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Journal articles on the topic 'Antioxidant - DPPH assay'

Author: Grafiati

Published: 7 June 2025

Last updated: 2 August 2025

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1

Gulcin, İlhami, and Saleh H. Alwasel. "DPPH Radical Scavenging Assay." Processes 11, no. 8 (2023): 2248. http://dx.doi.org/10.3390/pr11082248.

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Today, there is an increasing interest in antioxidants, especially to prevent the known harmful effects of free radicals in human metabolism and their deterioration during processing and storage of fatty foods. In both cases, natural-source antioxidants are preferred over synthetic antioxidants. So, there has been a parallel increase in the use of assays to estimate antioxidant efficacy in human metabolism and food systems. Today, there are many bioanalytical methods that measure the antioxidant effect. Of these, the 1,1-diphenyl-2-picrylhydrazil (DPPH) removing assay is the most putative, pop

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2

Sharma, Om P., and Tej K. Bhat. "DPPH antioxidant assay revisited." Food Chemistry 113, no. 4 (2009): 1202–5. http://dx.doi.org/10.1016/j.foodchem.2008.08.008.

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3

Jafri, Syed Anis Ali, Zafar Mahmood Khalid, Muhammad Zakryya Khan, and NaqeebUllah Jogezai. "Evaluation of phytochemical and antioxidant potential of various extracts from traditionally used medicinal plants of Pakistan." Open Chemistry 20, no. 1 (2022): 1337–56. http://dx.doi.org/10.1515/chem-2022-0242.

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Abstract The antioxidant potential of various extracts was evaluated using different antioxidant assays such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, ferric reducing antioxidant power (FRAP) assay, and 2,2-azinobis-ethylbenzothiozoline-6-sulphonic acid (ABTS) using UV spectrophotometer. The highest absorbance was observed in ethanolic extracts (EEs) of Euphrasia stricta 71.92 ± 1.22%, 65.77 ± 1.38%, and 67.88 ± 0.74%, followed by methanolic extracts (MEs) 70.14 ± 0.82%, 64.84 ± 0.74%, and 65.48 ± 1.40% for DPPH assay (517 nm), FRAP assay (700 nm), and ABTS assay (734 nm), respectively. T

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4

Yamauchi, Moeka, Yukino Kitamura, Haruka Nagano, Junya Kawatsu, and Hiroaki Gotoh. "DPPH Measurements and Structure—Activity Relationship Studies on the Antioxidant Capacity of Phenols." Antioxidants 13, no. 3 (2024): 309. http://dx.doi.org/10.3390/antiox13030309.

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The consumption of foods that are high in antioxidant capacity is believed to contribute to good health. Moreover, the addition of highly antioxidant compounds to foods is believed to prevent food deterioration. Among the known antioxidants in food, phenols have been identified as the primary antioxidants. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay is a simple, inexpensive, and rapid method widely used to evaluate the antioxidant capacity. Although the results of the DPPH assay depend on conditions such as the reaction time and concentration, the experimental conditions have not been stand

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Leki, Karol Giovani Battista, Yahya Febrianto, and Yithro Serang. "Evaluation of Antioxidant Activity in Solanum Ferox (Through DPPH and FRAP Assays)." JURNAL INFO KESEHATAN 23, no. 2 (2025): 309–17. https://doi.org/10.31965/infokes.vol23.iss2.1743.

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The antioxidant properties of natural compounds have garnered significant attention for their potential therapeutic applications. Solanum ferox, a plant traditionally used in several countries for medicinal purposes, has been identified as possessing potential antioxidant properties. However, the extent and efficacy of its antioxidant capacity have not been thoroughly examined, creating a gap in understanding its effectiveness compared to established antioxidants like ascorbic acid. This study aims to evaluate the antioxidant capacity of Solanum ferox using DPPH and FRAP assays across concentr

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6

Darshika, Acharya Meenakshi Vaidya*. "In-vitro antioxidant activity of hydroalcoholic extract of leaves of Hydnocarpus pentandrus (Buch. - Ham) Oken." International Journal of Pharmaceutical Sciences 2, no. 11 (2024): 167–72. https://doi.org/10.5281/zenodo.14030494.

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Cellular damage can arise from the reactions of free radicals with membrane lipids, nucleic acids, proteins, enzymes, and other micro molecules. Free radical-induced cell damage seems to be a primary factor in the aging process and degenerative diseases, including but not limited to cancer, heart disease, cataracts, liver disorders, diabetes mellitus, inflammation, and renal failure. Naturally, the body produces free radicals, and antioxidants scavenge them to shield the body from harmful consequences. This dynamic equilibrium exists between the two. It's possible that there aren't enough anti

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Hashim, Maha J., and Jeffrey R. Fry. "Evaluation of Direct and Indirect Antioxidant Properties of Selected Four Natural Chemical Compounds: Quercetin, Epigallocatechin-3-Gallate, Indole-3-Carbinol and Sulforaphane by DPPH Radical Scavenging Assay." Journal of Biomedical Research & Environmental Sciences 1, no. 8 (2020): 389–92. http://dx.doi.org/10.37871/jbres1170.

