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Dissertations / Theses on the topic 'Antisense gene'

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Published: 4 June 2021

Last updated: 26 January 2023

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1

Aparicio, i. Prat Estel. "Natural antisense transcripts control LEF1 gene expression." Doctoral thesis, Universitat Pompeu Fabra, 2014. http://hdl.handle.net/10803/299211.

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Non-coding RNA functions are emerging in the recent years. In this thesis we describe a Natural Antisense Transcript (NAT) that controls the expression of LEF1 transcriptional factor. This LEF1 NAT is transcribed from a promoter present in the first LEF1 intron and undergoes splicing in mesenchymal cells. In epithelial cells, there is no expression of LEF1 NAT. However, in metastable epithelial cells, LEF1 NAT is transcribed and a significant part of it remains unspliced and, contrarily to the spliced NAT, down-regulates the main LEF1 promoter and LEF1 mRNA and protein expression. Moreo

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2

Aparicio, i. Prat Estel 1986. "Natural antisense transcripts control LEF1 gene expression." Doctoral thesis, Universitat Pompeu Fabra, 2014. http://hdl.handle.net/10803/299211.

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Non-coding RNA functions are emerging in the recent years. In this thesis we describe a Natural Antisense Transcript (NAT) that controls the expression of LEF1 transcriptional factor. This LEF1 NAT is transcribed from a promoter present in the first LEF1 intron and undergoes splicing in mesenchymal cells. In epithelial cells, there is no expression of LEF1 NAT. However, in metastable epithelial cells, LEF1 NAT is transcribed and a significant part of it remains unspliced and, contrarily to the spliced NAT, down-regulates the main LEF1 promoter and LEF1 mRNA and protein expression. Moreover, un

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3

Mitrpant, Chalermchai. "Pre-mRNA splicing manipulation via Antisense Oligomers." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2009. https://ro.ecu.edu.au/theses/421.

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Duchenne muscular dystrophy (DMD), the most common lethal neuromuscular disease in childhood, arises from protein-truncating mutations in the dystrophin gene. A deficiency in dystrophin leads to loss of the dystrophin associated protein complex (DAPC), which in turn, renders muscle fibres vulnerable to injury, and eventually leads to muscle loss, necrosis and fibrosis. Although, the dystrophin gene was identified nearly two decades ago, and extensive research has been directed at finding a therapy for DMD, to date, there is still no effective treatment available. One promising molecular approa

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4

Raponi, Mitch Biochemistry &amp Molecular Genetics UNSW. "Antisense RNA-mediated gene silencing in fission yeast." Awarded by:University of New South Wales. Biochemistry and Molecular Genetics, 2001. http://handle.unsw.edu.au/1959.4/18277.

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The major aims of this thesis were to investigate the influence of i) antisense gene location relative to the target gene locus (?????location effect?????), ii) double-stranded RNA (dsRNA) formation, and iii) over-expression of host-encoded proteins on antisense RNA-mediated gene regulation. To test the location effect hypothesis, strains were generated which contained the target lacZ gene at a fixed location and the antisense lacZ gene at various genomic locations including all arms of the three fission yeast chomosomes and in close proximity to the target gene locus. A long inverse-PCR proto

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5

Whitfield, Peter Cyril. "Gene expression after global and focal cerebral ischaemia." Thesis, University of Southampton, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242632.

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6

Dryselius, Rikard. "Bacterial gene expression inhibition with antisense peptide nucleic acids /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-338-8/.

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7

Watson, Colin Francis. "Altered gene expression in tomato plants transformed with polygalacturonase antisense and sense genes." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306588.

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8

Engdahl, Hilde Merete. "Natural and artificial antisense RNA : a study of inhibition of gene expression /." Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 2000. http://epsilon.slu.se/avh/2000/91-576-5784-X.pdf.

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9

Deb, Maharshi Krishna. "Generation of antisense RNAs at convergent gene loci in cells undergoing senescence." Thesis, Toulouse 3, 2016. http://www.theses.fr/2016TOU30274.

