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Dissertations / Theses on the topic 'Applied Microbiology and Biotechnology'

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Published: 9 July 2021

Last updated: 25 July 2025

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1

Ishii, Marina. "Determinação dos parâmetros cinéticos de resistência térmica da Proteína Verde Fluorescente recombinante (GFPuv)." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/9/9135/tde-25062012-114829/.

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Células transformadas de E.coli DH5-α expressando a proteína verde fluorescente (GFPuv, pico de excitação e emissão de 394nm e 509nm) foram submetidas a extração pelo método de partição de três fases (TPP) e o extrato obtido purificado por cromatografia de interação hidrofóbica (HIC). O objetivo principal deste trabalho foi estudar a termoestabilidade da GFPuv extraída, para avaliar a sua possível utilização como indicador biológico econômico, de resposta rápida e precisa para processos térmicos de esterilização utilizando o calor úmido. A estabilidade térmica da proteína foi estudada em

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2

Pereira, da Silva Beltrão Eudinice. "Estudo do impacto do óleo diesel em solo domanguezal de Vila Velha-Itamaracá." Universidade Federal de Pernambuco, 2005. https://repositorio.ufpe.br/handle/123456789/1569.

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Made available in DSpace on 2014-06-12T15:51:12Z (GMT). No. of bitstreams: 2 arquivo4477_1.pdf: 2619648 bytes, checksum: c371ffaaab9afa32bc9f96397ed74514 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2005<br>O presente estudo teve por objetivo analisar o impacto do óleo Diesel na microbiota nativa do solo de manguezal de Vila Velha- Itamaracá, analisando a capacidade biodegradadora natural dos microrganismos nativo presente nesta área. Os resultados mostraram que microrganismos solo de manguezal (fungos e bactérias) quando submetidos a i

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3

Jepras, Robert Ian. "Applications of photon correlation spectroscopy and flow cytometry to microbiology." Thesis, Open University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.290872.

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4

Dabdoub, Shareef Majed. "Applied Visual Analytics in Molecular, Cellular, and Microbiology." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1322602183.

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5

Viktor, Marko Johann. "The expression of fungal enzymes in Saccharomyces cerevisiae for bio-ethanol production from raw cornstarch." Thesis, Stellenbosch : Stellenbosch University, 2011. http://hdl.handle.net/10019.1/6880.

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Thesis (MSc (Microbiology))--Stellenbosch University, 2011.<br>ENGLISH ABSTRACT: Reliable energy resources could be considered as one of the cornerstones of the prosperity of the human race. The growing human population is constantly exerting more pressure on the world’s natural resources, which include natural fossil fuels that are non‐renewable. There are concerns regarding the use of fossil fuels due to its growing scarcity and its negative impact on the environment. There is thus a growing need in the world for energy sources that are renewable, more or less carbon neutral and therefo

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Sunderland, Naomi Louise. "Biotechnology as Media: A Critical Study of the Movement of Meanings Associated with Contemporary Biotechnology." Thesis, Queensland University of Technology, 2004. https://eprints.qut.edu.au/16705/1/Naomi_Sunderland_Thesis.pdf.

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This thesis purports to make two contributions to understandings of biotechnology. First, it presents a novel framework through which to view biotechnology as a complex series of fundamentally social and politically economic mediations rather than a decontextualised collection of technical and scientific phenomena. Second, the thesis presents a method for analysing contemporary discourses about biotechnology within this framework. The framework presented in the first content chapter of the thesis identifies what I see to be the four primary mediating "movements" that are central to seeing Biot

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Sunderland, Naomi Louise. "Biotechnology as Media: A Critical Study of the Movement of Meanings Associated with Contemporary Biotechnology." Queensland University of Technology, 2004. http://eprints.qut.edu.au/16705/.

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This thesis purports to make two contributions to understandings of biotechnology. First, it presents a novel framework through which to view biotechnology as a complex series of fundamentally social and politically economic mediations rather than a decontextualised collection of technical and scientific phenomena. Second, the thesis presents a method for analysing contemporary discourses about biotechnology within this framework. The framework presented in the first content chapter of the thesis identifies what I see to be the four primary mediating "movements" that are central to seeing Biot

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8

Iker, Brandon Charles. "Application of Advanced Molecular Techniques in Applied Environmental Microbiology." Diss., The University of Arizona, 2013. http://hdl.handle.net/10150/301699.

