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Journal articles on the topic 'Anti-bacterial assay'

Author: Grafiati

Published: 1 June 2024

Last updated: 31 July 2025

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1

Pradheeba, M., M. Pugalenthi, M. A. Deepa, S. Vishnu Kumar, and G. Vasukipridharshini. "Evaluation of Phytochemical Profile and In Vitro Antioxidant, Anti-bacterial and Anti-inflammatory activity of Piper schmidtii Hook. fil. A Wild Edible Fruit." Asian Pacific Journal of Health Sciences 9, no. 3 (2022): 191–97. http://dx.doi.org/10.21276/apjhs.2022.9.3.39.

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Aim: The present study aims at screening the phytochemical components and evaluates the antioxidant, anti-bacterial, and anti-inflammatory activity of fruit of Piper schmidtii, an endemic plant species from The Nilgiris, Tamil Nadu. Materials and Method: The different polar solvents such as petroleum ether, chloroform, ethyl acetate, methanol, and water were used and extraction was carried out using the soxhlet apparatus. The extracts were screened for qualitative and quantitative phytochemical analysis. The extracts of P. schmidtii were also subjected to in vitro-antioxidant activity by DPPH

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2

Wagh, Harshada. "Anti-oxidant and Anti-bacterial activity of polyherbal formulation." International journal of therapeutic innovation 2, no. 3 (2024): 0159–62. http://dx.doi.org/10.55522/ijti.v2i3.0051.

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The present work aimed to evaluate anti-oxidant and anti-bacterial activity of polyherbal formulation (gel) prepared using Tulsi, Aloe vera, Neem. It has been used for skin benefits since ancient times. Formulations were tested anti-oxidant activity by DPPH radical scavenging assay and anti-bacterial activity by disk diffusion Susceptibility Testing method. The anti-oxidant activity is exhibited in percentage inhibition. F1 formulation showed maximum percentage inhibition with increasing concentration as compared to standard ascorbic acid. The anti-bacterial activity is exhibited in zone of in

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Chakraborty, Rajdeep, Charbel Darido, Honghua Hu, Karen Vickery, and Shoba Ranganathan. "A novel drug combination strategy that redesigns the pharmacological management of oral cancer." Journal of Clinical Oncology 40, no. 16_suppl (2022): e18053-e18053. http://dx.doi.org/10.1200/jco.2022.40.16_suppl.e18053.

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e18053 Background: Biofilm formation is a continuous process in oral cancer patients, despite proper extirpation/elimination of a bacterial plaque via a surgical procedure or antibiotic treatment. Also, elimination of a bacterial plaque does not necessarily remove extant bacterial antigen-stimulated oral cancer cells. Therefore, combination drug treatment may be an appropriate approach to elucidate the confounding effects of bacterial antigens on anti-cancer drugs. Methods: Our drug combination strategy addressed both Gram-positive (Lipoteichoic acid [LTA]) and Gram-negative (Lipopolysaccharid

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Nofiani, Risa, Rizky Rizky, and Ridho Brilliantoro. "Antibacterial Activities and Toxicity of Streptosporangium sp. SM1P." Molekul 16, no. 3 (2021): 210. http://dx.doi.org/10.20884/1.jm.2021.16.3.780.

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This study aims to explore the anti-bacterial and toxicity activities from a rare actinobacterium isolated from mangrove, Mempawah District, West Kalimantan. The mangrove mud sample from Mempawah district was inoculated on ISP4 agar using a pour plate method. After 4 days of incubation, a colony of suspected actinobacterium was appeared, then isolated and coded as SM1P. SM1P was characterized based on morphological and biochemical traits and identified as a genus of Streptroporangium then called Streptroporangium sp. SM1P. Streptroporangium sp. SM1P was carried out anti-bacterial assay on both

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S, Sarah, and Shanmugharaju , V. "Bacterial Protease Inhibitors as Antibacterial agents to prevent Bacterial Infections Associated with Biofilms." Journal of University of Shanghai for Science and Technology 23, no. 10 (2021): 398–412. http://dx.doi.org/10.51201/jusst/21/10730.

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Isolation of protease inhibitor producing bacteria from microbial mat and investigating its anti-biofilm potential against biofilm producing organism was selected as the main objective of the present study. Protease inhibitor (PI) was produced from bacterial isolates and purified using ammonium sulphate precipitation methods. Primary and secondary protease inhibitor assay was carried out separately to confirm the inhibition of protease enzyme activity both qualitatively and quantitatively. Antibacterial activity and anti-biofilm assay was performed to determine the biofilm prevention capabilit

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O. Elansary, Hosam, Agnieszka Szopa, Marta Klimek-Szczykutowicz, et al. "Mammillaria Species—Polyphenols Studies and Anti-Cancer, Anti-Oxidant, and Anti-Bacterial Activities." Molecules 25, no. 1 (2019): 131. http://dx.doi.org/10.3390/molecules25010131.

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Discovering new natural resources of polyphenols is the aim of many recent studies in the field of natural product research. This study tentatively investigated the polyphenols profile of the stems of seven Mammillaria species (M. rhodantha, M. spinosissima, M. hahniana, M. crucigera, M. candida, M. albilanata, and M. muehlenpfordtii) using high performance liquid chromatography with DAD detector (HPLC-DAD) method. Furthermore, the anti-cancer, anti-oxidant, and anti-bacterial potentials of these extracts as well as major identified phenols were explored. The HPLC-DAD study confirmed the avail

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Al-Jubouri, A. K., N. H. Al-Saadi, and M. A. Kadhim. "ANTI- INFLAMMATORY AND ANTI- BACTERIAL ACTIVITY OF COPPER NANOPARTICLES SYNTHESIZED FROM MYRTUS COMMUNIS LEAVES EXTRACT." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 53, no. 3 (2022): 698–711. http://dx.doi.org/10.36103/ijas.v53i3.1580.