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La sénescence, qui est un mécanisme antitumoral majeur, est définie comme un état d'arrêt irréversible de la prolifération cellulaire en réponse à un stress comme l'activation illégitime d'oncogènes. Les cellules qui entrent en sénescence subissent de profonds changements de leur épigénome. Les ARNs antisens sont suspectés de joue des rôles importants dans le contrôle du destin cellulaire et dans des processus cellulaires variés. Dans la levure, le variant d'histone H2A.Z co-opère avec les machineries du RNAi et de l'hétérochromatine pour réprimer sur les loci de gènes convergents l'apparition

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10

Sivulich, Mary. "A role for antisense transcripts in regulation of viral gene expression." Connect to resource, 2008. http://hdl.handle.net/1811/32072.

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11

Faridani, Omid Reza. "Studies on natural antisense RNAs and microRNAs /." Stockholm : Karolinska institutet, 2007. http://diss.kib.ki.se/2007/978-91-7357-412-9/.

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12

Li, Jing. "Targeted degradation of RNA by RNase H using stable DNA hairpin oligomers and a study on the effect of temperature and divalent cations on RNA conformational states." Diss., Georgia Institute of Technology, 2002. http://hdl.handle.net/1853/25213.

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13

Hawley, Patricia. "Oligodeoxynucleotide interaction with mammalian cells." Thesis, University of East Anglia, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338058.

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14

Errington, Stephen J. "Target selection for antisense oligonucleotide induced exon skipping in the dystrophin gene." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2004. https://ro.ecu.edu.au/theses/794.

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Duchene Muscular Dystrophy (DMD), and the milder allelic Becker muscular dystrophy (EMD), are X-linked recessive muscle wasting disorders characterised by mutations in the dystrophin gene. DMD occurs at a frequency of approximately 1 in 3500 male newborns and life expectancy is typically less than 30 years. Due to progressive muscle wasting, affected boys are restricted to a wheelchair by the age of 12 years. The most common cause of death is pneumonia, compounded by cardiac involvement. Mutations that disrupt the dystrophin reading frame, or prevent the synthesis of either terminus, preclude

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15

Choudhury, Monideepa. "Antisense oligodeoxynucleotide-mediated alterations in slow troponin C gene expression during myogenesis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq24454.pdf.

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16

Shaharuddin, Noor A. "The process of target mRNA degradation in Antisense and sense gene silencing." Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.519393.

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17

Decani, Yol Betul. "Extension Of Flower Longevity In Transgenic Plants Via Antisense Blockage Of Ethylene Biosynthesis." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/3/12605135/index.pdf.

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Ethylene (C2H4) is a very simple molecule, a gas, and has numerous effects on the growth, development and storage life of many fruits, vegetables and ornamental crops. In higher plants, ethylene is produced from L-methionine in essentially all tissues and ACC Synthase and ACC Oxidase are the two key enzymes in the biosynthesis of ethylene. The objective of the present study was to transform tobacco (Nicotiana tabacum L. cv. Samsun) plant with partial sequence of torenia acc oxidase gene in antisense and sense orientations via Agrobacterium-mediated gene transfer system, and to analyze its effe

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18

Rush, Gavin John. "Gene expression in Mycobacteria : attenuation of gene expression by antisense methods and translation enhancement by downstream box elements." Doctoral thesis, University of Cape Town, 2004. http://hdl.handle.net/11427/2718.

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19

Day, Anthony George. "Suppression of viral and endogenous gene expression in N. tabacum using antisense RNA." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/46277.

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20

Murray, Robert John Stuart. "Investigation of a developmentally linked transcriptional gene silencing mechanism involving an antisense RNA." Thesis, University of Leicester, 2009. http://hdl.handle.net/2381/9939.