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The main characteristic of antioxidants is the capacity to scavenge free radicals produced during cell metabolism, and thus they prevent oxidative stress, which may reduce the risk of many diseases. In this study, we evaluate the antioxidant properties of selected four compounds Quercetin (Q), Epigallocatechin-3-Gallate, (EGCG), Indole-3-Carbinol (I3C) and Sulforaphane (SF) by DPPH assay. The view is to establish the distinction between direct and indirect antioxidants, which would be the form of the basis for subsequent cellular antioxidant assays in our further studies. For sample assay: 20

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8

Maha, J. Hashim* and Jeff rey R. Fry. "Evaluation of Direct and Indirect Antioxidant Properties of Selected Four Natural Chemical Compounds: Quercetin, Epigallocatechin-3-Gallate, Indole-3-Carbinol and Sulforaphane by DPPH Radical Scavenging Assay." Journal of Biomedical Research & Environmental Sciences 1, no. 8 (2020): 389–92. https://doi.org/10.37871/jbres1170.

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The main characteristic of antioxidants is the capacity to scavenge free radicals produced during cell metabolism, and thus they prevent oxidative stress, which may reduce the risk of many diseases. In this study, we evaluate the antioxidant properties of selected four compounds Quercetin (Q), Epigallocatechin-3-Gallate, (EGCG), Indole-3-Carbinol (I3C) and Sulforaphane (SF) by DPPH assay. The view is to establish the distinction between direct and indirect antioxidants, which would be the form of the basis for subsequent cellular antioxidant assays in our further studies. For sample assay: 20

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9

UROOJ, F., A. IFTIKHAR, K. MAZHAR, et al. "ANTIOXIDANT ASSESSMENT OF SOME THERAPEUTICALLY IMPORTANT PLANTS AS ANTI-AGING AGENTS." Biological and Clinical Sciences Research Journal 2024, no. 1 (2024): 969. http://dx.doi.org/10.54112/bcsrj.v2024i1.969.

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Skin, the protective outer covering of the body, acts as an insulator between internal and external environments. Skin diseases are a significant concern today, primarily due to oxidative stress. Although synthetic compounds and drugs are available, they often contain toxic and carcinogenic compounds, leading to cancer. Herbal and natural products, rich in natural antioxidants, are preferred as remedies. Objective: To evaluate the antioxidant potential of different solvent extracts at various concentrations from five plants: Aloe barbadensis, Camellia sinensis, Cocos nucifera, Ficus carica, an

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10

Ghosh, R., P. Barua, O. Sikder, S. Saha, S. Mojumder, and D. Sikdar. "Comparison of phenolic content and antioxidant activity of two common fruits of Bangladesh in solvents of varying polarities." Food Research 5, no. 6 (2021): 187–96. http://dx.doi.org/10.26656/fr.2017.5(6).253.

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Phenolic content and antioxidant activity of two common fruits of Bangladesh, namely Phyllunthus emblica and Elaeocarpus floribundus, were measured in water, methanol, ethanol, acetone and hexane extracts. Several in vitro models including phosphomolybdenum assay, DPPH free radical scavenging assay, FRAP assay and reducing power assay were used to assess the antioxidant activity of these extracts in comparison with reference antioxidants. Between the two fruits, P. emblica showed higher phenolic content and antioxidant activity in all the solvents used. In the DPPH scavenging assay, the activi

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Firuzi, Omidreza, Katayoun Javidnia, Maryam Gholami, Mohammad Soltani, and Ramin Miri. "Antioxidant Activity and Total Phenolic Content of 24 Lamiaceae Species Growing in Iran." Natural Product Communications 5, no. 2 (2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500219.

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The antioxidant activities of the methanolic extracts of 9 Salvia species and 15 other Lamiaceae plants growing in Iran were evaluated using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values ranged form 8.5 to 79.0 μM quercetin equivalents/g dry weight, and IC50 values in the DPPH assay from 115.7 to 1350.2 μg dry weight/mL. Salvia species showed the highest antioxidant activities. S. santolinifolia, S. eremophila and S. palestina, which have not been studied before, were the most active plants. These were more active

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12

Ariyarathna, Pathumi, Rizliya Visvanathan, Isuri Rathnayake, Terrence Madhujith, and Ruvini Liyanage. "In vitro antioxidant potential of eleven medicinal herbs in Sri Lanka: Correlation with phenols and flavonoids." International Journal of Secondary Metabolite 12, no. 3 (2025): 561–71. https://doi.org/10.21448/ijsm.1562753.

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Antioxidants play a crucial role in preventing and treating non-communicable diseases (NCDs) by scavenging free radicals. Medicinal herbs, used for centuries in traditional healthcare systems, have been gaining attention recently due to the negative effects of synthetic medicines. This study assessed the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of aqueous extracts from eleven commonly used Sri Lankan Ayurvedic plants and determined the relationship between their phenolic and flavonoid content with antioxidant activities. TPC and TFC were measured us

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Krishnan, Vijendren, Syahida Ahmad, and Maziah Mahmood. "Antioxidant Potential in Different Parts and Callus ofGynura procumbensand Different Parts ofGynura bicolor." BioMed Research International 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/147909.

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Plants from Gynura family was used in this study, namely,Gynura procumbensandGynura bicolor.Gynura procumbensis well known for its various medicinal properties such as antihyperglycaemic, antihyperlipidaemic, and antiulcerogenic; meanwhile,G. bicolorremains unexploited. Several nonenzymatic antioxidants methods were utilized to study the antioxidant capacity, which include ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, total flavonoid content, total phenolic content, and ascorbic acid content determination. DPPH assay revealsG. procumbe

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14

Dewaantari, Virdah Dwi, Setyabudi Setyabudi, and Kun Ismiyatin. "Antioxidant Potential of Epigallocatechin-3-gallate, Ascorbic Acid, and Sodium Ascorbate in Solution and Gel Forms by 2,2-diphenyl-1-picrylhydrazyl (DPPH) Assay." Conservative Dentistry Journal 11, no. 1 (2021): 19. http://dx.doi.org/10.20473/cdj.v11i1.2021.19-23.

