Academic literature on the topic 'Antigen-antibody reactivity'

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Journal articles on the topic "Antigen-antibody reactivity"

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Bentley, G. A., G. Boulot, and V. Chitarra. "Cross-reactivity in antibody-antigen interactions." Research in Immunology 145, no. 1 (1994): 45–48. http://dx.doi.org/10.1016/s0923-2494(94)80042-1.

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Bobrovnik, S. A., M. O. Demchenko, and S. V. Komisarenko. "Effect of trifluoroethanol on antibodies binding properties." Ukrainian Biochemical Journal 95, no. 1 (2023): 20–30. http://dx.doi.org/10.15407/ubj95.01.020.

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The studies on the influence of organic co-solvents on the structure and function of antibodies are of key interest, especially in view of antibodies broad use as recognizing elements in different analytical systems. Here we studied the effect of co-solvent 2,2,2-trifluoroethanol (TFE) on the ability of anti-ovalbumin monoclonal antibodies to interact with its specific antigen. Antibody affinity to antigen and the rate constants of antibody binding to immobilized antigen were analyzed. Changes in antibody reactivity with incubation time which depended on TFE concentration and temperature were
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Brüssow, H., and J. Sidoti. "Reactivity of human serum antibody with lipopolysaccharide O 78 antigen from enterotoxigenicEscherichia coli." Epidemiology and Infection 108, no. 2 (1992): 315–22. http://dx.doi.org/10.1017/s0950268800049785.

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SUMMARYFifteen and five of 20 volunteers challenged with the enterotoxigenicEscherichia colistrain O 78·H11 showed a fourfold titre increase of serum ELISA antibody to the homologous O 78 and the heterologous O 8 lipopolysaccharide antigen, respectively. Sixty-three of 191 sera from 1- to 48-month-old German children showed serum antibody reactive with O 78 antigen, all but two of these O 78-positive sera also showed reactivity with at least one further O antigen. Only 14 of the O 78 reactive sera also showed antibody to heat-labile enterotoxin. In addition, soluble O 8 antigen could inhibit t
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Thomas, C. B., D. E. Jasper, J. T. Boothby, and J. D. Dellinger. "Enzyme-linked immunosorbent assay for detection of Mycoplasma californicum-specific antibody in bovine serum: Optimization of assay determinants and control of serologic cross-reactions." American Journal of Veterinary Research 48, no. 4 (1987): 590–95. https://doi.org/10.2460/ajvr.1987.48.04.590.

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SUMMARY An enzyme-linked immunosorbent assay (elisa) was adapted to detect Mycoplasma californicum-specific antibodies in bovine serum. Cross-reactive antibody was found in the M californicum-positive reference serum when assayed against each of 7 solid-phase antigens of heterologous mycoplasma species. Cross-reactivity was further demonstrated by inhibition of elisa reactivity to M californicum solid-phase antigen by incubation of sera with antigen suspensions of each heterologous species. Incubation of test sera with a cross-reacting antigen mixture containing equal proportions of the 7 cros
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Valdarnini, Niccolò, Bettina Holm, Paul Hansen, Paolo Rovero, Gunnar Houen, and Nicole Trier. "Fine Mapping of Glutamate Decarboxylase 65 Epitopes Reveals Dependency on Hydrophobic Amino Acids for Specific Interactions." International Journal of Molecular Sciences 20, no. 12 (2019): 2909. http://dx.doi.org/10.3390/ijms20122909.

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Characterization of multiple antibody epitopes has revealed the necessity of specific groups of amino acid residues for reactivity. This applies to the majority of antibody–antigen interactions, where especially charged and hydrophilic amino acids have been reported to be essential for antibody reactivity. This study describes thorough characterization of glutamic acid decarboxylase (GAD) 65 antigenic epitopes, an immunodominant autoantigen in type 1 diabetes (T1D). As linear epitopes are sparsely described for GAD65 in T1D, we aimed to identify and thoroughly characterize two GAD65 antibodies
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Inman, R. D., B. Chiu, and N. C. Hamilton. "Analysis of immune complexes in rheumatoid arthritis for Epstein-Barr virus antigens reveals cross-reactivity of viral capsid antigen and human IgG." Journal of Immunology 138, no. 2 (1987): 407–12. http://dx.doi.org/10.4049/jimmunol.138.2.407.