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An increasing number of RNA targeted gene repression mechanisms are being discovered operating in mammalian cells. Antisense-RNA mediated silencing of CpG island-associated genes is not limited to imprinting and X inactivation, but can occur at autosomal non-imprinted loci leading to disease. An antisense silencing phenomenon observed in a patient with an inherited form of anaemia was recently recreated in differentiating mouse embryonic stem cells, demonstrating that the silencing and methylation of the oppositely transcribed tissue specific alpha globin CpG island exclusively occurs in the p

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21

Pandey, Radha Raman. "Molecular Insights into Kcnq1ot1 Noncoding Antisense RNA Mediated Long Range Transcriptional Gene Silencing." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9392.

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22

Au, Siu Kie. "Gene expression profiling of non-small cell lung cancer using cDNA microarrays /." access full-text access abstract and table of contents, 2009. http://libweb.cityu.edu.hk/cgi-bin/ezdb/thesis.pl?phd-bch-b23749490f.pdf.

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Thesis (Ph.D.)--City University of Hong Kong, 2009.<br>"Submitted to Department of Biology and Chemistry in partial fulfillment of the requirements for the degree of Doctor of Philosophy." Includes bibliographical references (leaves 133-147)

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23

Xu, Jian, and 徐堅. "Using antisense oligonucleotide in whole embryo culture to study gene interactions during mouse gastrulation." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31220150.

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24

Xu, Jian. "Using antisense oligonucleotide in whole embryo culture to study gene interactions during mouse gastrulation /." Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19918884.

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25

Black, E. J. "Studies on the use of antisense RNA to control gene expression in Nicotiana tabacum." Thesis, Imperial College London, 1987. http://hdl.handle.net/10044/1/38236.

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26

Yang, Zhiyi. "Maize 18 kDa heat shock gene expression revealed by antisense RNA in situ hybridization." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/mq21117.pdf.

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27

Jones, Richard A. "The use of antisense-mediated gene silencing to study tissue transglutaminase in ECV304 cells." Thesis, Nottingham Trent University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369238.

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28

Turgut, Kenan. "A study of anther gene function in Brassica napus using an antisense RNA approach." Thesis, University of Leicester, 1993. http://hdl.handle.net/2381/35360.

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29

Murray, Struan Charles. "A new level of gene regulation : establishing a genome-wide role for antisense transcription." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:e8d4ce6d-f4cf-4a6c-b3c8-f6e16217214b.

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Transcription lies at the centre of gene expression. In eukaryotes, transcription occurs not only at genes but also across the non-coding portion of the genome, an apparently pervasive process that gives rise to a wide array of different transcripts. In recent years, it has emerged that genes themselves are frequently subject to non-coding transcription of their antisense strand. This antisense transcription is evident in eukaryotes from yeast to mammals; however its general genome-wide role, if indeed it has one, remains elusive. Here, the nature of antisense transcription in the budding yeas

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30

Dixon, Maria S. "ANTISENSE AFP TRANSCRIPTS IN MOUSE LIVER AND THEIR POTENTIAL ROLE IN AFP GENE REGULATION." UKnowledge, 2017. http://uknowledge.uky.edu/microbio_etds/14.

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Hepatocellular carcinoma (HCC) is the most common form of primary liver cancer, ranking the sixth most common cancer and third most common cause of cancer mortality worldwide. Alpha-fetoprotein (AFP) is a plasma protein that is highly expressed in the fetal liver and shut off after birth. AFP expression is elevated in regenerating adult liver and HCC and has been used extensively as a diagnostic marker of liver cancer. We have been studying mouse liver gene regulation to better understand mechanisms by which changes in gene expression contribute to liver development, homeostasis and disease. Z

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31

Koh, Chee Guan. "Microfluidic Assembly Of Nanoparticles For Gene/Drug Delivery." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1218638305.

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32

Almeida, Carina Marisa dos Santos. "Gold nanoparticle-DNA conjugates for oligonucleotide vectorization towards gene silencing." Master's thesis, Faculdade de Ciências e Tecnologia, 2011. http://hdl.handle.net/10362/6212.

