Academic literature on the topic 'Antiradical activity'
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Journal articles on the topic "Antiradical activity"
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Karamać, M., A. Buciński, B. Pegg R, and R. Amarowicz. "Antioxidant and antiradical activity of ferulates." Czech Journal of Food Sciences 23, No. 2 (November 15, 2011): 64–68. http://dx.doi.org/10.17221/3373-cjfs.
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Antioxidant and antiradical activities of ferulates (i.e., ferulic acid, isoferulic acid, coniferyl aldehyde, and methyl ferulate) were investigated using a β-carotene-linoleate model system and a DPPH radical scavenging assay, respectively. Compounds so tested exhibited antioxidant and antiradical properties to varying degrees. Methyl ferulate showed the strongest antioxidant activity, whereas the parent phenolic acid was the most active ferulate to scavenge the DPPH radical (DPPH<sup>·</sup>). Isoferulic acid at concentrations ranging from 10 to 100 nmol/assay did not impart an antiradical efficacy; this may be attributed to the location of the hydroxyl group in the meta position on the aromatic ring.
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Daglia, M., A. Papetti, and G. Gazzani. "Green and roasted coffee antiradical activity stability in chemical systems." Czech Journal of Food Sciences 22, SI - Chem. Reactions in Foods V (January 1, 2004): S191—S194. http://dx.doi.org/10.17221/10658-cjfs.
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The stability to storage at different temperature and oxygen exposure of green and roasted coffee either as coffee beans or as ground coffee antiradical activity, was evaluated. The results showed that the coffee solution antihydroxyl radical activity was constant, independently from the coffee species, from the roasting process, and moreover from the type of storage conditions, suggesting that temperature and oxygen exposure did not affect this antiradical activity. With regard to antiperoxyl radical activity, all green coffee solutions showed remarkable and stable activity. Conversely, the roasted coffee beans and roasted and ground coffee antiperoxyl radical activity started to increase after three month of storage, suggesting that Maillard reaction products affect the stability of such antiradical property.
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Vol’eva, V. B., I. S. Belostotskaya, N. L. Komissarova, A. V. Malkova, T. V. Pokholok, and E. Ya Davydov. "Antiradical activity of dioxolane derivatives." Russian Journal of Organic Chemistry 49, no. 3 (March 2013): 446–49. http://dx.doi.org/10.1134/s1070428013030226.
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Vladimirov, Yu A., �. A. Parfenov, O. M. Epanchintseva, and L. D. Smirnov. "Antiradical activity of coumarin ruductones." Bulletin of Experimental Biology and Medicine 112, no. 5 (November 1991): 1560–63. http://dx.doi.org/10.1007/bf00840412.
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Lachman, J., M. Šulc, J. Sus, and O. Pavlíková. "Polyphenol content and antiradical activity in different apple varieties." Horticultural Science 33, No. 3 (November 23, 2011): 95–102. http://dx.doi.org/10.17221/3745-hortsci.
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Polyphenols are important antioxidant constituents of apples and they contribute positively to human health because they possess an antiradical activity. Fifteen apple varieties were analysed for their total polyphenol content (TP) by two methods – by Folin-Ciocalteau reagent (FC) and by EBC method with carboxylmethylcellulose/sodium ethylendiamintetraacetate (CMC/EDTA) and their antiradical activity (ARA) by DPPH method using stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH·). TP and ARA were determined in freshly expressed apple juice and apples and obtained results were statistically evaluated. The differences between varieties were significant. The correlation between TP content determined by FC method determining all free aromatic hydroxyls and EBC method determining mainly ortho-aromatic hydroxyls was found with r = 0.73. The highest differences among analysed varieties were found for ARA values in both, juice and apples and for TP content determined by FC in apples. High polyphenol content was found in Jonagold, Jonalord, Melodie and Melrose varieties both, in apples and juices; on the contrary low TP contents were estimated in Gloster and Rosana varieties. The highest ARA levels were found inRajka,Bohemia andMelrose varieties, compared to low ARA levels found in apple fruits of Šampion and Topaz varieties.
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Kaminskiy, Il'ya Petrovich, Yelena Vasil'yevna Yermilova, Tat'yana Vladimirovna Kadyrova, Mariya Sergeyevna Lar'kina, Anton Aleksandrovich D'yakonov, and Mikhail Valer'yevich Belousov. "ANTIRADICAL ACTIVITY OF EXTRACTS FROM SIBERIAN FLORA GENUS CENTAUREA PLANTS." chemistry of plant raw material, no. 4 (December 27, 2019): 173–79. http://dx.doi.org/10.14258/jcprm.2019045409.
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The purpose of this research is to study the antiradical activity of various extracts from the Centaurea scabiosa L. aerial part (wild-growing and cultivated) and Centaurea jacea L. as potential sources of antioxidant herbal remedies.
In the study of the antiradical activity of extracts from the aerial part of Centaurea jacea L., Centaurea scabiosa L. wild growing and cultivated, in the reaction with a stable diphenylpicrylhydrazyl radical, it was found that the degree of antiradical activity within each species correlates with the quantitative content of flavonoids. Lower values of the antiradical activity of cultivated Centaurea scabiosa L. extracts compared to wild growing Centaurea scabiosa L. extracts are consistent with a lower content of flavonoids in the latter.
The dependence of the Centaurea jacea L. and wild growing Centaurea scabiosa L. extracts antiradical activity on flavonoids quantitative and qualitative composition of these species has been established.
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Zykova, Irina Dement'yevna, Aleksandr Alekseyevich Efremov, and Liliya Viktorovna Naimushina. "ANTIRADICAL ACTIVITY OF SEPARATE FRACTIONS OF ESSENTIAL OIL OF ACORUS CALAMUS ROOTS." chemistry of plant raw material, no. 2 (June 10, 2020): 73–78. http://dx.doi.org/10.14258/jcprm.2020026659.
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Essential oil from the roots of calamus marsh (Acorus calamus L) was isolated by the method of exhaustive hydroparodistillation, growing on the Krasnoyarsk territory. Separate fractions of essential oil were obtained depending on the duration of isolation. The component composition of the essential oil, the main components of which are acorenone, butyrate α-terpineol, germacrene B, camphor. The antiradical properties of the essential oil of A. сalamus roots growing in the Siberian region, as well as the antiradical properties of its individual fractions were studied for the first time in order to determine the most promising samples as sources of biologically active complexes. To determine the antiradical activity, the reaction of the essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. The antiradical activity of all studied samples of essential oils was established. There was an increase in antiradical activity with a decrease in the content of monoterpenes in the composition of the essential oil. The lowest antiradical activity (17.5%) was shown by the fraction of essential oil with a high content of monoterpenes, the highest (~100%) – fractions with a high content of oxygen-containing compounds. It was revealed that the ability of components of individual fractions of A. сalamus essential oil to inhibit DPPH radicals is comparable with the antiradical activity of ascorbic acid solutions of equivalent concentration.
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L. B. IRISKINA ,, SH. А. МUZDYBAYEVA ,, М. К. ZHAMANBAEVA ,, A. SH. BUKUNOVA,, and G. К. DAUMOVA. "STUDYING OF ANTI-RADICAL PROPERTIES OF PHENOLS AND HETEROCYCLIC AMINES." Bulletin of the National Engineering Academy of the Republic of Kazakhstan 3, no. 77 (October 15, 2020): 119–25. http://dx.doi.org/10.47533/2020.1606-146x.18.
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This work presents the results of studies on the antiradical activity of phenols and heterocyclic amines. The antiradical activity was studied using the reaction of interaction with a stable colored radical by spectrophotometric method. The antiradical activity of natural polyphenol quercetin was studied. The content of quercetin in the dosage form was determined. It was shown that the studied phenols are medium strength inhibitors, and heterocyclic amines exhibit weak inhibitory activity.
