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Journal articles on the topic 'Anti-globulin gel test'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Cate, John C., and Nancy Reilly. "Evaluation and Implementation of the Gel Test for Indirect Antiglobulin Testing in a Community Hospital Laboratory." Archives of Pathology & Laboratory Medicine 123, no. 8 (1999): 693–97. http://dx.doi.org/10.5858/1999-123-0693-eaiotg.

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Abstract Background.—The gel test, developed by Lapierre in 1984, was designed to standardize antiglobulin testing while improving sensitivity and specificity of the method. Principle.—Anti–human serum immunoglobulin G (IgG) mixed with Sephadex G100 (gel phase) in a microtube traps red cell–IgG agglutination complexes during migration through the gel in a centrifugation step. Agglutination complexes are visibly detectable at various levels in the microtube as an inverse function of antibody coated on red cells. Unsensitized red cells form a cell pellet at the base of the microtube. Objective.—

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2

Samarah, Fekri, Mahmoud A. Srour, Dirgham Yaseen, and Kamal Dumaidi. "Frequency of Red Blood Cell Alloimmunization in Patients with Sickle Cell Disease in Palestine." Advances in Hematology 2018 (June 6, 2018): 1–7. http://dx.doi.org/10.1155/2018/5356245.

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Background. Transfusion of red blood cells (RBC) is an essential therapeutic tool in sickle cell disease (SCD). Repeated RBC transfusions can cause alloimmunization which causes difficulty in cross-matching and finding compatible blood for transfusions. This study aimed to investigate the frequency of RBC alloimmunization and related risk factors among Palestinian SCD patients.Materials and Methods. A multicenter cross-sectional study on 116 previously transfused SCD patients from three centers in West Bank, Palestine. Demographic, medical data and history of transfusion were recorded. Blood s

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3

Bujandric, Nevenka, Jasmina Grujic, and Zorana Budakov-Obradovic. "Red blood cell alloimmunization in pregnancy: A 10-year single-centre study." Vojnosanitetski pregled, no. 00 (2021): 16. http://dx.doi.org/10.2298/vsp201124016b.

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Background/Aim. Pregnancy-induced red blood cell (RBC) alloimmunization is important not only because of the possible negative effects on subsequent pregnancy outcomes, in case the fetus carries the antigen, but also because of the optimal transfusion management in cases of obstetric haemorrhage. Timely detection of RBC antibodies is part of a testing, prevention and treatment strategy aimed at achieving better outcomes for alloimmunized mothers with an affected fetus. The aim was to determine the frequency and specificity of alloantibodies among pregnant women from the South Backa District, w

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4

Yurtsever, Nalan, Chris Tormey, Laurie Bizzario, and Edward Lee. "Evaluating RhD Assessment By Automated Methodology: A Potential ‘Blind Spot’ For RhD Variant Identification." American Journal of Clinical Pathology 162, Supplement_1 (2024): S163. http://dx.doi.org/10.1093/ajcp/aqae129.360.

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Abstract Background Automated blood typing platforms are ideal for blood donor centers, given their broad recognition of RhD antigen variants, resulting in products from such donors to be labeled as RhD positive. However, this ‘blind spot’ for variants may be problematic for hospital-based transfusion services, since RhD variants may require provision of RhD- RBC units and Rh immune globulin. The extent to which automated methods accurately characterize variant D antigen expression is not known. As such, the objective of our study was to assess D typing results, as well as RHD genotyping, amon

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5

Lee, Oukseub, Xinlei Mi, Yanfei Xu, et al. "Abstract PD15-12: PD15-12 A pre-surgical window trial of oral tamoxifen versus transdermal 4-hydroxytamoxifen gel in women with estrogen receptor positive duct carcinoma in situ (DCIS)." Cancer Research 83, no. 5_Supplement (2023): PD15–12—PD15–12. http://dx.doi.org/10.1158/1538-7445.sabcs22-pd15-12.

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Abstract Background: Adjuvant oral tamoxifen (TAM) benefits women with DCIS, but toxicity concerns have limited its acceptance. Transdermal therapy with 4-hydroxy tamoxifen (4-OHT) gel applied to the breast skin is a possible solution. Previous pilot data suggest equivalent anti-proliferative efficacy of TAM and 4-OHT gel, but minimal systemic exposure with transdermal therapy. We report a prospective double blinded randomized phase 2 trial comparing TAM to 4-OHT gel in women with DCIS. Methods: 107 women with estrogen receptor positive (≥10%) DCIS were randomized to TAM (20 mg/day + placebo g

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6

Jain, Ashish, Sharanya Ramakrishnan, Parmatma Prasad Tripathi, Rekha Hans, Deepak Bansal, and Ratti Ram Sharma. "Transfusion management in a pediatric patient with febrile neutropenia with red blood cell autoantibodies: a case report." International Journal of Research in Medical Sciences 9, no. 12 (2021): 3696. http://dx.doi.org/10.18203/2320-6012.ijrms20214722.