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Abstract We recently defined the immunochemical characteristics of immune complexes (IC) isolated from synovial fluid (SF) of patients with rheumatoid arthritis with the use of Western blot analysis. In the present study, we probe for exogenous antigens in the IC by examining the specificity of antisera raised against the IC. Anti-IC antisera demonstrated strong reactivity against the viral capsid antigen (VCA) of Epstein Barr virus (EBV), which was not explained by preimmune reactivity, polyclonal B cell activation, or Fc-mediated binding in the immunofluorescence or ELISA systems used to mea
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Papp, Krisztián, Ágnes Kovács, Anita Orosz, et al. "Absolute Quantitation of Serum Antibody Reactivity Using the Richards Growth Model for Antigen Microspot Titration." Sensors 22, no. 10 (2022): 3962. http://dx.doi.org/10.3390/s22103962.

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In spite of its pivotal role in the characterization of humoral immunity, there is no accepted method for the absolute quantitation of antigen-specific serum antibodies. We devised a novel method to quantify polyclonal antibody reactivity, which exploits protein microspot assays and employs a novel analytical approach. Microarrays with a density series of disease-specific antigens were treated with different serum dilutions and developed for IgG and IgA binding. By fitting the binding data of both dilution series to a product of two generalized logistic functions, we obtained estimates of anti
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Larrea, Carlos Fernández de, Jacobus Henry de Waard, Francesca Giampietro, and Zaida Araujo. "The secretory immunoglobulin A response to Mycobacterium tuberculosis in a childhood population." Revista da Sociedade Brasileira de Medicina Tropical 39, no. 5 (2006): 456–61. http://dx.doi.org/10.1590/s0037-86822006000500007.

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We report on the measurement of saliva anti-Purified Protein Derivative sIgA and 38kDa antibodies from 127 children, of whom 31 were strong tuberculosis suspects and 96 were healthy contact children. The results concerning the percentage of children with antibody reactivity to PPD and 38kDa antigens showed that, of these 2 antigens, 38kDa induced higher reactivity in patients positive and negative for the Tuberculin Skin Test (28% and 16.6%, respectively) in comparison to controls positive and negative for the TST (11.7% and 7.1%, respectively). There was a statistically significant difference
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Burnett, Deborah L., Peter Schofield, David B. Langley, et al. "Conformational diversity facilitates antibody mutation trajectories and discrimination between foreign and self-antigens." Proceedings of the National Academy of Sciences 117, no. 36 (2020): 22341–50. http://dx.doi.org/10.1073/pnas.2005102117.

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Conformational diversity and self-cross-reactivity of antigens have been correlated with evasion from neutralizing antibody responses. We utilized single cell B cell sequencing, biolayer interferometry and X-ray crystallography to trace mutation selection pathways where the antibody response must resolve cross-reactivity between foreign and self-proteins bearing near-identical contact surfaces, but differing in conformational flexibility. Recurring antibody mutation trajectories mediate long-range rearrangements of framework (FW) and complementarity determining regions (CDRs) that increase bin
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Devine, Peter L., Rachel J. Quin, Paul W. Shield, Yew Wah Liew, John K. Sheehan, and David J. Thornton. "Monoclonal Antibody Recognizing a Core Epitope on Mucin." Disease Markers 14, no. 2 (1998): 99–112. http://dx.doi.org/10.1155/1998/678434.

