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Journal articles on the topic 'Aqueous two phase separation (ATPS)'

Author: Grafiati

Published: 9 September 2021

Last updated: 29 July 2025

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1

Ma, Chao, Xiao Dong Chen, Yin Qiu Kong, and Li Ming Che. "Phase Diagram of Aqueous Two-Phase System (ATPS) Composed of Polyethylene Glycol (PEG) and Gelatin." Advanced Materials Research 554-556 (July 2012): 286–94. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.286.

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Phase diagram of aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and gelatin is of paramount importance for the application of such system in microencapsulation of bioactive compounds. Phase separation of PEG/gelatin aqueous solution was investigated in the present work and the phase diagram of resultant ATPS was reported for the first time. The results show that phase separation will happen if the solid content of PEG/gelatin aqueous solution is higher than a critical value, resulting in an ATPS. The resultant ATPS consists of a low-density phase enriched in PEG and a de

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2

Atefi, Ehsan, Ramila Joshi, and Hossein Tavana. "Effect of Molecular Weight of Phase Polymers on Partition of Cells in Aqueous Two-Phase Systems." MRS Advances 2, no. 45 (2017): 2415–26. http://dx.doi.org/10.1557/adv.2017.425.

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ABSTRACTDue to their aqueous environment and biocompatible polymers, aqueous two-phase systems (ATPS) provide a mild environment for partition of cells. A comprehensive understanding of cell partition in ATPS will facilitate cell separation and fractionation in ATPS for downstream cell-based analytical applications and various cellular and molecular biology studies. We report the effect of molecular weight of phase polymers on partition of cells between two aqueous phases and their interface. We generate three different ATPS by dissolving polyethylene glycol and dextran of different molecular

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Rogers, Bradley A., Kelvin B. Rembert, Matthew F. Poyton, et al. "A stepwise mechanism for aqueous two-phase system formation in concentrated antibody solutions." Proceedings of the National Academy of Sciences 116, no. 32 (2019): 15784–91. http://dx.doi.org/10.1073/pnas.1900886116.

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Aqueous two-phase system (ATPS) formation is the macroscopic completion of liquid–liquid phase separation (LLPS), a process by which aqueous solutions demix into 2 distinct phases. We report the temperature-dependent kinetics of ATPS formation for solutions containing a monoclonal antibody and polyethylene glycol. Measurements are made by capturing dark-field images of protein-rich droplet suspensions as a function of time along a linear temperature gradient. The rate constants for ATPS formation fall into 3 kinetically distinct categories that are directly visualized along the temperature gra

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4

Titus, Amber R., Pedro P. Madeira, Vladimir N. Uversky, and Boris Y. Zaslavsky. "Anion Effect on Phase Separation of Polyethylene Glycol-8000–Sodium Salt Two-Phase Systems." Biophysica 4, no. 3 (2024): 357–68. http://dx.doi.org/10.3390/biophysica4030025.

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Aqueous two-phase systems (ATPSs) are formed when two nonionic polymers, or a single polymer and salt, are mixed in water above a specific concentration, resulting in the emergence of phase separation and the formation of two immiscible aqueous phases. The solvent properties of the aqueous media within the phases of ATPSs rely on the specific composition of the co-solutes and the arrangement of the hydrogen bond network within each phase. Here, we investigate the anion effect of various sodium salts on the enhancement or destabilization of polyethylene glycol (PEG)–salt ATPS formation. Relativ

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Kruse, Thomas, Axel Schmidt, Markus Kampmann, and Jochen Strube. "Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems." Antibodies 8, no. 3 (2019): 40. http://dx.doi.org/10.3390/antib8030040.

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Therapeutic monoclonal antibodies (mAb) are used for the treatment of numerous serious diseases, which have led to an increasing demand over the last decades. Increased cell density and mAb titer of the cultivation broth lead to great challenges for the subsequent clarification and capture operations in the downstream process. As an alternative approach to the conventional downstream process, a selective mAb extraction via an aqueous two-phase system (ATPS) directly from the cultivation broth of a mAb producing industrial relevant chinese hamster ovary (CHO) cell line was investigated. An effi

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Pereira, David Y., Chloe M. Wu, So Youn Lee, Eumene Lee, Benjamin M. Wu, and Daniel T. Kamei. "Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media." SLAS TECHNOLOGY: Translating Life Sciences Innovation 24, no. 5 (2019): 515–26. http://dx.doi.org/10.1177/2472630319861369.

