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Dissertations / Theses on the topic 'Anti-idiotype'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Lefèvre, Gilbert. "Anticorps anti-idiotype et hormone anti-mullerienne." Paris 7, 1989. http://www.theses.fr/1989PA077083.

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Des anticorps anti-idiotype polyclonaux ont ete prepares contre trois anticorps monoclonaux diriges contre l'hormone anti-mullerienne bovine. Tous les anticorps anti-idiotypes inhibent la fixation d'hormone anti-mullerienne marquee sur l'anticorps monoclonal contre lequel ils ont ete prepares

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2

Elliott, T. J. "Interaction of anti-idiotype antibodies with the surface of neoplastic lymphocytes." Thesis, University of Southampton, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373970.

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3

Macbeth, F. R. "Diagnostic and therapeutic uses of anti-idiotype antibody in the management of human B-cell lymphoma." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375173.

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4

ZAGHOUANI, HABIB. "Induction chez la souris d'une reponse contre le dextrane b1355s par sensibilisation avec des anticorps anti-idiotypiques." Paris 7, 1987. http://www.theses.fr/1987PA077019.

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5

Chang, Shang-Hung. "Generation and testing of an anti-idiotype that acts as a surrogate antigen for oxidized LDL (OxLDL) in serological studies measuring anti-OxLDL antibodies." Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/11743.

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Atherosclerosis is thought to be mediated by immune response in which oxidized low density lipoprotein (OxLDL) is one of the major antigens. The exact linkage between anti-OxLDL antibodies and atherosclerosis, however, is controversial. One of the sources of this controversy is the inherently heterogeneous and complex nature of OxLDL as an antigen. This study aims to identify and characterise a new type of structurally definable surrogate antigen for OxLDL for use in serologic assays. The study applied two techniques for cloning antibodies. The first step was the generation of a monoclonal anti-OxLDL antibody, designated LO-1, by fusing mouse B cells and myeloma cells. The mouse used in this fusion was not immunized but fed with high fat diet to reflect the physiologic but hyperlipidemic immune environment. The second step was to use a phage display system (Tomlinson library of single chain variable antibody fragments) to screen anti-idiotypic antibodies (anti-id) against an anti-OxLDL monoclonal antibody generated from that mouse. The anti-id selected for further characterisation, was designated H3. This anti-id, which probably mimics both peptide and lipid components of OxLDL, was used as an antigen in a serologic assay (both in mice and humans) and compared with ordinary OxLDL antigens conventionally used by earlier researchers. Titres of antibodies against H3 correlated well with antibodies against malondialdehyde-conjugated LDL. Although the overall performance of anti-H3 antibodies was no better than conventional anti-OxLDL assays in predicting atherosclerosis, they showed an inverse relationship with the extent of left anterior descending coronary artery stenosis in patients with established coronary artery disease. This project has developed a novel approach to measuring OxLDL antibodies. The proposed unique mimicry of both the lipoprotein and the phospholipid components of OxLDL may reveal a functionally significant epitope of OxLDL that has not been successfully mapped by previously reported methodologies. The novel anti-id H3 and the corresponding mimetic region of OxLDL therefore provide new insight and new tools for future research on the humoral response related to atherosclerosis.

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6

Solassol, Isabelle. "Analyse de deux antigènes associés aux tumeurs solides, l'antigène carcinoembryonnaire (ACE) et l'antigène erbB-2 : étude des épitopes continus de l'ACE : production de fragments scFv humains anti-idiotypes anti-erbB-2 en vue de la mise au point d'un vaccin anti-tumoral." Montpellier 1, 2000. http://www.theses.fr/2000MON1T023.

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7

JOLIMOY, CYRILLE. "Sensibilisation de la recherche des anticorps anti-idiotypes par absorption des anticorps anti-hla de classe i sur plaquettes : mise au point des cross-matches par cytometrie en flux." Dijon, 1994. http://www.theses.fr/1994DIJOM088.

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8

Holm, Patrik. "Single-chain antibody construction and functional mapping of the monoclonal antibody TS1 : Its interaction with the antigen and the anti-idiotype." Licentiate thesis, Karlstad University, Division for Chemistry, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-2323.