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Febrile neutropenia is a common complication of chemotherapy especially in hematological malignancies associated with sepsis or severe infection. We report a case where a seven-year-old girl with T – cell acute lymphoblastic leukemia (ALL) developed febrile neutropenia (absolute neutrophil count - ANC <500/µL). Patient developed transient red blood cell (RBC) autoantibodies which interfered with compatibility testing and posed a challenge in donor selection for granulocyte transfusion. Direct antiglobulin test (DAT) and compatibility testing were done by column agglutination technique (CAT)

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7

Lee, Oukseub, Latifa Bazzi, Yanfei Xu, et al. "Abstract RF01-02: A randomized Phase I pre-operative window trial of transdermal endoxifen in women planning mastectomy: evaluation of dermal safety, intra-mammary drug distribution, and biologic effects." Cancer Research 84, no. 9_Supplement (2024): RF01–02—RF01–02. http://dx.doi.org/10.1158/1538-7445.sabcs23-rf01-02.

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Abstract Background: Breast cancer prevention requires only local exposure of the breast to active drug but oral preventive agents entail systemic exposure, with resultant adverse effects, limiting acceptance by high-risk women. Drug-delivery through the breast skin is an attractive option, but requires demonstration of dermal safety and drug distribution throughout the breast. We formulated the tamoxifen metabolite (E/Z)-endoxifen in a hydroalcoholic/oleic acid gel for transdermal delivery. We tested this in a placebo-controlled, double-blinded Phase I trial with dose escalation from 10 to 20

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8

Thant, Mamie Myo, and Alesia Kaplan. "The Impact of the "Super Coombs" Test on Patient Management and Outcomes at a Large Tertiary Care Center: A Retrospective Review." Blood 138, Supplement 1 (2021): 1073. http://dx.doi.org/10.1182/blood-2021-149287.

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Abstract Background: The direct antiglobulin test (DAT) identifies the presence of immunoglobulins and complement on the surface of red blood cells and is used to differentiate immune from non-immune hemolysis. Standard DATs done by conventional methods, using anti-IgG or anti-C3 antihuman globulin (AHG) in tube or gel, are performed routinely by laboratories associated with hospital transfusion services. However, if the standard DAT is negative but a strong clinical suspicion for immune-mediated hemolysis remains, serologic testing at an immunohematology reference lab (IRL) can be performed.

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9

Chari, Ajai, Toshihisa Satta, Amit Tayal, et al. "Outcomes and Management of Red Blood Cell Transfusions in Multiple Myeloma Patients Treated with Daratumumab." Blood 126, no. 23 (2015): 3571. http://dx.doi.org/10.1182/blood.v126.23.3571.3571.

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Abstract Background Daratumumab (DARA) is a human IgG1 kappa monoclonal antibody that targets cells expressing CD38, which is highly expressed in multiple myeloma (MM) cells. In the phase 2 MMY2002 (Sirius) trial, treatment with single agent DARA resulted in a 29% overall response rate, with a median PFS of 3.7 months in heavily pretreated MM patients (Lonial S. J Clin Oncol. 2015; 33(suppl): abstrLBA8512). However, CD38 is also expressed on red blood cells (RBCs) and DARA binding to RBCs results in pan-reactivity on RBC panel testing using an indirect antiglobulin test (IAT). Recently, it was

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10

Vaillant, Angel Alberto Justiz, Patrick Eberechi Akpaka, Norma Mc Farlane-Anderson, and Monica P. Smikle. "Use of Staphylococcal Protein-A and Streptococcal Protein-G for Detection of Red Blood Cells (RBC) Antibodies and Comparison with Other Techniques." May 24, 2013. https://doi.org/10.5281/zenodo.8003.

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Background: Detection of red blood cells antibodies is important for the diagnosis of autoimmune hemolytic anemia, hemolytic disease of newborn, pre-transfusion testing and other problems. The aim of this study was to use Staphylococcal protein A (SpA) and Streptococcal protein G (SpG) as reagents in immunological tests for detecting red blood cells (RBC) antibodies and to compare the method with other techniques. Study Design & Methods: Sera from 60 patients, comprising forty-four anti-D positive sera from pregnant women and 16 from healthy controls were, used for the study. The anti-glob

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11

V, Ranjitha, Vijay C, and Shashidhara T S. "Gel Card and Saline Tube Techniques for Blood Cross-Matching: A Comparative Assessment Study." RGUHS Journal of Allied Health Sciences 2, no. 2 (2022). http://dx.doi.org/10.26463/rjahs.2_2_3.

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Background Gel card technique of cross matching is one of the latest techniques in blood bank. It has high sensitivity and gives accurate results. The aim of this study is to compare the sensitivity of gel card method with the conventional saline tube method for cross matching in blood bank.Methodology In this study we used Matrix gel card method based on indirect coombs test ICT for cross match and tube method including spin saline tube method with anti-human globulin AHG and without AHG at Mallasandra urban primary health care center for pre-transfusion cross matching on 100 blood samples.Re

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12

Pandey, Aditi, Sonal Chandrakar, Roshan Lal, and Kajal Chandrakar. "Blood cross-matching using gel card technique compared to conventional tube technique: A diagnostic evaluation study." MRIMS Journal of Health Sciences, July 26, 2024. http://dx.doi.org/10.4103/mjhs.mjhs_30_24.

