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Dissertations / Theses on the topic 'Antimicrobial testing'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Martin, Siseko. "In Vitro antimicrobial synergy testing of Acinetobachter Baumannii." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5228.

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Bibliography<br>Thesis (MMed (Pathology. Medical Microbiology))--University of Stellenbosch, 2011.<br>ENGLISH ABSTRACT: Acinetobacter baumannii has emerged as one of the most troublesome nosocomial pathogens globally. This organism causes infections that are often extremely difficult to treat because of the widespread resistance to the major antibiotic groups. Colonization or infection with multidrugresistant A. baumannii is associated with the following risk factors: prolonged hospital stay, admission to an intensive care unit (ICU), mechanical ventilation, and exposure to broad spectrum

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Brady, Aaron John. "Rapid colorimetric based antimicrobial susceptibility testing :development, evaluation and potential." Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.492332.

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Rapid and reliable antimicrobial susceptibility testing plays a vital role in deciding the appropriate antibiotic therapy prescribed. Current techniques employed by clinical laboratories include breakpoint tests and microdilution assays which typically provide susceptibility data after 18 to 24 hours. The primary aim of this study was to develop a reliable antimicrobial susceptibility test, using a tetrazolium-based salt as a colorimetric indicator ofbacterial metabolism, which could provide accurate susceptibility data within 4 to 5 hours. Using clinical isolates from several bacterial specie

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Kapel, Natalia. "Rapid species identification and antimicrobial susceptibility testing using Raman spectroscopy." Thesis, Swansea University, 2013. https://cronfa.swan.ac.uk/Record/cronfa42219.

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Infectious diseases remain a serious threat to human life and health as well as having important economical factor. One way of successful combating diseases is designing the most appropriate treatment plan following the correct diagnosis. Therefore, there is a need for a method combining reproducibility, precision and speed. The aim of this work was to evaluate the potential of micro-Raman spectroscopy for identifying bacteria at different taxonomic levels, strains revealing different antibiotic resistance profiles, and for phylogenetic investigation. The project was based on a selection of ba

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Lu, Yi. "A Hybrid Electrokinetic Bioprocessor For Single-Cell Antimicrobial Susceptibility Testing." Diss., The University of Arizona, 2015. http://hdl.handle.net/10150/579108.

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Infectious diseases resulting from bacterial pathogens are the most common causes of patient morbidity and mortality worldwide. The rapid identification of the pathogens and their antibiotic resistances is crucial for proper clinical management. However, the standard culture-based diagnostic approach requires a minimum of two days from the initial specimen collection to result reporting. As a consequence, broad-spectrum antibiotics are often prescribed under the worst-case assumption without knowledge of the pathogens or their resistances. The current clinical practice results in improper trea

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Morgan, Marcella Alexandra. "Antimicrobial resistance in Staphylococcus aureas." Scholarly Commons, 1988. https://scholarlycommons.pacific.edu/uop_etds/2147.

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Susceptibility of 112 strains of Staphylococcus aureus obtained from Dameron Hospital, Stockton, California was tested with 18 antimicrobials . The MIC method was used with the following antimicrobials : tetracycline, oxacillin, penicillin, ampicillin, vancomycin, cefazolin, erythromycin, clindamycin, gentamycin, rifampin, trimethoprimsulfamethoxazole, chloramphenicol, and cefotaxime . The standard Kirby-Bauer disc diffusion method was used to test neomycin, tobramycin, and amikacin . Methicillin, oxacillin, and nafcillin were tested with a modified Kirby-Bauer method, which included the addit

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Miller, Ron Ashley. "Development of standardized antimicrobial susceptibility testing methods and aeromonas salmonicida epidemiologic cutoff values for antimicrobial agents used in aquaculture." College Park, Md. : University of Maryland, 2007. http://hdl.handle.net/1903/7256.

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Thesis (Ph. D.) -- University of Maryland, College Park, 2007.<br>Thesis research directed by: Marine-Estuarine-Environmental Sciences. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.

