Relevant bibliographies by topics / Applied microbiology

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Applied microbiology'

Author: Grafiati
Published: 4 June 2021
Last updated: 27 January 2023

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Journal articles on the topic "Applied microbiology"

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Mulhall, A. "Applied microbiology." International Journal of Nursing Studies 27, no. 1 (January 1990): 95–96. http://dx.doi.org/10.1016/0020-7489(90)90028-h.

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Sanderson, P. J. "Applied microbiology." Journal of Hospital Infection 15, no. 4 (May 1990): 399–400. http://dx.doi.org/10.1016/0195-6701(90)90102-t.

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Lafon-Lafourcade, S. "Applied microbiology." Experientia 42, no. 8 (August 1986): 904–14. http://dx.doi.org/10.1007/bf01941767.

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Jelen, P. "Applied dairy microbiology." International Dairy Journal 10, no. 8 (January 2000): 586. http://dx.doi.org/10.1016/s0958-6946(00)00079-0.

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Onishi, Yasuo. "Biotechnology in Applied Microbiology." TRENDS IN THE SCIENCES 8, no. 12 (2003): 64–65. http://dx.doi.org/10.5363/tits.8.12_64.

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Eydmann. "Applied Microbiology P Caddow , Applied Microbiology Scutari 262pp £9.95 1-871364-08-6." Nursing Standard 4, no. 24 (March 7, 1990): 53. http://dx.doi.org/10.7748/ns.4.24.53.s55.

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Beppu, Teruhiko. "An answer from applied microbiology." TRENDS IN THE SCIENCES 4, no. 3 (1999): 26–29. http://dx.doi.org/10.5363/tits.4.3_26.

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Sauter, Thomas. "New trends in applied microbiology." Trends in Biotechnology 20, no. 1 (January 2002): 43. http://dx.doi.org/10.1016/s0167-7799(01)01870-4.

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Sonnleitner, B. "General, molecular and applied microbiology." Journal of Biotechnology 5, no. 3 (May 1987): 233–34. http://dx.doi.org/10.1016/0168-1656(87)90020-4.

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Pramer, D. "Future impacts of applied microbiology." MIRCEN Journal of Applied Microbiology and Biotechnology 2, no. 1 (1986): 177–90. http://dx.doi.org/10.1007/bf00937192.

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Dissertations / Theses on the topic "Applied microbiology"

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Dabdoub, Shareef Majed. "Applied Visual Analytics in Molecular, Cellular, and Microbiology." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1322602183.

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Iker, Brandon Charles. "Application of Advanced Molecular Techniques in Applied Environmental Microbiology." Diss., The University of Arizona, 2013. http://hdl.handle.net/10150/301699.

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Recent advancements in molecular biology such as next generation sequencing and more sensitive and rapid molecular detection methods like qPCR, have historically been developed for clinical applications in human genetics and for health care diagnostic purposes. The high demand for faster and more accurate molecular assays in the health care field has driven rapid development of inexpensive molecular techniques that when applied to the science of environmental microbiology, provides an unprecedented level of understanding of the microbial world around us. The goal of this dissertation is to begin to apply more advanced molecular technologies to problems in applied environmental microbiology. Appendix A is a brief literature review of next generation sequencing technologies for applications in environmental microbiology. Appendix B focuses on the development of a more robust virus nucleic extraction kit for the detection of viral genomes from environmental samples found to contain high concentrations of qPCR inhibitors, such as humic acids or heavy metals. Appendix C summarizes one of the largest virus surveys done in the US, using state of the art qPCR technologies in both wastewater influent and effluent from two wastewater treatment plants in the Southwest. Data suggests that traditional virus indicators may not be a viable tool to evaluate fecally impacted source water or virus removal during water treatment. The third study summarized in Appendix D, provides one of the first insights into the microbial ecology of biofilms utilized as biological treatment media using Roche 454 amplicon sequencing of the 16S rRNA gene.
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Castro, Lilian Cristina Menegon. "Avaliação de indicadores biológicos na validação de processos de esterilização de isoladores por peróxido de hidrogênio." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/9/9139/tde-14062016-184137/.