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In previous work, our group discovered a phenomenon in which a mixed polymer–salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper. While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components. However, since the mechanism of phase separation in porous media is not com

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Souza, Ranyere L., Sónia P. M. Ventura, Cleide M. F. Soares, João A. P. Coutinho, and Álvaro S. Lima. "Lipase purification using ionic liquids as adjuvants in aqueous two-phase systems." Green Chemistry 17, no. 5 (2015): 3026–34. http://dx.doi.org/10.1039/c5gc00262a.

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8

Xu, Wenzhuo, Xinpei Gao, Liqiang Zheng, and Fei Lu. "Ionic-Liquid-Based Aqueous Two-Phase Systems Induced by Intra- and Intermolecular Hydrogen Bonds." Molecules 27, no. 16 (2022): 5307. http://dx.doi.org/10.3390/molecules27165307.

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In recent years, aqueous two-phase systems (ATPSs) have been widely used in different fields and have become an increasingly attractive subject due to their application in the separation and purification of biomolecules. In this work, the aqueous phase behavior of ionic liquids (ILs) was modulated by changing the cis-trans structure of the anion in ILs. With the same tetra-butyl-phosphine as the cation, the cis-anion exhibited upper critical solution temperature (UCST) phenomena. In contrast, the trans-anion exhibited lower critical solution temperature (LCST) phenomena. The proposed mechanism

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9

Dembczyński, Radosław, Wojciech Białas, and Tomasz Jankowski. "Determination of phase diagrams and thermoseparation behaviour of aqueous two-phase systems composed of ethylene oxide–propylene oxide random copolymer and potassium phosphate." Chemical and Process Engineering 33, no. 3 (2012): 411–29. http://dx.doi.org/10.2478/v10176-012-0036-8.

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Abstract The application of aqueous two-phase systems (ATPS) is a cost-effective and simple method of protein separation (including enzymes) from complex systems. The first stage of designing the protein purification process in an ATPS involves the identification of the conditions for the formation of a given extraction system. For this purpose, the conditions for the formation of ATPSs in a thermoseparating EO50PO50 polymer/potassium phosphates system have been studied. Factors determining the ATPS formation comprised: separation temperature (4ºC or 20ºC), phosphate solution pH (6, 7.5 or 9)

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10

Nie, Lirong, Ziwei Zheng, Mingxia Lu, Shun Yao, and Dong Guo. "Phase Behavior of Ionic Liquid-Based Aqueous Two-Phase Systems." International Journal of Molecular Sciences 23, no. 20 (2022): 12706. http://dx.doi.org/10.3390/ijms232012706.

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As an environmentally friendly separation medium, the ionic liquid (IL)-based aqueous two-phase system (ATPS) is attracting long-term attention from a growing number of scientists and engineers. Phase equilibrium data of IL-based ATPSs are an important basis for the design and optimization of chemical reactions and separation processes involving ILs. This article provides the recent significant progress that has been made in the field and highlights the possible directions of future developments. The effects of each component (such as salting-out agents and ILs) on the phase behavior of IL-bas

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11

Xie, Shaoqu, Zhenqi Sun, Jialin Tan, Wanli Zhang, Xiaolan Cai, and Yanxiong Fang. "Emerging Micromolecule-based Aqueous Two-phase Systems and Their Application in Biofuels." Innovation Discovery 1, no. 4 (2024): 30. http://dx.doi.org/10.53964/id.2024030.

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Aqueous two-phase system (ATPS) is a system composed of two incompatible solutions, which has excellent performance for extracting proteins, antibiotics, and other bioactive molecules, so it is a very simple, efficient, and green extraction and separation method. In this paper, we introduced three different emerging micromolecule-based ATPSs and introduced their characteristics, applications, advantages, and disadvantages respectively. This is of guiding significance for the synthesis of new ATPSs. One of the important prospects of this ATPS in industrial application is the separation and puri

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Fan, Linlin, Wenhui Li, Zilong Dai, Min Zhou, and Yunren Qiu. "Efficient Separation of Re (VII) and Mo (VI) by Extraction Using E-1006–Ammonium Sulfate Aqueous Two-Phase System." Separations 11, no. 5 (2024): 142. http://dx.doi.org/10.3390/separations11050142.