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Wicht, Merrill Margaret. "Oleanolic acid: its isolation and derivatisation to potential antimicrobial compounds." Thesis, Cape Peninsula University of Technology, 2007. http://hdl.handle.net/20.500.11838/738.

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Thesis (MTech (Chemistry))--Cape Peninsula University of Technology, 2007<br>An increasing number of natural products possessing the oleanolic acid moiety have been shown to demonstrate a wide spectrum of biological activity. This thesis deals with the extraction and isolation of oleanolic acid from Syzigium aromaticum and the examination of its stereochemistry and crystal structure by X-ray diffraction. The synthetic routes used for converting functional groups on the oleanolic acid molecule to afford derivatives are described in Chapter 5. Oleanolic acid and its derivatives were evalu

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Wicht, Merril Margaret. "Oleanic acid: its isolation and derivatisation to potential antimicrobial compounds." Thesis, Cape Peninsula University of Technology, 2007. http://hdl.handle.net/20.500.11838/732.

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A thesis submitted to the Cape Peninsula University of Technology in fulfilment of the requirements for the MASTERS DEGREE IN TECHNOLOGY (CHEMISTRY) Department of Chemistry January 2007<br>An increasing number of natural products possessing the oleanolic acid moiety have been shown to demonstrate a wide spectrum of biological activity. This thesis deals with the extraction and isolation of oleanolic acid from Syzigium aromaticum and the examination of its stereochemistry and crystal structure by X-ray diffraction. The synthetic routes used for converting functional groups on the oleanolic acid

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Duckworth, Peter Francis. "Chlorhexidine-phosphate salts in wound care : the development and testing of antimicrobial functionalised materials." Thesis, University of Bristol, 2017. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.738245.

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Sundberg, Kristin L. "Application of Materials Characterization, Efficacy Testing, and Modelling Methods on Copper Cold Spray Coatings for Optimized Antimicrobial Properties." Digital WPI, 2019. https://digitalcommons.wpi.edu/etd-dissertations/511.

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The Copper Development Association (CDA) has identified over 450 copper alloys registered with the U.S. Environmental Protection Agency (EPA) as antimicrobial. With growing antibiotic resistance, there is a need for copper coatings with increased antimicrobial capability. Cold spray is a high velocity, high deposition rate process that forms dense coatings with little to no oxides or inclusions. It is possible that this process contributes to the increased antimicrobial capability of copper cold spray coatings as compared to other additive processes. The focus of this effort is to understand

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11

Tassev, Dimiter V. "Antimicrobial susceptibility testing of novel anticancer derivatives against infectious bacteria for the potential minimization of nosocomial infections." Connect to resource, 2006. http://hdl.handle.net/1811/6457.

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Thesis (Honors)--Ohio State University, 2006.<br>Title from first page of PDF file. Document formatted into pages: contains 27 p.; also includes graphics. Includes bibliographical references (p. 24-27). Available online via Ohio State University's Knowledge Bank.

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Harris, Mark R. "Effects of cationic antimicrobial peptides on Candida and Saccharomyces species." Thesis, St Andrews, 2010. http://hdl.handle.net/10023/881.

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13

Patel, Nehal Jitendralal. "Comparison of Antibiotic Sensitivity Profiles, Molecular Typing Patterns, and Attribution of Salmonella Enterica Serotype Newport in the U.S., 2003-2006." Digital Archive @ GSU, 2007. http://digitalarchive.gsu.edu/iph_theses/12.

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Salmonella causes gastrointestinal illness in humans. The purpose of the study was to determine the relative contribution of different food commodities to sporadic cases of salmonellosis (attribution analysis) caused by Salmonella Newport (SN) using Pulsed-Field Gel Electrophoresis (PFGE) patterns and antimicrobial sensitivity (AST) data submitted by public health laboratories and regulatory agencies from 2003 to 2006. The genetic relationship between isolates from non-human (348) and human (10,848) sources was studied by two unique clustering methods: UPGMA and Ward. Results show poultry wa

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Olafsson, Jonas. "Antimicrobial Susceptibility Testing Directly from Urine Samples : a Comparison between Standardised and Direct Disk Diffusion Testing together with Direct Species Identification using Matrix Assisted Laser Desorption/Ionisation Time of Flight." Thesis, Linnéuniversitetet, Institutionen för naturvetenskap, NV, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-27645.