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Monoclonal antibody TH1 (IgM) was prepared by immunizing mice with deglycosylated (TFMSA-treated) cystic fibrosis mucin. TH1 reacted strongly with TFMSA treated cystic fibrosis mucin but not with the fully glycosylated mucin, indicating reactivity with a core mucin epitope. TH1 showed no reactivity with ovine mucin (98% of glycans as sialyl-Tn) but reacted strongly with desialylated ovine mucin, indicating the epitope for this mab was the Tn-antigen (O-linked GalNAc). However, TH1 showed no reactivity with Tn-positive red blood cells, and the binding of TH1 was not inhibited by GalNAc at 2.5 m
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Dissertations / Theses on the topic "Antigen-antibody reactivity"

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Mummert, Mark E. "Stability in antigenic reactivity of the major outer surface protein, OspA, in borrelia burgdorferi, during persistent infection in Syrian hamsters." Virtual Press, 1992. http://liblink.bsu.edu/uhtbin/catkey/845968.

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The spirochete Borrelia burgdorferi is the causative agent of Lyme disease, a multisystem disorder that can cause a variety of disorders in susceptible mammalian hosts. The immune response of infected mammals, including humans, is ineffective in clearing B. burgdorferi as demonstrated by the ability to reisolate the spirochete from naturally and experimentally infected hosts after extended periods of time. Recent evidence suggests that this pathogen evades the immune response in part through changes in antigenic reactivity.The purpose of this study was to determine if outer surface protein A (
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Forsström, Björn. "Characterization of antibody specificity using peptide array technologies." Doctoral thesis, KTH, Proteomik och nanobioteknologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-155723.

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Antibodies play an important role in the natural immune response to invading pathogens. The strong and specific binding to their antigens also make them indispensable tools for research, diagnostics and therapy. This thesis describes the development of methods for characterization of an- tibody specificity and the use of these methods to investigate the polyclonal antibody response after immunization. Paper I describes the development of an epitope-specific serum fractionation technique based on epitope map- ping using overlapping peptides followed by chromatographic separation of polyclonal s
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Hardy, Gregory. "Model Membranes Study the Lipid-Reactivity of HIV-1 Antibodies and Vaccine Antigen." Diss., 2014. http://hdl.handle.net/10161/8711.

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<p>One promising HIV-1 vaccine target is the membrane-proximal external region (MPER) of viral gp41. MPER is poorly immunogenic, however, the two rare neutralizing antibodies (NAbs), 2F5 and 4E10, bind to MPER with great neutralizing ability. Although their neutralizing mechanism represents a promising framework for the design of new HIV-1 liposomal vaccine candidates, this mechanism remains poorly understood. It is known that 2F5 and 4E10 are required to first associate with HIV-1 lipids before binding to the target MPER antigen, however, little is known about how lipid membranes contribute t
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Books on the topic "Antigen-antibody reactivity"

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Tatalick, Lauren Marie. Protective immunity to Cryptosporidium parvum: Efficacy of antibody and spleen cell subsets enriched for reactivity to surface antigen-1. 1994.

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Book chapters on the topic "Antigen-antibody reactivity"

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Osbourn, Jane K. "Selection of antibodies from phage libraries of immunoglobulin genes." In Monoclonal Antibodies. Oxford University PressOxford, 2000. http://dx.doi.org/10.1093/oso/9780199637232.003.0003.

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Abstract This chapter explains the various approaches it is possible to take in the selection of phage antibodies with specific binding characteristics from large phage display libraries. The particular selection approach taken will be determined by the form and availability of the starting antigen and the desired properties of the selected antibody. One of the main benefits of the phage display approach to antibody generation is the ability to tailor the selection regime to generate antibodies with particular characteristics such as: high affinity, neutralization potency, ability to recognize
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"Fluorescent antibody techniques have allowed for the direct identification and enumeration of individual bacteria in environmental samples without requiring prior growth in culture media (Bahlool and Schmidt 1980, Cloete and Steyn 1988, Macario et al. 1989). The technique involves the use of specific antibodies raised against surface markers of defined pure cultures that are either labelled directly with fluorescent dye molecules or via a fluorescent secondary antibody. This approach has yielded important insights into the spatial distribution of microorganisms, but it suffers from a number of disadvantages. For example, expression of the antigen may be influenced by environmental factors; false-positive and false-negative results may be obtained due to cross-reactivity or lack of reaction; non-specific binding of antibodies may result in high levels of background fluorescence; and production of specific antibodies requires a pure culture of the organism of interest (Cloete and de Bruyn Various recombinant DNA techniques have subsequently been developed that are independent of cultivation methods (Fig. 1). These techniques provide ways of detecting and quantifying specific phylogenetic groups of microbes on 16S rDNA sequences, and relevant structural genes provide ways of monitoring microbial populations of environmental and industrial systems. In addition to these tools, a number of emerging technologies such as the use of biomarker genes are being increasingly used to monitor with great precision and accuracy the behaviour of microbes in the environment." In Recent Advances in Marine Biotechnology, Vol. 8. CRC Press, 2003. http://dx.doi.org/10.1201/9781482279986-12.