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Recent advancements in molecular biology such as next generation sequencing and more sensitive and rapid molecular detection methods like qPCR, have historically been developed for clinical applications in human genetics and for health care diagnostic purposes. The high demand for faster and more accurate molecular assays in the health care field has driven rapid development of inexpensive molecular techniques that when applied to the science of environmental microbiology, provides an unprecedented level of understanding of the microbial world around us. The goal of this dissertation is to be

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9

DOUGUE, KENTSOP ROMEO ARAGO. "Biotechnology applied to aromatic plants for the controlled production of bioactive compounds." Doctoral thesis, Università degli studi di Genova, 2020. http://hdl.handle.net/11567/1001566.

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Salvia sensu lato includes more than about 9000 species and is considered one of the largest taxa of the Lamiaceae (Will et al., 2015). For a long time, Salvia species were known for a wide variety of medicinal used in folk medicine for the relief of pain, protecting the body against oxidative stress, free radical damages, angiogenesis, inflammation, bacterial and virus infection, etc… (Hamidpour et al., 2014). In vitro cultures have been considered as an alternative agricultural processes for producing secondary metabolites (Y. Kim et al., 2002). The research activity of this PhD project was

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10

Jacobson, Daniel A. "Networks and multivariate statistics as applied to biological datasets and wine-related omics." Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/85630.

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Thesis (PhD)--Stellenbosch University, 2013.<br>ENGLISH ABSTRACT: Introduction: Wine production is a complex biotechnological process aiming at productively coordinating the interactions and outputs of several biological systems, including grapevine and many microorganisms such as wine yeast and wine bacteria. High-throughput data generating tools in the elds of genomics, transcriptomics, proteomics, metabolomics and microbiomics are being applied both locally and globally in order to better understand complex biological systems. As such, the datasets available for analysis and mining

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11

Kotze, Andries Albertus. "Analysis of arsenic resistance in the biomining bacterium, Acidithiobacillus caldus." Thesis, Stellenbosch : University of Stellenbosch, 2007. http://hdl.handle.net/10019.1/17374.

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Thesis (MSc)--University of Stellenbosch, 2006.<br>ENGLISH ABSTRACT: In this study the chromosomal arsenic resistance (ars) genes shown to be present in all Acidithiobacillus. caldus isolates were cloned and sequenced from At. caldus #6. Ten open reading frames (ORFs) were identified on a clone conferring arsenic resistance, with three homologs to arsenic genes, arsC (arsenate reductase), arsR (regulator) and arsB (arsenite export). This ars operon is divergent, with the arsRC and arsB genes transcribed in opposite directions. Analysis of the putative amino acid sequences of these arsRC and ar

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12

De, Villiers Gillian K. "Development of recombinant Saccharomyces cerevisiae for improved D-xylose utilisation." Thesis, Stellenbosch : University of Stellenbosch, 2006. http://hdl.handle.net/10019.1/17346.

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Thesis (MSc)--University of Stellenbosch, 2006.<br>ENGLISH ABSTRACT: Plant biomass is potentially an inexhaustible source of bioenergy. To be more useful in an industrialised context, conversion to liquid biofuel is necessary, which could provide the motor vehicle market with energy. To enable fermentation of both hexose and pentose sugars present in plant biomass, many researchers have introduced eukaryotic D-xylose utilisation metabolic pathways into S. cerevisiae as these yeasts cannot utilise D-xylose. The aim of this study was to increase D-xylose utilisation and lower the xylitol pr

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Laubscher, Inge. "Characterisation of plasmid p31T1 isolated from Aeromonas." Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/85725.

