Relevant bibliographies by topics / Aqueous two phase separation (ATPS)

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  2. Dissertations / Theses
  3. Books
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Academic literature on the topic 'Aqueous two phase separation (ATPS)'

Author: Grafiati
Published: 9 September 2021
Last updated: 1 February 2022

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Journal articles on the topic "Aqueous two phase separation (ATPS)"

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Kruse, Thomas, Axel Schmidt, Markus Kampmann, and Jochen Strube. "Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems." Antibodies 8, no. 3 (2019): 40. http://dx.doi.org/10.3390/antib8030040.

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Therapeutic monoclonal antibodies (mAb) are used for the treatment of numerous serious diseases, which have led to an increasing demand over the last decades. Increased cell density and mAb titer of the cultivation broth lead to great challenges for the subsequent clarification and capture operations in the downstream process. As an alternative approach to the conventional downstream process, a selective mAb extraction via an aqueous two-phase system (ATPS) directly from the cultivation broth of a mAb producing industrial relevant chinese hamster ovary (CHO) cell line was investigated. An efficient purification of the mAb was accomplished by the ATPS composition. The phase separation was realized by a newly developed membrane based phase separator. Moreover, a complete cell removal was integrated into this process by the used membrane. A selectivity between both phases was achieved by membrane modification. Yields up to 93% in the light phase and removal of process related impurities were obtained after aqueous two-phase extraction (ATPE). Phase separation performance as well as contact angles on the membrane were characterized for different ATPS. ATPE directly from the cultivation broth in combination with the new membrane based phase separation led to a mAb yield of 78% with a simultaneous reduction of deoxyribonucleic acid (DNA) and host cell protein (HCP) load.
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Ma, Chao, Xiao Dong Chen, Yin Qiu Kong, and Li Ming Che. "Phase Diagram of Aqueous Two-Phase System (ATPS) Composed of Polyethylene Glycol (PEG) and Gelatin." Advanced Materials Research 554-556 (July 2012): 286–94. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.286.

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Phase diagram of aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and gelatin is of paramount importance for the application of such system in microencapsulation of bioactive compounds. Phase separation of PEG/gelatin aqueous solution was investigated in the present work and the phase diagram of resultant ATPS was reported for the first time. The results show that phase separation will happen if the solid content of PEG/gelatin aqueous solution is higher than a critical value, resulting in an ATPS. The resultant ATPS consists of a low-density phase enriched in PEG and a dense phase enriched in gelatin. The phase compositions of the resultant ATPS were determined accurately using the method developed. The phase diagrams obtained show that higher solid content is required for the phase separation of PEG10,000/gelatin aqueous solution when compared with that of PEG20,000/gelatin one. And PEG is found to be more hydrophilic when compared with gelatin.
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Rogers, Bradley A., Kelvin B. Rembert, Matthew F. Poyton, et al. "A stepwise mechanism for aqueous two-phase system formation in concentrated antibody solutions." Proceedings of the National Academy of Sciences 116, no. 32 (2019): 15784–91. http://dx.doi.org/10.1073/pnas.1900886116.

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Aqueous two-phase system (ATPS) formation is the macroscopic completion of liquid–liquid phase separation (LLPS), a process by which aqueous solutions demix into 2 distinct phases. We report the temperature-dependent kinetics of ATPS formation for solutions containing a monoclonal antibody and polyethylene glycol. Measurements are made by capturing dark-field images of protein-rich droplet suspensions as a function of time along a linear temperature gradient. The rate constants for ATPS formation fall into 3 kinetically distinct categories that are directly visualized along the temperature gradient. In the metastable region, just below the phase separation temperature, Tph, ATPS formation is slow and has a large negative apparent activation energy. By contrast, ATPS formation proceeds more rapidly in the spinodal region, below the metastable temperature, Tmeta, and a small positive apparent activation energy is observed. These region-specific apparent activation energies suggest that ATPS formation involves 2 steps with opposite temperature dependencies. Droplet growth is the first step, which accelerates with decreasing temperature as the solution becomes increasingly supersaturated. The second step, however, involves droplet coalescence and is proportional to temperature. It becomes the rate-limiting step in the spinodal region. At even colder temperatures, below a gelation temperature, Tgel, the proteins assemble into a kinetically trapped gel state that arrests ATPS formation. The kinetics of ATPS formation near Tgel is associated with a remarkably fragile solid-like gel structure, which can form below either the metastable or the spinodal region of the phase diagram.
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Atefi, Ehsan, Ramila Joshi, and Hossein Tavana. "Effect of Molecular Weight of Phase Polymers on Partition of Cells in Aqueous Two-Phase Systems." MRS Advances 2, no. 45 (2017): 2415–26. http://dx.doi.org/10.1557/adv.2017.425.

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ABSTRACTDue to their aqueous environment and biocompatible polymers, aqueous two-phase systems (ATPS) provide a mild environment for partition of cells. A comprehensive understanding of cell partition in ATPS will facilitate cell separation and fractionation in ATPS for downstream cell-based analytical applications and various cellular and molecular biology studies. We report the effect of molecular weight of phase polymers on partition of cells between two aqueous phases and their interface. We generate three different ATPS by dissolving polyethylene glycol and dextran of different molecular weights in a standard cell culture medium of fixed composition. After suspending cells in each ATPS, we quantify the number of cells partitioned into each segregated phase and the interface between the two phases. Importantly, we use two-phase solutions of an identical interfacial tension from each ATPS to avoid the effect of interfacial tension on cell partition. Our results indicate that decreasing the molecular weight of one of the phase polymers results in distribution of a greater number of cells toward the phase rich in that polymer. Regardless of molecular weight of polymers used, two-phase solutions made with higher concentrations of polymers cause a significant shift toward cell partition to the interface. This study elucidates the role of polymer molecular weight on cell partition in ATPS and offers formulations for rapid and effective cell partition.
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Pereira, David Y., Chloe M. Wu, So Youn Lee, Eumene Lee, Benjamin M. Wu, and Daniel T. Kamei. "Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media." SLAS TECHNOLOGY: Translating Life Sciences Innovation 24, no. 5 (2019): 515–26. http://dx.doi.org/10.1177/2472630319861369.