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A variety of organisms, including plants and bacteria, fungi, seaweeds, and microalgae, are involved in the biological synthesis of nanoparticles. Copper nanoparticles (CuNPs) can be synthesized using plant extracts, which is considered to be one of the safest methods in green chemistry. Copper-NPs were synthesized from the leaves of M. communis, which were extracted with water. The first indication that CuNPs have been synthesized is the change in color of the solution from light yellow to dark brown. Several different techniques were used to characterize CuNPs. The Surface Plasmon Resonance

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8

Kavitha, .S, B. Baijika., Mathew Cyril, K. P. Jithamol, and Purandharan Aswani. "A Review of Coumarin as Antibacterial Agent." Journal of Advances in Pharmaceutical Sciences 1, no. 2 (2023): 32–44. https://doi.org/10.5281/zenodo.10089048.

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<i>Coumarin is the heterocyclic compound formed from benzene and pyrone ring containing oxygen atom. Coumarin derivatives have different biological activities such as anticancer, antimicrobial, antimalarial, antioxidant, antiviral, anti-inflammatory analgesic, antianxiety etc. The synthesised coumarin derivatives were screened for their anti-bacterial activity by means of Disc-well diffusion assay against Gram-positive bacteria and Gram-negative strains. The compounds proved that it is moderate to significant anti-bacterial activity. This review is based on the synthesis of various coumar

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Said, Bibie, Loveness Charlie, Emnet Getachew, Catherine Lydiah Wanjiru, Mekdelawit Abebe, and Tsegahun Manyazewal. "Molecular bacterial load assay versus culture for monitoring treatment response in adults with tuberculosis." SAGE Open Medicine 9 (January 2021): 205031212110334. http://dx.doi.org/10.1177/20503121211033470.

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The lack of rapid, sensitive, and deployable tuberculosis diagnostic tools is hampering the early diagnosis of tuberculosis and early detection of treatment failures. The conventional sputum smear microscopy or Xpert MTB/RIF assay cannot distinguish between alive and dead bacilli and the culture method delays providing results. Tuberculosis molecular bacterial load assay is a reverse transcriptase real-time quantitative polymerase chain reaction that quantifies viable tuberculosis bacillary load as a marker of treatment response for patients on anti-tuberculosis therapy. However, results are n

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10

Emrich, Stefanie, Anja Schuster, Thomas Schnabel, and Gertie Janneke Oostingh. "Antimicrobial Activity and Wound-Healing Capacity of Birch, Beech and Larch Bark Extracts." Molecules 27, no. 9 (2022): 2817. http://dx.doi.org/10.3390/molecules27092817.

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Bark is a major by-product of woodworking industries. The contents of several wood species are known to harbor antimicrobial, antiviral, anti-inflammatory and wound-healing capacities. The aim of this work was to identify beneficial properties of Austrian larch, birch and beech bark extracts for their potential usage as additives or active ingredients in dermatological applications. Bacterial agar diffusion assay and resazurin-based broth microdilution assay were used to evaluate anti-bacterial activity. To gain more insight into the cellular response to bark extracts, viability-, scratch-assa

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Sheu, Shew-Meei, Bor-Shyang Sheu, Hsiao-Bai Yang, Huan-Yao Lei, and Jiunn-Jong Wu. "Anti-Lewis X Antibody Promotes Helicobacter pylori Adhesion to Gastric Epithelial Cells." Infection and Immunity 75, no. 6 (2007): 2661–67. http://dx.doi.org/10.1128/iai.01689-06.

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ABSTRACT Lewis X (Lex) antigen is expressed on the human gastric mucosa and the O-specific chain of lipopolysaccharides of Helicobacter pylori. This antigen can induce autoantibodies, which may be involved in bacterial colonization and thus deserve further investigation. Flow cytometry was used to examine the effects of anti-Le monoclonal antibodies (MAbs) on H. pylori adhesion. A babA2 mutant was also constructed to evaluate the effect of an anti-Lex MAb on adhesion. The bacterial agglutination and in situ adhesion assays were used to confirm the anti-Lex MAb effect on H. pylori adhesion. Thi

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12

Yu, Moxi, Yachen Hou, Meiling Cheng, et al. "Antibacterial Activity of Squaric Amide Derivative SA2 against Methicillin-Resistant Staphylococcus aureus." Antibiotics 11, no. 11 (2022): 1497. http://dx.doi.org/10.3390/antibiotics11111497.

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Methicillin-resistant Staphylococcus aureus (MRSA)-caused infection is difficult to treat because of its resistance to commonly used antibiotic, and poses a significant threat to public health. To develop new anti-bacterial agents to combat MRSA-induced infections, we synthesized novel squaric amide derivatives and evaluated their anti-bacterial activity by determining the minimum inhibitory concentration (MIC). Additionally, inhibitory activity of squaric amide 2 (SA2) was measured using the growth curve assay, time-kill assay, and an MRSA-induced skin infection animal model. A scanning elect

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13

Pattni, Ramesh, Hitesh Kumarkhaniya, Bharat Maitreya, and Nainesh Modi. "PHYTOCHEMICAL ANALYSIS, ANTI-OXIDANT ACTIVITY AND ANTI-BACTERIAL ACTIVITY OF SALVADORA OLEOIDES L." International Association of Biologicals and Computational Digest 2, no. 1 (2023): 183–90. http://dx.doi.org/10.56588/iabcd.v2i1.127.