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K. Singh, Anil, and Solomon B. Libsu. "Antiradical Activity of β-Ionyl Compounds." Letters in Organic Chemistry 7, no. 4 (June 1, 2010): 338–42. http://dx.doi.org/10.2174/157017810791130603.
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Yashin, Alexander, Yakov Yashin, Jing Wang, and Boris Nemzer. "Antioxidant and Antiradical Activity of Coffee." Antioxidants 2, no. 4 (October 15, 2013): 230–45. http://dx.doi.org/10.3390/antiox2040230.
Full textDissertations / Theses on the topic "Antiradical activity"
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Šlajūtė, Jolanta. "Lietuvoje augančių gervuogių vaisių fitocheminės sudėties įvairovės tyrimas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140630_134001-41351.
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Šio tyrimo tikslas yra įvertinti gervuogių (Rubus caesius L. ir Rubus nessensis Hall.) vaisių antocianinų sudėtį ir jos įvairavimą skirtingu nokimo metu bei vaisių ekstraktų antiradikalinį aktyvumą.
Vienas iš tyrimo objektų yra dviejų skirtingų Rubus genties atstovų (Rubus caesius L. ir Rubus nessensis Hall.) vaisiai, kurie buvo renkami periodiškai kas savaitę nuo pat vaisių derėjimo pradžios, Šilutės rajone. Nustačius optimalias ekstrahavimo sąlygas, vaisių etanoliniai ekstraktai buvo tiriami spektrofometrijos metodu bei nustatomas ekstraktų antiradikalinis aktyvumas, atliekant fotometrinius 2,2-difenil-1-pikrilhidrazilo (DPPH) bei 2,2’-azino-bis-(3-etilbenztiazolin-6-sulfono rūgšties) (ABTS) radikalo katijono sujungimo metodus, o gauti rezultatai vertinami remiantis pagal tai, kurią nokimo savaitę buvo renkami vaisių mėginiai.
Antrasis tyrimo objektas – R.caesius L. vaisiai, surinkti iš įvairių Lietuvos vietovių, kurių etanolinių ekstraktų sudėtyje esantis antocianinų suminis kiekis taip pat buvo vertinamas spektrofotometrijos metodu, tačiau šioje tyrimo dalyje buvo siekiama nustatyti antocianinų sudėties įvairavimą priklausomai nuo gervuogių vaisių rinkimo vietovės.
Tyrimo rezultatai. Nustatyta, jog iš dviejų tirtų gervuogių rūšių, gausesne antocianinų frakcijos sudėtimi pasižymi R. caesius L. vaisių ekstraktai, kuriuose antocianinų kiekis siekė nuo 4,16 proc. iki 4,89 proc. sausoje žaliavos masėje. Atitinkamai R. nessensis Hall. vaisių ekstraktuose nustatyta antocianinų... [toliau žr. visą tekstą]The objective of this study is to evaluate the blackberry (Rubus caesius L and Rubus nessensis Hall.) fruit’s anthocyanin composition and its variation during different ripening time and also to measure the antiradical activity in fruit extracts. One of the objects of the study is two different Rubus genus (Rubus caesius L. and Rubus nessensis Hall.) fruits, which were collected weekly from the beginning of fruit bearing in Šilutė’s area. Once the optimum extraction conditions were determined, the ethanol extracts of the fruits have been investigated using spectrophotometry and their antiradical activity was investigated using photometric 2,2- diphenyl-1-picrylhydrazyl (DPPH) and 2,2 '-azine-bis-(3- ethylbenzthiazoline-6-sulfonic acid ) (ABTS) radical scavenging techniques, and the results were evaluated according to the time, when fruit samples were collected. The second object of the research - R.caesius L. fruits collected from different locations in Lithuania. The total amount of anthocyanins in the extracts was also determined using spectrophotometry, but in this part of the study the main point was to determine how the variation of anthocyanins depends on the blackberry fruit collection area. The results showed that from two studied species of blackberry, R. caesius L. fruit extracts contained a larger amount of anthocyanin’s fraction, compared to R. nessensis Hall. fruits. In R. caesius L. fruit extracts anthocyanin’s concentration ranged from 4,16 percent to 4.89... [to full text]
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Godinho, Irina Sofia Marques. "Production of fish protein hydrolysates by a marine proteolytic strain." Master's thesis, ISA, 2013. http://hdl.handle.net/10400.5/6475.
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Mestrado em Engenharia Alimentar - Instituto Superior de AgronomiaThe preparation of fish protein hydrolysates by a fermentative process is an alternative to classical preparation using added proteolytic enzymes, taking advantage of the wide spectrum of proteolytic enzymes produced by microorganisms and increasing the complexity of the peptide mixture obtained. Thus, the objective of this work was to prepare fermentative protein hydrolysates (FPH) from a commercial cod protein hydrolysate (CPH) using an indigenous marine proteolytic strain. The bacterial growth was performed in liquid culture media, containing three concentrations of CPH (1, 2 and 4%), yeast extract (0.5%) and sucrose (0.5%), with continuous shaking, at 30 ºC for 24, 50 and 72 hours. The parameters evaluated were the culture growth, enzymatic activity, protein content and recovery, peptide profile, degree of hydrolysis and antioxidant activity, measured by DPPH and ABTS radical scavenging activity, reducing power and metal chelating activity. Bacterial growth and enzymatic activity reached their maximums after 24 hours of fermentation, and all FPH showed the disappearance of peptides in the range of 200 – 400 Da. In the set of samples, 4 % FPH recorded the highest values of protein content and recovery, but 1 % and 2 % FPH were the samples that presented improved antioxidant activity.
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Yagi, Sakina. "Etudes phytochimique et biologique de plantes soudanaises : Hydnora johannis Beccari (Hydnoraceae) et Citrullus lanatus (Thunb.) Matsum. et Nakai var. citroides (Bailey) Mansf. (Cucurbitaceae)." Thesis, Nancy 1, 2011. http://www.theses.fr/2011NAN10138.