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Aqueous two-phase extraction (APTE) stands out as an environmentally friendly technique for the separation of metal ions. The separation of Re (VII) and Mo (VI) in an aqueous solution was investigated using a novel aqueous two-phase system (ATPS) consisting of isodecanol polyoxyethylene ether (E-1006), ammonium sulfate, and water. A phase diagram of this system was developed, and the effects of pH, temperature, extraction time, the concentrations of E-1006 and (NH4)2SO4, and metal ions on the separation of Re (VII) and Mo (VI) were examined. The results show that at pH 7.0, Mo (VI) had almost

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13

Gawade, Shubham, Sandeep P. Shewale, and Amravati Gode. "Aqueous Two-Phase Separation (ATPS) Methods for Oleic acid extraction from Neem leaves." IOP Conference Series: Materials Science and Engineering 1224, no. 1 (2022): 012016. http://dx.doi.org/10.1088/1757-899x/1224/1/012016.

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Abstract The aqueous two-phase separation system (ATPS) signifies an environmentally responsible approach for the extraction of bioactive compounds from a plants basis, as it is a liquid-liquid fractionation technique centred on the inconsistency of two aqueous solutions. In this investigation, various experimental parameters are optimized as the speed of agitation (200, 300 400 and 500 rpm) and solvent ratio (1:1, 2:3 and 3:2) with 20 % (w/w) of Ammonium Sulphate (AMS) salt composition and 30 % (w/w) of Polyethelyene Glycol (PEG). The obtained extract contains alkaloids, flavonoids, tannins,

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14

Seo, Hanjin, Changwoo Nam, Eunseo Kim, Juhyun Son, and Hyomin Lee. "Aqueous Two-Phase System (ATPS)-Based Polymersomes for Particle Isolation and Separation." ACS Applied Materials & Interfaces 12, no. 49 (2020): 55467–75. http://dx.doi.org/10.1021/acsami.0c16968.

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15

Wang, Wei Jie, Xiao Le Zhang, Chang Bo Xu, and Hong Yan Cheng. "Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System." Applied Mechanics and Materials 138-139 (November 2011): 995–1001. http://dx.doi.org/10.4028/www.scientific.net/amm.138-139.995.

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In this article, the partitioning behavior of C-phycocyanin (C-PC) in aqueous two-phase systems (ATPS) with different component was investigated to evaluate the applicability of ATPS for extracting high purity of C-PC without chromatography. Under the optimized conditions (pH = 6; PEG-2000, 16 %, w/w; potassium sodium tartrate 21 %, w/w), a maximum purity of 5.01 was achieved after the whole separation process and the yield of the C-PC was 76.94 %.

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Liu, Shao Peng, Zhi Min Zong, Shan Shan Zhang, et al. "Study of Aqueous Two Phase System on Water Soluble Bio Oil." Advanced Materials Research 236-238 (May 2011): 49–53. http://dx.doi.org/10.4028/www.scientific.net/amr.236-238.49.

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This paper was aimed to study the aqueous two phase extraction (ATPE) on water soluble bio oil(SBO), in which ethanol, K2HPO4, NaH2PO4and (NH4)2SO4were selected to form the water soluble bio oil(SBO) aqueous two phase system (ATPS). After phase separation, the extraction rates and the volume ratios of the upper to lower layer of the ATPSs were investigeted. Both the upper and lower layers of these SBO ATPSs were analyzed by elemental analyzer and Fourier transformation infrared spectrometer (FTIR). The different extraction rates indicates the influnces of different salt during the phase sapera

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Choi, Y. H., S. K. Mah, Y. S. Ng, and S. Y. Lee. "Fractionation of phycocyanin and carbohydrate from Spirulina platensis using ionic liquid-based aqueous two-phase system." IOP Conference Series: Materials Science and Engineering 1195, no. 1 (2021): 012039. http://dx.doi.org/10.1088/1757-899x/1195/1/012039.

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Abstract Microalgae have gained considerable attention due to their high-value biomolecules and potential applications in the pharmaceutical, food, medical and cosmeceutical field. However, the conventional biorefinery process of microalgae are costly, energy-intensive, and time-consuming. Aqueous two-phase system (ATPS) has emerged as a potential technique for the separation and fractionation of biomolecules in the biorefinery field. This study proposed the application of ionic liquid (IL) based ATPS for the fractionation of high-value phycocyanin and carbohydrates from a crude extract of Spi

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18

Wu, Jiang, Ying Kai Tong, and Li Tong Ban. "Separation of Casein Phosphopeptides (a Functional Food Material) by Aqueous Two-Phase Systems." Advanced Materials Research 554-556 (July 2012): 1511–14. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.1511.

