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1
Orlando, Uislei A., Carine M. Vier, Wayne R. Cast, Ning Lu, Ron A. Navales, and Steve S. Dritz. "170 Meta-analysis to Determine the Standardized Ileal Digestible Lysine Requirements of Growing-finishing Pigs from 11- to 150-kg." Journal of Animal Science 99, Supplement_1 (2021): 62–63. http://dx.doi.org/10.1093/jas/skab054.104.
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Abstract A meta-analysis was conducted to determine the standardized ileal digestible (SID) lysine (Lys) recommendations for 11–150 kg PIC pigs housed under commercial conditions. Data from 29 trials dating from 2013 to 2020 utilizing 48,338 pigs were recorded in a database. Number of pens/treatment and pigs/trial ranged from 9 to 16 and 12 to 25, respectively. Sire lines were PIC 337 in 25 trials, PIC 327 in 3 trials, and PIC TR4 and 327 in 1 trial. Dam lines were PIC Camborough in 18 trials and PIC Camborough 29 in 9 trials. The SID Lys to calorie ratio curves were built for both metabolizable energy (ME) and net energy (NE) systems using the feed ingredient composition in NRC (2012) for energy levels. The response variables average daily gain (ADG) and feed efficiency (G:F) were analyzed using generalized linear and non-linear mixed models with heterogeneous variance (Gonçalves et al., 2016). Each treatment mean within a trial (n = 288) was considered the experimental unit and each trial was used as a random effect. The models were developed for mixed gender pigs and the PIC 337 growth curves were used to estimate the recommendations for barrows and gilts. There was no evidence for an interaction between sire lines or dam lines and treatment (P > 0.10). The SID Lys to calorie recommendations are based on the average for ADG and G:F (Table 1). At these levels, approximately 100% of maximum ADG and 99.4% of maximum G:F are achieved. The NE to ME ratio that results in a similar SID Lys recommendation as a percentage of the diet ranged from approximately 0.72 to 0.74. The updated biological requirements for PIC pigs remained similar compared to previous PIC recommendations (Gonçalves et al., 2017). However, the requirement estimates have been adjusted for late nursery and late finishing phases.
2
Greider, C. W. "Telomerase is processive." Molecular and Cellular Biology 11, no. 9 (1991): 4572–80. http://dx.doi.org/10.1128/mcb.11.9.4572.
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Telomerase synthesizes tandem repeats of the sequence d(TTGGGG) onto input d(TTGGGG)n primer oligonucleotides (C. W. Greider and E. H. Blackburn, Cell 43:405-413). An intrinsic RNA component of the enzyme provides the template for d(TTGGGG)n repeat synthesis [C. W. Greider and E. H. Blackburn, Nature (London) 337:331-337, 1989; G.-L. Lu, J. D. Bradley, L. D. Attardi, and E. H. Blackburn, Nature (London) 344:126-132, 1990]. In a typical reaction, products greater than 2,000 nucleotides were synthesized in 60 min. Dilution and primer challenge experiments showed that these long products were synthesized processively. The apparent processivity was not due to a higher affinity of the enzyme for long d(TTGGGG) products over the shorter competitors. The degree of processivity was quantitated; telomerase synthesized approximately 520 nucleotides before half of the enzyme had dissociated. After dissociating, telomerase reinitiated d(TTGGGG)n synthesis on new primer oligonucleotides. The products from a telomerase reaction have a characteristic 6-nucleotide banding pattern (C. W. Greider and E. H. Blackburn, Cell 51:887-898, 1987). A strong pause in the reaction occurs after the addition of the first G in the sequence d(TTGGGG). Both the processivity and the banding pattern analysis imply that in the elongation mechanism there must be a translocation step after the 9 nucleotides of internal template RNA have been copied to the extreme 5' end.
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Greider, C. W. "Telomerase is processive." Molecular and Cellular Biology 11, no. 9 (1991): 4572–80. http://dx.doi.org/10.1128/mcb.11.9.4572-4580.1991.
Full textAbstract:
Telomerase synthesizes tandem repeats of the sequence d(TTGGGG) onto input d(TTGGGG)n primer oligonucleotides (C. W. Greider and E. H. Blackburn, Cell 43:405-413). An intrinsic RNA component of the enzyme provides the template for d(TTGGGG)n repeat synthesis [C. W. Greider and E. H. Blackburn, Nature (London) 337:331-337, 1989; G.-L. Lu, J. D. Bradley, L. D. Attardi, and E. H. Blackburn, Nature (London) 344:126-132, 1990]. In a typical reaction, products greater than 2,000 nucleotides were synthesized in 60 min. Dilution and primer challenge experiments showed that these long products were synthesized processively. The apparent processivity was not due to a higher affinity of the enzyme for long d(TTGGGG) products over the shorter competitors. The degree of processivity was quantitated; telomerase synthesized approximately 520 nucleotides before half of the enzyme had dissociated. After dissociating, telomerase reinitiated d(TTGGGG)n synthesis on new primer oligonucleotides. The products from a telomerase reaction have a characteristic 6-nucleotide banding pattern (C. W. Greider and E. H. Blackburn, Cell 51:887-898, 1987). A strong pause in the reaction occurs after the addition of the first G in the sequence d(TTGGGG). Both the processivity and the banding pattern analysis imply that in the elongation mechanism there must be a translocation step after the 9 nucleotides of internal template RNA have been copied to the extreme 5' end.
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Chećko, Jarosław, Tomasz Urych, Małgorzata Magdziarczyk, and Adam Smoliński. "Resource Assessment and Numerical Modeling of CBM Extraction in the Upper Silesian Coal Basin, Poland." Energies 13, no. 9 (2020): 2153. http://dx.doi.org/10.3390/en13092153.
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The paper presents the assessment of the resources of methane considered as the main mineral in the most prospective selected areas of the Upper Silesian Coal Basin, Poland in the region of undeveloped deposits. The methane resources were estimated by means of a volumetric method at three depth levels, 1000, 1250, and 1500 m. A part of the Studzienice deposit comprising three coal seams, 333, 336, and 337, located in a methane zone was chosen for the numerical modeling of simulated methane production. The presented static 3D model has been developed using Petrel Schlumberger software. The total resources of methane in the area amount to approximately 446.5 million of Nm3. Numerical simulations of methane production from the selected coal seams with hydraulic fracturing were conducted by means of Schlumberger ECLIPSE reservoir simulator. Based on the simulations, it was concluded that, in the first six months of the simulations, water is produced from the seams, which is connected with the decrease in the rock mass pressure. The process prompts methane desorption from the coal matrix, which in turn results in a total methane production of 76.2 million of Nm3 within the five-year period of the simulations, which constitutes about 17% of total methane resources (GIP). The paper also presents a detailed analysis of Polish legislation concerning the activities aimed at prospecting, exploring, and extracting the deposits of hydrocarbons.
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Sánchez, María Jesús Fernández, and María Teresa Becerra Traver. "ESTUDIO DE LAS ESTRATEGIAS DE ESCRITURA CIENTÍFICA UTILIZADAS POR INVESTIGADORES NOVELES." Cadernos de Educação Tecnologia e Sociedade 13, no. 3 (2020): 329. http://dx.doi.org/10.14571/brajets.v13.n3.329-337.
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The writing is an essential skill to share with the scientific community the findings obtained by the researchers; however, it requires the implementation of a series of strategies whose domain has not yet been acquired by researchers with less experience. This research has a double objective; on the one hand, it tries to know the opinion of the PhD students about their own process of scientific writing and, on the other, it analyzes the scientific texts produced by the novice researchers. For this, two instruments have been created: a brief questionnaire and a system of categories to analyze the 397 errors that have been detected in the scientific texts written by 11 PhD students from different areas chosen for convenience. The results show that approximately 40% of researchers with little experience previously plan the texts they write and, for the most part, use a scheme for this. In addition, more than 90% believe that the most complicated thing is to provide coherence and cohesion to the scientific text. In relation to errors, the most common occur in the introduction (43.6%) and the method (18.4%) of the text and are grammatical (39%) and APA regulations (24.9 %). Finally, some limitations of the study are discussed.
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Bawohl, Melanie, and Tom Nilges. "Electrochemical Properties of Ag5Te2Cl0.8Br0.2, a Representative of the Solid Solutions Ag5Te2Cl1–xBrx." Zeitschrift für Naturforschung B 63, no. 9 (2008): 1083–86. http://dx.doi.org/10.1515/znb-2008-0911.
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Impedance spectroscopic investigations of Ag5Te2Cl0.8Br0.2, a selected representative of the solid solutions Ag5Te2Cl1−xBrx with x = 0 - 1, proved the mixed-conducting property of this class of materials. Two polymorphs are realized in the temperature range of 309 to 410 K, the monoclinic β - phase (space group P21/n) and the tetragonal α-phase (space group I4/mcm) with an order-disorder phase transition temperature of 336 K (DSC). A sharp increase of the total and ionic conductivity can be observed at 337 K, in good accordance with the phase transition temperature. The electronic conductivity exceeds the ionic conductivity by approximately one order of magnitude over a wide range of temperature. Conductivities are σion = 6.9×10−5 (309 K) and σtot = 4.80×10−4 Ω−1 cm−1 (310 K) for β -Ag5Te2Cl0.8Br0.2 and σion = 1.6×10−2 (395 K) and σtot = 1.73×10−1 Ω−1 cm−1 (394 K) for α-Ag5Te2Cl0.8Br0.2.
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Igari, Atsuko, Takanori Moriki, Terumichi Nakagawa, Yusuke Yamaguchi, and Mitsuru Murata. "Functional Analysis of a Peptide Sequence (Thr323 - Cys337) In ADAMTS13 Disintegrin-Like Domain Revealed Two Discontinuous Sequences Involved In the Interaction with VWF." Blood 116, no. 21 (2010): 2200. http://dx.doi.org/10.1182/blood.v116.21.2200.2200.
