To see the other types of publications on this topic, follow the link: API 20E.

Journal articles on the topic 'API 20E'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'API 20E.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Topic Popovic, N., R. Coz-Rakovac, and I. Strunjak-Perovic. "Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review." Veterinární Medicína 52, No. 2 (2008): 49–53. http://dx.doi.org/10.17221/2058-vetmed.

Full text
Abstract:
The available data concerning rapid identification of fish bacteria via commercial phenotypic tests demonstrate that there is no agreement regarding the choice of the tests. However, API 20E, an identification system for Enterobacteriaceae and other non-fastidious Gram-negative rods developed for clinical specimens, seems to be increasingly used for the identification of fish pathogens. In this review, adaptation of API 20E for fish bacterial isolates and its distinctiveness for fish bacteria was assessed. Some strains are wrongly identified because they are not included in the database of API 20E system. API 20E reactions should be compared with the diagnostic schemes based on reactions in conventional phenotypic tests. Due to their significance for fish health and impact on the aquaculture, and because of the need for their rapid identification, some important fish bacteria should be included in the API 20E system, such as <i>Yersinia ruckeri</i>, <i>Edwardsiella ictaluri</i>, <i>Vibrio anguillarum</i>.
APA, Harvard, Vancouver, ISO, and other styles
2

Patyka, V., L. Butsenko, and L. Pasichnyk. "Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria." Agricultural Science and Practice 3, no. 1 (2016): 43–48. http://dx.doi.org/10.15407/agrisp3.01.043.

Full text
Abstract:
Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.
APA, Harvard, Vancouver, ISO, and other styles
3

Rakotovao-Ravahatra, Zafindrasoa Domoina, Lalaina Rahajamanana, Lalaina Rakotondraoelina, et al. "Comparison of Bis NEG-D and API 20E for the Identification of Gram-negative Bacilli in the Laboratory of the University Hospital of Befelatanana Antananarivo Madagascar." European Journal of Biology and Biotechnology 2, no. 5 (2021): 76–80. http://dx.doi.org/10.24018/ejbio.2021.2.5.286.

Full text
Abstract:
In medical analysis laboratories, techniques for identifying bacteria are currently becoming more and more numerous. The objective of this study is to compare the 2 bacterial identification systems Bis NEG-D and Api 20E for the identification of gram-negative bacilli. This is a qualitative evaluation of the Bis NEG-D compared to the gold standard Api 20E. During the study period, 32 Gram-negative bacilli isolates were identified simultaneously using Api 20E and Bis NEG-D. The samples are represented by 12 (37.5%) blood samples for blood culture, 12 (37.5%) urine samples for cytobacteriological examination of the urine, 06 (18.8%) pus samples for bacteriological examination of pus, a sample of the cerebrospinal fluid (3.1%) and a vaginal sample (3.1%).The bacteria identified were represented by Enterobacter spp, Escherichia col,i Klebsiella pneumonia, Shigella spp, Salmonella typhi, Acinetobacter baumannii, Pseudomonas spp, Proteus mirabilis, Raoultella ornithinolytica and Bukholderia cepacia. This study showed a concordance of 90.6% (29/32) and a discordance of 9.4% (3/32) between the results of Api 20E and Bis NEG-D. Concerning the probability scores, they vary between 95% to 100% for Api 20E and between 79.3% to 100% for Bis NEG-D. This study also compared the pros and cons of using Api 20 E and Bis NEG-D. The Bis-NEG-D is valid and can be used by medical analysis laboratories like the Api 20E, especially if these laboratories do not need to perform a lot of bacterial identification tests.
APA, Harvard, Vancouver, ISO, and other styles
4

Rennie, R. J. "The API 20B microtube system to aid in the identification of N2-fixing Bacillaceae." Canadian Journal of Microbiology 33, no. 6 (1987): 504–9. http://dx.doi.org/10.1139/m87-084.

Full text
Abstract:
N2-fixing bacterial isolates from soil have been identified successfully using the API 20E and 50E microtubes in conjunction with a computer-assisted biochemical profile search. The 20E and 50E result in few positive tests with N2-fixing Bacillaceae. Recently, a new battery of 20 microtube tests, the 20B, has been introduced for identifying aerobic, heterotrophic bacteria isolated from natural environments. This system was compared with the 20E and evaluated for N2-fixing bacillus species from soil. It was then used in conjunction with guanine-plus-cytosine analysis to identify the C-11-25 (5DN+) bacillus strain that fixes N2 in association with Canadian wheat cultivars. The API 20E and 20B gave similar results for many biochemical tests, but the 20E resulted in negative tests for carbohydrate utilization by bacillus strains. The use of different growth media or 0.85% NaCl to suspend these bacteria prior to inoculating the cupules did not alter the inability of the 20E in this manner. Carbohydrate utilization in the 20B system agreed well with that in the traditional utilization tests. Hence, I concluded that the API 20E is not suitable to evaluate carbohydrate utilization in N2-fixing Bacillaceae. The spore-forming, acetylene-reducing C-11-25 strain of bacillus was biochemically similar to the ATCC type culture of Bacillus polymyxa (strain 842). Its guanine-plus-cytosine ratio of 48.0 mol% was similar to that of B. polymyxa (43–46 mol%). A general procedure for the isolation and identification of N2-fixing Bacillus from soil is proposed.
APA, Harvard, Vancouver, ISO, and other styles
5

Neubauer, Heinrich, Thomas Sauer, Heinz Becker, Stojanca Aleksic, and Hermann Meyer. "Comparison of Systems for Identification and Differentiation of Species within the GenusYersinia." Journal of Clinical Microbiology 36, no. 11 (1998): 3366–68. http://dx.doi.org/10.1128/jcm.36.11.3366-3368.1998.

Full text
Abstract:
Of four tested identification systems (API 20E, API Rapid 32 IDE, Micronaut E, and the PCR-based Yersinia enterocoliticaAmplification Set), API 20E is still the system of choice for identifying pathogenic Yersinia isolates. It provides the highest sensitivity both at the genus and at the species level and has the best cost-effectiveness correlation.
APA, Harvard, Vancouver, ISO, and other styles
6

Artati, Diah, and Moh Oman. "IDENTIFIKASI BAKTERI Aeromonas hydrophila MENGGUNAKAN KIT API 20 E DI LABORATORIUM MIKROBIOLOGI BRPI SUKAMANDI." Buletin Teknik Litkayasa Akuakultur 18, no. 1 (2020): 75. http://dx.doi.org/10.15578/blta.18.1.2020.75-80.