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PEG 10000 / (NH4)2SO4 ATPS was applied to extract casein phosphopeptides. By studying on the partitioning behavior of CPPs in this system, it is concluded that the purification fold of CPPs could reach 7.09 when the system consisted of 20% PEG 10000 and 10% (NH4)2SO4.

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19

Jiang, Bin, Shaojing Zhong, Hongliang Yu, et al. "Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk." Separations 8, no. 11 (2021): 212. http://dx.doi.org/10.3390/separations8110212.

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Thiocyanate could effectively inhibit bacteria in milk and extend the shelf life of milk. However, excessive addition will lead to health risks. Therefore, the determination of thiocyanate in raw milk has received a lot of attention, but the determination could be interfered with by other components in raw milk and the pre-treatment of raw milk is complex. In this study, a new pretreatment method combined with ion chromatography (IC) for rapid and sensitive determination of thiocyanate is proposed. An acetonitrile/(NH4)2SO4 aqueous two-phase system (ATPS) was developed for the separation and e

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20

Wang, Yun, Juan Han, Xue-qiao Xie, and Chun-xiang Li. "Extraction of trace acetylspiramycin in real aqueous environments using aqueous two-phase system of ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate and phosphate." Open Chemistry 8, no. 6 (2010): 1185–91. http://dx.doi.org/10.2478/s11532-010-0080-5.

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AbstractThe partitioning of acetylspiramycin was carried out in an aqueous two-phase system (ATPS) formed by a hydrophilic ionic liquid (1-butyl-3-methylimidazolium tetrafluoraborate, [Bmim]BF4) and NaH2PO4. This ATPS is a simple, non-toxic and effective sample pretreatment technique, which was developed for the simultaneous separation, enrichment and rapid analysis of acetylspiramycin coupled with molecular fluorescence spectrophotometry. Analysis of the liquid-liquid equilibrium of [Bmim]BF4-salt ATPS demonstrated that the salting-out ability of different salts may be related to the Gibbs en

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21

Liu, Bangfu, and Zhijian Tan. "Separation and Purification of Astragalus membranaceus Polysaccharides by Deep Eutectic Solvents-Based Aqueous Two-Phase System." Molecules 27, no. 16 (2022): 5288. http://dx.doi.org/10.3390/molecules27165288.

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(1) Background: Aqueous two-phase systems (ATPSs) have been widely used in the separation and purification of bioactive substances in recent years. (2) Methods: In this study, deep eutectic solvents (DESs)-based ATPSs were employed for the extraction and separation of Astragalus membranaceus polysaccharides (AMP). The optimal DES (choline chloride:urea = 1:1) was first screened to extract AMP, and the effect of DES concentration, solid–liquid ratio, pH, extraction temperature, and extraction time on the extraction yield of AMP were investigated. (3) Results: The maximum extraction yield was 14

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Jugler, Collin, Jussi Joensuu, and Qiang Chen. "Hydrophobin-Protein A Fusion Protein Produced in Plants Efficiently Purified an Anti-West Nile Virus Monoclonal Antibody from Plant Extracts via Aqueous Two-Phase Separation." International Journal of Molecular Sciences 21, no. 6 (2020): 2140. http://dx.doi.org/10.3390/ijms21062140.

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The development of monoclonal antibodies (mAbs) has provided vast opportunities to treat a wide range of diseases from cancer to viral infections. While plant-based production of mAbs has effectively lowered the upstream cost of mAb production compared to mammalian cell cultures, further optimization of downstream processing, especially in extending the longevity of Protein A resin by an effective bulk separation step, will further reduce the overall prohibitive cost of mAb production. In this study, we explored the feasibility of using aqueous two-phase separation (ATPS) in capturing and sepa

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Li, Yuanyuan, Tongyu Li, Hongxu An, Xinyi Wang, Juan Han, and Yun Wang. "Extraction and separation of anthocyanins from Kushui rose by ethanol-(NH₄)₂SO₄ aqueous two-phase system." RSC Advances 14, no. 30 (2024): 21250–59. http://dx.doi.org/10.1039/d4ra03195d.

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Bekavac, Nikša, Maja Benković, Tamara Jurina, et al. "Advancements in Aqueous Two-Phase Systems for Enzyme Extraction, Purification, and Biotransformation." Molecules 29, no. 16 (2024): 3776. http://dx.doi.org/10.3390/molecules29163776.