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A resistência de cinco microrganismos presentes na microbiota da área de produção estéril (Cristalização Estéril), frente a ação do gás de peróxido de hidrogênio foi determinada e o valor O obtido para cada microrganismo foi comparado ao valor D do Bacillus stearothermophilus ATCC 12980 exposto ao mesmo agente. Os microrganismos testados foram Bacillus sp, M. luteus, Corynebacterium, Staphylococcus sp e Penicillium sp. Este teste tinha a finalidade de comprovar que a resistência do Bacillus stearothermophilus é maior quando da exposição ao peróxido de hidrogênio se comparada a outros microrganismos presentes na área produtiva. A metodologia consistiu da inoculação de 0,01 mL da suspensão de cada microrganismo na contagem de 102UFC/0,01 mL em cupons de aço inoxidável, previamente esterilizados por calor seco e posterior exposição ao gás de peróxido de hidrogênio. O experimento demonstrou que o valor D obtido para o Bacillus stearothermophilus ésuperior aos obtidos para os outros microrganismos em teste comprovando que a escolha deste microrganismo para o desafio contra o peróxido de hidrogênio é apropriada. Também executou-se o teste que visava garantir que o aço inoxidável é o material de suporte mais recomendado para este fim, utilizando-se suportes de diversos materiais normalmente encontrados no interior dos isoladores (PVC, aço inoxidável, CKC, teflon, polipropileno, látex, silicone, Hypalon, vidro, nylon, saco de alumínio) com 0,01 mL de inóculo de Bacillus stearothermophilus na contagem de 102UFC/O,01 mL, o que foi devidamente comprovado.
The resistance of tive microrganisms found in the sterile production area (Crystallization Area) flora was tested against the hydrogen peroxide gas and the D value of each microrganism was compared to the Bacillus stearothermophilus D Value ATCC 12980 exposed to the same agent. The microrganisms tested were Bacillus sp, M. luteus, Corynebacterium, Staphylococcus sp e Penicillium sp. The purpose of this test was to prove that the resistance of Bacillus stearothermophilus against the exposition the hydrogen peroxide is higher when compared to others microrganisms found in the production area. The methodology consisted in inoculating 0.01 mL of microrganisms suspension with 102UFC/0,01 mL count in stainless steel coupons, treated previously with dry heat and further exposition to the hydrogen peroxide. The experiment demonstrated that the Bacillus stearothermophilus D value is higher against all others microrganisms tested proving that the use of this microrganism for the challenge is appropriate. It was also pertormed a test to guarantee that the stainless steel support is the most recommended one for this purpose, using supports of different materials normally found in the interior of the isolators (PVC, stainless steel, CKC, Teflon, polypropylene, latex, silicon, Hypalon, glass, nylon, aluminum foil) with 0,01 mL Bacillus stearothermophilus inoculum with the count of 102UFC/O,01 mL, that was properly veritied.
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Lennox, Samuel David. "The applied mathematical modelling of milk and milk solids production." Thesis, Queen's University Belfast, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317476.

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Malactos, Michael D. "Investigations into the detection of injured Salmonella typhimurium in foodstuffs." Thesis, University of Bedfordshire, 1998. http://hdl.handle.net/10547/336486.

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A chemically defined medium for Salmonella growth was developed and optimised using supplements of amino acids, nucleosides, vitamins and different carbon sources. The medium developed was compared to commercially available pre-enrichment media BPW and Salmosyst. Growth of Salmonella was significantly higher in BPW and Salmosyst than the medium developed. The amino acid and nucleoside supplement was directly compared with peptone. The results show peptone to be nutritionally superior and promoting better growth of Salmonella. Three different ELISA assays were used to detect Salmonella growing in four different media. The ELISA assay sensitivity was determined and a degree of media interference with the immunoassays was established. Salmonella culture viability was investigated using three different procedures: differential culturing on selective and non-selective media; fluorescence microscopy with BACLIGHT stained cells and flow cytometry analysis of BACLIGHT and BEP stained cells. Flow cytometry was found to be the most consistent, sensitive and rapid procedure for cell viability measurement. Clusters of viable cells unable to grow on solid media and therefore remaining undetectable by cultural methods were identified using flow cytometry. Severely heat injured Salmonella was used to determine media recoverability. The results indicate that media which contain peptone recover injured Salmonella better than chemically defined or other media. Detection of Salmonella was performed using PCR assay after sample pre-enrichment. The amplification of Salmonella DNA extracted using a crude method resulted in an assay sensitivity of 20 Salmonella cells in pure cultures. The specificity of the oligonucleotide primers employed in the PCR assay was confirmed. Non-salmonella organisms present in high numbers interfered with PCR detection of Salmonella. Food components also interfered with PCR amplification and reduced the assay sensitivity. Interference by food components and non-salmonella DNA was eliminated by the use of a 24 hour pre-enrichment followed by a 3 hour secondary enrichment, a rapid DNA extraction and template preparation. Using this system it was possible to detect 3 Salmonella cells per gram of food in the presence of 106 non-salmonella cells within 28 hours.
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Vila, Brugalla Montserrat. "Microbiological safety evaluation of sous-vide treatments at mild temperatures applied to pork loin." Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/667333.

