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Journal articles on the topic 'Antigen-antibody reactivity'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Bentley, G. A., G. Boulot, and V. Chitarra. "Cross-reactivity in antibody-antigen interactions." Research in Immunology 145, no. 1 (1994): 45–48. http://dx.doi.org/10.1016/s0923-2494(94)80042-1.

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Bobrovnik, S. A., M. O. Demchenko, and S. V. Komisarenko. "Effect of trifluoroethanol on antibodies binding properties." Ukrainian Biochemical Journal 95, no. 1 (2023): 20–30. http://dx.doi.org/10.15407/ubj95.01.020.

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The studies on the influence of organic co-solvents on the structure and function of antibodies are of key interest, especially in view of antibodies broad use as recognizing elements in different analytical systems. Here we studied the effect of co-solvent 2,2,2-trifluoroethanol (TFE) on the ability of anti-ovalbumin monoclonal antibodies to interact with its specific antigen. Antibody affinity to antigen and the rate constants of antibody binding to immobilized antigen were analyzed. Changes in antibody reactivity with incubation time which depended on TFE concentration and temperature were

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Brüssow, H., and J. Sidoti. "Reactivity of human serum antibody with lipopolysaccharide O 78 antigen from enterotoxigenicEscherichia coli." Epidemiology and Infection 108, no. 2 (1992): 315–22. http://dx.doi.org/10.1017/s0950268800049785.

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SUMMARYFifteen and five of 20 volunteers challenged with the enterotoxigenicEscherichia colistrain O 78·H11 showed a fourfold titre increase of serum ELISA antibody to the homologous O 78 and the heterologous O 8 lipopolysaccharide antigen, respectively. Sixty-three of 191 sera from 1- to 48-month-old German children showed serum antibody reactive with O 78 antigen, all but two of these O 78-positive sera also showed reactivity with at least one further O antigen. Only 14 of the O 78 reactive sera also showed antibody to heat-labile enterotoxin. In addition, soluble O 8 antigen could inhibit t

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Thomas, C. B., D. E. Jasper, J. T. Boothby, and J. D. Dellinger. "Enzyme-linked immunosorbent assay for detection of Mycoplasma californicum-specific antibody in bovine serum: Optimization of assay determinants and control of serologic cross-reactions." American Journal of Veterinary Research 48, no. 4 (1987): 590–95. https://doi.org/10.2460/ajvr.1987.48.04.590.

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SUMMARY An enzyme-linked immunosorbent assay (elisa) was adapted to detect Mycoplasma californicum-specific antibodies in bovine serum. Cross-reactive antibody was found in the M californicum-positive reference serum when assayed against each of 7 solid-phase antigens of heterologous mycoplasma species. Cross-reactivity was further demonstrated by inhibition of elisa reactivity to M californicum solid-phase antigen by incubation of sera with antigen suspensions of each heterologous species. Incubation of test sera with a cross-reacting antigen mixture containing equal proportions of the 7 cros

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Valdarnini, Niccolò, Bettina Holm, Paul Hansen, Paolo Rovero, Gunnar Houen, and Nicole Trier. "Fine Mapping of Glutamate Decarboxylase 65 Epitopes Reveals Dependency on Hydrophobic Amino Acids for Specific Interactions." International Journal of Molecular Sciences 20, no. 12 (2019): 2909. http://dx.doi.org/10.3390/ijms20122909.

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Characterization of multiple antibody epitopes has revealed the necessity of specific groups of amino acid residues for reactivity. This applies to the majority of antibody–antigen interactions, where especially charged and hydrophilic amino acids have been reported to be essential for antibody reactivity. This study describes thorough characterization of glutamic acid decarboxylase (GAD) 65 antigenic epitopes, an immunodominant autoantigen in type 1 diabetes (T1D). As linear epitopes are sparsely described for GAD65 in T1D, we aimed to identify and thoroughly characterize two GAD65 antibodies

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Inman, R. D., B. Chiu, and N. C. Hamilton. "Analysis of immune complexes in rheumatoid arthritis for Epstein-Barr virus antigens reveals cross-reactivity of viral capsid antigen and human IgG." Journal of Immunology 138, no. 2 (1987): 407–12. http://dx.doi.org/10.4049/jimmunol.138.2.407.

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Abstract We recently defined the immunochemical characteristics of immune complexes (IC) isolated from synovial fluid (SF) of patients with rheumatoid arthritis with the use of Western blot analysis. In the present study, we probe for exogenous antigens in the IC by examining the specificity of antisera raised against the IC. Anti-IC antisera demonstrated strong reactivity against the viral capsid antigen (VCA) of Epstein Barr virus (EBV), which was not explained by preimmune reactivity, polyclonal B cell activation, or Fc-mediated binding in the immunofluorescence or ELISA systems used to mea

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Papp, Krisztián, Ágnes Kovács, Anita Orosz, et al. "Absolute Quantitation of Serum Antibody Reactivity Using the Richards Growth Model for Antigen Microspot Titration." Sensors 22, no. 10 (2022): 3962. http://dx.doi.org/10.3390/s22103962.