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In recent years, the increasing need for energy conservation and environmental protection has driven industries to explore more efficient and sustainable processes. Liquid–liquid extraction (LLE) is a common method used in various sectors for separating components of liquid mixtures. However, the traditional use of toxic solvents poses significant health and environmental risks, prompting the shift toward green solvents. This review deals with the principles, applications, and advantages of aqueous two-phase systems (ATPS) as an alternative to conventional LLE. ATPS, which typically utilize wa

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Sun, Danyu, Runping Wang, Fenfang Li, Lianglei Liu, and Zhijian Tan. "Enantioselective Extraction of Phenylalanine Enantiomers Using Environmentally Friendly Aqueous Two-Phase Systems." Processes 6, no. 11 (2018): 212. http://dx.doi.org/10.3390/pr6110212.

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(1) Background: The environmentally friendly choline-amino acid ionic liquids (ChAAILs) and deep eutectic solvents (DESs) have been used as excellent alternatives to volatile organic compounds (VOCs) and ionic liquids (ILs) in recent years; (2) Methods: Thus, ChAAILs/salt and DESs/salt aqueous two-phase systems (ATPSs) were developed for the chiral extraction of phenylalanine enantiomers. The optimum ATPS of [Ch][L-Pro]/K3PO4 was chosen, and the influencing parameters were investigated, including ChAAILs concentration, salt concentration, chiral selector concentration, extraction temperature,

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Pericin, Draginja, Senka Madjarev-Popovic, and Zuzana Vastag. "Partitioning of acid protease from Penicillium roqueforti in aqueous two-phase system polyethylene glicol/phosphate." Acta Periodica Technologica, no. 39 (2008): 171–80. http://dx.doi.org/10.2298/apt0839171p.

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The partitioning of acid protease produced by submerged fermentation of Penicillium roqueforti in the aqueous two-phase system polyethylene glycol/sodium phosphate was studied. In the system with 15% polyethylene glycol 4000/25% NaH2PO4 favourable partition coefficient and the top phase yield were obtained, 2.651 and 56.51%, respectively. The purification of acid protease from crude enzyme was optimized by optimizing separation factor. Completely randomized factorial design was applied and the system 15.54% PEG 4000/20% NaH2PO4 appeared to be optimal. Statistically calculated separation factor

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Wu, Jiang, Li Tong Ban, and Yu Wang. "Separation of Curcumine (a Functional Food Colourant Material) from Rhizoma curcuma longae by Aqueous Two-Phase Systems." Applied Mechanics and Materials 217-219 (November 2012): 961–64. http://dx.doi.org/10.4028/www.scientific.net/amm.217-219.961.

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The objective of this paper was to separate curcumine from Rhizoma curcuma longae and aqueous two-phase extraction technology was employed to perform the study. Four types of PEG (400, 600, 2000 and 4000) and three types of salt ((NH4)2SO4, KH2PO4 and K2HPO4) were tested to form the aqueous two-phase systems. The results showed that the ATPS composed of PEG 400 and (NH4)2SO4 were the best. And the partition coefficient of curcumine could reach 21.79 when the concentration of PEG 400 and (NH4)2SO4 was 14.6% and 26% respectively.

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Kresheck, Gordon C., and Zheng Wang. "A new micellar aqueous two-phase partitioning system (ATPS) for the separation of proteins." Journal of Chromatography B 858, no. 1-2 (2007): 247–53. http://dx.doi.org/10.1016/j.jchromb.2007.08.037.

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Ahmed, Tasdiq, Cameron Yamanishi, Taisuke Kojima, and Shuichi Takayama. "Aqueous Two-Phase Systems and Microfluidics for Microscale Assays and Analytical Measurements." Annual Review of Analytical Chemistry 14, no. 1 (2021): 231–55. http://dx.doi.org/10.1146/annurev-anchem-091520-101759.

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Phase separation is a common occurrence in nature. Synthetic and natural polymers, salts, ionic liquids, surfactants, and biomacromolecules phase separate in water, resulting in an aqueous two-phase system (ATPS). This review discusses the properties, handling, and uses of ATPSs. These systems have been used for protein, nucleic acid, virus, and cell purification and have in recent years found new uses for small organics, polysaccharides, extracellular vesicles, and biopharmaceuticals. Analytical biochemistry applications such as quantifying protein–protein binding, probing for conformational

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Jiang, Bin, Jiaxin Na, Lele Wang, Dongmei Li, Chunhong Liu, and Zhibiao Feng. "Eco-Innovation in Reusing Food By-Products: Separation of Ovalbumin from Salted Egg White Using Aqueous Two-Phase System of PEG 1000/(NH4)2SO4." Polymers 11, no. 2 (2019): 238. http://dx.doi.org/10.3390/polym11020238.