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Urinary tract infection (UTI) is a very common infection in humans and a majority is caused by Escherichia coli. UTI are commonly treated empirically. However, empiric treatment has become more problematic due to increased antibiotic resistance to commonly used antibiotic agents. It is therefore desirable with short turnover times for antimicrobial susceptibility testing and species identification to improve antibiotic treatment at an early stage. Matrix Assisted Laser Desorption/Ionisation Time of Flight (MALDI-TOF) can provide species identification faster than former routine methods. This s

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Momin, Vasim. "The analysis of antimicrobial testing Vincetoxicum stocksii and isolation of a highly active compound against Candida albicans by using various different techniques." unrestricted, 2008. http://etd.gsu.edu/theses/available/etd-04242008-132516/.

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Thesis (M.S.)--Georgia State University, 2008.<br>Title from file title page. Keith Pascoe, committee chair; Alfons Baumstark, Jerry Smith, Davon Kennedy, committee members. Electronic text (46 p. : ill. (some col.)) : digital, PDF file. Description based on contents viewed July 8, 2008. Includes bibliographical references (p. 45-46).

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Momin, Vasim Rasul. "The Analysis of Antimicrobial Testing Vincetoxicum stocksii and Isolation of a Highly Active Compound Against Candida albicans by Using Various Different Techniques." Digital Archive @ GSU, 2008. http://digitalarchive.gsu.edu/chemistry_theses/9.

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The purpose is to isolate a natural compound, which shows a high activity against Candida albicans, from plant, Vincetoxicum stocksii. Bio-Assay, Thin layer Chromatography, Column Chromatography, TLC bio Assay, and other extraction techniques are used in order to isolate the active compound. First, bio assay technique is carried out on the crude gum. Next, several flash chromatography columns are carried out in order to isolate the target compound, which has a Rf value of ~0.53 in 10:1 DCM/methanol solvent mixture. The TLC bioassay technique is also carried out in order to confirm the hyp

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Sousa, Bruno Abdon Inácio de. "Avaliação toxicológica de misturas dos medicamentos veterinários (Monensina, Sulfametazina e Enrofloxacina) em Daphnia magna (Cladocera, Crustacea)." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/64/64135/tde-28012014-160019/.

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Esse trabalho teve como objetivo o estudo da toxicidade aguda e crônica da ação isolada e de misturas binárias de três medicamentos veterinários (Monensina, Sulfametazina e Enrofloxacina) para o organismo teste Daphnia magna. A toxicidade aguda da enrofloxacina determinada foi de CE50 - 54.36 mgL-1, da monensina CE50 - 15.11mgL-1 e da sulfametazina CE50 - 183.80 mgL-1. Para os ensaios de toxicidade crônica foram determinados 3 \"endpoints\" (sobrevivência, reprodução e tamanho do adulto) e foi determinado o CEO para a enrofloxacina de 0,33 mgL-1, da monensina 0,09 mgL-1 e da sulfametazina de 6

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Scheuring, Toni. "Implementing Usability Engineering into Development of an Innovative Antibiotic Susceptibility Testing Device." Thesis, Uppsala universitet, Industriell teknik, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-396589.

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During the last decades, newly developed medical devices often came along with unappropriate designs, increasing the likelihood of misuse through the operator. Part of the root cause was that no sufficient measures were applied to assimilate user needs. Consequently, usability engineering approaches are now stronger emphasized to ensure that new devices are not only safe to use but are also designed for users’ needs. Besides, testing processes in clinical microbiology laboratories are currently reshaped due to new generations of rapid testing methods. Hence, it is particularly important to app

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IDREES, AYESHA. "Development of 3D skin model and 3D skin infection model, as advanced testing tools for the bio-evaluation of antimicrobial biomaterials for wound healing." Doctoral thesis, Politecnico di Torino, 2019. http://hdl.handle.net/11583/2743229.