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Thesis (MSc)--Stellenbosch University, 2013.<br>ENGLISH ABSTRACT: Plasmids are an integral part of the horizontal gene pool and, therefore, are the main vectors for the spread of antibiotic and heavy metal resistance genes in the environment. Functional and taxonomic characterization of novel plasmids is, therefore, central to our general understanding of plasmid biology and their contribution to microbial evolution. Two 14-kb mobilizable plasmids, p31T1 and p36T2, conferring resistance to tetracycline were isolated from the opportunistic fish pathogens Aeromonas sobria and Aeromonas hyd

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Sinebe, Brekumoh Sarah. "Application of microbial biosensors for groundwater and wastewater monitoring." Thesis, University of Aberdeen, 2015. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=227614.

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15

Cambray, Garth Anton. "African mead biotechnology and indigenous knowledge systems in iQhilika process development." Thesis, Rhodes University, 2005. http://hdl.handle.net/10962/d1003988.

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While the production of mead, a fermented honey beverage, has declined in popularity around the world in recent centuries, a substantial mead industry continues to exist in Africa with an estimated annual production of 1 to 1.7 billion litres. This is largely an ‘invisible industry’, and has functioned outside the formal economy due to proscription of indigenous beverages during colonial times. The traditional African mead industry is, however, also now under pressure due to the environmental degradation of scarce natural ingredients, urbanisation and loss of indigenous knowledge systems (IKS)

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16

Lennox, Samuel David. "The applied mathematical modelling of milk and milk solids production." Thesis, Queen's University Belfast, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317476.

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17

Armstrong, Gareth Owen. "The production of resveratrol by wine yeast." Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52557.

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Thesis (MSc)--Stellenbosch University, 2001.<br>ENGLISH ABSTRACT: Grapevine is constantly under attack from a wide variety of pathogens including viruses, bacteria and fungi. In order to ensure survival, the grapevine has developed a vast array of defense mechanisms to combat invading organisms. A key element of this disease resistance is the production of phytoalexins, of which resveratrol is the primary component. The synthesis of resveratrol, together with other structural and biochemical defense mechanisms equips the plant to combat a number of pathogens resulting in the production

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De, Villiers Tania. "Fungal enzymes and microbial systems for industrial processing." Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/21457.

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Thesis (PhD)--Stellenbosch University, 2008.<br>ENGLISH ABSTRACT: This study strives to improve two current industrial processes by making them more cost effective through the use of hydrolytic enzymes or microbial systems. The first process targeted is the industrial conversion of starch to ethanol. In the second process, hydrolytic enzymes are applied to the manufacturing of instant coffee. The engineering of microbial systems to convert starch to bio-ethanol in a one-step process may result in large cost reductions in current industrial processes. These reductions will be due to decre

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Louw, Lilly-Ann. "Analysis of an 18kb accessory region of plasmid pTcM1 from Acidithiobacillus caldus MNG." Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/1667.

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Thesis (MSc (Microbiology))--University of Stellenbosch, 2009.<br>Biomining organisms are generally found in metal-rich, inorganic environments such as iron and sulfur containing ores; where they play a vital role in mineralization and decomposition of minerals. They are typically obligatory acidophilic, mesophilic or thermophilic, autotrophic, usually aerobic, iron-or sulfur oxidizing chemolithotrophic bacteria. The most prominent biomining organisms used in bioleaching of metal sulfides are Acidithiobacillus ferrooxidans, At. thiooxidans, At. caldus, Sulfobacillus spp. and Leptospirillu

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20

Serrat, Gurrera Xavier. "Applied biotechnology to improve Mediterranean rice varieties = Biotecnologia aplicada a la millora de varietats d’arròs mediterrànies." Doctoral thesis, Universitat de Barcelona, 2016. http://hdl.handle.net/10803/396188.

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The current world population is over 7.4 billion and expected to exceed 9 billion in 2040, causing a 70% increase in food demand. Global environmental degradation, in the form of salinization, pollution and global warming, has also reduced the availability of suitable arable land and water sources, contributing to promote crop improvement in order to increase the potential yields. Rice (Oryza sativa) is the most widely consumed staple food for a large proportion of the world population. Classical rice breeding programs use its natural variability to create new allelic combinations which are

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21

Goldschmidt, Gunther Karl. "Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f." Thesis, Stellenbosch : University of Stellenbosch, 2005. http://hdl.handle.net/10019.1/1588.