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For the purpose of reducing pollution and the rational use of salted egg white, which is a byproduct of the manufacturing process of salted egg yolk, an aqueous two-phase system (ATPS) composed of polyethylene glycols (PEG 1000) and (NH4)2SO4 was investigated to selectively separate ovalbumin (OVA) from salted egg white. With the aim of optimizing the selective separation of OVA using ATPS, a response surface method (RSM) experiment was carried out on the basis of a single-factor experiment. The OVA was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE), reve

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Yang, Yu, Bilal Muhammad Khan, Xiping Zhang, Yongjie Zhao, Kit-Leong Cheong, and Yang Liu. "Advances in Separation and Purification of Bioactive Polysaccharides through High-speed Counter-Current Chromatography." Journal of Chromatographic Science 58, no. 10 (2020): 992–1000. http://dx.doi.org/10.1093/chromsci/bmaa063.

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Abstract Polysaccharides, with an extensive distribution in natural products, represent a group of natural bioactive substances having widespread applications in health-care food products and as biomaterials. Devising an efficient system for the separation and purification of polysaccharides from natural sources, hence, is of utmost importance in the widespread applicability and feasibility of research for the development of polysaccharide-based products. High-speed counter-current chromatography (HSCCC) is a continuous liquid–liquid partitioning chromatography with the ability to support a hi

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Zhao, Wen Yan, Jing Zhang, Xiang Ge, Yue Lu, and Rui Sheng Hu. "Extraction of Gatifloxacin in Water by Use of Ionic Liquid–Salt Aqueous Two-Phase System." Advanced Materials Research 356-360 (October 2011): 195–98. http://dx.doi.org/10.4028/www.scientific.net/amr.356-360.195.

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An ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate( [BMIM] BF4) / salt aqueous two-phase systems (ATPS) was presented as a simple, rapid and effective sample pretreatment technique coupled with double-beam UV-Vis spectrophotometer for analysis of gatifloxacin in water. For the purpose to choose optimal conditions, various factors were considered systematically, and the results indicated that the pH value, the salt-out ability of salt and the amount of [BMIM] BF4 had great inﬂuence on phase separation and extraction. Under the optimal condition, gatifloxacin in different concentrati

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Ni, Yunzhou, Zhixiang Qian, Yu Yin, Weien Yuan, Fei Wu, and Tuo Jin. "Polyvinyl Alcohol/Chitosan/Polyhexamethylene Biguanide Phase Separation System: A Potential Topical Antibacterial Formulation with Enhanced Antimicrobial Effect." Molecules 25, no. 6 (2020): 1334. http://dx.doi.org/10.3390/molecules25061334.

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An aqueous polyvinyl alcohol (PVA)/chitosan (CHT)/polyhexamethylene biguanide (PHMB) blends (PVA/CHT/PHMB blends) has been developed as a potential low dose topical antibacterial formulation with enhanced antimicrobial effect. The preparation of PVA/CHT/PHMB blends was quite facilely, with just dissolved PVA, CHT, PHMB in water in order. There was the aggregates with 100 nm size around induced by phase separation in the blends and an aqueous two-phase system (ATPS) formed, as non-ionic polymer PVA formed a continuous phase and cationic polymer CHT and PHMB formed dispersed phases. The minimum

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Duman, Yonca Avcı, Arda Acemi, Yonca Yuzugullu, and Fazıl Özen. "Separation of catalase from Amsonia orientalis with single step by aqueous two-phase partitioning system (ATPS)." Separation Science and Technology 52, no. 4 (2016): 691–99. http://dx.doi.org/10.1080/01496395.2016.1253588.

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Khan, Bilal Muhammad, Kit-Leong Cheong, and Yang Liu. "ATPS: “Aqueous two-phase system” as the “answer to protein separation” for protein-processing food industry." Critical Reviews in Food Science and Nutrition 59, no. 19 (2018): 3165–78. http://dx.doi.org/10.1080/10408398.2018.1486283.

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Maheshwari, Neha, Raj Kumar Arya, George D. Verros, Pradip B. Dhamole, and Ashwin Kannan. "Surfactant-Enhanced Extraction of Lutein from Marigold Petals using an Aqueous Two-Phase System." Separations 10, no. 2 (2023): 133. http://dx.doi.org/10.3390/separations10020133.