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Ray, Lucille Alexandria. "Live single cell fluorescence microscopy; from antibiotic resistance detection to mitochondrial dysfunction." University of Akron / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=akron1597342775751888.

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Hahlin, Emma. "Optimering av metod för upparbetning av Klebsiella pneumoniae från blododlingskultur inför flödescytometriassisterad resistensbestämning." Thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-85895.

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Under vissa omständigheter kan bakterier kan ta sig in i blodbanan där de kan orsaka allvarliga infektioner (bakteriemi). Metoden som används för identifiering och resistensbestämning av bakterier i blododlingar kräver minst 16 timmars inkubation. Fram tills en resistensbestämning utförts kan empirisk antibiotikabehandling användas, men med ökande resistensutbredning blir det alltmer osäkert om denna behandling är verksam. Nyligen har en lappdiffusionsmetod för resistensbestämning direkt från blododling validerats, som kan läsas av efter 4, 6 och/eller 8 timmars inkubation. Det finns även publ

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Westman, Natalee. "Direktresistensbestämning för blododlingar : Volymoptimering och reproducerbarhet." Thesis, Jönköping University, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-53082.

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Direct antimicrobial susceptibility testing (dAST) is not a standardized method and there is no recommendations regarding the volume of blood culture media to be used. The aim of the study was to optimize the method of dAST by determining a volume of blood culture media in µL to be used and to test the reproducibility of the volume optimized method. The dASTs (n=160) were performed on blood culture bottles (n=40) inoculated with reference bacteria (n=4), using four test volumes (50 µL, 75 µL, 100 µL and 125 µL). The optimized method was implemented on frozen and fresh bacterial isolates (n=120

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Kulengowski, Brandon. "CARBAPENEM-RESISTANT ENTEROBACTERIACEAE: EPIDEMIOLOGY, GENETICS, IN VITRO ACTIVITY, AND PHARMACODYNAMIC MODELING." UKnowledge, 2019. https://uknowledge.uky.edu/pharmacy_etds/96.

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Background: Infections caused by carbapenem-resistant Enterobacteriaceae (CRE) such as Escherichia coli and Klebsiella pneumoniae are among the most urgent threats of the infectious disease realm. The incidence of these infections has been increasing over the years and due to very limited treatment options, mortality is estimated at about 50%. By 2050, mortality from antimicrobial resistant infections is expected to surpass cancer at 10 million deaths annually. Methods: We evaluated 18 contemporary antimicrobials against 122 carbapenem-resistant Enterobacteriaceae using a variety of antimicrob

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Feliciano, Cinara Silva. "Avaliação da resistência de Mycobacterium tuberculosis a drogas através de testes fenotípicos, moleculares comerciais e do sequenciamento genômico total." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/17/17138/tde-23072018-100514/.

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A tuberculose (TB) embora passível de tratamento efetivo, ainda é um grave problema de saúde pública em diversos países, inclusive no Brasil. Nas últimas décadas houve progressos consistentes no controle da doença, porém o avanço da resistência bacilar ainda é um desafio a ser superado, já que os mecanismos da resistência são bastante complexos e não totalmente conhecidos, o que dificulta o desenvolvimento de testes de sensibilidade com elevada acurácia. O objetivo deste trabalho foi caracterizar as mutações gênicas de cepas de Mycobacterium tuberculosis de pacientes do Brasil e de Moçambique

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Aziz, Seemal. "Antibiotic Susceptibility Testing: Effects Of Variability In Technical Factors On Minimum Inhibitory Concentration Using Broth Microdilution." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-454819.

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Background Broth microdilution (BMD) is a gold-standard reference method to determine minimum inhibitory concentration (MIC) of antibiotics. For this, a standardized concentration of bacterial inoculum (2e5–8e5 colony-forming units, CFU/ml) is added to progressively higher concentrations of antibiotics. Bacteria stop growing at a particular antibiotic concentration termed MIC. Like other assays, various biological and/or technical factors can affect BMD results. Aims To investigate the effects of inoculum concentration (5e4–5e6 CFU/ml), growth-medium concentration (cation-adjusted Mueller-Hi

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Fiscon, Marta. "Validazione di un percorso diagnostico rapido per l'analisi del fenotipo di sensibilità dei batteri Gram negativi." Doctoral thesis, Università degli studi di Padova, 2017. http://hdl.handle.net/11577/3426658.