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Actualment s’observa un increment en la utilització de tècniques de cocció a temperatures moderades com la cuina al buit. Donada la possibilitat d’utilitzar aquestes tècniques per a preparar menjars amb antelació i considerant que les dades sobre el comportament microbià en el rang entre 40 i 60 ºC són escasses, hi ha un gran interès en definir la seva seguretat microbiològica. Aquesta tesi doctoral té com a objectiu avaluar l’efecte de la cocció al buit a temperatures moderades sobre L. monocytogenes i Salmonella spp. inoculades en llom de porc. Es van realitzar assajos d’inoculació (challenge test) en llom de porc, amb dues soques de Salmonella (S. enterica subsp. enterica serovar Enteritidis CECT 4300 i Senftenberg CECT 4565) i Listeria monocytogenes (CECT 4031 i Scott A), avaluades de forma individual o combinada. Les porcions de carn inoculada es van envasar al buit i es van cuinar en un forn de vapor a dues temperatures (55 i 60 ºC) durant 30, 60 o 90 minuts. Es va determinar la letalitat i la recuperació microbiana durant l’emmagatzematge a 4 i 8 ºC fins al dia 30 post-cocció. L’evolució en el recompte es va modelitzar utilitzant les eines predictives Bioinactivation FE per a la inactivació tèrmica i DMFit pel creixement durant l’emmagatzematge. Es va aplicar el mètode Monte Carlo per incorporar la variabilitat observada en els models predictius. L’heterogeneïtat dels resultats va ser important, i una de les seves fonts va ser probablement el funcionament del forn a les temperatures d’assaig. En general, Salmonella spp. es mostrà més termoresistent que L. monocytogenes, però el seu creixement durant l’emmagatzematge va ser més limitat. Per tant, l’avaluació de la seguretat microbiològica d’aquests tractaments moderats ha de considerar tant la letalitat com la capacitat de recuperació durant l’emmagatzematge. Tenint en compte tots dos factors, es van obtenir tres escenaris diferents. El primer vas ser d’inactivació completa (S. Enteritidis i L. monocytogenes Scott A i CECT 4031 a 60 ºC) és a dir, no es van detectar les soques inoculades en cap rèplica durant tot el període d’emmagatzematge. L’escenari de viabilitat es va observar en casos en què totes les rèpliques van mostrar recomptes superiors al límit de quantificació (5 CFU/g), amb o sense creixement (S. Senftenberg, L. monocytogenes Scott A, còctel de soques de L. monocytogenes a 55 ºC i còctel de soques de Salmonella spp. a 55ºC durant 30 min). Finalment, l’escenari de "creixement/no creixement", es va caracteritzar per diferents proporcions de mort, viabilitat i lesió a nivell cel·lular. Aquesta circumstància es va produir en mostres inoculades cuinades a 60 ºC (S. Senftenberg, còctel de L. monocytogenes) i 55 ºC (còctel de Salmonella spp. i L. monocytogenes CECT 4031). En conclusió, la seguretat microbiològica dels processos de cocció al buit a temperatura moderada ha de ser curosament avaluada cas per cas. El nostre estudi indica que els tractaments a 60 ºC durant 90 minuts aplicats al llom de porc es podrien considerar raonablement segurs, sempre que no es produeixi un abús de temperatura durant la vida útil. Els tractaments a 60 ºC durant 30 i 60 minuts es podrien utilitzar només en cas de servei immediat després de la cocció. La cocció de llom de porc a 55 ºC durant 30, 60 i 90 minuts no es pot considerar segura en relació a S. Senftenberg i L. monocytogenes Scott A, donat que les cèl·lules supervivents poden créixer a 8 ºC. Aquest tractament s’ha d’utilitzar només per a sistemes de servei immediat. Aquest estudi proporciona dades útils per a futures avaluacions del risc aplicades a la cocció al buit de carn a temperatures moderades.
A raising interest has been detected nowadays in relation to the use of cooking techniques at moderate temperatures like sous-vide cooking. Given the possibility of using these techniques to prepare food in advance, and considering that data on behaviour of bacteria in the range of 40 to 60 °C is scarce, there is great concern in accurately define the microbiological safety of this food. The aim of this doctoral thesis was to characterize the effect of mild temperature vacuum cooking treatments on two main food pathogens, L. monocytogenes and Salmonella spp. inoculated in raw pork meat. Challenge studies were conducted using two bacterial strains of Salmonella (S. enterica subsp. enterica serovar Enteritidis CECT 4300 and Senftenberg CECT 4565), and Listeria monocytogenes (CECT 4031 and Scott A), inoculated either individually or in combination. Pork loin pieces were inoculated, vacuum packed and cooked in a steam oven at two different temperatures (55 and 60 ºC) during 30, 60 or 90 minutes. Lethality caused by each treatment was determined just after cooking. Further recovery of injured cells was evaluated during storage at 4 and 8 ºC until the 30th day after treatments. Evolution of microbial counts were modelled using the predictive tools Bioinactivation FE for thermal inactivation and DMFit for growth through the storage time. The Monte Carlo method was applied in order to incorporate the variability observed between replicates in the predictive models of inactivation and growth. The heterogeneity of results was important. One source of this variability was probably the steam oven performance between 55 and 60 ºC. At these cooking temperatures, Salmonella spp. was more heat resistant than L. monocytogenes, but it was less able to growth during cold storage. Microbiological safety evaluation of sous-vide mild heat treatments must be based both on lethality and on the capability of recovery during storage. Considering both factors, three different scenarios were obtained: complete inactivation, presence of viable cells and a growth/no growth interface. In case of a complete inactivation (S. Enteritidis and L. monocytogenes Scott A and CECT 4031 cooked at 60 ºC), inoculated strains were not detected in any replicate during all the storage period. In case of viability, all replicates showed counts above the quantification limit (5 CFU/g), whether growth was observed or not (S. Senftenberg, L. monocytogenes Scott A, cocktail of L. monocytogenes strains at 55 ºC, and cocktail of Salmonella spp. strains at 55 ºC during 30 min). Finally, in some experimental conditions a “growth/no growth” behaviour, with different proportions of death, viable and injured cells, was observed. This circumstance took place in inoculated samples cooked at 60 ºC (S. Senftenberg, cocktail of L. monocytogenes) and 55 ºC (cocktail of Salmonella spp. and L. monocytogenes CECT 4031). In conclusion, the microbiological safety of sous-vide mild heat processes have to be accurately assessed on a case-by-case basis. Heat treatments at 60 ºC for 90 min applied to pork loin could be considered reasonably safe and suitable for “cook-chill” systems in relation to Salmonella spp. and L. monocytogenes strains included in the essay, as long as no temperature abuse occurs during shelf-life. Treatments at 60 ºC during 30 and 60 minutes must be used only for “cook-serve” systems. Sous-vide cooking of pork loin at 55 ºC during 30, 60 and 90 minutes cannot be considered safe in relation to S. Senftenberg and L. monocytogenes Scott A due to the presence of survivor cells that can growth at 8 ºC. This treatment must be used only for “cook-serve” systems. This study provides useful data for future risk assessment studies applied to meat sous-vide cooked at mild temperatures.
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Skipper, Philip. "Biodeterioration of limestone : role of bacterial biofilms and possible intervention strategies." Thesis, University of Lincoln, 2018. http://eprints.lincoln.ac.uk/33697/.

