria 12, no. 2 (June 1, 2021): 219–27. http://dx.doi.org/10.17268/sci.agropecu.2021.025.

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The oil of debittered and dehulled grains from two Andean lupins (Lupinus mutabilis) from Peru, Andenes and Común,was extracted by expeller press. The extraction yield and the proximal composition of both varieties were assessed. The oil and the cake, as well as the defatted flour from the variety with the highest extraction yield, werecharacterised for physico-chemical properties, total polyphenol content and antioxidant capacity. The oil shelf-life at 25 °C was extrapolated by Rancimat test. Andenes presented an oil yield of 22.1% (vs. 18.4% of Común),CIELAB colour coordinates of L* = 36.5, a* = 2.2, b* = 20, refractive index and density at 25ºC of 1.469 and 0.903 g/mL,acid number of 3.2 mg KOH/g, free fatty acids content of 1.6%, peroxide number of 2.7 meqO2/kg, p-anisidine number of 1.3, unsaponificable content of 58%, total antioxidant capacity 21.4 μmol TE/g and total polyphenol content of 7.0 mg AGE/100 g. The predominant fatty acid, tocopherol and phytosterol in the oil were oleic acid (56.2%), γ-tocopherol (555 mg/kg) and β-sitosterol (41900 mg/100 g), respectively. The oil shelf-life at 25 ºC was 2.7 years. The Lupinus mutabilis oil extracted by expeller presented an acceptable quality with a lower environmental negative impact than the oils obtained by solvent extraction.
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35

Alkemade, J. A., M. M. Messmer, R. T. Voegele, M. R. Finckh, and P. Hohmann. "Genetic diversity of Colletotrichum lupini and its virulence on white and Andean lupin." Scientific Reports 11, no. 1 (June 29, 2021). http://dx.doi.org/10.1038/s41598-021-92953-y.

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AbstractLupin cultivation worldwide is threatened by anthracnose, a destructive disease caused by the seed- and air-borne fungal pathogen Colletotrichum lupini. In this study we explored the intraspecific diversity of 39 C. lupini isolates collected from different lupin cultivating regions around the world, and representative isolates were screened for their pathogenicity and virulence on white and Andean lupin. Multi-locus phylogeny and morphological characterizations showed intraspecific diversity to be greater than previously shown, distinguishing a total of six genetic groups and ten distinct morphotypes. Highest diversity was found across South America, indicating it as the center of origin of C. lupini. The isolates that correspond to the current pandemic belong to a genetic and morphological uniform group, were globally widespread, and showed high virulence on tested white and Andean lupin accessions. Isolates belonging to the other five genetic groups were mostly found locally and showed distinct virulence patterns. Two highly virulent strains were shown to overcome resistance of advanced white lupin breeding material. This stresses the need to be careful with international seed transports in order to prevent spread of currently confined but potentially highly virulent strains. This study improves our understanding of the diversity, phylogeography and pathogenicity of a member of one of the world’s top 10 plant pathogen genera, providing valuable information for breeding programs and future disease management.
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Hurej, Michał, Halina Kucharczyk, Jacek P. Twardowski, and Andrzej Kotecki. "Thrips (Thysanoptera) associated with two morphological forms of Andean lupin (Lupinus mutabilis)." Biologia 70, no. 7 (January 1, 2015). http://dx.doi.org/10.1515/biolog-2015-0106.

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AbstractThe aim of this study was to determine thrips abundance, species composition, sex ratio and seasonal dynamics of these insects on two morphological forms of Andean lupin (epigonal and semiepigonal). Thrips were studied in the lupine plants sown at two dates at the Experimental Research Station at Pawłowice, near Wrocław, Lower Silesia, Poland, during two growing seasons (2011-2012). The experiment was designed as a split-plot with four replicates for each morphological form and time of sowing. For the determination of thrips species’ composition, plants were collected from the central part of each plot when lupin was at the full blooming stage. In the laboratory, plants were shaken over a sheet of white paper. Once in each season thrips were also collected with a sweep net. In the laboratory larvae and adults were identified to species level and separated into males and females. During the whole lupin growing season, insects were also counted weekly on 10 consecutive plants in the middle row of each plot. Frankliniella intonsa, Thrips major and Aeolothrips intermedius were the dominant species in the full blooming stage of both semiepigonal and epigonal forms of Andean lupin. Thrips tabaci also occurred in greater numbers. There were no significant differences between numbers of thrips and number of species occurring on semiepigonal and epigonal plants sown at the same time. In the most numerous species, like F. intonsa and T. major, mainly females occurred in their population. In the population of A. intermedius males were abundant. Thrips occurring in Lupinus mutabilis, irrespective of the used form of lupin or sowing time, had one peak of abundance, i.e., the last ten days of June and the first ten days of July, when plants were at the blooming stage.
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Miano, Alberto C., Meliza L. Rojas, and Pedro E. D. Augusto. "Using ultrasound for improving hydration and debittering of Andean lupin grains." Journal of Food Process Engineering 42, no. 6 (June 26, 2019). http://dx.doi.org/10.1111/jfpe.13170.

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38

Chirinos-Aria, Michelle C., and Jorge E. Jiménez. "Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet)." Scientia Agropecuaria, March 20, 2015, 51–58. http://dx.doi.org/10.17268/sci.agropecu.2015.01.05.

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39

Yánez-Mendizábal, Viviana, and César E. Falconí. "Bacillus subtilis CtpxS2-1 induces systemic resistance against anthracnose in Andean lupin by lipopeptide production." Biotechnology Letters, January 3, 2021. http://dx.doi.org/10.1007/s10529-020-03066-x.

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40

Chirinos-Arias, Michelle C., Jorge E. Jiménez, and Lizbeth S. Vilca-Machaca. "Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers." Scientia Agropecuaria, March 30, 2015, 17–30. http://dx.doi.org/10.17268/sci.agropecu.2015.01.02.

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41

Struelens, Quentin, Diego Mina, and Olivier Dangles. "Combined effects of landscape composition and pesticide use on herbivore and pollinator functions in smallholder farms." CABI Agriculture and Bioscience 2, no. 1 (February 17, 2021). http://dx.doi.org/10.1186/s43170-021-00027-w.

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Abstract Background Landscape composition has the potential to foster regulating ecosystem services such as pollination and biocontrol in temperate regions. However, most landscape studies do not take pesticide use into account even though it is the main control strategy worldwide and has negative impacts on beneficial insects. Moreover, few studies have explored these combined effects in smallholder cropping system with diverse landscapes and small cultivated fields. Methods We assessed the effect of semi-natural cover and pesticide use on pollinator and herbivore abundances and functions in 9 fields in the Ecuadorian Andes through participatory experiments with smallholder farmers. We performed a path analysis to quantify the effects of landscape and pesticide use on herbivory, pollination and ultimately yield. Results Pesticide use significantly reduced pollinator abundance but had no significant effect on pest abundance. Similarly, we found non-significant effects of landscape composition on either herbivory and pollination. The study also provides new information on understudied Andean lupine’s pests and pollinators, whose application for small farmers is discussed. Finally, we hypothesize that peculiarities of tropical smallholder cropping systems and landscapes could explain the non-significant landscape effects on insect-based processes, which calls for more research in places outside the well-studied temperate region. Conclusions Landscape composition did not show any significant effect on pest and pollinator while pesticide use decreased the abundance pollinators, but with no significant effect on yield. This study also provides information about Andean lupine reproduction and overcompensation mechanisms that could be of interest for local farmers and researchers of this understudied crop.
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