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The multidrug-resistant bacterial infections represent a global and complex problem because of the increase in their prevalence and the lack of new antibiotics. In this context, the antimicrobial stewardship programs are based on the collaboration between the infectious disease specialist and the microbiologist, which must merge their competencies to optimize antimicrobial therapy, implement surveillance and control the spread of MDR epidemic in health-care facilities. Moreover, they should create specific diagnostic procedures to obtain a prompt diagnosis of sepsis, with the species-identific

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Bengtsson, Moa, and Hanna Jacobsson. "Verifiering av mikrobuljongspädning med Sensititre™-paneler för MIC-bestämning av grampositiva kocker." Thesis, Jönköping University, HHJ, Avd. för naturvetenskap och biomedicin, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-52931.

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Resistensbestämningar som genererar ett kvantitativt MIC-värde är värdefullt för att kunna välja adekvat antibiotikabehandling. Referensmetoden för MIC-bestämning är mikrobuljongspädning där kommersiella antibiotikapaneler underlättar handhavandet av metoden på kliniska laboratorier. Syftet med studien var att verifiera mikrobuljongspädning med Sensititre™-panelerna SEMSE7 och SEMST7 avsedda för grampositiva kocker, genom att jämföra erhållet resultat med tidigare uppmätta referensvärden av MIC samt SIR-kategorier. Mikrobuljongspädning utfördes med SEMSE7-panel och MH-buljong för Staphylococcu

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MARTINS, Marlene Andrade. "Avaliação de feridas crônicas em pacientes atendidos em Unidades Básicas de Saúde de Goiânia." Universidade Federal de Goiás, 2008. http://repositorio.bc.ufg.br/tede/handle/tde/698.

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Made available in DSpace on 2014-07-29T15:04:30Z (GMT). No. of bitstreams: 1 dissertacao marleneandrade.pdf: 776220 bytes, checksum: 89f7e969fbef687591092b3ded9dd40a (MD5) Previous issue date: 2008-04-30<br>Assessing patients with chronic wounds poses a challenge to professionals and doubts still persist concerning characterization of the wound infection status. We believe that the Basic Health Units are in places of reference for the public to submit chronic wounds and should have full attendance and resolution were objectives of this study: characterizing patients with chronic wounds atten

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Echeverrigaray, Fernando Graniero. "Estudo da ação antimicrobiana pela modificação de regiões próximas à superfície de aço inoxidável." reponame:Repositório Institucional da UCS, 2014. https://repositorio.ucs.br/handle/11338/845.

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Os biofilmes constituem uma nova forma de vida que oferece maior resistência aos micro-organismos, protegendo-os contra agressões físicas e químicas do meio envolvente, o que facilita a sua adaptação em condições adversas. O desenvolvimento de biofilmes patogênicos em superfícies de contato nas áreas da saúde (próteses e instrumentos cirúrgicos) e alimentícia (equipamentos e tubulações) é a principal causa do acréscimo de contaminações que podem, eventualmente, originar problemas de saúde pública e de ordem econômica. Para prevenir, controlar ou erradicar biofilmes, alternativas tecnológicas v

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Cavenago, Bruno Cavalini. "O efeito do uso de fitoterápicos e da própolis nas propriedades físico-químicas, antimicrobiana e biocompatibilidade do MTA." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/25/25147/tde-22062015-090655/.

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O objetivo deste estudo foi avaliar as propriedades físico-químicas, antimicrobianas e biocompatibilidade do MTA branco manipulado com extratos aquoso e/ou em propilenoglicol da Arctium lappa L., Casearia sylvestris Sw. e própolis. Dentre os testes físico-químicos foram avaliados o tempo de presa, escoamento, pH, liberação de íons cálcio e alteração volumétrica. Para verificar o efeito antimicrobiano foram aplicadas as metodologias do contato direto (Enterococcus faecalis e a Cândida albicans) e da descontaminação dentinária, empregando a microscopia confocal de varredura laser para verificar

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Procópio, Andréa Lemos Falcão. "Efeito antimicrobiano residual e citotoxidade in vitro de resina acrílica para base de prótese após imersão prolongada em agentes de limpeza." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/25/25146/tde-26102015-103717/.