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Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina<br>The main objective of the work presented in this thesis was to develop a gene silencing system by taking advantage of the nanovectorization capability and optical properties of gold nanoparticles. The idea is based on the construction of a DNA structure containing a therapeutic oligonucleotide with the ability to form Hoogsteen hydrogen bonds with double-stranded DNA, producing a DNA triple helix, besides silencing the gene of interest. Hoogsteen bonds, more unstable than the conventional Watson-Crick bonds, pe

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33

Velkme, Erik. "A novel method for antisense oligonucleotide gene expression manipulation in toxigenic cyanobacterial species, Microcystis aeruginosa." OpenSIUC, 2020. https://opensiuc.lib.siu.edu/theses/2781.

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Algal blooms caused by toxigenic cyanobacterial species are an increasing economic burden globally, as high anthropogenic inputs of nitrogen and phosphorous, coupled with rising levels of atmospheric CO2, promote eutrophication and enhance bloom proliferation. Of the freshwater bloom forming species, Microcystis aeruginosa has garnered the most attention due to the production of toxic secondary metabolites known as microcystins. These cyclic peptides are potent eukaryotic protein phosphatase 1 and 2A inhibitors, and can induce hepatic damage if concentration levels reach above the World Health

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34

Viscomi, Natalie Suzanne. "The role of DEAF-1 antisense transcripts in G0S2 gene expression and methylation of DNA /." Available to subscribers only, 2007. http://proquest.umi.com/pqdweb?did=1400950171&sid=3&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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35

Ehrensberger, Kate M. "Identifying modes of zinc-dependent gene regulation in S. pombe." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1388328314.

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36

Svahn, Mathias G. "DNA analogs for the purpose of gene therapy /." Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-290-3/.

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37

Carlile, Mark. "Post-transcriptional regulation of the npt2-gene locus in Zebrafish and mouse by a natural antisense transcript." Thesis, University of Newcastle upon Tyne, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506431.

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38

Rajeshwaran, Rajkumar. "EVOLUTIONARY CONSERVATION OF A POTENTIAL ANTISENSE MECHANISM FOR SAS10 AND RNP-4F GENE EXPRESSION CONTROL IN DROSOPHILA." Miami University / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=miami1154706813.

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39

Raghavan, Bindu. "Analysis of the Human Cytomegalovirus Transcriptome and Identification and Characterization of a HCMV gene involved in disruption of Interferon Signaling." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1218473086.

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40

Conley, Andrew Berton. "Alteration of transcription by non-coding elements in the human genome." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/44838.

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The human genome contains ~1.5% coding sequence, with the remaining 98.5% being non-coding. The functional potential of the majority of this non-coding sequence remains unknown. Much of this non-coding sequence is derived from transposable element (TE) sequences. These TE sequences contain their own regulatory information, e.g. promoter and transcription factor binding sites. Given the large number of these sequences, over 4 million in the human genome, it would be expected that the regulatory information that they contain would affect the expression of nearby genes. This dissertation des

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41

Thomas, Patrick Brian. "Characterisation of the long noncoding RNA GHSROS in prostate and breast cancer." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/122967/1/Patrick_Thomas_Thesis.pdf.

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In the past decade, there has been significant progress towards characterising genes in the human genome – an effort which unexpectedly revealed a large number of long noncoding RNAs with important regulatory functions in normal physiology and disease processes such as cancer. This thesis represents the first comprehensive study on the ghrelin receptor long noncoding RNA, GHSROS, in breast and prostate cancer. This work has demonstrated that GHSROS reprograms cancer cells towards a more aggressive and chemoresistant phenotype. GHSROS might represent a useful target for RNA-based therapeutics f

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42

Elmén, Joacim. "Nucleic acid based therapeutic approaches /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-047-8/.

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43

Borsu, Laetitia. "Découverte du gène AROM, "Antisense RNA Overlapping MCH gene" : caractérisation d'une nouvelle famille de protéines liant les acides nucléiques." Nice, 2000. http://www.theses.fr/2000NICE5477.