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Thesis (MSc (Microbiology))--University of Stellenbosch, 2005.<br>Plasmid pTC-F14 is a 14.2kb promiscuous, broad-host range IncQ-like mobilizable plasmid isolated from Acidithiobacillus caldus f. At. caldus is a member of a consortium of bacteria (along with Acidithiobacillus ferrooxidans and Leptospirilum ferrooxidans) that is used industrially for decomposing metal sulphide ores and concentrates at temperatures of 40ºC or below which is now a well-established industrial process to recover metals from certain copper, uranium and gold-bearing minerals or mineral concentrates. These biomining m

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Malactos, Michael D. "Investigations into the detection of injured Salmonella typhimurium in foodstuffs." Thesis, University of Bedfordshire, 1998. http://hdl.handle.net/10547/336486.

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A chemically defined medium for Salmonella growth was developed and optimised using supplements of amino acids, nucleosides, vitamins and different carbon sources. The medium developed was compared to commercially available pre-enrichment media BPW and Salmosyst. Growth of Salmonella was significantly higher in BPW and Salmosyst than the medium developed. The amino acid and nucleoside supplement was directly compared with peptone. The results show peptone to be nutritionally superior and promoting better growth of Salmonella. Three different ELISA assays were used to detect Salmonella growing

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23

Muhamad, Ali Howbeer. "Metabolomics investigation of microbial cell factories." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/metabolomics-investigation-of-microbial-cell-factories(2e2f5f58-d38a-4c77-966b-56ce92aec619).html.

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The stream of new technological advancements and their integration into the field of microbiology have contributed significantly towards our understanding of life in the micro-scale world, making the fields of microbiology and biotechnology shine like never before. Since 1980, the recombinant protein-based therapeutics industry has become one of the fastest growing sectors in the biopharmaceutical market. Nearly 30% of commercially available recombinant proteins are produced in Escherichia coli, making this species one of the most commonly used bacterial expression systems for the production o

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Coram, Nicolette Joanne. "Molecular characterization of iron-oxidizing Leptospirillum strains from around the world." Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/52944.

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Dissertation (PhD) -- University of Stellenbosch, 2002.<br>ENGLISH ABSTRACT: More than sixteen isolates of iron-oxidizing bacteria belonging to the genus Leptospirillum were included in this study, with the finding that they were clearly divisible into two major groups. Group I leptospirilla had mol% G+C ratios within the range 49-52%, three copies of rrn genes and based on 16S rRNA sequence data, clustered together with the Leptospirillum ferrooxidans type strain (DSM2705or LI5). Group II leptospirilla had mol% G+C ratios of 55-58%, two copies of rrn genes and based on 16S rRNA sequenc

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Igbinigie, Eric Egbe. "The rhizosphere as a bioprocess environment for the bioconversion of hard coal." Thesis, Rhodes University, 2008. http://hdl.handle.net/10962/d1003983.

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Fundamental processes involved in the microbial degradation of coal and its derivatives have been well investigated and documented over the past two decades. However, limited progress in industrial application has been identified as bottleneck in further active development of the field. The sporadic and unanticipated growth of Cynodon dactylon (Bermuda grass) has been observed on the surface of some coal dumps in the Witbank coal mining area of South Africa. Preliminary investigations showed the formation of a humic soil-like material from the breakdown of hard coal in the root zone of these p

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Leukes, W. "Development and characterisation of a membrane gradostat bioreactor for the bioremediation of aromatic pollutants using white rot fungi." Thesis, Rhodes University, 1999. http://hdl.handle.net/10962/d1004092.

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Bioremediation of aromatic pollutants using the ligninolytic enzymes of the white rot fungi has been thoroughly researched and has been shown to have considerable potential for industrial application. However, little success in scale-up and industrialisation of this technology has been attained due to problems associated with the continuous production of the pollutant-degrading enzymes using conventional bioreactor systems. The low productivities reported result from the incompatibility of conventional submerged culture reactor techniques with the physiological requirements of these fungi whic

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Booysen, Clifford. "Identification of bacteria isolated from malt, with the emphasis on lactic acid bacteria and their influence on brewer's yeast." Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52299.