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O presente estudo in vitro objetivou avaliar a longo prazo o potencial antimicrobiano residual e a citotoxicidade de soluções químicas de limpeza de prótese quando incorporadas à resina acrílica termopolimerizável após sucessivos ciclos de imersão noturna diária. Discos (10mm x 1mm) de resina acrílica termopolimerizável para base de prótese (Lucitone 550) foram submetidos a três ciclos diários de desinfecção (8h/cada) em hipoclorito de sódio a 1% (NaClO), digluconato de clorexidina a 2% (CLX) ou água destilada (controle) durante 91 (T91) ou 183 dias (T183), simulando o período de 9 meses ou 1,

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Ramos, Wagner Soares. "Estudo de segurança e eficácia in vitro do ácido p-Cumárico e sua incorporação em emulsão cosmética óleo em água (O/A) /." Araraquara, 2019. http://hdl.handle.net/11449/181706.

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Orientador: Marcos Antonio Correa<br>Resumo: O ácido p-cumárico (APC) é um composto fenólico não flavonóide que apresenta segurança e eficácia bem descritas na ciência dos alimentos devido suas propriedades antioxidante, antitumoral, anti-inflamatória, antimicrobiana e por apresentar baixa toxicidade oral. Essas propriedades são pouco exploradas em produtos para cuidados com a pele. O objetivo do presente estudo foi avaliar de forma in vitro a segurança e a eficácia do APC e incorporá-lo em emulsão cosmética óleo em água (O/A). Nossos estudos in vitro e alternativos ao uso de animais foram con

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Lima, Marta Judite Nunes. "Avaliação da radiopacidade, do pH e da atividade antimicrobiana do MTA, do cimento Portland puro e do cimento Portland adicionado de agentes radiopacificadores." Universidade Federal de Sergipe, 2016. https://ri.ufs.br/handle/riufs/5904.

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Introduction: The radiopacity is a required property in all dental materials. This characteristic allows this materials be able to be distinguished from the tooth surfaces through the radiography. Portland cement (PC) presents physico-chemical and biological properties similar to mineral trioxide aggregate (MTA), except for radiopacifier. Objective: To evaluate the radiopacity, antimicrobial activity and pH of the MTA, pure PC and PC added different radiopacifiers. Materials and Methods: The radiopacity of MTA, pure PC and PC added the radiopacifying: iodoform, lead oxide, zirconium oxide, bis

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(7026050), Aline M. Elquist. "Instantaneous Antimicrobial Susceptibility Testing Using Piezoelectric Sensors." Thesis, 2019.

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Rapid determination of drugs effective against bacterial strains is critically important to stopping further spread of an infection and reducing antibiotic resistance. Antimicrobial susceptibility testing (AST) is done to determine what type of antibiotic and what concentration will be effective in treating an infection. Current, growth-dependent, AST methods are reliant on the growth rate of the bacteria and can take several days to several weeks to get results. A piezoelectric plate sensor can be used to measure an instant change in the minute physiological stresses of the bacteria cells whe

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"Rapid Antimicrobial Susceptibility Testing Based on Bacterial Motion Tracking." Doctoral diss., 2017. http://hdl.handle.net/2286/R.I.44980.

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abstract: Antibiotic resistant bacteria are a worldwide epidemic threatening human survival. Antimicrobial susceptibility tests (ASTs) are important for confirming susceptibility to empirical antibiotics and detecting resistance in bacterial isolates. Current ASTs are based on bacterial culturing, which take 2-14 days to complete depending on the microbial growth rate. Considering the high mortality and morbidity rates for most acute infections, such long time frames are clinically impractical and pose a huge risk to a patient's life. A faster AST will reduce morbidity and mortality rates, as

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Schoepp, Nathan Garrett. "Phenotypic Antimicrobial Susceptibility Testing Based on Nucleic Acid Analysis." Thesis, 2019. https://thesis.library.caltech.edu/11572/13/schoepp_nathan_2019_thesis.pdf.