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Background: Free radicals are molecules without any electron pairs, unstable, and highly reactive. Antioxidants are needed to reduce free radicals. Antioxidants provide various benefits in dentistry as a preventive agent for caries, healing, bone formation, mouthwash, preventive and therapeutic cancer, and reduction of periodontal disease progressions. Additionally, research on antioxidants is still undergone due to the existence of free radical residues on bleached teeth. Epigallocatechin-3-gallate (EGCG), ascorbic acid (AA), and sodium ascorbate (SA) are ingridients that have antioxidant pro

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15

Luo, Aoxue, Ping Peng, Wen Feii, Linying Yang, and Yijun Fan. "Isolation and antioxidant activity (in vitro and in vivo) of the flavonoid from Tartarian-buckwheat." Journal of Scientific and Innovative Research 3, no. 2 (2014): 168–72. http://dx.doi.org/10.31254/jsir.2014.3210.

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The total flavonoid was isolated from tartarian-buckwheat through reflux extraction. In order to explore the antioxidant activities, in the present study, our study aimed to examine the antioxidant activity of the total flavonoid using different assays including: reducing power, 2,2- diphenyl 1-picrylhydrazyl (DPPH) radical scavenging assay, ABTS radical scavenging assay and hydroxyl radical scavenging activity. The results exhibited that the total flavonoid has significant reductive ability and radicals scavenging effects. In vivo antioxidant activities assays, the flavonoid was found to incr

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16

Lanez, Touhami, and Abdelkerim Rebiai. "Development of an Electrochemical Method for the Measurement of Antioxidant Capacity of Pure Compounds and Natural Substances Extracts." International Letters of Chemistry, Physics and Astronomy 6 (September 2013): 46–54. http://dx.doi.org/10.18052/www.scipress.com/ilcpa.6.46.

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A new method has been developed to measure total antioxidant activity of antioxidants in foods and natural substances without use of standard antioxidants and without use of calibration curves plotting. It is based on measuring the oxidation peak current of superoxide anion radical electrochemically generated by reduction of commercial molecular oxygen in dimethylformamide. The method has been validated using 7 known standard antioxidants and the results have been compared with those obtained by the DPPH and molybdate ion reduction assays. Measured antioxidant capacities were highly correlated

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Lanez, Touhami, and Abdelkerim Rebiai. "Development of an Electrochemical Method for the Measurement of Antioxidant Capacity of Pure Compounds and Natural Substances Extracts." International Letters of Chemistry, Physics and Astronomy 6 (January 10, 2013): 46–54. http://dx.doi.org/10.56431/p-8gy7y0.

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A new method has been developed to measure total antioxidant activity of antioxidants in foods and natural substances without use of standard antioxidants and without use of calibration curves plotting. It is based on measuring the oxidation peak current of superoxide anion radical electrochemically generated by reduction of commercial molecular oxygen in dimethylformamide. The method has been validated using 7 known standard antioxidants and the results have been compared with those obtained by the DPPH and molybdate ion reduction assays. Measured antioxidant capacities were highly correlated

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18

HDT, Madhuranga. "Advancing In vitro Antioxidant Activity Assessment: A Comprehensive Methodological Review and Improved Approaches for DPPH, FRAP and H2O2 Assays." Journal of Natural & Ayurvedic Medicine 7, no. 4 (2023): 1–7. http://dx.doi.org/10.23880/jonam-16000431.

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The study of antioxidants and their implications in numerous sectors, from food engineering to medicine and pharmacy, is of great interest to the scientific community. The presence of free radicals in the body has been linked to various human diseases. Fortunately, antioxidants can counteract these harmful molecules and reduce their negative impact. This highlights the importance of researching natural antioxidants found in plants. Free radicals are closely tied to the development of conditions like diabetes, cancer, and heart and neurological disorders. Exploring plants could lead to the disc

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19

Tuty S, Fidrianny I, and Sukrasno. "Antioxidant profile of chicken bile extract and correlation with chemical content." International Journal of Research in Pharmaceutical Sciences 10, no. 3 (2019): 2311–17. http://dx.doi.org/10.26452/ijrps.v10i3.1470.

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Component in chicken bile may be similar to bear bile, which contains an immunostimulant agent ursodeoxycholic acid and related to antioxidant effect. The purposes of this study were to evaluate the antioxidant potential of chicken bile extract by the DPPH method, analyze the correlation between total phenolic and flavonoid content with IC50 of DPPH scavenging activities. Chicken bile was cut and dried using freeze dryer, then extracted by reflux using different polarity solvents. A rotary evaporator was used to concentrate the extracts. Calculation of total flavonoid and phenolic content, ant

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20

Widowati, Wahyu, Teresa Liliana Wargasetia, Teddy Markus Zakaria, et al. "Antioxidant Activity of TEMON (Clitoria ternatea and Citrus sp.) as an Infused Herbal Tea." Majalah Obat Tradisional 27, no. 1 (2022): 32. http://dx.doi.org/10.22146/mot.71628.