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Thesis (MScAgric.)--University of Stellenbosch, 2001.<br>ENGLISH ABSTRACT: Changes in the bacterial population throughout the malting process of two barley cultivars, i.e. Clipper (local cultivar) and Prisma (imported cultivar), malted at Southern Associated Maltsters (SAM), Caledon, South Africa, were studied. Samples were taken from four individual runs of each cultivar at ten different stages, i.e. dry barley before steep, water from the first steep water-stand, barley after draining the first steep, water from the second steep water-stand, barley from the second steep water-stand, ba

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Schröder, Michael, Rainer Winnenburg, and Conrad Plake. "Improved mutation tagging with gene identifiers applied to membrane protein stability prediction." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-177379.

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Background The automated retrieval and integration of information about protein point mutations in combination with structure, domain and interaction data from literature and databases promises to be a valuable approach to study structure-function relationships in biomedical data sets. Results We developed a rule- and regular expression-based protein point mutation retrieval pipeline for PubMed abstracts, which shows an F-measure of 87% for the mutation retrieval task on a benchmark dataset. In order to link mutations to their proteins, we utilize a named entity recognition algorithm for the

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Van, Rooyen Ronel 1976. "Genetic engineering of the yeast Saccharomyces cerevisiae to ferment cellobiose." Thesis, Stellenbosch : Stellenbosch University, 2007. http://hdl.handle.net/10019.1/19455.

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Dissertation (PhD)--Stellenbosch University, 2007.<br>PCT patent registered: https://www.google.com/patents/WO2009034414A1?cl=en&dq=pct/ib2007/004098&hl=en&sa=X&ei=b7AxUsSZK4jB0gWi14HgCQ&ved=0CEkQ6AEwAg USA: https://www.google.com/patents/US20110129888?dq=pct/ib2007/004098&ei=b7AxUsSZK4jB0gWi14HgCQ&cl=en<br>USA patent registered: https://www.google.com/patents/US20110129888?dq=pct/ib2007/004098&ei=b7AxUsSZK4jB0gWi14HgCQ&cl=en<br>ENGLISH ABSTRACT: The conversion of cellulosic biomass into fuels and chemicals has the potential to positively impact the South African economy, but is reliant on

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Du, Plessis Heinrich Wilbur 1975. "The role of lactic acid bacteria in brandy production." Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/53161.

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Thesis (MSc)--University of Stellenbosch, 2002.<br>ENGLISH ABSTRACT: The presence and growth of lactic acid bacteria (LAB) in wine and their influence on wine quality has received much attention in recent years. Lactic acid bacteria are responsible for conducting malolactic fermentation (MLF) in wine. The benefits associated with malolactic fermentation in terms of deacidification of wine and the contribution to wine flavour and complexity have also recently been the topic of research. It is impossible to describe malolactic fermentation as distinctly desirable or undesirable in terms of

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Lunnbäck, Johan. "Hydrodynamic cavitation applied to anaerobic degradation of fats, oils and greases (FOGs)." Thesis, Linköpings universitet, Tema Miljöförändring, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-140685.

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To increase profitability for biogas production, new innovative substrates and condition of operations needs to be implemented. At the current state, fats, oils and greases (FOGs) represent a promising substrate even though it brings operational challenges to the anaerobic digestion process. By utilizing hydrodynamic cavitation (HC) as a pre-treatment of the FOGs, the efficiency of FOGs’ co-digestion with wastewater sludge can be significantly improved. Preliminary experiments conducted on oil and water demonstrates that the HC pre-treatment improves the oil solubilisation as well as forms sta

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Skipper, Philip. "Biodeterioration of limestone : role of bacterial biofilms and possible intervention strategies." Thesis, University of Lincoln, 2018. http://eprints.lincoln.ac.uk/33697/.