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In spite of its pivotal role in the characterization of humoral immunity, there is no accepted method for the absolute quantitation of antigen-specific serum antibodies. We devised a novel method to quantify polyclonal antibody reactivity, which exploits protein microspot assays and employs a novel analytical approach. Microarrays with a density series of disease-specific antigens were treated with different serum dilutions and developed for IgG and IgA binding. By fitting the binding data of both dilution series to a product of two generalized logistic functions, we obtained estimates of anti

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Larrea, Carlos Fernández de, Jacobus Henry de Waard, Francesca Giampietro, and Zaida Araujo. "The secretory immunoglobulin A response to Mycobacterium tuberculosis in a childhood population." Revista da Sociedade Brasileira de Medicina Tropical 39, no. 5 (2006): 456–61. http://dx.doi.org/10.1590/s0037-86822006000500007.

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We report on the measurement of saliva anti-Purified Protein Derivative sIgA and 38kDa antibodies from 127 children, of whom 31 were strong tuberculosis suspects and 96 were healthy contact children. The results concerning the percentage of children with antibody reactivity to PPD and 38kDa antigens showed that, of these 2 antigens, 38kDa induced higher reactivity in patients positive and negative for the Tuberculin Skin Test (28% and 16.6%, respectively) in comparison to controls positive and negative for the TST (11.7% and 7.1%, respectively). There was a statistically significant difference

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Burnett, Deborah L., Peter Schofield, David B. Langley, et al. "Conformational diversity facilitates antibody mutation trajectories and discrimination between foreign and self-antigens." Proceedings of the National Academy of Sciences 117, no. 36 (2020): 22341–50. http://dx.doi.org/10.1073/pnas.2005102117.

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Conformational diversity and self-cross-reactivity of antigens have been correlated with evasion from neutralizing antibody responses. We utilized single cell B cell sequencing, biolayer interferometry and X-ray crystallography to trace mutation selection pathways where the antibody response must resolve cross-reactivity between foreign and self-proteins bearing near-identical contact surfaces, but differing in conformational flexibility. Recurring antibody mutation trajectories mediate long-range rearrangements of framework (FW) and complementarity determining regions (CDRs) that increase bin

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Devine, Peter L., Rachel J. Quin, Paul W. Shield, Yew Wah Liew, John K. Sheehan, and David J. Thornton. "Monoclonal Antibody Recognizing a Core Epitope on Mucin." Disease Markers 14, no. 2 (1998): 99–112. http://dx.doi.org/10.1155/1998/678434.

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Monoclonal antibody TH1 (IgM) was prepared by immunizing mice with deglycosylated (TFMSA-treated) cystic fibrosis mucin. TH1 reacted strongly with TFMSA treated cystic fibrosis mucin but not with the fully glycosylated mucin, indicating reactivity with a core mucin epitope. TH1 showed no reactivity with ovine mucin (98% of glycans as sialyl-Tn) but reacted strongly with desialylated ovine mucin, indicating the epitope for this mab was the Tn-antigen (O-linked GalNAc). However, TH1 showed no reactivity with Tn-positive red blood cells, and the binding of TH1 was not inhibited by GalNAc at 2.5 m

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FAIRLIE-CLARKE, KAREN J., CHRISTINA HANSEN, JUDITH E. ALLEN, and ANDREA L. GRAHAM. "Increased exposure to Plasmodium chabaudi antigens sustains cross-reactivity and avidity of antibodies binding Nippostrongylus brasiliensis: dissecting cross-phylum cross-reactivity in a rodent model." Parasitology 142, no. 14 (2015): 1703–14. http://dx.doi.org/10.1017/s0031182015001390.

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SUMMARYMounting an antibody response capable of discriminating amongst and appropriately targeting different parasites is crucial in host defence. However, cross-reactive antibodies that recognize (bind to) multiple parasite species are well documented. We aimed to determine if a higher inoculating dose of one species, and thus exposure to larger amounts of antigen over a longer period of time, would fine-tune responses to that species and reduce cross-reactivity. Using the Plasmodium chabaudi chabaudi (Pcc)–Nippostrongylus brasiliensis (Nb) co-infection model in BALB/c mice, in which we previ

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Sibanda, Elopy N., Margo Chase-Topping, Lorraine T. Pfavayi, Mark E. J. Woolhouse, and Francisca Mutapi. "Evidence of a distinct group of Black African patients with systemic lupus erythematosus." BMJ Global Health 3, no. 5 (2018): e000697. http://dx.doi.org/10.1136/bmjgh-2017-000697.

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BackgroundThe autoimmune disease systemic lupus erythematosus (SLE) occurs more frequently in patients of African descent with high morbidity and mortality. Current SLE diagnostic criteria including antinuclear antibody (ANA) reactivity are derived largely from non-African populations. This study characterises ANA reactivity patterns and relates them to SLE clinical presentation in Black African patients.MethodsSera from Black participants (61 patients with SLE and 100 controls) aged 1–81 years were analysed for reactivity against the antigens: uridine 1-ribonuclear protein, Smith uridine-1-5

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Du, Liping, Sachiko Fukushima, Annahita Sallmyr, Rolf Manthorpe, and Anders Bredberg. "Exposure of HEp-2 Cells to Stress Conditions Influences Antinuclear Antibody Reactivity." Clinical and Vaccine Immunology 9, no. 2 (2002): 287–94. http://dx.doi.org/10.1128/cdli.9.2.287-294.2002.