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<p>Antimicrobial resistance (AMR) is one of the most widely recognized threats to global health, and one that continues to grow as new mechanisms of resistance evolve and resistant pathogens spread. Antibiotics are a cornerstone of modern medicine, but their misuse and overuse has constantly and consistently reduced their efficacy to the critically low levels we observe today. As a result, the rate of mortality as a direct result of AMR is approaching over a million deaths annually, with 20-year projections in the ten-millions. Rapid, phenotypic antimicrobial susceptibility testing (AST) that

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Hoe, Fernanda G. H. "Relationship between antimicrobial susceptibility testing and mastitis treatment outcomes." 2005. http://catalog.hathitrust.org/api/volumes/oclc/58539231.html.

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Chen, I.-Hsiung, and 陳意雄. "Identification and Antimicrobial Susceptibility Testing of Edwardsiella tarda Isolated from Anguilla japonica." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/62638462123098623210.

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碩士<br>國立嘉義大學<br>獸醫學系研究所<br>97<br>The identification and antimicrobial susceptibility testing of Edwardiella tarda were performed in this study. E. tarda were isolated from infected farms. Bacteria isolates were primarily screened on SS agar. Bacteria identification was then confirmed by the YM-NTUH computer coding system (which identifies glucose-fermenting Gram-negative bacilli) and commercially available API 20E kits. E. tarda genomic DNA can be detected by PCR in all of the 96 strains isolates. Analysis of their 16S rRNA sequences showed 100% similarity with E. tarda EIB 202 (ATCC15947) ref

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Kao, Wei-Lung, and 高維隆. "Identification and Antimicrobial Susceptibility Testing of Nocardia seriolae Isolated from Cultured Fish." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/53202617380618879160.

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碩士<br>國立嘉義大學<br>獸醫學系研究所<br>95<br>This study detect Nocardia seriolae by using polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP). Eighty isolates of N. seriolae from cultured fish was identified, together with a type strain N. seriolae BCRC 13745, were characterized by Sau-PCR. In addition, minimal inhibition concentrations (MICs) and inhibition zone diameter for ampicillin, amoxicillin, doxycycline, erythromycin, florfenicol, lincomycin and oxytetracycline were determined for each isolates based on Mueller–Hinton agar. Susceptibility data were tabulated in scatt

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CHOU, TYUI-YUN, and 周翠雲. "Antimicrobial susceptibility Testing and Strain Typing of Clinical Isolates of Mycobacterium avium." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/28395230437742034505.

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碩士<br>國立臺灣大學<br>醫事技術學研究所<br>88<br>Mycobacterium avium complex (MAC) group of microorganisms is the most common opportunistic bacterial pathogens causing disseminated disease in HIV infected patients. It is important to shorten the time to offer both identification and antimicrobial susceptibility testing on MAC, in that maybe much sustain to physicians which improve the therapy on disease. In this study, a total of 89 MAC isolates were recovered from 60 clinical patients, and were subject to rapid identification scheme by cellular fatty acid analysis by Gas Chromatography (GC), hsp65, Accuprob

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Costa, Eduardo Conceição. "Testing of antimicrobial activity of natural and chemical products against desulfovibrio piger." Master's thesis, 2014. http://hdl.handle.net/10451/38756.

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Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2014<br>Sulfate-reducing bacteria (SRB) are present in the intestinal flora of humans and animals and can induce several inflammatory diseases. Desulfovibrio piger is the main cause of Ulcerative Colitis for which there is no effective treatment. Due to inefficacy of antibiotics and to bacterial resistance there is a need for developing newer therapeutic alternatives that can invert this problem. It is known that plants do not develop as much infective diseases as humans or animals. Th

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Jhih-chengWang and 王致丞. "A Rapid Antimicrobial Susceptibility Testing based on Brownian Motion for Urinary Tract Infection." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/22pfdr.