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Salvadora oleoides L. is a facultative and mesomorphic xerophyte adapted to arid and semi-arid regions. It is commonly known as meetha jal in Gujarat and Rajasthan. These plant species populations were grown in different ecological regions such as Punjab, Rajasthan, and Kutch. The present study analyzed the presence of secondary metabolites and also their amount. The anti-oxidant assay such as DPPH and ABTS determine the reducing agent which is present in plant. Anti-bacterial activity checked against the pseudomonas bacteria is 5 mm (Zone of inhibition) at concentration of 1 mg/ml.

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14

Rambaran, Neervana, Yougasphree Naidoo, Farzana Mohamed, Hafizah Y. Chenia, and Himansu Baijnath. "Antibacterial and Anti-Quorum Sensing Properties of Silver Nanoparticles Phytosynthesized Using Embelia ruminata." Plants 13, no. 2 (2024): 168. http://dx.doi.org/10.3390/plants13020168.

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The rise in antibiotic resistance (AR) poses an imminent threat to human health. Nanotechnology, together with mechanisms such as quorum sensing (QS), which relies on communication between bacterial cells, may decrease the selective pressure for AR. Thus, this study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) synthesized at room temperature (Rt) and 80 °C using Embelia ruminata leaf, stem-bark, and fruit extracts as antibacterial and anti-QS agents. The phytosynthesized AgNPs solutions were subjected to various characterization assays and assessed for their antibacte

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15

Amiin, Muhammad Kholiqul, and Almira Fardani Lahay. "Anti-Bacterial Effectiveness Of Cymodocea Rotundata Extract And Assay For Primary Bioactive Composition." Journal of Aquatropica Asia 8, no. 1 (2023): 6–12. http://dx.doi.org/10.33019/joaa.v8i1.3920.

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Anti-bacterial is a compound that can be used to inhibit bacterial growth. The compounds that play a role in damaging the cell membrane are phenols, flavonoids, and alkaloids. The phytochemical compounds above have the potential as natural Anti-bacterial on pathogenic bacteria, for example against Escherichia coli. Escherichia coli is a pathogenic microbe in humans that can cause digestive disorders and disrupt the work system of the stomach organs. Seagrass C. rotundata has compounds that are antibacterial, such as alkaloids, flavonoids, phenols, steroids, and tannins. C. rotundata can be see

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Baszczynski, Chris L. "A rapid immunoprecipitation assay for neomycin phosphotransferase II expression in transformed bacteria and plant tissues." Biochemistry and Cell Biology 68, no. 6 (1990): 983–87. http://dx.doi.org/10.1139/o90-145.

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Anti-kanamycin antibodies produced in rabbits, following coupling of the antibiotic to bovine serum albumin, were used to immunoprecipitate radioactively labelled phosphorylated kanamycin from transformed bacterial or plant extracts in a novel assay system, for the detection of neomycin phosphotransferase II (NPTII) activity. Radioactive counts in the immunoprecipitated pellet give a semiquantitative measure of the kanamycin phosphorylation and hence the amount of NPTII activity. This assay is sensitive, uses very small amounts of radioactivity, and is very rapid, allowing many samples to be p

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Saranya, J., S. Preethi, Mohammed Rafi Shaik, Merajuddin Khan, Mujeeb Khan, and Baji Shaik. "Development of Cerium Oxide/Chitosan/Graphene Oxide Nanocomposite: An Investigation toward Its Biological Applications under In Vitro Conditions." Crystals 13, no. 9 (2023): 1393. http://dx.doi.org/10.3390/cryst13091393.

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A ternary nanocomposite was prepared using cerium oxide, chitosan, and graphene oxide (CeO2/CS/GO) using a simple and cost-effective wet chemical method. The physicochemical properties of the developed ternary nanocomposite were examined using X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy with energy-dispersive X-ray spectroscopy, and ultraviolet-visible spectroscopy. Furthermore, the therapeutic behavior of the developed CeO2/CS/GO composite was assessed using anti-bacterial, anti-fungal, and anti-cancer assays. For Escherichia coli, Staphylococcus a

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Wu, Meng, Bingjian Zhang, Guoping Sun, and Leping Jiang. "Determination of lacquer contained in samples of cultural relics by enzyme-linked immunosorbent assay." New Journal of Chemistry 41, no. 14 (2017): 6226–31. http://dx.doi.org/10.1039/c7nj00831g.

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19

Schaller, Alain, Zhonghe Sun, Yongping Yang, Akos Somoskovi, and Ying Zhang. "Salicylate Reduces Susceptibility of Mycobacterium tuberculosis to Multiple Antituberculosis Drugs." Antimicrobial Agents and Chemotherapy 46, no. 8 (2002): 2636–39. http://dx.doi.org/10.1128/aac.46.8.2636-2639.2002.

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ABSTRACT Salicylate induces multiple antibiotic resistance in various bacterial species. Here we investigated the effect of salicylate on the susceptibility of Mycobacterium tuberculosis to a range of antituberculosis (anti-TB) drugs. In the presence of salicylate, the killing effects of isoniazid (INH), rifampin (RMP), ethambutol (EMB), streptomycin (STR), and p-aminosalicylate (PAS) were reduced, as shown with a tetrazolium redox dye viability assay and a bacterial survival assay. Salicylate-induced resistance was more pronounced for PAS, STR, and EMB but was not apparent for INH and RMP whe

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Nalina Kumari, Chellathurai Burnice, Namasivayam Ambalavanan, Shanmugam Rajesh Kumar, Jaideep Mahendra, and Uma Sudhakar. "Anti-oxidant, anti-inflammatory and antimicrobial activity of aqueous extract from acerola and amla." Bioinformation 20, no. 7 (2024): 765–70. http://dx.doi.org/10.6026/973206300200765.