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ABSTRACT This study of stress-related antinuclear antibody (ANA) reactivity was undertaken with the objective of improving clinical ANA testing. ANA was determined by parallel enzyme-linked immunosorbent assays of crude nuclear protein antigen extracted from HEp-2 cells either grown under optimal conditions (providing nonstress ANA antigen) or exposed to stress (providing stress ANA antigen). The stress stimuli used were gamma radiation (causing DNA damage) and a hypertonic environment (causing apoptosis). Signs of stress-related ANA reactivity were seen among connective tissue disease (CTD) p

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Dessau, Ram B., Linda Fryland, Peter Wilhelmsson, et al. "Study of a Cohort of 1,886 Persons To Determine Changes in Antibody Reactivity to Borrelia burgdorferi 3 Months after a Tick Bite." Clinical and Vaccine Immunology 22, no. 7 (2015): 823–27. http://dx.doi.org/10.1128/cvi.00026-15.

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ABSTRACTLyme borreliosis is a tick-borne disease caused by the bacteriumBorrelia burgdorferi. The most frequent clinical manifestation is a rash called erythema migrans. Changes in antibody reactivity toB. burgdorferi3 months after a tick bite are measured using enzyme-linked immunosorbent assays (ELISAs). One assay is based on native purified flagellum antigen (IgG), and the other assay is based on a recombinant antigen called C6 (IgG or IgM). Paired samples were taken at the time of a tick bite and 3 months later from 1,886 persons in Sweden and the Åland Islands, Finland. The seroconversion

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Berry, Vantla, Harsurinder Kaur, and Prabodh K. Sluivastava. "Studies on the Dd-Antigen-Antibody System: Antigen Dd-Reactivity in North India." Journal of Human Ecology 3, no. 4 (1992): 257–61. http://dx.doi.org/10.1080/09709274.1992.11907934.

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Gray, Julian C., Patrick H. Corran, Elena Mangia, et al. "Profiling the Antibody Immune Response against Blood Stage Malaria Vaccine Candidates." Clinical Chemistry 53, no. 7 (2007): 1244–53. http://dx.doi.org/10.1373/clinchem.2006.081695.

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Abstract Background: The complexity and diversity of the antibody immune response to the antigen repertoire of a pathogen has long been appreciated. Although it has been recognized that the detection of antibodies against multiple antigens dramatically improves the clinical sensitivity and specificity of diagnostic assays, the prognostic value of serum reactivity profiles against multiple microbial antigens in protection has not been investigated. Methods: Using malaria as a model we investigated whether antigen reactivity profiles in serum of children with different levels of clinical immunit

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Jain, Deepti, and Dinakar M. Salunke. "Antibody specificity and promiscuity." Biochemical Journal 476, no. 3 (2019): 433–47. http://dx.doi.org/10.1042/bcj20180670.

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Abstract The immune system is capable of making antibodies against anything that is foreign, yet it does not react against components of self. In that sense, a fundamental requirement of the body's immune defense is specificity. Remarkably, this ability to specifically attack foreign antigens is directed even against antigens that have not been encountered a priori by the immune system. The specificity of an antibody for the foreign antigen evolves through an iterative process of somatic mutations followed by selection. There is, however, accumulating evidence that the antibodies are often fun

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Cornaby, Caleb, and Eric T. Weimer. "Utilizing principal component analysis in the identification of clinically relevant changes in patient HLA single antigen bead solid phase testing patterns." PLOS ONE 18, no. 10 (2023): e0288743. http://dx.doi.org/10.1371/journal.pone.0288743.

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Background HLA antibody testing is essential for successful solid-organ allocation, patient monitoring post-transplant, and risk assessment for both solid-organ and hematopoietic transplant patients. Luminex solid-phase testing is the most common method for identifying HLA antibody specificities, making it one of the most complex immunoassays as each panel contains over 90 specificities for both HLA class I and HLA class II with most of the analysis being performed manually in the vendor-provided software. Principal component analysis (PCA), used in machine learning, is a feature extraction me

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Kapur, R. P., S. A. Bigler, M. Skelly, and A. M. Gown. "Anti-melanoma monoclonal antibody HMB45 identifies an oncofetal glycoconjugate associated with immature melanosomes." Journal of Histochemistry & Cytochemistry 40, no. 2 (1992): 207–12. http://dx.doi.org/10.1177/40.2.1552165.

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The anti-melanoma monoclonal antibody HMB45 is widely used in diagnostic pathology owing to its great specificity and sensitivity in identifying pigmented tumors such as malignant melanoma. However, little is known regarding the nature of the antigen(s) recognized by this antibody. In the observations reported here, the HMB45-defined antigen was identified in another pigmented tissue, the retinal pigment epithelium (RPE). A series of immunocytochemical studies demonstrated transient reactivity of the prenatal and infantile human RPE with antibody HMB45; adult RPE is non-reactive with the antib

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Wei, Dong, Guozhen Fang, and Shuo Wang. "An effective strategy for preparation of a polyclonal antibody with an addition of carbon chain of ciprofloxacin." European Journal of Inflammation 16 (January 2018): 205873921880564. http://dx.doi.org/10.1177/2058739218805645.