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Limestone built heritage is at risk from the effects of biofilms, a microbial community encapsulated in a matrix of sugars, protein and extracellular DNA. Although biofilm research has been carried out in Mediterranean regions, few studies cover temperate Northern Europe climates, or the UK. This study concentrates on bacterial colonisation of Lincoln limestone, a highly vulnerable building material, and identifies the species, their role in biodeterioration and the efficacy of biocides against them. As part of this study the core species which comprise the bacterial component of the limestone microbiome have been characterised for the first time; this has allowed the identification of non-core species which are significantly associated with damaged and undamaged surfaces. Four mechanisms of biodeterioration have been identified, one previously unidentified, and isolated species have been characterised as to whether they are biodeteriorative and the mechanisms of biodeterioration that they employ. Two species, Curtobacterium flaccumfaciens and Solibacillus silvestris, have been characterised as producing biofilm matrix which actively causes biomechanical damage to the oolitic limestone structure as opposed to the passive enhancement of physical weathering which has been previously associated with biofilm matrix. Species capable of biodeterioration have also been shown to be present on both damaged and undamaged surfaces, something which has not been previously investigated. Environmental sampling, species identification and characterisation of species for biodeterioration have all combined to identify markers of biodeterioration, ie both physical markers and biomarkers. Specifically, a surface pH of 5.5 or lower and the presence of B. licheniformis is indicative of biodeterioration with a proportionally higher level of M. luteus when comparing damaged and undamaged stone. Finally this study brings the literature on conservation methods up to date by testing biocides which are in current usage, as many biocides in the literature are discontinued. This study is also the first in the field to show their efficacy against biofilm encapsulated bacteria and their propensity for chemically disrupting the biofilm matrix.
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Reinert, Cristina. "Caracterização do cassete cromossômico estafilocócico mec (SCCmec) de cepas endêmicas nosocomiais de Staphylococcus aureus resistentes a oxacilina e vancomicina." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-16082017-164142/.