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<p>The aims of this study are to synthesize and produce a single-chain antibody (scFv) of the anti-cytokeratin 8 monoclonal IgG antibody TS1 and to functionally map amino acid residues important for the interaction with its antigen and the anti-idiotypic antibody TS1. The TS1 antibody has been shown to be effective in binding cytokeratin 8 (CK8) expressed in tumors in vivo and is proposed to be useful in immunotargeting and/or immunotherapy. The anti-idiotypic antibody TS1 can be used to regulate the tumor:non-tumor ratio. Mutagenesis of certain amino acid residues can be used to alter the affinity to improve the tumor:non-tumor ratio further.</p><p>In the present study, the TS1 IgG was chemically modified to specify groups of residues important for interaction with both CK8 and TS1. If important residues were found in the CDRs, they were mutated in the TS1 scFv construct and the effect was studied using ELISA.</p><p>The main conclusions drawn from this study are that the important amino acid residues in TS1 for the interaction with both CK8 and TS1 are mainly tyrosines, charged residues and a tryptophan. A central interacting interface was identified with the somewhat unusual participation of residues in the CDR 2 of the light chain. Mutations which resulted in increased affinity to both CK8 and TS1 were also identified.</p>

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9

Holm, Patrik. "Functional mapping and in vivo metabolism of the monoclonal antibody TS1 and its single-chain fragment : Its interaction with the antigen and the anti-idiotype." Doctoral thesis, Karlstad University, Faculty of Technology and Science, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-727.

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<p>Antibodies are proteins capable of specific interactions to a wide range of molecules. These interactions are facilitated by the complementary determining regions (CDR).</p><p>Carcinomas are the most common of human cancers and they release significant amount of cytokeratins (CK) in the necrotic areas of the tumors. The CKs stay in the tumor, since they have low solubility. The antibody studied in this thesis, the anti-CK 8 antibody TS1, has shown to be effective in tumor targeting and is proposed to be useful in therapy.</p><p>Single-chain antibodies (scFv) are recombinant antibodies which are much smaller than the intact IgG. This is an advantage when used in tumor therapy, since they can penetrate the tumors more easily than the larger IgG. Moreover, they are expressed by one single gene which make them easy to modify, for example by site-directed mutagenesis.</p><p>The anti-idiotypic antibody αTS1 can be used to clear the TS1 form the circulation and thereby clear the body from non-tumor bound TS1 in therapy. To be able to modify the binding of an antibody to its antigen and or anti-idiotype, these interactions must be studied. In this study this is accomplished by chemical modifications of the IgGs TS1 and αTS1 and the antigen CK 8. Guided by these results, amino acid residues were mutated by using site-directed mutagenesis in the TS1-218 scFv and the effects were studied. From mutational study results, the functional epitope could be mapped and it was found that there are mainly tyrosines, but also charged residues, serine and a tryptophan that are important for both interactions. The binding of TS1-218 to both αTS1 and CK 8 could be improved by changing the negatively charged side-chains by mutations to their corresponding amide or alanine.</p><p>Both the IgG and scFv versions of TS1 were administered in vivo. The IgG αTS1 was used to clear the TS1 from the circulation by forming immune complexes. The immune complexes, consisting of four or more antibodies, were mainly metabolized by the liver. The scFv TS1-218 could localize to the tumor in a tumor xenograft mouse model, although a higher uptake would be desired in a therapeutic strategy. The scFv was cleared rapidly by the kidneys, but the clearance could be slowed by pre-formed immune complexes with anti-TS1 scFv in vitro, prior to administration in vivo.</p>

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10

Furtado, Patricia Brotto. "Production and Characterisation of a chimaeric human IgE antibody, recognising der P 1, and its chimaeric human IgG1 anti-idiotype : the prospect of idiotypic-anti-idiotypic interactions as an intervention strategy for allergy." Thesis, University of Nottingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368255.

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11

Couraud, Pierre-Olivier. "Anticorps antiidiotypiques diriges contre le recepteur beta-adrenergique et le recepteur de l'angiotensine ii." Paris 7, 1987. http://www.theses.fr/1987PA077027.

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12

Chamat, Soulaima. "Etude structurale, génétique et idiotypique de la réponse anti-ligands beta-adrénergiques chez la souris." Paris 7, 1985. http://www.theses.fr/1985PA077017.

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13

Fadda, Valeria. "Structural studies on a hepatitis C virus-related immunological complex and on Ebola virus polymerase cofactor VP35." Thesis, University of St Andrews, 2015. http://hdl.handle.net/10023/7703.