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In this study, we synthesized amino propyl ciprofloxacin (CPLX-NH2) as a ciprofloxacin (CPLX) derivative. Moreover, the immune antigen CPLX-NH2-BSA and coating antigen CPLX-NH2-OVA were prepared via CPLX-NH2 coupling with bovine serum albumin (BSA) and ovalbumin (OVA), respectively. Subsequently, the Kunming mice were immunized with immune antigen to obtain the polyclonal antibody with high titer. The regression equation of CPLX-NH2 antibody was y = –17.395x + 89.331 (R2 = 0.9961); IC50 and limit of detection (LOD) were 182.39 and 20.09 ng/mL, respectively. These results were superior to that

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Di Muzio, Martina, Sabrina Wildner, Sara Huber, et al. "Hydrogen/deuterium exchange memory NMR reveals structural epitopes involved in IgE cross-reactivity of allergenic lipid transfer proteins." Journal of Biological Chemistry 295, no. 51 (2020): 17398–410. http://dx.doi.org/10.1074/jbc.ra120.014243.

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Identification of antibody-binding epitopes is crucial to understand immunological mechanisms. It is of particular interest for allergenic proteins with high cross-reactivity as observed in the lipid transfer protein (LTP) syndrome, which is characterized by severe allergic reactions. Art v 3, a pollen LTP from mugwort, is frequently involved in this cross-reactivity, but no antibody-binding epitopes have been determined so far. To reveal human IgE-binding regions of Art v 3, we produced three murine high-affinity mAbs, which showed 70–90% coverage of the allergenic epitopes from mugwort polle

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Salzer, Jonatan, Maria Nyström, Göran Hallmans, Hans Stenlund, Göran Wadell, and Peter Sundström. "Epstein-Barr virus antibodies and vitamin D in prospective multiple sclerosis biobank samples." Multiple Sclerosis Journal 19, no. 12 (2013): 1587–91. http://dx.doi.org/10.1177/1352458513483888.

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Background: The antibody reactivity against Epstein-Barr nuclear antigen-1 (EBNA-1), and 25-hydroxyvitamin D (25(OH)D) status have been associated with multiple sclerosis (MS) risk. Interaction between these two factors has been proposed. Objectives: The objective of this paper is to examine the association between antibody reactivity against EBNA-1 and five EBNA-1 domains, and the risk of MS, and to examine if these antibodies and 25(OH)D status interact regarding MS risk in prospectively collected blood samples. Methods: Antibody reactivity and 25(OH)D levels were measured using ELISAs in n

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Luka, Janos, Philip M. Arlen, and Andrew Bristol. "Development of a Serum Biomarker Assay That Differentiates Tumor-Associated MUC5AC (NPC-1C ANTIGEN) from Normal MUC5AC." Journal of Biomedicine and Biotechnology 2011 (2011): 1–8. http://dx.doi.org/10.1155/2011/934757.

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A serum ELISA using a monoclonal antibody that detects a MUC5AC-related antigen (NPC-1C antigen) expressed by pancreatic and colorectal cancer was developed. The NPC-1C antibody reacts with specific epitopes expressed by tumor-associated MUC5AC that does not appear on MUC5AC from normal tissues. Based on observations of a highly specific antibody, we tested the ELISA to differentiate serum from healthy blood donors compared to serum from patients with colorectal or pancreatic cancer. Additionally, patient tumor tissue was stained to examine the expression pattern of MUC5AC-related antigen in p

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MORASSUTTI, ALESSANDRA L., LISA N. RASCOE, SUKWAN HANDALI, ALEXANDRE J. DA SILVA, PATRICIA P. WILKINS, and CARLOS GRAEFF-TEIXEIRA. "Cross-reactivity of the 31 kDa antigen of Angiostrongylus cantonensis – Dealing with the immunodiagnosis of meningoencephalitis." Parasitology 144, no. 4 (2016): 459–63. http://dx.doi.org/10.1017/s0031182016001918.

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SUMMARYThe primary causative agent of eosinophilic meningoencephalitis (EoM) in endemic regions is the nematode Angiostrongylus cantonensis. The occurrence of EoM was previously restricted to countries in Southeast Asia and the Pacific Islands; however, more recently, it has been reported from other regions, including Brazil. The commonly used diagnosis is detection of specific antibody reactivity to the 31 kDa antigen, which is derived from female worm somatic extracts. Here we report the occurrence of cross-reactivity to this antigen in sera from other parasitic infections, especially those

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Hato, T., K. Ikeda, M. Yasukawa, A. Watanabe, and Y. Kobayashi. "Exposure of platelet fibrinogen receptors by a monoclonal antibody to CD9 antigen." Blood 72, no. 1 (1988): 224–29. http://dx.doi.org/10.1182/blood.v72.1.224.224.

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Abstract We found that a monoclonal antibody to CD9 antigen, PMA2, induces fibrinogen binding to platelets and examined the mechanism for this. That PMA2 recognized the CD9 antigen was confirmed by its immunoblot- reactivity with a 24,000-dalton protein, reactivity with platelets and common acute lymphoblastic leukemia (ALL) cells, and competitive binding with the ALB6 antibody known as the CD9 antibody. At saturation, PMA2 bound to approximately 46,000 sites per platelet. The binding of 125I-fibrinogen to platelets occurred in a PMA2 concentration-dependent manner and was blocked by EDTA or a

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Hato, T., K. Ikeda, M. Yasukawa, A. Watanabe, and Y. Kobayashi. "Exposure of platelet fibrinogen receptors by a monoclonal antibody to CD9 antigen." Blood 72, no. 1 (1988): 224–29. http://dx.doi.org/10.1182/blood.v72.1.224.bloodjournal721224.