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Os tipos de cassete cromossômico estafilocócico mec (SCCmec) encontrados em cepas de Staphylococcus aureus resistente a oxacilina (ORSA) isoladas em ambiente hospitalar são denominados I, II e III. Um novo SCCmec, tipo IV, foi identificado em cepas isoladas de pacientes que não tinham conexão aparente com hospitais. Essas cepas, denominadas ORSA adquiridas na comunidade ou CA-ORSA, não costumam conter genes de resistência a antimicrobianos além do gene mecA, porém, são mais virulentas. Com a finalidade de conhecer a diversidade estrutural do SCCmec de cepas ORSA brasileiras, foram estudadas 50 cepas ORSA, entre elas algumas S. aureus com resistência intermediária a vancomicina (VISA) e S. aureus com resistência heterogênea intermediária a vancomicina (HVISA). As cepas foram isoladas entre 1995 e 2000, provenientes de hospitais de 13 Estados brasileiros, pertencentes a diversos clones de PFGE. As cepas foram analisadas quanto ao perfil de sensibilidade, RFLP do gene da coagulase, fagotipagem e tipagem do SCCmec. A maioria das cepas é pertencente ao clone endêmico brasileiro, que carrega o SCCmec tipo III, também presente em outros clones de PFGE. O SCCmec tipo IV, encontrado em 3 cepas (clones J, L e D), mostrou suscetibilidade a um maior número de antimicrobianos pertencentes a diferentes classes, em comparação aos outros tipos de SCCmec. O SCCmec tipo IV está presente entre os ORSA brasileiros e pode estar disseminando na comunidade e hospitais do país.
The types of SCCmec found in nosocomial methicillin-resistant Staphylococcus aureus (MRSA) strains can be designated type I, II or III. A novel type of SCCmec, designated type IV, was identified in strains isolated in outpatients. These strains, called community-acquired MRSA or CA-MRSA, do not contain antimicrobial resistance genes other than mecA, however, they are more virulent. In order to characterise the structural diversity of SCCmec in Brazilian MRSA strains, 50 MRSA strains were studied, including some vancomycin intermediate resistant S. aureus (VISA) and heterogeneous vancomycin intermediate S. aureus (HVISA) strains. All strains were isolated between 1995-2000, in hospitaIs in 13 Brazilian States, and belonged to different PFGE clones. Strains were analysed as to their susceptibility profile, RFLP of the coagulase gene, phagetype and SCCmec type. The majority of strains belonged to the Brazilian Endemic Clone, which carries an type IH SCCmec, which in turn, was also found in other PFGE clones. The type IV SCCmec was found in 3 strains (belonging to the J, L and D clones) and presented a susceptibility profile to a number of drugs of different antimicrobial classes. The type IV SCCmec is present among Brazilian MRSA strains and can be disseminated in the community and in hospitaIs throughout the country.
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Visi, David K. "The Microbial Retting Environment of Hibiscus Cannabinus and Its Implications in Broader Applications." Thesis, University of North Texas, 2015. https://digital.library.unt.edu/ark:/67531/metadc801953/.

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Fiber-yielding plants is an area of increased interest due to the potential use in a variety of green-based materials. These biocomposites can be incorporated into multiple uses; for example, to replace building materials and interior vehicular paneling. The research here aims to focus in on the crop Hibiscus cannabinus for utilization into these functions. H. cannabinus is economically attractive due to the entire process being able to be accomplished here in the United States. The plant can be grown in a relatively short growth period (120-180 days), and then processed and incorporated in a biocomposite. The plant fiber must first be broken down into a useable medium. This is accomplished by the retting process, which occurs when microbial constituents breakdown the heteropolysaccharides releasing the fiber. The research aims to bridge the gap between the primitive process of retting and current techniques in molecular and microbiology. Utilizing a classical microbiological approach, which entailed enrichment and isolation of pectinase-producing bacteria for downstream use in augmented microbial retting experiments. The tracking of the bacteria was accomplished by using the 16S rRNA which acts as “barcodes” for bacteria. Next-generation sequencing can then provide data from each environment telling the composition and microbial diversity of each tested variable. The main environments tested are: a natural environment, organisms contributed by the plant material solely, and an augmented version in which pectinase-producing bacteria are added. In addition, a time-course experiment was performed on the augmented environment providing data of the shift to an anaerobic environment. Lastly, a drop-in set was performed using each isolate separately to determine which contributes to the shift in microbial organization. This research provided a much needed modernization of the retting technique. Previous studies have been subject to simple clone libraries and growth plate assays and next-generation sequencing will bring the understanding of microbial retting into the 21st century.
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Zimmermann, Ana Lucia Santos. "Desenvolvimento e avaliação de micropartículas contendo microrganismos viáveis utilizados como bioinseticida." Universidade de São Paulo, 2001. http://www.teses.usp.br/teses/disponiveis/9/9139/tde-09022015-103417/.