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Différents extraits ont été préparés à partir de racines de H. johannis et différents tests biologiques ont été appliqués en vue de rechercher différentes activités. L'extrait aqueux s'est montré particulièrement actif sur Enterococcus fecalis, Staphylococcus aureus et Bacillus. Les extraits aqueux dépourvus de tanins et les tanins isolés ne présentent pas d'activité antibactérienne. L'effet synergétique des composés serait donc responsable de l'activité antibactérienne de la plante. Une activité antifongique sur Microsporum canis, une propriété antiradicalaire et une activité antiglycation ont été constatées avec les deux extraits. Une étude toxicologique de la poudre de plante et de l'extrait éthanolique sur des rats révèle une toxicité au niveau du foie et de la rate. Cinq composés ont été isolés puis identifiés. Il s'agit de 3',4',5-Trihydroxy-6,7-diméthoxyflavone ; 3,5-Dihydroxy-4,7-diméthoxy dihydroflavonol, Catéchine, Vanilline et l'acide Protocatechuic. Du stigmastérol, de l'acide oléique, de l'acide myristique et de l'acide palmitique ont été également identifiés. Le travail sur C. lanatus var. citroides a montré que l'extrait méthanolique (70%) des pulpes de fruits possède une activité contre B. subtilis, S. aureus et E. coli. Les extraits butanolique et à l'acétate d'éthyle ne sont pas toxiques contre les larves de crevettes. L'extrait butanolique possède une propriété significative antiradicalaire. Deux composés ont été isolés et identifiés. Ce sont la Cucurbitacine E 2-O-[bêta]-glucopyranoside et la Cucurbitacine L 2-O-[bêta]-glucopyranoside. Ces composés montrent une activité antibacterienne contre E. coli. La Cucurbitacine L 2-O-[bêta]-glucopyranoside possède une activité antibactérienne contre P. aeruginosa et une propriété modérée anti-radicaux libresDifferent extracts were prepared from the roots of H. johannis and different biological tests were performed. Water extract exhibited significant activity against Enterococcus fecalis, Staphylococcus aureus and Bacillus. Water extract devoid from tannin or the tannin fraction did not show any antibacterial activity reflecting the synergistic property of active compounds. Both extracts showed antifungal, antiradical capacity as well as antiglycation activity. Toxicological study of the powder and ethanol extract on rats showed toxicity to the liver and kidney tissues. Five compounds were isolated namely; 3,4,5- Trihydroxy- 6,7-dimethoxy flavone ; 3,5-Dihydroxy- 4,7- dimethoxy dihydroflavonol, Catechin, Vanillin and Protocatechuic acid. Stigmasterol, Oleic acid, Myristic acid and Palmitic acid were also identified. A study on the fruit pulps of C. lanatus var. citroides revealed that the methanolic extract displayed an antibacterial activity against B. subtilis, S. aureus and E. coli. The butanolic extract showed antiradical capacity and was not toxic to brine shrimps larvae. Two compounds were isolated namely; Cucurbitacine E 2-O-[bêta]-glucopyranoside and Cucurbitacine L 2-O- [bêta] -glucopyranoside. Both compounds showed antibacterial activity against E.coli whereas, Cucurbitacine L 2-O-[bêta]-glucopyranoside showed antibacterial activity against P. aeruginosa as well as antiradical activity
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Tamayose, Cinthia Indy. "Determinação da atividade antirradicalar e da constituição química de infusões de chás." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-21012015-085803/.
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O chá obtido por infusão de Camellia sinensis (L.) O. Kuntze (Theaceae) contém polifenóis, principalmente catequinas e flavonóis que apresentam atividade antioxidante, atuando como sequestradores de íons metálicos ou pelo sequestro de espécies reativas de oxigênio ou de nitrogênio. A erva-mate utilizada para o preparo da bebida chimarrão é feita a partir das folhas da arvore Ilex paraguariensis (Aquifoliaceae). A bebida da erva-mate é reconhecida como uma rica fonte de substâncias antioxidante, como os ácidos fenólicos que são responsáveis pelo efeito antioxidante in vitro e in vivo da bebida. Neste trabalho foi determinada a atividade antirradicalar de infusões obtidas de diferentes chás comerciais, o chá verde orgânico (CVorg) e um composto comercial de erva mate com chá verde (M+V). Os principais constituintes químicos dos chás foram identificados por Cromatografia de Alta Eficiência (CLAE) e alguns dos constituintes foram quantificados. Dessa forma foi possível identificar em ambos os chás ao todo cinco flavonoides glicosilados, cinco ácidos clorogênicos, cinco catequinas e um alcaloide. As infusões e algumas das substâncias identificadas foram avaliadas em relação à atividade antirradicalar utilizando diferentes métodos, um colorimétrico com os radicais 2,2-difenil-1-picrilhidrazila (DPPH·) e ácido 2,2\'-azino-bis(3-etilbenzotiazolina-6-sulfônico) (ABTS·+) e um método baseado na quimiluminescência do luminol. A infusão do CVorg mostra capacidade antirradicalar elevada quando o método com o radical ABTS·+ é utilizado, com valores similares aos obtidos com o padrão trolox®. Quando dois derivados catequinas foram submetidas ao mesmo ensaio com os dois radicais, observou-se que estes apresentaram uma capacidade antirradicalar maior frente ao radical ABTS·+, sugerindo que a elevada atividade antirradicalar da infusão CVorg pode ser atribuída à presença das catequinas analisadas. No método quimiluminescente a infusão do M+V apresentou uma capacidade antirradicalar mais alta que a infusão do CVorg. O ácido 5-cafeoilquinico, um derivado do ácido clorogênico, testado no mesmo ensaio, apresentou um potencial antirradicalar maior que as catequinas. Este resultado pode sugerir que a maior capacidade antirradicalar da infusão M+V comparado com a de CVorg, quando determinada com o ensaio luminol, pode ser atribuída à presença dos derivados do ácido clorogênico, tendo em vista que esta classe de compostos não foi identificada na infusão CVorg.Tea obtained by infusion of Camellia sinensis (L.) O. Kuntze (Theaceae) contains polyphenols, especially catechins and flavonols which exhibit antioxidant activity, acting as scavengers of metal ions or by sequestering reactive oxygen and nitrogen species. The mate herb used for brewing the mate beverage \'erva-mate\' is made from the leaves of the tree Ilex paraguariensis (Aquifoliaceae). The beverage \'erva-mate\' is known as a rich source of antioxidant substances, such as phenolic acids that are responsible for the in vitro and in vivo antioxidant effect of the beverage. In this work the antiradical activity of infusions obtained from different commercial teas, organic green tea (CVorg) and a commercial mixture of mate herb and green tea (M + V) was determined. The main chemical constituents of the teas were identified by High Performance Liquid Chromatography (HPLC) and some of the constituents quantified. Thus it was possible to identify in both teas a total number of five glycosylated flavonoids, five chlorogenic acids derivatives, five catechins and one alkaloid. The infusions and some of the identified constituents were evaluated for its antiradical activity using different methods, a colorimetric one with the stable radicals 2,2-diphenyl-1-picrylhidrazyl (DPPH·) and 2,2\'-azino-bis(3-ethylbenzothiazoline-6-sulfonico acid (ABTS·+) and an alternative method based on luminol chemiluminescence. The infusion of CVorg shows high antiradical capacity when evaluated by the method with the radical ABTS·+, with values similar to that of the standard trolox®. When two catechins derivatives were subjected to the same test with both radical, it was observed that these showed higher antiradical capacity with the radical ABTS·+, as compared to DPPH·, suggesting that the high antiradical capacity of the CVorg infusion can be attributed to the presence of analyzed catechins. With the chemiluminescence method the M+V infusion showed a higher antiradical capacity that the CVorg infusion. 5-Cafeoilquinic acid, a chlorogenic acid derivative, tested in this assay showed a higher antiradical capacity than catechins. This result might suggest that the higher antiradical capacity of the M+V infusion as compared to the CVorg, when measured with the luminol method, can be attributed to the presence of chlorogenic acid derivatives, since this class of compounds was not identified in the CVorg infusion.
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SILVA, Paulo Ricardo da. "Estudo químico e potencial antioxidante do mel e geoprópolis coletados pela abelha sem ferrão mandaçaia (Melipona mandacaia)." Universidade Federal Rural de Pernambuco, 2015. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6715.