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Abstract Abstract 2200 ADAMTS13 specifically cleaves multimeric von Willebrand factor (VWF) into smaller molecules to reduce its high reactivity with platelets. The disintegrin-like (D) domain, adjacent to the catalytic domain of ADAMTS13, plays an important role in the process of VWF cleavage. In this study, we aimed to elucidate critical peptide sequences in D-domain involved in the interaction with VWF. A series of partially overlapping peptide sequences, approximately 20 amino acids in length, covering the D-domain, were synthesized and the inhibitory effects on the catalytic activity of plasma ADAMTS13 was examined using FRETS-VWF73 assay. Consequently, some synthetic peptides were selected and the minimal length necessary for the inhibitory effect was determined as TFAREHLDMCQALSC (peptide323-337). Removal of the amino-terminal threonine diminished the inhibitory effect moderately, although deletion of the carboxyl-terminal cysteine abolished it completely. According to the amino acids alignment of ADAMTS family, this peptide sequence is not conserved, highlighting the specific role in the interaction with its substrate. From the recent analysis of crystal structure, amino-terminal half of the peptide323-337, TFAREHL (323-329), was disordered and designated as the variable (V) loop, which creates one of VWF-binding exosites (Akiyama, et al. Proc Natl Acad Sci USA. 2009; 106:19274-9). We hypothesized that the amino-terminal amino acids of the peptide323-337 contribute to VWF binding, whereas the carboxyl-terminal amino acids allow the structural stability of the peptide conformation. To evaluate the effect of carboxyl-terminal cysteine at 337, other synthetic peptides with alanine, serine, glycine or phenylalanine instead of the cysteine (C337A, C337S, C337G, or C337F) were tested about their inhibitory effects on the catalytic activity. Interestingly, C337A, C337S, C337G peptides exhibited slightly weaker inhibitory effects on VWF73 catalysis, although C337F peptide showed stronger inhibition than wild-type sequence, suggesting that the residue 337 regulates the characteristics of the peptide323-337. From the results of peptide screening, the amino- and carboxyl-terminal amino acids of the peptide323-337, TFAREHLDMCQALSC, likely play key roles in the inhibitory effects; therefore, the middle part of the sequence, HLDMC, was replaced by 5 alanines (AAAAA) or reversed sequence CMDLH. Surprisingly, the converted peptides still retained the equivalent level of inhibitory effects, indicating both sides of the amino- and carboxyl-terminal amino acids were especially significant in the interaction with VWF. In conclusion, we characterized the peptide sequence, TFAREHLDMCQALSC (323-337), in D-domain. The peptide clearly inhibited the cleavage of VWF73 and the both sides of amino- and carboxyl-terminal amino acids seemed especially important. The peptide sequence is supposed to bind to VWF for the precise cleavage in the process of proteolysis. By modifying this peptide sequence, such variant ADAMTS13 as gain-of-function recombinants might be developed, leading to an alternative anti-thrombotic drug. Disclosures: No relevant conflicts of interest to declare.
8
Palamarchuk, Olha, Roman Gurevych, Borys Maksymchuk, et al. "Studying Innovation as the Factor in Professional Self-Development of Specialists in Physical Education and Sport." Revista Romaneasca pentru Educatie Multidimensionala 12, no. 4 (2020): 118–36. http://dx.doi.org/10.18662/rrem/12.4/337.
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Lifetime employment of specialists in physical education and sport is possible only under the conditions of enhancing their professional self-development, whose essential component is innovative activity. The article aims to identify the current level of innovation of professional activities of specialists in physical education and sport. It is also essential to determine the main characteristics of the innovation indicator during professional self-development of specialists in physical education and sport. The selected psychodiagnostic methods have made it possible to diagnose specialists in physical education and sport and reveal the main characteristics of the innovation indicator, namely, average, quadratic deviation, asymmetry and excess. The paper also used following methodologies: self-efficacy diagnosis, the level of subjective control and the style of response to changes. The research sample comprised of specialists in physical education and sport of different age groups, with different experience and qualification degree. In total, the study involved 209 participants. The total dispersion of the selected factors for the research groups with a prevailing conservative style is approximately 61%. The development of capability for innovation in specialists in physical education and sport involves enhancing their psychological qualities such as particular sensitivity to professional changes, readiness to take risks and be responsible for possible failures, independent judgment while taking professional decisions, focus on personal and professional self-development, willingness to search for new professional ideas.
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Gause, I., and S. Edén. "Hormonal regulation of growth hormone binding and responsiveness in adipose tissue and adipocytes of hypophysectomized rats." Journal of Endocrinology 105, no. 3 (1985): 331–37. http://dx.doi.org/10.1677/joe.0.1050331.
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ABSTRACT The hormonal regulation of GH binding and the effects of GH on glucose metabolism were studied in hypophysectomized rats. Male rats (130–140 g) were hypophysectomized and on the day after the operation treatment with one or a combination of two hormones was started and continued for 7 days. The different hormonal treatments were (1) cortisone acetate, (2) insulin, (3) insulin plus cortisone acetate, (4) thyroxine, (5) thyroxine plus cortisone acetate and (6) GH. Glucose metabolism was studied by determining the production of CO2 from [14C]glucose in epididymal fat pads and adipocytes and the incorporation of [14C]glucose into lipids in adipocytes. Binding of GH was measured in cell aliquots using 125I-labelled human GH. In hypophysectomized control animals, GH binding was decreased to approximately 25% of the binding observed in adipocytes of normal rats. Insulin treatment increased GH binding by approximately 100% and the response to GH was markedly increased. Similar effects were achieved by thyroxine treatment. Basal levels of glucose oxidation were markedly decreased after hypophysectomy but were increased towards normal by insulin or thyroxine treatment. Neither cortisone nor GH treatment had any effect on GH binding or glucose metabolism. The results show that insulin and thyroxine may be important for the GH receptor and the insulin-like effect of GH in adipocytes. J. Endocr. (1985) 105, 331–337
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SAKA, MARIANA N., and JULIO A. LOMBARDI. "A new species of Goeppertia (Marantaceae) from the northeast Brazil and an identification key for species from the state of Sergipe." Phytotaxa 170, no. 1 (2014): 046. http://dx.doi.org/10.11646/phytotaxa.170.1.6.
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Marantaceae is a family with approximately 550 species distributed throughout the tropics, with the exception of Australia (Andersson 2003). Calathea Meyer (1818: 6) used to be the largest genus in the family, previously including ca. 300 species. After the molecular study on the genus by Borchsenius et al. (2012), which confirmed the genus as polyphyletic, Goeppertia Nees (1831: 337) was resurrected to include all the species (approximately 245) that were not in the former subgenus Calathea Körnicke (1862: 112), the Calathea lanicaulis group (Kennedy et al. 1988) and the synonymized genus Sanblasia Andersson (1984: 21). Goeppertia thus became the most representative genus of Marantaceae, in Brazil represented by ca. 100 species (Braga 2013). It is found chiefly in rainforest habitats at elevations, below 1500m (Kennedy et al. 1988). However, the number of species may yet be underestimated due to unknown diversity of Brazilian species occurring in drier areas.
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Hadidi, A., L. Giunchedi, A. M. Shamloul, C. Poggi-Pollini, and M. A. Amer. "Occurrence of Peach Latent Mosaic Viroid in Stone Fruits and Its Transmission with Contaminated Blades." Plant Disease 81, no. 2 (1997): 154–58. http://dx.doi.org/10.1094/pdis.1997.81.2.154.
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Peach latent mosaic viroid (PLMVd) is widely distributed (approximately 55%) in peach germplasm from Europe, Asia, North America, and South America. PLMVd, or a closely related viroid, was occasionally detected in cherry, plum, and apricot germplasm from countries in Europe or Asia. The cherry isolate of PLMVd is 337 nucleotides in length and is 91 to 92% homologous to PLMVd isolates from peach. Molecular hybridization experiments demonstrated that PLMVd is not related to the agent of peach mosaic disease. PLMVd was readily transmitted (50 to 70%) by contaminated blades to green shoots and lignified stems of peach GF-305 plants. These results indicate that the viroid may be transmitted in orchards with contaminated pruning equipment.
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de Bruyn Kops, Stephen M., and James J. Riley. "The effects of stable stratification on the decay of initially isotropic homogeneous turbulence." Journal of Fluid Mechanics 860 (December 11, 2018): 787–821. http://dx.doi.org/10.1017/jfm.2018.888.
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We report on direct numerical simulations of the decay of initially isotropic, homogeneous turbulence subject to the application of stable density stratification. Flows were simulated for three different initial Reynolds numbers, but for the same initial Froude number. We find that the flows pass through three different dynamical regimes as they decay, depending on the local values of the Froude number and activity parameter. These regimes are analogous to those seen in the experimental study of Spedding (J. Fluid Mech., vol. 337, 1997, pp. 283–301) for the wake of a sphere. The flows initially decay with little influence of stratification, up to approximately one buoyancy period, when the local Froude number has dropped below 1. At this point the flows have adjusted to the density stratification, and, if the activity parameter is large enough, begin to decay at a slower rate and spread horizontally at a faster rate, consistent with the predictions of Davidson (J. Fluid Mech., vol. 663, 2010, pp. 268–292) and the scaling arguments of Billant & Chomaz (Phys. Fluids, vol. 13, 2001, pp. 1645–1651). We refer to this second regime as the stratified turbulence regime. As the flows continue to decay, ultimately the activity parameter drops below approximately 1 as viscous effects begin to dominate. In this regime, the flows have become quasi-horizontal, and approximately obey the scaling arguments of Godoy-Diana et al. (J. Fluid Mech., vol. 504, 2004, pp. 229–238).
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Evans, C. D., B. Reynolds, C. Hinton, et al. "Effects of decreasing acid deposition and climate change on acid extremes in an upland stream." Hydrology and Earth System Sciences 12, no. 2 (2008): 337–51. http://dx.doi.org/10.5194/hess-12-337-2008.
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Abstract. This study assesses the major chemical processes leading to acid extremes in a small, moorland stream in mid-Wales, UK, which has been monitored since 1979. Results suggest that base cation (mainly calcium) dilution, the "sea-salt effect", and elevated nitrate pulses, are the major causes of seasonal/episodic minima in acid neutralising capacity (ANC), and that the relative importance of these drivers has remained approximately constant during 25 years of decreasing acid deposition and associated long-term chemical recovery. Many of the chemical variations causing short-term reductions in stream acidity, particularly base cation dilution and organic acid increases, are closely related to changes in water-flowpath and therefore to stream discharge. Changes in the observed pH-discharge relationship over time indicate that high-flow pH has increased more rapidly than mean-flow pH, and therefore that episodes have decreased in magnitude since 1980. However a two-box application of the dynamic model MAGIC, whilst reproducing this trend, suggests that it will not persist in the long term, with mean ANC continuing to increase until 2100, but the ANC of the upper soil (the source of relatively acid water during high-flow episodes) stabilising close to zero beyond 2030. With climate change predicted to lead to an increase in maximum flows in the latter half of the century, high-flow related acid episodes may actually become more rather than less severe in the long term, although the model suggests that this effect may be small. Two other predicted climatic changes could also detrimentally impact on acid episodes: increased severity of winter "sea-salt" episodes due to higher wind speeds during winter storms; and larger sulphate pulses due to oxidation of reduced sulphur held in organic soils, during more extreme summer droughts. At the Gwy, the near-coastal location and relatively small extent of peat soils suggest that sea-salt episodes may have the greatest influence.
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Kocman, Vladimir, та Jan T. Szymański. "The crystal structure and chemistry of the "red salt" α-trisilver-amidoselenate, Ag3NSeO3". Canadian Journal of Chemistry 68, № 9 (1990): 1606–10. http://dx.doi.org/10.1139/v90-247.
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The crystal structure of the α-modification of the "red salt" of trisilver amidoselenate, Ag3NSeO3 has been determined from MoKα X-ray diffraction data, and refined to R = 3.19% from 337 unique absorption-corrected observed reflections. The structure is rhombohedral, R3c (No. 161), with (hexagonal) a = 8.462(4), c = 11.372(6) Å. The formula unit has point symmetry 3 (C3), with Se and N on the threefold axis, each approximately tetrahedrally coordinated; Se by three oxygen atoms (at 1.651(8) Å and N (at 1.70(2) Å, and N by Se and three silver atoms (at 2.164(8) Å. The bond of 1.70(2) Å between Se and N is particularly short. The Ag3NSeO3 structural unit bridges to three adjacent units via short Ag … O contacts of 2.23(1) Å to form a three-dimensional network. Keywords: trisilver amidoselenate, selenium–nitrogen bond, X-ray structure.