Full text
Abstract:
Metode identifikasi bakteri dapat dilakukan berdasarkan morfologi sel, uji aktivitas biokimia, analisis DNA, dan uji serologis. Identifikasi bakteri melalui pengamatan aktivitas biokimia saat ini bisa lebih mudah dengan menggunakan kit API (Analytical Profile Index). API 20E berguna untuk mengidentifikasi spesies dan subspesies Enterobacteriaceae dan identifikasi kelompok serta spesies mikroorganisme non fermentatif. Kegiatan identifikasi bakteri Aeromonas hydrophila dengan sampel ATCC 35654 menggunakan kit API 20E dilakukan di Laboratorium Mikrobiologi Balai Riset Pemuliaan Ikan, Sukamandi dengan tujuan untuk mengetahui kemampuan KIT API 20 E dalam mengidentifikasi ATCC 35654 yang akan digunakan sebagai jaminan mutu pengujian dalam kegiatan identifikasi bakteri Aeromonas hydrophila. Tahap-tahap pengujian yang dikerjakan meliputi uji oksidase, preparasi strip kit API 20E, preparasi inokulum dan proses inokulasi pada strip, pembacaan strip, penambahan reagen, dan proses pengisian data menggunakan Software. Kit API 20 E mampu mengidentifikasi ATCC 35654 sebagai bakteri Aeromonas hydrophila dengan %ID sebesar 91,5 dan ATCC 35654 dapat digunakan sebagai jaminan mutu pengujian dalam kegiatan identifikasi Aeromonas hydrophila.
APA, Harvard, Vancouver, ISO, and other styles
7

Atanasova, Iliana, Petia Kabadjova, Nevena Bogatzevska, and Penka Moncheva. "New Host Plants of Erwinia amylovora in Bulgaria." Zeitschrift für Naturforschung C 60, no. 11-12 (2005): 893–98. http://dx.doi.org/10.1515/znc-2005-11-1212.

Full text
Abstract:
Nine strains of Erwinia amylovora were isolated from new host plants in Bulgaria D chokeberry and strawberry. The strains were characterized morphologically and biochemically using the API 20E and BIOLOG system. It was established that they showed three different API 20E metabolic profiles, not found by previous studies of E. amylovora. All strains were identified as E. amylovora due to their metabolic fingerprint patterns obtained by the BIOLOG system. The identification was confirmed by PCR amplification of a specific region of plasmid pEA29 and genome ams-region. This study is the first characterization and identification of E. amylovora strains isolated from chokeberry and strawberry by the API 20E and BIOLOG system and by polymerase chain reaction.
APA, Harvard, Vancouver, ISO, and other styles
8

Sharma, N. K., P. W. Doyle, S. A. Gerbasi, and J. H. Jessop. "Identification of Yersinia species by the API 20E." Journal of Clinical Microbiology 28, no. 6 (1990): 1443–44. http://dx.doi.org/10.1128/jcm.28.6.1443-1444.1990.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Chang, Miwha, Haeyong Lee, Joo-Young Kim, Won-Hae Lee, Chong Min Choung та Seohyun Moon. "부검체 유래의 Escherichia coli와 Shigella spp. 동정을 위한 16S rRNA 유전자 염기서열, API 20E 및 RT-PCR 분석법의 비교". Korean Academy of Scientific Criminal Investigation 16, № 3 (2022): 163–77. http://dx.doi.org/10.20297/jsci.2022.16.3.163.

Full text
Abstract:
Practical techniques to differentiate Escherichia coli from Shigella spp. remains underexplored in the field of forensic microbiology. We aimed to compare the performance of 16S rRNA gene sequencing, and two commercially available API 20E and RT-PCR systems to distinguish between E. coli and Shigella spp. on the basis of analysis of 24 isolates collected from 12 forensic autopsies to evaluate the level of species identification. The accuracy of 16S rRNA gene sequencing at the species level was ranged from 16.7% to 33.3%. However, 79.2% (19/24) were unidentified, even though analyzed using three distinct databases (MicroSEQ 500 library, BLAST, EzTaxon). Phylogenetic analysis confirmed that 16S rRNA gene sequencing could not differentiate Shigella spp. and E. coli. Of the 19 isolates, the API 20E showed complete identification of 78.9% (15/19) at the species level, whereas RT-PCR analysis showed complete identification of 19 isolates (100%) at the species level. Our comparison analyses revealed that only 16S rRNA gene sequencing is not enough to differentiate E. coli and Shigella spp., and the accuracy and rapidity order was as follows: RT-PCR >API 20E>standard analysis. These results suggest that the polyphasic strategy with the standard analysis and RT-PCR or API 20E techniques are ideal for differentiation of these microbes.
APA, Harvard, Vancouver, ISO, and other styles
10

Artati, Diah, Mohammad Oman, Supriyanto Supriyanto, Dede Sukarta, and Adam Robisalmi. "Validation of Analytical Method for Aeromonas hydrophila Identification using Analytical Profile Index (API) 20E KIT Method." Jurnal Medik Veteriner 7, no. 1 (2024): 88–104. http://dx.doi.org/10.20473/jmv.vol7.iss1.2024.88-104.

Full text
Abstract:
The high demand for fish consumption has an impact on increasing aquaculture productivity and causes the vulnerability of increasing bacterial populations in aquaculture fields, so more rapid handling is needed. The use of phenotypic KIT methods (API 20E) has been applied as a targeted and efficient identification support in targeting better bacterial identification accuracy but often provides unequal results. Method validation is one of the general requirements for the competence of a laboratory evaluation: to provide coherent, interpretable, and accurate results with known uncertainties. The purpose of this study was to validate the API 20E KIT method for the identification of A. hydrophila. The conventional method used as a reference is SNI 7303.1:2015. The validation parameters consisted of determining the limit of detection, sensitivity, and specificity tests, as well as the positive predictive value and negative predictive value. The results showed that the limit of the detection value of the API 20E KIT was at a concentration of 100 CFU/mL with an ID of 99.00%. The sensitivity and specificity values in the positive and negative target samples were 100% with a positive predictive value and a negative predictive value of 0%, respectively. In conclusion, the API 20E KIT method as an alternative test method or rapid test was proven valid for identifying A. hydrophila by the test results using the reference method.
APA, Harvard, Vancouver, ISO, and other styles
11

Lacroix, M., L. Vézina, S. Desjardins, and C. Beaulieu. "Comparaison de techniques d’identification des Erwinia et des Pseudomonas responsables de la pourriture molle." Phytoprotection 76, no. 1 (2005): 27–37. http://dx.doi.org/10.7202/706082ar.

Full text
Abstract:
Trois méthodes, soit la caractérisation physiologique, l'utilisation de systèmes miniaturisés d'identification (API 20E, API NFT et Biolog) et l'analyse du profil électrophorétique des protéines sécrétées, ont été expérimentées afin de déterminer une technique précise et rapide d'identification des Pseudomonas et des Erwinia responsables de la pourriture molle. L'analyse des patrons électrophorétiques des protéines sécrétées est une méthode très efficace pour identifier les différentes espèces pectinolytiques de Pseudomonas fluorescents. Le système Biolog reconnaît efficacement le P. marginalis et le P. viridiflava. Le système API NFT est efficace pour l'identification du P. marginalis, du P. viridiflava et du P. syringae. C'est le système API 20E qui s'est avéré le plus efficace pour l'identification des Erwinia. L'électrophorèse des protéines sécrétées et le système API NFT permettent une identification rapide et efficace des Pseudomonas, tandis que pour les Erwinia, seul le système API20E est performant.
APA, Harvard, Vancouver, ISO, and other styles
12

MOHAMMAD, Anwar O., Abdulkareem E. S. ALKURTANY, and Abdullah A. HASSAN. "Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soil." Notulae Scientia Biologicae 12, no. 2 (2020): 258–63. http://dx.doi.org/10.15835/nsb12210628.