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] The species of stingless bee Melipona mandacaia is popularly known as mandaçaia and is native to northeastern Brazil. This study analyzed honey and geopropolis of mandaçaia. For honey were carried out pollen, physical and chemical analysis such as moisture, pH, free acidity, hydroxymethylfurfural, ash content, reducing sugars and water activity, amino acids, minerals and antiradical activity of four samples of honey. The major phenolic constituents of honey were extracted and analyzed by High Performance Liquid Chromatography Efficiency coupled to the Diode Array Detector (HPLC-DAD). Pollen analysis showed that the dominant pollen in honey samples was the plant species Mimosa arenosa (Fabaceae / Mimosoideae) ranging from 44.4% to 61.7%. The identified flavonoids quercetin, luteolin, and kaempferol acid derivatives: 3,4-dihydroxybenzoic, 1,2-dihydroxybenzoic, caffeic, cinnamic and ferulic were quantified. All honey samples showed the amino acid proline, alanine, serine and threonine. The minerals were the predominant calcium and potassium. All honeys showed scavenging activity of free radical front to DPPH and ABTS. For geopropolis of mandaçaia was determined to botanical origin, phenolics were quantified by HPLC-DAD and the antioxidant activity was determined by testing with DPPH, ABTS and beta carotene / linoleic acid system. Pollen analysis of nine samples geopropolis showed the presence of 25 types of pollen representing fifteen families. The Senna species (Leguminoseae) was the predominant pollen in eight of the nine samples. The phenol content in the geopropolis of mandaçaia were identified as caffeic acid, coumaric acid, trans-3-hydroxycinnamic acid, 4-methoxycinnamic acid and abscisic. All samples geopropolis showed antioxidant activity, except hexanic fractions that have been inactive for the free radical DPPH.
A espécie de abelha sem ferrão Melipona mandacaia é conhecida popularmente como mandaçaia e é nativa do Nordeste brasileiro. Neste estudo foi analisado o mel e a geoprópolis da mandaçaia. Foram realizadas as análises palinológicas, físico-químicas tais como umidade, pH, acidez livre, hidroximetilfurfural, teor de cinzas, açucares redutores e atividade de água, aminoácidos, minerais e atividade antirradicalar em quatro amostras de mel. Os principais constituintes fenólicos do mel foram extraídos e analisados por Cromatografia Líquida de Alta Eficiência acoplada ao Detector de Arranjo de Diodos (CLAE-DAD). A análise palinológica mostrou que o pólen predominante nas amostras de mel foi da espécie vegetal Mimosa arenosa (Fabaceae/Mimosoideae), variando de 44,4% a 61,7%. Os flavonoides identificados quercetina, luteolina, kampferol e os derivados de ácido: 3,4-dihidroxibenzoico, 1,2-dihidroxibenzoico, cafeico, cinâmico e ferúlico foram quantificados. Todas as amostras de mel apresentaram os aminoácidos prolina, alanina, serina e treonina. Os minerais predominantes foram o cálcio e potássio. Todos os méis apresentaram atividade sequestradora de radical livre frente ao DPPH e ABTS. A origem botânica da geoprópolis da mandaçaia foi determinada e os compostos fenólicos foram quantificados por CLAE-DAD e a atividade antioxidante foi determinada pelos testes com DPPH, ABTS e o sistema beta caroteno/ácido linoleico. A análise palinológica das nove amostras de geoprópolis mostraram a presença de 25 tipos de pólen representando quinze famílias. O tipo polínico Senna (Leguminoseae) foi o predominante em oito das nove amostras analisadas. Os fenólicos presentes na geoprópolis da mandaçaia foram identificados como sendo ácidos cafeico, cumárico, trans-3-hidroxicinâmico, abscísico e 4-metoxicinâmico. Todas as amostras de geoprópolis apresentaram atividade antioxidante, exceto as frações hexânicas que foram inativas para o radical livre DPPH.
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Vitalini, S. "EFFECT OF SAR INDUCERS ON GRAPE SECONDARY METABOLITES." Doctoral thesis, Università degli Studi di Milano, 2012. http://hdl.handle.net/2434/168721.
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Fungicide application is now the most efficacious method for controlling plant diseases caused by oomycetes and fungi. As legislation is limiting and reducing their use, it is strongly stimulating studies for the identification of additional and environmentally friendly approaches in the control of their associated diseases. Among these, systemic acquired resistance (SAR) offers the prospect of long-lasting, broad-spectrum disease control through activation of the resistance defence machinery of the plant itself. Plant activators are products employed in crop protection able to elicit SAR. Therefore, they may trigger the plant own defence response against pathogen attacks, mainly stimulating mechanisms such as the biosynthesis of phytoalexins, plant secondary metabolites with a broad spectrum biological activity. In this study, two plant defence inducers, benzothiadiazole (BTH) and chitosan (CHT), deserving particular attention because of their efficacy and low toxicity, have been used. CHT is a natural and low-cost polymer (from the waste products of the crustacean carapace), obtained by chitin deacetylation. Its effectiveness is higher when molecular weight is between 10 and 100 kD and the deacetylation degree range is from 80 to 90 percent. BTH [benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester] is a synthetic compound and a functional analogue of salicylic acid, a plant hormone-like compound deeply involved in resistance against pathogens. Two different phytoiatric campaigns were planned, in 2009 and 2010, on two red grapevine (Vitis vinifera L.) varieties cultivated in experimental vineyards located at distinct sites: Groppello, an autochthonous cultivar of Lombardia, at Raffa di Puegnago (Brescia), and Merlot at Conegliano Veneto (Treviso). Open field treatments carried out on Groppello grapevines were: i) 0.03% (w/v) CHT (76 kDa molecular weight and 85% deacetylation degree), ii) 0.03% CHT in combination with 150 g hL-1 copper hydroxide (CHT/Cu) and iii) 0.3 mM BTH; whereas, on Merlot, besides CHT and CHT/Cu, BTH were replaced by 300 g hL-1 potassium phosphites. Untreated vines were used as negative control, while plants treated with conventional fungicides (penconazole and methyldinocap) were the positive control. In both field surveys, the trial was set up as a complete randomized block design in 4 replications, with 10 vines (a parcel) per treatment in each block. Plants were sprayed approximately every 10 days, according to the meteorological conditions, from the beginning of grape susceptibility to fungal diseases until the complete véraison. The phytosanitary status of vineyards was assessed weekly on leaves and bunches, by visual inspections, though both sites were not particularly predisposed to severe fungal or other epidemics. The epidemiological evaluations were performed on bunches alone and infection indexes were calculated. All the treatments were effective in controlling fungal infections (downy mildew, Plasmopara viticola and powdery mildew, Erysiphe necator), in terms of disease incidence (I %), disease severity (S %) and infection degree (ID %), though these indexes were low in untreated control grapevines, particularly in 2010. Sampling was scheduled at two phenological phases: pre-véraison and 100% véraison; bunches were randomly collected from plants during the morning and stored at -20 °C until analyses. Berry tissues were separated into skin, flesh and seeds, powdered and extracted. Then, extracts were tested to evaluate their melatonin levels [by ultra performance liquid chromatography (UPLC)-MS/MS], total polyphenols (TP, by Folin-Ciocalteau assay) and antiradical activity [by DPPH, 2,2-diphenyl-1-pycryl hydrazyl and ABTS, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay]. Groppello and Merlot experimental wines were produced, by standard microvinification techniques, in the Centro Vitivinicolo Provinciale of Brescia and Centro di Ricerca per la Viticoltura of Conegliano (TV), respectively, and stored at 4 °C in the dark until analyses. Microvinificates were produced from grapes treated with both elicitors and conventional fungicides (controls), and the following analyses, besides those also conducted for all berry tissues, were carried out: tryptophan, serotonin and melatonin detection (by UPLC-MS/MS); content of cis and trans resveratrol (by UPLC-MS/MS); levels of mycotoxins (manly ochratoxin A, OTA, by UPLC-MS/MS). In general, berry tissues treated with elicitors and the corresponding experimental wines showed higher levels of melatonin and polyphenols, as well as a higher antiradical activity than samples treated with conventional fungicides. High concentrations of tryptophan were detected in all samples, contrary to serotonin, which was not detected. The most effective elictors were CHT/Cu and CHT. In all wines, the level of OTA was below the allowable threshold of 2 ng/mL. To the best of our knowledge, these results represent the first data on the effects of agrochemicals on the melatonin content of red wine, and the presence of melatonin was reported, for the first time, in berry seeds and flash, after its previously detection in skin. Furthermore, the level of the indolamine in berry tissues varies according to the phenological stage, resulting more abundant in seed at pre-véraison and in skin at véraison. The good agreement between the data obtained in 2009 and 2010 for Groppello cultivar, and, in 2009, between Groppello and Merlot varieties cultivated in different geographical areas, suggests that, in general, plant activators may improve some qualitative/healthy treats ascribed to red wine, though their efficacy in controlling grapevine fungal diseases should be better ascertained. Finally, the role of melatonin, a powerful antioxidant, in grapevine physiology is still somewhat obscure. It is possible that, at pre-véraison, when skin anthocyanic pigmentation is still lacking, this compound may defend berry from damage due to photooxidation or UV radiation, whereas, at véraison, the indolamine may protect the germ tissues, particularly rich in storage lipids and membranes and vulnerable to oxidative damage. In conclusion, the possibility of enhancing the pharmaconutritional potential of grape/wine with phytosanitary treatments should be further tajen into account.