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The extraction of lutein from marigold petals using a surfactant-based aqueous two-phase system is reported. In this work, the effectiveness of the hydrophilic-lipophilic balance of surfactants on extraction performance for the extraction of lutein from marigold petal powder was demonstrated using aqueous solutions of a wide range of non-ionic surfactants. The response surface methodology was applied to obtain optimised conditions for maximum extraction of lutein. At the optimised conditions (Temperature = 37.5 °C, S/L = 0.00375, and surfactant amount = 1.5% (v/v)), 12.12 ± 0.16 mg/g of lutein

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Jakob, Lucas, Helene Katharina Baust, Lukas Griesinger, and Hermann Nirschl. "Optimized Apparatus Design for Continuous Aqueous Two-Phase Flotation (ATPF)." Separations 10, no. 9 (2023): 511. http://dx.doi.org/10.3390/separations10090511.

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Aqueous two-phase flotation (ATPF) is an integrative downstream method for separating and concentrating biomolecules from crude biosuspensions such as fermentation broth. Its continuous operation not only increases throughput, but also makes it recommended for continuous upstreams. In this work, an optimized apparatus design in the form of a horizontal flotation tank is presented. The geometry of the new apparatus for continuous ATPF is optimized using flow simulations so that the two aqueous phases can be pumped uniformly through the flotation tank in co-current or counter-current flow. The i

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Ali, Sajid, Sumera Afzal Khan, Muhammad Hamayun, and In-Jung Lee. "The Recent Advances in the Utility of Microbial Lipases: A Review." Microorganisms 11, no. 2 (2023): 510. http://dx.doi.org/10.3390/microorganisms11020510.

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Lipases are versatile biocatalysts and are used in different bioconversion reactions. Microbial lipases are currently attracting a great amount of attention due to the rapid advancement of enzyme technology and its practical application in a variety of industrial processes. The current review provides updated information on the different sources of microbial lipases, such as fungi, bacteria, and yeast, their classical and modern purification techniques, including precipitation and chromatographic separation, the immunopurification technique, the reversed micellar system, aqueous two-phase syst

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Mařík, Karel, Linda Tichá, Lucie Vobecká, and Michal Přibyl. "Theoretical study on enzyme synthesis of cephalexin in a parallel-flow microreactor combined with electrically driven ATPS microextraction." Reaction Chemistry & Engineering 5, no. 3 (2020): 570–83. http://dx.doi.org/10.1039/c9re00482c.

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A mathematical model of a microfluidic device with two aqueous phases for the simultaneous cephalexin production and its separation from a reaction mixture was developed. The model anticipates the continuous cephalexin synthesis and enzyme recyclation.

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Phyllis, N.Y. Otu, and Zhou Cunshan. "Deproteinization of Modelled Polysaccharides using Selected Alkyl Chain Length Ionic Liquid." Chemistry Research Journal 3, no. 6 (2018): 9–17. https://doi.org/10.5281/zenodo.13753868.

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Bovine serum albumen (BSA) at an isoelectric point is adsorbed on the surface of unmodified cellulose. This work sets out to research into the yield of modelled polysaccharides(cellulose) in a mimicked purification process (separation of modelled proteins, BSA, from modelled polysaccharides) using five different alkyl chain length of 1-alkyl-3-methylimidazolium chloride, [C<sub>n</sub>mim]Cl ionic liquids and inorganic salts, K<sub>2</sub>HPO<sub>4 </sub>/K<sub>2</sub>CO<sub>3 </sub>/ K<sub>3</sub>PO<sub>4</sub>in an aqueous two phase system. The solubility data was modelled using the Merchuck

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Valadares, Alberto, Clara França Valadares, Leandro Rodrigues de Lemos, Aparecida Barbosa Mageste, and Guilherme Dias Rodrigues. "Separation of cobalt and nickel in leach solutions of spent nickel-metal hydride batteries using aqueous two-phase systems (ATPS)." Hydrometallurgy 181 (November 2018): 180–88. http://dx.doi.org/10.1016/j.hydromet.2018.09.006.

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Dhadge, Vijaykumar L., Patricia I. Morgado, Filomena Freitas, et al. "An extracellular polymer at the interface of magnetic bioseparations." Journal of The Royal Society Interface 11, no. 100 (2014): 20140743. http://dx.doi.org/10.1098/rsif.2014.0743.