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Amla, scientifically known as emblica officinalis and Acerola (malphigian emarginata) both are Vitamin C fruits possess varied medicinal properties being used for preventive disease health management strategies. Therefore, it is of interest to explore the antioxidant, anti-inflammatory, antibacterial, and cytotoxic properties of aqueous extracts from Acerola and Amla. Hence, the anti-inflammatory activity of Acerola and amla was assessed using the bovine serum albumin denaturation assay (BSA Assay), antioxidant properties were compared using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Both ext

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Ökmen, Gülten, Zafer Cantekin, Mohammmad Intakhab Alam, Onur Türkcan, and Yaşar Ergün. "Antibacterial and Antioxidant Activities of Liquidambar Orientalis Mill. Various Extracts Against Bacterial Pathogens Causing Mastitis." Turkish Journal of Agriculture - Food Science and Technology 5, no. 8 (2017): 883. http://dx.doi.org/10.24925/turjaf.v5i8.883-887.1163.

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Antibiotic resistance is being constantly developed worldwide. Coagulase Negative Staphylococci (CNS) and Staphylococcus aureus are common causes of bovine subclinical mastitis. Bioactive compound of medicinal plants shows anti-microbial, anti-mutagenic and anti-oxidant effects. The anti-bacterial and anti-oxidant activities of Liquidambar orientalis (L. orientalis) extracts on subclinical mastitis causing bacteria in cows have not been reported to date. The aim of the present study was to examine anti-bacterial and anti-oxidant effects of L. orientalis leaf extracts on S. aureus and CNS isola

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Maheswari, P., K. Arjun Kumar, S. Ramya, M. Venkatesh, and B. Vigneshwaran. "The Characterization and Analysis of Medicinal Plant Extracts Against Nosocomial Infection." Journal of Drug Delivery and Therapeutics 9, no. 4 (2019): 126–34. http://dx.doi.org/10.22270/jddt.v9i4.3086.

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The plant extracts are effective for the various aspects in the field of biology. There has been an increasing interest world-wide on therapeutic values of natural products. The extracts of Trigonella and Phyllanthus are observed to have anti-bacterial and anti-fungal activity. The clinical pathogens showed growth inhibition to the both extracts. The extracts showed inhibition of zone in Staphylococci and E coli. The combined effect was observed to have anti-fungal effect. The extracts were analysed for the anti-oxidant assays which showed fine amount of scavenging of the free radicals in tota

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Akhtar, Sultan, Suriya Rehman, Munirah A. Almessiere, Firdos Alam Khan, Yassine Slimani, and Abdulhadi Baykal. "Synthesis of Mn0.5Zn0.5SmxEuxFe1.8−2xO4 Nanoparticles via the Hydrothermal Approach Induced Anti-Cancer and Anti-Bacterial Activities." Nanomaterials 9, no. 11 (2019): 1635. http://dx.doi.org/10.3390/nano9111635.

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Manganese metallic nanoparticles are attractive materials for various biological and medical applications. In the present study, we synthesized unique Mn0.5Zn0.5SmxEuxFe1.8−2xO4 (0.01 ≤ x ≤ 0.05) nanoparticles (NPs) by using the hydrothermal approach. The structure and surface morphology of the products were determined by X-ray powder diffraction (XRD), transmission electron and scanning electron microcopies (TEM and SEM), along with energy dispersive X-ray spectroscopy (EDX). We evaluated the impact of Mn0.5Zn0.5SmxEuxFe1.8−2xO4 NPs on both human embryonic stem cells (HEK-293) (normal cells)

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Sachin, B. Ghar, and K. Das Yamilee. "The green & eco-friendly biosynthesized biocompatible metallic silver nanoparticles for anti-bacterial and anti-biofilm efficacy." European Journal of Biotechnology and Bioscience 10, no. 01 (2022): 43–49. https://doi.org/10.5281/zenodo.6860383.

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The existing work was based on a prudent & environmentally affordable outlook for a green biological mode of synthesis of nanoparticles (NPs) in an aqueous solvent system. In order to biologically synthesized NPs, the plant leaves were used. Subsequently, the anti-bacterial assessment of NPs evaluated the bacterial activities of multidrug-resistant clinical isolates. The bacterial growth rate, minimum inhibitory concentration (MIC), & biofilm formation were explored in S. aureus clinical isolates. The results exposed that NPs were more effective with varying anti-bacterial potential ag

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Abuei, Haniyeh, Abbas Behzad-Behbahani, Fatemeh Faghihi, et al. "The Effect of Bacterial Peptide p28 on Viability and Apoptosis Statusof P53-null HeLa Cells." Advanced Pharmaceutical Bulletin 9, no. 4 (2019): 668–73. http://dx.doi.org/10.15171/apb.2019.078.

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Purpose: Despite all the efforts for discovery of efficient anti-cancer therapeutics, cancer is stilla major health concern worldwide. p28 is a bacterial small peptide which has been widelyinvestigated due to its preferential cell internalization and anti-cancer activities. Intracellularly,p28 offers its anti-cancer traits by impeding the degradation of tumor-suppressor protein "p53".In this study, we investigated the potency of p28 in inducing apoptosis or decreasing cellviability in p53-null "HeLa" cell line.Methods: The coding sequence for p28 peptide was obtained from Pseudomonas aeruginos

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Yust-Katz, Shlomit, Elinor Gigi, Deborah Rosenberg, et al. "TAMI-40. TUMOR MICROBIOME AND GLIOBLASTOMA (GBM)." Neuro-Oncology 22, Supplement_2 (2020): ii221—ii222. http://dx.doi.org/10.1093/neuonc/noaa215.928.