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We found that a monoclonal antibody to CD9 antigen, PMA2, induces fibrinogen binding to platelets and examined the mechanism for this. That PMA2 recognized the CD9 antigen was confirmed by its immunoblot- reactivity with a 24,000-dalton protein, reactivity with platelets and common acute lymphoblastic leukemia (ALL) cells, and competitive binding with the ALB6 antibody known as the CD9 antibody. At saturation, PMA2 bound to approximately 46,000 sites per platelet. The binding of 125I-fibrinogen to platelets occurred in a PMA2 concentration-dependent manner and was blocked by EDTA or an anti- g

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Konidaris, C., P. G. Mitlianga, and G. K. Papadopoulos. "No Specific Reactivity to E. Coli Glutamic Acid Decarboxylase from Sera of Newly-Diagnosed Insulin Dependent Diabetic Patients." International Journal of Immunopathology and Pharmacology 16, no. 2 (2003): 129–38. http://dx.doi.org/10.1177/039463200301600206.

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The 65 kD isoform of Glutamic Acid Decarboxylase (GAD), is one of the major autoantigens in human type 1 diabetes mellitus. This enzyme shares aminoacid identity, in select regions already determined as antigenic with its counterpart from E. coli. We tested the reactivity of diabetic and normal sera and an E. coli GAD-specific monoclonal antibody (2D9) to E. coli GAD by solid phase and competition ELISA, as well as immunoblotting to check for cross-reactivity of autoantibodies to the two antigens. Specific antibodies for E. coli GAD are present in diabetics and normal subjects without any diff

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Pfreundschuh, Michael, Natalie Fadle, Evi Regitz, Maria Kemele, and Klaus-Dieter Preuss. "Origin of (sporadic) Plasma Cell Myeloma: Lysolipids Are Not the Target of Clonal Immunoglobulins." Blood 128, no. 22 (2016): 2055. http://dx.doi.org/10.1182/blood.v128.22.2055.2055.

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Abstract Background: Lysolipids have been claimed to be involved in the origin of sporadic monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM) and of Gaucher's associated MGUS/MM because lyso-glucosylceramide (LGL1) and lysophosphatidylcholine (LPC) were purported to be the target of the clonal immunoglobulin in 31% of patients with sporadic and 85% with Gaucher's associated MGUS/MM(Nair et al. N Engl J Med 2016;374:555-61). The low titers (1:250) of the reported anti-lysolipid reactivity raised doubts as to whether these reactivities were indeed mediated by the

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Kadival, G. V., S. D. Chaparas, and D. Hussong. "Characterization of serologic and cell-mediated reactivity of a 38-kDa antigen isolated from Mycobacterium tuberculosis." Journal of Immunology 139, no. 7 (1987): 2447–51. http://dx.doi.org/10.4049/jimmunol.139.7.2447.

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Abstract An antigen of Mycobacterium tuberculosis with an m.w. of 38,000 has been isolated by affinity chromatography using a monoclonal antibody. This antibody bound only to an antigen found in M. tuberculosis and Mycobacterium bovis BCG. The specificity of the antigen was tested in a vertical study by immunodetection on western blots reacted with hyperimmune sera against M. tuberculosis, M. bovis, and 10 other Mycobacterium species. The antigen was detected only by antisera to M. tuberculosis and M. bovis. Specificity in cell-mediated immunity was tested by skin tests in guinea pigs sensitiz

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Suzuki, T., S. K. Sanders, J. L. Butler, G. L. Gartland, K. Komiyama, and M. D. Cooper. "Identification of an early activation antigen (Bac-1) on human B cells." Journal of Immunology 137, no. 4 (1986): 1208–13. http://dx.doi.org/10.4049/jimmunol.137.4.1208.

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Abstract We have produced a monoclonal antibody, Bac-1, that appears to identify a novel antigen on activated human B cells. The Bac-1 antigen can be detected between 8 to 16 hr, as well as transferrin receptors (T9), after activation of small resting B cells with phorbol myristic acetate, anti-IgM antibody, Staphylococcus aureus Cowan I, or Epstein-Barr virus. The expression of the Bac-1 antigen precedes that of IL 2 receptors (Tac-1). Peak expression of the Bac-1 antigen was observed on day 3 after activation, and decreased thereafter. The Bac-1 antigen was present on a minor subpopulation o

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Viac, J., M. Haftek, MJ Staquet, A. Reano, J. Brochier, and J. Thivolet. "A monoclonal antibody labelling the keratinocyte membrane: a marker of epidermal differentiation." Acta Dermato-Venereologica 65, no. 1 (1985): 1–8. http://dx.doi.org/10.2340/000155556518.

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A murine hybridoma secreting an IgM monoclonal antibody (KL3) was produced by cell fusion of mouse myeloma cells with spleen cells from mice immunized with human epidermal keratins. On normal human epidermis KL3 stained the intercellular spaces from the stratum germinatum to the stratum granulosum with a fluorescence intensity increasing from the basal layer to the upper layers. Basal cells were not stained on the side facing the basement membrane. About 90% of free keratinocytes isolated after trypsinization were labelled by KL3 in a punctate staining. Immunoelectron microscopy allowed us to

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Kirchenbaum, Greg A., Graham Pawelec, and Paul V. Lehmann. "The Importance of Monitoring Antigen-Specific Memory B Cells, and How ImmunoSpot Assays Are Suitable for This Task." Cells 14, no. 3 (2025): 223. https://doi.org/10.3390/cells14030223.