Full text
Abstract:
Bacterial activity, mainly Pseudomonas spp. plays a vital role in the fruiting process of white button mushroom, hence a rapid procedure to identify these bacteria is crucial. In the current study, the validity of commercial identification system, Analytical profile index API 20E to identify Pseudomonas isolates from mushroom casing soil were assessed. Using API strips fifty bacterial isolates from a selective medium (King B medium) were examined, all isolates were belonged to the genus Pseudomonas according to API 20E identification systems. However, only 74% of Pseudomonas bacteria were identified to species level. The molecular identification using 16S rRNA gene was used as a reference tool to identify bacteria at the species level. The results show that the accuracy of the system to classify florescent Pseudomonas to species level was 60%. This was species dependant, and the system accuracy were 100%, 87.5%, 81.3% and 63% in identifying P. aeruginosa, P. putida, P. fluorescens and P. tolaasii respectively. Our finding indicates that although the classification of the Pseudomonas genus with API 20E system is useful, but it is not enough to distinguish these bacteria to species level, genomic studies are necessary to confirm the exact taxonomic position of Pseudomonas spp.
APA, Harvard, Vancouver, ISO, and other styles
13

Archer, J. R., R. F. Schell, D. R. Pennell, and P. D. Wick. "Identification of Yersinia spp. with the API 20E system." Journal of Clinical Microbiology 25, no. 12 (1987): 2398–99. http://dx.doi.org/10.1128/jcm.25.12.2398-2399.1987.

Full text
APA, Harvard, Vancouver, ISO, and other styles
14

Holmes, B., and C. A. Dawson. "Misuse and interlaboratory test reproducibility of API 20E system." Journal of Clinical Pathology 38, no. 8 (1985): 937–41. http://dx.doi.org/10.1136/jcp.38.8.937.

Full text
APA, Harvard, Vancouver, ISO, and other styles
15

Simoons-Smit, A. M., A. M. J. J. Verweij-van Vught, I. Y. R. Kanis, and D. M. Maclaren. "Biochemical and serological investigations on clinical isolates of klebsiella." Journal of Hygiene 95, no. 2 (1985): 265–76. http://dx.doi.org/10.1017/s0022172400062690.

Full text
Abstract:
SUMMARYA series of 925 clinical isolates of klebsiella was examined by serological and biochemical typing. To perform serological typing (capsular swelling) 77 capsular antisera were prepared, tested against the type strains and grouped in 13 pools. With this serotyping method 80% of the cultures were typable and 63 distinct types could be recognized.All strains were typable biochemically by means of the numerical coding system of the API-20E system supplemented by digits derived from 15 additional conventional biochemical tests. With the API-20E system 24 different biotypes could be distinguished whereas the combination of API-20E and the 15 additional tests produced 93 biotypes. Maximum discrimination of strains was achieved by the combination of serological and biochemical typing (256 bioserotypes). The reproducibility, typability and discriminating power of the biotyping system was not inferior to serotyping. For epidemiological purposes biotyping can replace serotyping ofKlebsiellaspecies, especially in laboratories less well equipped.
APA, Harvard, Vancouver, ISO, and other styles
16

Overman, T. L., J. F. Kessler, and J. P. Seabolt. "Comparison of API 20E, API rapid E, and API rapid NFT for identification of members of the family Vibrionaceae." Journal of Clinical Microbiology 22, no. 5 (1985): 778–81. http://dx.doi.org/10.1128/jcm.22.5.778-781.1985.

Full text
APA, Harvard, Vancouver, ISO, and other styles
17

Atanasova, Iliana, Penka Moncheva, Petia Kabadjova, et al. "Phenotypic Diversity of Erwinia amylovora in Bulgaria." Zeitschrift für Naturforschung C 62, no. 11-12 (2007): 857–68. http://dx.doi.org/10.1515/znc-2007-11-1214.

Full text
Abstract:
Fifty-one strains of Erwinia amylovora isolated from nine host plants in Bulgaria were characterized phenotypically and identified by the API 20E and BIOLOG system. The identification was confirmed by PCR amplification of a specific region of the plasmid pEA29 and the genome ams region. The phenotypic diversity of the strains was studied on the basis of their API 20E and BIOLOG metabolic profiles, as well as of their SDS-PAGE protein profile. Metabolic diversity among the strains was established, but no connection with the origin of the strains was revealed. The Bulgarian strains showed API 20E metabolic profiles not found in previous studies of E. amylovora. The strains formed a homogenous group on the basis of their protein profiles. All the strains were sensitive to the antibiotics streptomycin, tetracycline and oxytetracycline. This study was an initial step towards an investigation of the diversity and evolution in the Bulgarian population of E. amylovora, and it was the first characterization of E. amylovora strains isolated from different host plants in the period 1995-2005 in Bulgaria.
APA, Harvard, Vancouver, ISO, and other styles
18

Amarantini, Charis, Tri Yahya Budiarso, and Tri Wahyuni Sekar Sari -. "COLIFORM CONTAMINATION LEVEL OF WASHING WATER AT STREET VENDORS TENT STALLS IN YOGYAKARTA." BIOMA : Jurnal Ilmiah Biologi 13, no. 2 (2024): 1–12. https://doi.org/10.26877/bioma.v13i2.365.

Full text
Abstract:
Food quality in Indonesia is significantly influenced by street vendors, particularly from a microbiological aspect. The use of unclean washing water in these settings can cause tableware contamination, increasing the risk of food-borne diseases. Therefore, this research aimed to determine the level of coliform contamination in washing water of street vendors. A total of 21 samples were collected from different locations, isolated, and identified morphologically and biochemically. These samples were resuscitated in 1% peptone medium for 12 hours and inoculated on Chromocult Coliform Agar (CCA) medium. Purified coliform colonies were tested morphologically and biochemically to the genus level and confirmed using API 20E KIT reagents. The results showed coliform contamination levels ranging from 5.1 x 105 to 2.7 x 108 CFU/mL, exceeding the quality standard. Confirmation results from testing using API 20E with ID ≥ 95% found bacteria contaminants, including Escherichia coli, Klebsiella pneumoniae subsp. Pneumoniae and Pantoea spp. These results showed the urgent need to recognize the risks associated with tableware contamination at tent stalls. Keywords: API 20E, Coliform, Street Vendors, Washing Water.
APA, Harvard, Vancouver, ISO, and other styles
19

Ezeamagu, Christopher E., Mande Garuba, and Toyosi F. Osisami. "Identification of Vibrio Cholerae using Analytical Profile Index (API 20E)." International Journal of Research and Innovation in Applied Science IX, no. V (2024): 108–11. http://dx.doi.org/10.51584/ijrias.2024.905009.

Full text
Abstract:
Cholera outbreak have resulted in the deaths of millions of people globally. The cholera-causing Vibrio cholerae, has multiple serogroups, and produces epidemics when two strains, O1 and O139, are present. With the exception of recent, rare instances, V. cholerae O139 caused prior outbreaks; however, V. cholerae O1 was responsible for all following epidemics. Hence, identification of the V. cholerae is an important step in the control of cholera outbreak especially in the local community where inadequate facilities for prognosis limit prompt treatment of patients. A total of 15 isolates previously identified with molecular method were subjected to TCBS Agar and Analytical Profile Index. All the sucrose-fermenting colonies on TCBS agar were correctly identified with API kit. The study concluded that API kit can be substituted for molecular method in prognosis of Cholera outbreak especially in resource-limited Countries due cost implication.
APA, Harvard, Vancouver, ISO, and other styles
20

COX, N. A., M. VAN WART, J. S. BAILEY, and J. E. THOMSON. "Identification of Enterobacteriaceae from Foods with the Spectrum-10." Journal of Food Protection 48, no. 1 (1985): 76–79. http://dx.doi.org/10.4315/0362-028x-48.1.76.