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博士<br>國立成功大學<br>生物醫學工程學系<br>106<br>According to the 2013 survey of top ten leading causes of death reported by the Ministry of Health and Welfare, sepsis has become the 10th cause of women’s death in Taiwan. In the U.S., sepsis also ranks in the top ten leading causes of death. Except for the identification of pathogen (IP), the key for a successful treatment of sepsis is the antimicrobial susceptibility test (AST). Clinically, doctors are used to performing an empirical therapy before the AST comes clear. However, the blind uses of antibiotics usually enhance the drug resistance and, even wor

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Yu, Hsin-Chiao, and 游心喬. "Establishing Transgenic Tobacco Plants Expressing L-Amino Acid Oxidases of Trichoderma harziana and Testing Their Antimicrobial Effects." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/58677042500211272174.

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碩士<br>國立東華大學<br>生命科學系<br>101<br>The L-amino acid oxidases (LAAO) are flavoenzymes, which catalyze the stereospecific oxidative deamination of L-amino acid substrates. The LAAO have been demonstrated to exhibit antimicrobial activity. Dr. Peng purified a LAAO (ThLAAO) from Trichoderma harzianum ETS323. They showed that ThLAAO interact with bacteria to cause membrane permeabilization, increase exogenous H2O2 production, and cause bacterial damage. The purpose of this study is to genetically transform the ThLAAO gene into tobacco to elucidate whether ThLAAO alone can contribute antimicrobial effe

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Lin, Chao-Chi, and 林照琦. "Establishing Transgenic Tobacco Plants Expressing Biological Control Related Proteins of Trichoderma harziana and Testing Their Antimicrobial Effect." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/u2ys8x.

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碩士<br>國立東華大學<br>生命科學系<br>106<br>Trichoderma spp., a filamentous fungi, is commonly found in soil. It can inhibit the growth of various plant pathogenic fungi by inducing plant disease resistance. Therefore, it is widely used in biological control of plant diseases. Several lines of evidences have shown that the extracellular small protein 1 (SM1) and L-Amino acid oxidas (ThLAAO) secreted by Trichoderma spp. exhibit antimicrobial effects. The mechanism of disease resistance, however, is still uclear. This study is divided into two parts. First, we transform the ThSM1 gene into tobacco and explo

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Lowman, Warren. "Antimicrobial susceptibility testing and profiling of Nocardia species and other aerobic actinomycetes from South Africa: a comparative evaluation of broth microdilution versus the Etest." Thesis, 2015. http://hdl.handle.net/10539/17367.

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Gomes, Maria do Rosário Almeida. "Determinação da concentração mínima inibitória aos carbapenemos por citometria de fluxo." Master's thesis, 2018. http://hdl.handle.net/10400.14/31713.

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Entre os beta-lactâmicos, os carbapenemos são os antimicrobianos mais eficazes contra bactérias Gram-negativo. O aparecimento de bactérias multirresistentes ameaça seriamente essa classe de fármacos que salvam vidas. A determinação de concentrações mínimas inibitórias (CMI) aos antibióticos é atualmente considerada importante quer para o tratamento destas infeções quer para o controlo epidemiológico. Este estudo propõe um novo teste de suscetibilidade antimicrobiana baseado em citometria de fluxo para determinação rápida da CMI aos carbapenemos diretamente de hemoculturas positivas. Noventa e

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"Time-Lapse Large-Volume Light Scattering Imaging Cytometry." Doctoral diss., 2020. http://hdl.handle.net/2286/R.I.57113.

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abstract: Cytometry is a method used to measure and collect the physical and chemical characteristics of a population of cells. In modern medical settings, the trend of precision and personalized medicines has imposed a need for rapid point-of-care diagnostic technologies. A rapid cytometric method, which aims at detecting and analyzing cells in direct patient samples, is therefore desirable. This dissertation presents the development of light-scattering-based imaging methods for detecting and analyzing cells and applies the technology in four applications. The first application is tracking ph

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