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NASCIMENTO, Rosilda Josefa do. "Potencial antioxidante de resíduo agroindustrial de goiaba." Universidade Federal Rural de Pernambuco, 2010. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5098.
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Considering the high production of agri-industrial waste and the possibility of this material have bioactive phytochemicals, this work aimed to evaluate the antioxidant potential of the guava waste (seed) from an industry of frozen fruit pulps. Hydroacetone, hydroethanolic, hydromethanolic and aqueous extracts, obtained by sequential extraction procedure, were submitted to the determination of total phenolic and antioxidant activity in β-carotene/linoleic acid co-oxidation system and by ferric thiocyanate method and screened for their free [DPPH• (1,1-diphenyl-2-picrilhidrazil) and ABTS•+ (2,2'-azino-bis-(3- etilbenzotiazolina 6-sulfonic acid)] scavenging activity. The hydroacetone and hydromethanolic extracts exhibited the highest content of total phenolics (5317.27 and 2176.46 ag catechin equivalent mL-1, respectively), while the aqueous and hydroethanolic extracts were excluded from antioxidant analysis. Hydroacetone extract exhibited high percentage of the inhibition of oxidation (81.95%) in β-carotene/linoleic acid co-oxidation system; high percentage of the inhibition of linoleic acid peroxidation (74.32% and 80.13%at concentrations 140 and 240mg/L, respectively), good DPPH• scavenging activity (scavenging percentage >60%; low value of EC50 and TEC50, middle value of AE), and good ABTS•+ scavenging activity (875.79mM TEAC.g-1). The hydromethanolic extract exhibited low percentage of the inhibition of oxidation (38.92%) in β-carotene/linoleic acid co-oxidation system, inhibition of linoleic acid peroxidation of the 50.50% and 73.70% (at concentrations 140 and 240mg/L, respectively), DPPH• scavenging activity (scavenging percentage < 30%, high EC50 and TEC50, and low value of AE), and bad ABTS• + scavenging activity (57.16 mM TEAC.g -1). Combining of the hydroacetone extract with BHT at different concentrations, the scavenging activity and inhibition of oxidation, in β-carotene/linoleic acid co-oxidation system were similar to that BHT alone, which demonstrated synergism between them. Hydroacetone extract from guava waste (seeds) has a significant potential antioxidant since it has shown better performance in scavenging DPPH • and ABTS• + radical, important antioxidant activity in lipid emulsion system, and synergism with BHT. Thus, the agri-industrial waste from guava can be considered as alternative of natural antioxidants.
Diante da elevada produção de resíduo agroindustrial e da possibilidade deste material conter fitoquímicos bioativos, este trabalho objetivou avaliar o potencial antioxidante de resíduo de goiaba (semente) proveniente de uma indústria de polpas de frutas congeladas. Extratos hidroacetônico, hidrometanólico, hidroetanólico e aquoso, obtidos por extração sequencial, foram utilizados para quantificar o teor de fenólicos totais e determinar a atividade antioxidante em sistema da co-oxidação β-caroteno/ácido linoléico e pelo método tiocianato férrico e a capacidade de seqüestrar radicais livres [DPPH• (1,1- difenil-2-picrilhidrazil) e ABTS•+ (2,2’-azino-bis-(3-etilbenzotiazolina-6-ácido sulfônico]. Os extratos hidroacetônico e hidrometanólico exibiram os maiores teores de fenólicos totais (5.317,27 e 2.176,46ag em equivalente de catequina mL-1, respectivamente), sendo, portanto, o aquoso e hidroetanólico excluídos da avaliação do potencial antioxidante. O extrato hidroacetônico exibiu elevado percentual de inibição da oxidação (81,95%) no ensaio da co-oxidação do β-caroteno/ acido linoléico; elevado percentual de inibição da peroxidação do ácido linoléico (74,32% e 80,13%, respectivamente, nas concentraçõesde 140 e 240mg/L); boa capacidade de sequestrar o radical DPPH (percentual de sequestro >60%, baixo valor de EC50 e de TEC50, e médio EA); e o radical ABTS•+ (875,79 mM TEAC.g-1). O extrato hidrometanólico exibiu baixo percentual de inibição da oxidação (38,92%) no ensaio da co-oxidação do β-caroteno/ acido linoléico; inibição da peroxidação do ácido linoléico de 50,50% e 73,70% (nas concentrações de 140 e 240mg/L, respectivamente); e fraca capacidade de sequestrar o radical DPPH (percentual de sequestro < 30%, alto valor de EC50 e de TEC50, e baixo EA) e o radical ABTS•+(57,16 mM TEAC.g-1). Ao associar o extrato hidroacetônico com o BHT, em diferentes concentrações, o percentual de sequestro do DPPH e de inibição da oxidação, em sistema da co-oxidação β-caroteno/ácido linoléico foi semelhante ao do BHT isolado, demonstrando haver sinergismo entre eles. O extrato hidroacetônico do resíduo agroindustrial de goiaba apresenta um expressivo potencial anti-radical, uma vez que demonstrou eficiência na captura dos radicais DPPH• e ABTS•+, relevante ação antioxidante em meio lipídico, além de sinergismo com o BHT. Desta forma, o resíduo agroindustrial de goiaba surge como alternativa de antioxidante natural.
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Jasaitytė, Jolanta. "Propolio neetanolinio ekstrakto technologijos parinkimas ir veikliųjų medžiagų analizė." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215623-50977.
Full textAbstract:
Tyrimo tikslas - parinkti neetanolinio propolio ekstrakto technologiją ir atlikti veikliųjų medžiagų analizę.
Uždaviniai: nustatyti propolio koncentracijos, ekstrakcijos tirpiklių bei jų koncentracijų, ekstrakcijos laiko, temperatūros, ekstrakcijos metodo įtakas fenolinių junginių išsiskyrimui. Nustatyti propolio ekstrakto antiradikalinį aktyvumą ir flavonoidų kiekį bei efektyviosios skysčių chromatografijos metodu nustatyti propolio ekstrakte esančius cheminius junginius ir jų antiradikalinį aktyvumą.
Metodika. Bendras fenolinių junginių kiekis, buvo nustatytas naudojant farmakopėjinį Folino Ciocalteu metodą pagal galo rūgšties ekvivalentą. Flavonoidų kiekis nustatytas naudojant spektrofotometrinį metodą pagal rutino ekvivalentą. Antioksidacinis aktyvumas spektrofotometriškai buvo nustatomas naudojant DPPH radikalą. Efektyviosios skysčių chromatografijos su elektrocheminiu detektoriumi būdu buvo identifikuoti fenoliniai junginiai ir nustatytas jų antiradikalinis aktyvumas.