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In previous work, our group discovered a phenomenon in which a mixed polymer–salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper. While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components. However, since the mechanism of phase separation in porous media is not completely understood, introducing other ATPSs to paper is an unpredictable process that relies on trial and error experiments. This is especially true for polymer–polymer ATPSs in which the characteristics of the two phases appear quite similar. Therefore, our group aimed to develop semiquantitative guidelines for choosing ATPSs that can phase separate in paper. In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer–salt and micellar ATPSs in fiberglass paper. We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper. We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)–dextran ATPSs in paper and control the composition of the leading and lagging phases. We also, for the first time, successfully demonstrated the phase separation phenomenon in hydrogels, thereby extending its application and potential benefits to an alternative porous medium.
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Souza, Ranyere L., Sónia P. M. Ventura, Cleide M. F. Soares, João A. P. Coutinho, and Álvaro S. Lima. "Lipase purification using ionic liquids as adjuvants in aqueous two-phase systems." Green Chemistry 17, no. 5 (2015): 3026–34. http://dx.doi.org/10.1039/c5gc00262a.

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Liu, Shao Peng, Zhi Min Zong, Shan Shan Zhang, et al. "Study of Aqueous Two Phase System on Water Soluble Bio Oil." Advanced Materials Research 236-238 (May 2011): 49–53. http://dx.doi.org/10.4028/www.scientific.net/amr.236-238.49.

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This paper was aimed to study the aqueous two phase extraction (ATPE) on water soluble bio oil(SBO), in which ethanol, K2HPO4, NaH2PO4and (NH4)2SO4were selected to form the water soluble bio oil(SBO) aqueous two phase system (ATPS). After phase separation, the extraction rates and the volume ratios of the upper to lower layer of the ATPSs were investigeted. Both the upper and lower layers of these SBO ATPSs were analyzed by elemental analyzer and Fourier transformation infrared spectrometer (FTIR). The different extraction rates indicates the influnces of different salt during the phase saperation, of which NaH2PO4has less effect to SBO than the other two; K2HPO4may neutralize some organic acid; the lowest extraction rate of ethanol/(NH4)2SO4system indicates the effects of (NH4)2SO4to SBO. Too much ethanol will fech in more water, hence the optimizational ratio of the SBO ATPS should be SBO:salt:ethanol=10:10:5. In element analysis, the diclining of oxygen in all the upper layers and the increasing of nitrogion in both layers of (NH4)2SO4system indicates the abbility of the less oxygen components concentration of the ATPS and the reaction between (NH4)2SO4and SBO. According to FTIR test, the lack of absorption at 1515cm-1of all the lower layers indicates the completely transforming of phenols into the upper layers. Because of the high extraction rate and less influence to SBO, ethanol/NaH2PO4system may be the most suitable ATPS for SBO extraction.
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Dembczyński, Radosław, Wojciech Białas, and Tomasz Jankowski. "Determination of phase diagrams and thermoseparation behaviour of aqueous two-phase systems composed of ethylene oxide–propylene oxide random copolymer and potassium phosphate." Chemical and Process Engineering 33, no. 3 (2012): 411–29. http://dx.doi.org/10.2478/v10176-012-0036-8.

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Abstract The application of aqueous two-phase systems (ATPS) is a cost-effective and simple method of protein separation (including enzymes) from complex systems. The first stage of designing the protein purification process in an ATPS involves the identification of the conditions for the formation of a given extraction system. For this purpose, the conditions for the formation of ATPSs in a thermoseparating EO50PO50 polymer/potassium phosphates system have been studied. Factors determining the ATPS formation comprised: separation temperature (4ºC or 20ºC), phosphate solution pH (6, 7.5 or 9) as well as the concentration of NaCl introduced into the systems (0.085 M, 0.475 M and 0.85 M). ATPS without NaCl were prepared as well. The conditions for the formation of the primary EO50PO50/potassium phosphate ATPS were determined with their phase diagrams. It was observed that with an increase of phosphate pH and NaCl concentration in the system, there was a decrease of the EO50PO50 and phosphate concentrations necessary to form a primary ATPS. After the primary two-phase separation, the top phase (rich in the EO50PO50 polymer) was partitioned from the bottom phase (rich in phosphates). Next, by means of polymer phase thermoseparation, a secondary two-phase system was formed. In the secondary EO50PO50/phosphate ATPS, the bottom phase was formed by the concentrated EO50PO50 polymer (30-80% concentration), while the top phase by a solution composed mainly of water, containing phosphate ions and remains of EO50PO50 polymer (3-7%).
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Seo, Hanjin, Changwoo Nam, Eunseo Kim, Juhyun Son, and Hyomin Lee. "Aqueous Two-Phase System (ATPS)-Based Polymersomes for Particle Isolation and Separation." ACS Applied Materials & Interfaces 12, no. 49 (2020): 55467–75. http://dx.doi.org/10.1021/acsami.0c16968.

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Wu, Jiang, Ying Kai Tong, and Li Tong Ban. "Separation of Casein Phosphopeptides (a Functional Food Material) by Aqueous Two-Phase Systems." Advanced Materials Research 554-556 (July 2012): 1511–14. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.1511.

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PEG 10000 / (NH4)2SO4 ATPS was applied to extract casein phosphopeptides. By studying on the partitioning behavior of CPPs in this system, it is concluded that the purification fold of CPPs could reach 7.09 when the system consisted of 20% PEG 10000 and 10% (NH4)2SO4.
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Dissertations / Theses on the topic "Aqueous two phase separation (ATPS)"

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Balasubramaniam, Deepa. "Lysozyme Separation from Tobacco Extract by Aqueous Two-Phase Extraction." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/31272.

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Tobacco has long been considered as a host to produce large quantities of high-valued recombinant proteins. However, dealing with large quantities of biomass with a dilute concentration of product is a challenge for down-stream processing. Aqueous two-phase extraction (ATPE) has been used in purifying proteins from various sources. It is a protein-friendly process and can be scaled up easily. ATPE was studied for its applicability to recombinant protein purification from tobacco using egg white lysozyme as the model protein. Separate experiments with polyethyleneglycol(PEG)/salt/tobacco extract, and PEG/salt/lysozyme were carried out to determine the partition behavior of tobacco protein and lysozyme, respectively. Two level fractional factorial designs were used to study the effects of factors such as PEG molecular weight, PEG concentration, the concentration of phase forming salt, sodium chloride concentration, and pH on protein partitioning. The results showed that PEG/sodium sulfate system was most suitable for lysozyme purification. Detailed experiments were conducted by spiking lysozyme into the tobacco extract. The conditions with highest selectivity of lysozyme over native tobacco protein were determined using a response surface design. The purification factor was further improved by decreasing the phase ratio along the tie line corresponding to the phase compositions with the highest selectivity. Under selected conditions the lysozyme yield was predicted to be 87% with a purification factor of 4 and concentration factor of 14. The binodial curve and tie line corresponding to the optimal condition for lysozyme recovery for the PEG 3400/sodium sulfate system were developed. The selectivity at the optimal condition was experimentally determined to be 47 with a lysozyme yield of 79.6 % with a purification factor of 10 and a concentration factor of 20. From this study, ATPE was shown to be suitable for initial protein recovery and partial purification from transgenic tobacco.<br>Master of Science
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Periyannan, Rajeswari Prem Kumar. "Microfluidic aqueous two-phase system for continous partitioning of bacteria." Thesis, KTH, Skolan för bioteknologi (BIO), 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-149277.

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Prinz, Axel [Verfasser]. "Enzyme Separation Using Aqueous Two-Phase Extraction: Experiment, Model and Simulation / Axel Prinz." München : Verlag Dr. Hut, 2014. http://d-nb.info/1053859686/34.

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Hodgson, Christopher John. "Partitioning and separation of tPA (tissue plasminogen activator) in aqueous two-phase systems." Thesis, University of Reading, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334032.

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Ross, Kristin Coby. "Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction". Thesis, Virginia Tech, 2008. http://hdl.handle.net/10919/43471.

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Biopharmaceutical manufacturing is a rigorous and expensive process. Due to the medicinal nature of the product, a high purity level is required and several expensive purification steps must be utilized. Cost-effective production and purification is essential for any biopharmaceutical product to be successful and development of the fastest, most economical, and highest-yielding purification scheme is a constant engineering challenge. Commercial-scale purification schemes currently revolve around the use of multiple chromatography steps for the purification of biopharmaceutical products. Chromatography has many shortcomings including high cost, limited throughput, and complex scale up. The goal of this research was to develop an alternative, non-chromatography purification step for the separation of an acidic model protein, recombinant β-glucuronidase (rGUS), from transgenic tobacco with high yield and purity. Aqueous two-phase extraction (ATPE) is a powerful technique for separation and purification of proteins, and has the potential to replace an expensive chromatography step for the initial purification of recombinant proteins. ATPE enables high levels of target protein recovery and concentration while removing large amounts of impurities from the initial extract. Fractional factorial designs and response surface methodology were used to determine an optimized aqueous two-phase system for the purification of rGUS from transgenic tobacco. In a 13.4 % (w/w) PEG/18% (w/w) potassium phosphate system, 74% of the rGUS was recovered in the top PEG-rich phase while 90% of the native tobacco proteins were removed in the interphase and the bottom phase. A purification factor of about 20 was achieved in this process.<br>Master of Science
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Huaman?, Edgard Ronny Delgado. "Estudo da parti??o de Carbon Dots em sistemas aquosos bif?sicos: potencialidades para cat?lise de transfer?ncia de fase." UFVJM, 2017. http://acervo.ufvjm.edu.br/jspui/handle/1/1562.

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Data de aprova??o retirada da vers?o impressa do trabalho.<br>Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2018-01-04T19:50:32Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) edgard_ronny_delgado_huamani.pdf: 5898825 bytes, checksum: 78eee370de5fed297b3181e82d98f63d (MD5)<br>Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-01-17T18:40:25Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) edgard_ronny_delgado_huamani.pdf: 5898825 bytes, checksum: 78eee370de5fed297b3181e82d98f63d (MD5)<br>Made available in DSpace on 2018-01-17T18:40:25Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) edgard_ronny_delgado_huamani.pdf: 5898825 bytes, checksum: 78eee370de5fed297b3181e82d98f63d (MD5) Previous issue date: 2017<br>Neste trabalho, Carbon Dots (CD) oxidados foram preparados a partir de rea??es de desidrata??o/oxida??o ?cida da celulose, e posteriormente desoxigenados em meio alcalino e rea??o com sulfato de hidrazina. As caracteriza??es dos CD foram feitas com Microscopia eletr?nica de Transmiss?o, FTIR, titula??o potenciom?trica, Resson?ncia Magn?tica Nuclear de 13C e espectroscopia UV-Vis e fluoresc?ncia. Os resultados obtidos com estas caracteriza??es mostraram que a amostra oxidada apresenta um tamanho m?dio aproximadamente de 2,7 nm com uma significativa quantidade de grupos funcionais oxigenados ?cidos, dos quais 63% s?o ?cidos carbox?licos. Ap?s as rea??es de modifica??o superficial foi verificado que em meio alcalino ocorre somente uma elimina??o de nanoestruturas mais oxidadas, enquanto que na rea??o com hidrazina ocorre a redu??o de alguns grupos carbox?licos e ep?xidos com forma??o de grupos funcionais hidrazonas. Como consequ?ncia da modifica??o superficial, as propriedades ?pticas dos CD s?o alteradas significativamente. Com a redu??o, o band gap diminui e a energia da emiss?o aumenta, deslocando-se para a regi?o azul do espectro eletromagn?tico. Uma vez caracterizados, a parti??o de todos os CD preparados foi investigada em diferentes Sistemas Aquosos Bif?sicos (SAB), nos quais foram avaliados os efeitos dos c?tions e ?nions dos sais, dos pol?meros e do pH inicial do sistema no coeficiente de parti??o (K). Em adi??o foi avaliado o efeito da modifica??o superficial das nanopart?culas. A rela??o do K com comprimento da linha de amarra??o (CLA), o par?metro termodin?mico dos SAB foi estudado para todos os sistemas. Os resultados obtidos mostraram que a melhor separa??o ocorreu com o sistema PEO1500+sulfato de l?tio+?gua em pH=3 e usando os Carbon Dots reduzidos com hidrazina. O valor do K para este sistema foi de 64,4. Por outro lado, o menor valor de K foi 0,79, foi obtido para o sistema PEG1500+tartarato de s?dio+?gua em pH=6 e usando Carbon Dots oxidados. As an?lises detalhadas das fases superior e inferior de alguns destes sistemas com espectroscopia de fluoresc?ncia mostraram que os SAB s?o capazes, al?m de particionar, separar as nanopart?culas com diferentes propriedades ?pticas, as quais est?o diretamente associadas ?s propriedades superficiais e tamanho de part?culas. Por fim, n?s avaliamos a atividade catal?tica foto-Fentom dos CD na degrada??o do corante ani?nico ?ndigo de carmim e os resultados mostraram que os CD apresentam atividade fotocatal?tica neste sistema. Devido ? alta parti??o verificada para o sistema PEG1500+ sulfato de l?tio+?gua, este foi usado para avaliar a potencialidade de seu uso em cat?lise de transfer?ncia de fase usando CD como fotocatalisador e o corante ?ndigo de carmim como mol?cula modelo. Apesar da complexidade e da dificuldade de caracteriza??o dos produtos da foto-oxida??o no SAB, os resultados obtidos indicam que o sistema testado pode ser usado em rea??es de transfer?ncia de fase fotocatalisadas. Como conclus?o final, acredita-se que os resultados apresentados, em especial, a parti??o/sele??o das nanopart?culas com distintas propriedades ?pticas s?o de extrema import?ncia para o desenvolvimento de novas e eficazes aplica??es dos CD.<br>Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017.<br>In this work, oxidized Carbon Dots (CD) were prepared by a dehydration/oxidation reaction of cellulose and subsequently deoxygenated in alkaline medium and reaction with hydrazine sulfate. The CD characterizations were made with Transmission Electron Microscopy, FTIR, potentiometric titration. 13C Nuclear Magnetic Resonance, UV-Vis and fluorescence spectroscopy. The results obtained with these characterizations showed that the oxidized sample has an average size of 2.7 nm with a significant amount of acid oxygenated functional groups, of which 63% are carboxylic acids. After the superficial modification reactions, it was verified that in alkaline conditions only one elimination of more oxidized nanostructures occurs, whereas in the reaction with hydrazine the reduction of some carboxylic groups and epoxides with formation of hydrazones functional groups. As a consequence of the surface modification, the optical properties of the CD are significantly changed. The band gap decreases and the emission energy increases, shifting to the blue region of the electromagnetic spectrum. Once characterized, the partitioning of all CD prepared was investigated in differente Aqueous two-phase systems (ATPS), in which the effects of the cations and anions of salts, of the polymers and the initial pH of the system in the partition coefficient (K) were evaluated. In addition, the surface modification effect of the nanoparticles was evaluated. The relationship of K with Tie Line Length (TLL), the thermodynamic parameter of the ATPS was studied for all systems. The results showed that the best separation occurred with the system PEO1500 + lithium sulfate + water at pH = 3 and using the reduced carbon dots with hydrazine. The K value for this system was 64,4. On the other hand, the lowest K value was 0,79, it was obtained for the system PEG1500 + sodium tartrate + water at pH = 6 and using oxidized carbon dots. The detailed analysis of the upper and lower phases of some of these systems with fluorescence spectroscopy showed that the ATPS are capable, in addition to partition, to separate the nanoparticles with different optical properties, which are associated directly to the surface properties and particle size. Finally, we evaluated the photo-Fentom catalytic activity of the CD in the degradation of the anionic anion dye indigo carmine and the results showed that the CD present photocatalytic activity in this system. Due to the high partition found for the PEG1500 + lithium + water sulfate system, it was used to evaluate the potentiality of its use in phase transfer catalysis using CD as a photocatalyst and the indigo carmine dye as a model molecule. Despite the complexity and difficulty of characterizing the photo-oxidation products in ATPS the results indicate that the system tested can be used in photocatalysed phase transfer reactions. As a final conclusion, we believe that the results presented, in particular, the partitioning / selection of nanoparticles with different optical properties are of extreme importance for the new and effective applications of CD.
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Huang, Ri-Bo. "Selective separation of yeast proteins by differential product release, enzymitic lysis and aqueous two-phase systems." Thesis, University of Reading, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280269.

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Laurence, M. B. "Separation of insoluble biological material downstream from a two liquid (organic/aqueous) phase whole cell biotransformation reactor." Thesis, University College London (University of London), 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.508462.

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Besel, Elif. "Use Of Triton X-114 Aqueous Two Phase System For Recovery Of Mushroom (agaricus Bisporus) Polyphenoloxidase." Master's thesis, METU, 2003. http://etd.lib.metu.edu.tr/upload/1262971/index.pdf.

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Mushroom (Agaricus bisporus) polyphenoloxidase (PPO) (EC 1.14.18.1) was isolated and purified using an aqueous two phase system composed of octyl phenol (ethyleneglycol) 7-8 ether (Triton X-114/TX-114). TX-114 is a non-ionic surfactant which thermoseparates in water and forms an aqueous two phase system with a surfactant-depleted top phase and a surfactant-enriched bottom phase. Critical micelle concentration (CMC) and cloud point of the surfactant are 0.17 mM and 22&ordm<br>C respectively. The partitioning behavior of mushroom PPO in water/TX-114 aqueous two-phase systems was studied and the effects of TX-114 concentration, ionic strength, pH, temperature and crude extract preparation on PPO partitioning were studied. PPO generally partitioned to the surfactant-depleted top phase<br>whereas many other hydrophobic proteins and molecules partitioned to the surfactantenriched bottom phase. When two-step ultrafiltration was used as a pretreatment, complete enzyme recovery was achieved with all studied TX-114 concentrations. Moreover, about 5 fold purification was achieved by using 8% TX-114. The purification increased to 10 fold by using polyvinylpolypyrolidone (PVPP) at pH 7.0 with a recovery of 72%. However changing pH from 7.0 to 6.0 increased the purification factor and enzyme recovery to 15 fold and 100%, respectively. Addition of potassium or sodium salts caused PPO molecules to partition in the surfactantenriched bottom phase. Finally, crude enzyme can be concentrated besides being purified and recovered by doing aqueous two-phase separation at room temperature.
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Ferreira, Ana Maria da Conceição. "Separation of dyes with reversible aqueous biphasic systems." Master's thesis, Universidade de Aveiro, 2013. http://hdl.handle.net/10773/12572.

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Mestrado em Engenharia Química<br>The main objective of this work conveys on the study of reversible aqueous two-phase systems (ATPS), constituted by ionic liquids (ILs), and their potential application for the selective separation of dyes mainly used in the textile industry. The textile manufacturing is one of the main industries which discharges a heavy load of chemicals, especially large contents of dyes during the dying process through wastewaters, which results in severe environmental and economic concerns. In this context, this work focuses on the applicability of reversible ATPS, as well as on the development of more benign systems than those studied hitherto, as an alternative technique for the removal of dyes from wastewaters. Additionally, special attention was also given to the understanding of the molecular mechanisms which rule the partitioning of dyes between the coexisting phases of ATPS. IL-based ATPS display a widespread applicability in the extraction, concentration and purification of a large range of compounds, including dyes. Thus, this work started with investigations on the extraction of a set of dyes (chloranilic acid, indigo blue and sudan III) using more conventional ATPS composed of ILs and an organic/inorganic salt. At this stage, the influences of the IL chemical structure, the salting-out ability of the salt employed and the consequent pH of the aqueous medium were evaluated by the dyes extraction efficiencies. The results obtained reveal that a proper selection of the IL and salt can lead to the complete extraction of the three dyes studied for the IL-rich phase in a single-step procedure. After demonstrating the high capacity of ATPS formed by ILs to extract dyes from aqueous phases, it was studied the applicability of pH-triggered reversible ATPS in the selective separation of organic and inorganic dyes (sudan III and pigment blue 27). The reversibility of this type of ATPS was achieved by the manipulation of the speciation of the organic salt used. The results obtained confirm the reversibility behaviour of ATPS by a pH-driven phenomenon, at least for three times, as well as their selective separation capability with both dyes being extracted for opposite phases. Finally, and after demonstrating the existence of reversible pH-triggered ATPS, mixtures of a polymer and cholinium-based ILs combined with anions derived from carboxylic acids were investigated, foreseeing the search of more benign and biocompatible systems. The reversibility of these systems was achieved with the speciation of the IL anion as a function of the pH. These systems were finally evaluated in what concerns their performance for the extraction and selective separation of dyes (sudan III, pigment blue 27 and pigment 29), and the study revealed that IL-polymer systems are capable of selectively extract organic and inorganic dyes for opposite phases.<br>O principal objetivo do presente trabalho recai no estudo de sistemas aquosos bifásicos (SAB) reversíveis, constituídos por líquidos iónicos (LIs), bem como na sua potencial aplicação para a separação seletiva de corantes utilizados maioritariamente pela indústria têxtil. Esta é uma das principais indústrias que liberta uma grande quantidade de produtos químicos, destacando-se a descarga de elevadas quantidades de corantes através dos respetivos efluentes aquosos, o que é motivo de elevada preocupação tanto a nível ambiental como económico. Este trabalho centra-se no estudo da aplicabilidade de SAB reversíveis, através de variações de pH do meio aquoso, assim como na procura de SAB constituídos por LIs mais benignos que os estudados até ao momento, como uma técnica alternativa para a remoção de corantes de efluentes aquosos. Adicionalmente, também se deu um especial destaque à interpretação dos mecanismos que regem a partição de corantes entre as duas fases neste tipo de sistemas. Os SAB constituídos por LIs apresentam uma grande aplicabilidade na extração e purificação de uma vasta gama de compostos, incluindo corantes. Assim, iniciou-se o presente trabalho com a extração de um conjunto de corantes (ácido cloroanílico, azul indigo, sudão III) utilizando SAB mais convencionais e constituídos por LIs e um sal orgânico/inorgânico. Nesta etapa avaliou-se o efeito da estrutura química do LI sobre a capacidade de extração, assim como o efeito do sal e consequente pH do meio. Os resultados obtidos revelaram que uma seleção adequada do LI e do sal pode conduzir à extração completa dos três corantes estudados e num único passo operacional. Após demonstrar a elevada capacidade de SAB constituídos por LIs para extrair corantes de fases aquosas, estudou-se a aplicabilidade de SAB reversíveis, por variações de pH, na separação seletiva de corantes orgânicos e inorgânicos (sudão III e pigmento azul 27). A reversibilidade deste tipo de SAB foi conseguida com a manipulação da especiação do sal orgânico utilizado. Os resultados obtidos confirmaram a reversibilidade dos SAB, pelo menos até três vezes, por variação do pH, assim como uma capacidade de extração seletiva de cada um dos corantes para fases opostas. Por fim, e após confirmada a existência de SAB reversíveis, estudaram-se misturas de um polímero e LIs da família das colinas com aniões derivados de ácidos carboxílicos com o intuito de encontrar sistemas mais benignos e biocompatíveis. A variação do pH e reversibilidade neste tipo de sistemas foi conseguida com a especiação do anião do LI. Estes sistemas foram finalmente avaliados no que respeita à sua capacidade de extração e separação seletiva de corantes (sudão III, pigmento azul 27 e pigmento 29), e o estudo revelou que os sistemas LI-polímero conduzem a uma extração seletiva entre os corantes orgânicos e os pigmentos inorgânicos.
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Books on the topic "Aqueous two phase separation (ATPS)"

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Aqueous two-phase partitioning: Physical chemistry and bioanalytical applications. M. Dekker, 1995.

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Partition of cell particles and macromolecules: Separation and purification of biomolecules, cell organelles, membranes, and cells in aqueous polymer two-phase systems and their use in biochemical analysis and biotechnology. 3rd ed. Wiley, 1986.

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Rajni, Hatti-Kaul, ed. Aqueous two-phase systems: Methods and protocols. Humana Press, 2000.

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Micinski, Stanley. Electro-extraction of proteins in aqueous two-phase media. 1994.

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(Editor), John N. Abelson, Melvin I. Simon (Editor), Harry Walter (Editor), and Göte Johansson (Editor), eds. Aqueous Two-Phase Systems (Methods in Enzymology). Academic Press, 1994.

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Hatti-Kaul, Rajni. Aqueous Two-Phase Systems: Methods and Protocols (Methods in Biotechnology). Humana Press, 2000.

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Harry, Walter, Brooks Donald E, and Fisher Derek, eds. Partitioning in aqueous two-phase systems: Theory, methods, uses, and application to biotechnology. Academic Press, 1985.

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Frampton, John Paul, Brendan M. Leung, and Anderson Ho Cheung Shum, eds. The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering. Frontiers Media SA, 2020. http://dx.doi.org/10.3389/978-2-88963-356-2.

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Aveyard, Bob. Surfactants. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198828600.001.0001.

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Characteristically, surfactants in aqueous solution adsorb at interfaces and form aggregates (micelles of various shapes and sizes, microemulsion droplets, and lyotropic liquid crystalline phases). This book is about the behaviour of surfactants in solution, at interfaces, and in colloidal dispersions. Adsorption at liquid/fluid and solid/liquid interfaces, and ways of characterizing the adsorbed surfactant films, are explained. Surfactant aggregation in systems containing only an aqueous phase and in systems with comparable volumes of water and nonpolar oil are each considered. In the latter case, the surfactant distribution between oil and water and the behaviour of the resulting Winsor systems are central to surfactant science and to an understanding of the formation of emulsions and microemulsions. Surfactant layers on particle or droplet surfaces can confer stability on dispersions including emulsions, foams, and particulate dispersions. The stability is dependent on the surface forces between droplet or particle surfaces and the way in which they change with particle separation. Surface forces are also implicated in wetting processes and thin liquid film formation and stability. The rheology of adsorbed films on liquids and of bulk colloidal dispersions is covered in two chapters. Like surfactant molecules, small solid particles can adsorb at liquid/fluid interfaces and the final two chapters focus on particle adsorption, the behaviour of adsorbed particle films and the stabilization of Pickering emulsions.
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Book chapters on the topic "Aqueous two phase separation (ATPS)"

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Wikström, P., S. Flygare, and P. O. Larsson. "Magnetically Enhanced Aqueous Two-Phase Separation." In Separations Using Aqueous Phase Systems. Springer US, 1989. http://dx.doi.org/10.1007/978-1-4684-5667-7_72.

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Axelsson, Claes-Goran. "Separation of Chondrocytes in Aqueous Two-Phase Systems." In Separations Using Aqueous Phase Systems. Springer US, 1989. http://dx.doi.org/10.1007/978-1-4684-5667-7_22.

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Raymond, Frank D., and Derek Fisher. "Interfacial Events in Phase Separation and Cell Partitioning in Aqueous Two-Phase Systems." In Aqueous Biphasic Separations. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-1953-9_12.

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Kaul, Rajni, and Bo Mattiasson. "Integration of Aqueous Two-Phase Extraction with Other Separation Techniques." In Aqueous Biphasic Separations. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-1953-9_5.

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Karr, Laurel J., James M. Van Alstine, Robert S. Snyder, Steven G. Shafer, and J. Milton Harris. "Cell Separation by Immunoaffinity Partition in Aqueous Polymer Two-Phase Systems." In Separations Using Aqueous Phase Systems. Springer US, 1989. http://dx.doi.org/10.1007/978-1-4684-5667-7_34.

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Skuse, D. R., and D. E. Brooks. "Column Based Liquid/Liquid Separation of Cells Using Aqueous Polymeric Two-Phase Systems." In Separations Using Aqueous Phase Systems. Springer US, 1989. http://dx.doi.org/10.1007/978-1-4684-5667-7_63.

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Franco, T., B. A. Andrews, O. Cascone, C. Hodgson, A. T. Andrews, and J. A. Asenjo. "Affinity Separation of Proteins in Aqueous Two-Phase Systems." In Separations for Biotechnology 2. Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-0783-6_36.

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Fisher, Derek, F. D. Raymond, and H. Walter. "Factors in Cell Separation by Partitioning in Two-Polymer Aqueous-Phase Systems." In ACS Symposium Series. American Chemical Society, 1991. http://dx.doi.org/10.1021/bk-1991-0464.ch012.

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Wen-Yih, Chen, Shu Ching-Gji, and Chen Jw-Yann. "The Effect of Amino Acid Sequence on the Separation of Peptides in an Aqueous Two- Phase System." In Biochemical Engineering for 2001. Springer Japan, 1992. http://dx.doi.org/10.1007/978-4-431-68180-9_159.

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Albertsson, Per-Åke, Göte Johansson, and Folke Tjerneld. "Aqueous Two-Phase Separations." In Separation Processes in Biotechnology. CRC Press, 2020. http://dx.doi.org/10.1201/9781003066392-13.

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Conference papers on the topic "Aqueous two phase separation (ATPS)"

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Wijethunga, Pavithra A. L., and Hyejin Moon. "A Study of On-Chip Aqueous Two Phase System Formation and its Applications." In ASME 2012 Third International Conference on Micro/Nanoscale Heat and Mass Transfer. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/mnhmt2012-75138.

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Although there are several reports demonstrating the use of aqueous two-phase systems (ATPS) for biological sample separation in miniaturized devices, they only used ATPS prepared in macro scale by following the conventional way. Formation of ATPS in a confined micro scale environment and their characteristics has not been studied yet. While ATPS formation in macro scale is supported by vigorous mixing and gravitational force to form two phases, both mixing capability and gravitational force aid are insignificant in micro scale. Hence, in this study, we investigated the formation of ATPS under these inferior conditions of micro scale. An electrowetting on a dielectric (EWOD) digital microfluidic chip was used as the confined miniaturized environment. In an EWOD device, micro/nano scale droplets are manipulated on an array of electrodes by sequential application of voltages to the electrodes. The common polyethylene glycol (PEG) and dextran (DEX) polymer/polymer ATPS was used as the model system. Nanoliter volume droplets of the two pure polymer solutions were brought into contact, mixed, and successfully formed ATPS on an EWOD chip. Furthermore, it was possible to separate the two phases into two droplets, demonstrating the promising capability of on-chip biological sample separations effectively by integrating formation of ATPS and separation process using ATPS in a single chip. This study also compares the characteristics of ATPS generated in microfluidic chip with that generated in conventional macro scale. The effect of mixing performance on the ATPS formation is studied experimentally.
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Mastiani, Mohammad, Seokju Seo, Sofia Melgar Jimenez, Nick Petrozzi, and Myeongsub (Mike) Kim. "Understanding Fundamental Physics of Aqueous Droplet Generation Mechanisms in the Aqueous Environment." In ASME 2017 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/imece2017-71542.

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Recent advent of Aqueous-Two-Phase-System (ATPS), more biologically friendly compared to conventional oil-water systems, has shown great potential to rapidly generate aqueous droplets without tedious post-processing. However, understanding of underlying physics of droplet formation in ATPS is still in its infancy. In this paper, we investigate hydrodynamic behaviors and mechanisms of all-aqueous droplet formation in two flow-focusing droplet generators. Two incompatible polymers namely polyethylene glycol (PEG) and dextran (DEX) are mixed in water to make ATPS. The influence of inlet pressures and flow-focusing configurations on droplet sizes, and thread breakup length is studied. Flow regime mapping for two different configurations of droplet generators possessing junction angles of 30° and 90° is also obtained. The results show that droplet size is very susceptible to the junction angle while inlet pressures of the PEG and DEX flows readily control four main flow regimes including back flow, dripping, jetting and stratified.
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WANG, WENJUAN, XIAOQING ZHANG, TAO XIE, MEI LI, and ANPING LIAO. "STUDY ON THE SEPARATION OF BROMELAIN USING AQUEOUS TWO-PHASE EXTRACTION." In Proceedings of the 4th International Conference. WORLD SCIENTIFIC, 2004. http://dx.doi.org/10.1142/9789812702623_0073.

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WU, Jiang. "Separation of Glycyrrhizic Acid Using Smart Polymer Based Aqueous Two-Phase Systems." In 2018 International Conference on Energy Development and Environmental Protection (EDEP 2018). Atlantis Press, 2018. http://dx.doi.org/10.2991/edep-18.2018.39.

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Chen, Chia-Yuan, Wei-Feng Fang, Chiko Chen, Jing-Tang Yang, and Ping-Chiang Lyu. "Separation of Amino Acids by Aqueous Two-Phase Electrophoresis on the Micro-Pillar Chips." In 2006 1st IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE, 2006. http://dx.doi.org/10.1109/nems.2006.334830.

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Rodriguez, G. Youssef, C. Sacardo da Silva, C. Osamu Hokka, and M. Barboza. "Extraction of clavulanic acid in aqueous two-phase systems followed by separation through ultrafiltration." In Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009). WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814322119_0127.

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Xiaohong Zhao, Xiaohui Xu, Yongsheng Yan, Cuilan Yu, and Shiping Hu. "Preconcentration and separation of trace roxithromycin by [Bmim]BF4 - Na2CO3 Aqueous two-phase flotation system." In 2011 International Symposium on Water Resource and Environmental Protection (ISWREP). IEEE, 2011. http://dx.doi.org/10.1109/iswrep.2011.5893486.

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KIM, HONGHYUN, YEOUNSOO KIM, KWINAM PARK, SEOK-HEUNG JANG, SEOUNMOON LEE, and JINWON PARK. "THE SEPARATION OF MODIFIED CELLULASE WITH POLYALKYLENE OXIEDE DERIVATE USING REACTIVE AQUEOUS TWO PHASE SYSTEM." In Proceedings of the 4th International Conference. WORLD SCIENTIFIC, 2004. http://dx.doi.org/10.1142/9789812702623_0148.

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Masu, Smaranda, Viorica Dalea, and V. Cocheci. "THE APPLICATION OF LIQUID MEMBRANE PROCESSES IN WASTEWATER TECHNOLOGY. PART 2. SEPARATION, CONCENTRATION AND THE SELECTIVE RECOVERY OF PHENOL FROM DILUTED AQUEOUS SOLUTIONS AND WASTEWATERS." In International Symposium on Liquid-Liquid Two Phase Flow and Transport Phenomena. Begellhouse, 1997. http://dx.doi.org/10.1615/ichmt.1997.intsymliqtwophaseflowtranspphen.270.

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Dalea, Viorica, Smaranda Masu, and V. Cocheci. "THE APPLICATION OF LIQUID MEMBRANE PROCESSES IN WASTEWATER TECHNOLOGY. PART 1. SEPARATION, CONCENTRATION AND THE SELECTIVE RECOVERY OF COPPER IONS FROM DILUTE AQUEOUS SOLUTIONS AND WASTEWATERS." In International Symposium on Liquid-Liquid Two Phase Flow and Transport Phenomena. Begellhouse, 1997. http://dx.doi.org/10.1615/ichmt.1997.intsymliqtwophaseflowtranspphen.260.

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Reports on the topic "Aqueous two phase separation (ATPS)"

1

Harris, J. M. International Conference on Partitioning in Aqueous Two-Phase Systems: Advances in Separation in Biochenistry, Cell Biology and Biotechnology (7th) Held in New Orleans, Louisiana on 2-7 June 1991. Defense Technical Information Center, 1991. http://dx.doi.org/10.21236/ada250766.

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Crouch, Rebecca, Jared Smith, Bobbi Stromer, et al. Methods for simultaneous determination of legacy and insensitive munition (IM) constituents in aqueous, soil/sediment, and tissue matrices. Engineer Research and Development Center (U.S.), 2021. http://dx.doi.org/10.21079/11681/41720.

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Currently, no standard method exists for analyzing insensitive munition (IM) compounds in environmental matrices, with or without concurrent legacy munition compounds, resulting in potentially inaccurate determinations. The primary objective of this work was to develop new methods of extraction, pre-concentration, and analytical separation/quantitation of 17 legacy munition compounds along with several additional IM compounds, IM breakdown products, and other munition compounds that are not currently included in U.S. Environmental Protection Agency (EPA) Method 8330B. Analytical methods were developed to enable sensitive, simultaneous detection and quantitation of the 24 IM and legacy compounds, including two orthogonal high-performance liquid chromatography (HPLC) column separations with either ultraviolet (UV) or mass spectrometric (MS) detection. Procedures were developed for simultaneous extraction of all 24 analytes and two surrogates (1,2-dinitrobenzene, 1,2-DNB; o-NBA) from high- and low-level aqueous matrices and solid matrices, using acidification, solid phase extraction (SPE), or solvent extraction (SE), respectively. The majority of compounds were recovered from four tissue types within current limits for solids, with generally low recovery only for Tetryl (from 4 to 62%). A preparatory chromatographic interference removal procedure was adapted for tissue extracts, as various analytical interferences were observed for all studied tissue types.
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Crouch, Rebecca, Jared Smith, Bobbi Stromer, et al. Preparative, extraction, and analytical methods for simultaneous determination of legacy and insensitive munition (IM) constituents in aqueous, soil or sediment, and tissue matrices. Engineer Research and Development Center (U.S.), 2021. http://dx.doi.org/10.21079/11681/41480.

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No standard method exists for determining levels of insensitive munition (IM) compounds in environmental matrices. This project resulted in new methods of extraction, analytical separation and quantitation of 17 legacy and 7 IM compounds, daughter products of IM, and other munition compounds absent from USEPA Method 8330B. Extraction methods were developed for aqueous (direct-injection and solid-phase extraction [SPE]), soil, sediment, and tissue samples using laboratory-spiked samples. Aqueous methods were tested on 5 water sources, with 23 of 24 compounds recovered within DoD QSM Ver5.2 limits. New solvent extraction (SE) methods enabled recovery of all 24 compounds from 6 soils within QSM limits, and a majority of the 24 compounds were recovered at acceptable levels from 4 tissues types. A modified chromatographic treatment method removed analytical interferences from tissue extracts. Two orthogonal high-performance liquid chromatography-ultraviolet (HPLC-UV) separation methods, along with an HPLC–mass spectrometric (HPLC-MS) method, were developed. Implementing these new methods should reduce labor and supply costs by approximately 50%, requiring a single extraction and sample preparation, and 2 analyses rather than 4. These new methods will support environmental monitoring of IM and facilitate execution of risk-related studies to determine long-term effects of IM compounds.
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