Full text
Abstract:
Spectrum-10, a newly developed miniaturized identification system, was analyzed for its ability to accurately and rapidly identify members of the Enterobacteriaceae family. This study, conducted at two separate laboratories, tested freshly isolated organisms from raw and frozen foods (180) and stock cultures (144). For comparison purposes, the Micro-ID and API-20E identification systems were concurrently inoculated with the test organisms. In comparison to the Micro-ID and the API-20E systems, the Spectrum-10 identified 95 to 96% of the stock cultures to genus and species, whereas 93% of the fresh isolates were identified to genus and 82% to species.
APA, Harvard, Vancouver, ISO, and other styles
21

Appelbaum, P. C., M. R. Jacobs, M. K. Buick, M. M. Flanagan, and G. A. Gymer. "Evaluation of the Micro-ID, the API 20E and the Rapid 20E for same-day identification ofEnterobacteriaceae." European Journal of Clinical Microbiology 4, no. 5 (1985): 498–501. http://dx.doi.org/10.1007/bf02014432.

Full text
APA, Harvard, Vancouver, ISO, and other styles
22

AONO, EIJI, HARUO SUGITA, JUNJIRO KAWASAKI, et al. "Evaluation of the Polymerase Chain Reaction Method for Identification of Vibrio vulnificus Isolated from Marine Environments." Journal of Food Protection 60, no. 1 (1997): 81–83. http://dx.doi.org/10.4315/0362-028x-60.1.81.

Full text
Abstract:
The polymerase chain reaction (PCR) method for identification of Vibrio vulnificus in the marine environment was evaluated by comparing it to both the conventional and DNA-DNA hybridization methods. Of 13,325 isolates obtained from seawater and sediment samples, and oyster and goby specimens collected from the coastal waters of Tokyo Bay, Japan, only 61 isolates were identified as V. vulnificus on the basis of phenotypic characteristics and the amplification of the cytotoxin-hemolysin gene by the PCR method. All 61 isolates were further confirmed to be V.vulnificus by a DNA-DNA hybridization method and the API 20E system although they were divided into 13 groups on the basis of their API 20E profiles. These results strongly suggest that the PCR method is useful for identification of this organism.
APA, Harvard, Vancouver, ISO, and other styles
23

Martinez-Urtaza, Jaime, Antonio Lozano-Leon, Alejandro Viña-Feas, Jacobo Novoa, and Oscar Garcia-Martin. "Differences in the API 20E biochemical patterns of clinical and environmentalVibrio parahaemolyticusisolates." FEMS Microbiology Letters 255, no. 1 (2006): 75–81. http://dx.doi.org/10.1111/j.1574-6968.2005.00052.x.

Full text
APA, Harvard, Vancouver, ISO, and other styles
24

Edinger, R. C., P. C. Migneault, and F. S. Nolte. "Supplementary rapid biochemical test panel for the API 20E bacterial identification system." Journal of Clinical Microbiology 22, no. 6 (1985): 1063–65. http://dx.doi.org/10.1128/jcm.22.6.1063-1065.1985.

Full text
APA, Harvard, Vancouver, ISO, and other styles
25

Barr, J. G., G. M. Hogg, E. T. Smyth, and A. M. Emmerson. "Comparison of identification of Enterobacteriaceae by API 20E and Sensititre Autoidentification System." Journal of Clinical Pathology 42, no. 6 (1989): 649–52. http://dx.doi.org/10.1136/jcp.42.6.649.

Full text
APA, Harvard, Vancouver, ISO, and other styles
26

Santos, Y., J. L. Romalde, I. Bandín, et al. "Usefulness of the API-20E system for the identification of bacterial fish pathogens." Aquaculture 116, no. 2-3 (1993): 111–20. http://dx.doi.org/10.1016/0044-8486(93)90002-g.

Full text
APA, Harvard, Vancouver, ISO, and other styles
27

Chao, Kuo-Kuang, Chen-Ching Chao, and Wei-Liang Chao. "Evaluation of Colilert-18 for Detection of Coliforms and Eschericha coli in Subtropical Freshwater." Applied and Environmental Microbiology 70, no. 2 (2004): 1242–44. http://dx.doi.org/10.1128/aem.70.2.1242-1244.2004.

Full text
Abstract:
ABSTRACT The accuracy of Colilert-18 as a test for coliforms and Escherichia coli in subtropical freshwater was evaluated by using API 20E strips and fatty acid methyl ester analysis. The false-positive and -negative rates of detection were 7.4 and 3.5%, respectively, for E. coli and 9.6 and 6.3%, respectively, for coliforms.
APA, Harvard, Vancouver, ISO, and other styles
28

Kattar, M. M., J. F. Chavez, A. P. Limaye, et al. "Application of 16S rRNA Gene Sequencing To IdentifyBordetella hinzii as the Causative Agent of Fatal Septicemia." Journal of Clinical Microbiology 38, no. 2 (2000): 789–94. http://dx.doi.org/10.1128/jcm.38.2.789-794.2000.

Full text
Abstract:
We report on the first case of fatal septicemia caused byBordetella hinzii. The causative organism exhibited a biochemical profile identical to that of Bordetella aviumwith three commercial identification systems (API 20E, API 20 NE, and Vitek GNI+ card). However, its cellular fatty acid profile was not typical for either B. avium or previously reported strains of B. hinzii. Presumptive identification of the patient's isolate was accomplished by traditional biochemical testing, and definitive identification was achieved by 16S rRNA gene sequence analysis. Phenotypic features useful in distinguishing B. hinzii from B. avium were production of alkali from malonate and resistance to several antimicrobial agents.
APA, Harvard, Vancouver, ISO, and other styles
29

Hero A. Omar Al-Shaik bzainy, Najat A. Zaman, and Pary L. Mohammed. "Detection of Salmonella, Shigella and Candida spp. in stool from diarrheal children and evaluation the heating effect on Salmonella phage in Kirkuk city." Tikrit Journal of Pure Science 26, no. 4 (2021): 6–11. http://dx.doi.org/10.25130/tjps.v26i4.155.

Full text
Abstract:
Background: Salmonella spp. and Shigella are two pathogenic members within the Enterobacteriaceae family, and they are causing food poisoning and diarrhea that transmits via oral route by contaminated food and water especially in children from ages 1 day to 14 years.
 Objective: focus on the study of Salmonella spp., Shigella and Candida spp. isolated from diarrheal children in Kirkuk city and diagnose via in vitro bacterial diagnosis with traditional fermentation chemical tests, API 20E, RapIDTM ONE technology and Vitek 2 compact system, as well as study the thermal stability of isolated Salmonella phage,
 Method: Collecting (200) cases of diarrhea from children in Kirkuk hospitals, through an epidemiological statistical study and conventional methods for diagnosis enteric bacteria with API 20E and RapIDTM ONE, and non-typhi Salmonella isolates (S.typhimurium) were identified with Vitek 2 compact System. Bacterial sensitivity tests to antibiotics were verified by Kerby - Bauer disk diffusion method and Candida spp. with CHROM agar Candida and API 20C AUX ; The Salmonella phage was isolated by spot assay, then exposed to different temperatures before the observation of degradation range of the exposed phage to the salmonella non-typhi, on Tryptic Soy Agar medium (TSA).
 Results: Salmonella spp., Shigella and E. coli isolated in rate of 6%, 0.5% and 51.5% respectively while intestinal candida detected in rate of 89.5% in total of 200 children were diagnosed, in which 113 samples of males (56.5%) and 87 samples of females (43.5%) (p> 0.05), the degradation range was observed to the exposed phage to degrees (35, 40) C. for (15, 30) minutes, and stopped at the exposed Salmonella phages to temperatures (45) at 30 minutes.
 Conclusion: The prevalence of salmonella and shigella was relatively low and the highest incidence of Salmonella was within the age groups ranging between (11-14) and (1-6) year compared to other age groups. Isolated strains showed multidrug resistance (MDR), As well the genus Candida albicans was the most common type compared to other intestinal Candida species in children with diarrhea. and it has been found that salmonella phage can be isolated from sewage water and chicken's droppings by simple methods.
APA, Harvard, Vancouver, ISO, and other styles
30

COX, N. A., and J. S. BAILEY. "Enterobacteriaceae Identification from Stock Cultures and High Moisture Foods with a Four-Hour System (API Rapid E)." Journal of Food Protection 49, no. 8 (1986): 605–7. http://dx.doi.org/10.4315/0362-028x-49.8.605.