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Many people around the world believed that herbal tea had a lot of antioxidants contained in it, thus it was widely drunk all over the world. However, the antioxidant capacities of herbal tea may be various because it is affected by the usage of different plants as its primary ingredient. As a result, this study examines the antioxidant activity of herbal tea made from dried Citrus sp. and Clitoria ternatea flower petals. The antioxidant potential was assessed by performing a DPPH and H2O2 scavenging activity assay on 2,2-diphenyl-1picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging

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21

Platzer, Melanie, Sandra Kiese, Thomas Herfellner, Ute Schweiggert-Weisz, Oliver Miesbauer, and Peter Eisner. "Common Trends and Differences in Antioxidant Activity Analysis of Phenolic Substances Using Single Electron Transfer Based Assays." Molecules 26, no. 5 (2021): 1244. http://dx.doi.org/10.3390/molecules26051244.

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Numerous assays were developed to measure the antioxidant activity, but each has limitations and the results obtained by different methods are not always comparable. Popular examples are the DPPH and ABTS assay. Our aim was to study similarities and differences of these two assay regarding the measured antioxidant potentials of 24 phenolic compounds using the same measurement and evaluation methods. This should allow conclusions to be drawn as to whether one of the assays is more suitable for measuring specific subgroups like phenolic acids, flavonols, flavanones, dihydrochalcones or flavanols

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22

Shivanki, Aggarwal*1 &. Sheel Sharma2. "EVALUATION OF ANTIOXIDANT ATTRIBUTES OF ORANGE (CITRUS SINENSISL.)." INTERNATIONAL JOURNAL OF ENGINEERING SCIENCES & RESEARCH TECHNOLOGY 6, no. 6 (2017): 607–12. https://doi.org/10.5281/zenodo.817974.

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Phytochemicals are gaining unprecedented recognition as cardinal food constituentsto effectively mitigate rampant scenario of chronic, degenerative diseases. This study attempted to explore the antioxidant attributes of Orange (Citrus sinensisL.) in three categories: nutrient antioxidants estimation i.e. vitamin C and β-carotene, phytochemical quantification i.e. total phenols and flavonoids, and in vitro antioxidant assays expressed in terms of DPPH (2,2-Diphenyl-1-picrylhydracyl) radical scavenging assay, ABTS (2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging assay

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23

Angeli, Lucrezia, Sebastian Imperiale, Yubin Ding, Matteo Scampicchio, and Ksenia Morozova. "A Novel Stoichio-Kinetic Model for the DPPH• Assay: The Importance of the Side Reaction and Application to Complex Mixtures." Antioxidants 10, no. 7 (2021): 1019. http://dx.doi.org/10.3390/antiox10071019.

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The 2,2-diphenyl-1-picrylhydrazyl (DPPH•) assay is widely used to determine the antioxidant activity of food products and extracts. However, the common DPPH• protocol uses a two-point measurement and does not give information about the kinetics of the reaction. A novel stoichio-kinetic model applied in this study monitors the consumption of DPPH• by common antioxidants following the second order reaction. The fitting of such decay yields the rate constant k1, which describes the main reaction between antioxidants and DPPH•, and the rate constant k2, which is attributed to a slower side reactio

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24

Zhang, Xiu-Li, Yong-Dong Zhang, Tao Wang, Hong-Yun Guo, Qi-Ming Liu, and Hai-Xiang Su. "Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical Methods." Journal of Chemistry 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/249485.

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Several assays have been frequently used to estimate antioxidant capacities includingABTS•+, DPPH, and FRAP assays. Xanthohumol (XN), the major prenylated flavonoid contained in beer, witnessed various reports on its antioxidant capacity. We systematically evaluated the antioxidant activity of XN using three systems, 2,2,-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS•+) scavenging assays, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assays, and ferric reducing antioxidant power (FRAP) assays. The results are expressed as Trolox equivalent antioxidant capacity (TEAC). The TEAC of XN wa

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25

Ali Hassan, Siti Hawa, and Mohd Fadzelly Abu Bakar. "Antioxidative and Anticholinesterase Activity ofCyphomandra betaceaFruit." Scientific World Journal 2013 (2013): 1–7. http://dx.doi.org/10.1155/2013/278071.

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Cyphomandra betaceais one of the underutilized fruits which can be found in tropical and subtropical countries. This study was conducted to determine the antioxidant activity and phytochemical contents in different parts (i.e., flesh and peel) of the fruits. Antioxidants were analyzed using DPPH and ABTS free radical scavenging assays as well as FRAP assay. Anticholinesterase activity was determined using enzymatic assay using acetyl cholinesterase enzyme. For 80% methanol extract, the peel of the fruit displayed higher antioxidant activity in both FRAP and ABTS free radical scavenging assays

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26

Ali Ahmad and Abdullahi Muhammad Abdu. "Evaluation of Toxicity and Antioxidant Activity of Hydro-Ethanol Leave Extract of Mentha piperita." Sahel Journal of Life Sciences FUDMA 1, no. 1 (2023): 205–11. http://dx.doi.org/10.33003/sajols-2023-0101-022.