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Abstract Bacteria have been found to play major roles in many physiologic/disease processes including cancer. The presence of bacteria within brain tumors has never been explored. The aim of this study was to examine the microbiome of Glioblastoma. A cohort of 40 glioblastoma samples (FFPE), from two medical centers served for DNA extraction using a specialized extraction protocol that includes a bead beating step to ensure complete bacterial DNA recovery. A set of negative controls was introduced at different steps of the assay to identify and monitor contaminating bacterial DNA. We measured

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Saravanan, Renuka, Ravichandran Natesan, Sumathi C Samiappan, and Sivakumar Ramalingam. "Anti-Oxidant, Anti-Bacterial and Anti-Cancer Activity of Mentha Piperita Against Mcf-7 Cells." Biomedical and Pharmacology Journal 14, no. 3 (2021): 1685–93. http://dx.doi.org/10.13005/bpj/2270.

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The study evaluates the antioxidant, antibacterial and anticancer activities of different extracts of Menthapiperitaleaves against the MCF-7 cell line. The leaves of Menthapiperitawere extracted with aqueous, ethyl acetate, and chloroform. These extracts were subjected to qualitative phytochemical screening, antibacterial activity, cytotoxic activity, and AO/ErBr assay for cells' apoptotic effect against the MCF-7 cell line. Qualitative analysis of the leaves' different extracts revealed glycosides, alkaloids, flavonoids, terpenoids, tannin, and saponin. The antibacterial activity of the leaf

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Haj-Yahya, Farah, Doron Steinberg, and Ronit Vogt Sionov. "Trans, Trans-Farnesol Enhances the Anti-Bacterial and Anti-Biofilm Effect of Arachidonic Acid on the Cariogenic Bacteria Streptococcus mutans and Streptococcus sobrinus." International Journal of Molecular Sciences 25, no. 21 (2024): 11770. http://dx.doi.org/10.3390/ijms252111770.

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Background: Streptococcus mutans and Streptococcus sobrinus are Gram-positive bacteria involved in the development of dental caries, as they are able to form biofilms on tooth enamel, ferment sugars into acids, and survive under acidic conditions. This ultimately leads to a local lowering of the pH value on the tooth surface, which causes enamel cavities. Hypothesis: One measure to reduce caries is to limit the growth of cariogenic bacteria by using two anti-bacterial agents with different mechanisms of action. The hypothesis of this study was that the anti-bacterial activity of ω-6 polyunsatu

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Mihalache, Madalina, Alina Banciu, Lucian Ionescu, and Mihai Nita-Lazar. "Elisa preliminary studies of immobilization and specific detection of bacterial strains." Romanian Journal of Ecology & Environmental Chemistry 2, no. 2 (2020): 64–69. http://dx.doi.org/10.21698/rjeec.2020.209.

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The paper aims to emphasize the specific detection of bacterial strains using enzyme-linked immunosorbent assay. The assay is based on the specific binding of polyclonal antibody anti-E. coli tagged with FITC to E.coli and monoclonal antibody anti-Ps. aeruginosa tagged with Alexa Fluor 647 tagged to Ps. aeruginosa and on subsequent enzymatic immunological demonstration of the conjugated enzyme. In this experiment, the negative control was the Salmonella enterica strain. The two antibodies had no interaction with the negative control, instead, they were specific for E. coli and Ps. aeruginosa s

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Xie, Huijun, Chao Niu, Zeyang Chao, Nuramina Mamat, and Haji Akber Aisa. "Synthesis and Activity of New Schiff Bases of Furocoumarin." Heterocyclic Communications 26, no. 1 (2020): 176–84. http://dx.doi.org/10.1515/hc-2020-0115.

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AbstractFurocoumarins, such as 8-MOP, are the most common medications used to relieve the symptoms of vitiligo clinically. Some furocoumarins also showed excellent performance in an anti-bacterial assay. This paper describes the synthesis of a series of novel Schiff bases (6a-6k), and their promotion in melanogenesis and anti-bacterial properties were studied in vitro.The pigment production of B16 cells and bacterial inhibition ring assay were applied for the bioactivity of 6a-6k. According to the results, a stronger promotion on pigment content was observed, when six compounds co-cultured wit

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Janosch, D., S. Dubbert, K. Eiteljörge, et al. "Anti-genotoxic and anti-mutagenic activity of Escherichia coli Nissle 1917 as assessed by in vitro tests." Beneficial Microbes 10, no. 4 (2019): 449–61. http://dx.doi.org/10.3920/bm2018.0113.

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Anti-genotoxic or anti-mutagenic activity has been described for a number of Gram-positive probiotic bacterial species. Here we present evidence that Gram-negative Escherichia coli Nissle 1917 (EcN) also displays anti-genotoxic/anti-mutagenic activity, as assessed in vitro by the Comet Assay and the Ames Test, respectively. This activity was demonstrated by use of the mutagens 4-nitroquinoline-1-oxide (NQO), hydrogen peroxide (H2O2) and benzo(a) pyrene (B[a]P). For both assays and all three test agents the anti-genotoxic/anti-mutagenic activity of EcN was shown to be concentration dependent. B

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Kaur, Atinderpal, Reema Gabrani, and Shweta Dang. "Nanoemulsions of Green Tea Catechins and Other Natural Compounds for the Treatment of Urinary Tract Infection: Antibacterial Analysis." Advanced Pharmaceutical Bulletin 9, no. 3 (2019): 401–8. http://dx.doi.org/10.15171/apb.2019.047.