Full text
Abstract:
The API Rapid E is a 4-h system for the identification of Enterobacteriaceae that has not previously been evaluated with food isolates. A total of 232 cultures, representing 13 genera of Enterobacteriaceae, was used in this study; 47 were known stock cultures and 185 were freshly isolated from raw foods (broiler carcasses, chicken sausage, hamburger, scallops and shrimp). Each food isolate was inoculated into the API Rapid E and also into two other miniaturized systems (Micro-ID and API-20E) which served as the reference. API Rapid E correctly identified 219 (94.4%) of the cultures to species. Ten of the thirteen errors in identification occurred with Enterobacter spp. because of false-negative reactions with the Voges-Proskauer test. The predominant Enterobacteriaceae encountered in each food were Escherichia coli (broiler carcasses), Serratia marcescens (chicken sausage), Enterobacter aerogenes (hamburger), Enterobacter cloacae (scallops) and Klebsiella oxytoca (shrimp). The degree of accuracy with the variety of organisms tested in this study coupled with the 4-h incubation should make the API Rapid E a practical alternative to conventional procedures for the practicing food microbiologist.
APA, Harvard, Vancouver, ISO, and other styles
31

Tunca, Rahsan Ivgin, Ozgur Ceylan, and Okan Ozgul. "Determination of intestinal microbiome of Marchalina hellenica (Gennadius, 1883) (Hemiptera, Margarodidae)." Boletín de la Asociación española de Entomología 48, no. 1-2 (2024): 11–20. http://dx.doi.org/10.70186/baeehfgr3179.

Full text
Abstract:
Knowledge of the insect microbiota remains largely unexplored. The intestinal microbiome of Marchalina hellenica (Gennadius, 1883) (Hemiptera, Margarodidae) was determined in the current study. This species is the main source of pine honey production. A pool of 100 samples were selected, representing the distribution of M. hellenica in the province of Mugla, Turkey. API Staph tests were used to assess Gram-positive bacteria, while API 20NE and API 20E tests were used to assess Gram-negative bacteria. As a result, 33 Gram-negative and 15 Gram-positive isolates were obtained from samples collected from 44 different locations. Among Gram-positive, 14 out of 15 belonged to Staphylococcus spp. Among Gram-negative, 22 out of 33 belonged to Aeromonas spp. This is the first study intended to determine the intestinal microbiome of M. hellenica.
APA, Harvard, Vancouver, ISO, and other styles
32

Holmes, B., and P. S. Humphry. "Four hour identification of Enterobacteriaceae with the API Rapid 20E and Micro-ID systems." Journal of Clinical Pathology 38, no. 10 (1985): 1132–38. http://dx.doi.org/10.1136/jcp.38.10.1132.

Full text
APA, Harvard, Vancouver, ISO, and other styles
33

GRISEZ, L., R. CEUSTERS, and F. OLLEVIER. "The use of API 20E for the identification of Vibrio anguillarum and V. ordalii." Journal of Fish Diseases 14, no. 3 (1991): 359–65. http://dx.doi.org/10.1111/j.1365-2761.1991.tb00833.x.

Full text
APA, Harvard, Vancouver, ISO, and other styles
34

Mancini, Maria Emanuela, Matteo Beverelli, Adelia Donatiello, et al. "Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods." PLOS ONE 17, no. 7 (2022): e0268706. http://dx.doi.org/10.1371/journal.pone.0268706.

Full text
Abstract:
Yersiniosis is the third most reported food-borne zoonosis in Europe. The aim of the present study was to perform the search for Yersinia enterocolitica in food samples collected from Apulia and Basilicata regions (Southern Italy) and to characterize any isolates by classical and modern analytical methods. A total of 130 samples were analyzed between July 2018 and July 2019: most of them were raw milk and dairy products made from it. Furthermore, 8 out of 130 samples were individual milk samples collected from bovines reared in a Brucella-free farm which showed false positive serological reaction for brucellosis due to the presence of pathogenic Y. enterocolitica O:9 biotype 2 in faeces. The Real Time PCR targeting the ail gene and the culture method were performed to detect pathogenic Y. enterocolitica. Isolates were subjected to API 20E (Biomerieux) and MALDI-TOF MS (Matrix Assisted Laser Desorption Ionization Time-of-Flight) for species identification. All samples were negative for the ail gene. The culture method allowed to isolate suspicious colonies from 28 samples. The API 20E system and the MALDI-TOF MS technique identified 20 Y. enterocolitica and 1 Y. intermedia in a concordant way. The remaining 7 strains were all identified as Y. enterocolitica by the API 20E system, while the MALDI-TOF MS recognized 4 Y. intermedia, 1 Y. bercovieri and 2 Y. massiliensis. Genotypic characterization of the discordant strains was performed by rMLST and it confirmed the MALDI-TOF MS’ results. Only non-pathogenic Y. enterocolitica biotype 1A strains were found, although with a non-negligible prevalence (P = 0.15 with CI 95% = ± 0.06). This study indicates a poor circulation of pathogenic Y. enterocolitica in food products made and marketed in the investigated areas. However, the small number of samples, insufficient for some food categories such as meat and vegetable, does not allow to exclude the presence of pathogenic strains at all.
APA, Harvard, Vancouver, ISO, and other styles
35

Eid, Samah, Sherif Marouf, Hefny Y. Hefny, and Nayera M. Al-Atfeehy. "Pasteurellaceae members with similar morphological patterns associated with respiratory manifestations in ducks." December-2019 12, no. 12 (2019): 2061–69. http://dx.doi.org/10.14202/vetworld.2019.2061-2069.