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Smith, Harry A., Aaron Hengist, Joel Thomas, et al. "Glucose control upon waking is unaffected by hourly sleep fragmentation during the night, but is impaired by morning caffeinated coffee." British Journal of Nutrition 124, no. 10 (2020): 1114–20. http://dx.doi.org/10.1017/s0007114520001865.
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AbstractMorning coffee is a common remedy following disrupted sleep, yet each factor can independently impair glucose tolerance and insulin sensitivity in healthy adults. Remarkably, the combined effects of sleep fragmentation and coffee on glucose control upon waking per se have never been investigated. In a randomised crossover design, twenty-nine adults (mean age: 21 (sd 1) years, BMI: 24·4 (sd 3·3) kg/m2) underwent three oral glucose tolerance tests (OGTT). One following a habitual night of sleep (Control; in bed, lights-off trying to sleep approximately 23.00–07.00 hours), the others following a night of sleep fragmentation (as Control but waking hourly for 5 min), with and without morning coffee approximately 1 h after waking (approximately 300 mg caffeine as black coffee 30 min prior to OGTT). Individualised peak plasma glucose and insulin concentrations were unaffected by sleep quality but were higher following coffee consumption (mean (normalised CI) for Control, Fragmented and Fragmented + Coffee, respectively; glucose: 8·20 (normalised CI 7·93, 8·47) mmol/l v. 8·23 (normalised CI 7·96, 8·50) mmol/l v. 8·96 (normalised CI 8·70, 9·22) mmol/l; insulin: 265 (normalised CI 247, 283) pmol/l; and 235 (normalised CI 218, 253) pmol/l; and 310 (normalised CI 284, 337) pmol/l). Likewise, incremental AUC for plasma glucose was higher in the Fragmented + Coffee trial compared with Fragmented. Whilst sleep fragmentation did not alter glycaemic or insulinaemic responses to morning glucose ingestion, if a strong caffeinated coffee is consumed, then a reduction in glucose tolerance can be expected.
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Akagi, Cynthia G., Walter R. Schumm, and M. Betsy Bergen. "Dimensionality of the Kansas Family Strengths Scale and the Kansas Marital Satisfaction Scale as Revised to Capture Changes in Marital Satisfaction." Psychological Reports 93, no. 3_suppl (2003): 1267–74. http://dx.doi.org/10.2466/pr0.2003.93.3f.1267.
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The Kansas Marital Satisfaction Scale was modified to reflect issues of relationship development and administered, along with 19 items from a previously reported Kansas Family Strengths Scale by Schumm, Bollman, Jurich, and Hatch, to 337 married couples from a midwestern urban community. High internal consistency was obtained for the revised version (α = .93), and the dimensionality of the Kansas Family Strengths Scale was approximately similar to that reported previously. In both this and the previous study, communication and conflict resolution items factored together while items for enjoying time together and actually spending time together factored separately. Likewise, in both studies, normative and affective commitment factored separately, and the internal consistency of the affective commitment subscale exceeded that for the normative commitment subscale. While the subscales for affective commitment, communication or conflict resolution, positive interaction, and time together yielded fair to adequate internal consistency in both studies, both studies indicated very low internal consistency for the normative commitment subscale, suggesting a need for improvement in that particular subscale.
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Olubajo, F. O., and V. A. Oyenuga. "The Yield, Intake and Animal Production of Four Tropical Grass Species Grown at Ibadan." Nigerian Journal of Animal Production 1, no. 2 (2021): 217–24. http://dx.doi.org/10.51791/njap.v1i2.1972.
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The yield, intake and animal production of four grass species namely: Cynodon nlemfuensis var. robustus, Pennisetum purpureum Schum; Cynodon nlemfuensis var nlemfuensis (Ib8) and Panicum Maximum Jacq., designated as treatments M, N, P, and respectively, were studied over a period of 1,226 days. Each treatment was grazed at three stages of growth - at four, six and eight week intervals. Total dry matter yields for the experimental period ranged from approximately 22 tons for treatment N grazed at intervals of four weeks to 65 tons for treatment Q grazed at eight week intervals. The mean daily dry matter yield varied between approximately 18 kg for treatment N to 53 kg for treatment Q grazed at four and eight week intervals respectively. With the exception of treatment N, dry matter intake by sheep per kg of metabolic size per day decreased with increased maturity and ranged from 43 kg in treatment N to 86 kg in treatment P grazed every four weeks. Treatment means of liveweight increases of grazing White Fulani (Zebu) steers were 392 g, 360 g, 337 g and 226 g per head per day for treatments Q, N,P and M respectively. Though the protein content of the pastures was high enough even in the dry season to maintain the grazing animals, dry matter production and intake were usually very low.
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Pleško, I. Mavric, M. Viršcek Marn, Z. Miladinovic, and J. Zindovic. "First Report of Peach latent mosaic viroid in Peach Trees in Montenegro." Plant Disease 96, no. 1 (2012): 150. http://dx.doi.org/10.1094/pdis-06-11-0487.
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Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) are known to infect stone fruit species worldwide. The viroid infection can be latent or induce a variety of disease symptoms. Stone fruit samples were collected in Montenegro for a Plum pox virus (PPV) survey in 2007. Thirteen samples infected with PPV, taken from 12-year-old peach trees (Prunus persica (L.) Batsch, cv. Elegant Lady) in the area of Cemovsko field, were tested for the presence of PLMVd and HSVd by reverse transcription (RT)-PCR. Mild or severe mosaic, chlorotic rings, and fruit deformations were observed on some trees. Total RNA was extracted from all samples with a RNeasy Plant Mini Kit (Qiagen, Chatsworth, CA) and RT-PCR was performed. Samples were tested for HSVd and PLMVd infection using primer pairs RF-43/RF-44 for PLMVd (1) and VP-19/VP-20 for HSVd (2). Amplification products of approximately 348 bp were obtained from nine samples with PLMVd primers. Amplification products from seven samples were successfully cloned into pGEM-T Easy Vector (Promega, Madison, WI) and used for transformation of Escherichia coli. At least four clones of each sample were sequenced. Obtained sequences were 337 and 338 nucleotides long and shared 90.3 to 100% identity. Consensus sequences of each sample were deposited in GenBank under Accession Nos. JF927892–JF927898. They showed 92.6 to 97.9% identity among each other, 94 to 98% identity with the PLMVd isolate G sequence (Accession No. EF591868) and 91.8 to 94.4% identity with PLMVd sequence M83545. HSVd was not detected in analyzed samples. PLMVd infections were found on peach trees in an area where approximately 40% of the peach production is located. Therefore, PLMVd infections can pose a threat to peach production in Montenegro. To our knowledge this is the first report of PLMVd infection of peach in Montenegro. References: (1) S. Ambrós et al. J. Virol. 72:7397, 1998. (2) S. A. Kofalvi et al. J. Gen. Virol. 78:3177, 1997.
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Blum, Melissa A., Tivadar Orban, Daniel O. Beck, and Michael Kalafatis. "The Specific Contribution of Amino Acids 334 and 335 from Factor Va Heavy Chain to the Catalytic Efficiency of Prothrombinase." Blood 106, no. 11 (2005): 1027. http://dx.doi.org/10.1182/blood.v106.11.1027.1027.
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Abstract The prothrombinase complex, composed of the enzyme factor Xa, the cofactor factor Va, and the substrate prothrombin associated on a cell surface in the presence of divalent metal ions, catalyzes the activation of prothrombin to thrombin 300,000-fold more effectively than the enzyme, factor Xa, alone. We have demonstrated that amino acids E323, Y324 and E330, V331 are binding sites for factor Xa on the factor Va heavy chain and are required for coordinating the spatial arrangement of enzyme and substrate directing prothrombin cleavage at two spatially distinct sites. We have also demonstrated that amino acid region 332–336 contains residues that are involved in cofactor function. Peptide studies have identified amino acid residues 334DY335 as major participants in factor Va cofactor activity. We have employed site-directed mutagenesis to study the effect of these amino acids on the catalytic efficiency of prothrombinase. Recombinant factor V molecules with the mutations D334K and Y335F, designated factor VKF, and D334A and Y335A, designated factor VAA were produced, transiently transfected, expressed in COS7L cells, and purified. Kinetic studies demonstrate that while factor VaKF has a KD for factor Xa similar to the KD observed for wild type factor Va, the kcat of prothrombinase assembled with factor VaKF has approximately a 1.5-fold decreased value compared to kcat of prothrombinase assembled with the wild type cofactor molecule. On the contrary, prothrombinase assembled with factor VaAA was found to have a nearly 10-fold decrease kcat, compared to prothrombinase assembled with wild type factor Va. This data suggest that not all amino acid substitutions are well tolerated at positions 334–335. Analysis of the sequence 323–340 using the recently published completed model of coagulation factor Va (pdb entry 1Y61) revealed that amino acids 334–335 are located at the end of a beta-sheet. To ascertain the importance of these mutants and their contribution to cofactor activity we have combined the mutations of amino acids 334–335 with mutations at amino acids 323–324 (E323F, Y324F) and 330–331 (E330M, V331I). We thus created quadruple mutants resulting in recombinant factor VFF/KF, factor VFF/AA, factor VMI/KF and factor VMI/AA. These molecules were transiently expressed in COS-7L cells and studied for their ability to be incorporated into prothrombinase. Free energies associated with the catalytic efficiencies of prothrombinase assembled with each mutant were also calculated (ΔΔGint). The ΔΔGint of interaction for the double mutants, factor VaFF/KF and factor VaMI/KF, had positive values indicating that the side chains of amino acids 330EV331, 323EY324 and 334DY335 located in and around the factor Xa binding site interact in a synergistic manner resulting in the destabilization of the transition state complex and a decelerated rate of catalysis. Conversely, combining the factor Xa binding site mutants with recombinant factor VaAA result in ΔΔGint values of approximately zero. In conclusion, the data demonstrate that replacement of amino acids 334–335 by two hydrophilic residues results in decreased cofactor function. In contrast, replacement of these amino acids by two small hydrophobic residues do not appear to be well tolerated by the cofactor resulting in severely impaired cofactor activity. Altogether, these data demonstrate the importance of amino acid residues D334 and Y335 for the rearrangement of enzyme and substrate required for efficient catalysis.
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Endress, Anton G., Michael R. Jeffords, Laurie J. Case, and Lane M. Smith. "Ozone-Induced Acceptability of Yellow-Poplar and Black Cherry to Gypsy Moth Larvae." Journal of Environmental Horticulture 9, no. 4 (1991): 221–25. http://dx.doi.org/10.24266/0738-2898-9.4.221.
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Abstract The feeding behavior of 3rd instar gypsy moth (Lymantria dispar L. [Lepidoptera: Lymantriidae]) was examined on foliage from black cherry (Prunus seratina L.) and yellow-poplar (Liriadendran tulipifera L.) seedlings exposed to 71 ± 31, 212 ± 37, and 337 ± 31 μg m−3 ozone (O3) for 70 hours to gauge the effect of O3 stress on host acceptability. Normally, black cherry is a suboptimal food source and yellow-poplar is unacceptable. With feeding preference assays conducted in the laboratory using feeding arenas, the leaf area consumption of black cherry control foliage (exposed to ambient air containing 71 μg m−3 O3) by starved larvae was approximately twice that of yellow-poplar control foliage during the first 4 hours. By 8 hours, the leaf area consumed was the same for both species. O3-treated leaves of both species were preferred by the larvae relative to leaves exposed to ambient concentrations. The effect was pronounced for yellow-poplar, where consumption of ozonated foliage was more than twice that of the control, and its acceptability was enhanced to a level approximating that of black cherry.
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Ananthanarayanan, M., O. C. Ng, J. L. Boyer, and F. J. Suchy. "Characterization of cloned rat liver Na(+)-bile acid cotransporter using peptide and fusion protein antibodies." American Journal of Physiology-Gastrointestinal and Liver Physiology 267, no. 4 (1994): G637—G643. http://dx.doi.org/10.1152/ajpgi.1994.267.4.g637.
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A cDNA encoding a rat liver Na(+)-bile acid cotransporter (Ntcp) has recently been cloned (Hagenbuch, B., B. Steiger, M. Fouget, H. Lubbert, and P. J. Meier. Proc. Natl. Acad. Sci. USA 88: 10629, 1991) using expression cloning in Xenopus laevis oocytes. Although the open reading frame coded for a protein of 39 kDa, in vitro translation experiments produced a 35-kDa protein which increased to a product of 41 kDa after glycosylation by pancreatic microsomes. To more clearly characterize the native protein in rat liver, we have raised antipeptide and anti-fusion protein antibodies to the COOH-terminal part of the cloned transporter. On Western blot analysis both antisera but not preimmune serum specifically detected a protein of approximately 50 kDa in isolated rat liver basolateral plasma membranes (BLPM). The reactivity was abolished when the antiserum was preincubated with the synthetic alpha-337 peptide. Deglycosylation of BLPM with N-glycanase followed by antibody probing led to decrease of the molecular mass to 34.5 kDa, suggesting that the protein is N-glycosylated in vivo. Two-dimensional immunoblotting indicated that the Ntcp protein had an isoelectric point of approximately 6.0. The antibody did not react with any proteins in rat ileal and kidney cortex brush-border membranes, human liver basolateral plasma membranes, or rat hepatoma tissue culture cell homogenates. Immunofluorescence localization studies with both antibodies revealed specific staining of the sinusoidal membrane domain but not of intracellular or bile canalicular membranes. Moreover, there was no acinar gradient in the pattern of staining.(ABSTRACT TRUNCATED AT 250 WORDS)
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Seymour, R. J., and B. K. Garside. "Ultrafast silicon interdigital photodiodes for ultraviolet applications." Canadian Journal of Physics 63, no. 6 (1985): 707–11. http://dx.doi.org/10.1139/p85-110.
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Conventional ultrafast photodetectors in use today generally have very small quantum efficiencies in the ultraviolet where the absorption length is very short. To extend the spectral range further into the ultraviolet, a strong electric field is required right at the surface on which the photons are incident. One method of achieving the required field is the use of two sets of interlocked metal digits, forming two back-to-back Schottky barriers on the surface of the semiconductor. Since both contacts are on the top surface, a thin semiconducting film on an insulating substrate can be used, greatly reducing depletion capacitance.We report a preliminary investigation of the potential of such devices using silicon on sapphire as an examplar material. Results are presented that show that capacitances of approximately 1 pF were obtained for devices with an area in the order of 10−3 cm2. These devices had a measured response time of less than 100 ps and measured quantum efficiencies at 337 run in the order of 10%, without the benefit of an antireflective coating.These preliminary results show that this type of device has excellent potential as an ultrafast, ultraviolet photodetector.
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Weger, J., P. Hutla, and J. Bubeník. "Yield and fuel characteristics of willows tested for biomass production on agricultural soil." Research in Agricultural Engineering 62, No. 4 (2016): 155–61. http://dx.doi.org/10.17221/12/2014-rae.
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Short rotation coppice (SRC) plantations of fast growing trees in Czech Republic may be grown on approximately 120,000 ha in future, as indicated in the Czech Action Plan for Biomass. Until now, 2,800 ha of SRC were established using predominantly poplar non-native hybrid clone Max-4. The aim of this experiment and article is evaluation of promising domestic clones of willow species and their natural hybrids, to discover their yield parameters and fuel characteristics in field conditions. The experiment was established in Průhonice in 2005 and it was harvested two times in triennial rotation (2008, 2011). For the test of fuel properties biomass was used from three willow species: common osier (Salix viminalis) S-337, Smith’s willow (S.× smithiana) S-218 and white willow (S. alba). Mean hectare yield of biomass in oven dry tonnes (o.d.t) of all the observed clones after first harvest was 3.9 o.d.t/ha/year, and after second harvest 10.4 o.d.t/ha/year. Observed willow clones had comparable or better yields as well as selected fuel properties (content of ash, melting point) in comparison with poplar clones used in the experiment.
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Rondanelli, Mariangela, Gabriella Peroni, Federica Fossari, et al. "Evidence of a Positive Link between Consumption and Supplementation of Ascorbic Acid and Bone Mineral Density." Nutrients 13, no. 3 (2021): 1012. http://dx.doi.org/10.3390/nu13031012.
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In animal models it has been shown that ascorbic acid (AA) is an essential cofactor for the hydroxylation of proline in collagen synthesis. However, there are still no precise indications regarding the role of AA in maintaining bone health in humans, so the aim of this narrative review was to consider state of the art on correlation between bone mineral density (BMD), AA dietary intake and AA blood levels, and on the effectiveness of AA supplement in humans. This review included 25 eligible studies. Fifteen studies evaluated correlations between AA intake and BMD: eight studies demonstrated a positive correlation between AA dietary intake and BMD in 9664 menopausal women and one significant interaction between effects of AA intake and hormone therapy. These data were also confirmed starting from adolescence (14,566 subjects). Considering studies on AA blood concentration and BMD, there are four (337 patients) that confirm a positive correlation. Regarding studies on supplementation, there were six (2671 subjects), of which one was carried out with AA supplementation exclusively in 994 postmenopausal women with a daily average dose of 745 mg (average period: 12.4 years). BMD values were found to be approximately 3% higher in women who took supplements.
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Comer, M. B., D. L. Campbell, R. L. Rasmusson, et al. "Cloning and characterization of an Ito-like potassium channel from ferret ventricle." American Journal of Physiology-Heart and Circulatory Physiology 267, no. 4 (1994): H1383—H1395. http://dx.doi.org/10.1152/ajpheart.1994.267.4.h1383.
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FK1, a ferret ventricular full-length cDNA clone, encodes a 654-amino acid protein with 98% identity to human K+ transient outward current (Ito)-like HK1 (Tamkun et al. FASEB J.5: 331-337, 1991). FK1 is detectable in ferret brain, atrium, left and right ventricle, and kidney but not in skeletal muscle, endothelial cells, aorta, and liver. In Xenopus oocytes, FK1 cRNA gives rise to a rapidly activating and inactivating Ito-like current, which is highly K+ selective (Na(+)-to-K+ permeability ratio = 0.003). Activation occurs over an approximately 50-mV range (-40 to +10 mV) and displays a sigmoid delay in onset with potential-dependent time constants that decrease with depolarization. Steady-state activation can be described with either a simple Boltzmann relationship [half-activation potential (V1/2) = -25 mV, slope (k) = 10 mV] or a Boltzmann relationship raised to either the third or fourth power (alpha 3: V1/2 = -43 mV, kappa = 13.1 mV; alpha 4: V1/2 = -48 mV, kappa = 13.6 mV, where alpha is the activation variable). Inactivation kinetics are biexponential, with the main fast time constant becoming independent of membrane potential depolarized to 0 mV. Steady-state inactivation can be described with a single Boltzmann relationship (V1/2 = -57 mV, kappa = 5.0 mV). Fast inactivation is removed by NH2-terminal deletions. Recovery from inactivation (-90 mV) is quite slow (half-time = 4.8 +/- 2.5 s). In 2 mM extracellular K+ concentration ([K+]o), FK1 tail currents display conventional deactivation behavior; however, in 98 mM [K+]o the tail currents display "reopening" behavior. These results suggest a molecular basis for the electrophysiological similarities between ferret and human ventricular Ito (Campbell et al. J. Gen. Physiol. 101: 571-601, 1993; Nabauer et al. Circ. Res. 73: 386-394, 1993).
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Xie, Ping, and Jiankang Liu. "Practical Success of Biomanipulation using Filter-Feeding Fish to Control Cyanobacteria Blooms: A Synthesis of Decades of Research and Application in a Subtropical Hypereutrophic Lake." Scientific World JOURNAL 1 (2001): 337–56. http://dx.doi.org/10.1100/tsw.2001.67.
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337 Lake Donghu is a 32-km 2 shallow, subtropical lake near the Yangtze River (P.R. China) that has experienced dramatic changes in the past five decades. These changes include: (1) a trophic state change from mesotrophy to hypertrophy; (2) dense blooms of cyanobacteria during every summer from the 1970s to 1984; (3) a cessation of blooms starting in 1985, with no recurrence; and (4) an increase, coincident with bloom declines, in the production of silver and bighead carp (filter-feeders) by more than tenfold. There are several possible explanations for the disappearance of blooms, including changes in nutrient concentrations, increased zooplankton grazing, and increased grazing on algae by fish. The long-term data suggest that changes in nutrients or in zooplankton were not important, but that the remarkably increased fish densities might have played the key role. To test this hypothesis, in situ enclosure experiments were conducted in three years. The main conclusions are as follows: (1) an increased stocking of the lake with carp played a decisive role in the elimination of cyanobacteria blooms; (2) both silver and bighead carp can eliminate cyanobacteria blooms directly by grazing; (3) zooplankton cannot suppress the blooms; and (4) the lake still is vulnerable to the outbreak of blooms, should fish grazing decline. The critical biomass of carp is approximately 50 g m 3 . The results suggest the applicability of a new food-web manipulation (increased stocking with filter-feeding fish) for controlling cyanobacteria blooms in hypereutrophic lakes. The approach differs from traditional biomanipulation in Europe and North America, where piscivores are added to control planktivores, and this in turn increases zooplankton and decreases algae. The new biomanipulation method is being used or being tested to counteract cyanobacteria blooms in many Chinese lakes such as Lake Dianchi?Xie and Liu: Biomanipulation to Control Cyanobacteria TheScientificWorld (2001) 1, 337-356 in Yunnan Province, Lake Chaohu in Anhui Province, and Lake Taihu in Jiangsu Province. The method has great potential as an important component of an integrated approach to counteract cyanobacteria blooms, especially in lakes where nutrient inputs cannot be reduced sufficiently, and where zooplankton cannot effectively control phytoplankton production.
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Debone, Daniela, Mariana V. da Costa, and Simone G. E. K. Miraglia. "90 Days of COVID-19 Social Distancing and Its Impacts on Air Quality and Health in Sao Paulo, Brazil." Sustainability 12, no. 18 (2020): 7440. http://dx.doi.org/10.3390/su12187440.
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The COVID-19 pandemic has imposed a unique situation for humanity, reaching up to 5623 deaths in Sao Paulo city during the analyzed period of this study. Due to the measures for social distancing, an improvement of air quality was observed worldwide. In view of this scenario, we investigated the air quality improvement related to PM10, PM2.5, and NO2 concentrations during 90 days of quarantine compared to an equivalent period in 2019. We found a significant drop in air pollution of 45% of PM10, 46% of PM2.5, and 58% of NO2, and using a relative-risk function, we estimated that this significant air quality improvement avoided, respectively, 78, 337, and 387 premature deaths, respectively, and prevented approximately US $720 million on health costs. Moreover, we estimated that 5623 deaths by COVID-19 represent an economic health loss of US $10.5 billion. Both health and economic gains associated with air pollution reductions give a positive perspective of the efforts towards keeping air pollution reduced even after the pandemic, highlighting the importance of improving the strategies of air pollution mitigation actions, as well as the crucial role of adopting efficient measures to protect human health both during and after the COVID-19 global health crisis.
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Smettem, KRJ, C. Kirkby, and DJ Chittleborough. "Hydrologic response of undisturbed soil cores to simulated rainfall." Soil Research 32, no. 6 (1994): 1175. http://dx.doi.org/10.1071/sr9941175.
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Experiments to characterize runoff and drainage from large undisturbed soil cores obtained from a site under volunteer pasture are presented. The objectives were to evaluate if a simple kinematic wave model could be used to characterize drainage under conditions of by-passing flow and to investigate the variability of runoff generation under wet and dry antecedent conditions in this well structured soil. Cores were taken to depths of 0.4 and 0.7 m, with six cores obtained from each depth. Results show that variations in soil structure between cores have a greater influence on the hydrologic response than variations in antecedent water content. It may be inferred that, in this soil, good structural continuity substantially enhances soil drainage and, in consequence, decreases surface runoff. During wetting, kinematic celerities ranged from 337 to 3200 mm h-1 in the short cores and from 357 to 1400 mm h-1 in the long cores. The corresponding estimated ranges of mobile or 'macropore' fractions were from 0.015 to 0.154 m3 m-3 in the short cores and 0.033 to 0.132 m3m-3 in the long cores. Both parameters were approximately log-normally distributed. Measured drainage hydrographs were reasonably well described by the kinematic model but the physical significance of the model parameters was unclear.
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Grubbs, R. D., C. A. Wetherill, K. Kutschke, and M. E. Maguire. "Magnesium transport in murine S49 lymphoma cells: pharmacology and divalent cation selectivity." American Journal of Physiology-Cell Physiology 248, no. 1 (1985): C51—C57. http://dx.doi.org/10.1152/ajpcell.1985.248.1.c51.
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The murine S49 lymphoma cell transports Mg2+ by a system distinct from systems responsible for Ca2+ influx (J. Physiol. London 337: 351-371, 1983). We have now determined the ability of various cations, anions, and drugs to modulate Mg2+ influx. Neither sulfate, nitrate, phosphate, nor bicarbonate altered Mg2+ influx. Among cations only T1+, Ba2+, Zn2+, Mn2+, Sc3+, and La3+ potently inhibited Mg2+ influx without causing obvious cell toxicity. Seventeen other cations were ineffective at maximal nontoxic concentrations. T1+ inhibition (Ki = 300 micron) is noncompetitive and apparently derives from its ability to dissipate membrane potential. The noncompetitive nature of and the rather poor inhibition constants for Ca2+ (Ki approximately equal to 5 mM) and Mn2+ (Ki = 200 micron) indicate that neither cation is an effective physiological antagonist of Mg2+ influx. Only Ba2+ exhibited competitive inhibition of Mg2+ influx (Ki = 1 mM). Cisplatin and Ca2+ channel antagonists also did not inhibit Mg2+ influx. These data further differentiate Mg2+ transport systems from those for Ca2+. In addition, the selectivity series for group IIa cation inhibition of influx (Mg2+ greater than Ba2+ much greater than Ca2+ greater than or equal to Sr2+) has not been observed previously in biological systems and is indicative of a very high anionic field strength at the Mg2+ recognition site.
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Kikkawa, U., S. K. Mann, R. A. Firtel, and T. Hunter. "Molecular cloning of casein kinase II alpha subunit from Dictyostelium discoideum and its expression in the life cycle." Molecular and Cellular Biology 12, no. 12 (1992): 5711–23. http://dx.doi.org/10.1128/mcb.12.12.5711.
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A Dictyostelium discoideum cDNA encoding an alpha-type subunit of casein kinase II was isolated, and its cDNA was used to study developmental expression of casein kinase II during the Dictyostelium life cycle. The 1.3-kb cDNA insert contained an open reading frame of 337 amino acids (M(r) 39,900). The deduced amino acid sequence has high homology with those of casein kinase II alpha subunits from other species. Genomic Southern blot analysis suggested that there is a single gene encoding casein kinase II alpha subunit in D. discoideum. Northern (RNA) blot analysis showed that the casein kinase II alpha-subunit gene is expressed constitutively as a 1.9-kb mRNA throughout vegetative growth and multicellular development. Casein kinase purified from normal vegetative cells contained a major protein band of approximately 36 kDa, which was recognized by antisera raised against rat testis casein kinase II. Comparison of the in vitro transcription/translation product of the alpha-subunit cDNA clone and the purified 36-kDa protein by partial proteolysis indicated that the isolated cDNA clone encodes the Dictyostelium casein kinase II alpha subunit. No protein corresponding to a beta subunit was detected in purified casein kinase. Immunoblot analysis using anti-rat casein kinase II sera showed that the alpha subunit of casein kinase II is expressed constitutively like its mRNA during the life cycle of D. discoideum. Casein kinase II activity measured by using a specific peptide substrate paralleled the level of alpha subunit detected by immunoblotting during the life cycle, with a maximum variation of approximately 2-fold. We were unable to obtain disruptants of the casein kinase II alpha gene, suggesting that there is a single casein kinase II alpha gene, which is essential for vegetative growth of D. discoideum.
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Kikkawa, U., S. K. Mann, R. A. Firtel, and T. Hunter. "Molecular cloning of casein kinase II alpha subunit from Dictyostelium discoideum and its expression in the life cycle." Molecular and Cellular Biology 12, no. 12 (1992): 5711–23. http://dx.doi.org/10.1128/mcb.12.12.5711-5723.1992.
Full textAbstract:
A Dictyostelium discoideum cDNA encoding an alpha-type subunit of casein kinase II was isolated, and its cDNA was used to study developmental expression of casein kinase II during the Dictyostelium life cycle. The 1.3-kb cDNA insert contained an open reading frame of 337 amino acids (M(r) 39,900). The deduced amino acid sequence has high homology with those of casein kinase II alpha subunits from other species. Genomic Southern blot analysis suggested that there is a single gene encoding casein kinase II alpha subunit in D. discoideum. Northern (RNA) blot analysis showed that the casein kinase II alpha-subunit gene is expressed constitutively as a 1.9-kb mRNA throughout vegetative growth and multicellular development. Casein kinase purified from normal vegetative cells contained a major protein band of approximately 36 kDa, which was recognized by antisera raised against rat testis casein kinase II. Comparison of the in vitro transcription/translation product of the alpha-subunit cDNA clone and the purified 36-kDa protein by partial proteolysis indicated that the isolated cDNA clone encodes the Dictyostelium casein kinase II alpha subunit. No protein corresponding to a beta subunit was detected in purified casein kinase. Immunoblot analysis using anti-rat casein kinase II sera showed that the alpha subunit of casein kinase II is expressed constitutively like its mRNA during the life cycle of D. discoideum. Casein kinase II activity measured by using a specific peptide substrate paralleled the level of alpha subunit detected by immunoblotting during the life cycle, with a maximum variation of approximately 2-fold. We were unable to obtain disruptants of the casein kinase II alpha gene, suggesting that there is a single casein kinase II alpha gene, which is essential for vegetative growth of D. discoideum.
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Koval, Alina. "The reflection of the formation of the Hellenistic international order in the work of Plutarch „Comparative biographies”." Bulletin of Luhansk Taras Shevchenko National University, no. 6 (337) (2020): 124–37. http://dx.doi.org/10.12958/2227-2844-2020-6(337)-124-137.
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The article comprehensively deals with the evidence of the peculiarities of the formation of the Hellenistic international order in the IV century BC. The author notes that the development of the Ukrainian historiography, among other things, is characterized by a deep interest of historians in studying the problems of international interaction between Eastern and Western civilizations. The study of this question allows to find answers to key questions – whether it is possible to create a universal global order based on common political and humanitarian values, or is it just an utopia, because no unifying model can take into account all the specifics of unique cultures, initially doomed to failure. In this context, it is important for the author to consider the history of such interaction between civilizations, in particular during the creation of the Hellenistic regional order in the IV century BC. This aspect is covered in detail in the treatise „Comparative Biographies”, compiled by the ancient Greek writer Plutarch (approximately 46 - 120/127 AD), the analysis of which is the main purpose of the article. As a result of the study, the author concluded that the biography of Alexander the Great presented by Plutarch allows us to consider the process of creating a Hellenistic empire, which united the key territories of West and East, to identify the logic of certain actions of the commander. Plutarch's description also allowed the author to conclude that in creating a new international order in the East, Alexander faced the traditional challenges of the time – increasing conflict with the tribal aristocracy, which for a long time stood behind him and used for personal gain closeness to the king. As a way out of this crisis, Alexander was forced to resort to strengthening his power, in fact reproducing the foundations of Eastern despotism.
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Jaiman, Sunil, Roberto Romero, Percy Pacora, et al. "Disorders of placental villous maturation are present in one-third of cases with spontaneous preterm labor." Journal of Perinatal Medicine 49, no. 4 (2021): 412–30. http://dx.doi.org/10.1515/jpm-2020-0138.
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Abstract Objectives Spontaneous preterm labor is an obstetrical syndrome accounting for approximately 65–70% of preterm births, the latter being the most frequent cause of neonatal death and the second most frequent cause of death in children less than five years of age worldwide. The purpose of this study was to determine and compare to uncomplicated pregnancies (1) the frequency of placental disorders of villous maturation in spontaneous preterm labor; (2) the frequency of other placental morphologic characteristics associated with the preterm labor syndrome; and (3) the distribution of these lesions according to gestational age at delivery and their severity. Methods A case-control study of singleton pregnant women was conducted that included (1) uncomplicated pregnancies (controls, n=944) and (2) pregnancies with spontaneous preterm labor (cases, n=438). All placentas underwent histopathologic examination. Patients with chronic maternal diseases (e.g., chronic hypertension, diabetes mellitus, renal disease, thyroid disease, asthma, autoimmune disease, and coagulopathies), fetal malformations, chromosomal abnormalities, multifetal gestation, preeclampsia, eclampsia, preterm prelabor rupture of the fetal membranes, gestational hypertension, gestational diabetes mellitus, and HELLP (hemolysis, elevated liver enzymes and low platelet count) syndrome were excluded from the study. Results Compared to the controls, the most prevalent placental lesions among the cases were the disorders of villous maturation (31.8% [106/333] including delayed villous maturation 18.6% [62/333] vs. 1.4% [6/442], q<0.0001, prevalence ratio 13.7; and accelerated villous maturation 13.2% [44/333] vs. 0% [0/442], q<0.001). Other lesions in decreasing order of prevalence included hypercapillarized villi (15.6% [68/435] vs. 3.5% [33/938], q<0.001, prevalence ratio 4.4); nucleated red blood cells (1.1% [5/437] vs. 0% [0/938], q<0.01); chronic inflammatory lesions (47.9% [210/438] vs. 29.9% [282/944], q<0.0001, prevalence ratio 1.6); fetal inflammatory response (30.1% [132/438] vs. 23.2% [219/944], q<0.05, prevalence ratio 1.3); maternal inflammatory response (45.5% [195/438] vs. 36.1% [341/944], q<0.01, prevalence ratio 1.2); and maternal vascular malperfusion (44.5% [195/438] vs. 35.7% [337/944], q<0.01, prevalence ratio 1.2). Accelerated villous maturation did not show gestational age-dependent association with any other placental lesion while delayed villous maturation showed a gestational age-dependent association with acute placental inflammation (q-value=0.005). Conclusions Disorders of villous maturation are present in nearly one-third of the cases of spontaneous preterm labor.
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Burns, James M., Eric K. Adeeku, and Patricia D. Dunn. "Protective Immunization with a Novel Membrane Protein of Plasmodium yoelii-Infected Erythrocytes." Infection and Immunity 67, no. 2 (1999): 675–80. http://dx.doi.org/10.1128/iai.67.2.675-680.1999.
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ABSTRACT Immunization with a particulate fraction of blood-stage antigens was shown previously to protect mice against Plasmodium yoelii malaria. To identify antigens inducing the protective response, sera from immunized mice were used to screen a P. yoelii cDNA expression library. Sequence analysis of one 2.6-kb cDNA clone indicated that the identified gene,pypag-1, encoded a novel plasmodial antigen. Two nonoverlapping regions of pypag-1 were expressed inEscherichia coli. The first recombinant antigen, pAg-1N, contained the N-terminal 337 residues, which included a putative transmembrane domain and a region relatively rich in tryptophan residues. The second recombinant antigen, pAg-1C, contained the remaining C-terminal 211 residues, which included 31 copies of a 5-amino-acid degenerative repeat. Immunoblot studies using rabbit antiserum raised against recombinant pAg-1N showed that the native pypAg-1 protein migrated at approximately 98 kDa, considerably slower than its predicted molecular mass of 66 kDa. Immunofluorescence studies localized the expression of the native pypAg-1 protein both to the cytoplasm and at the surface of P. yoelii-infected erythrocytes. Immunization with either pAg-1N or pAg-1C induced a four- to sevenfold reduction in P. yoeliiblood-stage parasitemia. As such, pypAg-1 appears to contain at least two distinct protective epitopes. To our knowledge, this is the first characterization of a protective antigen of P. yoelii that is associated with the erythrocyte membrane.
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Albert, Dara V. F., Rohit R. Das, Jayant N. Acharya, et al. "The Impact of COVID-19 on Epilepsy Care: A Survey of the American Epilepsy Society Membership." Epilepsy Currents 20, no. 5 (2020): 316–24. http://dx.doi.org/10.1177/1535759720956994.
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The COVID-19 pandemic has impacted the delivery of care to people with epilepsy (PWE) in multiple ways including limitations on in-person contact and restrictions on neurophysiological procedures. To better study the effect of the pandemic on PWE, members of the American Epilepsy Society were surveyed between April 30 and June 14, 2020. There were 366 initial responses (9% response rate) and 337 respondents remained for analysis after screening out noncompleters and those not directly involved with clinical care; the majority were physicians from the United States. About a third (30%) of respondents stated that they had patients with COVID-19 and reported no significant change in seizure frequency. Conversely, one-third of respondents reported new onset seizures in patients with COVID-19 who had no prior history of seizures. The majority of respondents felt that there were at least some barriers for PWE in receiving appropriate clinical care, neurophysiologic procedures, and elective surgery. Medication shortages were noted by approximately 30% of respondents, with no clear pattern in types of medication involved. Telehealth was overwhelmingly found to have value. Among the limitation of the survey was that it was administered at a single point in time in a rapidly changing pandemic. The survey showed that almost all respondents were affected by the pandemic in a variety of ways.
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Humphrey, Dalton, Spenser Becker, Jason Lee, Keith Haydon, and Laura L. Greiner. "176 Evaluation of a novel threonine source for nursery pig diets." Journal of Animal Science 98, Supplement_3 (2020): 85–86. http://dx.doi.org/10.1093/jas/skaa054.151.
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Abstract Four hundred and eighty (PIC 337 X 1050, PIC Genus, Hendersonville, TN) pigs were used to evaluate a novel threonine source (ThrPro, CJ America-Bio, Fort Dodge, IA) for nursery pigs from approximately 7 kg to 20 kg. At weaning, pigs were sorted by gender and fed a common diet for one week. Upon completion of the first week, pigs were sorted into randomized complete blocks, equalized by weight, within 16 replications. Pigs were allocated to one of three dietary treatments: positive control (POS)-SID LYS:THR 0.60, negative control (NEG)-SID LYS:THR ≤0.46 and alternative threonine source (TEST)-SID LYS:THR 0.60. All other nutrients met or exceeded the NRC (2012) recommendations. Growth and intake data were analyzed using PROC MIXED procedure in SAS. The experimental unit was the pen. During the first 14 days, pigs fed TEST had similar ADG (0.417 vs. 0.414 kg/d, P=0.81) and G:F (0.977 vs. 1.030, P=0.18) compared to POS, and increased ADG (0.417 vs. 0.387 kg/d, P=0.01) and G:F (0.977 vs. 0.898, P=0.05) compared to NEG. Over days 14-28, pigs fed TEST had similar ADG (0.523 vs. 0.532 kg/d, P=0.49) and G:F (0.712 vs. 0.707, P=0.71) compared to POS, and increased ADG (0.523 vs. 0.479 kg/d, P=0.002) and G:F (0.712 vs. 0.627, P< 0.0001) compared to NEG. Overall (d 0 to 28), pigs fed TEST had similar ADG (0.466 vs. 0.474 kg/d, P=0.48) and G:F (0.808 vs. 0.816, P=0.55) compared to POS, and increased ADG (0.466 vs. 0.433 kg/d, P=0.002) and G:F (0.808 vs. 0.725, P< 0.0001) compared to NEG. ADFI was not significantly different across treatments for the entirety of the study. In conclusion, the replacement of L-threonine with a novel threonine source resulted in similar growth performance in nursery pigs from approximately 7 kg to 20 kg.
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Zhang, Hanchao, Xiaogang Ning, Zhenfeng Shao, and Hao Wang. "Spatiotemporal Pattern Analysis of China’s Cities Based on High-Resolution Imagery from 2000 to 2015." ISPRS International Journal of Geo-Information 8, no. 5 (2019): 241. http://dx.doi.org/10.3390/ijgi8050241.
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The urbanization level in China has increased rapidly since beginning of the 21st century, and the monitoring and analysis of urban expansion has become a popular topic in geoscience applications. However, problems, such as inconsistent concepts and extraction standards, low precision, and poor comparability, existing in urban monitoring may lead to wrong conclusions. This study selects 337 cities at the prefecture level and above in China as research subjects and uses high-resolution images and geographic information data in a semi-automatic extraction method to identify urban areas in 2000, 2005, 2010, and 2015. City size distribution patterns, urban expansion regional characteristics, and expansion types are analyzed. Results show that Chinese cities maintained a high-speed growth trend from 2000 to 2015, with the total area increasing by 115.79%. The overall scale of a city continues to expand, and the system becomes increasingly complex. The urban system is more balanced than the ideal Zipf distribution, but it also exhibited different characteristics in 2005. Urban areas are mostly concentrated in the eastern and central regions, and the difference between the east and the west is considerable. However, cities in the western region continuously expand. Beijing, Shanghai, Tianjin, and Guangzhou are the four largest cities in China. Approximately 73.30% of the cities are expanding in an extended manner; the urban form tends to be scattered, and land use efficiency is low. The new urban areas mainly come from cultivated land and ecological land.
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Nicholas, K. R., та C. H. Tyndale-Biscoe. "Prolactin-dependent accumulation of α-lactalbumin in mammary gland explants from the pregnant tammar wallaby (Macropus eugenii)". Journal of Endocrinology 106, № 3 (1985): 337–42. http://dx.doi.org/10.1677/joe.0.1060337.
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ABSTRACT The minimal hormonal requirements for the in-vitro accumulation of α-lactalbumin have been investigated in a marsupial, the tammar (Macropus eugenii). Mammary gland explants from 24-day pregnant tammars cultured in medium containing bovine insulin, cortisol and ovine prolactin showed a progressive increase in accumulation of α-lactalbumin during 4 days of incubation. No increment was observed if prolactin was omitted from the medium. However, a similar rate of increase was observed after 3 days of culture in medium containing prolactin alone. This induction of α-lactalbumin was maximal at a prolactin concentration of approximately 0·02 mg/l, which corresponds to physiological levels during pregnancy and early lactation. The absence of an effect of bovine insulin on tammar explants is not due to a general unresponsiveness to this hormone since insulin-stimulated DNA synthesis and amino acid uptake was evident after 3 days of culture. The inclusion of tri-iodothyronine and raised concentrations of cortisol in culture media have been shown to modulate α-lactalbumin synthesis in eutherian mammals but were without effect in the tammar. In addition, increased levels of progesterone did not inhibit the induction of α-lactalbumin, confirming an earlier invivo study suggesting that progesterone withdrawal may not be the lactogenic trigger in this species. Thus the pregnant tammar is the only species yet described in which α-lactalbumin is induced maximally in vitro in response to a single hormone. J. Endocr. (1985) 106, 337–342
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Thompson, Robyn, Brandon Knopf, Laura Greiner, and Jon R. Bergstrom. "147 Effects of added dietary Zn and Cu or an essential oils blend in a nursery feeding program." Journal of Animal Science 97, Supplement_2 (2019): 83. http://dx.doi.org/10.1093/jas/skz122.151.
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Abstract Approximately 1,200 weaned pigs (8.8 ± 1 kg; Camborough×337, PIC, Hendersonville, TN) were used to evaluate the effects of a blend of essential oils compared to therapeutic programs [negative control (NC), Carbodox (NC + 55 ppm Mecadox, Phibro, Teaneck, NJ; MX), added ZnO and CuSO4 (NC + 2,000 ppm Zn and 150 ppm Cu in P1 and 150 ppm Cu in P2; ZnCu), and essential oils blend (NC + 0.4% Victus Liv, DSM Nutritional Products, Parsippany, NJ; VL)]. Pigs were allotted to the treatments 10 d after weaning. Treatments occurred over 2 phases: 14 d (P1) and 21 d (P2). Average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency (F:G) were evaluated. Data were analyzed as a CRD using the PROC MIXED procedure of SAS with pen as the experimental unit and treatment as main effect. During P1, pigs fed MX or ZnCu had increased ADG compared with NC (P = 0.001 and P = 0.003, respectively) and VL (P = 0.007 and P = 0.018, respectively). Pigs fed MX, VL, or ZnCu had improved F:G compared to NC (P < 0.0001, P = 0.04, and P = 0.001, respectively). In P2, ZnCu or MX increased ADG compared to VL (P = 0.03, P = 0.06, respectively) and ZnCu tended to increase ADG compared to NC (P = 0.07). Overall (d 0 to 35), VL tended to decrease the percent removals compared with NC (P = 0.07). In conclusion, feeding MX or ZnCu improved ADG and F:G and diets with VL resulted in the least removals.
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Maric, Dora, Antonino Bianco, Ivan Kvesic, Damir Sekulic, and Natasa Zenic. "Analysis of the Relationship between Tobacco Smoking and Physical Activity in Adolescence: A Gender Specific Study." Medicina 57, no. 3 (2021): 214. http://dx.doi.org/10.3390/medicina57030214.
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Background and Objective: Although smoking and the physical activity level (PAL) are important determinants of health status in adolescence, there is a lack of information on the relationship between smoking and PAL in early adolescence. The objective of this study was to evaluate the gender-specific relationship between smoking and PAL in 14-to-16-year-old adolescents. Materials and Methods: The sample included 650 adolescents (337 girls, 14.7 ± 0.5 years at first testing wave) from Bosnia and Herzegovina. During the first testing wave, participants were tested using structured questionnaires. Second testing was commenced after approximately 20 months (16.4 ± 0.6 years). The variables were age, gender, socioeconomic status, living environment, cigarette smoking (predictors), and PAL (criterion). Predictors were measured at the first wave, and criterion at the first-wave and second-wave. Results: For girls, smoking was negatively correlated to PAL at the first-wave (OR: 0.75, 95% CI: 0.55–0.95) and at the second-wave (OR: 0.73, 95% CI: 0.71–0.96). No significant association between smoking and PAL was found for boys. Results suggest that adolescent boys and girls do not follow the equal trajectories when it comes to relationships between smoking and PAL. Conclusions: In developing promotional public health actions related to a decrease of smoking and increase of PAL, a gender-specific approach is highly recommended. Further studies analyzing the cause–effect relationship between consumption of other types of psychoactive substances and PAL in this age group are warranted.
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Corlett, Richard T. "Characteristics of vertebrate-dispersed fruits in Hong Kong." Journal of Tropical Ecology 12, no. 6 (1996): 819–33. http://dx.doi.org/10.1017/s0266467400010075.
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ABSTRACTHong Kong has a native angiosperm flora of approximately 1800 species, of which 27% (482 spp.) bear fleshy, presumably vertebrate-dispersed fruits, including 76% of the 337 tree and shrub species and 70% of the 103 climber species. Morphological characteristics were determined for 255 species and nutritional characteristics of the fruit pulp for 153 species. Most fruit species were black (45.1%) or red (24.3%) and 85.9% had a mean diameter <13 mm. Nutritional characteristics varied widely between species with ranges and median values as follows: pulp percentage (range 10.0–99.2%, median 69.2%), water content of pulp (11.1–94.0%, 78%), lipid (0–84.0%, 2.0%), soluble carbohydrate (4–88%, 53%), nitrogen (0.2–3.4%, 0.86%), neutral detergent fibre (1–44%, 14.3%). Fruit development time (50–360 d, 156 d) showed a negative correlation with lipid content, but no significant correlation with fruit or seed size. Principal components analysis of fruit characteristics was dominated by a trend from single-seeded fruits with a thin, lipid-rich pulp layer to multiple-seeded fruits with much, watery, carbohydrate-rich pulp. Birddispersed species cover the full range of fruit characteristics except those too large to swallow and too hard to peck bits from. Mammals (bats, civets and/or macaques) are known or suspected to consume most of the fruits too large for birds as well as many bird fruits but none with high-lipid content. Summer fruits (May—September) were significantly larger and had significantly higher seed size and carbohydrate content than winter fruits (November-March). Winter fruits took more than twice as long to develop as summer fruits.
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Mo, Xing-Bo, Huan Zhang, Ai-Li Wang, Tan Xu, and Yong-Hong Zhang. "Integrative analysis identifies the association between CASZ1 methylation and ischemic stroke." Neurology Genetics 6, no. 5 (2020): e509. http://dx.doi.org/10.1212/nxg.0000000000000509.
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ObjectiveTo highlight potential epigenetic risk factors for blood pressure (BP) and ischemic stroke (IS) in loci identified by genome-wide association studies (GWASs).MethodsWe detected DNA methylation for BP (317,756 individuals from UK Biobank) and IS (521,612 individuals from MEGASTROKE) in Europeans by using the summary data–based mendelian randomization (SMR) method. We selected the most relevant gene to validate the association in 1,207 patients with hypertensive IS and 1,269 controls from the Chinese populations.ResultsWe first identified 173 CpG sites in 90 genes, 337 CpG sites in 142 genes, and 9 CpG sites in 7 genes that were significantly associated with systolic, diastolic BP, and IS, respectively. The methylation level of cg12760995 in CASZ1 was associated with systolic (PSMR = 1.74 × 10−12), diastolic BP (PSMR = 2.48 × 10−10), and IS (odds ratio [OR] = 0.92 [95% confidence interval [CI]: 0.91–0.94]; PSMR = 2.28 × 10−8) in Europeans. The methylation levels of 17 sites in the promoter of CASZ1 were measured in the Chinese individuals, and 10 of them were significantly associated with IS. The higher methylation level of CASZ1 was associated with a lower risk of IS (adjusted OR = 0.97 [95% CI: 0.96–0.99]). CASZ1 seemed to be hypomethylated in hypertensive cases, and the level was negatively correlated with BP. Systolic and diastolic BP mediated approximately 61.2% (p = 3.49 × 10−6) and 45.0% (p = 0.0029) of the association between CASZ1 methylation and IS, respectively.ConclusionsThis study identified DNA methylations that were associated with BP and IS. CASZ1 was hypomethylated in Chinese patients with hypertensive IS.
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Bůžková, Petra, Joshua I. Barzilay, Howard A. Fink, John A. Robbins, Jane A. Cauley, and Annette L. Fitzpatrick. "Ratio of Urine Albumin to Creatinine Attenuates the Association of Dementia With Hip Fracture Risk." Journal of Clinical Endocrinology & Metabolism 99, no. 11 (2014): 4116–23. http://dx.doi.org/10.1210/jc.2014-2409.
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Context: Microvascular disease is a leading cause of cognitive impairment. Approximately 50% of people with a hip fracture have cognitive impairment. Objective: We tested the hypothesis that microvascular diseases of the brain (lacunar infarcts and white matter disease [WMD]), kidney (albuminuria [≥ 30 mg/g creatinine] and albumin creatinine ratio [ACR]), and eye (retinal vascular disorders) attenuate the association of cognitive impairment with hip fracture risk. Setting: The Cardiovascular Health Cognition Study. Patients: Three thousand, one-hundred six participants (mean age, ∼79 y; 8.84 y median follow-up) with cognitive testing. Subsets received ACR testing (n=2389), brain magnetic resonance imaging scans (n = 2094), and retinal photography (n = 1098). Main Outcome Measure: Incident hip fracture. Results: There were 488 participants (16%) with mild cognitive impairment (MCI) and 564 (18%) with dementia. There were 337 incident hip fractures, of which 19% occurred in participants with MCI and 26% in participants with dementia. Adjusted hazard ratios (HR) and 95% confidence interval for hip fracture in participants with MCI were 2.45 (1.67–3.61) and for dementia 2.35 (1.57–3.52). With doubling of ACR, the HR for fracture was attenuated in participants with dementia compared with participants with normal cognition [interaction HR 0.70 (0.55–0.91)]. No such effect was found in participants with MCI. Albuminuria, lacunar infarcts, WMD, and retinal vascular disease (RVD) did not modify the association of dementia or MCI with hip fracture risk. Conclusions: ACR attenuates part of the risk of hip fracture in people with dementia, suggesting that these disorders share a common pathogenesis.
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Saha, A. K., T. G. Kurowski, J. R. Colca, and N. B. Ruderman. "Lipid abnormalities in tissues of the KKAy mouse: effects of pioglitazone on malonyl-CoA and diacylglycerol." American Journal of Physiology-Endocrinology and Metabolism 267, no. 1 (1994): E95—E101. http://dx.doi.org/10.1152/ajpendo.1994.267.1.e95.
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Insulin resistance is present in liver and muscle of subjects with type 2 diabetes and obesity. Recent studies suggest that such insulin resistance could be related to abnormalities in lipid-mediated signal transduction; however, the nature of these abnormalities is unclear. To examine this question further, tissue levels of diacylglycerol (DAG), malonyl-CoA, and triglyceride (TG) were determined in liver and soleus muscle of obese insulin-resistant KKAy mice and lean C57 BL control mice. In addition, the effects of treatment with pioglitazone, an antidiabetic agent that acts by increasing insulin sensitivity in muscle, liver, and other tissues, were assessed. The KKAy mice were hyperglycemic (407 vs. 138 mg/dl), hypertriglyceridemic (337 vs. 109 mg/dl), hyperinsulinemic (631 vs. 15 mU/ml), and weighed more (42 vs. 35 g) than the control mice. They also had 1.5- to 2.0-fold higher levels of malonyl-CoA in both liver and muscle, higher DAG (twofold) and TG (1.3-fold) levels in muscle, and higher TG (threefold), but not DAG, levels. Treatment of the KKAy mice with pioglitazone for 4 days decreased plasma glucose, TGs, and insulin by approximately 50% and restored hepatic and muscle malonyl-CoA levels to control values. In contrast, pioglitazone increased hepatic and muscle DAG levels two- or threefold. It has no effect on muscle or hepatic TG content, and it slightly increased hepatic TGs in the control group. The results indicate that abnormalities in tissue lipids occur in both liver and muscle of the KKAy mouse and that they are differentially altered when insulin sensitivity is enhanced by treatment with pioglitazone.(ABSTRACT TRUNCATED AT 250 WORDS)
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Lee, I. M., K. D. Bottner, and M. C. Black. "First Report of Yucca Phyllody Associated with 16SrI-A Phytoplasmas in Texas." Plant Disease 91, no. 4 (2007): 467. http://dx.doi.org/10.1094/pdis-91-4-0467c.
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Buckley's yucca (Yucca constricta Buckl.) is a native flowering perennial plant widely distributed in Texas and northeast Mexico. It is also grown as an ornamental plant in its native range as well as in other dry regions in the United States and Mexico. In 2006, during an extended drought, Buckley's yucca plants sporadically exhibited phyllody and abnormal bud proliferation on the inflorescence in Uvalde County in southwestern Texas. Symptoms resembled those caused by phytoplasmal infection. Samples from four symptomatic and two asymptomatic yucca plants were collected. Total nucleic acid was extracted from abnormal bud tissue. To assess the etiological aspect of the disease nested PCR using phytoplasma specific primer pair P1/16S-SR or P1/P7 followed by R16F2n/R16R2n was employed for the detection of putative phytoplasmas (2). An amplicon of approximately 1.2 kb was amplified from all four symptomatic yucca plants but not from asymptomatic plants. Restriction fragment length polymorphism (RFLP) patterns of 16S rDNA digested singly with AluI, KpnI, HpaII, MseI, HhaI, and RsaI endonucleases indicated that affected yucca plants were infected by a phytoplasma belonging to aster yellows group 16SrI (‘Candidatus Phytoplasma asteris’), subgroup 16SrI-A (1). Nucleotide sequence analysis of cloned 16S rDNA (GenBank Accession No. EF190067) confirmed the results on the basis of RFLP analyses. Yucca phyllody has not been reported elsewhere. This disease appears to be newly emerging in Texas with only a few affected plants. To our knowledge, this is the first report of 16SrI-A phytoplasma infecting a Yucca sp. References: (1) I.-M. Lee et al. Int. J. Syst. Bacteriol. 48:1153, 1998. (2) I.-M. Lee et al. Int. J. Syst. Evol. Microbiol. 54:337, 2004.
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Filardo, Thomas D., Maria R. Khan, Noa Krawczyk, et al. "Comorbidity and clinical factors associated with COVID-19 critical illness and mortality at a large public hospital in New York City in the early phase of the pandemic (March-April 2020)." PLOS ONE 15, no. 11 (2020): e0242760. http://dx.doi.org/10.1371/journal.pone.0242760.
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Background Despite evidence of socio-demographic disparities in outcomes of COVID-19, little is known about characteristics and clinical outcomes of patients admitted to public hospitals during the COVID-19 outbreak. Objective To assess demographics, comorbid conditions, and clinical factors associated with critical illness and mortality among patients diagnosed with COVID-19 at a public hospital in New York City (NYC) during the first month of the COVID-19 outbreak. Design Retrospective chart review of patients diagnosed with COVID-19 admitted to NYC Health + Hospitals / Bellevue Hospital from March 9th to April 8th, 2020. Results A total of 337 patients were diagnosed with COVID-19 during the study period. Primary analyses were conducted among those requiring supplemental oxygen (n = 270); half of these patients (135) were admitted to the intensive care unit (ICU). A majority were male (67.4%) and the median age was 58 years. Approximately one-third (32.6%) of hypoxic patients managed outside the ICU required non-rebreather or non-invasive ventilation. Requirement of renal replacement therapy occurred in 42.3% of ICU patients without baseline end-stage renal disease. Overall, 30-day mortality among hypoxic patients was 28.9% (53.3% in the ICU, 4.4% outside the ICU). In adjusted analyses, risk factors associated with mortality included dementia (adjusted risk ratio (aRR) 2.11 95%CI 1.50–2.96), age 65 or older (aRR 1.97, 95%CI 1.31–2.95), obesity (aRR 1.37, 95%CI 1.07–1.74), and male sex (aRR 1.32, 95%CI 1.04–1.70). Conclusion COVID-19 demonstrated severe morbidity and mortality in critically ill patients. Modifications in care delivery outside the ICU allowed the hospital to effectively care for a surge of critically ill and severely hypoxic patients.
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Villalona-Calero, M., S. Goel, L. Schaaf, et al. "First-in-human phase I trial of a novel epothilone, KOS-1584." Journal of Clinical Oncology 24, no. 18_suppl (2006): 2003. http://dx.doi.org/10.1200/jco.2006.24.18_suppl.2003.
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2003 Background: KOS-1584 (9,10-didehydroepothilone D) was discovered as part of a screening program to develop a new generation of epothilones with higher potency and an improved pharmacologic/pharmacokinetic (PK) profile. Epothilones stabilize microtubule polymerization, inducing rapid G2/M arrest and apoptosis. Antitumor activity of KOS-1584 (Chou et al 2003) is approximately 3–12 fold more potent when compared to the structurally related Epothilone D. KOS-1584 demonstrates enhanced tumor tissue penetration and reduced exposure to selected tissues (including CNS). We report the results of the initial dose-escalation trial in which KOS-1584 was administered to pts with advanced solid malignancies. Methods: Define the MTD, toxicity profile and PK of KOS-1584 when administered via 3-hour infusion every 3 weeks. PK was determined after the 1st and 2nd infusion. Pharmacodynamics were assessed by serial sampling of PBMCs for microtubule bundle formation. Results: 27 pts (17 F; median age 60; median ECOG PS 1; median prior regimens 3, range 0–7) enrolled in 8 dose levels (between 0.8 - 11.3 mg/m2). To date, no Cycle 1 DLT has been seen. Toxicities (n=24) did not show obvious dose dependency; common toxicity (Grade 1–2) included gastrointestinal (diarrhea, constipation, nausea), fatigue, and ↑AST. Drug-related Grade 3 toxicity: constipation, fatigue and ↑AST (1 each). Drug-related neurotoxicity was not notable. PK/parent (n=25): t½ 17.7 ± 4.6h, Vz 741±330 L and CL 30.2± 16.5 L/h. At 8.5 mg/m2 Cmax 78 ± 29 ng/mL; AUCtot 631 ± 337 ng*h/mL. Cmax and AUCtot increased linearly with dose over the range tested. Vz is ∼5-fold and t½ 2-fold higher than that of Epothilone D. Dose dependent increases in microtubule bundle formation were observed (8.5 mg/m2: 40–50% at end of infusion, compared to 60–65% for ixabepilone and 50–60% for Epothilone D using the same assay at their phase 2 dose). A sigmoidal Emax model described the relationship between plasma concentration and microtubule bundle formation. Activity consisted of 4 pts with extended stable disease (6 cycles leiomyosarcoma and ovarian cancer; 5 cycles colon cancer; a 2nd patient with ovarian cancer is active at 5 cycles). Of these, 3 had document progressive disease prior to study. Conclusions: Accrual is continuing in order to define the optimal dose on the 3-week regimen. [Table: see text]
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Eady, R. R., T. H. Richardson, R. W. Miller, M. Hawkins, and D. J. Lowe. "The vanadium nitrogenase of Azotobacter chroococcum. Purification and properties of the Fe protein." Biochemical Journal 256, no. 1 (1988): 189–96. http://dx.doi.org/10.1042/bj2560189.
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1. Nitrogenase activity of a strain of Azotobacter chroococcum lacking the structural genes of Monitrogenase (nifHDK) was associated with a V + Fe-containing protein and an Fe-containing protein [Robson, Eady, Richardson, Miller, Hawkins & Postgate (1986) Nature (London) 322, 388-390; Eady, Robson, Richardson, Miller & Hawkins (1987) Biochem. J. 244, 197-207]. 2. The Fe protein was purified to homogeneity by the criterion of Coomassie Blue staining after electrophoresis in 10% or 17% (w/v) polyacrylamide gels in the presence of SDS. One type of subunit, of Mr 32,000 +/- 2000, was found. 3. The native protein had an Mr of 62,500 +/- 2500 and contained approximately 4 Fe atoms and 4 acid-labile sulphide groups per molecule. The amino acid composition was similar to those of other purified Fe proteins, and, characteristically, tryptophan was absent. The specific activities (nmol of protein/min per mg of protein) when assayed under optimum conditions with the VFe protein from this strain were 1211 for H2 evolution under Ar, 337 for NH3 from N2 formation and 349 for C2H2 reduction. Activity of the Fe protein was O2-labile with a t1/2 of 36 s in air. At low temperatures the dithionite-reduced protein exhibited e.p.r. signals consistent with the presence of both S = 1/2 and S = 3/2 spin states. These signals were similar to those given by other nitrogenase Fe proteins, as were the changes in their line shape that occurred in the presence of MgATP or MgADP. The absorbance spectra showed that an increase in absorption occurred in the visible range on reversible oxidation of the dithionite-reduced protein. The oxidized-minus-reduced epsilon 420 was 6000 M-1.cm-1.
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Kang, J., and J. Caprio. "Electrophysiological responses of single olfactory bulb neurons to amino acids in the channel catfish, Ictalurus punctatus." Journal of Neurophysiology 74, no. 4 (1995): 1421–34. http://dx.doi.org/10.1152/jn.1995.74.4.1421.
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1. Responses of 89 single olfactory bulb neurons from 43 channel catfish, Ictalurus punctatus, to amino acid odorants were recorded in vivo simultaneously with the electro-olfactogram (EOG). Recording time for individual neurons ranged from 16 to 344 min. The averaged spontaneous frequency ranged from x003C; 1 to 16 action potentials/s with a mean frequency of 5.2 +/- 3.6 (SD) action potentials/s. 2. Histological examinations of carbocyanine dye 1,1'diocadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI)-labeled olfactory bulbs and electrical stimulation of the olfactory tracts in a subset of experiments suggested that the majority of the recorded olfactory bulb neurons in this study were mitral cells. 3. Olfactory bulb neurons responded to amino acids with either an excitation or suppression of the background neural activity. Of the 337 stimulus applications, 28% of the responses were excitatory, and 33% were suppressive. The approximately 1:1 ratio of excitatory to suppressive responses for all stimulus applications suggests that suppressive responses also play important roles in the coding of odorant information in the channel catfish. 4. Responses of single olfactory bulb neurons were highly reproducible over time (up to 5 h). Responses to any amino acid never changed from excitation to suppression, or vice versa over time. 5. Single olfactory bulb neurons responded with excitation or suppression to more than one amino acid previously indicated to bind to independent receptors. 6. Estimated threshold concentrations for activation of an olfactory bulb neuron ranged from 10(-7) to 10(-3) M and were different from neuron to neuron for a particular stimulus and from stimulus to stimulus for a particular neuron. Responses of single olfactory bulb neurons to a given amino acid did not change from excitation to suppression, or vice versa, across different suprathreshold concentrations.
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Redman, Rebecca Ann, Douglas Coldwell, and Vivek R. Sharma. "Survival after Yttrium-90 radioembolization in elderly and nonelderly patients with hepatocellular carcinoma or colorectal cancer liver metastases." Journal of Clinical Oncology 33, no. 3_suppl (2015): 449. http://dx.doi.org/10.1200/jco.2015.33.3_suppl.449.
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449 Background: Systemic treatment of unresectable hepatocellular carcinoma (HCC) or colorectal cancer liver metastases (CLM) in the elderly can be complicated by increased toxicity. In addition, the increasing incidence of comorbidities with age may preclude surgical resection with curative intent. Hepatic arterial therapy is increasingly utilized in patients with HCC or CLM not amenable to surgical resection. Studies of transarterial chemoembolization in the elderly have generally shown similar safety and efficacy as compared to younger patients, although some studies suggest worse outcomes. The selective nature of radioembolization has the potential for improved tolerability in this patient population. Methods: We report the results of a retrospective review of patients with unresectable HCC or metastatic disease to the liver treated with Yttrium-90 radioembolization at a single institution. Results: Patients were referred for treatment after multidisciplinary evaluation, but were not treated as part of a clinical trial. A total of 94 patients treated were evaluable for follow up. There were approximately twice as many males as females (64% vs 36%). Elderly was defined as 70 years of age or older, representing 20 of the 94 patients. Average age of the elderly cohort was 76 (range 70-90), compared to 56 years of age (range 23-69) for the younger patients. Survival was measured from date of first radioembolization. Median survival was similar for elderly and younger patients when considering all tumor types (337 days vs 288 days). There was no difference in median survival between elderly and non-elderly patients with CLM (377 days vs 365 days) or with HCC (370 days vs 363 days). Conclusions: In our experience, survival after Yttrium-90 radioembolization in elderly and younger patients with primary HCC or CLM is similar. Age alone should not preclude consideration for liver-directed therapy.