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FucoPol, a fucose-containing extracellular polysaccharide (EPS) produced by bacterium Enterobacter A47 using glycerol as the carbon source, was employed as a coating material for magnetic particles (MPs), which were subsequently functionalized with an artificial ligand for the capture of antibodies. The performance of the modified MPs (MP–EPS-22/8) for antibody purification was investigated using direct magnetic separation alone or combined with an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and dextran. In direct magnetic capturing, and using pure protein solutions o

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Ignatova, Tetyana, and Slava V. Rotkin. "(Invited) Demonstration of ATPS Phase Separation, B-Field Concentration and Manipulation of Sars-Cov-2 Viral Particles." ECS Meeting Abstracts MA2023-01, no. 9 (2023): 1157. http://dx.doi.org/10.1149/ma2023-0191157mtgabs.

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A technology, based on fluorescence sensing in a liquid sample and capable for an early detection of a pathogen at extremely low concentration, is very desirable though equally challenging to develop. In optical sensors, a typical fluorescent signal would be too weak to measure without special tools, unless the detected analyte, tagged with a fluorescent label, can be separated and concentrated. In this work a new combined technology[1] will be presented. An assembly of a monoclonal antibody (mAb) functionalized with a fluorescent tag with the S-proteins at the surface of inactivated coronavir

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Simanjuntak, Berlian, Helen Julian, and M. T. A. P. Kresnowati. "Downstream Process of Xylanase Production from Oil Palm Empty Fruit Bunches: A Review." IOP Conference Series: Earth and Environmental Science 1034, no. 1 (2022): 012046. http://dx.doi.org/10.1088/1755-1315/1034/1/012046.

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Abstract As an enzyme that can hydrolyze the glycosidic bond of hemicellulose, xylanase is widely used in pulp and paper, feed, food, and beverage industries. The vast availability of lignocellulosic biomass waste offers the potential of xylanase production by microorganisms via submerged and solid-state fermentation. However, the specific activity of the produced crude enzyme is still too low to be directly used in a commercial application. Downstream processing of the enzyme, that is purification and concentration, is necessary. Centrifugation or filtration can be used as a pre-treatment pro

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Ya Fedorov, A., A. V. Levina, and M. I. Fedorova. "Extraction of Eu(III), Gd(III), and Tb(III) in aqueous two-phase systems based on polyethylene glycol 1500-NaNO3-H2O with the addition of extractants (D2EHPA, TBP, TOMAN)." IOP Conference Series: Materials Science and Engineering 1212, no. 1 (2022): 012012. http://dx.doi.org/10.1088/1757-899x/1212/1/012012.

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Abstract Luminophores that used, for example, in fluorescent lamps, contain a large number of rare earth elements. Therefore, the processing of waste equipment containing luminophores is a rational approach to the obtaining of rare-earth metals, firstly, from the economics point of view, since they have a high cost, and secondly, from the ecological point of view, since environmental pollution will not occur. The cheapest way to extract rare earth elements from waste products is to dissolve them in strong acids and the following reprocessing by liquid extraction methods. In this case, neutral

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Zhang, Xunan, Mingxue Han, Shuang Han, and Wei Zong. "Aqueous two-phase system (ATPS): from basic science to applications." RSC Advances 15, no. 12 (2025): 9041–54. https://doi.org/10.1039/d4ra08232j.

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Moon, Byeong-Ui, Steven G. Jones, Dae Kun Hwang, and Scott S. H. Tsai. "Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures." Lab on a Chip 15, no. 11 (2015): 2437–44. http://dx.doi.org/10.1039/c5lc00217f.

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Choi, Daeho, Eunjeong Lee, Sung-Jin Kim, and Minsub Han. "Passive droplet generation in aqueous two-phase systems with a variable-width microchannel." Soft Matter 15, no. 23 (2019): 4647–55. http://dx.doi.org/10.1039/c9sm00469f.

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Vikram Gande, Vamsi, Hima Nandini K, Jagadeesh Korukonda, and S. Pushpavanam. "Hydrodynamics of aqueous two-phase systems (ATPS) in millichannels." Chemical Engineering Science 266 (February 2023): 118296. http://dx.doi.org/10.1016/j.ces.2022.118296.

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Martínez-Rodríguez, Diana Esmeralda, Edgar Daniel Martínez-Rodríguez, Liliana Hernández-Perales, María del Pilar González-Muñoz, and Mario Avila-Rodriguez. "Study of diclofenac distribution in aqueous two-phase PEG/salt/water systems." RSC Advances 15, no. 26 (2025): 21168–82. https://doi.org/10.1039/d5ra01070e.

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Diclofenac extraction was evaluated using Aqueous Two-Phase Systems (ATPS), achieving efficiencies exceeding 99.9% with PEG/citrate/water systems, highlighting their potential as a sustainable alternative for environmental remediation.

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