Full text
Abstract:
Aim: A total of 112 freshly dead ducks aged from 2 to 20 weeks old with a history of respiratory manifestations were investigated for the implication of Pasteurellaceae family members.. Materials and Methods: Isolation and identification to the family level were conducted by conventional bacteriological methods, including microscopic examination and biochemical characterization. Identification to the species level was conducted by polymerase chain reaction (PCR) and analytical profile index (API) 20E kits. Results: Conventional bacteriological isolation and biochemical characterization revealed the infection of 16/112 examined birds with a prevalence rate of 14.3%. PCR confirmed the detection of Pasteurellaceae family conserved genes RpoB and Bootz in 16/16 (100%) isolates. PCR was also used for genus and species identification of the isolated Pasteurellaceae members; the results revealed that 5/16 (31.3%) of isolates were Gallibacterium anatis and 2/16 of isolates (12.5%) were Pasteurella multocida. Riemerella anatipestifer, Mannheimia haemolytica, and Avibacterium paragallinarum were not detected by PCR. Biotyping by API 20E successfully identified 5/16 (31.3%) isolates that could not be typed by PCR and confirmed their belonging to Pasteurella pneumotropica. Neither the available PCR primer sets nor API 20E succeeded for species identification of 4/16 (25%) isolates. Antibiotic susceptibility profiling of isolates revealed that 16/16 (100%) of isolates demonstrated multidrug resistance (MDR) phenotypes. Moreover, 16/16 (100%) of isolates demonstrated a phenotypic resistance pattern to neomycin. Conclusion: Combined genotypic, phenotypic, biotyping, and virulence characterizations are required for laboratory identification of pathogenic Pasteurellaceae. Moreover, P. multocida was not the prevailed member implicated in respiratory problems in ducks as P. pneumotropica, G. anatis, and unidentified strains were involved with higher prevalence. Chloramphenicol and ampicillin demonstrated the highest in vivo effects on the studied Pasteurellaceae. Furthermore, the prevalence of multidrug-resistant isolates signified the demand to implement targeted surveillance in the ducks' production sector, and MDR survey in poultry sectors in Egypt to apply effective control measures.
APA, Harvard, Vancouver, ISO, and other styles
36

Mahdi, Hussain AL-Aammar, Abdzaid Abdali Safaa, and Dakhil Alnasrawy Waleed. "Conventional Versus Molecular Methods for Diagnosis of Burkholderia cepacia from Different Clinical Samples of Iraqi Patients." Biomedicine and Chemical Sciences 1, no. 2 (2022): 57–64. https://doi.org/10.48112/bcs.v1i2.94.

Full text
Abstract:
The study aims at isolating and identification of&nbsp;<em>Burkholderia cepacia</em>&nbsp;bacteria from clinical samples from various pathological conditions such as diabetic foot ulcers, burn, wound, sputum and urine. The present study includes 280 samples collected from patients suffering from diabetic foot ulcer, cystic fibrosis, burns, sputum, and wounds who attend Alsader Medical City and Al-Hakim General Hospital during the period extended from September 2020 to February 2021 ( men and women ) with age groups between (1 -75) years. The identification of bacterial isolates were detected by classical and molecular technique (PCR), where the frequency among males 213 (79.2%) was more than that in female 67 (20.8%). The samples distribution is made according to age group; it appears high for the following high 26.8% with group (31-45) years male and female; 22% with group (31-45) years in male and 7.6% with the group (1-15) years in female. The results revealed that 42/80 specimens of the total number of samples are&nbsp;<em>Burkholderia cepacia</em>&nbsp;by using&nbsp;<em>16SrRNA</em>&nbsp;gene to differentiate the&nbsp;<em>B. cepacia</em>&nbsp;from other (G-) bacteria, and 30/80&nbsp;<em>recA</em>&nbsp;gene to differentiated&nbsp;<em>B. cepacia</em>&nbsp;from&nbsp;<em>B. cepacia</em>&nbsp;complex.
APA, Harvard, Vancouver, ISO, and other styles
37

Abbott, Sharon L., Lourdes S. Seli, Michael Catino, Michael A. Hartley, and J. Michael Janda. "Misidentification of Unusual AeromonasSpecies as Members of the Genus Vibrio: a Continuing Problem." Journal of Clinical Microbiology 36, no. 4 (1998): 1103–4. http://dx.doi.org/10.1128/jcm.36.4.1103-1104.1998.

Full text
Abstract:
Two unusual cases of Aeromonas infection are described, one associated with bacteremia (Aeromonas schubertii) and another in which the organism was recovered from an infected gall bladder (Aeromonas veronii biotype veronii). These strains were initially identified as Vibrio damsela andVibrio cholerae by the Vitek and API 20E systems, respectively. Use of appropriate screening tests and familiarity with the newer Aeromonas species could prevent initial misidentifications and potential public health consequences.
APA, Harvard, Vancouver, ISO, and other styles
38

Kühn, I., G. Huys, R. Coopman, K. Kersters, and P. Janssen. "A 4-year study of the diversity and persistence of coliforms and Aeromonas in the water of a Swedish drinking water well." Canadian Journal of Microbiology 43, no. 1 (1997): 9–16. http://dx.doi.org/10.1139/m97-002.

Full text
Abstract:
Coliforms and Aeromonas, isolated over a sampling period of 4 years from a Swedish drinking water well, were analysed for their phenotypical diversity and for their ability to persist in the well water. From each of the 40 water samples collected from the well, 32 bacterial isolates were subjected to typing by the PhenePlate (PhP) biochemical fingerprinting system. Strains able to persist in the well water were further characterized using the API 20E system, gas–liquid chromatographic cellular fatty acid analysis, and the DNA fingerprinting technique AFLP. Using the PhP system, a total of 170 different phenotypes were identified among 1143 studied isolates. Most phenotypes were only represented by a few isolates and (or) were restricted to one or two sampling occasions. However, one particular phenotype (RV-C01), identified as Aeromonas hydrophila using the API 20E system and fatty acid analysis, reoccurred in 28 samples distributed over the whole study period and often dominated the bacterial population in the well water. AFLP analysis revealed that the RV-C01 isolates displayed basically identical fingerprints. Our results thus suggest that a genetically stable Aeromonas clone resided in the well water over the whole 4-year study, whereas other bacterial strains studied were only transient inhabitants of the well.Key words: Aeromonas, coliform, water, diversity.
APA, Harvard, Vancouver, ISO, and other styles
39

Kusumaningsih, Purwaningtyas. "EVALUATION ON METHODS FOR IDENTIFICATION OF VIBRIO SP.: KAWAKAWA (EUTHYNNUS AFFINIS) BRINING SHREDDED AND CHITOSAN ADDITION AS PRESERVATIVE." BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) 8, no. 1 (2021): 103–13. http://dx.doi.org/10.31289/biolink.v8i1.5011.

Full text
Abstract:
The kawakawa (Euthynnus affinis) brine salting that used to make shredded were preservative with salt. Salt addition has aim to inactivate of bacterial contamination. Obviously, bacteria is still capable of growing in kawakawa brine shredding Therefore, in this study chitosan was added as antibacterial in shredded processing. Vibrio sp., is one of common halophilic bacteria found in seafood. If this bacteria is consumed, it can cause serious problems in human health. The objective of this study was to evaluate the factors that affect in identifying Vibrio sp., on kawakawa brine shredding (C), shredded non-chitosan (FC) and shredded contain of chitosan (FC+). The methods evaluated were steps in enriching bacteria, culturing bacterial in selective media and analysing bacterial by API 20E kit. Enriching and incubation periods were needed by halophilic bacteria to adaptation in new environment. It was required to observe the bacteria characteristics' that would be isolated. Bacterial colonies were growth on Thiosulphate citrate bile salt sucrose (TCBS) were not Vibrio sp., but confirmed as Pseudomonas luteola and Proteus vulgaris based on API 20E analysis. It was showed that TCBS media had some advantages in identifying Vibrio sp. In conclusion, to get the best result in identifying bacteria, at least two or more methods were used to avoid misidentification.
APA, Harvard, Vancouver, ISO, and other styles
40

DJAHNIT, Nora, Housseyn OTMANI, Ryhane LOUNAS, et al. "ÉVALUATION DE LA QUALITÉ MICROBIENNE DE L'EAU DE MER ET DES MOULES D'UNE FERME CONCHYLICOLE ALGÉRIENNE." Geo-Eco-Marina 29 (2023) (December 31, 2023): 039–50. https://doi.org/10.5281/zenodo.10350400.