Rezultatai. Didinant propolio koncentraciją, naudojant tirpiklius didėja bendras fenolinių junginių kiekis (p<0,05). Technologiniai procesai t.y. ekstrakcijos trukmė, temperatūros padidinimas daro reikšmingą įtaką fenolinių junginių išsiskyrimui (p<0,05). Didžiausias bendras fenolinių junginių kiekis 35,60 mg/ml buvo gautas naudojant ultragarsu skatinamą ekstrakciją 10 min esant 70 ⁰C temperatūrai, ekstrakcijos tirpikliu naudojant 30 proc. makrogolio ir vandens mišinį. Šiame ekstrakte nustatytas 88,76±1... [toliau žr. visą tekstą]The aim of survey - to select the technology of nonethanolic propolis extract and analyse the active substances. Tasks: To examine the influence of concentration, solvent and its concentration, extraction time, temperature, the extraction method on release of total phenolic compounds. Determine the radical scavenging activity and flavonoid content of propolis extract and using high performance liquid chromatography method identify active substances and its antiradical activity. Methods: Total phenolic compounds were determined by using pharmacopoeial Folin Ciocalteu method expressed in gallic acid equivalents. Flavonoids were determined using a spectrophotometric method in rutin equivalent. Radical scavenging activity was determined by spectrophotometry using DPPH radical. High performance liquid chromatography with electrochemical detection were used to identify phenolic compounds and its radical scavenging activity. Results: Increasing of propolis concentration made a significance impact on the amount of total phenolic content (p<0,05). Extraction time, increase of temperature made a significance impact on increase of the amount of total phenolic content (p<0,05). The highest amount of total phenolic compounds 35,60 mg/ml were obtained using ultrasonic induced extraction for 10 minutes at 70 ⁰ C temperature and using 30 % macrogol – water solution as solvent. The radical scavenging capacity of this extract was 88,76 ± 1,27% and amount of flavonoids were 3,89 ± 0,43 mg/ml... [to full text]
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Ljiljana, Janjušević. "Biološka aktivnost i hemijski sastav ekstrakata odabranih autohtonih makrogljiva." Phd thesis, Univerzitet u Novom Sadu, Prirodno-matematički fakultet u Novom Sadu, 2017. https://www.cris.uns.ac.rs/record.jsf?recordId=104929&source=NDLTD&language=en.
Full textAbstract:
Prema postavljenim ciljevima u ovoj doktorskoj disertaciji sakupljeno je i determinisano ukupno sedam vrsta autohtonih gljiva sa područja Fruške gore, Tare i Vršačkog brega, pet lignikolnih ‐ Bjerkandera adusta, Pleurocybella porrigens, Stereum hirsutum, Stereum subtomentosum i Trametes versicolor, i dve terikolne ‐ Amanita strobiliformis i Hydnum repandum. Utvrđena je njihova biološka aktivnost (antiradikalska, antioksidativna, antimikrobna, anti‐acetilholinesterazna i citotokisčna) spram hemijskog sastava njihovih vodenih (H2O), etanolnih (EtOH), metanolnih (MeOH) i polisaharidnih (PSH) ekstrakata. Analiza hemijskog sastava odabranih vrsta uključila je određivanje hemijske karakterizacije PSH ekstrakata ‐ FTIR analizom, određivanje fenolnog profila ‐ HPMC/MS‐MS, sadržaja organskih kiselina ‐ HPLC, sadržaja masnih kiselina ‐ GC‐FID i sadržaja biogenih elemenata ‐ AAS. Spektrofotometrijskim metodamaodređen je ukupan sadržaj proteina i ukupan sadržaj fenola i flavonoida. Prema antiradikalskoj aktivnosti OH• , O2•‐, OH•, Asc•, DPPH• i ABTS•+ izdvojili su se ekstrakti lignikolnih vrsta: MeOH ekstrakt vrste P. porrigens, H2O ekstrakt P. porrigens, MeOH ekstrakt T. versicolor, H2O ekstrakt S. hirsutum, MeOH ekstrakt S. subtomentosum i H2O ekstrakt B. adusta, navedenim redom. Najjaču antioksidativnu aktivnost dobijenu FRAP i polarografskom HPMC metodom ispoljili su PSH i H2O ekstrakti terikolne vrste A. strobiliformis. Antimikrobna aktivnost analiziranih ekstrakata određena je ispitivanjem antibakterijskog, antifungalnog i antiviralnog potencijala, pri čemu se izdvojila vrsta H. repandum ispoljavajući najbolji efekat na Gram‐pozitivne i Gram‐negativne bakterije i na sve analizirane fitopatogene izolate (Fusarium i Alternaria) i T. versicolor na analizirani bakteriofag. Anti‐acetilholinesterazna aktivnost određena je testovima in solid i in liquid, a najbolji procenat inhibicije AChE ispoljili su EtOH ekstrakti vrsta S. hirsutum, B. adusta, S. subtomentosum i T. versicolor. Citotoksična aktivnost ekstrakata određena je MTT testom, a prema najboljoj ispoljenoj aktivnosti izdvojili su se MeOH ekstrakt P. porrigens i ekstrakti B. adusta, H2O i EtOH. Citotoksična aktivnost ovih lignikolnih vrsta naročito je izražena nakon 72 h. Na osnovu dobijenih rezultata, gde su se istakle različite vrste i njihovi različiti ekstrakti u primenjenim testovima, jasno je da biološka aktivnost i hemijski sastav zavise od porekla, vrste i tipa ekstrakta analiziranih gljiva. Na osnovu tipa rastvarača odnosno ekstrakata vrsta, koje su pokazale najbolju aktivnost spram pomenutih testova i na osnovu dobijenih korelacija kao i na osnovu detektovanih jedinjenja, pretpostavljamo da su za ispoljene aktivnosti u najvećoj meri odgovorna fenolna jedinjenja i polisaharidi. Ispoljeni biopotencijal analiziranih vrsta gljiva upućuje na njihovu potencijalnu upotrebu kao funkcionalne hrane i nutraceutika, kao i u biokontroli fitopatogena.According to the set aims of the presented PhD thesis, seven autochthonous fungal species from the region of Fruska Gora, Tara and Vršac Mountains were collected and determined: five lignicolous ‐ Bjerkandera adusta, Pleurocybella porrigens, Stereum hirsutum, Stereum subtomentosum and Trametes versicolor, and two terricolous ‐ Amanita strobiliformis and Hydnum repandum. Biological activity of these species (antiradical, antioxidant, antimicrobial, anti‐ acetylcholinesterase and cytotoxic) was determined in relation to the chemical composition of the extracts, aqueous (H2O), ethanolic (EtOH), methanolic (MeOH) and polysaccharide (PSH). Analysis of the chemical content of analyzed species included chemical characterization of PSH extracts – by FTIR analysis, determination of phenolic profile ‐ by HPMC/MS‐MS, content of organic acids ‐ by HPLC, fatty acid content ‐ by GC‐FID and content of biogenic elements ‐ by AAS. Spectrophotometric methods were applied for determination of the content of total proteins, polyphenols and flavonoids. According to the antiradical activity obtained towards OH•, О2•‐, OH•, Asc•, DPPH• and ABTS•+ extracts of lignicolous species were singled out: P. porrigens (MeOH extract), P. porrigens (H2O extract), T. versicolor (MeOH extract), S. hirsutum (H2O extract), S. subtomentosum (MeOH extract) and B. austa (H2O extract), respectively. The highest antioxidant activity obtained by FRAP and the polarographic HPMC method was exhibited for PSH and H2O extracts of the terricolous species A. strobiliformis. The intimicrobial activity of analyzed extracts was determined by examination of antibacterial, antifungal and antiviral potentials, whereby the species H. repandum was separated by exhibiting the best effect on Gram‐positive and Gram‐negative bacteria, and all the analyzed hytopathogenic isolates (Fusarium, Alternaria) and T. versicolor against analyzed bacteriophage. Anti‐cetylcholinesterase activity was determined by tests in solid and in liquid, while the best percent of AChE inhibition was showed by EtOH extracts of the species S. hirsutum, B. adusta, S. subtomentosum and T. versicolor.bThe cytotoxic activity of extracts was determined by MTT assay, and according to the best activity, the MeOH extract of P. porrigens, and H2O and EtOH extracts of B. adusta were distinguished particularly after 72 h. Based on the results obtained, favoring different species and their different extracts in the applied tests, it is clear that the biological activity and chemical composition depend on the origin, species and type of extract of the analyzed fungi. Based on the type of solvent or extract of the species that showed the best activity in relation to the above tests and on the basis of the obtained correlations as well as on the basis of the detected compounds, we assume that the phenol compounds and polysaccharides are responsible for the activities performed.Demonstrated bio‐potential of analyzed fungal species indicates their potential use as functional foods and nutraceutics, as well as in the biocontrol of phytopathogens.