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Determining an individual’s humoral immune reactivity to a pathogen, autoantigen, or environmental agent is traditionally accomplished through the assessment of specific antibody levels in blood. However, in many instances, titers of specific antibodies decline over time and thus do not faithfully reveal prior antigen exposure or establishment of immunological memory. To estimate an individual’s humoral immune competence, it is therefore necessary to assess functional B cell memory. Here, we describe novel B cell ELISPOT and FluoroSpot assays (collectively referred to as ImmunoSpot) that can b

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Sundström, P., L. Nyström, E. Jidell, and G. Hallmans. "EBNA-1 reactivity and HLA DRB1*1501 as statistically independent risk factors for multiple sclerosis: a case-control study." Multiple Sclerosis Journal 14, no. 8 (2008): 1120–22. http://dx.doi.org/10.1177/1352458508092353.

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Objectives and methods The interaction between the two best documented risk factors (human leukocyte antigen [HLA] class II [DRB1*1501 positivity] and Epstein-Barr virus [elevated Epstein-Barr nuclear antigen 1 (EBNA-1) antibody reactivity]) for multiple sclerosis (MS) was studied in a case-control study of biobank samples from 109 MS cases and 212 matched referents. Results Multivariate logistic regression analysis showed that both were statistically significant in both sexes. HLA DRB1*1501-positive referents had higher EBNA-1 reactivity than HLA-negative referents. Less EBNA-1 reactivity was

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May, Kenneth F., Bettina Franz, Christopher Harvey, F. Stephen Hodi, Glenn Dranoff, and Kai Wucherpfennig. "Isolation of human anti-MICA antibody from cancer patients responding to immunotherapies." Journal of Clinical Oncology 30, no. 15_suppl (2012): 2502. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.2502.

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2502 Background: Immunotherapy is a promising modality for the treatment of cancer, and elucidating the mechanism of action is crucial to guiding patient selection and developing future immunotherapeutic strategies. Engagement of the immune molecule NKG2D by the cancer antigen MICA is important for immune surveillance of many cancers. Tumors can evade immune surveillance by shedding cell surface MICA, which leads to dampening of anti-tumor immunity. Investigations by our laboratory revealed that patients responding to immunotherapy can mount antibody responses targeting MICA, which permits re-

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McGill, Svena L., Russell L. Regnery, and Kevin L. Karem. "Characterization of Human Immunoglobulin (Ig) Isotype and IgG Subclass Response to Bartonella henselae Infection." Infection and Immunity 66, no. 12 (1998): 5915–20. http://dx.doi.org/10.1128/iai.66.12.5915-5920.1998.

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ABSTRACT Serologic parameters of cat scratch disease (CSD) were evaluated by Western blot analysis. Sera from patients with serologically confirmed CSD antigen were screened for immunoglobulin (Ig) isotype-specific as well as IgG subclass-specific reactivity against Bartonella henselae whole-cell antigen. Bartonella-negative control sera were used to determine baseline antibody activity. Heterogeneous B. henselae-specific IgG reactivity with numerous protein bands, ranging from >150 to <17 kDa, was observed. Though individual banding patterns were variable, one approximately 83-kDa B. he

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Sandoval, C., and B. Vásquez. "Evaluation of Immunohistochemical Reactivity with Avidin-Biotin Complex (ABC) Method." International Journal of Medical and Surgical Sciences 3, no. 3 (2018): 909–18. http://dx.doi.org/10.32457/ijmss.2016.024.

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Immunohistochemistry is any technique that can detect cellular and extracellular components in situ by means of specific antibodies, using enzymatic detection systems. Among immunohistochemical methods, the technique of avidin - biotin complex (ABC) is widely used because of its high sensitivity. The aim of this study was to evaluate the immunohistochemical reactivity of the 4C4.9 antibody for detection of S-100 protein using the ABC method. For the evaluation of immunohistochemical reactivity 2 biopsies of human skin were used with histopathological diagnosis of ulcerated malignant melanoma a

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Ichihara, Aina, Hinako Ojima, Kazuyoshi Gotoh, et al. "Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection." Toxins 13, no. 7 (2021): 467. http://dx.doi.org/10.3390/toxins13070467.

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The infection caused by Helicobacter pylori is associated with several diseases, including gastric cancer. Several methods for the diagnosis of H. pylori infection exist, including endoscopy, the urea breath test, and the fecal antigen test, which is the serum antibody titer test that is often used since it is a simple and highly sensitive test. In this context, this study aims to find the association between different antibody reactivities and the organization of bacterial genomes. Next-generation sequences were performed to determine the genome sequences of four strains of antigens with diff

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Koehler, Megan A., Lusheng Song, Francisca J. Grill, et al. "Discovery of a Unique Set of Dog-Seroreactive Coccidioides Proteins Using Nucleic Acid Programmable Protein Array." Journal of Fungi 10, no. 5 (2024): 307. http://dx.doi.org/10.3390/jof10050307.