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Mentha piperita has numerous pharmacological, cosmetic and alimental applications due to its ability to produce terpene and terpenoid compounds. Antioxidants are considered as important bioactive compound on account of many health benefits along with their vital role in delaying oxidative rancidity of numerous food. The study was to investigate acute toxicity evaluation and in vitro antioxidant activity of hydro-ethanol leave extracts of M. piperita. The acute toxicity was evaluate in rat according to (OECD). Animals were daily observed for 24 hours after treatment for mortality. The antioxida

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27

Devi, Khoirom Ratipiyari, Paonam Priyobrata Singh, Moirangthem Medhapati Devi, and Gurumayum Jitendra Sharma. "Evaluation of Antioxidant Activities of Alpinia galanga (L.) Willd." Biosciences Biotechnology Research Asia 15, no. 4 (2018): 899–908. http://dx.doi.org/10.13005/bbra/2700.

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Present research was designed to evaluate the free radical scavenging capacities and antioxidant activities of rhizome extracts of Alpinia galanga prepared in different solvent systems (60% aqueous methanol, 60% aqueous ethanol and distilled water) using different in vitro chemical assays. Antioxidant components such as total phenolic content (TPC), total flavonoid content (TFC) and ascorbic acid contents of the ginger species were screened. Antioxidant assays employed included sulphur free radical reactivity assay, ferric ion reducing power assay, DPPH free radical scavenging capacity assay,

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Ren, Jian, and Zhen Yu Wang. "In Vitro Antioxidant Activity of Pigment Extracted from Fruits of Padus racemosa and Padus virginiana." Advanced Materials Research 183-185 (January 2011): 565–69. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.565.

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The total anthocyanin content (TAC) of pigment extracted from fruits of Padus racemosa and Padus virginiana was determined by pH-differential method. TAC in pigment from fruits of Padus racemosa was higher than that in Padus virginiana. The vitro antioxidant activity of the two kinds of pigment was evaluated by different assays, including DPPH• assay, ABTS•+assay, OH• assay and reducing power assay. The results showed that except for reducing power, pigment from fruit of Padus racemosa showed stronger ABTS•+, DPPH• and OH• radical scavenging activity than pigment from fruit of Padus virginiana

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Shekade, Pratiksha Prabhakar, Sumedh Sunil Chavan, and Arvind Shankarrao Dhabe. "Antioxidant Potential of Arogyavardhini Vati Extract by DPPH Assay." Journal of Drug Delivery and Therapeutics 15, no. 7 (2025): 25–28. https://doi.org/10.22270/jddt.v15i7.7254.

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Arogyavardhini Vati, an Ayurvedic medicine, was commonly used during the COVID-19 pandemic for its immunomodulatory and therapeutic properties. The DPPH radical scavenging assay in this study revealed its antioxidant potential. This Ayurvedic medicine demonstrated considerable antioxidant capability with 78.04% DPPH scavenging activity. These findings support its traditional use and suggest the need for further investigation. Keywords: Arogyavardhini Vati, DPPH assay, antioxidant activity, ethanol extract, COVID-19, Soxhlet’s extraction, etc.

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Farias, K. S., T. S. N. Santos, M. R. A. B. Paiva, et al. "Antioxidant properties of species from the Brazilian cerrado by different assays." Revista Brasileira de Plantas Medicinais 15, no. 4 (2013): 520–28. http://dx.doi.org/10.1590/s1516-05722013000400008.

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The purpose of this study was to screen the antioxidant activity of medicinal plant extracts from the Brazilian cerrado, through other methods than the total phenolic content and its correlation with the antioxidant activity. Ethanolic extracts of ten species were evaluated through three antioxidant assays, in vitro, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), total antioxidant activity and reducing power; and by using the Folin-Ciocalteu method the total phenolic content was determined. Ethanolic extracts of Stryphnodendron obovatum, Cecropia pachystachya and Duguetia furfuraceae showed s

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Pugalenthi, M., M. Pradheeba, S. Vishnu Kumar, G. Vasukipriyadharshini, S. Swathi, and G. Divya Bharathi. "UNVEILING THE PHYTOCHEMICAL PROFILE, SECONDARY METABOLITE QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF CLEMATIS WIGHTIANA WALL. EX WIGHT and ARN." Journal of Advanced Scientific Research 13, no. 03 (2022): 70–77. http://dx.doi.org/10.55218/jasr.202213312.

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Traditionally, the leaves of Clematis wightiana have been used in the treatment of rheumatism, indigestion, headaches, varicose veins, bone problems, nasal congestion and sinus. The present study was conducted to evaluate the phytochemical profile, quantification of secondary metabolites and free radical scavenging capacity of C. wightiana leaf. The total phenolic, tannin and flavanoid content of C. wightiana leaves were quantified and were found to be higher in the ethyl acetate extract. Subsequently, the extracts were subjected to appraise their antioxidant capacity by availing various in vi

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32

Ishola, Oluwatoyin, IGE EMMANUEL OJO, Ezekiel Tosin Babatunde, Iyiola, Aanuoluwa Temitayo, Taiwo Oluwole FABIYI, and Abdulgafar Ahmad Adeyanju. "Determination of Antioxidant Capacity in Aqueous Extracts of Corymbia citriodora Using DPPH, ABTS, FRAP, TPC, and Hydrogen Peroxide Assays." Asian Journal of Research in Biochemistry 15, no. 3 (2025): 171–78. https://doi.org/10.9734/ajrb/2025/v15i3398.