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Purpose: Nanoemulsions (NEs) of polyphenon 60 (P60) and cranberry (NE I) and P60 and curcumin (NE II) were prepared with the aim to enhance anti-bacterial potential and to understand the mechanism of anti-bacterial action of the encapsulated compounds. Methods: To evaluate the antibacterial potential of the developed NE, microtiter biofilm formation assay was performed. The cytotoxicity analysis was done to assess the toxicity profile of the NEs. Further antibacterial analysis against uropathogenic strains was performed to check the developed NEs were effective against these strains. Results:

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Clare, Rachel H., Darren A. N. Cook, Kelly L. Johnston, Louise Ford, Stephen A. Ward, and Mark J. Taylor. "Development and Validation of a High-Throughput Anti-Wolbachia Whole-Cell Screen." Journal of Biomolecular Screening 20, no. 1 (2014): 64–69. http://dx.doi.org/10.1177/1087057114551518.

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There is an urgent need to develop new, safe, and affordable macrofilaricidal drugs for onchocerciasis and lymphatic filariasis treatment and control. The Anti- Wolbachia Consortium (A·WOL) aims to provide a novel treatment with macrofilaricidal activity by targeting the essential bacterial symbiont Wolbachia. The consortium is currently screening a diverse range of compounds to find new chemical space to drive this drug discovery initiative and address this unmet demand. To increase the throughput and capacity of the A·WOL cell-based screen, we have developed a 384-well format assay using a h

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Maidment, Clem, Zosia Dembny, and Paul King. "Investigations into the anti-bacterial properties of garlic using the disc assay method." Journal of Biological Education 32, no. 3 (1998): 162–65. http://dx.doi.org/10.1080/00219266.1998.9655616.

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Parveen, Romana, Tooba Naz Shamsi, Himanshu Kumar, and Sadaf Fatima. "PHYTOCHEMICAL ANALYSIS AND IN VITRO BIOLOGICAL CHARACTERIZATION OF AQUEOUS AND METHANOLIC EXTRACT OF BACOPA MONNIERI." International Journal of Pharmacy and Pharmaceutical Sciences 8, no. 12 (2016): 90. http://dx.doi.org/10.22159/ijpps.2016v8i12.14739.

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<p><strong>Objective</strong>:<strong> </strong>The present study was designed to identify the phytocompounds, to compare the antibacterial, antioxidant, and anti-inflammatory effects of aqueous and methanolic extract of <em>Bacopa monnieri</em>.</p><p><strong>Methods</strong>:<strong> </strong>Antioxidant activity was determined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), Super oxide dismutase (SOD), Reduced glutathione (GSH), Catalase assays. Anti-inflammatory activity was me

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Matotoka, Mashilo Mash, and Peter Masoko. "Evaluation of the Antioxidant, Cytotoxicity, Antibacterial, Anti-Motility, and Anti-Biofilm Effects of Myrothamnus flabellifolius Welw. Leaves and Stem Defatted Subfractions." Plants 13, no. 6 (2024): 847. http://dx.doi.org/10.3390/plants13060847.

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The formation of biofilms underscores the challenge of treating bacterial infections. The study aimed to assess the antioxidant, cytotoxicity, antibacterial, anti-motility, and anti-biofilm effects of defatted fractions from Myrothamnus flabellifolius (resurrection plant). Antioxidant activity was assessed using DPPH radical scavenging and hydrogen peroxide assays. Cytotoxicity was screened using a brine shrimp lethality assay. Antibacterial activity was determined using the micro-dilution and growth curve assays. Antibiofilm potential was screened using the crystal violet and tetrazolium redu

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Rajapaksha, RMHKK, EMN Fernando, AWMKK Bandara, NRM Nelumdeniya, and ARN Silva. "In-vitro Anti-bacterial Activity of Methanol and Aqueous Crude Extracts of Horsfieldia iryaghedhi." Asian Plant Research Journal 12, no. 4 (2024): 27–34. http://dx.doi.org/10.9734/aprj/2024/v12i4259.

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Aims: Over the past two decades, the rise of multidrug resistance (MDR) in bacteria has posed a significant threat to global health. The urgent need for new treatment alternatives has brought attention to the potential of plants, which harbor a wealth of unexplored phytochemicals with therapeutic properties. This study aims to evaluate the anti-bacterial efficacy of methanol and aqueous extracts from the leaves and bark of Horsfieldia iryaghedhi In vitro. Methodology: Aqueous and methanol extracts were obtained from the cold maceration method. In vitro anti-bacterial activity of methanol and a

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Shalini, Kumar Gautam Surendra, Srivastava Shobhit, and Kumar Gupta Sujeet. "Indole: A Versatile Heterocyclic." Chemistry Research Journal 6, no. 4 (2021): 17–30. https://doi.org/10.5281/zenodo.11665549.

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<strong>Abstract </strong>Indole is a heterocyclic compound comprise of benzene and pyrrole ring. The Indole nucleus plays an important role in medicinal chemistry to synthesized a series of multiple biological active derivatives such as Anti-inflammatory, Anti-proliferative, Anti-bacterial, Antidiabetic, Antioxidant, Antifungal, Anticancer etc. Various indole derivatives and substituents are mention in article. These derivative of carbazole are tested for varying pharmacological activities. The main motive of the review article to describe their pharmacological action and activities against d

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Zhu, Yifan, Hiroki Kawai, Satoshi Hashiba, et al. "The Effect of GD1a Ganglioside-Expressing Bacterial Strains on Murine Norovirus Infectivity." Molecules 25, no. 18 (2020): 4084. http://dx.doi.org/10.3390/molecules25184084.

