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Journal articles on the topic 'Aqueous two phase separation (ATPS)'

Author: Grafiati

Published: 9 September 2021

Last updated: 1 February 2022

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1

Kruse, Thomas, Axel Schmidt, Markus Kampmann, and Jochen Strube. "Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems." Antibodies 8, no. 3 (2019): 40. http://dx.doi.org/10.3390/antib8030040.

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Therapeutic monoclonal antibodies (mAb) are used for the treatment of numerous serious diseases, which have led to an increasing demand over the last decades. Increased cell density and mAb titer of the cultivation broth lead to great challenges for the subsequent clarification and capture operations in the downstream process. As an alternative approach to the conventional downstream process, a selective mAb extraction via an aqueous two-phase system (ATPS) directly from the cultivation broth of a mAb producing industrial relevant chinese hamster ovary (CHO) cell line was investigated. An effi

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2

Ma, Chao, Xiao Dong Chen, Yin Qiu Kong, and Li Ming Che. "Phase Diagram of Aqueous Two-Phase System (ATPS) Composed of Polyethylene Glycol (PEG) and Gelatin." Advanced Materials Research 554-556 (July 2012): 286–94. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.286.

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Phase diagram of aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and gelatin is of paramount importance for the application of such system in microencapsulation of bioactive compounds. Phase separation of PEG/gelatin aqueous solution was investigated in the present work and the phase diagram of resultant ATPS was reported for the first time. The results show that phase separation will happen if the solid content of PEG/gelatin aqueous solution is higher than a critical value, resulting in an ATPS. The resultant ATPS consists of a low-density phase enriched in PEG and a de

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3

Rogers, Bradley A., Kelvin B. Rembert, Matthew F. Poyton, et al. "A stepwise mechanism for aqueous two-phase system formation in concentrated antibody solutions." Proceedings of the National Academy of Sciences 116, no. 32 (2019): 15784–91. http://dx.doi.org/10.1073/pnas.1900886116.

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Aqueous two-phase system (ATPS) formation is the macroscopic completion of liquid–liquid phase separation (LLPS), a process by which aqueous solutions demix into 2 distinct phases. We report the temperature-dependent kinetics of ATPS formation for solutions containing a monoclonal antibody and polyethylene glycol. Measurements are made by capturing dark-field images of protein-rich droplet suspensions as a function of time along a linear temperature gradient. The rate constants for ATPS formation fall into 3 kinetically distinct categories that are directly visualized along the temperature gra

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4

Atefi, Ehsan, Ramila Joshi, and Hossein Tavana. "Effect of Molecular Weight of Phase Polymers on Partition of Cells in Aqueous Two-Phase Systems." MRS Advances 2, no. 45 (2017): 2415–26. http://dx.doi.org/10.1557/adv.2017.425.

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ABSTRACTDue to their aqueous environment and biocompatible polymers, aqueous two-phase systems (ATPS) provide a mild environment for partition of cells. A comprehensive understanding of cell partition in ATPS will facilitate cell separation and fractionation in ATPS for downstream cell-based analytical applications and various cellular and molecular biology studies. We report the effect of molecular weight of phase polymers on partition of cells between two aqueous phases and their interface. We generate three different ATPS by dissolving polyethylene glycol and dextran of different molecular

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Pereira, David Y., Chloe M. Wu, So Youn Lee, Eumene Lee, Benjamin M. Wu, and Daniel T. Kamei. "Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media." SLAS TECHNOLOGY: Translating Life Sciences Innovation 24, no. 5 (2019): 515–26. http://dx.doi.org/10.1177/2472630319861369.

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In previous work, our group discovered a phenomenon in which a mixed polymer–salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper. While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components. However, since the mechanism of phase separation in porous media is not com

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Souza, Ranyere L., Sónia P. M. Ventura, Cleide M. F. Soares, João A. P. Coutinho, and Álvaro S. Lima. "Lipase purification using ionic liquids as adjuvants in aqueous two-phase systems." Green Chemistry 17, no. 5 (2015): 3026–34. http://dx.doi.org/10.1039/c5gc00262a.

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7

Liu, Shao Peng, Zhi Min Zong, Shan Shan Zhang, et al. "Study of Aqueous Two Phase System on Water Soluble Bio Oil." Advanced Materials Research 236-238 (May 2011): 49–53. http://dx.doi.org/10.4028/www.scientific.net/amr.236-238.49.

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This paper was aimed to study the aqueous two phase extraction (ATPE) on water soluble bio oil(SBO), in which ethanol, K2HPO4, NaH2PO4and (NH4)2SO4were selected to form the water soluble bio oil(SBO) aqueous two phase system (ATPS). After phase separation, the extraction rates and the volume ratios of the upper to lower layer of the ATPSs were investigeted. Both the upper and lower layers of these SBO ATPSs were analyzed by elemental analyzer and Fourier transformation infrared spectrometer (FTIR). The different extraction rates indicates the influnces of different salt during the phase sapera

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Dembczyński, Radosław, Wojciech Białas, and Tomasz Jankowski. "Determination of phase diagrams and thermoseparation behaviour of aqueous two-phase systems composed of ethylene oxide–propylene oxide random copolymer and potassium phosphate." Chemical and Process Engineering 33, no. 3 (2012): 411–29. http://dx.doi.org/10.2478/v10176-012-0036-8.

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Abstract The application of aqueous two-phase systems (ATPS) is a cost-effective and simple method of protein separation (including enzymes) from complex systems. The first stage of designing the protein purification process in an ATPS involves the identification of the conditions for the formation of a given extraction system. For this purpose, the conditions for the formation of ATPSs in a thermoseparating EO50PO50 polymer/potassium phosphates system have been studied. Factors determining the ATPS formation comprised: separation temperature (4ºC or 20ºC), phosphate solution pH (6, 7.5 or 9)

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9

Seo, Hanjin, Changwoo Nam, Eunseo Kim, Juhyun Son, and Hyomin Lee. "Aqueous Two-Phase System (ATPS)-Based Polymersomes for Particle Isolation and Separation." ACS Applied Materials & Interfaces 12, no. 49 (2020): 55467–75. http://dx.doi.org/10.1021/acsami.0c16968.

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10

Wu, Jiang, Ying Kai Tong, and Li Tong Ban. "Separation of Casein Phosphopeptides (a Functional Food Material) by Aqueous Two-Phase Systems." Advanced Materials Research 554-556 (July 2012): 1511–14. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.1511.

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PEG 10000 / (NH4)2SO4 ATPS was applied to extract casein phosphopeptides. By studying on the partitioning behavior of CPPs in this system, it is concluded that the purification fold of CPPs could reach 7.09 when the system consisted of 20% PEG 10000 and 10% (NH4)2SO4.

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Wang, Wei Jie, Xiao Le Zhang, Chang Bo Xu, and Hong Yan Cheng. "Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System." Applied Mechanics and Materials 138-139 (November 2011): 995–1001. http://dx.doi.org/10.4028/www.scientific.net/amm.138-139.995.

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In this article, the partitioning behavior of C-phycocyanin (C-PC) in aqueous two-phase systems (ATPS) with different component was investigated to evaluate the applicability of ATPS for extracting high purity of C-PC without chromatography. Under the optimized conditions (pH = 6; PEG-2000, 16 %, w/w; potassium sodium tartrate 21 %, w/w), a maximum purity of 5.01 was achieved after the whole separation process and the yield of the C-PC was 76.94 %.

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Wang, Yun, Juan Han, Xue-qiao Xie, and Chun-xiang Li. "Extraction of trace acetylspiramycin in real aqueous environments using aqueous two-phase system of ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate and phosphate." Open Chemistry 8, no. 6 (2010): 1185–91. http://dx.doi.org/10.2478/s11532-010-0080-5.

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AbstractThe partitioning of acetylspiramycin was carried out in an aqueous two-phase system (ATPS) formed by a hydrophilic ionic liquid (1-butyl-3-methylimidazolium tetrafluoraborate, [Bmim]BF4) and NaH2PO4. This ATPS is a simple, non-toxic and effective sample pretreatment technique, which was developed for the simultaneous separation, enrichment and rapid analysis of acetylspiramycin coupled with molecular fluorescence spectrophotometry. Analysis of the liquid-liquid equilibrium of [Bmim]BF4-salt ATPS demonstrated that the salting-out ability of different salts may be related to the Gibbs en

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Kresheck, Gordon C., and Zheng Wang. "A new micellar aqueous two-phase partitioning system (ATPS) for the separation of proteins." Journal of Chromatography B 858, no. 1-2 (2007): 247–53. http://dx.doi.org/10.1016/j.jchromb.2007.08.037.

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14

Jugler, Collin, Jussi Joensuu, and Qiang Chen. "Hydrophobin-Protein A Fusion Protein Produced in Plants Efficiently Purified an Anti-West Nile Virus Monoclonal Antibody from Plant Extracts via Aqueous Two-Phase Separation." International Journal of Molecular Sciences 21, no. 6 (2020): 2140. http://dx.doi.org/10.3390/ijms21062140.

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The development of monoclonal antibodies (mAbs) has provided vast opportunities to treat a wide range of diseases from cancer to viral infections. While plant-based production of mAbs has effectively lowered the upstream cost of mAb production compared to mammalian cell cultures, further optimization of downstream processing, especially in extending the longevity of Protein A resin by an effective bulk separation step, will further reduce the overall prohibitive cost of mAb production. In this study, we explored the feasibility of using aqueous two-phase separation (ATPS) in capturing and sepa

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15

Jiang, Bin, Jiaxin Na, Lele Wang, Dongmei Li, Chunhong Liu, and Zhibiao Feng. "Separation and Enrichment of Antioxidant Peptides from Whey Protein Isolate Hydrolysate by Aqueous Two-Phase Extraction and Aqueous Two-Phase Flotation." Foods 8, no. 1 (2019): 34. http://dx.doi.org/10.3390/foods8010034.

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At present, peptides are separated by molecular exclusion chromatography and liquid chromatography. A separation method is needed in any case, which can be scaled up for industrial scale. In this study, aqueous two-phase extraction (ATPE) and aqueous two-phase flotation (ATPF) were applied to separate and enrich antioxidant peptides from trypsin hydrolysates of whey protein isolates (WPI). The best experimental conditions were investigated, and the results were evaluated using the 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radical scavenging activity of the peptides-per-uni

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Sun, Danyu, Runping Wang, Fenfang Li, Lianglei Liu, and Zhijian Tan. "Enantioselective Extraction of Phenylalanine Enantiomers Using Environmentally Friendly Aqueous Two-Phase Systems." Processes 6, no. 11 (2018): 212. http://dx.doi.org/10.3390/pr6110212.

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(1) Background: The environmentally friendly choline-amino acid ionic liquids (ChAAILs) and deep eutectic solvents (DESs) have been used as excellent alternatives to volatile organic compounds (VOCs) and ionic liquids (ILs) in recent years; (2) Methods: Thus, ChAAILs/salt and DESs/salt aqueous two-phase systems (ATPSs) were developed for the chiral extraction of phenylalanine enantiomers. The optimum ATPS of [Ch][L-Pro]/K3PO4 was chosen, and the influencing parameters were investigated, including ChAAILs concentration, salt concentration, chiral selector concentration, extraction temperature,

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Pericin, Draginja, Senka Madjarev-Popovic, and Zuzana Vastag. "Partitioning of acid protease from Penicillium roqueforti in aqueous two-phase system polyethylene glicol/phosphate." Acta Periodica Technologica, no. 39 (2008): 171–80. http://dx.doi.org/10.2298/apt0839171p.

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The partitioning of acid protease produced by submerged fermentation of Penicillium roqueforti in the aqueous two-phase system polyethylene glycol/sodium phosphate was studied. In the system with 15% polyethylene glycol 4000/25% NaH2PO4 favourable partition coefficient and the top phase yield were obtained, 2.651 and 56.51%, respectively. The purification of acid protease from crude enzyme was optimized by optimizing separation factor. Completely randomized factorial design was applied and the system 15.54% PEG 4000/20% NaH2PO4 appeared to be optimal. Statistically calculated separation factor

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18

Ahmed, Tasdiq, Cameron Yamanishi, Taisuke Kojima, and Shuichi Takayama. "Aqueous Two-Phase Systems and Microfluidics for Microscale Assays and Analytical Measurements." Annual Review of Analytical Chemistry 14, no. 1 (2021): 231–55. http://dx.doi.org/10.1146/annurev-anchem-091520-101759.

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Phase separation is a common occurrence in nature. Synthetic and natural polymers, salts, ionic liquids, surfactants, and biomacromolecules phase separate in water, resulting in an aqueous two-phase system (ATPS). This review discusses the properties, handling, and uses of ATPSs. These systems have been used for protein, nucleic acid, virus, and cell purification and have in recent years found new uses for small organics, polysaccharides, extracellular vesicles, and biopharmaceuticals. Analytical biochemistry applications such as quantifying protein–protein binding, probing for conformational

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19

Jiang, Bin, Jiaxin Na, Lele Wang, Dongmei Li, Chunhong Liu, and Zhibiao Feng. "Eco-Innovation in Reusing Food By-Products: Separation of Ovalbumin from Salted Egg White Using Aqueous Two-Phase System of PEG 1000/(NH4)2SO4." Polymers 11, no. 2 (2019): 238. http://dx.doi.org/10.3390/polym11020238.

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For the purpose of reducing pollution and the rational use of salted egg white, which is a byproduct of the manufacturing process of salted egg yolk, an aqueous two-phase system (ATPS) composed of polyethylene glycols (PEG 1000) and (NH4)2SO4 was investigated to selectively separate ovalbumin (OVA) from salted egg white. With the aim of optimizing the selective separation of OVA using ATPS, a response surface method (RSM) experiment was carried out on the basis of a single-factor experiment. The OVA was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE), reve

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Wu, Jiang, Li Tong Ban, and Yu Wang. "Separation of Curcumine (a Functional Food Colourant Material) from Rhizoma curcuma longae by Aqueous Two-Phase Systems." Applied Mechanics and Materials 217-219 (November 2012): 961–64. http://dx.doi.org/10.4028/www.scientific.net/amm.217-219.961.

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The objective of this paper was to separate curcumine from Rhizoma curcuma longae and aqueous two-phase extraction technology was employed to perform the study. Four types of PEG (400, 600, 2000 and 4000) and three types of salt ((NH4)2SO4, KH2PO4 and K2HPO4) were tested to form the aqueous two-phase systems. The results showed that the ATPS composed of PEG 400 and (NH4)2SO4 were the best. And the partition coefficient of curcumine could reach 21.79 when the concentration of PEG 400 and (NH4)2SO4 was 14.6% and 26% respectively.

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Khan, Bilal Muhammad, Kit-Leong Cheong, and Yang Liu. "ATPS: “Aqueous two-phase system” as the “answer to protein separation” for protein-processing food industry." Critical Reviews in Food Science and Nutrition 59, no. 19 (2018): 3165–78. http://dx.doi.org/10.1080/10408398.2018.1486283.

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Duman, Yonca Avcı, Arda Acemi, Yonca Yuzugullu, and Fazıl Özen. "Separation of catalase from Amsonia orientalis with single step by aqueous two-phase partitioning system (ATPS)." Separation Science and Technology 52, no. 4 (2016): 691–99. http://dx.doi.org/10.1080/01496395.2016.1253588.

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23

Zhao, Wen Yan, Jing Zhang, Xiang Ge, Yue Lu, and Rui Sheng Hu. "Extraction of Gatifloxacin in Water by Use of Ionic Liquid–Salt Aqueous Two-Phase System." Advanced Materials Research 356-360 (October 2011): 195–98. http://dx.doi.org/10.4028/www.scientific.net/amr.356-360.195.

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An ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate( [BMIM] BF4) / salt aqueous two-phase systems (ATPS) was presented as a simple, rapid and effective sample pretreatment technique coupled with double-beam UV-Vis spectrophotometer for analysis of gatifloxacin in water. For the purpose to choose optimal conditions, various factors were considered systematically, and the results indicated that the pH value, the salt-out ability of salt and the amount of [BMIM] BF4 had great inﬂuence on phase separation and extraction. Under the optimal condition, gatifloxacin in different concentrati

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Yang, Yu, Bilal Muhammad Khan, Xiping Zhang, Yongjie Zhao, Kit-Leong Cheong, and Yang Liu. "Advances in Separation and Purification of Bioactive Polysaccharides through High-speed Counter-Current Chromatography." Journal of Chromatographic Science 58, no. 10 (2020): 992–1000. http://dx.doi.org/10.1093/chromsci/bmaa063.

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Abstract Polysaccharides, with an extensive distribution in natural products, represent a group of natural bioactive substances having widespread applications in health-care food products and as biomaterials. Devising an efficient system for the separation and purification of polysaccharides from natural sources, hence, is of utmost importance in the widespread applicability and feasibility of research for the development of polysaccharide-based products. High-speed counter-current chromatography (HSCCC) is a continuous liquid–liquid partitioning chromatography with the ability to support a hi

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Ni, Yunzhou, Zhixiang Qian, Yu Yin, Weien Yuan, Fei Wu, and Tuo Jin. "Polyvinyl Alcohol/Chitosan/Polyhexamethylene Biguanide Phase Separation System: A Potential Topical Antibacterial Formulation with Enhanced Antimicrobial Effect." Molecules 25, no. 6 (2020): 1334. http://dx.doi.org/10.3390/molecules25061334.

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An aqueous polyvinyl alcohol (PVA)/chitosan (CHT)/polyhexamethylene biguanide (PHMB) blends (PVA/CHT/PHMB blends) has been developed as a potential low dose topical antibacterial formulation with enhanced antimicrobial effect. The preparation of PVA/CHT/PHMB blends was quite facilely, with just dissolved PVA, CHT, PHMB in water in order. There was the aggregates with 100 nm size around induced by phase separation in the blends and an aqueous two-phase system (ATPS) formed, as non-ionic polymer PVA formed a continuous phase and cationic polymer CHT and PHMB formed dispersed phases. The minimum

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Mařík, Karel, Linda Tichá, Lucie Vobecká, and Michal Přibyl. "Theoretical study on enzyme synthesis of cephalexin in a parallel-flow microreactor combined with electrically driven ATPS microextraction." Reaction Chemistry & Engineering 5, no. 3 (2020): 570–83. http://dx.doi.org/10.1039/c9re00482c.

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A mathematical model of a microfluidic device with two aqueous phases for the simultaneous cephalexin production and its separation from a reaction mixture was developed. The model anticipates the continuous cephalexin synthesis and enzyme recyclation.

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Valadares, Alberto, Clara França Valadares, Leandro Rodrigues de Lemos, Aparecida Barbosa Mageste, and Guilherme Dias Rodrigues. "Separation of cobalt and nickel in leach solutions of spent nickel-metal hydride batteries using aqueous two-phase systems (ATPS)." Hydrometallurgy 181 (November 2018): 180–88. http://dx.doi.org/10.1016/j.hydromet.2018.09.006.

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Dhadge, Vijaykumar L., Patricia I. Morgado, Filomena Freitas, et al. "An extracellular polymer at the interface of magnetic bioseparations." Journal of The Royal Society Interface 11, no. 100 (2014): 20140743. http://dx.doi.org/10.1098/rsif.2014.0743.

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FucoPol, a fucose-containing extracellular polysaccharide (EPS) produced by bacterium Enterobacter A47 using glycerol as the carbon source, was employed as a coating material for magnetic particles (MPs), which were subsequently functionalized with an artificial ligand for the capture of antibodies. The performance of the modified MPs (MP–EPS-22/8) for antibody purification was investigated using direct magnetic separation alone or combined with an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and dextran. In direct magnetic capturing, and using pure protein solutions o

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Voshkin, Andrey, Vitaliy Solov’ev, Mikhail Kostenko, Yulia Zakhodyaeva, and Oleg Pokrovskiy. "A Doubly Green Separation Process: Merging Aqueous Two-Phase Extraction and Supercritical Fluid Extraction." Processes 9, no. 4 (2021): 727. http://dx.doi.org/10.3390/pr9040727.

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Aqueous two-phase extraction (ATPE) is a green separation technique which uses mixtures of water and environmentally benign polymers such as polyethylene glycol (PEG) as solvents. One of the challenges in implementing this extraction on an industrial scale is finding a suitable method for the isolation of target compounds from water-polymer solutions after the extraction, without diminishing ecological benefits of the method. In this paper, we propose using another green separation technique, supercritical fluid extraction (SFE), for the back-extraction of low molecular weight medium polarity

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Jiang, Bin, Jiaxin Na, Lele Wang, Dongmei Li, Chunhong Liu, and Zhibiao Feng. "Reutilization of Food Waste: One-Step Extration, Purification and Characterization of Ovalbumin from Salted Egg White by Aqueous Two-Phase Flotation." Foods 8, no. 8 (2019): 286. http://dx.doi.org/10.3390/foods8080286.

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For the purpose of reducing pollution and the reutilization of salted egg whites, which are byproducts of the manufacturing process of salted egg yolks and normally treated as waste, an aqueous two-phase flotation (ATPF) composed of polyethylene glycols (PEG 1000) and (NH4)2SO4 was applied to develop a simple, inexpensive and efficient process for the separation of ovalbumin (OVA) from salted egg whites. The effects of the concentration of PEG, the concentration of (NH4)2SO4, the flow rate and the flotation time on the flotation efficiency (Y) and purity (P) of OVA were investigated. A respons

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Choi, Daeho, Eunjeong Lee, Sung-Jin Kim, and Minsub Han. "Passive droplet generation in aqueous two-phase systems with a variable-width microchannel." Soft Matter 15, no. 23 (2019): 4647–55. http://dx.doi.org/10.1039/c9sm00469f.

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Zhou, Chunmei, Pingan Zhu, Ye Tian, Xin Tang, Rui Shi, and Liqiu Wang. "Microfluidic generation of aqueous two-phase-system (ATPS) droplets by oil-droplet choppers." Lab on a Chip 17, no. 19 (2017): 3310–17. http://dx.doi.org/10.1039/c7lc00696a.

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Singh, Namita, and S. K. Mandal. "Studies on Nisin Production by Isolated Lactobacillus Species in Aqueous Two-phase System." Mapana - Journal of Sciences 6, no. 1 (2007): 69–80. http://dx.doi.org/10.12723/mjs.10.6.

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Isolated lactobacillus sp.(from cheese whey) was examined for its ability to produce bacteriocin like nisin in submerged fermentation and aqueous two-phase system(ATPS) at pH 6.5, 30.C for 18 h fermentation using M17S medium with 5%(v/v) inoculum and the nisin yields were obtained 4500 IU/ml and 20216 IU/ml where as biomass yield obtained 19.55 mg/ml and 31.88 mg/ml respectively.The maximum yield of nisin (24800 IU/ml) and biomass (22.49 mg/ml) were obtained in ATPS (28% PEG 6000 and 4% MgSO4.7H20 with 5-times concentrated M17 medium containing 0.5% lactose as carbon source) under similar cond

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Agarwal, Rishima, Kristin Robin Ko, Paul F. Gratzer, and John P. Frampton. "Biopatterning of Keratinocytes in Aqueous Two-Phase Systems as a Potential Tool for Skin Tissue Engineering." MRS Advances 2, no. 45 (2017): 2443–49. http://dx.doi.org/10.1557/adv.2017.357.

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ABSTRACTExtrusion-based bioprinting (EBP) is limited by loss of pattern fidelity when printing on wet substrates. This can be overcome using aqueous two-phase systems (ATPSs) as novel ink formulations for EBP. In this study, optimal concentrations of ATPS “inks” were determined and used to pattern human epidermal keratinocyte (HEK001) colonies on a wet substrate for promoting epidermal growth. Four equilibrated and non-equilibrated ATPS formulations were tested for stable ATPS formation and uniform cell patterning. We identified an optimal formulation that produced stable droplets on a standar

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Moon, Byeong-Ui, Steven G. Jones, Dae Kun Hwang, and Scott S. H. Tsai. "Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures." Lab on a Chip 15, no. 11 (2015): 2437–44. http://dx.doi.org/10.1039/c5lc00217f.

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Jiang, Bin, Zhi Biao Feng, Chun Hong Liu, Ying Cao Xu, Dong Mei Li, and Guo Ji. "Partitioning Behavior of Penicillin G in Aqueous Two Phase System Based on Ionic Liquids." Advanced Materials Research 864-867 (December 2013): 324–27. http://dx.doi.org/10.4028/www.scientific.net/amr.864-867.324.

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An aqueous two-phase system (ATPS) was presented with hydrophilic ionic liquid 1-Butyl-3-methylimid-azolium tetrafluoroborate ([BmiBF4) and salts for the extraction of penicillin G in this paper. The phase forming abilities of different salts were investigated to choose a suitable salt.The partitioning behavior of penicillin G in the ATPS was investigated.Concentrations of (NH4)2SO4, penicillin G, and [BmiBF4 were evaluated to determine their effects on the extraction yield of penicillin G. It was found that extraction yield strongly depended on the concentration of [BmiBF4, penicillin and (NH

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Shang, Yazhuo, Honglai Liu, Ying Hu, and John M. Prausnitz. "Aqueous Two‐Phase System (ATPS) Containing Gemini (12‐3‐12,2Br−) and SDS I: Phase Diagram and Properties of ATPS." Journal of Dispersion Science and Technology 27, no. 3 (2006): 335–39. http://dx.doi.org/10.1080/01932690500358105.

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Wang, Qing Yun, Qin Lu Lin, and Xiao Hao Li. "Extraction of Lipase from Rice Bran Using Aqueous Two-Phase System." Advanced Materials Research 651 (January 2013): 384–88. http://dx.doi.org/10.4028/www.scientific.net/amr.651.384.

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Aqueous two-phase system (ATPS) is an efficient and economical bioseparation technique. In this paper, the PEG2000/(NH4)2SO4 aqueous two-phase system was used to extract lipase from rice bran, and the best conditions were 24% PEG2000, 14% (NH4)2SO4, 15% addition of crude enzyme and pH 7.5. At this condition, the partition coefficient and activity recovery were highest and the activity recovery could achieve 90.29%.

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Dobreva, Valentina, Boriana Zhekova, and Georgi Dobrev. "Use of Aqueous Two-Phase and Three-Phase Partitioning Systems for Purification of Lipase Obtained in Solid-State Fermentation by Rhizopus arrhizus." Open Biotechnology Journal 13, no. 1 (2019): 27–36. http://dx.doi.org/10.2174/1874070701913010027.

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Background: Purification of enzymes by conventional methods such as precipitation and chromatographic techniques is a costly and time-consuming procedure and may lead to low yields of enzyme activity. Alternative liquid-liquid extraction methods such as Aqueous Two-Phase Systems (ATPS) and Three Phase Partitioning (TPP) are characterized by the high enzyme yields and purification degree. Objective: The objective of this study was the application of partitioning systems ATPS and TPP for purification of lipase produced in solid-state fermentation by Rhizopus arrhizus. Methods: ATPS and TPP were

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Tsumoto, Kanta, and Kenichi Yoshikawa. "The Aqueous Two Phase System (ATPS) Deserves Plausible Real-World Modeling for the Structure and Function of Living Cells." MRS Advances 2, no. 45 (2017): 2407–13. http://dx.doi.org/10.1557/adv.2017.358.

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ABSTRACTAn aqueous two phase system (ATPS) is composed of binary hydrophilic polymers, for example, polyethylene glycol (PEG) and dextran, under an immiscible condition, and can also exhibit micro-segregation to produce cell-sized microcompartments like water-in-water microdroplets. Without membranes, interestingly, the microdroplet can serve as a micro-vessel (reactor) that contains various biochemical macromolecules like DNAs and proteins. We here present that PEG/dextran ATPS micro-segregation can provide an effective soft boundary to separate these biochemical macromolecules from the exter

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NAKAGAWA, Tomohiro, Shunpei OHARA, Hidetoshi KOTERA, Hirofumi SHINTAKU, and Ryuji YOKOKAWA. "Patterning and evaluation of two motor proteins by Aqueous Two-Phase System (ATPS)." Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2018.30 (2018): 1G06. http://dx.doi.org/10.1299/jsmebio.2018.30.1g06.

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Xiang, Cheng, Jie Chang, Ying Ying Yue, Ju Wang, and Yan Fu. "The Application of Aqueous Two-phase System in the Extraction of Natural Products from Chinese Herbal Medicine: A Review." Current Organic Chemistry 23, no. 6 (2019): 704–20. http://dx.doi.org/10.2174/1385272823666190404163748.

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Background: In the past decades, Chinese herbal medicine has attracted worldwide attention because they contain a variety of active ingredients which are beneficial to human health. As a result, there is a growing interest in the extraction of these substances. However, traditional extraction methods not only need a large amount of extractant, but are also time-consuming, moreover, the extraction efficiency is extremely poor and tedious purification steps are required to purify the crude extract. Thus, researchers hope to find an alternative method for the extraction of these components and th

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Amid, Mehrnoush, Fara Syazana Murshid, Mohd Yazid Manap, and Muhaini Hussin. "A Novel Aqueous Micellar Two-Phase System Composed of Surfactant and Sorbitol for Purification of Pectinase Enzyme fromPsidium guajavaand Recycling Phase Components." BioMed Research International 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/815413.

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A novel aqueous two-phase system composed of a surfactant and sorbitol was employed for the first time to purify pectinase fromPsidium guajava. The influences of different parameters, including the type and concentration of the surfactant and the concentration and composition of the surfactant/sorbitol ratio, on the partitioning behavior and recovery of pectinase were investigated. Moreover, the effects of system pH and the crude load on purification fold and the yield of purified pectinase were studied. The experimental results indicated that the pectinase was partitioned into surfactant-rich

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Liu, Can, Shuochen Liu, Lingli Zhang, Xiaoxue Wang, and Lanqing Ma. "Partition Behavior in Aqueous Two-Phase System and Antioxidant Activity of Flavonoids from Ginkgo biloba." Applied Sciences 8, no. 11 (2018): 2058. http://dx.doi.org/10.3390/app8112058.