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Limestone built heritage is at risk from the effects of biofilms, a microbial community encapsulated in a matrix of sugars, protein and extracellular DNA. Although biofilm research has been carried out in Mediterranean regions, few studies cover temperate Northern Europe climates, or the UK. This study concentrates on bacterial colonisation of Lincoln limestone, a highly vulnerable building material, and identifies the species, their role in biodeterioration and the efficacy of biocides against them. As part of this study the core species which comprise the bacterial component of the limestone

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Bauer, Rolene. "Strategies for the control of malolactic fermentation : characterisation of Pediocin PD-1 and the gene for the malolactic enzyme from Pediococcus damnosus NCFB 1832." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/50106.

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Dissertation (PhD Agric)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Malolactic fermentation (MLF) is conducted by lactic acid bacteria (LAB) and entails the decarboxylation of L-malate to L-Iactate through a reaction catalysed by the malolactic enzyme (MLE). The consequence of this conversion is a decrease in total acidity. MLF plays a part in microbial stabilisation and due to the metabolic activity of the bacteria the organoleptic profile of the wine is modified. In some wines MLF is considered as spoilage, especially in warm viticultural regions with grapes containing le

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Strauss, Colin Earl, and University of Lethbridge Faculty of Arts and Science. "Development of Pichia pastoris as a ruminal escape vehicle." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2000, 2000. http://hdl.handle.net/10133/148.

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The yeast expression system Pichia pastoris was investigated as an encapsulation technology capable of serving as a rumen escape vehicle. Cellularly encapsulated protein is protected from the ruminal environment so long as the cell membrane, which surrounds and isolates the intracellular protein is physically intact. Intracellular expression of Green Fluorescent Protein (GFP) allows for the monitoring of cellular integrity as necessary for the protection of encapsulated protein from ruminal proteases. Upon cellular lysis GFP is exposed to extracellular proteases which result in both the proteo

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Volschenk, Heinrich. "Characterisation of L-malic acid metabolism in strains of Saccharomyces and the development of a commercial wine yeast strain with an efficient malo-ethanolic pathway." Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/52728.

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Dissertation (PhD)--University of Stellenbosch, 2002.<br>ENGLISH ABSTRACT: L-Malic and tartaric acid are the most prominent organic acids in wine and playa crucial role in winemaking processes and wine quality, including the organoleptic quality and the physical, biochemical and microbial stability of wine. The production of premium wines depends on the oenologist's skill to accurately adjust wine acidity to obtain the optimum balance with other wine components to produce wine with optimum colour and flavour. Strains of Saccharomyces, in general, rarely degrade L-malic acid completely in

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Fouche, Nicolette. "Expression of mannanases in fermentative yeasts." Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/4047.

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Thesis (MSc (Microbiology))--University of Stellenbosch, 2009.<br>ENGLISH ABSTRACT: The search for a cost-effective, environmentally friendly replacement for fossil fuels resulted in bio-ethanol production receiving a lot of attention. Lignocellulose, is considered to be the most abundant renewable source on earth, and consists of cellulose, hemicellulose and lignin. Exploitation thereof as a substrate for ethanol production, can serve as solution in producing bio-ethanol as an adequate replacement for fossil fuels. Hemicelluloses, contributing up to a third of the lignocellulosic substrate, c

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Ferreira, Jacques. "Factors influencing the fermentation performance of commercial wine yeasts." Thesis, Stellenbosch : University of Stellenbosch, 2004. http://hdl.handle.net/10019.1/16322.

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Thesis (MScAgric)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: The production of quality wine is influenced by numerous factors of which grape quality is one of the most important factors. The production of quality wine, however, is not possible without good winemaking techniques and effective quality control. Critical control points (CCP) during the winemaking process must be identified to ensure optimum wine quality. Grape must is a complex medium that contains different micro-organisms which can be either beneficial or negative to wine quality, depending on the physical and

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Rincon, Fontan Myriam. "Chiral amines by transaminase biocatalysis in organic media." Thesis, KTH, Skolan för bioteknologi (BIO), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-163686.

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Theron, Louwrens Wiid. "Expression and purification of recombinant extracellular proteases originating from non-Saccharomyces yeasts." Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/85704.