Full text
Abstract:
Seafood, particularly bivalve mollusks, are eaten raw or undercooked, making them a potential risk of food poisoning. Moreover, these animals filter water and concentrate microorganisms and toxins. This study considers the microbial quality of seawater and mussels sampled from a shellfish farm on the Algerian coast. Samples were investigated by enumeration methods for the fecal indicators and the research of pathogens such as Staphylococcus and Salmonella. Strains of interest have been characterized and then identified with API 20E test galleries following the manufacturer's instructions. The seawater's microbiological results revealed that the levels of fecal coliforms were lower than the allowed limit (&lt;500&nbsp;CFU/100&nbsp;ml) in all the stations during the study period. However, Staphylococcus aureus and Salmonella spp.&nbsp;were positive in all stations. Regarding mussel samples, the results showed that they were highly contaminated by fecal coliforms (&gt;300 CFU/100 g). Similarly to seawater's results, we detected the presence of Staphylococcus aureus and Salmonella spp.&nbsp; Isolate's identification by API 20E test galleries revealed the presence of the same species Salmonella enterica ssp Arizona in seawater and mussels' samples. When considering the indicators and bacterial pathogens investigated, the mussels examined were of bad microbiological quality, which can threaten consumer health.
APA, Harvard, Vancouver, ISO, and other styles
41

Munther Al Agha, Abeer Ghassan, Nazar Jabar Muslih Al Khafaji, and Amer Khazal Salih Al Azawi. "Isolation and Identification of Klebsiella pneumoniae using API-20E analytical system and conventional PCR assay." International Journal of Current Microbiology and Applied Sciences 6, no. 8 (2017): 203–10. http://dx.doi.org/10.20546/ijcmas.2017.608.028.

Full text
APA, Harvard, Vancouver, ISO, and other styles
42

Arcuri, Edna Froeder, Priscilla Diniz Lima da Silva, Maria Aparecida Vasconcelos Paiva Brito, José Renaldi Feitosa Brito, Carla Christine Lange, and Margarida Maria dos Anjos Magalhães. "Contagem, isolamento e caracterização de bactérias psicrotróficas contaminantes de leite cru refrigerado." Ciência Rural 38, no. 8 (2008): 2250–55. http://dx.doi.org/10.1590/s0103-84782008000800025.

Full text
Abstract:
Com os objetivos de quantificar, isolar e caracterizar bactérias psicrotróficas contaminantes de leite cru refrigerado, produzido na região da Zona da Mata de Minas Gerais e Sudeste do Rio de Janeiro, foram analisadas amostras de leite coletadas de 20 tanques coletivos e 23 tanques individuais. As contagens de bactérias psicrotróficas nas amostras de leite para os dois tipos de tanques de refrigeração variaram entre 10² e 10(7) Unidades Formadoras de Colônias (UFC) ml-1, porém, um maior número de tanques coletivos apresentou contagens acima de 1 x 10(5) UFC ml-1. Foi verificada a predominância de bactérias psicrotróficas gram-negativas (81,2%), que foram identificadas pelos sistemas API 20E e API 20NE nos gêneros: Aeromonas, Alcaligenes, Acinetobacter, Burkholderia,Chryseomonas, Enterobacter, Ewingella, Klebsiella, Hafnia, Methylobacterium, Moraxella, Pantoea, Pseudomonas, Serratia, Sphingomonas e Yersinia. As bactérias gram-positivas (18,8%) foram identificadas com API 50 CH, API Coryne e API Staph, nos gêneros: Bacillus, Brevibacterium, Cellum/Microbacterium, Kurthia e Staphylococcus. Os sistemas API utilizados não identificaram todos os isolados bacterianos. Pseudomonas foi o gênero mais isolado e P. fluorescens foi a espécie predominante. A maioria dos isolados bacterianos apresentou atividade proteolítica e/ou lipolítica a temperaturas de refrigeração de 4°C, 7°C e 10°C, evidenciando seu alto potencial de deterioração do leite e dos produtos lácteos. Os resultados ressaltam que maior atenção deve ser dada aos procedimentos que impeçam a contaminação do leite por esses microrganismos.
APA, Harvard, Vancouver, ISO, and other styles
43

Kozak, Sergey. "Improvement of method for Proteus bacteria genus detection in poultry slaughtering products." Poultry and Chicken Products 25, no. 6 (2023): 31–34. http://dx.doi.org/10.30975/2073-4999-2023-25-6-31-34.

Full text
Abstract:
Possibilities of Proteus bacteria genus detection have been considered in poultry slaughtering products with the next methods: selective medium for bacteria genera Proteus, Morganella, Providencia; differential diagnostic agar (DDA) for these bacteria detection; biochemical plates for enterobacteria differential (PBDE); test systems API 20E and MMT E4 multi micro test for enterobacteria biochemical identification. The new high sensitive method gives the pos- sibility to reduce detection durability of Proteus bacteria from 9 days (in accordance with 7702.2.7-2013 State Standard)
APA, Harvard, Vancouver, ISO, and other styles
44

Hasan, Thamer O., Inam J Lafta, Emad A Ahmed, and Samah A Jassam. "Application of RAPD-PCR and Phylogenetic Analysis for Accurate ‎Characterization of ‎Salmonella‎‎‎ spp. Isolated from Chicken and Their Feed ‎and Drinking Water in Comparison ‎with API 20E, Vitek 2, and Serotyping." Iraqi Journal of Veterinary Medicine 47, no. 1 (2023): 11–20. http://dx.doi.org/10.30539/ijvm.v47i1.1493.

Full text
Abstract:
&#x0D; &#x0D; &#x0D; &#x0D; The aim of this study was ‎the‎ discrimination of Salmonella‎‎ isolated from chicken and their feed ‎and drinking water for the epidemiological control of salmonellosis. Totally, 289 samples, ‎including 217 chicken cloaca swabs, 46 water, and 26 feed samples were collected from five ‎different farms in Karbala governorate, Iraq. Conventional bacteriology tests, API 20E, Vitek 2, ‎and serology were used for bacterial identification. Random amplified polymorphic ‎DNA (RAPD)-polymerase chain reaction (PCR) was applied to analyze the genetic relationships ‎among Salmonella‎‎ isolates. The isolation rate of Salmonella‎‎ spp. was 21.1% (61/289). While the ‎water samples constituted the highest rate (30.4%), a rate of 21.7% was reported for the cloaca ‎swabs, with no isolate at all from chicken feed. Vitek 2 was able to identify some isolates to the ‎serotype level, such as S. Enteritidis, S. Paratyphi B, and S. Paratyphi C. However, the isolates ‎were diagnosed as S. enterica by API 20E, and as S. enterica subsp. arizonae through serology. ‎Analyzing the samples by the RAPD-PCR assay showed the presence of genetically different ‎Salmonella‎‎ spp. Dendrograms created by the GelJ software successfully delineated the genetic ‎relationships. Therefore, RAPD-PCR can be used as a surrogate tool for the fast, reliable, and ‎accurate detection of Salmonella‎‎ in epidemiological surveys when compared with other ‎biochemical-based identification methods.&#x0D; &#x0D; &#x0D; &#x0D;
APA, Harvard, Vancouver, ISO, and other styles
45

Aamer Habeeb, Thuraya, Adnan H. Al-Hamadani, and Jawad K. AL-Janabi. "The use of Omp W Gene in Detection of Vibrio cholerae Isolated from Diarrhea Cases of Children in AL-Diwaniya Province." AL-QADISIYAH MEDICAL JOURNAL 6, no. 9 (2017): 185–96. http://dx.doi.org/10.28922/qmj.2010.6.9.185-196.