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Chez les mammifères, le gène de la MCH pour " melanin concentrating hormone", code pour deux peptides le NEI et la MCH. Ce gène est impliqué dans de nombreuses fonctions comme la réponse au stress et le contrôle de la prise alimentaire. Nous avons mis en évidence dans des cellules PC12 non stimulées l'expression d'ARN de hauts poids moléculaires contenant les séquences du gène MCH. En combinant les techniques de Northern blot, RACE PCR et RT-PCR, nous avons établi que ces ARN sont transcrits à partir des séquences du brin d'ADN complémentaire au gène de la MCH. Ce gène appelé AROM pour " antis

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44

Lloréns, Rico Verónica 1989. "Integrative understanding of transcription in a minimal cell model." Doctoral thesis, Universitat Pompeu Fabra, 2016. http://hdl.handle.net/10803/459003.

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One of the major challenges of biology is to understand how entire cells or organisms behave in homeostasis and in response to perturbations. Whole-cell modeling promotes this understanding by integrating different cellular processes in a single model that is able to predict emergent cellular behaviors. In this thesis, we have developed the first whole-cell model of the genome-reduced bacterium Mycoplasma pneumoniae, which encodes for less than 700 protein-coding genes. This model follows the structure of the previously described model in Mycoplasma genitalium. However, the lack of comprehensi

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45

Cheung, Mei-sze. "The application of an Epstein-Barr Virus specific antisense ribozyme for the in vitro suppression of EBNA-1 and LMP-1 expression." Click to view the E-thesis via HKUTO, 2002. http://sunzi.lib.hku.hk/hkuto/record/B42577056.

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46

Galante, Pedro Alexandre Favoretto. "Uso de técnicas computacionais no estudo da transcrição e regulação gênica em Homo sapiens e Mus musculus." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-28032008-092635/.

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O gene, uma seqüência de nucleotídeos necessária para a síntese de moléculas funcionais, é transcrito e regulado por um conjunto de processos e fatores extremamente complexos. Entender o momento e o tecido em que os genes são expressos, as isoformas funcionais, as regiões controladoras e os fatores envolvidos na regulação da expressão de cada gene é um dos grandes desafios da biologia molecular moderna. Hoje, com a enorme quantidade de informações de seqüências genômicas e de transcriptomas, aliado ao desenvolvimento de métodos computacionais para agrupar e analisar estes dados em larga

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47

Chessher, Ashley John. "The application of antisense silencing for the characterisation of essential gene stringency and for the development of species-specific antimicrobials." Thesis, University of Newcastle upon Tyne, 2014. http://hdl.handle.net/10443/2362.

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The emergence of multiple-antibiotic resistance among clinical pathogens has created an urgent requirement for the development of new antibiotics. The current lack of new antibiotics has not only renewed interest in traditional natural product screening approaches, but also prompted efforts to develop alternate antimicrobial strategies. Antisense RNA based silencing provides a strategy for developing whole cell screening assays, whereby antisense RNA induction leads to target protein depletion and subsequently the increased sensitivity of test organisms to target specific inhibitors. The devel

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48

Anson, Simon Richard. "An investigation into the molecular role of qrf, a natural antisense transcript of the Neurospora crassa circadian clock gene frequency." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.702310.

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49

Cheung, Mei-sze, and 張美思. "The application of an Epstein-Barr Virus specific antisense ribozyme for the in vitro suppression of EBNA-1 and LMP-1 expression." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B42577056.

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50

Thakur, Noopur. "Long-range Control of Gene Expression by Imprinting Control Regions During Development and Neoplasia." Doctoral thesis, Uppsala universitet, Zoologisk utvecklingsbiologi, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5725.

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Genomic imprinting is an epigenetic phenomenon by which a subset of genes is expressed in a parent of origin specific manner. Most of the imprinted genes are located in clusters. Genetic evidences suggest that genes in imprinted clusters are regulated by Imprinting Control Regions (ICRs). To elucidate the mechanisms by which the imprinting is maintained in clusters, we have chosen a well characterized cluster at the distal end of mouse chromosome 7. This cluster contains 15 imprinted genes and they have been shown to be regulated by H19 and Kcnq1 ICRs. The mouse H19 ICR, which is shown to have

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