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Neste trabalho foi feito um estudo para obtenção de formulações multiparticuladas, formadas por micropartículas de polímeros naturais, solúveis em água, não tóxicos e biodegradáveis. Os polímeros utilizados foram: Caseína, Hidroxietilcelulose (HEC), Hidroxipropilmetilcelulose (HPMC), Alginato de sódio e Quitosana. As técnicas utilizadas para obtenção das micropartículas foram o spray drying e a atomização de alginato de sódio em uma solução de CaCl2, para gelificação das gotículas formadas, com uma complexação ou não com quitosana. O estudo de micropartículas secas de alginato de cálcio e alginato de cálcio recoberta com uma membrana de quitosana revelou que diferentes procedimentos e variáveis de processo influenciavam nas características das micropartículas obtidas. O diâmetro médio das micropartículas de alginato e alginato-quitosana variou de 60 a 553 µm. A superfície de micropartículas com quitosana se mostrou mais rugosa, com uma grande quantidade de poros menores que 1 µm. As micropartículas com diâmetro médio de 60 µm apresentaram uma boa esfericidade e uma distribuição de tamanho de partícula uniforme. O teor de cálcio apresentou variações, diminuindo em processos com quitosana. A maioria das micropartículas de alginato e alginato-quitosana eram estáveis em água, mas instáveis em soro fisiológico e tampão fosfato 0,1M. Após a realização do estudo das características destas micropartículas foram incorporados a elas materiais com atividade bioinseticida (Bacillus thuringiensis var. krusfaki (Btk) e de Baculovirus (Bv)). As micropartículas obtidas com alginato de cálcio formaram pós contendo sistemas matriciais capazes de microencapsular e reter microrganismos entomopatogênicos, inclusive após redispersão em água. Os processos de microencapsulação de desenvolvidos e avaliados demonstraram ser adequados para a manutenção da viabilidade de Btk e da integridade dos poliedros de Bv, assim como, a conservação da capacidade bioinseticida destes microrganismos. As micropartículas obtidas com as misturas poliméricas Caseína/HEC e Caseína/HPMC pelo processo de spray drying revelaram-se inadequadas na medida que se dissolviam rapidamente depois de dispersas em água e não poderiam assim proteger o material bioinseticida no meio ambiente.
The aim of this work was to develop powder formulations containing microparticles, to be used as multiparticulate delivery systems. Two different methods were investigated : 1) the preparation of microparticles by spray drying using casein, hydroxyethylcellulose (HEC) and hydroxypropilmetilcellulose (HPMC) and 2) the preparation of calcium alginate and chitosan-alginate microparticles by using an atomizer device. Different experimental procedures to prepare calcium alginate and chitosanalginate microparticles were evaluated and variables beHeved to be important for the membrane formation were examined. The mean particles diarneter ranged from 60 to 553 µm. When a comparison was made between the surface morphology of calcium alginate and alginate-chitosan microparticles, remarkable roughness and more porous structure was observed in the chitosan-alginate microparticles. Some properties of the microparticles depended on the method and the procedure conditions of forming the chitosan-alginate complex. Calcium alginate and chitosan-alginate microparticles containing two different bioinsecticides were also prepared: 1) a spore/δ-endotoxinscomplex of Bacillus thuringiensis var.kurstaki (Btk) and 2) polyhedra of Baculovirus anticarsia (Bv), a viral insecticide. The results shown that the encapsulation of suspensions of Btk containing spore/toxin complex or polyhedra of B. anticarcia in calcium alginate and chitosan-alginate microparticles did not decrease the larvicidal activity of these biopesticides against lepidopterous pests. The formulations developed in this study remained unchanged, did not swell, did not release the spores (Btk) or the polyhedra (Bv), when dispersed in water and could be useful to be applied by aqueous spray as bioinsecticides in agriculture. On the other hand, casein, HEC and HPMC microparticles prepared by spray drying were not suitable to encapsulate bioinsecticides because they dissolved fastly after dispersion in water.
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Books on the topic "Applied microbiology"

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Saxena, Sanjai. Applied Microbiology. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2259-0.

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Durieux, Alain, and Jean Paul Simon, eds. Applied Microbiology. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/0-306-46888-3.

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W, Doelle H., and Hedén Carl-Göran, eds. Applied microbiology. Dordrecht: D. Reidel, 1986.