Full text
Abstract:
A total of 221 stool samples were collected from children suffering from watery diarrhea, less than 15 years old of both genders whom admitted to the Maternity and Children Teaching Hospital in Al- Diwaniya Province, In addition to that, 27 water samples were also collected from three different loci of ALDiwaniya River, at the period of October 2008 to June 2009,in order to evaluate the routine laboratory diagnostic procedures in the diagnosis the multi-serogroups or serotype of Vibrio cholerae strains and compared them with molecular technique as Polymerase chain reaction (PCR).Vibrio cholerae has been isolated and identified by using culturing method in addition to biochemical tests, API 20E diagnostic kit. Serotyping by using polyvalent Vibrio cholerae, O1antisera, and monovalent Ogawa and Inaba revealed that the most of clinical V. cholerae were of serogroup O1, while all the V. cholerae isolated from surface water of AL- Diwanyia river was Non-O1 serogroup. PCR technique was used to detect ompW gene encoding to outer membrane protein of V. cholerae. Based on the PCR results, the rate of Vibrio cholerae isolation from stool samples was 5.9%. PCR results showed that there were high specificity (100%, 100%, 97% and 86%) in detection of Vibrio cholerae strains versus each of cultural, biochemical, API 20E system and serological tests, respectively.
APA, Harvard, Vancouver, ISO, and other styles
46

Zghair, Zainab Razzaq, and Waleed Khalid Mathree Waleed Khalid Mathree. "Molecular characteristics of Pseudomonas aeruginosa isolated from human sources and chickens." University of Thi-Qar Journal of agricultural research 14, no. 1 (2025): 62–72. https://doi.org/10.54174/r4y2ab85.

Full text
Abstract:
Samples were collected from different locations in Wasit Governorate, Iraq, 120human samplesTested from ear swabs, wounds, burns, feces and 153 chicken samplesP. aeruginosa isolates. Samples were striped on blood andMcConkey agarSuspect colonies are grown on chromogenic media and surrounded by citram. She was isolated The determination is made according to colony morphology, Gram staining, and conventional biochemistry Interactions and analysis profile of Index 20E (API 20E). A PCR examination confirmed the presence of P. aeruginosa in 11.35%. (273/31), 17.5% ( 120/21) from human samples, and 6.53% (153/10) from chickens.The polymerase chain reaction demonstrated that 100% of the isolates were P.Aeruginosa and all human and chicken isolates carry the exoA and exoT gene and the mexR gene, at a percentage (100%). And the study of histopathology on the internal organs (heart, lung, liver and intestine) of infected chickens.
APA, Harvard, Vancouver, ISO, and other styles
47

Vantomme, R., C. Rijckaert, J. Swings, and J. de Ley. "Characterization of Further Erwinia amylovora Strains and the Application of the API 20E System in Diagnosis." Journal of Phytopathology 117, no. 1 (1986): 34–42. http://dx.doi.org/10.1111/j.1439-0434.1986.tb04357.x.

Full text
APA, Harvard, Vancouver, ISO, and other styles
48

Overman, T. L., and J. K. Overley. "Feasibility of same-day identification of members of the family Vibrionaceae by the API 20E system." Journal of Clinical Microbiology 23, no. 4 (1986): 715–17. http://dx.doi.org/10.1128/jcm.23.4.715-717.1986.

Full text
APA, Harvard, Vancouver, ISO, and other styles
49

Tri, Yahya Budiarso, Amarantini Charis, Restiani Ratih, et al. "Isolation and Biochemical Characterization of Enterobacter cloacae Isolates from Ready-to-eat Foods Using API 20E." INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGICAL SCIENCES 5, Sep & Oct 2021 (2021): 24–30. https://doi.org/10.5281/zenodo.5734017.

Full text
Abstract:
<em>Ready-to-eat foods have become a global concern because they are a source of income for low and middle-income people. Also, they constitute the main food for students and lower to middle-level workers. However, a lack of knowledge about good food processing practices results in microbial contamination that interferes with human health. Enterobacter cloacae is one of the most common bacterial contaminants found in ready-to-eat foods in many countries. Therefore, the purpose of this research is to identify E. cloacae using the API 20E kit on ready-to-eat foods in Yogyakarta city and its surroundings. Subsequently, 115 samples of 10 types of food were collected from various locations for isolation and identification of the contaminant. The results showed that 7 foods were found to be contaminated, with processed egg products having the highest contamination level at 40%. This was followed by various snacks and packaged dairy products at 30% each, then skewered meatballs, and other processed food products, at 20% and 10%, respectively. Meanwhile, dumplings, potato products, and assorted iced drinks were not contaminated by the bacteria</em>
APA, Harvard, Vancouver, ISO, and other styles
50

Al-Mossawi, Hesnaa, Jabbar Hassan, Nada Al-bashier, and Areej AlOmrani. "IDENTIFICATION COMMON CAUSE OF NEONATAL SEPSIS BY ANALYTICAL PROFILE INDEX SYSTEM (API)." Iraqi Journal of Medical Sciences 16, no. 3 (2018): 327–34. http://dx.doi.org/10.22578/ijms.16.3.12.

Full text
Abstract:
Background: Neonatal Sepsis is a bacterial infection of the blood in a neonate and an infant younger than 4 weeks of age. The analytical profile index or API is a classification of bacteria based on experiments, allowing fast identification. This system is developed for quick identification of clinically relevant bacteria. Objective: To identify the pattern of organisms in neonatal sepsis using API system in Baghdad City hospital, Al-Imamein Al-Kadhimein Medical City and Central Pediatrics Teaching Hospital. Methods: In this cross-sectional study, blood samples from 100 neonatal patients were inoculated into a blood culture bottle and incubated at 37 °C under aerobic conditions, subculture was done after 24 h of incubation, the growth was identified by phenotypic characteristics, gram's stain, and API system. Results: Positive blood cultures were detected in 82 (82%), according to API system the most prominent bacterial isolates from blood culture in neonates with early-onset sepsis were non-coagulase Staphylococcus (20.4%) Staphylococcus aureus (18.1 %), Acinetobacter bumanii (13.6%), Pseudomonas aeruginosa (11.36 %), Streptococcus agalactiae (11.3 %), while in late-onset sepsis the most common bacteria were Staphylococcus aureus (21.0%), non-coagulase Staphylococcus (13.1%), Citrobacter freundii and Pseudomonas aeruginosa (10.5 %) respectively. Conclusion: The API 20E may be useful for the identification of the bacterial species rarely described as pathogens in neonatal sepsis will help us to study the clinical burden resulting from the emergence of these species as causes for this neonatal infection. Keywords: Early-onset sepsis, late-onset sepsis, blood culture, API system Citation: Al-Mossawi HSM, Hassan JS, Al-bashier NM, Al-Omrani AAA. Identification common cause of neonatal sepsis by analytical profile index system (API). Iraqi JMS. 2018; 16(3): 327-334. doi: 10.22578/IJMS.16.3.12
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography