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Relevant bibliographies by topics / Aqueous two phase separation (ATPS) / Dissertations / Theses

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Dissertations / Theses on the topic 'Aqueous two phase separation (ATPS)'

Author: Grafiati

Published: 9 September 2021

Last updated: 29 July 2025

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1

Balasubramaniam, Deepa. "Lysozyme Separation from Tobacco Extract by Aqueous Two-Phase Extraction." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/31272.

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Tobacco has long been considered as a host to produce large quantities of high-valued recombinant proteins. However, dealing with large quantities of biomass with a dilute concentration of product is a challenge for down-stream processing. Aqueous two-phase extraction (ATPE) has been used in purifying proteins from various sources. It is a protein-friendly process and can be scaled up easily. ATPE was studied for its applicability to recombinant protein purification from tobacco using egg white lysozyme as the model protein. Separate experiments with polyethyleneglycol(PEG)/salt/tobacco extra

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2

Periyannan, Rajeswari Prem Kumar. "Microfluidic aqueous two-phase system for continous partitioning of bacteria." Thesis, KTH, Skolan för bioteknologi (BIO), 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-149277.

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3

Prinz, Axel [Verfasser]. "Enzyme Separation Using Aqueous Two-Phase Extraction: Experiment, Model and Simulation / Axel Prinz." München : Verlag Dr. Hut, 2014. http://d-nb.info/1053859686/34.

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4

Hodgson, Christopher John. "Partitioning and separation of tPA (tissue plasminogen activator) in aqueous two-phase systems." Thesis, University of Reading, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334032.

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5

Ross, Kristin Coby. "Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction". Thesis, Virginia Tech, 2008. http://hdl.handle.net/10919/43471.

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Biopharmaceutical manufacturing is a rigorous and expensive process. Due to the medicinal nature of the product, a high purity level is required and several expensive purification steps must be utilized. Cost-effective production and purification is essential for any biopharmaceutical product to be successful and development of the fastest, most economical, and highest-yielding purification scheme is a constant engineering challenge. Commercial-scale purification schemes currently revolve around the use of multiple chromatography steps for the purification of biopharmaceutical products. Chroma

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6

Huaman?, Edgard Ronny Delgado. "Estudo da parti??o de Carbon Dots em sistemas aquosos bif?sicos: potencialidades para cat?lise de transfer?ncia de fase." UFVJM, 2017. http://acervo.ufvjm.edu.br/jspui/handle/1/1562.

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Data de aprova??o retirada da vers?o impressa do trabalho.<br>Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2018-01-04T19:50:32Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) edgard_ronny_delgado_huamani.pdf: 5898825 bytes, checksum: 78eee370de5fed297b3181e82d98f63d (MD5)<br>Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-01-17T18:40:25Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) edgard_ronny_delgado_huamani.pdf: 5898825 bytes,

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7

Huang, Ri-Bo. "Selective separation of yeast proteins by differential product release, enzymitic lysis and aqueous two-phase systems." Thesis, University of Reading, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280269.

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8

Laurence, M. B. "Separation of insoluble biological material downstream from a two liquid (organic/aqueous) phase whole cell biotransformation reactor." Thesis, University College London (University of London), 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.508462.

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9

Besel, Elif. "Use Of Triton X-114 Aqueous Two Phase System For Recovery Of Mushroom (agaricus Bisporus) Polyphenoloxidase." Master's thesis, METU, 2003. http://etd.lib.metu.edu.tr/upload/1262971/index.pdf.

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Mushroom (Agaricus bisporus) polyphenoloxidase (PPO) (EC 1.14.18.1) was isolated and purified using an aqueous two phase system composed of octyl phenol (ethyleneglycol) 7-8 ether (Triton X-114/TX-114). TX-114 is a non-ionic surfactant which thermoseparates in water and forms an aqueous two phase system with a surfactant-depleted top phase and a surfactant-enriched bottom phase. Critical micelle concentration (CMC) and cloud point of the surfactant are 0.17 mM and 22&ordm<br>C respectively. The partitioning behavior of mushroom PPO in water/TX-114 aqueous two-phase systems was studied and the

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10

Ferreira, Ana Maria da Conceição. "Separation of dyes with reversible aqueous biphasic systems." Master's thesis, Universidade de Aveiro, 2013. http://hdl.handle.net/10773/12572.

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Mestrado em Engenharia Química<br>The main objective of this work conveys on the study of reversible aqueous two-phase systems (ATPS), constituted by ionic liquids (ILs), and their potential application for the selective separation of dyes mainly used in the textile industry. The textile manufacturing is one of the main industries which discharges a heavy load of chemicals, especially large contents of dyes during the dying process through wastewaters, which results in severe environmental and economic concerns. In this context, this work focuses on the applicability of reversible ATPS, as wel

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11

Zimmermann, Sarah [Verfasser], and J. [Akademischer Betreuer] Hubbuch. "Cell Separation in Aqueous Two-Phase Systems - Development of an Integrated High-Throughput Screening Platform for Downstream Process Development for Cell-Based Products / Sarah Zimmermann ; Betreuer: J. Hubbuch." Karlsruhe : KIT-Bibliothek, 2017. http://d-nb.info/1130246558/34.

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12

Dhadge, Vijaykumar Laxman. "Magnetic purification of antibodies." Doctoral thesis, 2016. http://hdl.handle.net/10362/19003.

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This work aimed at the development of magnetic nanoparticles for antibody purification and at the evaluation of their performance in Magnetic fishing and in a newly developed hybrid technology Magnetic Aqueous Two Phase Systems. Magnetic materials were produced by coprecipitation and solvothermal approaches. Natural polymers such as dextran, extracellular polysaccharide and gum Arabic were employed for coating of iron oxide magnetic supports. Polymer coated magnetic supports were then modified with synthetic antibody specific ligands,namely boronic acid, a triazine ligand (named 22/8) and an U

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13

Wu, Wen-Chi, and 吳雯琪. "Optimization of Bromelain separation by using Aqueous Two-Phase System." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/29479168184294823024.

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碩士<br>元智大學<br>化學工程與材料科學學系<br>104<br>Aqueous two-phase system (ATPS) has been widely applied in various separation processes of biomaterials. ATPS possesses several advantages, such as low interfacial tension, good biocompatibility and resulting in high yield of target product. The partition behaviour of a target product in an ATPS could be affected by tie-line length (TLL), centrifugation speed, operational temperature, ionic strength, pH and volume ratio (VR) of the ATPS. In this study, recovery of expressed protease from flesh pineapple fruit using polymer/salt ATPS was investigated. The par

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14

Chang, Kai-Lun, and 張凱綸. "Separation of aqueous two-phase systems for the unsaturated antioxidant polyphenols." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/76526514524074352031.

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碩士<br>元智大學<br>化學工程與材料科學學系<br>105<br>Aqueous two phase system (ATPS) is a liquid- liquid extraction in two-phases present aqueous solution. Two hydrophilic substances are mixed at rarious concentrations and then the aqueous two phase system was formed spontaneously. The extraction of two phase system could be operated in the mild condition with large capacity, and the process could be scaled up easily. Aqueous two phase system contains polyethlene glycol and inorganic salt (K2HPO4/KH2PO4) was chosen for feasibility study. This study investigated phase equilibrium of the system and evaluated opt

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15

Ming, Chen Jain, and 陳建銘. "Fermentation and Separation of the Chitinase in Aqueous Two-Phase System." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/58342858057821771252.

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碩士<br>長庚大學<br>化學工程研究所<br>90<br>Fermentation was carried out in PEG 20,000 6.0% and PES 100 10.0%、EO-PO copolymer 8.6% (w/w) and PES 200 10.0% (w/w)aqueous two-phase systems (ATPS) to produce chitinase by Serratia marcescens. In PEG 20,000 6% (w/w) and PES 100 10.0% (w/w) ATPS, the bacteria tended to partition mainly to the interface and bottom phase, so that the enzyme was partition to the top phase. In this system, the enzyme partition coefficient is 1.27、volume ratio (Vb/Vt) is 0.25 and top phase enzyme yield is 83.5%. In EO-PO copolymer 8.6% (w/w) and PES 200 10.0% (w

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16

Wu, Ming-chien, and 吳明鍵. "The Extraction Separation of Lipase by Triblock Copolymer Aqueous Two-Phase System." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/31334374039702628844.

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碩士<br>國立成功大學<br>化學工程學系<br>86<br>It is important to maintain the activity of enzyme and reduce the cost of purification. In this study, enzymes such as C. rugosa lipase and A. radioresistens lipase were extracted by using aqueous two-phase extraction system. It was carried out by using triblock copolymer as one phase of the aqueous two-phase system. High selectivity and high productivity can be theadvantages from the aqueous two-phase extraction system. Acrylic polyampholyteshave isoelectric

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17

LAI, JIE-YONG, and 賴傑勇. "Application of aqueous two-phase partition chromatography to separation and purification of enzyme." Thesis, 1989. http://ndltd.ncl.edu.tw/handle/49547195234807262287.

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18

Chen, Chia-Yuan, and 陳嘉元. "Separation of amino acids by aqueous two-phase electrophoresis on the micropillars chips." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/33362066496844306433.

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19

TSENG, C. W., та 曾靖雯. "The Extraction Separation of α-Amylase by Triblock Copolymer Aqueous Two-Phase System". Thesis, 2000. http://ndltd.ncl.edu.tw/handle/51450941749332447470.

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碩士<br>國立成功大學<br>化學工程學系<br>87<br>It is important to maintain the activity of enzyme and reduce the cost of purification. In this study, enzymes such as Bacillus licheniformis α-amylase and Bacillus amyloliquefanciens a-amylase were extracted by using aqueous two-phase extraction system. It was carried out by using triblock copolymer as one phase of the aqueous two-phase system. High selectivity and high productivity are the advantages of the aqueous two-phase extraction system. Acrylic polyampholytes have isoelectric points at which the polyelectrolyte can precipitate. Hence, it can be recyc

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20

LIN, PO-TS'UN, and 林柏村. "Separation of mammal cells and purification of Cephalosporin C by Aqueous two phase and Counter Current Chromatography." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/81406967340422344829.

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21

Yang, Wu-Yung, and 楊武勇. "Extraction of Cephalosporin C from Whole Broth and Separation of Desacetyl Cephalosporin C by Aqueous Two Phase Partition." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/13348546118231388595.

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