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Conference papers on the topic "Antigen-antibody reactivity"

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Salyaev, R. K., N. I. Rekoslavskaya та A. S. Stolbikov. "СHARACTERISTICS OF ANTIGEN-ANTIBODY CROSS-REACTIVITY BETWEEN DIFFERENT TYPES OF HUMAN PAPILLOMAVIRUS (HPV) DURING PLANT NUCLEAR GENETIC TRANSFORMATION". У The Second All-Russian Scientific Conference with international participation "Regulation Mechanisms of Eukariotic Cell Organelle Functions". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-318-1-114-116.

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Nordfang, O., M. Ezban, and J. B. Knudsen. "IMMUNOASSAY FOR FACTOR VIII-HEAVY CHAIN. AN INDICATOR FOR IMMUNE COMPLEXES DURING HIGH DOSE FVIII INHIBITOR TREATMENT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644718.

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Specificity studies have shown that most hemophilia A inhibitor antibodies are directed towards the light chain of coagulation factor VIII (FVIII). Thus, conventional immunoassays for FVIII-antigen (FVIII:Ag) presumably have reactivity for FVIII-Light Chain (FVIII-LC) . Our sandwich FVIII :Ag assay has been shewn to be specific for only FVIII-LC. We have now developed a specific immunoassay for FVIII-Heavy Chain (FVIII-HC) . This has made it possible to investigate the FVIII-HC content in hemophilia A plasma, and to study the expression of FVIII-HC in culture medium frcm transfected cell lines
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van der Meer, F. J. M., N. H. van Tilburg, S. R. Poort, E. Briët, and R. M. Bertina. "IMMUNOLOGICAL ASSAYS FOR THE Ca(II)-DEPENDENT AND NONCa(II)-DEPENDENT CONFORMATIONS OFJtLUMAN PROTELN Z (PZ)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643818.

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Human PZ was purified using bariumcitrate absorption and elution, DEAE-Sephadex chromatography and gelfiltration on Sephadex G-200. The purified protein was used for the development of a specific rabbit antiserum, which could be used in a electroimmunoassay (EIA). Using immunoaffinity procedures, two populations of antibodies were isolated from the antiserum: one against the Ca(II)-dependent conformation of PZ(Ca(II)PZAg) and one against the nonCa(II)-dependent conformation (nonCa(II)PZAg). These antibody populations were used for the development of specific and highly sensitive immunoradiomet
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Reports on the topic "Antigen-antibody reactivity"

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Perk, Simon, Egbert Mundt, Alexander Panshin, et al. Characterization and Control Strategies of Low Pathogenic Avian Influenza Virus H9N2. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7697117.bard.

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The avian influenza virus, subtype H9N2 subtype, defined as having a low pathogenicity, causes extensive economical losses in commercial flocks, probably due to management and synergism with other pathogens. AIV H9N2 was first identified in Israel in the year 2000, and since then it became endemic and widespread in Israel. Control by vaccination of commercial flocks with an inactivated vaccine has been introduced since 2007. In face of the continuous H9N2 outbreaks, and the application of the vaccination policy, we aimed in the present study to provide a method of differentiating naturally inf
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