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In this work, the aqueous two-phase extraction parameters and in vitro antioxidant activity of total flavonoids from Ginkgo biloba were investigated. The partition behavior of the flavonoids in an aqueous two-phase system (ATPS) was analyzed, the extraction parameters were optimized using response surface methodology, and the antioxidant activity of the flavonoids was determined by free-radical scavenging tests (1,1-diphenyl-2-picrylhydrazyl (DPPH) assay) and the ferric reducing/antioxidant power (FRAP) assay. The results showed that the concentration of ammonium sulfate was negatively correla

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Moon, Byeong-Ui, Dae Kun Hwang, and Scott S. H. Tsai. "Shrinking, growing, and bursting: microfluidic equilibrium control of water-in-water droplets." Lab on a Chip 16, no. 14 (2016): 2601–8. http://dx.doi.org/10.1039/c6lc00576d.

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Hazra, S., K. S. Jayaprakash, K. Pandian, A. Raj, S. K. Mitra, and A. K. Sen. "Non-inertial lift induced migration for label-free sorting of cells in a co-flowing aqueous two-phase system." Analyst 144, no. 8 (2019): 2574–83. http://dx.doi.org/10.1039/c8an02267d.

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We present a novel label-free passive microfluidic technique for isolation of cancer cells (EpCAM+ and CD45−) from peripheral blood mononuclear cells (PBMCs) (CD45+ and EpCAM−) in aqueous two-phase system (ATPS).

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Ferreira, Graziela Batista, Alex Ferreira Evangelista, João Baptista Severo Junior, et al. "Partitioning optimization of proteins from Zea mays malt in ATPS PEG 6000/CaCl2." Brazilian Archives of Biology and Technology 50, no. 3 (2007): 557–64. http://dx.doi.org/10.1590/s1516-89132007000300020.

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This work aimed to establish the relationship between the compositions and pH of ATPS PEG 6000/CaCl2 and the proteins partition from maize malt and also to simplify the process optimization in ATPS for a statistical model, established by response surface methodology (RSM). Results showed that these were no influence of pH on the phase diagrams and on the composition of tie line length of PEG 6000/CaCl2 ATPS. SRM analyses showed that elevated pH and larger tie line length were the best conditions for recovering of maize malt proteins. The maximum partition coefficient by PEG 6000/CaCl2 ATPS was

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Simon, Arlyne B., John P. Frampton, Nien-Tsu Huang, Katsuo Kurabayashi, Sophie Paczesny, and Shuichi Takayama. "Aqueous two-phase systems enable multiplexing of homogeneous immunoassays." TECHNOLOGY 02, no. 02 (2014): 176–84. http://dx.doi.org/10.1142/s2339547814500150.

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Quantitative measurement of protein biomarkers is critical for biomarker validation and early disease detection. Current multiplex immunoassays are time consuming costly and can suffer from low accuracy. For example, multiplex ELISAs require multiple, tedious, washing and blocking steps. Moreover, they suffer from nonspecific antibody cross-reactions, leading to high background and falsepositive signals. Here, we show that co-localizing antibody-bead pairs in an aqueous two-phase system (ATPS) enables multiplexing of sensitive, no-wash, homogeneous assays, while preventing nonspecific antibody

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Raja, Selvaraj, and Vytla Ramachandra Murty. "Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology." Journal of Engineering 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/217483.

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Aqueous two-phase system (ATPS) composed of polyethylene glycol 6000 (PEG 6000) and sodium citrate (SC) has been proposed to recover the valuable soluble proteins from tannery wastewater. A sequential optimization strategy which included fractional factorial design (fFD) and central composite design (CCD) was employed to enhance the recovery. From this strategy, a second-order polynomial model was obtained for the protein recovery and it was validated. The optimum recovery was found as 93.46% when pH, NaCl concentration, and temperature were kept at 7.5, 0.1 M, and 33°C, respectively, for a ph

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Zhang, Zhi Feng, Rui Wang, Fei Ye, Haibin Wang, and Wenxia Zhao. "Extraction of glycyrrhizic acid by aqueous two-phase system formed by PEG and two environmentally friendly organic acid salts - sodium citrate and sodium tartrate." Green Processing and Synthesis 8, no. 1 (2019): 551–56. http://dx.doi.org/10.1515/gps-2019-0024.

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Abstract Two aqueous two-phase systems (ATPS’s) formed by using PEG and sodium citrate/sodium tartrate are applied and compared for extraction of glycyrrhizic acid (GA) from its stock solution. Their binodal curves and tie-lines are studied firstly. Influence of usage amount of the salt and the PEG on the partition coefficient and extraction efficiency is investigated. The highest extraction efficiency and partition coefficient achieved is 73% and 6.5, when the sodium citrate and PEG concentration is 15% and 20% respectively. It is also found that the ATPS based on sodium citrate is better tha

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