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Advances in applied microbiology. Amsterdam: Academic Press, 2011.

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L, Neidleman Saul, and Laskin Allen I, eds. Advances in applied microbiology. San Diego: Academic Press, 1995.

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Applied food microbiology. Belmont, CA: Star Pub. Co., 1997.

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Laskin, Allen I. Advances in Applied Microbiology, 32. Burlington: Elsevier, 1987.

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Neidleman, Saul L. Advances in Applied Microbiology, 37. Burlington: Elsevier, 1992.

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Laskin, Allen I. Advances in Applied Microbiology, 33. Burlington: Elsevier, 1988.

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Allen I. Laskin (Series Editor), Sima Sariaslani (Series Editor), and Geoffrey M. Gadd (Series Editor), eds. Advances in Applied Microbiology, Volume 61 (Advances in Applied Microbiology) (Advances in Applied Microbiology). Academic Press, 2007.

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Book chapters on the topic "Applied microbiology"

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Mokbel, K. M. "Microbiology." In MCQs in Applied Basic Sciences, 98–107. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2998-5_5.

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Pot, Bruno, and Marjon Wolters. "11. Food microbiology." In Applied food science, 215–45. The Netherlands: Wageningen Academic Publishers, 2022. http://dx.doi.org/10.3920/978-90-8686-933-6_11.

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Vaskoska, Rozita. "12. Hostile microbiology." In Applied food science, 247–66. The Netherlands: Wageningen Academic Publishers, 2022. http://dx.doi.org/10.3920/978-90-8686-933-6_12.

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Rosenberg, Eugene. "Biotechnology and Applied Microbiology." In The Prokaryotes, 315–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-30194-0_13.

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Rosenberg, Eugene. "Biotechnology and Applied Microbiology." In The Prokaryotes, 284–98. New York, NY: Springer New York, 2006. http://dx.doi.org/10.1007/0-387-30741-9_12.

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Kim, Dong Sog, and Jung Ho Suh. "Comparison of PB2+ Removal Characteristics Between Biomaterials and Non-biomaterials." In Applied Microbiology, 177–83. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/0-306-46888-3_13.

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Saxena, Sanjai. "Diversity of Industrially Relevant Microbes." In Applied Microbiology, 1–11. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2259-0_1.

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Saxena, Sanjai. "Strategies of Strain Improvement of Industrial Microbes." In Applied Microbiology, 155–71. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2259-0_10.

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Saxena, Sanjai. "Vaccines and Their Production." In Applied Microbiology, 173–78. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2259-0_11.

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Saxena, Sanjai. "Immobilisation and Biosensors." In Applied Microbiology, 179–90. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2259-0_12.

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Conference papers on the topic "Applied microbiology"

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Viera, Javier Méndez, Joan C. Ferrer, and Josep M. Fernández-Novell. "How industrial microbiology could be used to teach biotechnology." In MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0081.

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Leroux, Denis F., Rony Midahuen, Guillaume Perrin, Jeremie Pescatore, and Pierre Imbaud. "Hyperspectral imaging applied to microbial categorization in an automated microbiology workflow." In European Conference on Biomedical Optics. Washington, D.C.: OSA, 2015. http://dx.doi.org/10.1364/ecbo.2015.953726.

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Leroux, Denis F., Rony Midahuen, Guillaume Perrin, Jeremie Pescatore, and Pierre Imbaud. "Hyperspectral imaging applied to microbial categorization in an automated microbiology workflow." In European Conferences on Biomedical Optics, edited by J. Quincy Brown and Volker Deckert. SPIE, 2015. http://dx.doi.org/10.1117/12.2184140.

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Zaitsev, Boris. "Atomic Force Microscopy as a Tool for Applied Virology and Microbiology." In SCANNING TUNNELING MICROSCOPY/SPECTROSCOPY AND RELATED TECHNIQUES: 12th International Conference STM'03. AIP, 2003. http://dx.doi.org/10.1063/1.1639726.

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Arévalo-Villena, M., N. Barrajón-Simancas, E. Cerdeño-Gómez, and A. Briones. "Microbiology stability of wine from Castilla la Mancha." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0084.

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Castillo Reyna, Josefina, Carmen Daniela Gonzalez Barriga, and Gerardo Amador Silveyra Sáenz. "THE EXPERIENTIAL LEARNING IN MICROBIOLOGY LABORATORY CLASS APPLIED IN SOCIAL ASSISTANCE FOUNDATIONS." In 14th International Conference on Education and New Learning Technologies. IATED, 2022. http://dx.doi.org/10.21125/edulearn.2022.2467.

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Fernández-Novell, J. M., D. Cifuentes, C. Madrid, and J. C. Ferrer. "A new strategy for introducing Secondary school students to Microbiology and Biotechnology." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0129.