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Jelena, Vulić. "Funkcionalne i antioksidativne osobine tropa cvekle (Beta vulgaris)." Phd thesis, Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad, 2012. https://www.cris.uns.ac.rs/record.jsf?recordId=77524&source=NDLTD&language=en.
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Etanolni ekstrakti tropa odabranih sorti cvekle (Detroit, Cardeal-F1, Egipatska, Bikor i Kestrel) prečišćeni su primenom ekstrakcije na čvrstoj fazi (SPE). Sadržaj ukupnihfenolnih jedinjenja, flavonoida i betalaina u prečišćenim ekstraktima određeni su spektrofotometrijskim metodama. HPLC analizom utvrđen je kvalitativni i kvantitativni sastav fenolnih jedinjenja i betalaina ekstrakata tropa odabranih sorti cvekle. ESR spektroskopijom ispitana je antiradikalska aktivnost ekstrakata topa cvekle na stabilne DPPH i reaktivne superoksid anjon i hidroksil radikale.Spektrofotometrijski je određena antioksidativna aktivnost na DPPH radikale i redukciona sposobnost po Oyaizu u ekstraktima odabranih sorti cvekle. Ispitana je in vitroantiproliferativna aktivnost frakcija ekstrakata, njihovim delovanjem na rast tri histološki različite humane ćelijske linije: MCF-7 (adenokarcinom dojke), HeLa (epitelni karcinom cerviksa)i MRC-5 (fetalni fibroblastni karcinom pluća). U završnoj fazi rada određena je antimikrobna aktivnost ekstrakata tropa odabranih sorti cvekle.Beetroot (Detroit, Cardeal-F1, Egipatska, Bikor i Kestrel) pomace ethanol extracts were purified using solid phase extraction (SPE). Contents of total phenols, flavonoids and betalains in purified extracts were determined by spectrophotometric methods. HPLC analysis were used for quantitative and qualitative characterization of phenolic compounds and betalains in investigated extracts. ESR spectroscopy was used for investigation of antiradical activity of beetroot pomace extracts on stable DPPH and reactive superoxide anion and hydroxyl radicals. Antioxidant activity was determined spectrophotometrically on DPPH radicals and reducing power according to Oyaizu in the beetroot pomace extracts. Antiproliferative activity of investigated extracts was determined in vitro, testing their influence on the growth of three histologically different human cell lines: MCF-7 (breast adenocarcinoma), HeLa (cervix epithelioid carcinoma) and MRC-5 (fetal lung). Also, antimicrobial activity of beetroot pomace extracts was determined.
Book chapters on the topic "Antiradical activity"
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Vinduja, Puttanveedu, and Karuvanthodi Muraleedharan. "Theoretical Studies on Anti-Oxidant Activity of the Phytochemical, Coumestrol and Its Derivatives." In Functional Foods - Phytochemicals and Health Promoting Potential. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96967.
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Free radical-induced changes in cellular and organ levels have been studied as a possible underlying cause of various adverse health conditions. Important research efforts have, therefore, been made to discover more powerful and potent antioxidants/free radical scavengers for the treatment of these adverse conditions. The phytoestrogen coumestrol intensively attracted scientific interest due to their efficient pharmacological activities. In this scenario, DFT studies were carried out to test the antiradical activities of coumestrol and its derivatives. The results obtained from FEDAM plots demonstrated that the coumestrol derivatives pointed out were good radical scavengers relative to the parent molecule in the gas phase. The derivatives whose 16thposition substituted with electron-donating groups like -NH2, -OCH3 and -CH3 showed good antioxidant capacity. Three antioxidant mechanisms, including hydrogen atom transfer (HAT), electron transfer followed by proton transfer (SET-PT), and sequential proton loss electron transfer (SPLET), were investigated by measuring thermodynamic parameters.
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"THE KINETIC DPPH-METHOD OF ANTIRADICAL ACTIVITY ANALYSIS OF THE MATERIALS OF PLANT ORIGIN." In Analysis and Performance of Engineering Materials, 425–42. Apple Academic Press, 2015. http://dx.doi.org/10.1201/b18864-20.
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Punetha, Himanshu, Satendra Kumar, Harish Mudila, and Om Prakash. "Brassica Meal as Source of Health Protecting Neuraceutical and Its Antioxidative Properties." In Complementary and Alternative Medicine, 260–83. IGI Global, 2019. http://dx.doi.org/10.4018/978-1-5225-7039-4.ch011.
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Rapeseed-mustard belongs to the Brassicaceae family and is an important group of oilseed crops, used as spices, medicine and as a source of edible oil since ancient times. Brassica seed meal could be highly regarded as an important component of human nutrition and animal feed due to its good protein content (45-48%), desirable amino acid profile rich in lysine with adequate amount of sulphur containing amino acids and low antinutrient content. The defatted meal is a rich in bioactive principles responsible for health protection such as tocopherol, glucosinolates, phytic acid, carotenoides, folic acid, ascorbic acid, polyphenols and flavonols. The seed meal is characterized by appreciable antiradical activity along with better meal stability properties. The antioxidative properties of edible portions of Brassica are due polyphenolic components. The present review focused on literature available from the neutaceutical component of Brassica meal and its antioxdative properties.
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Omedine, Koukoui Semako, and Agbangnan Cokou Pascal. "Antiradical Activity of Five Plants Used for the Treatment of Type 2 Diabetes in Benin: A Comparative and Analytical Overview." In Current Overview on Pharmaceutical Science Vol. 1, 27–43. B P International (a part of SCIENCEDOMAIN International), 2022. http://dx.doi.org/10.9734/bpi/cops/v1/4137e.
Full textConference papers on the topic "Antiradical activity"
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Keke, Anete, and Ingmars Cinkmanis. "Total phenolic content and antioxidant activity of honey powders." In Research for Rural Development 2021 : annual 27th International scientific conference proceedings. Latvia University of Life Sciences and Technologies, 2021. http://dx.doi.org/10.22616/rrd.27.2021.015.
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Honey powders could be an innovative and attractive substitutes to liquid honey. The production of honey powders would allow to expand honey’s usage in food industry, cosmetics and pharmaceutics. In this research, freeze drying method was performed to obtain honey powder samples. Overall, 4 different formulations were prepared to obtain the powder samples. The aim of the research was to determine the total phenolic content and antiradical activity of the freeze-dried honey powder samples by spectrophotometric method. The content of total phenolics in the samples ranged from 92 to 146 mg GAE 100 g-1 of dry matter. Antiradical activity was measured with DPPH radical scavengers, and the IC50 results ranged from 6 to 12 mg mL-1.