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The increasing interest in natural antioxidants has led to extensive research on plant extracts with potential health benefits. This study evaluates the antioxidant capacity of aqueous extracts of Corymbia citriodora using five different assays: DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay, FRAP (Ferric Reducing Antioxidant Power), Total Phenolic Content (TPC) via the Folin-Ciocalteu method, and Hydrogen Peroxide Scavenging assay. The results indicate that the extract possesses significant a

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Sukrasno, Sukrasno, Slamet Tuty, and Irda Fidrianny. "ANTIOXIDANT EVALUATION AND PHYTOCHEMICAL CONTENT OF VARIOUS RICE BRAN EXTRACTS OF THREE VARIETIES RICE FROM SEMARANG-CENTRAL JAVA, INDONESIA." Asian Journal of Pharmaceutical and Clinical Research 10, no. 6 (2017): 377. http://dx.doi.org/10.22159/ajpcr.2017.v10i6.16565.

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Objectives: The objectives of this research were to evaluate antioxidant activity from different polarities rice bran extract of three varieties of rice using two methods of antioxidant testing which were FRAP (Ferric Reducing Antioxidant Power) and DPPH (2,2-diphenyl-1-picrylhydrazyl), and correlation of total phenolic, flavonoid and carotenoid content with their EC50 of FRAP and IC50 of DPPH antioxidant activities. Methods: Extraction was conducted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Determination of total phenolic, flavonoid and

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Xie, Yulu, Xican Li, Jingyu Chen, Yuman Deng, Wenbiao Lu, and Dongfeng Chen. "pH Effect and Chemical Mechanisms of Antioxidant Higenamine." Molecules 23, no. 9 (2018): 2176. http://dx.doi.org/10.3390/molecules23092176.

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In this article, we determine the pH effect and chemical mechanism of antioxidant higenamine by using four spectrophotometric assays: (1) 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide radical (PTIO•)-scavenging assay (at pH 4.5, 6.0, and 7.4); (2) Fe3+-reducing power assay; (3) Cu2+-reducing power assay; and (4) 1,1-diphenyl-2-picryl-hydrazyl (DPPH•)-scavenging assay. The DPPH•-scavenging reaction product is further analyzed by ultra-performance liquid chromatography, coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) technol

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P, SHISA ULLAS, NAMITA NAMITA, KANWAR P. SINGH, et al. "Influence of drying techniques on retention of anthocyanin and their antioxidant activities in chrysanthemum (Chrysanthemum x morifolium) flowers." Indian Journal of Agricultural Sciences 88, no. 2 (2018): 228–33. http://dx.doi.org/10.56093/ijas.v88i2.79189.

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The influence of different drying methods, viz. shade drying, hot air oven drying and microwave oven drying on total anthocyanin content, total phenolic content and their antioxidant activities (measured by CUPRAC, FRAP and DPPH assay) of dried ray florets of five anthocyanin rich chrysanthemum (Chrysanthemum × morifolium Ramat.) varieties was studied in the present investigation. Microwave oven drying was the best method w.r.t. retaining its total anthocyanin content, total phenolic content and antioxidant activities. Microwave oven drying method had retained highest total anthocyanins (208.2

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Tahir, Muhammad, Muhammad Shahab Khan, Muhammad Bilal, Ashfaq Ahmad, and Mukhtar Ahmad. "In-Vitro Antioxidant Activites of Novel Synthesized Copper (II) Complexes." International Journal of Current Research and Review 14, no. 13 (2022): 60–64. http://dx.doi.org/10.31782/ijcrr.2022.141310.

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Introduction: The term radical is originally implied, to a portion of a molecule which cannot exist independently in nature. The Development of these radicals is driven by thermal hemolysis, high energy radiation and photolysis. Free radical attacks cause cell damage and homeostatic disruption. Targets of free radicals are all kinds of molecules in the body, such as lipids, nucleic acids and proteins are the major targets. Antioxidants are substances capable of slowing or stopping oxidation pathways that occur under the effect of reactive oxygen moieties or free atmospheric oxygen. Objectives:

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Geng, Rui, Lei Ma, Liangliang Liu, and Yixi Xie. "Influence of Bovine Serum Albumin-Flavonoid Interaction on the Antioxidant Activity of Dietary Flavonoids: New Evidence from Electrochemical Quantification." Molecules 24, no. 1 (2018): 70. http://dx.doi.org/10.3390/molecules24010070.

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Interaction between dietary flavonoids and albumins plays an important role in the bioavailability and bioactivity of flavonoids. Therefore, the influence of this interaction on the antioxidant activity of flavonoid has attracted much interest. In this study, a ceric reducing/antioxidant capacity assay (CRAC) was employed to investigate the effects of albumin-flavonoid interaction on the antioxidant activity of seven common flavonoids. The results obtained from the CRAC assay were also compared separately with the results from the spectrophotometric methods including 2,2-diphenyl-1-picrylhydra

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Giamperi, Laura, Anahi Bucchini, Angela Bisio, Emanuela Giacomelli, Giovanni Romussi, and Donata Ricci. "Total Phenolic Content and Antioxidant Activity of Salvia spp. Exudates." Natural Product Communications 7, no. 2 (2012): 1934578X1200700. http://dx.doi.org/10.1177/1934578x1200700221.