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Valley Fever (VF), caused by fungi in the genus Coccidioides, is a prevalent disease in southwestern and western parts of the United States that affects both humans and animals, such as dogs. Although the immune responses to infection with Coccidioides spp. are not fully characterized, antibody-detection assays are used in conjunction with clinical presentation and radiologic findings to aid in the diagnosis of VF. These assays often use Complement Fixation (CF) and Tube Precipitin (TP) antigens as the main targets of IgG and IgM reactivity, respectively. Our group previously reported evidence

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Saccani Jotti, G., M. Fontanesi, A. Colombo, M. Valtolina, and A. Tardini. "Localization of Mucinous - like Carcinoma Associated Antigen (MCA) in Breast Pathology: Comparison with Carcinoembryonic Antigen (CEA) and Tissue Polypeptide Antigen (TPA)." International Journal of Biological Markers 5, no. 3 (1990): 145–52. http://dx.doi.org/10.1177/172460089000500308.

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The topographic distribution of a mucinous-like cancer antigen (MCA) recognized by a monoclonal antibody b-12 (MAb b-12) was assessed in benign (38) and malignant (66) breast tissues. The reactivity of MAb b-12 showed a good selectivity for breast tissues, reacting both with normal tissues and breast cancer. The degree of MCA expression was evaluated in the various groups of breast pathology adopting quantitative criteria of assessment. With the criteria of evaluation adopted, strong staining was observed in 71.4% breast carcinomas. The most positive reaction was demonstrated in mucinous carci

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Andrews, Gordon A., Patricia S. Chavey, and Joseph E. Smith. "Production, characterization, and applications of a murine monoclonal antibody to dog erythrocyte antigen 1.1." Journal of the American Veterinary Medical Association 201, no. 10 (1992): 1549–52. http://dx.doi.org/10.2460/javma.1992.201.10.1549.

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Summary A murine IgM monoclonal antibody, which recognizes dog erythrocyte antigen (dea) 1.1, has been produced. The antibody correctly identified canine rbc possessing dea 1.1 in a panel of rbc typed by an independent laboratory. Reactivity of the monoclonal antibody was compared with canine anti-dea 1.1 antiserum with 163 rbc samples from 145 dogs. Results of agglutination tests with the 2 reagents were in agreement for all samples. A card agglutination test that uses the monoclonal antibody with blood is described. A monoclonal antibody-based test should facilitate blood typing for dea 1.1

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Cohavy, Offer, Gunter Harth, Marcus Horwitz, et al. "Identification of a Novel Mycobacterial Histone H1 Homologue (HupB) as an Antigenic Target of pANCA Monoclonal Antibody and Serum Immunoglobulin A from Patients with Crohn's Disease." Infection and Immunity 67, no. 12 (1999): 6510–17. http://dx.doi.org/10.1128/iai.67.12.6510-6517.1999.

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ABSTRACT pANCA is a marker antibody associated with inflammatory bowel disease (IBD), including most patients with ulcerative colitis and a subset with Crohn's disease. This study addressed the hypothesis that pANCA reacts with an antigen(s) of microbial agents potentially relevant to IBD pathogenesis. Using a pANCA monoclonal antibody, we have previously identified the C-terminal basic random-coil domain of histone H1 as a pANCA autoantigen. BLAST analysis of the peptide databases revealed H1 epitope homologues in open reading frames of theMycobacterium tuberculosis genome. Western analysis o

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Imam, S. A., E. F. Esteban, L. L. Young, and C. R. Taylor. "Generation of a murine monoclonal antibody to normal mammary epithelium using mice rendered immune-tolerant to malignant mammary epithelium." Journal of Histochemistry & Cytochemistry 42, no. 5 (1994): 585–91. http://dx.doi.org/10.1177/42.5.7512585.

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A monoclonal antibody (MAb) that distinguishes normal from malignant mammary epithelia in tissue or cell lines was generated using a procedure that involved immune-tolerization before immunization. Immune-tolerance to two transformed mammary epithelial cell lines (MCF.7 and MDA.MB.231 cell lines combined) was induced in neonatal mice within 24 hr of birth. Successful induction of immune-tolerance was determined by an indirect immunohistological method, testing sera from mice against the tolerogen (i.e., the MCF.7 and MDA.MB.231 cell lines). Mice lacking antibodies in their sera against the imm

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Okayama, A., B. Korber, YM Chen, et al. "Unusual pattern of antibodies to human T-cell leukemia virus type-I in family members of adult T-cell leukemia patients." Blood 78, no. 12 (1991): 3323–29. http://dx.doi.org/10.1182/blood.v78.12.3323.3323.

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Abstract Detection methods for the human T-cell leukemia virus type-I (HTLV-I) for blood screening and diagnosis generally rely on antibody tests that use the structural proteins of HTLV-I as antigen. We have found an unusual pattern of antibody reactivity among people who are at high risk of HTLV infection due to being a family member of an adult T-cell leukemia (ATL) patient: a specific antibody reaction exclusively directed to the HTLV regulatory protein tax, and not to the HTLV-I structural proteins. Sera from 7 of 82 (8.5%) structural antibody- undetectable family members of ATL patients

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Okayama, A., B. Korber, YM Chen, et al. "Unusual pattern of antibodies to human T-cell leukemia virus type-I in family members of adult T-cell leukemia patients." Blood 78, no. 12 (1991): 3323–29. http://dx.doi.org/10.1182/blood.v78.12.3323.bloodjournal78123323.