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Hepatitis C virus (HCV) is one of the leading causes of hepatocellular carcinoma worldwide. HCV-neutralizing antibody AP33 recognizes a linear, highly conserved epitope on the viral entry protein E2, disrupting the interaction with the cellular receptor CD81 that leads to viral entry. AP33-related anti-idiotypes (Ab₂s) have the potential to carry the internal image of the antigen E2, eliciting the production of AP33-like antibodies in humans. This study reports the mid-resolution structure of the Fab fragment of anti-idiotype A164.3 and the high-resolution structure of the Fab fragment of AP33 in complex with the Fv fragment of anti-idiotype B2.1A. Analysis of the structures and comparison with the previously published structure of AP33 in complex with a peptide corresponding to the E2 epitope, suggests that while A164.3 does not mimic the antigen E2, B2.1A is characterized by high surface complementarity with AP33 and functional antigen mimicry. Thus, B2.1A can be classified as an Ab₂-β, a subgroup of anti-idiotypes carrying the internal image of the antigen. Preliminary binding studies show that AP33 binds B2.1A with nanomolar affinity, supporting the role of B2.1A as an idiotypic vaccine candidate. Zaire ebola virus causes severe, often lethal hemorrhagic fever in humans. Ebola virus polymerase cofactor VP35 is a multifunctional protein involved in, among other functions, dsRNA binding and inhibition of the host's interferon pathways. VP35 contains an N-terminal oligomerization domain and a C-terminal dsRNA-binding domain (RBD). Preliminary results on the oligomerization domain of VP35 suggest that this region contains a coiled-coil motif, as previously reported. In order to validate a recently-discovered dsRNA end-capping pocket as a drug target, the structure of VP35 RBD I278A mutant was solved at high resolution, showing that even a small perturbation in the binding pocket can cause dramatic binding impairment due to loss of contacts with dsRNA.

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14

Bennay, Lai͏̈la. "Les anticorps anti-idiotypiques." Bordeaux 2, 1993. http://www.theses.fr/1993BOR2PE50.

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15

Hsu, Kuo-Hui. "Idiotype, anti-idiotype and anti-anti-idiotype antibodies for aflatoxin production, characterization and application /." 1995. http://catalog.hathitrust.org/api/volumes/oclc/32632632.html.

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16

D'Auvergne, Oswald. "Idiotype anti-iodiotype an experimental schistosomiasis vaccine." 1993. http://books.google.com/books?id=qy5YAAAAMAAJ.

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17

Benguric, Dubravka Roka. "Anti-idiotype antibodies and phage displayed peptides as antigenic mimics of a Mycoplasma capricolum subsp. capripneumoniae epitope." Diss., 2001. http://hdl.handle.net/2263/23600.

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Contagious caprine pleuropneumonia (CCPP) is a highly contagious disease that affects goats in Africa and Asia resulting in great economic losses. The aetiological agent is Mycoplasma capricolum subsp. capripneumoniae (Mccp). It belongs to a group of organisms that are all associated with economically important diseases of ruminants and all exhibit similar genetic and antigenic characteristics. The diagnosis of CCPP has often been considered difficult due to confusion with other mycoplasmas of ruminants and the limited specificity of most diagnostic assays. It is therefore important to improve diagnosis and thereby the control of CCPP. Two approaches, namely phage display and anti-idiotype antibodies can be used to identify antigenic mimics with the potential to be used in developing new vaccines, diagnostic methods or therapeutics. Both were used in an attempt to generate surrogate antigens for Mccp. A monoclonal antibody (Mab) or its F( ab ')2 fragments directed against Mccp membrane protein epitopes was injected into hens to induce the production of anti-idiotype antibodies. The antibodies produced were functionally able to mimic the epitope recognised by the Mab since they inhibited binding of the Mab to mycoplasmal lysate. Mice were also immunised with the Mab and or F(ab') 2 fragments of the antibody. The resulting antisera were tested in ELISA, but no significant response was detected. The selection of peptides from a random epitope library displayed on the surface of filamentous phages was used to characterise the epitope recognised by the Mab. Two different, but related peptides were identified that reacted with the antibody in enzyme-linked immunosorbent assays (ELISA). Binding to the Mab was further characterised by surface plasmon resonance. Sequence analysis revealed that the two peptides each had a cysteine residue in addition to the one fixed in amino acid position 2 as well as identical or similar amino acid residues in positions 5 (P), 8 (I/L) and 13 (L). One of the peptides had 74% similarity with an amino acid sequence of the PG 1 strain of Mycoplasma mycoides subsp. mycoides SC. The peptides as well as the anti-idiotype antibodies were not detectable using Mccp-specific goat antiserum suggesting that the serum did not contain paratopes that could accommodate the surrogate epitopes. In spite of this, the antiserum efficiently inhibited the binding of the Mab to immobilised mycoplasmal lysate in a standardised test for Mccp antibodies. This assay therefore appears to depend on a structural, rather than a functional blocking of the epitope which the Mab recognises. The findings in this study have elucidated some of the characteristics of the Mab and raised the possibility that the epitope recognised by the Mab is not immunodominant. Peptides identified by phage display and chicken/murine anti-idiotype antibodies, nevertheless, have the potential of being used as antigens in immunoassays aimed at CCPP diagnosis. In light of the results generated it would therefore be necessary to investigate other Mabs or polyclonal antiserum in order to yield antibodies or peptides of the desired specificity.<br>Dissertation (MSc (Microbiology))--University of Pretoria, 2007.<br>Microbiology and Plant Pathology<br>unrestricted

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