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Abstract Background: Conventional tube technique (CTT) is the most commonly used method for blood cross-matching in India. The gel card technique is the latest technique emerged and due to its accurate results, it is considered a method of choice for cross-matching. Objective: The objective of the study was to compare blood cross-matching using the gel card technique compared to CTT with and without anti-human globulin (AHG). Materials and Methods: A diagnostic evaluation study was conducted on 1500 blood samples. Patients’ blood samples were sent to the blood bank for routine cross-matching p

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13

Decaro, Jorge, Angel Pedrozo, Martín Gularte, Gustavo Tironi, and Estela Citrín. "Absence of hemolytic disease of fetus and newborn (HDFN) in a pregnancy with anti-Yka (York) red cell antibody." Case Reports in Perinatal Medicine 1, no. 1-2 (2012). http://dx.doi.org/10.1515/crpm-2012-0003.

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AbstractThe York antigen, assigned to the Knops system (KN5-ISBT 022005), is a high frequency antigen present in 90% of the Caucasian and 98% of the African-American population. No cases of anti-Yka in pregnancies have been published. No hemolytic diseases of the fetus and newborn have been observed previously. We report the first case of anti-Yka antibody found in a pregnant woman without fetal anemia, which was monitored by Doppler assessment of peak systolic velocity at the middle cerebral artery. A 36-year-old white woman, gravida 2, para 2 (1994 and 1996) was transfused with two units of

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14

Keerthi, Chinthapeta, Rajendran Arun, Bandi Suresh Babu, et al. "Serological Characterisation of Auto-antibodies in Patients with Direct Antiglobulin Test Positive Autoimmune Haemolytic Anaemia at a Tertiary Care Teaching Hospital in Tirupati, India." JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2021. http://dx.doi.org/10.7860/jcdr/2021/50542.15247.

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Introduction: Haemolysis in Autoimmune Haemolytic Anaemia (AIHA) is a result of Immunoglobulin G (IgG) or Immunoglobulin M (IgM) auto-antibodies with or without complement components binding to the Red Blood Cell (RBC) surface and initiating its destruction. Serologic evidence is provided by autocontrol or Direct Antiglobulin Test (DAT). Diagnostic work-up is essential as the management depends on the antibody type. Characteristics of the bound antibody and the target antigen determine the degree of haemolysis. Serological characterisation in AIHA helps to differentiate into its various types

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15

Schönbacher, Marlies, Chiara Banfi, Andrea Berghold, et al. "Immunoglobulin Class Profiles of ABO Antibodies in Saliva and Serum of Healthy Individuals." Transfusion Medicine and Hemotherapy, November 29, 2022, 1–9. http://dx.doi.org/10.1159/000527233.

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<b><i>Introduction:</i></b> The coronavirus disease (COVID-19) pandemic gave rise to studies investigating the association of ABO blood group with COVID-19 susceptibility. It is hypothesized that ABO antibodies might play a role in neutralizing SARS-CoV-2. However, ABO antibodies were exclusively analyzed in blood samples. Investigation of ABO antibodies in saliva, an easy-to-obtain surrogate for respiratory secretions, may provide novel insights into mucosal immunity crucial in early defense against respiratory pathogens. <b><i>Methods:</i></b>

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16

Singh, Akriti, Satish Belagatti, Brilsee Simeon, and Rashmi Budha. "Prevalence of Weak D Antigen in Rh Negative Blood Group: An Experience at a Tertiary Blood Centre in Bengaluru, Southern India." NATIONAL JOURNAL OF LABORATORY MEDICINE, 2023. http://dx.doi.org/10.7860/njlm/2023/64521.2807.

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Introduction: The Rh blood group system, consisting of more than 50 different antigens, is the most polymorphic among human blood types. Among them, D is the most important antigen. The incidence of weak (Du) antigens ranges from 0.2 to 1%. Routine screening for RhD does not cause agglutination with anti-RhD serum; therefore, it must be detected using anti- human globulin serum. Due to the potential immunological reaction of Du-positive cells in RhD-negative individuals, it is crucial to emphasise the therapeutic implications of this finding and its relation to the risk of alloimmunisation. Ai

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17

Hattori, Naoki, Takeshi Matsuda, Kazuhisa Chihara, Junko Nishioka, Selin Elmaoğulları, and Akira Shimatsu. "SAT-426 Macro-Thyroid Stimulating Hormone (TSH) in Children." Journal of the Endocrine Society 4, Supplement_1 (2020). http://dx.doi.org/10.1210/jendso/bvaa046.725.

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Abstract Macro-TSH is mainly a complex of TSH with anti-TSH autoantibodies. Due to its large molecular size (&gt;150 kDa), it accumulates in the circulation resulting in elevated serum TSH concentrations. Because the bioactivity of macro-TSH is low, treatment with thyroxine is not necessary. The prevalence of macro-TSH is no more than 1% in adult patients with subclinical hypothyroidism. However, the prevalence of macro-TSH in children is not known. We report here two cases of macro-TSH in pediatric setting. [Case reports] Case 1. Six-year and eight-month-Japanese boy visited a pediatric h

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