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The total phenolic content and antioxidant activity of 6 Salvia spp. exudates were measured to find new potential sources of natural antioxidants. Total phenolic content was assessed by a modified Prussian blue method, and the antioxidant activity by two methods: 1,1- diphenyl-2-picryl- hydrazyl (DPPH) radical scavenging capacity assay and lipoxygenase inhibitory assay. The total phenolic content ranged between 1.3 μg/mg DW ( S. fallax) and 74.0 μg/mg DW ( S. cacaliaefolia). In the DPPH test, S. cacaliaefolia was more effective than BHT, while in the inhibition of lipid peroxidation all the ex

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Al-Mansori, Bubaker, Wessal Hassan El-Ageeli, Seham Hamad Alsagheer, and Faheem A. F. Ben-Khayal. "Antioxidant Activity- Synergistic Effects of Thymol and Carvacrol." Al-Mukhtar Journal of Sciences 35, no. 3 (2020): 185–94. http://dx.doi.org/10.54172/mjsc.v35i3.271.

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This study was conducted to evaluate the antioxidant activity-synergistic effects of Thymol and Carvacrol using Three assays DPPH, FRAP, and TEAC. In the DPPH assay Carvacrol, Thymol and Carvacrol-Thymol mixture had scavenging activity and this activity increasing by increasing concertation from 50 to 1000ppm. Carvacrol showed a strong antioxidant activity than Thymol, and no synergistic effect in their mixture at tested concertation. In FRAP assay, Carvacrol had the highest antioxidant activity as in DPPH assay result. However, the mixture of Carvacrol and Thymol showed a higher reducing powe

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Laaroussi, Hamid, Abdelouahad Aouniti, Ouafae Mokhtari, et al. "Experimental and Theoretical Investigations of Argania spinosa’s Extracts on the Antioxidant Activity and Mild Steel Corrosion’s Inhibition in 1 M HCl." Applied Sciences 12, no. 24 (2022): 12641. http://dx.doi.org/10.3390/app122412641.

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The aim of the present research is the evaluation of the extraction process effect on the chemical composition, the antioxidant activities, and the mild steel corrosion inhibition ability of Argania spinosa’s extracts (alimentary oil (AO) and hexanic extract of roasted almonds (HERA)). The chemical composition revealed that both extracts have the same major compounds: Palmitic, linoleic, and stearic acids, with their order slightly different. Electrochemical impedance spectroscopy (EIS), weight loss measurements, and polarization curves were used to estimate AO and HERA’s mild steel corrosion’

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Ugwo, Jeremiah, and Marvellous Kehinde Adealawode. "Determination of Antioxidant activities of locally produce rice in Northern and Western part of Nigeria, using Kebbi rice and Igbemo rice as case study." Catalyx : Journal of Process Chemistry and Technology 1, no. 1 (2024): 33–44. http://dx.doi.org/10.61978/catalyx.v1i1.238.

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This research provides the antioxidant activities of locally produced rice in the Northern and Western regions of Nigeria, with a focus on Kebbi rice and Igbemo rice. The study utilizes the Ferric Reducing Antioxidant Power (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assays to evaluate the antioxidant capacity of these rice varieties. In which the sample was extracted using solvent extraction method after which the antioxidant (AO) activity of the rice samples as ferric-reducing power, is determined using a modified FRAP (ferric reducing/antioxidant power) assay Sodium phosphate, buffer so

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Turiman, N. S. R., S. A. A. Muttalib, W. B. Sunarharum, Raseetha S., and E. T. T. Tan. "Antioxidant potencies in enzymatic hydrolysates from cup and corner sections of edible bird's nest." Food Research 8, Supplementary 8 (2025): 14–21. https://doi.org/10.26656/fr.2017.8(s8).10.

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Edible Bird's Nest (EBN) has many health-promoting properties, including antioxidants. Although the antioxidant properties of the EBN hydrolysates have been reported in the previous literature, little study has been done on the antioxidant potencies of the enzymatic hydrolysates within different sections of EBN. Thus, this study investigated the antioxidant potencies of the enzymatic hydrolysates from both the cup and corner sections of EBN. Therefore, using different enzymatic hydrolysis durations (1, 2, and 3 hr), this study examined the antioxidant potencies of enzymatic hydrolysates from c

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Ajaib, Muhammad, Tanveer Hussain, Saleha Farooq, and Mehrban Ashiq. "Analysis of Antimicrobial and Antioxidant Activities of Chenopodium ambrosioides: An Ethnomedicinal Plant." Journal of Chemistry 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/4827157.

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The antimicrobial and antioxidant potential of Chenopodium ambrosioides L. was explored. Antimicrobial potential was investigated through zone of inhibition and minimum inhibition concentration whereas antioxidant potential of selected plant was evaluated through different techniques, that is, total phenolic contents, total flavonoids content, DPPH assay, ABTS assay, and metal chelating. It is concluded that both parts showed good to satisfactory antimicrobial and antioxidant results. The maximum antibacterial potential is exhibited by bark macerated in petroleum ether against Bacillus subtili

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Скрыпник (Skrypnik), Любовь (Lyubov') Николаевна (Nikolaevna), and Алина (Alina) Андреевна (Andreevna) Курашова (Kurashova). "THE COMPARATIVE STUDY OF ANTIOXIDANT PROPERTIES OF SOME SAMBUCUS L. SPECIES." chemistry of plant raw material, no. 1 (March 6, 2019): 127–37. http://dx.doi.org/10.14258/jcprm.2019014037.