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Detection methods for the human T-cell leukemia virus type-I (HTLV-I) for blood screening and diagnosis generally rely on antibody tests that use the structural proteins of HTLV-I as antigen. We have found an unusual pattern of antibody reactivity among people who are at high risk of HTLV infection due to being a family member of an adult T-cell leukemia (ATL) patient: a specific antibody reaction exclusively directed to the HTLV regulatory protein tax, and not to the HTLV-I structural proteins. Sera from 7 of 82 (8.5%) structural antibody- undetectable family members of ATL patients had the a

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Di, Cristina Manlio, Luisa Nunziangeli, MariaAngela Giubilei, et al. "An antigen microarray immunoassay for multiplex screening of mouse monoclonal antibodies." Nature protocols 5, no. 12 (2010): 1932. https://doi.org/10.1038/nprot.2010.161.

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The mouse monoclonal antibody (mAb) technology still represents a key source of reagents for research and clinical diagnosis, although it is relatively inefficient and expensive and therefore unsuitable for high-throughput production against a vast repertoire of antigens. In this article, we describe a protocol that combines the immunization of individual mice with complex mixtures of influenza virus strains and a microarray-based immunoassay procedure to perform a parallel screening against the viral antigens. The protocol involves testing the supernatants of somatic cell hybrids against a ca

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Rhodin, Nikki R., Marloes L. J. A. Van Tilburg, Monika W. Oli, William P. McArthur, and L. Jeannine Brady. "Further Characterization of Immunomodulation by a Monoclonal Antibody against Streptococcus mutans Antigen P1." Infection and Immunity 72, no. 1 (2004): 13–21. http://dx.doi.org/10.1128/iai.72.1.13-21.2004.

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ABSTRACT We demonstrated previously that mucosal immunization of mice with Streptococcus mutans coated with the monoclonal antibody (MAb) 6-11A directed against the major surface adhesin protein P1 results in changes in the amount, isotype distribution, and specificity of serum antibodies compared with animals immunized with bacteria only. We now show that the specificity of the mucosal secretory IgA response was also influenced by this MAb. Changes in antibody specificity were associated with changes in biological activity. Serum samples which differed in antibody reactivity with P1 polypepti

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Kubickova, Barbara, Jörg A. Schenk, Franziska Ramm, et al. "A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen." Applied Microbiology and Biotechnology 105, no. 12 (2021): 4957–73. http://dx.doi.org/10.1007/s00253-021-11342-7.

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Abstract To generate a hepatitis E virus (HEV) genotype 3 (HEV-3)–specific monoclonal antibody (mAb), the Escherichia coli–expressed carboxy-terminal part of its capsid protein was used to immunise BALB/c mice. The immunisation resulted in the induction of HEV-specific antibodies of high titre. The mAb G117-AA4 of IgG1 isotype was obtained showing a strong reactivity with the homologous E. coli, but also yeast-expressed capsid protein of HEV-3. The mAb strongly cross-reacted with ratHEV capsid protein derivatives produced in both expression systems and weaker with an E. coli–expressed batHEV c

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Abdelmonem, M., N. Ngo, M. Cabungan, et al. "Evaluation of Anti-k Antibody in a Patient Undergoing Redo Sternotomy." American Journal of Clinical Pathology 162, Supplement_1 (2024): S146. http://dx.doi.org/10.1093/ajcp/aqae129.324.

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Abstract Introduction/Objective Introduction: The Kell blood group system, discovered in 1946, holds paramount significance in transfusion medicine and hemolytic disease of the newborn (HDN). Named after Mrs. Kelleher, who exhibited anti-Kell antibodies resulting in HDN, this system comprises 25 antigens, with the original K antigen retaining primary importance. Additionally, Levine et al. identified anti-Cellano, now known as anti-k, presenting an antibody antithetical to anti-K. Methods/Case Report Case Presentation: A 60-year-old male with a history of bioprosthetic aortic valve insufficien

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van Wijland, Michel J. A., J. Henriëtte Klinkspoor, Laurens Th de Wit, Ronald P. J. Oude Elferink, Guido N. J. Tytgat, and Albert K. Groen. "Heterogeneity of Human Gallbladder Mucin in Bile." Clinical Science 86, no. 1 (1994): 67–74. http://dx.doi.org/10.1042/cs0860067.

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1. Human gallbladder mucin has been implicated to play a role in gallstone disease. In spite of this fact relatively little is known about the structure of human gallbladder mucin. In this study we have investigated the possible heterogeneity of mucin. For this purpose polyclonal and monoclonal antibodies against gallbladder mucin were raised. All antibodies reacted primarily with carbohydrate antigenic determinants. With these antibodies the immunoreactivity of gallbladder mucin from 60 patients with cholesterol gallstones and 20 subjects without stones was screened. In addition, reactivity w

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Reyes, Raphael, Louise Turner, Isaac Ssewanyana, et al. "91074 Identification of monoclonal antibodies with broad reactivity against the malaria parasite variant surface antigen responsible for severe malaria." Journal of Clinical and Translational Science 5, s1 (2021): 18–19. http://dx.doi.org/10.1017/cts.2021.451.

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ABSTRACT IMPACT: This study aims to provide insight into naturally acquired immunity against severe malaria, thereby laying the foundation for the design of novel vaccine candidates to prevent severe disease as well as monoclonal antibody therapies to treat severe malaria. OBJECTIVES/GOALS: Severe malaria is caused by parasite surface antigens that contain high sequence diversity. Nevertheless, P. falciparum-exposed individuals develop antibody responses against these antigens. Our goal is to isolate antibodies with broad reactivity to understand how disease protection is acquired. METHODS/STU

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