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Thesis (MSc)--Stellenbosch University, 2013.<br>ENGLISH ABSTRACT: During wine fermentation, yeasts release extracellular enzymes that significantly impact wine properties. While the extracellular proteins of Saccharomyces cerevisiae have been characterised, those of non-Saccharomyces yeasts remain largely unknown. Most of these enzymes break down sugar polymers or catalyse the liberation of glycosidically-bound molecules. Another category of enzymes of oenological interest is represented by acid proteases that are able to prevent or reduce protein haze, as reported in literature, while s

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Louw, Marli. "Metabolic, genetic and physiological responses to SO2 exposure and nutrient-limiting conditions in Brettanomyces bruxellensis." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86465.

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Thesis (MSc)--Stellenbosch University, 2014.<br>ENGLISH ABSTRACT: Brettanomyces bruxellensis has become of increasing interest over the past few decades yet this complex red wine spoilage yeast is still poorly understood and strain variance also leads to the contradictory results reported in literature. This yeast is responsible for the production of phenolic compounds, associated with off-flavours that render wine unpalatable. Sulphur dioxide (SO2) is the most commonly used antioxidant and antimicrobial preservative instrumental in the control of spoilage yeasts such as B. bruxellensis. Howev

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Oliveira, Aline Furtado 1989. "Desenvolvimento de sistema microfluídico baseado em gradiente de concentração difusivo para bioprocessos = Development of microfluidic system based on diffusive concentration gradient for bioprocess." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/266097.

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Orientadores: Lucimara Gaziola de La Torre, Reinaldo Gaspar Bastos<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Química<br>Made available in DSpace on 2018-08-25T04:50:36Z (GMT). No. of bitstreams: 1 Oliveira_AlineFurtado_M.pdf: 2165174 bytes, checksum: d3aa7acdee3edc7f222daf3f7a82f910 (MD5) Previous issue date: 2014<br>Resumo: A microfluídica é uma ciência que opera em pequenos volumes de fluídos dentro de canais em dimensões de micrômetros (10-6 m). Estes sistemas permitem controlar moléculas no espaço e no tempo, gerando resultados rápidos e confi

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Ferraz, Carlos Augusto Montenegro. "Otimização da esterilização industrial de artigos médico-hospitalares, por óxido de etileno, utilizando-se Bacillus subtilis como indicador biológico." Universidade de São Paulo, 1997. http://www.teses.usp.br/teses/disponiveis/9/9134/tde-24042015-112526/.

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O óxido de etileno é um agente esterilizante dos mais importantes para materiais termo-sensíveis, dentre eles os artigos médico-hospitalares. Fios para sutura cirúrgica foram submetidos à exposição, em autoclave, a misturas gasosas com capacidade anti-microbiana, monitorando-se o processo através de Bacillus subtilis como indicador biológico. O objetivo deste estudo foi determinar a relação da letalidade do ciclo em função dos parâmetros de processo temperatura, tempo e pressão, expressando-a através de equações polinomiais decorrentes de modelo de ajuste pelo método da regressão linear múltip

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Ngongang, Maxwell Mewa. "Production of biopreservation compounds from non-Saccharomyces yeast using a single-stage bioreactor." Thesis, Cape Peninsula University of Technology, 2016. http://hdl.handle.net/20.500.11838/2372.

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Thesis (MTech (Chemical Engineering))--Cape Peninsula University of Technology, 2016.<br>Microbial spoilage has been reported in various food products and this has led to increased food, fruit and beverage losses, thereby threatening economic growth, food safety and security. Furthermore, statistics have shown that more than 30% of agricultural produce in developing countries, mostly in Africa, is lost owing to microbial spoilage. Beverages, food and fruits are predominant contributors to the South African export market. In recent years, contamination of these products resulting in spoilage

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Knoll, Caroline. "Investigation of bacteriocins from lactic acid bacteria and their impact in winemaking." Thesis, Stellenbosch : University of Stellenbosch, 2007. http://hdl.handle.net/10019.1/2623.