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The antioxidant properties of fruits, flowers, leaves, bark (or stem) of elderberry (Sambucus nigra L.), red elderberry (Sambucus racemosa L.) and dwarf elder (Sambucus ebulus L.) were investigated. The total content of anthocyanins and total phenolic compounds content by using of Folin-Ciocalteu assay were determined spectrophotometrically. The total content of water-soluble antioxidants was investigated by amperometric method. The antioxidant activity (AOA) of plant extracts was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2’azinobis(3)ethylbenzthiazoline-6- sulfonic

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Nazri, Miza Badriah, Azrina Azlan, Sharmin Sultana, and Rozita Yahya. "Correlation of Antioxidant Properties Between Immature and Mature Okra (Abelmoschus Esculentus) Fruits." Indonesian Journal of Agricultural Research 7, no. 1 (2024): 68–78. http://dx.doi.org/10.32734/injar.v7i1.13351.

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This study aimed to assess and compare the antioxidant activity and content (total flavonoid levels and total phenolic) of mature and immature okra. The antioxidant activity of okra fruits was assayed using four methods, namely: Aluminium Chloride Colorimetric assay, Folin-Ciocalteu assay, 1, 1-diphenyl-2-picrylhydrazyl (DPPH), and Reducing Antioxidant Power assay (FRAP) assays. The immature, mature, and very mature okra samples (less than 8 days, 10-15 days, and more than 20 days, respectively) were extracted using two different solvents (65% ethanol and water). The sample that was extracted

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*Naila, Ali *Humera Afrasiab *Farkhanda Jabeen. "ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACTS OF INDIGENOUS AND EUROPEAN GRAPE VARIETIES FROM PAKISTAN." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 09 (2018): 9431–39. https://doi.org/10.5281/zenodo.1443424.

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<em>Grapes are rich in phenolic compounds, both flavonoids and non-flavonoids. The aim of the present study is to evaluate antioxidant of methanol extract of Grape leaves of different indigineous and European varieties of Pakistan. The results showed that grape leaves extract had clear antioxidants activities. </em><em>The antioxidant activities of all the extracts were examined usung three complementary methods, namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)scavenging assay and reducing power scavenging assay. Although all the varieties

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Opena, Edward Laurenc, Matilda Cempron, Johndel Remon, Reggie Dela Cruz, and Junge Guillena. "Quantitation of antioxidant levels of soy-fern fermentation by DPPH assay." Palawan Scientist 17, no. 1 (2024): 26–34. http://dx.doi.org/10.69721/tps.j.2025.17.1.04.

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Fermentation biotechnology is one of the approaches to addressing the issues of food security worldwide, where the demand for healthier and safer foods is becoming mainstream. Even though fermentation has been practiced since ancient times, there are still an infinite number of topics that can serve as subjects for fermentative investigation. Among the popular nutraceutical research is the antioxidant properties of plants. In this study, the radical scavenging activities of soy-fern fermentation by mixed probiotics (13 species) have been quantified using the 2,2-diphenyl-1-picrylhydrazyl (DPPH

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Kumar A, Ajesh, S. S. Syed Abuthahir, and Hassan Y. Aboul-Enein. "Phytochemical extraction and comparative analysis of antioxidant activities of Areca catechu L. nut extracts." Pharmacia 69, no. 2 (2022): 447–51. http://dx.doi.org/10.3897/pharmacia.69.e77829.

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FRAP assay proved all the extracts of Areca catechu L. nut have antioxidant properties because IC50 values of all the extracts of the same were less than that of ascorbic acid. Remaining antioxidant assays like DPPH radical scavenging assay, H2O2 scavenging assay, and Fe2+ chelating assay showed more antioxidant properties in ethyl acetate extract and nonpolar solvent extracts like n- hexane, and chloroform respectively. Antioxidant properties of Areca catechu L. nut varied depending upon the different solvent extract.

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Suhendy, Hendy, Muhamad Insanu, and Irda Fidrianny. "Extracts, Fractions, and Subfractions from Purple-Orange Sweet Potato (Ipomoea batatas L.): Xanthine Oxidase Inhibitory Potential and Antioxidant Properties." Molecules 30, no. 11 (2025): 2442. https://doi.org/10.3390/molecules30112442.

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Previous research has shown that fractions outperformed extracts in pharmacological activity and safety. This study assessed the total phenol and flavonoid content, as well as antioxidant and xanthine oxidase inhibitory (XOI) activities, of purple-orange sweet potato extracts, fractions, and subfractions. Using UV-visible spectrophotometry, the leaves showed the highest values for total phenol, flavonoid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), Cupric Ion Reducing Antioxidant Capacity (CUPRAC) assays, and XOI activity. The sequential extraction of the le

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Singh, Charanjeet, Dharmendra Ahuja, and SC Mehta. "Assessment of the In-vitro antioxidant activity of the alcoholic extract/fractions of Bombax malabaricum DC." Journal of Drug Delivery and Therapeutics 9, no. 4 (2019): 135–40. http://dx.doi.org/10.22270/jddt.v9i4.2989.

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Various parts of Bombax malabaricum are used in traditional Indian medicine for the treatment of inflammatory diseases, diarrhea, fever, acute swelling and diuretic. Current research on Bombax malabaricum DC is aimed at finding of natural plants derived from antioxidants that provide sustenance through additional components or synergistic training as the plant's cellular reinforcements are necessary to prevent the movement of interfaced free radical problems. The antioxidant activity in Bombax malabaricum DC can be detected by alcoholic extract fraction using unique laboratory models. These in

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