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In this study, we investigated the impact of GD1a-expressing bacterial strains on the infectivity of murine norovirus (MNV). Eligible bacterial strains were screened from a sewage sample using flow cytometry, and their genetic sequences of 16S rRNA were determined. The enzyme-linked immunosorbent assay (ELISA) was employed to analyze the binding between bacteria and MNV particles, and the plaque assay was used to assess the effects of GD1a-positive and negative strains on MNV infectivity. The result from ELISA shows that MNV particles are able to bind to both GD1a-positive and negative bacteri

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Heneghan, Michael A., Ciaran F. McCarthy, Daiva Janulaityte, and Anthony P. Moran. "Relationship of Anti-Lewis x and Anti-Lewis y Antibodies in Serum Samples from Gastric Cancer and Chronic Gastritis Patients toHelicobacter pylori-Mediated Autoimmunity." Infection and Immunity 69, no. 8 (2001): 4774–81. http://dx.doi.org/10.1128/iai.69.8.4774-4781.2001.

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ABSTRACT Lewis (Le) antigens have been implicated in the pathogenesis of atrophic gastritis and gastric cancer in the setting ofHelicobacter pylori infection, and H. pylori-induced anti-Le antibodies have been described that cross-react with the gastric mucosa of both mice and humans. The aim of this study was to examine the presence of anti-Le antibodies in patients with H. pylori infection and gastric cancer and to examine the relationships between anti-Le antibody production, bacterial Le expression, gastric histopathology, and host Le erythrocyte phenotype. Anti-Le antibody production and

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Kicheeva, Arina G., Ekaterina S. Sushko, Lyubov S. Bondarenko, et al. "Functionalized Magnetite Nanoparticles: Characterization, Bioeffects, and Role of Reactive Oxygen Species in Unicellular and Enzymatic Systems." International Journal of Molecular Sciences 24, no. 2 (2023): 1133. http://dx.doi.org/10.3390/ijms24021133.

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The current study evaluates the role of reactive oxygen species (ROS) in bioeffects of magnetite nanoparticles (MNPs), such as bare (Fe3O4), humic acids (Fe3O4-HA), and 3-aminopropyltriethoxysilane (Fe3O4-APTES) modified MNPs. Mössbauer spectroscopy was used to identify the local surrounding for Fe atom/ions and the depth of modification for MNPs. It was found that the Fe3O4-HA MNPs contain the smallest, whereas the Fe3O4-APTES MNPs contain the largest amount of Fe2+ ions. Bioluminescent cellular and enzymatic assays were applied to monitor the toxicity and anti-(pro-)oxidant activity of MNPs.

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Martı́nez-Govea, A., J. Ambrosio, L. Gutiérrez-Cogco, and A. Flisser. "Identification and Strain Differentiation of Vibrio cholerae by Using Polyclonal Antibodies against Outer Membrane Proteins." Clinical Diagnostic Laboratory Immunology 8, no. 4 (2001): 768–71. http://dx.doi.org/10.1128/cdli.8.4.768-771.2001.

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ABSTRACT Cholera is caused only by O1 and O139 Vibrio cholerae strains. For diagnosis, 3 working days are needed for bacterial isolation from human feces and for biochemical characterization. Here we describe the purification of bacterial outer membrane proteins (OMP) from V. cholerae O1 Ogawa, O1 Inaba, and O139 strains, as well as the production of specific antisera and their use for fecal Vibrio antigen detection. Anti-OMP antisera showed very high reactivity and specificity by enzyme-linked immunosorbent assay (ELISA) and dot-ELISA. An inmunodiagnostic assay for V. cholerae detection was d

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Halder, Surabhi, and Rajesh Kumar S. "ANTI-INFLAMMATORY AND ANTI-OXIDANT ACTIVITY OF HAFNIUM OXIDE NANOPARTICLES SYNTHESISED USING MOMORDICA CHARANTIA." International Journal of Advanced Research 13, no. 02 (2025): 590–99. https://doi.org/10.21474/ijar01/20409.

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Background:Green synthesised Hafnium oxide nanoparticles can be prepared for various other applications to address oral health concerns like bacterial infections and inflammation.The current study explored the anti-inflammatory and antioxidant activity of the synthesized Hafnium Oxide Nanoparticles. Materials and Methods: Hafnium Oxide nanoparticles were prepared using Momordica Charantia plant extract and tested for potential applications.The anti-inflammatory activity of the produced nanoformulation was examined using a bovine serum albumin denaturation assay, egg albumin denaturation assay

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Baker, Brett. "1289. Pravibismane is a Potent, Broad Spectrum Anti-Infective Small Molecule that Rapidly Disrupts Bacterial Bioenergetics and Halts Bacterial Growth." Open Forum Infectious Diseases 7, Supplement_1 (2020): S659—S660. http://dx.doi.org/10.1093/ofid/ofaa439.1472.

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Abstract Background The rise in resistance to existing antimicrobials has prompted a need for the development of novel antibiotics. Microbion has identified a novel compound, pravibismane, with potent broad spectrum anti-infective and anti-biofilm activity. Methods Here we used a variety of assays, including Bacterial Cytological Profiling (BCP), to analyze pravibismane in E.coli to gain insight into its likely mechanism of action (MOA). The BCP profile of pravibismane suggested it rapidly shut down cell growth, potentially by turning off cellular gene or protein expression. This was confirmed

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Xie, Yanxuan, Jingxin Chen, Bo Wang, Ai-Yun Peng, Zong-Wan Mao, and Wei Xia. "Inhibition of Quorum-Sensing Regulator from Pseudomonas aeruginosa Using a Flavone Derivative." Molecules 27, no. 8 (2022): 2439. http://dx.doi.org/10.3390/molecules27082439.