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Antunes, Adriana Almeida, A. M. A. T. Jara, J. M. Luna, C. D. C. Albuquerque, A. S. Messias, C. A. Alves-Silva, and G. M. Campos-Takaki. "Factorial design applied to biosurfactant production by Chromobacterium violaceum." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0052.

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Montero, B., J. L. García-Morales, D. Sales, and R. Solera. "Comparative analysis of different microbial techniques of quantification applied to anaerobic digestion." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0026.

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Yakupoglu, Tugrul, Suheyda F. Hepsen, Nutullah Ozdemir, and Ridvan Kizilkaya. "The effects of various organic wastes applied into eroded soil on dehydrogenase enzyme activity." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0021.

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Reports on the topic "Applied microbiology"

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Author, Not Given. Applied and Environmental Microbiology [agenda and attendee list]. Office of Scientific and Technical Information (OSTI), July 1999. http://dx.doi.org/10.2172/806576.

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Drake, Harold. 2001 Gordon Research Conference on Applied and Environmental Microbiology. Final progress report [agenda and attendee list]. Office of Scientific and Technical Information (OSTI), July 2001. http://dx.doi.org/10.2172/806640.

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Weinberg, Zwi G., Richard E. Muck, Nathan Gollop, Gilad Ashbell, Paul J. Weimer, and Limin Kung, Jr. effect of lactic acid bacteria silage inoculants on the ruminal ecosystem, fiber digestibility and animal performance. United States Department of Agriculture, September 2003. http://dx.doi.org/10.32747/2003.7587222.bard.

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Abstract:
The overall objective of the whole research was to elucidate the mechanisms by which LAB silage inoculants enhance ruminant performance. The results generated will permit the development of better silage inoculants that maximize both silage preservation and animal performance. For this one-year BARD feasibility study, the objectives were to: 1. determine whether lactic acid bacteria (LAB) used in inoculants for silage can survive in rumen fluid (RF) 2.select the inoculants that survived best, and 3. test whether LAB silage inoculants produce bacteriocins-like substances. The most promising strains will be used in the next steps of the research. Silage inoculants containing LAB are used in order to improve forage preservation efficiency. In addition, silage inoculants enhance animal performance in many cases. This includes improvements in feed intake, liveweight gain and milk production in 25-40% of studies reviewed. The cause for the improvement in animal performance is not clear but appears to be other than direct effect of LAB inoculants on silage fermentation. Results from various studies suggest a possible probiotic effect. Our hypothesis is that specific LAB strains interact with rumen microorganisms which results in enhanced rumen functionality and animal performance. The first step of the research is to determine whether LAB of silage inoculants survive in RF. Silage inoculants (12 in the U.S. and 10 in Israel) were added to clarified and strained RF. Inoculation rate was 10 ⁶ (clarified RF), 10⁷ (strained RF) (in the U.S.) and 10⁷, 10⁸ CFU ml⁻¹ in Israel (strained RF). The inoculated RF was incubated for 72 and 96 h at 39°C, with and without 5 g 1⁻¹ glucose. Changes in pH, LAB numbers and fermentation products were monitored throughout the incubation period. The results indicated that LAB silage inoculants can survive in RF. The inoculants with the highest counts after 72 h incubation in rumen fluid were Lactobacillus plantarum MTD1 and a L. plantarum/P. cerevisiae mixture (USA) and Enterococcus faecium strains and Lactobacillus buchneri (Israel). Incubation of rumen fluid with silage LAB inoculants resulted in higher pH values in most cases as compared with that of un-inoculated controls. The magnitude of the effect varied among inoculants and typically was enhanced with the inoculants that survived best. This might suggest the mode of action of LAB silage inoculants in the rumen as higher pH enhances fibrolytic microorganisms in the rumen. Volatile fatty acid (VFA) concentrations in the inoculated RF tended to be lower than in the control RF after incubation. However, L. plalltarull1 MTDI resulted in the highest concentrations of VFA in the RF relative to other inoculants. The implication of this result is not as yet clear. In previous research by others, feeding silages which were inoculated with this strain consistently enhanced animal performance. These finding were recently published in Weinberg et.al.. (2003), J. of Applied Microbiology 94:1066-1071 and in Weinberg et al.. (2003), Applied Biochemistry and Biotechnology (accepted). In addition, some strains in our studies have shown bacteriocins like activity. These included Pediococcus pentosaceus, Enterococcus faecium and Lactobacillus plantarum Mill 1. These results will enable us to continue the research with the LAB strains that survived best in the rumen fluid and have the highest potential to affect the rumen environment.
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