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Milanović, Žiko, Edina Avdović, Marko Antonijević, and Zoran Marković. "DIRECT SCAVENGING ACTIVITY OF 4,7-DIHYDROXYCOUMARIN DERIVATIVE TOWARDS SERIES OF CHLOROMETHYLPEROXY RADICALS." In 1st INTERNATIONAL Conference on Chemo and BioInformatics. Institute for Information Technologies, University of Kragujevac,, 2021. http://dx.doi.org/10.46793/iccbi21.462m.
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In this study, the mechanisms of antiradical activity of the previously synthesized 4,7- dihydroxycoumarin derivative:(E)-3-(1-((4-hydroxyphenyl)amino)ethylidene)-2,4- dioxochroman-7-yl acetate (A-4OH) according to the series of chloromethylperoxy radicals: OOCH2Cl (chloromethylperoxy), OOCHCl2 (dichloromethylperoxy) and OOCCl3 (trichloromethyl peroxy) were investigated. To assess the mechanisms of antiradical activity of A-4OH against chloromethylperoxy radicals, the following mechanistic pathways were examined: Hydrogen Atom Transfer (HAT), Single-Electron Transfer followed by Proton Transfer (SET-PT), and Radical Adduct Formation (RAF). The investigations were performed in water using sophisticated computational methods. The results of the study show that the activity against selected radicals decreases in series •OOCCl3 > •OOCHCl2 > •OOCH2Cl. The results of thermodynamic and kinetic parameters indicate that A-4OH inactivates selected radicals via the HAT mechanism.
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Milanovic, Žiko, Ana Kesic, Edina Avdovic, Jelena Đorovic Jovanovic, and Dejan Milenkovic. "UTICAJ pH VREDNOSTI NA ANTIRADIKALSKI KAPACITET 4,7- DIHIDROKSIKUMARINA." In XXVI savetovanje o biotehnologiji sa međunarodnim učešćem. University of Kragujevac, Faculty of Agronomy, 2021. http://dx.doi.org/10.46793/sbt26.481m.
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The mechanisms of antiradical activity of 4,7-dihydroxycoumarin against peroxy (HOO•) radical were investigated. For this purpose were used sophisticated computational methods. Investigation was performed in the water, at different pH values (0-14). Acid-base species, which are represented in diverse molar fraction (f) at different pH values, contribute to a comprehensive examination of antiradical capacity. An overall rate constant (koverall) of the favorable reaction pathways (HAT and RAF) of 2.07×102 M-1s-1 at physiological pH indicates a moderate ability to neutralization HOO• radicals. A value of 1.05×103 M-1 s-1 occurring at pH > 10 indicates a maximum antiradical capacity of 4,7-dihydroxymumarin against the HOO• radical.
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Frolova, M. A., E. I. Zelinskaya, A. I. Albulov, and A. K. Eliseev. "ANTIRADICAL AND SORPTION ACTIVITY OF MELANIN FROM THE BLACK SILION HERMETIA ILLUCENS FLY." In НАУЧНЫЕ ОСНОВЫ ПРОИЗВОДСТВА И ОБЕСПЕЧЕНИЯ КАЧЕСТВА БИОЛОГИЧЕСКИХ ПРЕПАРАТОВ. Лосино-Петровский: Б. и., 2022. http://dx.doi.org/10.47804/9785899040313_2022_212.
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Nikolaev, Yu N., A. V. Polonova, E. P. Dylenova, and V. V. Taraskin. "ISOLATION AND ANTIRADICAL ACTIVITY OF A MIXTURE OF VISNADINE AND DIHYDROSAMIDINE FROM PHLOJODICARPUS SIBIRICUS." In Современные тенденции развития технологий здоровьесбережения. Москва: Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт лекарственных и ароматических растений", 2022. http://dx.doi.org/10.52101/9785870191058_285.
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Antonijević, Marko, Jelena Đorović Jovanović, Žiko Milanović, Edina Avdović, Dejan Milenković, Dušica Simijonović, Zorica Petrović, and Zoran Marković. "Antiradical activity of N'-(1-(2,4-dioxochroman-3-yl)ethyl)-4-hydroxybenzohydrazide-thermodynamic DFT study." In The 24th International Electronic Conference on Synthetic Organic Chemistry. Basel, Switzerland: MDPI, 2020. http://dx.doi.org/10.3390/ecsoc-24-08372.
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Konrade, Daiga, and Kriss Spalvins. "Extraction of bioactives from pumpkin by-products and determination of their antioxidant activity." In Research for Rural Development 2022 : annual 28th international scientific conference proceedings. Latvia University of Life Sciences and Technologies, 2022. http://dx.doi.org/10.22616/rrd.28.2022.016.
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Agro-industrial and food processing from pumpkins (Cucurbitaceae) produces a large number of by-products: bark, pomace, seeds still rich in bioactive compounds, especially carotenoids and green pigments (proto chlorophyll (a and b) and proto pheophytin (a and b)), which exhibit a broad spectrum of health-promoting effects and can be used as ingredients in functional food and cosmetics. For extraction of bioactive compounds from dried pumpkin by-products different methods were used: supercritical CO2, Soxhlet extraction with n- hexane, ethanol. Vegetable oils (rapeseed, coconut, grapeseed and olive oil) were used as green solvent alternatives to conventional organic solvents for carotenoid extraction. Detection and analyses of chlorophylls and carotenoids was done with hexane/acetone, cyclohexane, ethanol as solvents. The aim of this study was to use pumpkin by-products for extraction of high-value bioactive compounds with different methods, to determine antioxidant´s content and profile – carotenoids (ß-carotene, lutein, lycopene, zeaxanthin), pigments (chlorophylls a, chlorophylls b) with different solvents and to find out what solvent can be used for detection of pigments and carotenoids; to determine antiradical scavenging activity of biologically active compounds in extracts from pumpkin by-products (peel and hulled seeds).
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Jansone, Liene, Solvita Kampuse, Zanda Kruma, and Ivo Lidums. "Evaluation of physical and chemical composition of concentrated fermented cabbage juice." In Research for Rural Development 2021 : annual 27th International scientific conference proceedings. Latvia University of Life Sciences and Technologies, 2021. http://dx.doi.org/10.22616/rrd.27.2021.012.
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Fermented products have gained worldwide popularity for their nutritional and health aspects. Many studies have been done on this topic, including fermented cabbage (sauerkraut). Yet little or no studies are done on evaluation of fermented cabbage juice which is considered as by-product of sauerkraut production, still rich in bioactive compounds. In order to reduce food waste, sustainable solutions are being searched for to preserve valuable fermented cabbage juice. The aim of this study was to evaluate chemical and physical composition of concentrated fermented cabbage juice and their changes after storage. The fermented cabbage juice was concentrated on falling film evaporator from 9.2 till 34.3 °Brix. Physio-chemical (moisture, pH, total soluble solids, total phenol content, antiradical activity by DPPH and ABTS+, ascorbic acid, total sugar profile, nitrates and minerals) and microbiological (lactic acid bacteria, total plate count) analyses were carried out. Concentrated fermented cabbage juice is a source of minerals and phenol compounds as well as salt substitute in food applications. After 6 months of storage there is significant degradation of ascorbic acid but total phenol content is not affected. The evaporation process did not inhibit microbiological activity; as a result, there is a decrease in lactic acid bacteria but increase in total plate count.