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Quorum sensing (QS) is a cell-to-cell communication process that controls bacterial collective behaviors. The QS network regulates and coordinates bacterial virulence factor expression, antibiotic resistance and biofilm formation. Therefore, inhibition of the QS system is an effective strategy to suppress the bacterial virulence. Herein, we identify a phosphate ester derivative of chrysin as a potent QS inhibitor of the human pathogen Pseudomonas aeruginosa (P. aeruginosa) using a designed luciferase reporter assay. In vitro biochemical analysis shows that the chrysin derivative binds to the b

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Tirgar, Pravin, Jinal Adhiya, Sougata Ghosh, and Swati Goswami. "In-vitro and In-vivo Wound Healing Acceleration by Biogenic Silver Nanoparticles." E3S Web of Conferences 619 (2025): 04003. https://doi.org/10.1051/e3sconf/202561904003.

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Aim: In-vivo and In-vitro evaluation of potential biogenically (from plant Dioscorea bulbifera, Dioscorea oppositifolia, Plumbago zeylanica, Gloriosa superba) prepared silver nanoparticles (AgNPs) in wound healing process. Materials and methods: In-vitro anti-bacterial activity of D. bulbifera, D. oppositifolia, P. zeylanica and G. superba prepared AgNPs in Gram-positive and Gram-negative bacterial pathogens performed using cup and plate assay. In-vitro phagocytic stimulatory assay used to find out potential antibacterial activity of AgNPs. In-vivo pharmacological evaluation of inflammatory po

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Di, Da, Mythili Dileepan, Shamim Ahmed, Yuying Liang, and Hinh Ly. "Recombinant SARS-CoV-2 Nucleocapsid Protein: Expression, Purification, and Its Biochemical Characterization and Utility in Serological Assay Development to Assess Immunological Responses to SARS-CoV-2 Infection." Pathogens 10, no. 8 (2021): 1039. http://dx.doi.org/10.3390/pathogens10081039.

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The SARS-CoV-2 nucleocapsid protein (N) binds a single-stranded viral RNA genome to form a helical ribonucleoprotein complex that is packaged into virion particles. N is relatively conserved among coronaviruses and consists of the N-terminal domain (NTD) and C-terminal domain (CTD), which are flanked by three disorganized regions. N is highly immunogenic and has been widely used to develop a serological assay as a diagnostic tool for COVID-19 infection, although there is a concern that the natural propensity of N to associate with RNA might compromise the assay’s specificity. We expressed and

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Mensah, John Kenneth, and Philip Teye Thompson. "Anti-oxidant, anti-microbial and anti-inflammatory activities of <i>Saba thompsonii</i> fruit extracts." International Journal of Biological and Chemical Sciences 17, no. 4 (2023): 1323–40. http://dx.doi.org/10.4314/ijbcs.v17i4.4.

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Aqueous extracts of the fruits of Saba thompsonii are used topically in ethnomedicine in Ghana for healthy wound healing and wound repair. The objective of this study was to comparatively assess the methanolic and the diethylether extracts of the plant phytochemicals for three bioactivities indicative of wound repairs. Using standard laboratory assays involving broth dilution, carrageenan-induced foot swelling of 7-day old chicks and DPPH radical scavenging, this study assessed the anti-microbial, anti-inflammatory and anti-oxidant activities of the methanolic and the diethylether extracts of

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Wibawa, I. P. A. H., M. Hanafi, A. S. Li’aini та ін. "Total phenolic and flavonoid contents of Aphanamixis polystachya (Wall.) R.Parker leaf extract and its potential as antioxidant and inhibitor of α-glucosidase". IOP Conference Series: Earth and Environmental Science 1255, № 1 (2023): 012016. http://dx.doi.org/10.1088/1755-1315/1255/1/012016.

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Abstract Indonesia is one of the countries with the highest plant diversity in the world, including the Aphanamixis genus, and they use the plants in this genus as traditional medicine. The genus Aphanamixis has a wide variety of compounds with different activities, such as insecticidal, leishmanicidal, antimicrobial, anticancer, cytotoxic against some cancer cells, and antioxidant The focus of this research is to find out about the anti-oxidant, anti-diabetic, and anti-bacterial activities of Aphanamixis polystachya leaf extract from the Bali Botanical Garden plant collection. The antioxidant

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Rashtchian, A., J. Eldredge, M. Ottaviani, et al. "Immunological capture of nucleic acid hybrids and application to nonradioactive DNA probe assay." Clinical Chemistry 33, no. 9 (1987): 1526–30. http://dx.doi.org/10.1093/clinchem/33.9.1526.

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Abstract Antibodies specific for DNA:RNA hybrids were coated onto polystyrene test tubes and applied to hybridization assays involving DNA and RNA. Synthetic DNA probes complementary to 16S rRNA of Campylobacter were labeled with biotin and hybridized to ribosomal RNA directly in lysates of bacterial cells. After hybridization, DNA:RNA hybrids were captured with immobilized anti-DNA:RNA antibody, and the biotinylated probe was detected with streptavidin-horseradish peroxidase (EC 1.11.1.7) conjugate. The assay was optimized to detect as few as 70,000 Campylobacter cells in a sample. We compare

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