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Thesis (MSc (Wine Biotechnology))--University of Stellenbosch, 2007.<br>Bacteriocins are ribosomally synthesized antimicrobial peptides produced by bacteria and are active against other bacteria, either in the same species (narrow spectrum) or across genera (broad spectrum). The application of bacteriocins during the vinification process might help to prevent the production of undesired compounds by inhibiting the indigenous bacterial microflora and allowing malolactic fermentation to be conducted by a selected bacterial strain. Furthermore, the use of bacteriocins might allow reducing the tot

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Downing, Lynn 1978. "Characterisation of biogenic amine genes in lactic acid bacteria isolated from wine." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53328.

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Thesis (MSc)--Stellenbosch University, 2003.<br>ENGLISH ABSTRACT: The winemaking process involves a complex microbial flora where the interaction of yeasts, lactic acid bacteria and acetic acid bacteria play an important role in the quality and wholesomeness of the final product. Yeasts are primarily responsible for alcoholic fermentation. Malolactic fermentation follows alcoholic fermentation and is conducted by lactic acid bacteria. These bacteria are important in winemaking and can have a positive or negative effect on the wine quality. Biogenic amines are one of the compounds produc

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Reid, Vernita Jennilee. "Extracellular acid proteases of wine microorganisms : gene identification, activity characterization and impact on wine." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/20322.

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Thesis (MSc)--Stellenbosch University, 2012.<br>ENGLISH ABSTRACT: Non-Saccharomyces yeasts of oenological origin have previously been associated with spoilage or regarded as undesired yeasts in wine. However, these yeasts have recently come under investigation for their positive contribution towards wine aroma especially when used in sequential or co-inoculated fermentations with Saccharomyces cerevisiae. These yeasts are also known to secrete a number of enzymes that could be applicable in wine biotechnology. Amongst these enzymes are aspartic proteases. The secreted proteases from some

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Ranwedzi, Ntanganedzeni. "Optimization of β-glucosidase activy in recombinant Saccharomyces cerevisiae strains". Thesis, Stellenbosch : Stellenbosch University, 2007. http://hdl.handle.net/10019.1/21731.

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Thesis (MSc)--University of Stellenbosch, 2007.<br>ENGLISH ABSTRACT: Wine is a complex medium. Wine aroma, flavour and colour are important quality factors, but these can be influenced by many factors, such as grape-derived compounds that exist as free volatiles and also as glycosidically bound. The chemical composition of wine is determined by factors such as grape variety, geographic position, viticulture condition, microbial ecology of the grape and the winemaking process. The varietals aroma is determined by both the volatile and the non-volatile compounds, such as monoterpenes, nori

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Magerman, Cynthia M. "The evaluation of Fourier transform infrared (FT-IR) spectroscopy for quantitative and qualitative monitoring of alcoholic wine fermentation." Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/2754.

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Thesis (MSc (Wine Biotechnology))--University of Stellenbosch, 2009.<br>ENGLISH ABSTRACT: Fermentation is a complex process in which raw materials are transformed into high-value products, in this case, grape juice into wine. In this modern and economically competitive society, it is increasingly important to consistently produce wine to definable specifications and styles. Process management throughout the production stage is therefore crucial to achieve effective control over the process and consistent wine quality. Problematic wine fermentations directly impact on cellar productivity a

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Ammar, Ehab Mohamed. "Metabolic Engineering of Propionibacteria for Enhanced Propionic Acid and n-Propanol Fermentative Production." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1374174278.

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Trollope, Kim. "Investigation of resveratrol production by genetically engineered Saccharomyces cerevisiae strains." Thesis, Stellenbosch : University of Stellenbosch, 2006. http://hdl.handle.net/10019.1/2230.

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Thesis (MSc (Wine Biotechnology))--University of Stellenbosch, 2006.<br>Resveratrol is a phytoalexin that is produced in the leaves and skins of grape berries in response to biotic and abiotic factors. Substitution and polymerisation of resveratrol units produce an array of compounds which form part of the active disease defence mechanism in grapevine. Wine is one of the major sources of resveratrol in the human diet. Resveratrol is one of the phenolic compounds present in wine that mediates protective effects on human health. It has been shown to prevent the development of cardiovascular dis

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